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1

Pulgarín-R, Paulo, Martha Olivera-Angel, Luisa Ortíz, Duván Nanclares, Sara Velásquez-Restrepo, and Juan Díaz-Nieto. "DNA barcodes of birds from northern Colombia." Biodiversity Data Journal 9 (May 21, 2021): e64842. https://doi.org/10.3897/BDJ.9.e64842.

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DNA barcode datasets are a useful tool for conservation and aid in taxonomic identification, particularly in megadiverse tropical countries seeking to document and describe its biota, which is dropping at an alarming rate during recent decades. Here we report the barcodes for several low elevation bird species from northern Colombia with the goal to provide tools for species identification in this region of South America. We blood-sampled birds in a lowland tropical forest with various degrees of intervention using standard 3 × 12 m mist-nets. We extracted DNA and sequenced the COI barcode gen
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Ståhls, Gunilla. "Pelecocera (Pelecocera) tricincta and Pelecocera (Chamaesyrphus) caledonica (Diptera, Syrphidae) reared from Rhizopogon fungal host in Finland." Biodiversity Data Journal 12 (April 15, 2024): e118563. https://doi.org/10.3897/BDJ.12.e118563.

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MtDNA COI barcodes have frequently been used in identification to associate an unknown life stage in insects with a known species. This study reports the discovery of hoverfly larvae in the fungal fruit bodies of <i>Rhizopogon luteolus</i> Fr. &amp; Nordholm, 1817 in Finland. The identity of the larvae was firstly resolved using mtDNA COI barcodes generated from the larvae and tree-based identification confirming the species Pelecocera (Pelecocera) tricincta Meigen, 1822 and Pelecocera (Chamaesyrphus) caledonica (Collin, 1940) (Diptera, Syrphidae). Obtained pupae were reared into adult flies a
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3

Papadopoulou, Anna, Johannes Bergsten, Tomochika Fujisawa, Michael T. Monaghan, Timothy G. Barraclough, and Alfried P. Vogler. "Speciation and DNA barcodes: testing the effects of dispersal on the formation of discrete sequence clusters." Philosophical Transactions of the Royal Society B: Biological Sciences 363, no. 1506 (2008): 2987–96. http://dx.doi.org/10.1098/rstb.2008.0066.

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Large-scale sequencing of short mtDNA fragments for biodiversity inventories (‘DNA barcoding’) indicates that sequence variation in animal mtDNA is highly structured and partitioned into discrete genetic clusters that correspond broadly to species-level entities. Here we explore how the migration rate, an important demographic parameter that is directly related to population isolation, might affect variation in the strength of mtDNA clustering among taxa. Patterns of mtDNA variation were investigated in two groups of beetles that both contain lineages occupying habitats predicted to select for
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4

van Nieukerken, Erik J., Camiel Doorenweerd, Frank R. Stokvis та Dick S. J. Groenenberg. "DNA barcoding of the leaf-mining moth subgenus Ectoedemia s. str. (Lepidoptera: Nepticulidae) with COI and EF1-α: two are better than one in recognising cryptic species". Contributions to Zoology 81, № 1 (2012): 1–24. http://dx.doi.org/10.1163/18759866-08101001.

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We sequenced 665bp of the Cytochrome C Oxidase I (COI) barcoding marker for 257 specimens and 482bp of Elongation Factor 1-α (EF1-α) for 237 specimens belonging to the leafmining subgenus Ectoedemia (Ectoedemia) in the basal Lepidopteran family Nepticulidae. The dataset includes 45 out of 48 West Palearctic Ectoedemia s. str. species and several species from Africa, North America and Asia. Both COI and EF1-α proved reliable as an alternative to conventional species identification for the majority of species and the combination of both markers can aid in species validation. A clear barcode gap
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5

Hardy, Christopher M., Mark Adams, Dean R. Jerry, Leon N. Court, Matthew J. Morgan, and Diana M. Hartley. "DNA barcoding to support conservation: species identification, genetic structure and biogeography of fishes in the Murray - Darling River Basin, Australia." Marine and Freshwater Research 62, no. 8 (2011): 887. http://dx.doi.org/10.1071/mf11027.

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Freshwater fish stocks worldwide are under increasing threat of overfishing, disease, pollution and competition from introduced species. In the Murray—Darling Basin (MDB), the largest river system of Australia, more than half the native species are listed as rare or endangered. Active management is required to counteract reduction in population sizes, prevent local extinctions and to maintain genetic diversity. We describe the first comprehensive set of DNA barcodes able to discriminate between all 58 native and introduced species of freshwater fish recorded in the MDB. These barcodes also dis
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6

Duran, Daniel P., Robert A. Laroche, Harlan M. Gough, et al. "Geographic Life History Differences Predict Genomic Divergence Better than Mitochondrial Barcodes or Phenotype." Genes 11, no. 3 (2020): 265. http://dx.doi.org/10.3390/genes11030265.

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Species diversity can be inferred using multiple data types, however, results based on genetic data can be at odds with patterns of phenotypic variation. Tiger beetles of the Cicindelidia politula (LeConte, 1875) species complex have been taxonomically problematic due to extreme phenotypic variation within and between populations. To better understand the biology and taxonomy of this group, we used mtDNA genealogies and multilocus nuclear analyses of 34,921 SNPs to elucidate its evolutionary history and evaluate the validity of phenotypically circumscribed species and subspecies. Genetic analy
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7

Zilli, Alberto, Gyula M. László, Anthony J. Kingston, and Knud Larsen. "A peculiar new species of <i>Nyodes</i> Laporte, 1970 (Lepidoptera: Noctuidae)." Metamorphosis 34, no. 1 (2023): 97–104. http://dx.doi.org/10.4314/met.v34i1.8.

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A new, externally atypical Nyodes is described from Equatorial Africa: Nyodes paludicola sp. nov. The new species is compared to the superficially most similar Nyodes steelei Laporte, 1971, and N. punctatoides Laporte, 1973, with the closest genital morphology. The new species is associated with wetland habitats and is widely distributed in West, Central and East Africa. Genetic divergence between the geographically distant populations has been assessed based on mtDNA COI barcodes.
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8

Savolainen, Eino. "Baetis jaervii sp. n. (Ephemeroptera: Baetidae) from northern Europe." Entomologica Fennica 20, no. 3 (2019): 182–85. http://dx.doi.org/10.33338/ef.84475.

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Baetis jaervii sp. n. is described and illustrated. The history and relationships of this taxon to related species are elucidated on the basis of literature and determinations of genetic distance and mtDNA barcodes. B. jaervii is the only representative of the B. vernus group found in northern Europe, the nymphs of which live in lakes. The population from northern Finland earlier assigned to Baetis tracheatus should be transferred to represent B. jaervii.
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9

Zhang, Ao, Wenbing Liu, and Shaowei Qiu. "Mitochondrial genetic variations in leukemia: a comprehensive overview." Blood Science 6, no. 4 (2024): e00205. http://dx.doi.org/10.1097/bs9.0000000000000205.

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Leukemias are a group of heterogeneous hematological malignancies driven by diverse genetic variations, and the advent of genomic sequencing technologies facilitates the investigation of genetic abnormalities in leukemia. However, these sequencing-based studies mainly focus on nuclear DNAs. Increasing evidence indicates that mitochondrial dysfunction is an important mechanism of leukemia pathogenesis, which is closely related to the mitochondrial genome variations. Here, we provide an overview of current research progress concerning mitochondrial genetic variations in leukemia, encompassing ge
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10

Combosch, David J., David Burdick, Karim Primov, Dareon Rios, Kireon Rios, and Jessica Fernandez. "Barcoding and mitochondrial phylogenetics of Porites corals." PLOS ONE 19, no. 2 (2024): e0290505. http://dx.doi.org/10.1371/journal.pone.0290505.

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Coral reefs are the most diverse ecosystem on the planet based on the abundance and diversity of phyla and higher taxa. However, it is still difficult to assess the diversity of lower taxa, especially at the species level. One tool for improving the identification of lower taxa are genetic markers that can distinguish cryptic species and assess species boundaries. Here, we present one such approach for an important and challenging group of reef-building corals. Porites corals are the main reef-builders of many coral reefs in the Indo-Pacific, owing to the massive growth forms of some species.
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11

Ngui, Romano, Veeranoot Nissapatorn, and Guo-Jie Brandon-Mong. "Prospects on the Application of DNA Barcoding on Soil-Transmitted Helminths in Children." Journal of Pediatric Infectious Diseases 12, no. 04 (2017): 256–63. http://dx.doi.org/10.1055/s-0037-1603561.

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AbstractSince more than a decade, DNA barcoding has been widely used to examine biological samples and differentiate species, as well as employed in ecological and conservational studies. There is a growing interest of DNA barcoding, particularly in medical parasitology, but its potential utility in soil-transmitted helminths (STHs) remains unclear. Therefore, in this article, we review the studies using DNA barcoding and its applications in medical parasitology with special focus on STHs such as Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus, and Trichuris trichiura. DNA barc
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12

Pelingen, Arthien Lovell, and Hendrik Freitag. "Description of Neoperla mindoroensis sp. nov., the first record of a stonefly from Mindoro, Philippines (Plecoptera, Perlidae), and identification of its life stages using COI barcodes." ZooKeys 954 (July 29, 2020): 47–63. http://dx.doi.org/10.3897/zookeys.954.53746.

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The new stonefly species, Neoperla mindoroensissp. nov. (Perlidae), from Mindoro island is described. The new species is assigned to the N. recta species complex of the N. montivaga group on account of its obvious T7 and T8 with pointed processes and the presence of basolateral lobes in the everted aedeagal sac. The male adult is distinguishable by its aedeagus with a slightly raised mediodorsal lobe, fully covered with fine spinules, while the female adult has comparably small eggs (240 × 220 μm) with a punctate, chorionic surface with punctae arranged in polygonal FCIs. The life stages and s
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13

Ståhls, Gunilla, and Eino Savolainen. "MtDNA COI barcodes reveal cryptic diversity in the Baetis vernus group (Ephemeroptera, Baetidae)." Molecular Phylogenetics and Evolution 46, no. 1 (2008): 82–87. http://dx.doi.org/10.1016/j.ympev.2007.09.009.

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14

Pelingen, Arthien Lovell, and Hendrik Freitag. "Description of Neoperla mindoroensis sp. nov., the first record of a stonefly from Mindoro, Philippines (Plecoptera, Perlidae), and identification of its life stages using COI barcodes." ZooKeys 954 (July 29, 2020): 47–63. https://doi.org/10.3897/zookeys.954.53746.

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The new stonefly species, Neoperla mindoroensis sp. nov. (Perlidae), from Mindoro island is described. The new species is assigned to the N. recta species complex of the N. montivaga group on account of its obvious T7 and T8 with pointed processes and the presence of basolateral lobes in the everted aedeagal sac. The male adult is distinguishable by its aedeagus with a slightly raised mediodorsal lobe, fully covered with fine spinules, while the female adult has comparably small eggs (240 × 220 μm) with a punctate, chorionic surface with punctae arranged in polygonal FCIs. The life stages and
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15

Kodada, Ján, Manfred A. Jäch, Hendrik Freitag, et al. "Ancyronyx clisteri, a new spider riffle beetle species from Borneo, redescription of A. sarawacensis Jäch including a description of the larva and new distribution data for A. procerus Jäch using DNA barcodes (Coleoptera, Elmidae)." ZooKeys 912 (February 17, 2020): 25–64. http://dx.doi.org/10.3897/zookeys.912.47796.

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Ancyronyx clisterisp. nov. (Coleoptera, Elmidae) a new spider riffle beetle discovered from northern Borneo (Brunei; Sabah and Sarawak, Malaysia) and the larva of Ancyronyx sarawacensis Jäch are described. Illustrations of the habitus and diagnostic characters of the new species and the similar and highly variable A. sarawacensis are presented. Differences to closely related species, based on DNA barcodes and morphological characters, are discussed. Association of the larva and the imago of A. sarawacensis, and the occurrence of Ancyronyx procerus Jäch in Peninsular Malaysia and Sabah are conf
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16

Pešić, Vladimir, Andrzej Zawal, Aleksandra Bańkowska, Milica Jovanović, and Miroslawa Dabert. "A new crenobiontic water mite species of the genus Atractides Koch, 1837 from Montenegro and Bulgaria, based on morphological and molecular data (Acariformes, Hydrachnidia, Hygrobatidae)." Systematic and Applied Acarology 25, no. 10 (2020): 1889–900. http://dx.doi.org/10.11158/saa.25.10.12.

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A new species of water mites from Montenegro and Bulgaria, Atractides anae Pešić sp. nov., is described, combining traditional morphological techniques with the analysis of partial mtDNA COI sequences (DNA-barcodes). Based on our molecular and morphological analysis, the new species is most closely related to the crenobiontic A. pennatus (K. Viets 1920) from which it differs by 6.4 (SD = 1.12)% K2P distance. A dataset including the novel COI sequences of 10 Atractides species from Montenegro and Iran is assembled in order to facilitate further studies on the genus by using molecular tools.
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17

Poorani, J., S. K. Jalali, and Rakshit Ojha. "Morphological characters and DNA barcodes to separate Oenopia sauzeti Mulsant and O. mimica Weise (Coleoptera: Coccinellidae), two externally similar lady beetles from the Indian subcontinent." ENTOMON 40, no. 4 (2015): 235–42. http://dx.doi.org/10.33307/entomon.v40i4.98.

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Oenopia sauzeti Mulsant and O. mimica Weise (Coleoptera: Coccinellidae) are externally very similar and commonly misidentified species of lady beetles distributed in the Indian subcontinent. Diagnostic characters including male genitalia are illustrated for these species to facilitate their identification. The cox1 mtDNA sequences of O. sauzeti and O. mimica (658 bp) had only 89% similarity upon pair-wise alignment, which distinguished them with 75 nucleotide differences, thus confirming that these are distinct species. DNA barcodes with accession numbers AGIMP042-15 for O. sauzeti and AGIMP04
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18

Kodada, Ján, Manfred A. Jäch, Hendrik Freitag, et al. "Ancyronyx clisteri , a new spider riffle beetle species from Borneo, redescription of A. sarawacensis Jäch including a description of the larva and new distribution data for A. procerus Jäch using DNA barcodes (Coleoptera, Elmidae)." ZooKeys 912 (February 17, 2020): 25–64. https://doi.org/10.3897/zookeys.912.47796.

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Ancyronyx clisteri sp. nov. (Coleoptera, Elmidae) a new spider riffle beetle discovered from northern Borneo (Brunei; Sabah and Sarawak, Malaysia) and the larva of Ancyronyx sarawacensis Jäch are described. Illustrations of the habitus and diagnostic characters of the new species and the similar and highly variable A. sarawacensis are presented. Differences to closely related species, based on DNA barcodes and morphological characters, are discussed. Association of the larva and the imago of A. sarawacensis, and the occurrence of Ancyronyx procerus Jäch in Peninsular Malaysia and Sabah are con
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19

Thu, Pham The, Nguyen Manh Linh, Nguyen Van Quan, Pham Van Chien, Dao Huong Ly, and Le Ba Hoang Hiep. "DNA barcoding for identification of some fish species (Carangidae) in Vietnam coastal area." Tạp chí Khoa học và Công nghệ biển 19, no. 4 (2020): 527–36. http://dx.doi.org/10.15625/1859-3097/19/4/14909.

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Carangidae family has got about 148 species belonging to 32 genera. In Vietnam, Carangidae is of high commercial value and playing an important role in the ecosystem. In the context Vietnam has received yellow card for seafood since Nov. 2017 by the EU, in which one of the main reasons was related to the restriction of traceability. In this study, DNA barcoding technique of mitochondrial cytochrome oxidase I (COI) gene was used to classify 56 specimens of Carangidae from three coastal areas (Northern, Central and Southern) in Vietnam to evaluate the effectiveness compared to the morphological
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20

Whitworth, T. L., R. D. Dawson, H. Magalon, and E. Baudry. "DNA barcoding cannot reliably identify species of the blowfly genus Protocalliphora (Diptera: Calliphoridae)." Proceedings of the Royal Society B: Biological Sciences 274, no. 1619 (2007): 1731–39. http://dx.doi.org/10.1098/rspb.2007.0062.

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In DNA barcoding, a short standardized DNA sequence is used to assign unknown individuals to species and aid in the discovery of new species. A fragment of the mitochondrial gene cytochrome c oxidase subunit 1 is emerging as the standard barcode region for animals. However, patterns of mitochondrial variability can be confounded by the spread of maternally transmitted bacteria that cosegregate with mitochondria. Here, we investigated the performance of barcoding in a sample comprising 12 species of the blow fly genus Protocalliphora , known to be infected with the endosymbiotic bacteria Wolbac
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21

Solovyeva, Evgeniya N., Evgeniy A. Dunayev, Roman A. Nazarov, Dmitriy A. Bondarenko, and Nikolay A. Poyarkov. "COI-Barcoding and Species Delimitation Assessment of Toad-Headed Agamas of the Genus Phrynocephalus (Agamidae, Squamata) Reveal Unrecognized Diversity in Central Eurasia." Diversity 15, no. 2 (2023): 149. http://dx.doi.org/10.3390/d15020149.

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We provide a diversity assessment of the agamid genus Phrynocephalus Kaup, 1825. We analyze COI mtDNA barcodes from 385 individuals sampled all over Phrynocephalus range. We apply the ABGD, ASAP, bGMYC, mlPTP and hsPTP species delimitation algorithms to analyze the COI gene fragment variation and assess the species diversity in Phrynocephalus. Nine species groups are revealed in Phrynocephalus in agreement with earlier studies on the phylogenetic relationships of the genus. We demonstrate that the present taxonomy likely underestimates the actual diversity of the genus. Alternative species del
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22

Schmidt, B. Christian, and Ross A. Layberry. "What Azure blues occur in Canada? A re-assessment of Celastrina Tutt species (Lepidoptera, Lycaenidae)." ZooKeys 584 (April 26, 2016): 135–64. https://doi.org/10.3897/zookeys.584.7882.

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The identity of Celastrina species in eastern Canada is reviewed based on larval host plants, phenology, adult phenotypes, mtDNA barcodes and re-assessment of published data. The status of the Cherry Gall Azure (C. serotina Pavulaan &amp; Wright) as a distinct species in Canada is not supported by any dataset, and is removed from the Canadian fauna. Previous records of this taxon are re-identified as C. lucia (Kirby) and C. neglecta (Edwards). Evidence is presented that both Celastrina lucia and Celastrina neglecta have a second, summer-flying generation in parts of Canada. The summer generati
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23

Lohse, Konrad. "Can mtDNA Barcodes Be Used to Delimit Species? A Response to Pons et al. (2006)." Systematic Biology 58, no. 4 (2009): 439–42. http://dx.doi.org/10.1093/sysbio/syp039.

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Nazari, Vazrick, and Wolfgang ten Hagen. "Molecular taxonomy of Tomares hairstreaks (Lepidoptera, Lycaenidae, Theclinae)." Deutsche Entomologische Zeitschrift 67, no. 1 (2020): 19–33. http://dx.doi.org/10.3897/dez.67.50252.

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Tomares hairstreaks comprise about 10 species distributed from Europe and North Africa to Central Asia. The taxonomy of the genus is hampered by the absence of diagnostic characters by which specimens can be unambiguously assigned to species. Our investigation of morphology and DNA barcode variations within and between Tomares species shows that while well-defined species (T. ballus, T. mauritanicus, T. callimachus, T. desinens and T. fedtschenkoi) diverge, poorly characterized taxa (T. nogelii, T. nesimachus, T. dobrogensis, T. romanovi and T. telemachus) show very little to no differentiatio
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Nazari, Vazrick, and Hagen Wolfgang ten. "Molecular taxonomy of Tomares hairstreaks (Lepidoptera, Lycaenidae, Theclinae)." Deutsche Entomologische Zeitschrift 67, no. (1) (2020): 19–33. https://doi.org/10.3897/dez.67.50252.

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Tomares hairstreaks comprise about 10 species distributed from Europe and North Africa to Central Asia. The taxonomy of the genus is hampered by the absence of diagnostic characters by which specimens can be unambiguously assigned to species. Our investigation of morphology and DNA barcode variations within and between Tomares species shows that while well-defined species (T. ballus, T. mauritanicus, T. callimachus, T. desinens and T. fedtschenkoi) diverge, poorly characterized taxa (T. nogelii, T. nesimachus, T. dobrogensis, T. romanovi and T. telemachus) show very little to no differentiatio
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Ardura, Alba, Serge Planes, and Eva Garcia-Vazquez. "Applications of DNA barcoding to fish landings: authentication and diversity assessmente." ZooKeys 365 (December 30, 2013): 49–65. https://doi.org/10.3897/zookeys.365.6409.

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DNA barcoding methodologies are being increasingly applied not only for scientific purposes but also for diverse real-life uses. Fisheries assessment is a potential niche for DNA barcoding, which serves for species authentication and may also be used for estimating within-population genetic diversity of exploited fish. Analysis of single-sequence barcodes has been proposed as a shortcut for measuring diversity in addition to the original purpose of species identification. Here we explore the relative utility of different mitochondrial sequences (12S rDNA, COI, Cytb, and D-Loop) for application
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MOORE, MICHAEL D., ETHAN P. BEAVER, ALEJANDRO VELASCO-CASTRILLÓN, and MARK I. STEVENS. "Two new endemic species of Abantiades Herrich-Schäffer (Lepidoptera: Hepialidae) from Kangaroo Island, Australia." Zootaxa 4951, no. 3 (2021): 571–97. http://dx.doi.org/10.11646/zootaxa.4951.3.9.

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Abantiades penneshawensis Moore &amp; Beaver sp. nov. and Abantiades rubrus Moore &amp; Beaver sp. nov. are described as new. Both species are endemic to Kangaroo Island, and although both are related to species that occur on the Australian mainland and other islands, they are distinguished from those sister and phenotypically similar species by morphology and mtDNA (COI) barcodes. These two new species raise the number of Abantiades species on Kangaroo Island to six, three being endemic, and 45 species in the genus for the whole of Australia. There are now 13 species of Hepialidae (one undesc
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ČIAMPOR, FEDOR Jr, ZUZANA ČIAMPOROVÁ-ZAŤOVIČOVÁ, and JÁN KODADA. "A new species of Neblinagena Spangler from Kukenán tepui and DNA barcoding of Neblinagena and related genera (Coleoptera: Elmidae)." Zootaxa 4286, no. 2 (2017): 176. http://dx.doi.org/10.11646/zootaxa.4286.2.2.

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We describe here a new species of the genus Neblinagena Spangler, 1985 from the foothills of the Kukenán tepui (Venezuela). Neblinagena mira sp. n. occurs sympatrically with Neblinagena doylei Kodada &amp; Jäch, 1999, from which it differs mainly in the shape of thorax, shorter setae around eyes, pointed apices of elytra and shape of the male genitalia. We describe also the larva of the new species, which is the first description of a larva in the genus. Larva was assigned to the adults of N. mira sp. n. using mtDNA barcodes. Molecular data were also used to estimate relationships of the new s
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Sharkey, Michael J., Austin Baker, Kathryn McCluskey, et al. "Minimalist revision of Mesochorus Gravenhorst, 1829 (Hymenoptera: Ichneumonidae: Mesochorinae) from Área de Conservación Guanacaste, Costa Rica, with 158 new species and host records for 129 species." Revista de Biología Tropical 71, S2 (2023): 1–174. http://dx.doi.org/10.15517/rev.biol.trop..v71is2.56316.

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Introduction: Species of Mesochorus are found worldwide and members of this genus are primarily hyperparasitoids of Ichneumonoidea and Tachinidae. &#x0D; Objectives: To describe species of Costa Rican Mesochorus reared from caterpillars and to a lesser extent Malaise-trapped. &#x0D; Methods: The species are diagnosed by COI mtDNA barcodes, morphological inspection, and host data. A suite of images and host data (plant, caterpillar, and primary parasitoid) are provided for each species.&#x0D; Results: A total of 158 new species of Mesochorus. Sharkey is the taxonomic authority for all. &#x0D; C
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Breugelmans, Karin, Kurt Jordaens, Els Adriaens, Jean Paul Remon, Cardona Josep Quintana, and Thierry Backeljau. "DNA barcodes and phylogenetic affinities of the terrestrial slugs Arion gilvus and A. ponsi (Gastropoda, Pulmonata, Arionidae)." ZooKeys 365 (December 30, 2013): 83–104. https://doi.org/10.3897/zookeys.365.6104.

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The Iberian Peninsula is a region with a high endemicity of species of the terrestrial slug subgenus <i>Mesarion</i>. Many of these species have been described mainly on subtle differences in their proximal genitalia. It therefore remains to be investigated 1) whether these locally diverged taxa also represent different species under a phylogenetic species concept as has been shown for other <i>Mesarion</i> species outside the Iberian Peninsula, and 2) how these taxa are phylogenetically related. Here, we analysed DNA sequence data of two mitochondrial (COI and 16S) genes, and of the nuclear I
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Ušanović, Lejla, Jasna Hanjalić, Semir Dorić, et al. "CURRENT PERSPECTIVES OF DNA BARCODING IN BOSNIA AND HERZEGOVINA THROUGH BOLD DATABASE." Radovi Šumarskog fakulteta Univerziteta u Sarajevu 50, no. 1 (2021): 33–37. http://dx.doi.org/10.54652/rsf.2020.v50.i1.40.

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UDK: 577.212:574]:007.5(497.6)&#x0D; DNA barcoding is a method designed to provide rapid and precise species identifications by using one or more of short gene sequences called barcodes. In most plant and fungi studies, the standard barcodes of choice are three plastid (rbcL, matK and trnH-psbA) and one nuclear (ITS) gene regions. The relatively high, but comparatively conserved rate of sequence evolution of mtDNA has made COI the marker of choice in animals. BOLD is a freely available cloud-based data storage and analysis platform developed with the aim to advance biodiversity science through
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MOORE, MICHAEL D., ETHAN P. BEAVER, ALEJANDRO VELASCO-CASTRILLÓN, and MARK I. STEVENS. "Description of two new Australian species of Abantiades Herrich-Schäffer (Lepidoptera: Hepialidae) and females of two further species with notes on their biogeography ." Zootaxa 4822, no. 1 (2020): 71–93. http://dx.doi.org/10.11646/zootaxa.4822.1.3.

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Abantiades cephalocorvus sp. nov. and Abantiades tembyi sp. nov. are described, along with the previously undescribed females of A. macropusinsulariae Simonsen, 2018 and A. pallida Simonsen, 2018. All of these species belong to a triforked Abantiades Herrich-Schäffer clade that is loosely centred around the Nullarbor and other arid regions of Australia. We explore DNA barcodes (mtDNA COI gene) from these and other Abantiades and discuss their significance for species recognition. The species distributions are entirely or largely allopatric and we discuss their origins from a widespread common
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33

Penter, Livius, Nicoletta Cieri, Katie Maurer, et al. "Integrative Single-Cell Tracking of Clonal Evolution, Leukemia Phenotypes and Donor Engraftment Following Allogeneic Stem Cell Transplant Using Mitochondrial DNA Mutations." Blood 142, Supplement 1 (2023): 3580. http://dx.doi.org/10.1182/blood-2023-180113.

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Mitochondrial DNA (mtDNA) mutations can distinguish cells from unrelated individuals and their spectrum per patient sample can evolve following therapeutic bottlenecks. As such, these natural barcodes can potentially enable integrated single cell tracking of chimeric cellular populations and clonal evolution following unrelated allogeneic hematopoietic stem cell transplantation (HSCT). However, the feasibility of mtDNA-based donor-recipient deconvolution or identification of leukemia-specific phenotypes and the extent of co-evolution with somatic mutations remains unknown. If confirmed, post-t
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34

Ceruso, Marina, Celestina Mascolo, Pasquale De Luca, et al. "A Rapid Method for the Identification of Fresh and Processed Pagellus erythrinus Species against Frauds." Foods 9, no. 10 (2020): 1397. http://dx.doi.org/10.3390/foods9101397.

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The commercialization of porgies or seabreams of the family Sparidae has greatly increased in the last decade, and some valuable species have become subject to seafood substitution. DNA regions currently used for fish species identification in fresh and processed products belong to the mitochondrial (mt) genes cytochrome b (Cytb), cytochrome c oxidase I (COI), 16S and 12S. However, these markers amplify for fragments with lower divergence within and between some species, failing to provide informative barcodes. We adopted comparative mitogenomics, through the analysis of complete mtDNA sequenc
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35

Martin, Melissa B., and Albert Chakona. "Designation of a neotype for Enteromius pallidus (Smith, 1841), an endemic cyprinid minnow from the Cape Fold Ecoregion, South Africa." ZooKeys 848 (May 20, 2019): 103–18. http://dx.doi.org/10.3897/zookeys.848.32211.

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Enteromiuspallidus was described by Smith in 1841 without a designated type specimen for the species. Herein, we designate a specimen from the Baakens River system as a neotype for E.pallidus and provide a thorough description for this species to facilitate ongoing taxonomic revisions of southern African Enteromius. Enteromiuspallidus can be distinguished from the other minnows in the “goldie barb group” by having an incomplete lateral line, lack of distinct chevron or tubular markings around lateral line pores, absence of a distinct lateral stripe, absence of wavy parallel lines along scale r
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36

RADENKOVIĆ, SNEŽANA, LAURA LIKOV, GUNILLA STÅHLS, et al. "Three new hoverfly species from Greece (Diptera: Syrphidae)." Zootaxa 4830, no. 1 (2020): 103–24. http://dx.doi.org/10.11646/zootaxa.4830.1.4.

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An ongoing investigation on the Greek hoverfly fauna using adult morphology has revealed new species within three genera. In this study, the knowledge of the Mediterranean hoverfly fauna has been enhanced by describing the following species: Cheilosia candida Vujić et Radenković sp. n. (Pindos Mountains), Paragus thracusi Radenković, Likov et Vujić sp. n. (Rhodope Mountains) and Psilota aegeae Vujić, Ståhls et Smit sp. n. (Lesvos island). Diagnosis of new species, as well as identification keys to the Mediterranean species of the subgenus Convocheila Barkalov of Cheilosia Meigen and the Europe
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37

BLAKEMORE, ROBERT J., TAE SEO PARK, and HONG-YUL SEO. "A new Korean earthworm (Oligochaeta: Megadrilacea: Megascolecidae)." Zootaxa 3368, no. 1 (2012): 256. http://dx.doi.org/10.11646/zootaxa.3368.1.13.

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Amynthas gageodo Blakemore, sp. nov. is described from small Gageo-do Island, offshore to the southwest of the Korean Peninsula in the Yellow Sea. It is an octothecal species (four pairs of spermathecae) comparable to Japanese Amynthas carnosus (Goto &amp; Hatai, 1899) (synonyms: Korean kyamikia Kobayashi, 1934, monstrifera Kobayashi, 1936, sangyeoli Hong &amp; James, 2001, youngtai Hong &amp; James, 2001, kimhaeiensis Hong &amp; James, 2001, sinsiensis Hong &amp; James, 2001, baemsagolensis Hong &amp; James, 2001, Taiwanese monsoonus James et al., 2005) and to Chinese A. pingi (Stephenson, 19
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38

Martin, Melissa B., and Albert Chakona. "Designation of a neotype for Enteromius pallidus (Smith, 1841), an endemic cyprinid minnow from the Cape Fold Ecoregion, South Africa." ZooKeys 848 (May 20, 2019): 103–18. https://doi.org/10.3897/zookeys.848.32211.

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Enteromius pallidus was described by Smith in 1841 without a designated type specimen for the species. Herein, we designate a specimen from the Baakens River system as a neotype for E. pallidus and provide a thorough description for this species to facilitate ongoing taxonomic revisions of southern African Enteromius. Enteromius pallidus can be distinguished from the other minnows in the "goldie barb group" by having an incomplete lateral line, lack of distinct chevron or tubular markings around lateral line pores, absence of a distinct lateral stripe, absence of wavy parallel lines along scal
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39

Leite, Luis Anderson Ribeiro. "Mitochondrial pseudogenes in insect DNA barcoding: differing points of view on the same issue." Biota Neotropica 12, no. 3 (2012): 301–8. http://dx.doi.org/10.1590/s1676-06032012000300029.

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Molecular tools have been used in taxonomy for the purpose of identification and classification of living organisms. Among these, a short sequence of the mitochondrial DNA, popularly known as DNA barcoding, has become very popular. However, the usefulness and dependability of DNA barcodes have been recently questioned because mitochondrial pseudogenes, non-functional copies of the mitochondrial DNA incorporated into the nuclear genome, have been found in various taxa. When these paralogous sequences are amplified together with the mitochondrial DNA, they may go unnoticed and end up being analy
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40

Penter, Livius, Nicoletta Cieri, Katie Maurer, et al. "Leukemia Evolution and Resistance to Graft-Versus-Leukemia Responses Revealed By Mitochondrial DNA Mutations." Blood 144, Supplement 1 (2024): 926. https://doi.org/10.1182/blood-2024-199009.

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Graft-versus-leukemia (GvL) responses enable successful allogeneic hematopoietic cell transplantation (HCT), a cornerstone treatment for high-risk blood cancers such as acute myeloid leukemia (AML) or advanced chronic lymphocytic leukemia (CLL). Yet, monitoring of GvL effects remains challenging, including early detection of emerging immune escape or tracking of clonal evolution during phases of incomplete leukemia control. We hypothesized that mitochondrial DNA (mtDNA) mutation analysis would be an effective tool for sensitive monitoring of clonal evolution and measurable residual disease (MR
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41

KAILA, LAURI, and GUNILLA STÅHLS. "DNA barcodes: Evaluating the potential of COI to diffentiate closely related species of Elachista (Lepidoptera: Gelechioidea: Elachistidae) from Australia." Zootaxa 1170, no. 1 (2006): 1. http://dx.doi.org/10.11646/zootaxa.1170.1.1.

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We compared DNA barcoding to “traditional” taxonomic tools in clarifying relationships in complexes of closely related, putative “species” of Elachistinae moths (Gelechioidea: Elachistidae) occurring in Australia. A 705 bp fragment of the 3’-end of cytochrome c oxidase subunit I gene (COI) was used. This mtDNA fragment did not differentiate between all species-level taxa that could be defined by morphological and/or ecological differences. Different evolutionary rates of COI among closely related lineages were observed. Although our findings are based on the variability of the 3’ end of the CO
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42

Huemer, Peter, and Jürg Schmid. "Relict populations of Lyonetia ledi Wocke, 1859 (Lepidoptera, Lyonetiidae) from the Alps indicate postglacial host-plant shift to the famous Alpenrose (Rhododendron ferrugineum L.)." Alpine Entomology 5 (November 22, 2021): 101–6. http://dx.doi.org/10.3897/alpento.5.76930.

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Lyonetia ledi Wocke, 1859 (Lyonetiidae), was hitherto considered as a boreal species with a circumpolar distribution pattern and relict populations in isolated peat bogs north-east of the Alps (Austria, Czech Republic, Germany). In Europe it is known as a leaf-miner on Rhododendron tomentosum Stokes ex Harmaja (Ericaceae) as the primary host-plant and also Myrica gale L. (Myricaceae). The first record of L. ledi from the Swiss Alps on Rhododendron ferrugineum L., the famous Alpenrose, indicates an ancient host-plant switch during postglacial periods when R. tomentosum and R. ferrugineum shared
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43

Couceiro, L., L. López, E. E. Sotka, J. M. Ruiz, and R. Barreiro. "Molecular data delineate cryptic Nassarius species and characterize spatial genetic structure of N. nitidus." Journal of the Marine Biological Association of the United Kingdom 92, no. 5 (2011): 1175–82. http://dx.doi.org/10.1017/s0025315411000816.

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Nassarius nitidus is a marine snail species with a widespread distribution along European shorelines from the North Sea to the Mediterranean and Black Seas. Despite its widespread distribution, N. nitidus has been largely neglected due to taxonomic confusion with the congeneric Nassarius reticulatus. Discrimination between these two Nassarius is particularly challenging in areas where their ranges overlap: the European Atlantic and western Mediterranean. Here, we propose the use of the mtDNA gene cytochrome c oxidase subunit I (COI) to discriminate between these two morphologically similar con
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44

Huemer, Peter, and Jürg Schmid. "Relict populations of Lyonetia ledi Wocke, 1859 (Lepidoptera, Lyonetiidae) from the Alps indicate postglacial host-plant shift to the famous Alpenrose (Rhododendron ferrugineum L.)." Alpine Entomology 5 (November 22, 2021): 101–6. https://doi.org/10.3897/alpento.5.76930.

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Lyonetia ledi Wocke, 1859 (Lyonetiidae), was hitherto considered as a boreal species with a circumpolar distribution pattern and relict populations in isolated peat bogs north-east of the Alps (Austria, Czech Republic, Germany). In Europe it is known as a leaf-miner on Rhododendron tomentosum Stokes ex Harmaja (Ericaceae) as the primary host-plant and also Myrica gale L. (Myricaceae). The first record of L. ledi from the Swiss Alps on Rhododendron ferrugineum L., the famous Alpenrose, indicates an ancient host-plant switch during postglacial periods when R. tomentosum and R. ferrugineum shared
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45

Blakemore, Robert, Seunghan Lee, Wonchoel Lee, and Hong-Yul Seo. "Two new Korean earthworms (Annelida, Oligochaeta, Megadrilacea, Megascolecidae)." ZooKeys 307 (June 6, 2013): 35–44. https://doi.org/10.3897/zookeys.307.5362.

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Two Korean endemic pheretimoid <i>Amynthas </i>Kinberg, 1867 species belonging in family Megascolecidae s. stricto are sketched, dissected and described. <i>Amynthas daeari </i>Blakemore sp. n. has spermathecae in 6/7/8 complying with an <i>A. tokioensis </i>spp-group, whilst <i>Amynthas jinburi </i>Blakemore sp. n. has spermathecal pores in 5 &amp; 6 strictly complying with Sims &amp; Easton’s (1972) <i>A. canaliculatus</i>-group. A definitive COI gene barcode is provided for the holotype of <i>A. daeari </i>but the age since collection or preservation of the <i>A. jinburi </i>type in 2000 pr
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46

Aguado-Aranda, Pablo, Antonio Ricarte, Zorica Nedeljković, et al. "Unveiling the Mainland vs. Insular Variability of the Eumerus barbarus Species Group (Diptera: Syrphidae) in the Western Mediterranean Basin." Insects 15, no. 4 (2024): 239. http://dx.doi.org/10.3390/insects15040239.

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Comprising nearly 300 described species, Eumerus Meigen, 1822, is one of the most speciose syrphid genera worldwide, and its taxonomic diversity is remarkable in the Mediterranean basin. The Eumerus barbarus (Coquebert, 1804) group consists of four species in the western Mediterranean. Although the phenotypic variability of this species group has been commented on in previous studies, it has never been contrasted with molecular data. In the present work, the morphological variation found in 300+ specimens of this species group from the western Mediterranean is explored and tested against the C
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47

GROLLMANN, MILENA MARIE, ASLAK JØRGENSEN, and NADJA MØBJERG. "Actinarctus doryphorus (Tanarctidae) DNA barcodes and phylogenetic reinvestigation of Arthrotardigrada with new A. doryphorus and Echiniscoididae sequences." Zootaxa 5284, no. 2 (2023): 351–63. http://dx.doi.org/10.11646/zootaxa.5284.2.7.

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Little is still known about the diversity and evolution of marine arthrotardigrades, as they are generally difficult to sample, resulting in a limited amount of molecular data for barcoding and phylogenetic studies. With the current study, we provide the first investigation into COI haplotype diversity in a marine tanarctid and at the same time readdress arthrotardigrade phylogeny. Specifically, we provide COI mtDNA, 18S and 28S rDNA sequences from a population of Actinarctus doryphorus (Tanarctidae) sampled off the coast of Roscoff, France and further provide new 18S sequences from two marine
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48

Jung, Sang Woo, Tatyana S. Vshivkova, and Yeon Jae Bae. "DNA-based identification of South Korean Megaloptera larvae with taxonomic notes." Canadian Entomologist 148, no. 2 (2015): 123–39. http://dx.doi.org/10.4039/tce.2015.44.

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AbstractMitochondrial DNA (mtDNA) sequences, which serve as DNA barcodes, have been used to associate immature and adult stages of insects and to delineate species. The partial mitochondrial cytochromecoxidase subunit I (COI) gene sequences for South Korean Megaloptera (all known in the adult stage) were tested to identify undetermined larvae as a rapid and effective method from 31 specimens (16 adults and 15 larvae). The COI gene sequences distinguished all six known Megaloptera species, with a low genetic distance between larvae and adults (0.50±0.21%). Based on the COI gene sequences, we as
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49

Blakemore, Robert. "Earthworms newly from Mongolia (Oligochaeta, Lumbricidae, Eisenia)." ZooKeys 285 (April 5, 2013): 1–21. https://doi.org/10.3897/zookeys.285.4502.

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Two new megadrile earthworms from the steppes, the first species wholly from Outer Mongolia, are ascribed to the partially parthenogenetic <em>Eisenia nordenskioldi</em> (Eisen, 1879) species-complex. Taxonomic justification of sympatric <em>Eisenia nordenskioldi</em> mongol and <em>E. nordenskioldi onon</em> <strong>ssp. n.</strong> are supported by mtDNA COI barcodes. The unreliability of molecular differentiation based on voucher names compared to definitive types is again demonstrated, as pertains to the ultimate <em>Eisenia andrei </em>Bouché, 1972 synonym of the <em>E. fetida </em>(Savig
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50

Stahls, Gunilla, and Anatolij V. Barkalov. "Taxonomic review of the Palaearctic species of the Cheilosia caerulescens-group (Diptera, Syrphidae)." ZooKeys 662 (March 22, 2017): 137–71. https://doi.org/10.3897/zookeys.662.11267.

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The Palaearctic species of the Cheilosia caerulescens group (Diptera: Syrphidae) are revised in this work. The species group belongs to the genus Cheilosia subgenus Taeniocheilosia Oldenberg. One new species is described from north Caucasus, Cheilosia (Taeniocheilosia) circassica sp. n. Cheilosia primulae Hering is established as a junior synonym of Cheilosia laeviventris Loew. Four lectotype designations are made. The species of the Cheilosia caerulescens group are redescribed and illustrated, and a table of diagnostic characters and an identification key to species are provided. MtDNA COI ba
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