Journal articles: 'MSDI'

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Tavra, Marina, Vlado Cetl, and Tea Duplančić Leder. "A Framework for Evaluation of Marine Spatial Data Geoportals Using Case Studies." GeoScience Engineering 60, no. 4 (December 30, 2014): 9–18. http://dx.doi.org/10.1515/gse-2015-0002.

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Abstract Need for a Marine Spatial Data Infrastructure (MSDI) as a component of a National Spatial Data Infrastructure (NSDI) is widely recognized. An MSDI is relevant not only for hydrographers and government planners, but also for many other sectors which takes interest in marine spatial data, whether they are data users, data providers, or data managers [9]. An MSDI encompasses marine and coastal geographic and business information. For efficient use of Marine Spatial Data, it is necessary to ensure its valid and accessible distribution. A geoportal is a specialized web portal for sharing spatial information at different levels over the Internet. This paper re-examines the implementation of an MSDI and what it means for data custodians and end users. Several geoportals are reviewed (German and Australian) to determine their web services functionality, capabilities and the scope to which they support the sharing and reuse of Marine Spatial Data to assist the development of the Croatian MSDI Geoportal. This framework provides a context for better understanding the information bases on spatial data standards and a tool for evaluation of MSDI dissemination - Geoportal.

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Contarinis, Stilianos, Athanasios Pallikaris, and Byron Nakos. "The Value of Marine Spatial Open Data Infrastructures—Potentials of IHO S-100 Standard tο Become the Universal Marine Data Model." Journal of Marine Science and Engineering 8, no. 8 (July 27, 2020): 564. http://dx.doi.org/10.3390/jmse8080564.

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Marine spatial “open” data infrastructures (MSDI) have a significant economic and societal potential for coastal nations and their realization is driven by the evolution of the International Hydrographic Organization’s (IHO) S-100 data model for facilitating marine domain interoperability and the World Wide Web Consortium’s (W3C) best practices for spatial data publishing on the Web. The recent European directive on open data and the re-use of public sector information, known as the “Open Data Directive” is a key driver towards the establishment of “open” MSDIs among other spatial data infrastructures. The paper discusses possible data architectures for the MSDIs, examines the maturity of open data platforms that they could be built upon and compares the most prominent marine spatial data models for their applicability in relation to three marine information domains. MSDIs can facilitate the continuous data capturing of spatial-temporal physical phenomena and human activities at sea and coastal areas, the corresponding data analysis and the decision-making for achieving continual improvement in the marine planning and management processes. MSDIs could play a key role in digital government transformation (DGT) for effective data sharing and offering marine services across various stakeholders. The information provided through a MSDI can be used for safe and efficient operation of maritime traffic, exploration and exploitation of marine resources, marine spatial planning (MSP), integrated coastal zone management (ICZM), environmental protection, and naval and maritime security.

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Hao, Zengchao, and Amir AghaKouchak. "A Nonparametric Multivariate Multi-Index Drought Monitoring Framework." Journal of Hydrometeorology 15, no. 1 (February 1, 2014): 89–101. http://dx.doi.org/10.1175/jhm-d-12-0160.1.

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Abstract Accurate and reliable drought monitoring is essential to drought mitigation efforts and reduction of social vulnerability. A variety of indices, such as the standardized precipitation index (SPI), are used for drought monitoring based on different indicator variables. Because of the complexity of drought phenomena in their causation and impact, drought monitoring based on a single variable may be insufficient for detecting drought conditions in a prompt and reliable manner. This study outlines a multivariate, multi-index drought monitoring framework, namely, the multivariate standardized drought index (MSDI), for describing droughts based on the states of precipitation and soil moisture. In this study, the MSDI is evaluated against U.S. Drought Monitor (USDM) data as well as the commonly used standardized indices for drought monitoring, including detecting drought onset, persistence, and spatial extent across the continental United States. The results indicate that MSDI includes attractive properties, such as higher probability of drought detection, compared to individual precipitation and soil moisture–based drought indices. This study shows that the MSDI leads to drought information generally consistent with the USDM and provides additional information and insights into drought monitoring.

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Gavin, Daniel G. "Forest soil disturbance intervals inferred from soil charcoal radiocarbon dates." Canadian Journal of Forest Research 33, no. 12 (December 1, 2003): 2514–18. http://dx.doi.org/10.1139/x03-185.

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Forest soil disturbance intervals are usually too long to measure using plot-based studies, and thus they are poorly understood. The mean soil disturbance interval (MSDI) in an old-growth forest on the west coast of Vancouver Island was estimated from radiocarbon dates of charcoal from organic and mineral soil horizons. Two assumptions are required to estimate the MSDI: (1) charcoal from forest fires is deposited within the organic horizon and eventually mixed into deeper mineral horizons by soil disturbances, and (2) the probability of soil disturbance is spatially homogeneous and affected only by the time since the last fire or the last soil disturbance. The MSDI is then estimated by the rate at which the proportion of undisturbed sample sites (determined by the proportion of sites with charcoal from the most recent fire in the organic horizon) decreases with increasing time since the last fire. Soil charcoal evidence of time since fire was determined at 83 sites using 141 radiocarbon dates. The estimated MSDI was greater on slopes (ca. 2010 years) than on terraces (ca. 920 years). The long periods between soil disturbances, especially on slopes, are consistent with other evidence from the study area that suggests infrequent tree uprooting is the predominant mode of soil disturbance.

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Duvnjak, Ivan, Domagoj Damjanović, Marko Bartolac, and Ana Skender. "Mode Shape-Based Damage Detection Method (MSDI): Experimental Validation." Applied Sciences 11, no. 10 (May 18, 2021): 4589. http://dx.doi.org/10.3390/app11104589.

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The main principle of vibration-based damage detection in structures is to interpret the changes in dynamic properties of the structure as indicators of damage. In this study, the mode shape damage index (MSDI) method was used to identify discrete damages in plate-like structures. This damage index is based on the difference between modified modal displacements in the undamaged and damaged state of the structure. In order to assess the advantages and limitations of the proposed algorithm, we performed experimental modal analysis on a reinforced concrete (RC) plate under 10 different damage cases. The MSDI values were calculated through considering single and/or multiple damage locations, different levels of damage, and boundary conditions. The experimental results confirmed that the MSDI method can be used to detect the existence of damage, identify single and/or multiple damage locations, and estimate damage severity in the case of single discrete damage.

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Wang, Yunqian, Jing Yang, Yaning Chen, Zhicheng Su, Baofu Li, Hao Guo, and Philippe De Maeyer. "Monitoring and Predicting Drought Based on Multiple Indicators in an Arid Area, China." Remote Sensing 12, no. 14 (July 17, 2020): 2298. http://dx.doi.org/10.3390/rs12142298.

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Droughts are one of the costliest natural disasters. Reliable drought monitoring and prediction are valuable for drought relief management. This study monitors and predicts droughts in Xinjiang, an arid area in China, based on the three drought indicators, i.e., the Standardized Precipitation Index (SPI), the Standardized Soil Moisture Index (SSMI) and the Multivariate Standardized Drought Index (MSDI). Results indicate that although these three indicators could capture severe historical drought events in the study area, the spatial coverage, persistence and severity of the droughts would vary regarding different indicators. The MSDI could best describe the overall drought conditions by incorporating the characteristics of the SPI and SSMI. For the drought prediction, the predictive skill of all indicators gradually decayed with the increasing lead time. Specifically, the SPI only showed the predictive skill at a 1-month lead time, the MSDI performed best in capturing droughts at 1- to 2-month lead times and the SSMI was accurate up to a 3-month lead time owing to its high persistence. These findings might provide scientific support for the local drought management.

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Dlshad, Hawar Muhamad, Aziz Hama-Raheem Fatah, and Adil Mohammed Hussain. "The Elastic and Inelastic Electron-Nucleus Scattering Form Factors for Be9 Nucleus." UHD Journal of Science and Technology 4, no. 2 (August 17, 2020): 56. http://dx.doi.org/10.21928/uhdjst.v4n2y2020.pp56-62.

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The computations of the elastic and inelastic Coulomb form factors for the electron-nucleus scattering of Beryllium nucleus Be9 have performed with Core Polarization (CP) effects including the realistic Michigan sum of Three Range Yukawa (M3Y) Interaction, and the other residual interaction which is Modified Surface Delta Interaction (MSDI). In addition to mean square root charge density and charge radii for the ground state. The perturbation theory was adopted to compute the Core Polarization by using the Harmonic Oscillators (HO) potential to calculate single-particle radial wave functions. In the comparison between the theoretical calculations of Coulomb form factors by (MSDI) interaction, realistic (M3Y) interaction, and the experimental results that measured before, it noticed that the Coulomb form factors for the (M3Y) interaction gave a reasonable depiction of the measured data.

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Dampanaboina, Lavanya, Yinping Jiao, Junping Chen, Nicholas Gladman, Ratan Chopra, Gloria Burow, Chad Hayes, et al. "Sorghum MSD3 Encodes an ω-3 Fatty Acid Desaturase that Increases Grain Number by Reducing Jasmonic Acid Levels." International Journal of Molecular Sciences 20, no. 21 (October 28, 2019): 5359. http://dx.doi.org/10.3390/ijms20215359.

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Grain number per panicle is an important component of grain yield in sorghum (Sorghum bicolor (L.)) and other cereal crops. Previously, we reported that mutations in multi-seeded 1 (MSD1) and MSD2 genes result in a two-fold increase in grain number per panicle due to the restoration of the fertility of the pedicellate spikelets, which invariably abort in natural sorghum accessions. Here, we report the identification of another gene, MSD3, which is also involved in the regulation of grain numbers in sorghum. Four bulked F2 populations from crosses between BTx623 and each of the independent msd mutants p6, p14, p21, and p24 were sequenced to 20× coverage of the whole genome on a HiSeq 2000 system. Bioinformatic analyses of the sequence data showed that one gene, Sorbi_3001G407600, harbored homozygous mutations in all four populations. This gene encodes a plastidial ω-3 fatty acid desaturase that catalyzes the conversion of linoleic acid (18:2) to linolenic acid (18:3), a substrate for jasmonic acid (JA) biosynthesis. The msd3 mutants had reduced levels of linolenic acid in both leaves and developing panicles that in turn decreased the levels of JA. Furthermore, the msd3 panicle phenotype was reversed by treatment with methyl-JA (MeJA). Our characterization of MSD1, MSD2, and now MSD3 demonstrates that JA-regulated processes are critical to the msd phenotype. The identification of the MSD3 gene reveals a new target that could be manipulated to increase grain number per panicle in sorghum, and potentially other cereal crops, through the genomic editing of MSD3 functional orthologs.

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L. Hasyim, Sukarno. "MANAJEMEN SUMBER DAYA INSANI." PENDIDIKAN MULTIKULTURAL 4, no. 2 (August 13, 2020): 210. http://dx.doi.org/10.33474/multikultural.v4i2.7417.

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Pada dasarnya setiap organisasi tidak akan lepas dari keberadaan sumber daya insani yang dapat membantu melaksanakan serangkaizin aktivitas dalam pencapaian tujuan organisasi. Untuk itu diperlukan pula peran aktif manajer dalam memahami dan mengelola orang-orang yang ada dalam organisasi. Pengelolaan sumber daya insani harus dilakukan secara efektif dan efisien. Manajemen Sumber Daya Insani (MSDI) ini tidak saja mengandalkan pada fungsi manajemen seperti perencanaan, pengorganisasian, pengarahan dan pengendalian, namun pada implementasinya, mengandalkan pada fungsi operasional manajemen SDM seperti rekrutmen, seleksi, penilaian prestasi, pelatihan dan pengembangan, serta praktek pemberian kompensasi.Kata kunci: manajemen, fungsi, organisasi, keberadaan Basically, each organization will not be separated from the existence of human resources that can help carry out a series of activities in achieving organizational goals. This requires an active role of managers in understanding and managing people in the organization. Human resource management must be carried out effectively and efficiently. This Islamic Human Resource Management (MSDI) not only relies on management functions such as planning, organizing, directing and controlling, but in its implementation, relying on operational HR management functions such as recruitment, selection, performance appraisal, training and development, and the practice of compensation.Keywords: management, function, organization, existence

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Khan, Muhammad Imran, Xingye Zhu, Muhammad Arshad, Muhammad Zaman, Yasir Niaz, Ikram Ullah, Muhammad Naveed Anjum, and Muhammad Uzair. "Assessment of spatiotemporal characteristics of agro-meteorological drought events based on comparing Standardized Soil Moisture Index, Standardized Precipitation Index and Multivariate Standardized Drought Index." Journal of Water and Climate Change 11, S1 (October 15, 2020): 1–17. http://dx.doi.org/10.2166/wcc.2020.280.

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Abstract Drought indices that compute drought events by their statistical properties are essential stratagems for the estimation of the impact of drought events on a region. This research presents a quantitative investigation of drought events by analyzing drought characteristics, considering agro-meteorological aspects in the Heilongjiang Province of China during 1980 to 2015. To examine these aspects, the Standardized Soil Moisture Index (SSI), Standardized Precipitation Index (SPI), and Multivariate Standardized Drought Index (MSDI) were used to evaluate the drought characteristics. The results showed that almost half of the extreme and exceptional drought events occurred during 1990–92 and 2004–05. The spatiotemporal analysis of drought characteristics assisted in the estimation of the annual drought frequency (ADF, 1.20–2.70), long-term mean drought duration (MDD, 5–11 months), mean drought severity (MDS, −0.9 to −2.9), and mild conditions of mean drought intensity (MDI, −0.2 to −0.80) over the study area. The results obtained by MSDI reveal the drought onset and termination based on the combination of SPI and SSI, with onset being dominated by SPI and drought persistence being more similar to SSI behavior. The results of this study provide valuable information and can prove to be a reference framework to guide agricultural production in the region.

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Lou, Weimin, Pin Cao, Danhui Zhang, and Yongying Yang. "Optical Element Surface Defect Size Recognition Based on Decision Regression Tree." Applied Sciences 10, no. 18 (September 18, 2020): 6536. http://dx.doi.org/10.3390/app10186536.

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Defect size recognition is significant to the evaluation of optical element surface quality. Currently, it’s mainly achieved by the conventional image process, such as threshold segmentation. However, as the defect size gradually approaches the diffraction limit of the imaging system, the defect gray distribution changes from bimodal to unimodal, which makes it difficult to be accurately recognized. In this paper, an electromagnetic simulation model of the microscopic scattering dark-field imaging (MSDI) system is built based on the finite-difference time-domain (FDTD) method to research the defect imaging mechanism. The point spread function (PSF) of our MSDI system is measured to revise the far-field simulation light intensity distribution, and the mean value of the distance between three groups of feature points, whose intensity is 0.75, 0.5, and 0.25 of the light intensity distribution peak value, is taken as the feature parameter of the light intensity distribution. To obtain the defect size, the decision regression tree (DRT) is proposed to get the relationship between the feature parameter and the defect size. Besides, some scratches samples are made to verify the validity of the DRT. The results show the relative error of DRT is within 10%, which is better than the threshold segmentation.

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Heck, Thiago Gomes, Rafael Z. Frantz, Matias Nunes Frizzo, Carlos Henrique Ramires François, Mirna Stela Ludwig, Marilia Arndt Mesenburg, Giovano Pereira Buratti, Lígia Beatriz Bento Franz, and Evelise Moraes Berlezi. "Insufficient social distancing may contribute to COVID-19 outbreak: The case of Ijuí city in Brazil." PLOS ONE 16, no. 2 (February 17, 2021): e0246520. http://dx.doi.org/10.1371/journal.pone.0246520.

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The coronavirus disease that emerged in 2019 (COVID-19) is highly contagious and has given way to a global pandemic. A present COVID-19 has high transmission rates worldwide, including in small Brazilian cities such as Ijuí. Located in the northwest part of the state of Rio Grande do Sul (RS) and with a population of 83,475, Ijuí was selected as the site of a population-based survey involving 2,222 subjects, from April to June 2020. Subjects were tested for the presence of antibodies against coronavirus (SARS-CoV-2) and answered questions regarding social distance adherence (SDA), daily preventive routines (DPR), comorbidities, and sociodemographic characteristics. In parallel, the local government registered the official COVID-19 cases in Ijuí, as well as the mobile social distancing index (MSDI). In this study, we demonstrate that there was a decrease in the levels of SDA, DPR and MSDI before the beginning of COVID-19 community transmission in Ijuí. Furthermore, we provide predictions for the number of COVID-19 cases, hospitalizations, and deaths in the city. We conclude that insufficient social distancing, as evidenced by different methods, may be related to the rapid increase of COVID-19 cases in Ijuí. Our study predicts an approaching outbreak of COVID-19 in Ijuí through community spread, which could be avoided or attenuated with increased levels of social distancing among the population.

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Ren, Hong Yu, Diane E. Grove, Oxana De La Rosa, Scott A. Houck, Pattarawut Sopha, Fredrick Van Goor, Beth J. Hoffman, and Douglas M. Cyr. "VX-809 corrects folding defects in cystic fibrosis transmembrane conductance regulator protein through action on membrane-spanning domain 1." Molecular Biology of the Cell 24, no. 19 (October 2013): 3016–24. http://dx.doi.org/10.1091/mbc.e13-05-0240.

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Cystic fibrosis (CF) is a fatal genetic disorder associated with defective hydration of lung airways due to the loss of chloride transport through the CF transmembrane conductance regulator protein (CFTR). CFTR contains two membrane-spanning domains (MSDs), two nucleotide-binding domains (NBDs), and a regulatory domain, and its channel assembly requires multiple interdomain contacts. The most common CF-causing mutation, F508del, occurs in NBD1 and results in misfolding and premature degradation of F508del-CFTR. VX-809 is an investigational CFTR corrector that partially restores CFTR function in people who are homozygous for F508del-CFTR. To identify the folding defect(s) in F508del-CFTR that must be repaired to treat CF, we explored the mechanism of VX-809 action. VX-809 stabilized an N-terminal domain in CFTR that contains only MSD1 and efficaciously restored function to CFTR forms that have missense mutations in MSD1. The action of VX-809 on MSD1 appears to suppress folding defects in F508del-CFTR by enhancing interactions among the NBD1, MSD1, and MSD2 domains. The ability of VX-809 to correct F508del-CFTR is enhanced when combined with mutations that improve F508del-NBD1 interaction with MSD2. These data suggest that the use of VX-809 in combination with an additional CFTR corrector that suppresses folding defects downstream of MSD1 may further enhance CFTR function in people with F508del-CFTR.

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Xu, Yongfeng, and Weidong Zhu. "Non-model-based damage identification of plates using measured mode shapes." Structural Health Monitoring 16, no. 1 (December 15, 2016): 3–23. http://dx.doi.org/10.1177/1475921716655974.

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Mode shapes (MSs) have been extensively used to detect structural damage. This paper presents a new non-model-based damage identification method that uses measured MSs to identify damage in plates. A MS damage index (MSDI) is proposed to identify damage near regions with consistently high values of MSDIs associated with MSs of different modes. A MS of a pseudo-undamaged plate can be constructed for damage identification using a polynomial of a properly determined order that fits the corresponding MS of a damaged plate, if the associated undamaged plate is geometrically smooth and made of materials that have no stiffness and mass discontinuities. It is shown that comparing a MS of a damaged plate with that of a pseudo-undamaged plate is better for damage identification than with that of an undamaged plate. Effectiveness and robustness of the proposed method for identifying damage of different positions and areas are numerically investigated using different MSs; effects of crucial factors that determine effectiveness of the proposed method are also numerically investigated. Damage in the form of a machined thickness reduction area was introduced to an aluminum plate; it was successfully identified by the proposed method using measured MSs of the damaged plate.

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Bouvet, M., V. Parra, and J. M. Suisse. "Molecular semiconductor-doped insulator (MSDI) heterojunctions as new transducers for chemical sensors." European Physical Journal Applied Physics 56, no. 3 (November 23, 2011): 34103. http://dx.doi.org/10.1051/epjap/2011110220.

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Parra, Vicente, Jérôme Brunet, Alain Pauly, and Marcel Bouvet. "Molecular semiconductor-doped insulator (MSDI) heterojunctions: an alternative transducer for gas chemosensing." Analyst 134, no. 9 (2009): 1776. http://dx.doi.org/10.1039/b906786h.

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Rúa-Figueroa, I., D. Rua-Figueroa, A. M. Anzola Alfaro, N. Pérez-Veiga, M. Galindo-Izquierdo, J. Calvo-Alén, A. Fernandez-Nebro, et al. "POS0721 ARE ANTIMALARIALS SAFE FOR THE HEART OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS? ANALYSIS OF FACTORS ASSOCIATED WITH THE DEVELOPMENT OF HEART FAILURE IN PATIENTS IN THE SPANISH SOCIETY OF RHEUMATOLOGY LUPUS REGISTRY (RELESSER)." Annals of the Rheumatic Diseases 80, Suppl 1 (May 19, 2021): 610–11. http://dx.doi.org/10.1136/annrheumdis-2021-eular.1208.

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Background:Factors associated with the development of chronic heart failure (CHF) in systemic lupus erythematosus (SLE) have received little attention. On the other hand, recent data from the use of hydroxychloroquine in the treatment of SARS-CoV-2 infection during the COVID19 pandemic have cast some doubts on its cardiological safety.Objectives:To identify factors associated to CHF in SLE.Methods:Retrospective cross-sectional study, including all patients with SLE (≥4 ACR-1997 criteria) recruited in RELESSER registry. The objectives and methodology of the registry have been described previously (1). CHF was defined according to the Charlson index item. Patients with CHF before diagnosis of SLE were excluded. Cumulative damage was measured with the SLICC/ACR index, excluding cardiovascular (CV) items (mSDI). Multivariate analysis exploring factors associated with CHF was carried out.Results:117 patients (3% of the entire cohort) with SLE and CHF and 3,506 controls with SLE without CHF were included. 90% were women. Disease duration: mean (SD), 120.2 (87.7) months. CHF appeared after a median (P25-P75) of 9.40 (4.2-18.3) years from SLE diagnosis. Patients with CHF were older (59.8 ± 18.2 vs. 46.2 ± 4.3). In the bivariate analysis, the association of CHF with greater severity [Katz severity index: median (IQR): 4 (3-5) vs. 2 (1-3)], damage [mSDI: 3 (2-4) vs 0 (0-1)], comorbidity [modified Charlson- excluding CV items: 4 (3-6) vs 1(1-3)] and both CV (37.5% vs 6.7%) and overall mortality (43.2% vs 4.7%) (p<0.0001 for all comparisons). Also, CHF patients were more refractory to SLE treatments (33.3% vs 24%, p=0.0377) and were more frequently hospitalised due SLE [median 3 (1-5) vs 1(0-2), p<0.0001]. The results of the multivariable model are depicted in table 1.Table 1.Congestive heart failure associated factors (multivariable analysis)Odds Ratio95% CIP-valueSex (female)0.460.25 - 0.880.0147Ischaemic cardiopathy7.964.01 - 15.48<0.0001Cardiac arrhythmia7.384.00 - 13.42<0.0001Pulmonary hypertension3.711.84 - 7.250.0002Cardiac valvulopathy6.333.41 - 11.62<0.0001Hospitalization (due to SLE)3.741.81 - 8.650.0008Calcium or vitamin D5.292.07 - 16.860.0015Antimalarials0.280.17 - 0.45<0.0001mSDI *1.291.16 - 1.44<0.0001*mSDI = modified SLICC/ACR damage index (without cardiovascular items)Conclusion:- CHF is a rather late complication of SLE.- Patients with SLE and CHF have more severe SLE, with greater refractoriness to SLE treatments and higher overall mortality.- Treatment with antimalarials, as routinely used in SLE patients, is not only safe to heart, but even appears to have a cardioprotective effect.References:[1]Rúa-Figueroa I, López-Longo FJ, Calvo-Alén J, et al. National registry of patients with systemic lupus erythematosus of the Spanish Society of Rheumatology: objectives and methodology. Reumatol Clin. 2014;10(1):17-24.Acknowledgements:Research Unit of Spanish Society of RheumatologyDisclosure of Interests:None declared

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Gaudillat, Pierre, Jean Moïse Suisse, and Marcel Bouvet. "Humidity Insensitive Conductometric Sensors for Ammonia Sensing." Key Engineering Materials 605 (April 2014): 181–84. http://dx.doi.org/10.4028/www.scientific.net/kem.605.181.

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Interest in molecular materials has been driven in large part by their various and prosperous applications, especially in the domain of organic electronics, where they offer many advantages as well as alternative approaches compared to their inorganic counterparts. Most of conductometric transducers are resistors[[ and transistors[[[, but rarely diodes[6]. In our laboratory, we designed and characterized new molecular material based devices. Molecular Semiconductor Doped Insulator (MSDI) heterojunctions were built around a heterojunction between a Molecular Semiconductor (MS) and a Doped Insulator (DI)[7][8]. This new device exhibits interesting electronic properties that allow ammonia sensing in a large humidity range at room temperature.

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Dlshad, Hawar Muhamad, and Aziz Hama Raheem Fatah. "Using MSDI and M3Y Core Polarization for the Coulomb Electron Scattering for some Ground state nuclei." Journal of Zankoy Sulaimani - Part A 21, no. 2 (September 4, 2019): 11–20. http://dx.doi.org/10.17656/jzs.10753.

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Koike, Koji, Curtis J. Oleschuk, Anass Haimeur, Sharon L. Olsen, Roger G. Deeley, and Susan P. C. Cole. "Multiple Membrane-associated Tryptophan Residues Contribute to the Transport Activity and Substrate Specificity of the Human Multidrug Resistance Protein, MRP1." Journal of Biological Chemistry 277, no. 51 (October 17, 2002): 49495–503. http://dx.doi.org/10.1074/jbc.m206896200.

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The multidrug resistance protein, MRP1, is a clinically important ATP-binding cassette transporter in which the three membrane-spanning domains (MSDs), which contain up to 17 transmembrane (TM) helices, and two nucleotide binding domains (NBDs) are configured MSD1-MSD2-NBD1-MSD3-NBD2. In tumor cells, MRP1 confers resistance to a broad spectrum of drugs, but in normal cells, it functions as a primary active transporter of organic anions such as leukotriene C4and 17β-estradiol 17β-(d-glucuronide). We have previously shown that mutation of TM17-Trp1246eliminates 17β-estradiol 17β-(d-glucuronide) transport and drug resistance conferred by MRP1 while leaving leukotriene C4transport intact. By mutating the 11 remaining Trp residues that are in predicted TM segments of MRP1, we have now determined that five of them are also major determinants of MRP1 function. Ala substitution of three of these residues, Trp445(TM8), Trp553(TM10), and Trp1198(TM16), eliminated or substantially reduced transport levels of five organic anion substrates of MRP1. In contrast, Ala substitutions of Trp361(TM7) and Trp459(TM9) caused a more moderate and substrate-selective reduction in MRP1 function. More conservative substitutions (Tyr and Phe) of the Trp445, Trp553, and Trp1198mutants resulted in substrate selective retention of transport in some cases (Trp445and Trp1198) but not others (Trp553). Our findings suggest that the bulky polar aromatic indole side chain of each of these five Trp residues contributes significantly to the transport activity and substrate specificity of MRP1.

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Chen, Yanli, Marcel Bouvet, Thibaut Sizun, Guillaume Barochi, Jérôme Rossignol, and Eric Lesniewska. "Enhanced chemosensing of ammonia based on the novel molecular semiconductor-doped insulator (MSDI) heterojunctions." Sensors and Actuators B: Chemical 155, no. 1 (July 2011): 165–73. http://dx.doi.org/10.1016/j.snb.2010.11.042.

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Majeed1, Firas Zuhair, ,. Ammar A. Battawy2, and Muhanned A. Ahmed2. "Nuclear energy levels in 58Ni." Tikrit Journal of Pure Science 24, no. 3 (May 9, 2019): 100. http://dx.doi.org/10.25130/j.v24i3.824.

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Energy levels, total angular momenta and parity for two nucleons that present outside closed core 56Ni (Nickel) which occupied FP nuclear shell have been calculated using nuclear shell model application. We have used FP M3YE interaction to calculate energy levels of 58Ni, in addition, the results are compared with experimental data, modified surface delta interaction (MSDI), Reid's potential (RP) and non-zero pairing shell model (NZPSM). Harmonic Oscillator potential is used to generate single particle wave functions in FP shell and considering as an inert core. Oxford Buenos Aires Shell Model (OXBASH) code for nuclear shell model calculation has been utilized to carry out the calculations and comparison with experimental data had been done. http://dx.doi.org/10.25130/tjps.24.2019.054

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Hasan, Ali Khalaf, and Dalal Naji Hameed. "Energy Levels of 50Ca Nucleus as a Function of the Classical Coupling Angle within MSDI." NeuroQuantology 19, no. 5 (June 10, 2021): 61–67. http://dx.doi.org/10.14704/nq.2021.19.5.nq21049.

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In the construction of this kind of shell model, we take the residual interaction to be modified surface delta interaction MSDI. We have studied the excitation energies of the 50Ca a nucleus, which contain two neutrons outside closed shell of the 48Ca. Neutrons are in the model space pfpg. The energy levels and angular momentum of all possible cases were investigated. Thereby, we have effectively utilized a theoretical process to find link among the traditional coupling angle and energy levels at different orbital within neutron - neutron interaction. We observe the energy stages appear to follow two overall functions which depend on the classical coupling angles but are unconstrained of angular momentum I. We find out that our results agree with the experimental data.

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Gaudillat, Pierre, Amélie Wannebroucq, Jean-Moïse Suisse, and Marcel Bouvet. "Bias and humidity effects on the ammonia sensing of perylene derivative/lutetium bisphthalocyanine MSDI heterojunctions." Sensors and Actuators B: Chemical 222 (January 2016): 910–17. http://dx.doi.org/10.1016/j.snb.2015.09.015.

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LEE, MING-YIH, CHIH-FENG LIN, and KOK-SOON SOON. "DESIGN OF A NEW BIOFEEDBACK PROPRIOCEPTIVE NEUROMUSCULAR FACILITATION SYSTEM FOR BELOW-KNEE AMPUTEES." Biomedical Engineering: Applications, Basis and Communications 18, no. 04 (August 25, 2006): 190–97. http://dx.doi.org/10.4015/s1016237206000300.

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Proprioceptive neuromuscular facilitation and foot sensory compensation are critical to balance control and ambulatory performance in below-knee amputees. Sub-sensory stimulation has been shown to be effective in enhancing the sensitivity of the human somatosensory system. In addition, visual-auditory biofeedback to improve foot sensory compensation for amputees was suggested in recent articles. The purpose of this study is to develop a new biofeedback proprioceptive neuromuscular facilitation system for improving balance control and foot sensory compensation in below-knee amputees. The proposed system functioned with sub-threshold electrical stimulation and visual-auditory biofeedback was developed for clinical study. Two unilateral trans-tibial amputees who consecutively wore prosthetics over 10 years were participated in this study. Subjects performed multiple single leg quite standing trails with sub-sensory electrical stimulation applied at the quadriceps muscle during half of the trails. Four static balance performance indices (i.e. Holding Time Index, HTI; Sway Length Index, SLI; Max Sway Distance Index, MSDI; Average Sway Distance Index, ASDI) were characterized using Zebris motion analysis system. The improvement ratio of these static balance performance indices across subjects for single leg quiet standing tests were resulted in a 209.7% in HTI, 39.1% in SLI, 24.3% in MSDI, and 65.4% in ASDI respectively. In addition, multiple treadmill ambulatory trails with or without visual-auditory biofeedback were evaluated. Four dynamic gait performance indices (i.e. Double Support Time Index, DSTI; Constant Time Cadence Index, CTCI; Single Support Time Index, SSTI; Stance/Swing Phase Index, SSPI) were characterized with Zebris instrumented insole and associated FMS analysis software. With visual-auditory biofeedback, the improvement of all four dynamic gait performance indices in below-knee amputees was verified. The improvement ratio of four gait performance indices across subjects resulted in a 14.81% in DSTI (sound side), 14.29% in DSTI (affected side), 14% in CTCI, 13.00% in SSTI (sound side), 6.02% in SSTI (affected side), 45.17% in SSPI (sound side), and 27.49% in SSPI (affected side) respectively. These findings suggest that sub-threshold electrical stimulation and visual-auditory biofeedback proprioceptive neuromuscular facilitation strategies may be effective in compensating foot sensory loss and improving balance control for below-knee amputees.

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Lee, Ming-Yih, Chih-Feng Lin, and Kok-Soon Soon. "Balance control enhancement using sub-sensory stimulation and visual-auditory biofeedback strategies for amputee subjects." Prosthetics and Orthotics International 31, no. 4 (December 2007): 342–52. http://dx.doi.org/10.1080/03093640601058162.

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Sub-sensory electrical or mechanical stimulation can enhance the sensitivity of the human somatosensory system to improve the balance control capabilities of elderly. In addition, clinical studies suggest that visual-auditory biofeedback can improve sensory compensation for the elderly. This study hypothesizes that the static balance and gait performance of single leg quiet standing and treadmill walking could be improved for providing proprioceptive neuromuscular facilitation using sub-sensory stimulation and visual-auditory biofeedback in amputee subjects. To test this, a computerized foot pressure biofeedback sensory compensation system using sub-threshold low-level electrical stimulation combined with visual-auditory biofeedback was developed. Seven unilateral trans-tibial amputees who wore prostheses over 2 years were recruited. The subjects performed multiple single leg quiet standing trials with sub-sensory electrical stimulation applied at the quadriceps muscle during half of the trials. Static balance performance was characterized by using a Zebris motion analysis system to measure the sway distance and duration of the centre of mass on the second sacral (S2) of the subjects. In addition, multiple treadmill ambulatory trials with or without visual-auditory biofeedback was performed. Dynamic gait performance was characterized with a Zebris instrumented insole to measure the temporal responses of foot pressure sensors. Experimental results showed an improvement in three balance performance indices (Holding Time Index, HTI, Maximum Sway Distance Index, MSDI, and Average Sway Distance Index, ASDI) during single leg quiet standing by applying sub-sensory stimulation. The improvement ratio of these balance performance indices across subjects for single leg quiet standing tests resulted in 132.34% in HTI, 44.61% in MSDI, and 61.45% in ASDI. With visual-auditory biofeedback as a cue for heel contact and toe push-off condition during treadmill ambulation, the improvement of four dynamic gait performance measures (Double Support Period, DSP, Constant Time Cadence, CTC, Single Support Period, SSP, and Stance/Swing Ratio, SSR) in amputees was verified. This resulted in 7.89% in DSP (affected side), 5.09% in CTC, 16.67% in SSP (sound side), 45.30% in SSR (sound side), and 40.30% in SSR (affected side) respectively. These findings suggest that sub-threshold electrical stimulation and visual-auditory biofeedback rehabilitation strategies may be effective in compensating sensory loss and improving static balance and dynamic ambulation performance for amputees.

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Bouvet, Marcel, Pierre Gaudillat, Abhishek Kumar, Tilman Sauerwald, Marco Schüler, Andreas Schütze, and Jean-Moïse Suisse. "Revisiting the electronic properties of Molecular Semiconductor – Doped Insulator (MSDI) heterojunctions through impedance and chemosensing studies." Organic Electronics 26 (November 2015): 345–54. http://dx.doi.org/10.1016/j.orgel.2015.07.052.

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Seip, Christian, and Ralf Bill. "A Framework for the Evaluation of Marine Spatial Data Infrastructures – Accompanied by International Case-Studies." GeoScience Engineering 62, no. 2 (June 1, 2016): 27–43. http://dx.doi.org/10.1515/gse-2016-0015.

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Abstract Germany is currently developing a marine data infrastructure addressing different topics such as coastal engineering, hydrography and surveying, protection of the marine environment, maritime conservation, regional planning, and coastal research. This undertaking is embedded in a series of regulations and developments at many administrative levels, from which specifications and courses of action are derived. To set up a conceptual framework for the marine data infrastructure of Germany (MDI-DE), scientists at the Chair for Geodesy and Geoinformatics at the Rostock University are building a reference model, evaluating meta-information systems and developing models to support common workflows in marine applications. Evaluating how other countries built their marine spatial infrastructures is important to learn where obstacles and errors are likely to occur. To be able to look at other initiatives from a neutral point of view, it is necessary to construct a framework for evaluating marine spatial data infrastructures (MSDI). This framework is then used to analyse and evaluate the efforts of Canada, Australia, and Ireland with respect to marine data infrastructures.

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Majeed, Fouad A., and And Sarah M. Obaid. "Nuclear structure study of [22,24]Ne and [24]Mg nuclei." Revista Mexicana de Física 65, no. 2 (March 26, 2019): 159. http://dx.doi.org/10.31349/revmexfis.65.159.

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Shell model calculations based on large basis has been conducted to study the nuclear structure of $^{20}Ne$, $^{22}Ne$ and $^{24}Mg$ nuclei. The energy levels, inelastic electron scattering form factors and transition probabilities are discussed by considering the contribution of configurations with high-energy beyond the model space of sd-shell model space which is denoted as the core polarization (CP) effects.~The Core polarization is considered by taking the excitations of nucleus from the $1s$ and $1p$ core orbits and also from the valence $2s$ $1d$ shell orbit in to higher shells with $4\hbar\omega$. The effective interactions $USDA$ and $USDB$ are employed with $sd$ shell model space to perform the calculation and the core polarization are calculated with $MSDI$ as residual interaction.~The calculated energy level schemes, form factors and transition probabilities were compared with the corresponding experimental data. The effect of core polarization is found very important for the calculation of $B(C2)$, $B(C4)$ and form factors, and gives excellent results in comparison with the experimental data without including any adjustable parameters.

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Bouvet, Marcel, Hui Xiong, and Vicente Parra. "Molecular semiconductor-doped insulator (MSDI) heterojunctions: Oligothiophene/bisphtalocyanine (LuPc2) and perylene/bisphthalocyanine as new structures for gas sensing." Sensors and Actuators B: Chemical 145, no. 1 (March 4, 2010): 501–6. http://dx.doi.org/10.1016/j.snb.2009.12.064.

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Rosser, Meredith F. N., Diane E. Grove, Liling Chen, and Douglas M. Cyr. "Assembly and Misassembly of Cystic Fibrosis Transmembrane Conductance Regulator: Folding Defects Caused by Deletion of F508 Occur Before and After the Calnexin-dependent Association of Membrane Spanning Domain (MSD) 1 and MSD2." Molecular Biology of the Cell 19, no. 11 (November 2008): 4570–79. http://dx.doi.org/10.1091/mbc.e08-04-0357.

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Cystic fibrosis transmembrane conductance regulator (CFTR) is a polytopic membrane protein that functions as a Cl− channel and consists of two membrane spanning domains (MSDs), two cytosolic nucleotide binding domains (NBDs), and a cytosolic regulatory domain. Cytosolic 70-kDa heat shock protein (Hsp70), and endoplasmic reticulum-localized calnexin are chaperones that facilitate CFTR biogenesis. Hsp70 functions in both the cotranslational folding and posttranslational degradation of CFTR. Yet, the mechanism for calnexin action in folding and quality control of CFTR is not clear. Investigation of this question revealed that calnexin is not essential for CFTR or CFTRΔF508 degradation. We identified a dependence on calnexin for proper assembly of CFTR's membrane spanning domains. Interestingly, efficient folding of NBD2 was also found to be dependent upon calnexin binding to CFTR. Furthermore, we identified folding defects caused by deletion of F508 that occurred before and after the calnexin-dependent association of MSD1 and MSD2. Early folding defects are evident upon translation of the NBD1 and R-domain and are sensed by the RMA-1 ubiquitin ligase complex.

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Young, Karen W. H., Barbara Danielewska-Nikiel, and Ian C. Munro. "An evaluation of the maximized survey-derived daily intake (MSDI) as a practical method to estimate intake of flavouring substances." Food and Chemical Toxicology 44, no. 11 (November 2006): 1849–67. http://dx.doi.org/10.1016/j.fct.2006.06.004.

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Nelson, Flavia, Mohammad A. Akhtar, Edward Zúñiga, Carlos A. Perez, Khader M. Hasan, Jeffrey Wilken, Jerry S. Wolinsky, Ponnada A. Narayana, and Joel L. Steinberg. "Novel fMRI working memory paradigm accurately detects cognitive impairment in multiple sclerosis." Multiple Sclerosis Journal 23, no. 6 (September 9, 2016): 836–47. http://dx.doi.org/10.1177/1352458516666186.

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Background: Cognitive impairment (CI) cannot be diagnosed by magnetic resonance imaging (MRI). Functional magnetic resonance imaging (fMRI) paradigms, such as the immediate/delayed memory task (I/DMT), detect varying degrees of working memory (WM). Preliminary findings using I/DMT showed differences in blood oxygenation level dependent (BOLD) activation between impaired (MSCI, n = 12) and non-impaired (MSNI, n = 9) multiple sclerosis (MS) patients. Objectives: The aim of the study was to confirm CI detection based on I/DMT BOLD activation in a larger cohort of MS patients. The role of T2 lesion volume (LV) and Expanded Disability Status Scale (EDSS) in magnitude of BOLD signal was also sought. Methods: A total of 50 patients (EDSS mean ( m) = 3.2, disease duration (DD) m = 12 years, and age m = 40 years) underwent the Minimal Assessment of Cognitive Function in Multiple Sclerosis (MACFIMS) and I/DMT. Working memory activation (WMa) represents BOLD signal during DMT minus signal during IMT. CI was based on MACFIMS. Results: A total of 10 MSNI, 30 MSCI, and 4 borderline patients were included in the analyses. Analysis of variance (ANOVA) showed MSNI had significantly greater WMa than MSCI, in the left prefrontal cortex and left supplementary motor area ( p = 0.032). Regression analysis showed significant inverse correlations between WMa and T2 LV/EDSS in similar areas ( p = 0.005, 0.004, respectively). Conclusion: I/DMT-based BOLD activation detects CI in MS. Larger studies are needed to confirm these findings.

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Du, Kai, and Gergely L. Lukacs. "Cooperative Assembly and Misfolding of CFTR Domains In Vivo." Molecular Biology of the Cell 20, no. 7 (April 2009): 1903–15. http://dx.doi.org/10.1091/mbc.e08-09-0950.

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The cystic fibrosis transmembrane conductance regulator (CFTR) architecture consists of two membrane spanning domains (MSD1 and -2), two nucleotide binding domains (NBD1 and -2), and a regulatory (R) domain. Several point mutations lead to the channel misprocessing, with limited structural perturbation of the mutant domain. To gain more insight into the basis of CFTR folding defect, the contribution of domain-wise and cooperative domain folding was assessed by determining 1) the minimal domain combination that is recognized as native and can efficiently escape the endoplasmic reticulum (ER) retention and 2) the impact of mutation on the conformational coupling among domains. One-, two-, three-, and most of the four-domain assemblies were retained at the ER. Solubilization mutations, however, rescued the NBD1 processing defect conceivably by thermodynamic stabilization. The smallest folding unit that traversed the secretory pathway was composed of MSD1-NBD1-R-MSD2 as a linear or split polypeptide. Cystic fibrosis-causing missense mutations in the MSD1, NBD1, MSD2, and NBD2 caused conformational defect in multiple domains. We propose that cooperative posttranslational folding is required for domain stabilization and provides a plausible explanation for the global misfolding caused by point mutations dispersed along the full-length CFTR.

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Wannebroucq, Amélie, Guillaume Gruntz, Jean-Moïse Suisse, Yohann Nicolas, Rita Meunier-Prest, Mickaël Mateos, Thierry Toupance, and Marcel Bouvet. "New n-type molecular semiconductor–doped insulator (MSDI) heterojunctions combining a triphenodioxazine (TPDO) and the lutetium bisphthalocyanine (LuPc2) for ammonia sensing." Sensors and Actuators B: Chemical 255 (February 2018): 1694–700. http://dx.doi.org/10.1016/j.snb.2017.08.184.

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RAMJEESINGH, Mohabir, Francisca UGWU, Canhui LI, Sonja DHANI, Ling Jun HUAN, Yanchun WANG, and Christine E. BEAR. "Stable dimeric assembly of the second membrane-spanning domain of CFTR (cystic fibrosis transmembrane conductance regulator) reconstitutes a chloride-selective pore." Biochemical Journal 375, no. 3 (November 1, 2003): 633–41. http://dx.doi.org/10.1042/bj20030774.

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Structural information is required to define the molecular basis for chloride conduction through CFTR (cystic fibrosis transmembrane conductance regulator). Towards this goal, we expressed MSD2, the second of the two MSDs (membrane-spanning domains) of CFTR, encompassing residues 857–1158 in Sf9 cells using the baculovirus system. In Sf9 plasma membranes, MSD2 migrates as expected for a dimer in non-dissociative PAGE, and confers the appearance of an anion permeation pathway suggesting that dimeric MSD2 mediates anion flux. To assess directly the function and quaternary structure of MSD2, we purified it from Sf9 cells by virtue of its polyhistidine tag and nickel affinity. Reconstitution of MSD2 into liposomes conferred a 4,4′-di-isothiocyanostilbene-2,2′-disulphonate-inhibitable, chloride-selective electrodiffusion pathway. Further, this activity is probably mediated directly by MSD2 as reaction of its single cysteine residue (Cys866) with the thiol modifying reagent, Nα(3-maleimidylpropionyl)biocytin, inhibited chloride flux. Only MSD2 dimers were labelled by Nα(3-maleimidylpropionyl)biocytin, supporting the idea that only dimeric MSD2 can mediate anion flux. As a further test of this hypothesis, we conducted a second purification procedure, wherein purified dimeric and monomeric MSD2 proteins were reconstituted separately. Only proteoliposomes containing stable MSD2 dimers mediated chloride electrodiffusion, providing direct evidence that dimeric MSD2 mediates chloride channel function. In summary, we have shown that the second membrane domain of CFTR can be purified and functionally reconstituted as a chloride channel, providing a tool for probing the structural basis of chloride conduction through CFTR.

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Westlake, Christopher J., Susan P. C. Cole, and Roger G. Deeley. "Role of the NH2-terminal Membrane Spanning Domain of Multidrug Resistance Protein 1/ABCC1 in Protein Processing and Trafficking." Molecular Biology of the Cell 16, no. 5 (May 2005): 2483–92. http://dx.doi.org/10.1091/mbc.e04-12-1113.

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Multidrug resistance protein (MRP)1/ABCC1 transports organic anionic conjugates and confers resistance to cytotoxic xenobiotics. In addition to two membrane spanning domains (MSDs) typical of most ATP-binding cassette (ABC) transporters, MRP1 has a third MSD (MSD0) of unknown function. Unlike some topologically similar ABCC proteins, removal of MSD0 has minimal effect on function, nor does it prevent MRP1 from trafficking to basolateral membranes in polarized cells. However, we find that independent of cell type, the truncated protein accumulates in early/recycling endosomes. Using a real-time internalization assay, we demonstrate that MSD0 is important for MRP1 retention in, or recycling to, the plasma membrane. We also show that MSD0 traffics independently to the cell surface and promotes membrane localization of the core-region of MRP1 when the two protein fragments are coexpressed. Finally, we demonstrate that MSD0 becomes essential for trafficking of MRP1 when the COOH-terminal region of the protein is mutated. These studies demonstrate that MSD0 and the COOH-terminal region contain redundant trafficking signals, which only become essential when one or the other region is missing or is mutated. These data explain apparent differences in the trafficking requirement for MSD0 and the COOH-terminal region of MRP1 compared with other ABCC proteins.

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Gladman, Nicholas, Yinping Jiao, Young Koung Lee, Lifang Zhang, Ratan Chopra, Michael Regulski, Gloria Burow, et al. "Fertility of Pedicellate Spikelets in Sorghum Is Controlled by a Jasmonic Acid Regulatory Module." International Journal of Molecular Sciences 20, no. 19 (October 8, 2019): 4951. http://dx.doi.org/10.3390/ijms20194951.

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As in other cereal crops, the panicles of sorghum (Sorghum bicolor (L.) Moench) comprise two types of floral spikelets (grass flowers). Only sessile spikelets (SSs) are capable of producing viable grains, whereas pedicellate spikelets (PSs) cease development after initiation and eventually abort. Consequently, grain number per panicle (GNP) is lower than the total number of flowers produced per panicle. The mechanism underlying this differential fertility is not well understood. To investigate this issue, we isolated a series of ethyl methane sulfonate (EMS)-induced multiseeded (msd) mutants that result in full spikelet fertility, effectively doubling GNP. Previously, we showed that MSD1 is a TCP (Teosinte branched/Cycloidea/PCF) transcription factor that regulates jasmonic acid (JA) biosynthesis, and ultimately floral sex organ development. Here, we show that MSD2 encodes a lipoxygenase (LOX) that catalyzes the first committed step of JA biosynthesis. Further, we demonstrate that MSD1 binds to the promoters of MSD2 and other JA pathway genes. Together, these results show that a JA-induced module regulates sorghum panicle development and spikelet fertility. The findings advance our understanding of inflorescence development and could lead to new strategies for increasing GNP and grain yield in sorghum and other cereal crops.

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ZHANG, Jian-Ting. "Determinant of the extracellular location of the N-terminus of human multidrug-resistance-associated protein." Biochemical Journal 348, no. 3 (June 7, 2000): 597–606. http://dx.doi.org/10.1042/bj3480597.

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Multidrug-resistance-associated protein (MRP) is a member of the ATP-binding cassette (ABC) membrane-transport superfamily and is responsible for multidrug resistance in cancer cells. Distinct from other members of the ABC superfamily, MRP has three membrane-spanning domains (MSDs) and the N-terminus is located extracellularly. It has been shown that the first MSD (MSD1) with an extracellular N-terminus is important for MRP function. To address what ensures the generation of this structural organization of MRP and to understand in general the molecular mechanism of membrane folding of polytopic proteins with extracellular N-termini, the biogenesis of MSD1 in human MRP1 was examined using an in vitro expression system. Surprisingly, the second transmembrane segment (TM2) in MSD1 was found to play a critical role in the correct membrane translocation and folding of MSD1 in human MRP1. TM2 not only plays an essential role to ensure the N-terminus-outside/C-terminus-inside orientation of TM1 with an extracellular N-terminus, it can also translocate into membranes post-translationally in a signal-recognition particle and ribosome-dependent manner to provide an additional insurance for correct folding of MSD1 in MRP. These findings suggest that TM2 in a polytopic membrane protein with an extracellular N-terminus may play a critical role in controlling correct membrane translocation and folding of the protein in general.

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Lambe, J., P. Cadby, and M. Gibney. "Comparison of stochastic modelling of the intakes of intentionally added flavouring substances with theoretical added maximum daily intakes (TAMDI) and maximized survey-derived daily intakes (MSDI)." Food Additives and Contaminants 19, no. 1 (January 2002): 2–14. http://dx.doi.org/10.1080/02652030110071327.

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Slot, Andrew J., Steven V. Molinski, and Susan P. C. Cole. "Mammalian multidrug-resistance proteins (MRPs)." Essays in Biochemistry 50 (September 7, 2011): 179–207. http://dx.doi.org/10.1042/bse0500179.

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Subfamily C of the human ABC (ATP-binding cassette) superfamily contains nine proteins that are often referred to as the MRPs (multidrug-resistance proteins). The ‘short’ MRP/ABCC transporters (MRP4, MRP5, MRP8 and ABCC12) have a typical ABC structure with four domains comprising two membrane-spanning domains (MSD1 and MSD2) each followed by a nucleotide-binding domain (NBD1 and NBD2). The ‘long’ MRP/ABCCs (MRP1, MRP2, MRP3, ABCC6 and MRP7) have five domains with the extra domain, MSD0, at the N-terminus. The proteins encoded by the ABCC6 and ABCC12 genes are not known to transport drugs and are therefore referred to as ABCC6 and ABCC12 (rather than MRP6 and MRP9) respectively. A large number of molecules are transported across the plasma membrane by the MRPs. Many are organic anions derived from exogenous sources such as conjugated drug metabolites. Others are endogenous metabolites such as the cysteinyl leukotrienes and prostaglandins which have important signalling functions in the cell. Some MRPs share a degree of overlap in substrate specificity (at least in vitro), but differences in transport kinetics are often substantial. In some cases, the in vivo substrates for some MRPs have been discovered aided by studies in gene-knockout mice. However, the molecules that are transported in vivo by others, including MRP5, MRP7, ABCC6 and ABCC12, still remain unknown. Important differences in the tissue distribution of the MRPs and their membrane localization (apical in contrast with basolateral) in polarized cells also exist. Together, these differences are responsible for the unique pharmacological and physiological functions of each of the nine ABCC transporters known as the MRPs.

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Daoud, Roni, Michel Julien, Philippe Gros, and Elias Georges. "Major Photoaffinity Drug Binding Sites in Multidrug Resistance Protein 1 (MRP1) Are within Transmembrane Domains 10–11 and 16–17." Journal of Biological Chemistry 276, no. 15 (January 4, 2001): 12324–30. http://dx.doi.org/10.1074/jbc.m009782200.

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MRP1 is an ABC (or ATP binding cassette) membrane transport protein shown to confer resistance to structurally dissimilar drugs. Studies of MRP1 topology suggested the presence of a hydrophobic N-domain with five potential membrane-spanning domains linked to an MDR1-like core (MSD1-NBD1-L1-MSD2-NBD2) by an intracellular linker domain (L0). MRP1-mediated multidrug resistance is thought to be due to enhanced drug efflux. However, little is known about MRP1-drug interaction and its drug binding site(s). We previously developed several photoreactive probes to study MRP1-drug interactions. In this report, we have used eight MRP1-HA variants that were modified to have hemagglutinin A (HA) epitopes inserted at different sites in MRP1 sequence. Exhaustive in-gel digestion of all IAARh123 photoaffinity-labeled MRP1-HA variants revealed the same profile of photolabeled peptides as seen for wild type MRP1. Photolabeling of the different MRP1-HA variants followed by digestion with increasing concentrations of trypsin orStaphylococcus aureusV8 protease (1:800 to 1:5 w/w) and immunoprecipitation with anti-HA mAb identified two small photolabeled peptides (∼6–7 kDa) from MRP1-HA(574) and MRP1-HA(1222). Based on the location of the HA epitopes in the latter variants together with molecular masses of the two peptides, the photolabeled amino acid residues were localized to MRP1 sequences encoding transmembranes 10 and 11 of MSD1 (Ser542-Arg593) and transmembranes 16 and 17 of MSD2 (Cys1205-Glu1253). Interestingly, the same sequences in MRP1 were also photolabeled with a structurally different photoreactive drug, IACI, confirming the significance of transmembranes 10, 11, 16 and 17 in MRP1 drug binding. Taken together, the results in this study provide the first delineation of the drug binding site(s) of MRP1. Furthermore, our findings suggest the presence of common drug binding site(s) for structurally dissimilar drugs.

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Grove, Diane E., Meredith F. N. Rosser, Hong Yu Ren, Anjaparavanda P. Naren, and Douglas M. Cyr. "Mechanisms for Rescue of Correctable Folding Defects in CFTRΔF508." Molecular Biology of the Cell 20, no. 18 (September 15, 2009): 4059–69. http://dx.doi.org/10.1091/mbc.e08-09-0929.

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Premature degradation of CFTRΔF508 causes cystic fibrosis (CF). CFTRΔF508 folding defects are conditional and folding correctors are being developed as CF therapeutics. How the cellular environment impacts CFTRΔF508 folding efficiency and the identity of CFTRΔF508's correctable folding defects is unclear. We report that inactivation of the RMA1 or CHIP ubiquitin ligase permits a pool of CFTRΔF508 to escape the endoplasmic reticulum. Combined RMA1 or CHIP inactivation and Corr-4a treatment enhanced CFTRΔF508 folding to 3–7-fold greater levels than those elicited by Corr-4a. Some, but not all, folding defects in CFTRΔF508 are correctable. CHIP and RMA1 recognize different regions of CFTR and a large pool of nascent CFTRΔF508 is ubiquitinated by RMA1 before Corr-4a action. RMA1 recognizes defects in CFTRΔF508 related to misassembly of a complex that contains MSD1, NBD1, and the R-domain. Corr-4a acts on CFTRΔF508 after MSD2 synthesis and was ineffective at rescue of ΔF508 dependent folding defects in amino-terminal regions. In contrast, misfolding caused by the rare CF-causing mutation V232D in MSD1 was highly correctable by Corr-4a. Overall, correction of folding defects recognized by RMA1 and/or global modulation of ER quality control has the potential to increase CFTRΔF508 folding and provide a therapeutic approach for CF.

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Villani, Sara M., Jon Hulvey, Jean-Michel Hily, and Kerik D. Cox. "Overexpression of the CYP51A1 Gene and Repeated Elements are Associated with Differential Sensitivity to DMI Fungicides in Venturia inaequalis." Phytopathology® 106, no. 6 (June 2016): 562–71. http://dx.doi.org/10.1094/phyto-10-15-0254-r.

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The involvement of overexpression of the CYP51A1 gene in Venturia inaequalis was investigated for isolates exhibiting differential sensitivity to the triazole demethylation inhibitor (DMI) fungicides myclobutanil and difenoconazole. Relative expression (RE) of the CYP51A1 gene was significantly greater (P < 0.0001) for isolates with resistance to both fungicides (MRDR phenotype) or with resistance to difenoconazole only (MSDR phenotype) compared with isolates that were resistant only to myclobutanil (MRDS phenotype) or sensitive to both fungicides (MSDS phenotype). An average of 9- and 13-fold increases in CYP51A1 RE were observed in isolates resistant to difenoconazole compared with isolates with MRDS and MSDS phenotypes, respectively. Linear regression analysis between isolate relative growth on myclobutanil-amended medium and log10 RE revealed that little to no variability in sensitivity to myclobutanil could be explained by CYP51A1 overexpression (R2 = 0.078). To investigate CYP51A1 upstream anomalies associated with CYP51A1 overexpression or resistance to difenoconazole, Illumina sequencing was conducted for three isolates with resistance to difenoconazole and one baseline isolate. A repeated element, “EL 3,1,2”, with the properties of a transcriptional enhancer was identified two to four times upstream of CYP51A1 in difenoconazole-resistant isolates but was not found in isolates with the MRDS phenotype. These results suggest that different mechanisms may govern resistance to different DMI fungicides in the triazole group.

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Smith, Emma E., Gwenaëlle Conseil, and Susan P. C. Cole. "Conserved amino acids in the region connecting membrane spanning domain 1 to nucleotide binding domain 1 are essential for expression of the MRP1 (ABCC1) transporter." PLOS ONE 16, no. 2 (February 11, 2021): e0246727. http://dx.doi.org/10.1371/journal.pone.0246727.

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Multidrug resistance protein 1 (MRP1) (gene symbol ABCC1) is an ATP-binding cassette (ABC) transporter which effluxes xeno- and endobiotic organic anions including estradiol glucuronide and the pro-inflammatory leukotriene C4. MRP1 also confers multidrug resistance by reducing intracellular drug accumulation through active efflux. MRP1 has three membrane spanning domains (MSD), and two nucleotide binding domains (NBD). MSD1 and MSD2 are linked to NBD1 and NBD2 by connecting regions (CR) 1 and CR2, respectively. Here we targeted four residues in CR1 (Ser612, Arg615, His622, Glu624) for alanine substitution and unexpectedly, found that cellular levels of three mutants (S612A, R615A, E624A) in transfected HEK cells were substantially lower than wild-type MRP1. Whereas CR1-H622A properly trafficked to the plasma membrane and exhibited organic anion transport activity comparable to wild-type MRP1, the poorly expressing R615A and E624A (and to a lesser extent S612A) mutant proteins were retained intracellularly. Analyses of cryogenic electron microscopic and atomic homology models of MRP1 indicated that Arg615 and Glu624 might participate in bonding interactions with nearby residues to stabilize expression of the transporter. However, this was not supported by double exchange mutations E624K/K406E, R615D/D430R and R615F/F619R which failed to improve MRP1 levels. Nevertheless, these experiments revealed that the highly conserved CR1-Phe619 and distal Lys406 in the first cytoplasmic loop of MSD1 are also essential for expression of MRP1 protein. This study is the first to demonstrate that CR1 contains several highly conserved residues critical for plasma membrane expression of MRP1 but thus far, currently available structures and models do not provide any insights into the underlying mechanism(s). Additional structures with rigorous biochemical validation data are needed to fully understand the bonding interactions critical to stable expression of this clinically important ABC transporter.

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Wang, Chanfei, Jiai He, and Haiyan Chen. "Decision-Feedback Aided Multiple-Symbol Differential Detection in Two-Way Relay Transmission." Mathematical Problems in Engineering 2019 (July 18, 2019): 1–7. http://dx.doi.org/10.1155/2019/8595091.

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In this paper, noncoherent transmission algorithms are proposed in a two-way relay transmission (TWRT), where differential space-time block codes based multiple-symbol differential detection (MSDD) is performed. Specifically, generalized likelihood ratio test aided MSDD (GLRT-MSDD) is developed with the exhaustive search in the TWRT. In order to solve the challenging problem of high complexity, the GLRT-MSDD model is reformulated and a decision-feedback aided MSDD (DF-MSDD) model is derived. Furthermore, performance analysis and the simulations confirm that the proposed DF-MSDD provides solid bit error-rate performance with a lower complexity than GLRT-MSDD in the TWRT.

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RAVIKUMAR, BALA. "THE BENFORD-NEWCOMB DISTRIBUTION AND UNAMBIGUOUS CONTEXT-FREE LANGUAGES." International Journal of Foundations of Computer Science 19, no. 03 (June 2008): 717–27. http://dx.doi.org/10.1142/s0129054108005905.

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For a string w ∈ {0,1, 2,…, d-1}*, let vald(w) denote the integer whose base d representation is the string w and let MSDd(x) denote the most significant or the leading digit of a positive integer x when x is written as a base d integer. Hirvensalo and Karhumäki [9] studied the problem of computing the leading digit in the ternary representation of 2x ans showed that this problem has a polynomial time algorithm. In [16], some applications are presented for the problem of computing the leading digit of AB for given inputs A and B. In this paper, we study this problem from a formal language point of view. Formally, we consider the language Lb,d,j = {w|w ∈ {0,1, 2,…, d-1}*, 1 ≤ j ≤ 9, MSDb(dvalb(w))) = j} (and some related classes of languages) and address the question of whether this and some related languages are context-free. Standard pumping lemma proofs seem to be very difficult for these languages. We present a unified and simple combinatorial technique that shows that these languages are not unambiguous context-free languages. The Benford-Newcomb distribution plays a central role in our proofs.

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Villamil Giraldo, Ana-Maria, Ida Eriksson, Stefan Wennmalm, Timmy Fyrner, Thomas Ederth, and Karin Öllinger. "Interactions of the Lysosomotropic Detergent O-Methyl-Serine Dodecylamide Hydrochloride (MSDH) with Lipid Bilayer Membranes—Implications for Cell Toxicity." International Journal of Molecular Sciences 21, no. 9 (April 29, 2020): 3136. http://dx.doi.org/10.3390/ijms21093136.

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O-methyl-serine dodecylamine hydrochloride (MSDH) is a detergent that accumulates selectively in lysosomes, a so-called lysosomotropic detergent, with unexpected chemical properties. At physiological pH, it spontaneously forms vesicles, which disassemble into small aggregates (probably micelles) below pH 6.4. In this study, we characterize the interaction between MSDH and liposomes at different pH and correlate the findings to toxicity in human fibroblasts. We find that the effect of MSDH on lipid membranes is highly pH-dependent. At neutral pH, the partitioning of MSDH into the liposome membrane is immediate and causes the leakage of small fluorophores, unless the ratio between MSDH and lipids is kept low. At pH 5, the partitioning of MSDH into the membrane is kinetically impeded since MSDH is charged and a high ratio between MSDH and the lipids is required to permeabilize the membrane. When transferred to cell culture conditions, the ratio between MSDH and plasma membrane lipids must therefore be low, at physiological pH, to maintain plasma membrane integrity. Transmission electron microscopy suggests that MSDH vesicles are taken up by endocytosis. As the pH of the endosomal compartment progressively drops, MSDH vesicles disassemble, leading to a high concentration of increasingly charged MSDH in small aggregates inside the lysosomes. At sufficiently high MSDH concentrations, the lysosome is permeabilized, the proteolytic content released to the cytosol and apoptotic cell death is induced.

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Tubagus, Ade P., Diana V. D. Doda, and Herlina I. S. Wungouw. "Hubungan Tingkat Risiko Musculoskeletal Disorders (MSDs) Mengguna-kan Rapid Entire Body Assessment (REBA) dengan Keluhan MSDs pada Residen Ilmu Bedah." JURNAL BIOMEDIK (JBM) 10, no. 3 (December 18, 2018): 168. http://dx.doi.org/10.35790/jbm.10.3.2018.21982.

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Abstract: According to WHO, musculoskeletal disorders are categorized as the second rank of work-related disease. Various factors such as work, individual, and social factors can cause MSDs. These disorders play an important role in morbidity of workers such as healthcare workers. This study was aimed to analyze the correlation between the risk level of MSDs evaluated by using REBA and MSDs complaints among surgery residents. This was an analytical observational study with a cross-sectional design. The instruments used in this study were the Rapid Entire Body Assessment (REBA) worksheet and the Nordic Body Map (NBM) questionnaire. Data were analyzed with the Spearman correlation test. There were 42 surgery residents of Faculty of Medicine, Sam Ratulangi University, Manado participated in this study. The majority of them were males (81%) and young adults (88%). The prevalence of MSDs complaints were as follows: 60% of respondents had mild complaints, 33% had moderate complaints, and 7% had severe complaints. The bivariate analysis showed that the risk level of MSDs evaluated by REBA had a strong positive correlation with MSDs complaints (P=0.000; r=0.603). Conclusion: There was a significant correlation between risk level of MSDs evaluated by using REBA and the MSDs complaints. Based on the results, ergonomics interventions are recommended to surgery residents in order to prevent the occurence of MSDs.Keywords: musculoskeletal disorders (MSDs), REBA, surgery residentsAbstrak: Musculoskeletal disorders (MSDs) menurut WHO berada di urutan kedua terbanyak penyakit akibat kerja. Berbagai faktor seperti pekerjaan serta faktor individu dan sosial dapat menyebabkan terjadinya MSDs. Gangguan ini berperan dalam morbiditas di banyak bidang pekerjaan salah satunya di bidang kesehatan. Penelitian ini bertujuan untuk mengetahui hubungan tingkat risiko MSDs menggunakan REBA dengan keluhan MSDs pada residen ilmu bedah. Jenis penelitan ialah analitik observasional dengan desain potong lintang. Alat ukur yang digunakan ialah lembar kerja Rapid Entire Body Assessment (REBA) dan kuesioner Nordic Body Map (NBM). Analisis data menggunakan uji korelasi Spearman. Hasil penelitian mendapatkan sebanyak 42 residen ilmu bedah Fakultas Kedokteran Universitas Sam Ratulangi Manado menjadi responden penelitian. Mayoritas responden berjenis kelamin laki-laki (81%) dan berada dalam kategori dewasa muda (88%). Prevalensi keluhan MSDs ialah 60% keluhan MSDs ringan, 33% keluhan sedang, dan 7% keluhan berat. Hasil uji korelasi Spearman mendapatkan tingkat risiko MSDs menggunakan REBA memiliki hubungan positif kuat dengan keluhan MSDs (P=0,000, r=0,603). Simpulan: Terdapat hubungan bermakna antara tingkat risiko MSDs menggunakan REBA dengan keluhan MSDs. Berdasarkan hasil penelitian maka direkomendasikan intervensi ergonomi pada residen bedah untuk mencegah terjadinya keluhan MSDs.Kata kunci: musculoskeletal disorders (MSDs), REBA, residen bedah

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Jang, Hye Young, Kyoung Won Kim, So Yeon Kim, Jin Sil Kim, Sang Hyun Choi, Se-Young Kim, and Sung-Gyu Lee. "Visibility of the graft hepatic artery using superb microvascular imaging in liver transplantation recipients: initial experience." Acta Radiologica 59, no. 11 (February 10, 2018): 1326–35. http://dx.doi.org/10.1177/0284185118757275.

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Background Hepatic artery (HA) obstruction is one of the most threatening complications following liver transplantation (LT); however, conventional color Doppler imaging (CDI) suffers from technical limitations regarding the visualization of fine vessels and low-velocity blood flow. Purpose To test the visibility of HA in postoperative evaluation of LT using a superb microvascular imaging (SMI). Material and Methods This retrospective study was approved by our institutional review board. Fifty-five consecutive patients (58 grafts; mean age = 56 years) who underwent LT with Doppler ultrasonography (US) on postoperative day 1 were included. We compared the subjective visibility of HA and objective measurements of HA caliber, visible HA length on CDI, monochrome SMI (mSMI), contrast-enhanced mSMI (CE-mSMI), and contrast harmonic imaging (CHI). Reproducibility of HA caliber measurements on SMI techniques were also evaluated by using intraclass correlation coefficients (ICCs). Results The subjective image quality for visibility of HA tended to be graded higher with mSMI than CDI, and with CE-mSMI than mSMI ( P < 0.001). The overall reproducibility of HA caliber measurements was good to excellent for both mSMI and CE-mSMI (ICC = 0.674–0.855). HA caliber measurements on mSMI and CE-mSMI strongly correlated with CHI ( R = 0.785, 0.798, P < 0.001), while mean HA length on mSMI was significantly longer than on CDI (1.88 ± 0.83 vs. 1.42 ± 1.01cm, P = 0.004), and even longer on CE-mSMI (vs. 3.28 ± 1.11 cm, P < 0.001). Conclusion The mSMI technique shows good reproducibility and correlates well with currently used methods for postoperative evaluation of HA in LT recipients. It is further improved by administration of an US contrast agent.

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Journal articles on the topic 'MSDI'

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