Academic literature on the topic 'MTT assay test'

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Journal articles on the topic "MTT assay test"

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Kim, Kwang Mahn, Sang Bae Lee, Se Ho Lee, Yong Keun Lee, and Kyoung Nam Kim. "Comparison of Validity between WST-1 and MTT Test in Bioceramic Materials." Key Engineering Materials 284-286 (April 2005): 585–88. http://dx.doi.org/10.4028/www.scientific.net/kem.284-286.585.

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Cytotoxicity test was essential for the pre-clinical evaluation of bioceramics. Proliferation assays such as MTT, XTT and WST-1 were commonly used for measuring biocompatibility. WST-1 was more convenient than MTT because of its water-solubility and storage condition. The calcium phosphate glass and β-TCP have been used for bone substitute, and some magnetic ferrites have been used for hyperthermic treatment. L929, mouse fibroblast cell, was the representative cell-line for in vitro biocompatibility test. The extracts of test samples were prepared by ISO10993-12:2002. The biocompatibilities of
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Almutary, A., and B. J. S. Sanderson. "The MTT and Crystal Violet Assays." International Journal of Toxicology 35, no. 4 (2016): 454–62. http://dx.doi.org/10.1177/1091581816648906.

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The toxicological effects of nanoparticles (NPs) on humans, animals, and environment are largely unknown. Assessment of NPs cytotoxicity depends on the choice of the test system. Due to NPs optical activity and absorption values, they can influence the classical cytotoxicity assay. Eight NPs were spiked in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet assays and tested with HaCaT human skin cells. The MTT assay standard curve optical density (OD) measurements were altered by the presence of trisilanol phenyl and trisilanol isooctyl polyhedral oligome
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Kaushik, S. D., M. Vinchurkar, A. M. Patwardhan, et al. "MTT assay as a viability test of homograft valves." Indian Journal of Thoracic and Cardiovascular Surgery 11, no. 2 (1995): 38–41. http://dx.doi.org/10.1007/bf02860904.

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Kim, Mijoo, Marc Hayashi, Bo Yu, Thomas K. Lee, Reuben H. Kim, and Deuk-won Jo. "Cytotoxicity and Genotoxicity of Epoxy Resin-Based Root Canal Sealers before and after Setting Procedures." Life 12, no. 6 (2022): 847. http://dx.doi.org/10.3390/life12060847.

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Epoxy resin-based sealers are commonly used for successful endodontic treatment. This study aimed to evaluate the cytotoxicity and genotoxicity of epoxy resin-based sealers under unset and set conditions. Three epoxy resin-based sealers were used: Adseal, AH Plus, and Dia-Proseal. To test cytotoxicity, an agar overlay test and a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay were performed using unset and set sealers on L929 mouse fibroblasts. The genotoxicity test of the comet assay was performed using the same cell line. Extract dilutions in the culture media were
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M., Izdebska, Piątkowska-Chmiel I., Herbet M., Gawrońska-Grzywacz M., and Natorska-Chomicka D. "Evaluation of the Cytotoxic Effects of Combined Application of Methotrexate and Statins on Green Monkey Kidney Cells." International Journal of Clinical Pharmacology & Toxicology (IJCPT) 4, no. 5 (2015): 185–91. https://doi.org/10.19070/2167-910X-1500031.

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Lipid disorders that lead to cardiovascular diseases are also common with oncology patients. Therefore, this group of patients is exposed to the risk of interaction between the cytostatic drugs applied and statins. Methotrexate is widely used in cancer treatment. However, it is a highly toxic drug. Nephrotoxicity is the most common side effect following the methotrexate therapy. On the other hand, statins are reported to have possible renoprotective effects against drug-induced nephrotoxicity. The aim of this study was to evaluate the cytotoxic effects of the comb
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Chen, Cheng-Han, Yu-Ting Tsao, Po-Ting Yeh, et al. "Detection of Microorganisms in Body Fluids via MTT-PMS Assay." Diagnostics 12, no. 1 (2021): 46. http://dx.doi.org/10.3390/diagnostics12010046.

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Early detection of microorganisms is essential for the management of infectious diseases. However, this is challenging, as traditional culture methods are labor-intensive and time-consuming. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-phenazine methosulfate (MTT-PMS) assay has been used to evaluate the metabolic activity in live cells and can thus be used for detecting living microorganisms. With the addition of NaOH and Tris-EDTA, the same approach can be accelerated (within 15 min) and used for the quick detection of common bacterial pathogens. The assay results can be e
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Roguet, Roland, José Cotovio, Quintino Gaetani, Koovi G. Dossou, and André Rougier. "Cytotoxicity of 28 MEIC Chemicals to Rat Hepatocytes using Two Viability Endpoints: Correlation with Acute Toxicity Data in Rat and Man." Alternatives to Laboratory Animals 21, no. 2 (1993): 216–24. http://dx.doi.org/10.1177/026119299302100217.

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28 of the chemicals on the MEIC list were tested on rat hepatocytes in culture. Firstly, the metabolic capacities of the cell model were characterised, i.e. cytochrome P450 content and conjugation activities. Two independent endpoints, MTT (tetrazolium salt) reduction (for mitochondrial integrity) and neutral red uptake (NRU, for lysosomal integrity), were then used to measure the cytotoxicities of the test compounds after incubation with the cells for 24 hours. The relative toxicities of the test chemicals were measured by the determination of IC50 values for each parameter. Statistical analy
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Premamalini, Thayanidhi, Subramanian Anitha, Kanniappan Mohanapriya, and Anupma Jyoti Kindo. "Evaluation of 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide method for assessing biofilm formation in vitro by Trichosporon spp." Journal of Laboratory Physicians 10, no. 04 (2018): 380–86. http://dx.doi.org/10.4103/jlp.jlp_37_18.

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ABSTRACT BACKGROUND: Invasive infections due to Trichosporon spp. have increased recently and are frequently associated with indwelling medical devices. Such infections which are associated with biofilm formation do not respond to the routinely used antifungal agents and are often persistent, associated with high mortality rate. Various methods have been described by researchers to evaluate and quantify the biofilm formation. AIM: This study was conducted to compare two methods of biofilm production by Trichosporon sp, i.e., test tube method with crystal violet (CV) staining and 3-(4,5-dimethy
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Mshana, Robert N., Genet Tadesse, Getahun Abate, and Håkan Miörner. "Use of 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl Tetrazolium Bromide for Rapid Detection of Rifampin-ResistantMycobacterium tuberculosis." Journal of Clinical Microbiology 36, no. 5 (1998): 1214–19. http://dx.doi.org/10.1128/jcm.36.5.1214-1219.1998.

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We describe a test which uses the ability of viable cells to reduce 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to detect resistance to a bactericidal drug, rifampin, in in vitro-cultured Mycobacterium tuberculosis. The assay shows a linear relationship between the number of viable bacteria and the ability to reduce MTT. Dead mycobacteria were unable to reduce MTT. Rifampin-sensitive M. bovis (BCG) and M. tuberculosis exposed to rifampin showed a rifampin concentration-dependent inhibition of the ability to reduce MTT, while the resistant strains were unaffected. The in
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Vaibhav, Dhage* Shraddha Dhage Shraddha pokale varsha kurhe. "Assessment And MTT Examine OF Home-grown L-ascorbic acid Powder." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 3181–89. https://doi.org/10.5281/zenodo.13331548.

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The present work was focused on detailing advancement, assessment and MTT test of Home grown L-ascorbic acid Powder. The detailing was ready by blending Acerola Concentrate, Rosehip Separate, Moringa Concentrate, Amla Powder, Bilberry Concentrate, Glycine, L-lysine, L-proline, Elaichi Powder, Rock salt, Spirulina Powder and excipients, for example, Lemon flavor. At first, the pre detailing assessment of each different fixing was finished by assessing its Organoleptic characters, Mass thickness, Tap thickness, Hausner Proportion, Dampness Content, pH, and Strong state steadiness study. The deta
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Dissertations / Theses on the topic "MTT assay test"

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Boly, Raïnatou. "Caractérisation des propriétés anti-inflammatoires et anticancéreuses de la plante Agelanthus dodoneifolius (DC) Polh. & Wiens (Loranthaceae) utilisée en médecine traditionnelle au Burkina Faso." Doctoral thesis, Universite Libre de Bruxelles, 2012. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209769.

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Le présent travail a porté sur l’évaluation des propriétés anti-inflammatoires et anticancéreuses de Agelanthus dodoneifolius (Loranthaceae), communément appelée «gui africain». Cette plante hémiparasite est utilisée en médecine traditionnelle africaine pour le traitement de pathologies chroniques telles que l’asthme, l’hypertension, des gastroentérites et le cancer. Actuellement, les maladies chroniques représentent un problème mondial de santé publique. En effet, elles constituent la première cause de mortalité dans le monde surtout dans les pays à revenu faible ou intermédiaire. <p><p>Cette
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Ferdusová, Helena. "Syntéza kvantových teček pro in-vivo zobrazování." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-221307.

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The aim of this work was to synthesise water-soluble QDs using different precursors and stabilizers and to determine the toxicity of the synthesized QDs by in vivo imaging. Experiments were performed on water-soluble QDs (MPA-CdTe, MPA-CdTe/ZnS, MSA-CdTe, MSA-CdTe/ZnS, GSH-CdTe, GSH-CdTe/ZnS, TGA-CdTe, TGA-CdTe/ZnS, GSH-ZnSe and GSH-ZnSe/ZnS ) and toxicity was measured. Synthesized QDs were characterized by high intensity (fluorescence spectroscopy), FWHM and zeta potential (ZS Zetasizer) were selected due to their suitability for this task. The toxicity of QDs was determined by the MTT assay
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Watanabe, Simone [UNESP]. "Avaliação da biocompatibilidade e citotoxicidade dos cimentos: Endo-CPM-Sealer, Sealapex e Ângelus MTA." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/95448.

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Made available in DSpace on 2014-06-11T19:27:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-12-19Bitstream added on 2014-06-13T18:56:29Z : No. of bitstreams: 1 watanabe_s_me_araca.pdf: 310690 bytes, checksum: cd933b6e1170509831a34ff5afc29200 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>A necessidade de melhorar a qualidade dos materiais utilizados no tratamento endodôntico tem estimulado o estudo de vários materiais, buscando melhores condições de trabalho, biocompatibilidade e propriedade de estimular a ocorrência do selamento biológico. Este t
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Dhanjal, Perminder. "Investigation of the species differences and cytotoxic effects of coumarin and measurement of the cyctotoxic effects of various compounds in freshly isolated hepatocytes using several tests with particular interest in the MTT reduction assay freshly." Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281599.

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MAŠKOVÁ, Hana. "Antivirotické účinky stilbenoidů proti klíšťaty přenášeným patogenům in vivo." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-376087.

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This study was focused on antiviral effects of stilbenoids against a virus transmitted by ticks. The cell viability of selected cell line cultures in the presence of various concentrations of stilbenoids was determined using the MTT assay. Similarly, the mixed effect of other known antiviral substances and stilbenoids was studied using the MTT assay. Both the prophylactic effects of stilbenoids on the infected culture cell lines and the effect on viral replication were examined. The viral titres from samples were determined using plaque assay. Some of the experiments were performed also in viv
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Heinrich, Angelica [Verfasser]. "Syntheses and MTT assays of tetrathiafulvalene(TTF)-carbohydrate derivatives = Synthesen von Tetrathiafulvalen(TTF)-Kohlenhydratderivaten und deren Aktivitäten im MTT-Test / vorgelegt von Angelica Heinrich." 2003. http://d-nb.info/969656254/34.

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McCanna, David. "Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products." Thesis, 2009. http://hdl.handle.net/10012/4338.

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The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty associated with dose selection for clinical trials. The first section of this thesis describes how in vitro methods can be used to improve the prediction of the toxicity of chemicals and ophthalmic products. The proper utilization of in vitro methods can accurately predict toxic threshold levels and reduce animal use in product development. Sections two, three and four describe t
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Books on the topic "MTT assay test"

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R, Montesano, Griesemer R. A, Venitt S, International Program on Chemical Safety., and Commission of the European Communities., eds. Long-term and short-term assays for carcinogens: A critical appraisal : reports of an ad-hoc working group which met in Lyon, 2-6 Dec. 1985. International Agency for Research on Cancer, 1986.

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Comunicación rápida: Análisis moleculares como pruebas diagnósticas iniciales de la tuberculosis y la resistencia a la rifampicina. Organización Panamericana de la Salud, 2020. http://dx.doi.org/10.37774/9789275322383.

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Desde la aprobación por parte de la Organización Mundial de la Salud (OMS) de Xpert® MTB/RIF (Cepheid, Sunnyvale, EUA, en adelante denominado “Xpert MTB/RIF”) en el 2010, se ha generado un cúmulo considerable de evidencia sobre su uso como prueba diagnóstica inicial de la TB y la TB-RR. En los últimos meses también se han obtenido nuevos datos sobre el uso de Xpert® MTB/RIF Ultra (Cepheid, Sunnyvale, EUA, en adelante denominado “Xpert Ultra”) y sobre la última versión del sistema Truenat® MTB y MTB Plus (Molbio Diagnostics, Goa, India, en adelante denominado “Truenat”). La OMS encargó una revi
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Book chapters on the topic "MTT assay test"

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Londonkar, Ramesh, and Maithilee Kesralikar. "In Vitro Anticancer Screening of Methanolic Extract of Stachytarpheta Mutabilis." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022). Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_19.

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AbstractIn therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids,
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Cannizzaro, Francesco. "Gli errores (meta)letterari del Meandro da Augusto ai Flavi." In Studi e ricerche del Dipartimento di Lettere e Filosofia. Società Editrice Fiorentina, 2024. http://dx.doi.org/10.35948/dilef/978-88-6032-734-5.11.

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Il Meandro, fiume tortuoso per antonomasia tanto da dare il suo nome a un tipo di decorazione assai diffuso nell’arte greco-romana, in età augustea assume talora valore metaletterario sia come garanzia di epicità, a motivo del suo legame con il labirinto e l’antica Troia, sia viceversa come modello di una poetica “erratica”, metamorfica e vicina al polo dell’elegia. In questo contributo saranno brevemente analizzati e commentati in tal senso alcuni testi di Virgilio (Aen. V 250-251), Ovidio (met. VIII 159- 168; epist. IX 55-58) e Properzio (II 34, 33-40); sarà, infine, considerata la fortuna (
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Singhal, Mitali, Sabita Shaha, and Maria Katsikogianni. "Comparative Analysis of Cytotoxicity Assays, from Traditional to Modern Approaches." In Cytotoxicity - A Crucial Toxicity Test for In Vitro Experiments [Working Title]. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.1006842.

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This chapter will provide a comprehensive comparative analysis of various cytotoxicity assays, including traditional methods like the MTT assay and modern approaches such as the ATP assay and flow cytometry. Each method will be examined in detail, covering its principle, procedure, advantages, limitations, and specific applications in cytotoxicity testing. Additionally, the chapter will address the interconnectivity of different cell death pathways—apoptosis, autophagy, and necrosis—and discuss how various assays can be used in combination to achieve a more accurate and holistic assessment of
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Shobana, G., N. Agnel Arul John, G. Hariharan, and G. Sridharan. "In Vitro Antioxidant and In Vitro Cytotoxic Potentials of Ancardium occidentale Linn. Against Hep G2 Cell Lines." In Harnessing Medicinal Plants in Cancer Prevention and Treatment. IGI Global, 2024. http://dx.doi.org/10.4018/979-8-3693-1646-7.ch013.

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Cancer is a disease in which there is an uncontrolled multiplication and spread, within the body, of abnormal forms of the body's own cells. The Ethanol extract (EEAO) of Anacardium occidentale Linn was used for In-Vitro anticancer screening against HepG2 cell lines.Preliminary phytochemical analysis of the test drug was carried out and it revealed the presence of Tannin, Terpene, Quinone, Coumarin, Phenol. The results revealed that the extracts have significant Antioxidant potentials. In- vitro cytrotoxic assay such as tryphan blue dye exclusion, MTT and LDH releasing assays were carried out
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Mohammed, Mohsin, and Nadia Haj. "Preparation and Bioactivity Applications of Novel Chitosan Derivatives." In Chitin-Chitosan - Isolation, Properties, and Applications [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105796.

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Chitosan (CS) is a substance abundant in nature. It is a biopolymer consisting of repetitive components of glucose and N-acetyl-glucose amine connected by (1,4)-glycosidic bonds. It has so many applications that are biodegradable, non-toxic, and biocompatible. The CS was loaded with 5-fluorouracil (5FU) via amide-mediated binding, and the resulting CSFUAC product was evaluated as a potential 5FU delivery agent. A new CS-Schiff base derivative was created using CS extracted from local fish scales by combining CS with another aromatic aldehyde. The antimicrobial effectiveness of the new product
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Shivakumar, Prakruthi, and Kavitha Sunil Shettigar. "Tuberculosis Diagnosis: Updates and Challenges." In Mycobacterium - Epidemiology, Prevention, Diagnostic, and Management [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.107168.

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Tuberculosis (TB) is caused by a single infectious agent, Mycobacterium tuberculosis, and a public health concern due to increased cases of drug-resistance and high mortality rates. Rapid identification of tuberculosis is necessary for its early treatment and to prevent the emergence of drug-resistant strains. For effective management of patients, rapid, cost-effective, and point-of-care (POC) diagnostic methods are required. The commonly used screening and identification methods are clinical examination, radiography, sputum smear microscopy, culture method, serological method, and tuberculin
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Guneshwor, Kshetrimayum, and Adhikarimayum Haripyaree. "ASSESSMENT OF GENOTOXIC EFFECT OF PARIS POLYPHYLLA." In Futuristic Trends in Agriculture Engineering & Food Sciences Volume 3 Book 5. Iterative International Publisher, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bcag5p1ch13.

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The rhizome of Paris polyphylla Smith is used in number of health problems by the resident people of Manipur and connecting states. In the present study traditional chromosome aberration analysis, micronucleus assay and SC damages analysis are the three testing protocols of the cytogenetically assay to study whether aqueous extract of the plant interrelate with genetic material. Intraperitoneal injections of 3.6 ml/100g body weight (MTD) and 1.8 ml/100g body weight (less than MTD) of the aqueous extract to the animals have no significant higher frequencies. Animals received doses of distilled
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Chaumpluk, P. "DIY Lab-on-a-chip Platforms: A Solution for Simple Nucleic Acid-based Assays in the Absence of Proper (Chip) Facilities." In Lab-on-a-chip Devices for Advanced Biomedicines. Royal Society of Chemistry, 2024. http://dx.doi.org/10.1039/9781837673476-00362.

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Lab-on-a-chip technology plays a key role in nucleic acid-based assays due to its ability to overcome many issues affecting testing methods that depend heavily on a laboratory setting, which can often be time-consuming and lack the flexibility to perform tests on-site. Yet, a classical lab-on-a-chip system also involves some technical difficulties, instead relating to design, platform used, and mechanical control. Since nucleic acid assay depends on nucleic acid amplification and nucleic acid detection, the main technical issues associated with these areas in the context of chip fabrication ar
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Ustun Karatop, Eslin, Cagla Eren Cimenci, and Ayla Melisa Aksu. "Colorimetric Cytotoxicity Assays." In Cytotoxicity [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.105772.

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Cytotoxicity experiments are carried out to evaluate whether a chemical has cytotoxic potential. Because of its ease of use and compatibility with data collected from in vivo investigations, cell-based cytotoxicity studies have emerged as a viable alternative to animal trials in research. Cell-damaging events such as apoptosis, autophagy, and necrosis may occur after exposure to cytotoxic substances. Thanks to the cell-based cytotoxicity studies, basic information is obtained about the cytotoxic effects of the tested substance. To measure cell viability, a variety of techniques are used. Regar
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Sampath Kumar, L., K. Madhu Kumar, and P. Hariharan. "Green Synthesis of Calcium Carbonate-Zinc Oxide Nanocomposites (NPs) Methodology, Characterization, and Antimicrobial Property Evaluation." In Advances in Chemical and Materials Engineering. IGI Global, 2024. http://dx.doi.org/10.4018/979-8-3693-2798-2.ch010.

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This chapter presents an innovative approach to synthesizing calcium carbonate-zinc oxide (CaCO3-ZnO) nanocomposites using a green synthesis method. Utilizing silk fibroin wash water, this environmentally friendly technique enables the formation of stable nanocomposites without harmful chemicals. Detailed characterization confirmed the successful synthesis, composition, and structural properties of the CaCO3-ZnO nanocomposites. The antimicrobial activity tests demonstrated significant antibacterial activity against common pathogens. In the MTT assay, green synthesized NPs were administered at
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Conference papers on the topic "MTT assay test"

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Lapenko, A. K., E. R. Ol’hovaya, P. S. Kupcova, E. R. Lyapunova, and L. N. Komarova. "METHOD FOR ESTIMATING THE NUMBER OF VIABLE CELLS IN A CELL SPHEROID USING THE MTT ASSAY." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-27.

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One of the methods forstudying the viability of cell spheroids isthe MTT test. However, this test does not permit the determination of the number of viable cells within the spheroid at different stages of cultivation. Instead, it provides an indirect indication of the metabolic activity of cells. In this study, we propose a method for estimating the number of viable cells in a cell spheroid using the MTT test.
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Žegura, B., E. Heath, A. Černoša, and M. Filipič. "Toxicity and genotoxicity studies of surface and waste water samples using a bacterial SOS/umu test and mammalian MTT and comet assay." In ENVIRONMENTAL TOXICOLOGY 2006. WIT Press, 2006. http://dx.doi.org/10.2495/etox060161.

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Moreno, Marcelo, Daiane Manica, Gilnei Bruno da Silva, Ariane Zamoner, and Margarete Dulce Bagatini. "Antiproliferative effect of caffeine on a triple-negative breast cancer cell line." In XXVI Brazilian Mastology Congress. Mastology, 2024. https://doi.org/10.29289/259453942024v34s2034.

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Introduction: Among the main types of cancers leading to women's mortality, breast cancer is prominently highlighted. Specifically, triple-negative breast cancer (TNBC) is characterized by the lack of expression of progesterone, estrogen, and HER2 receptors, which limits the effectiveness of standard antineoplastic therapies since hormone therapy is ineffective, thereby increasing the risk of metastasis and recurrence. In this context, natural compounds are being studied for potential adjuvant use alongside traditional treatments. Methodology: Experiments were conducted in the cell culture lab
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Ahortor, E., S. Mahazu, T. Manful, A. Erber, and A. Ablordey. "Evaluation of an IS2404 LAMP protocol, a simple and rapid test for diagnosis of Buruli ulcer in low-resource settings." In MSF Scientific Days International 2024. MSF-USA, 2024. http://dx.doi.org/10.57740/mat4h7.

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INTRODUCTION Buruli ulcer caused by Mycobacterium ulcerans is a devastating necrotic skin disease. PCR, recommended for confirmation of Buruli ulcer by WHO, requires an adequately equipped laboratory, often delaying diagnosis and treatment of patients in remote or humanitarian settings. We aimed to assess loop- mediated isothermal amplification (LAMP), which is a molecular assay for isothermal amplification of DNA suggested for timely diagnosis of Buruli ulcer in low-resource settings. METHODS This study combines quantitative and qualitative methods. First, we evaluated a simple rapid syringe
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Scheefers-Borchel, U., and G. Muller-Berghaus. "A NEW FIBRIN-SPECIFIC ANTIBODY DISCRIMINATING BETWEEN FIBRIN AND FIBRINOGEN IS DIRECTED AGAINST THE SYNTHETIC PEPTIDE LEU-ILE-ASP-GLY-LYS-MET." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643771.

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To determine soluble fibrin in blood of patients with coagulation disorders we produced monoclonal antibodies which distinct fibrin from fibrinogen and other blood constituents. Fibrin-specific monoclonal antibodies were obtained by immunizing mice with the synthetic hexapeptide Leu-Ile-Asp-Gly-Lys-Met which was covalently linked to KLH via its C-terminus. Several of the monoclonal antibodies which reacted with the hexapeptide also reacted with batroxobin-induced desAA-fibrin and thrombin-induced desAABB-fibrin, but not with fibrinogen. No reaction was observed with plasmin-induced fibrinogeno
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Shital, Patil, Agarwal Kailash, and C. R. Choudhary. "Gene Xpert MTB/RIF assay in bronchoscopy guided bronchial wash specimens in sputum negative pulmonary tuberculosis: Sensitive, rapid and most useful test for drug sensitive and drug resistant tuberculosis." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa2773.

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Castro, SM, DPG Hubner, and LA Azevedo. "VALIDAÇÃO DO TESTE IGRA NO DIAGNÓSTICO DE INFECÇÃO LATENTE POR MYCOBACTERIUM TUBERCULOSIS EM UM LABORATÓRIO DA REDE PÚBLICA DE PORTO ALEGRE." In Resumos do 55º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s2.8261.

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Objetivo: A infecção latente pelo Mycobacterium tuberculosis (ILTB) é definida como um estado de resposta imune persistente à estimulação por antígenos de M. tuberculosis (MTB) sem sintomas clínicos ou achados radiológicos de doença ativa. A importância de diagnosticar a ILTB reside no potencial de reativação para a doença ativa e transmissível, quando o indivíduo estiver imunossuprimido com comorbidades ou em terapia biológica. Quando identificado o potencial de desenvolvimento de doença ativa em um indivíduo com ILTB, é recomendada a realização do tratamento preconizado, de forma a impedir q
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Cullen, John J., and Hugh L. MacIntyre. "The case for using the Most Probable Number (MPN) method in ballast water management system type approval testing." In IMarEST Ballast Water Technology Conference. IMarEST, 2017. http://dx.doi.org/10.24868/bwtc6.2017.010.

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Recently, the U.S. Coast Guard (USCG) rejected the Serial Dilution Culture-Most Probable Number (SDC-MPN) method for enumerating viable phytoplankton cells in ballast water discharge as an alternate to their prescribed method — the Environmental Technology Verification (ETV) Protocol. This method distinguishes living from dead organisms using vital stains and motility. Succinctly, the USCG position has been that the ETV Protocol is a reliable and repeatable efficacy test and the SDC-MPN method is not. New evidence and an expanded consideration of published research supports a fundamentally dif
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Reports on the topic "MTT assay test"

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เสรีมาศพันธุ์, อมรพันธุ์, จินตนา จิรถาวร, กนิษฐา ภัทรกุล та ธนาภัทร ปาละกะ. การพัฒนาชุดตรวจโรคเลปโตสไปโรซิสในรูปแผ่นตรวจโรคโดยใช้อนุภาคนาโนเมตร : รายงานการวิจัย. สำนักงานคณะกรรมการวิจัยแห่งชาติ, 2011. https://doi.org/10.58837/chula.res.2011.19.

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โรคเลปโตสไปโรซิสเป็นโรคติดเชื้อจากสัตว์สู่คนที่พบได้ทั่วโลก โดยมีเชื้อเลปโตสไปราสายพันธุ์ก่อโรคเป็นเชื้อสาเหตุ ปัจจุบันวิธีมาตรฐานที่ใช้ในการตรวจวินิจฉัยโรคเลปโตสไปโรซิสคือ Microscopic agglutination test (MAT) แต่วิธีนี้มีความไวไม่เพียงพอในการวินิจฉัยโรคในระยะต้นของการติดเชื้อ โปรตีน LipL32 เป็นโปรตีนที่พบมากที่สุดในผนังชั้นนอกของเชื้อเลปโตสไปราเฉพาะในสายพันธุ์ก่อโรคเท่านั้น นอกจากนั้นยังพบว่า โปรตีน LipL32 มีคุณสมบัติในการกระตุ้นภูมิคุ้มกันได้ดี และมีการแสดงออกระดับสูงในระยะต้นของการติดเชื้อ ดังนั้นโปรตีน LipL32 จึงเป็นแอนติเจนที่เหมาะสมสำหรับในการตรวจวินิจฉัยโรคเลปโตสไปโรซิสในระยะแรกเริ่ม กา
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Ruxrungtham, Kiat, Vorapot Sapsirisavat, Vorasit Vongsutilers, et al. Pharmaceutical equivalence drugs assessment-I (PEDA-I) : assess the pharmaceutical equivalence of generic antiretrovirals distributed in Thailand : Final report. Chulalongkorn University, 2016. https://doi.org/10.58837/chula.res.2016.30.

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Objectives: Ensuring that medicines meet quality standards is mandatory for ensuring safety and efficacy. There have been occasional reports of substandard generic medicines, especially in resource-limiting settings where policies to control quality may be less rigorous. As HIV treatment in Thailand depends mostly on affordable generic antiretrovirals (ARV), we performed quality assurance testing of several generic ARV available from different sources in Thailand and a source from Vietnam. Methods: We sampled Tenofovir 300 mg, Efavirenz 600 mg and Lopinavir/ritonavir 200/50mg from 10 primary h
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