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Journal articles on the topic 'MTT assay test'

Author: Grafiati

Published: 7 June 2025

Last updated: 16 July 2025

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1

Kim, Kwang Mahn, Sang Bae Lee, Se Ho Lee, Yong Keun Lee, and Kyoung Nam Kim. "Comparison of Validity between WST-1 and MTT Test in Bioceramic Materials." Key Engineering Materials 284-286 (April 2005): 585–88. http://dx.doi.org/10.4028/www.scientific.net/kem.284-286.585.

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Cytotoxicity test was essential for the pre-clinical evaluation of bioceramics. Proliferation assays such as MTT, XTT and WST-1 were commonly used for measuring biocompatibility. WST-1 was more convenient than MTT because of its water-solubility and storage condition. The calcium phosphate glass and β-TCP have been used for bone substitute, and some magnetic ferrites have been used for hyperthermic treatment. L929, mouse fibroblast cell, was the representative cell-line for in vitro biocompatibility test. The extracts of test samples were prepared by ISO10993-12:2002. The biocompatibilities of

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Almutary, A., and B. J. S. Sanderson. "The MTT and Crystal Violet Assays." International Journal of Toxicology 35, no. 4 (2016): 454–62. http://dx.doi.org/10.1177/1091581816648906.

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The toxicological effects of nanoparticles (NPs) on humans, animals, and environment are largely unknown. Assessment of NPs cytotoxicity depends on the choice of the test system. Due to NPs optical activity and absorption values, they can influence the classical cytotoxicity assay. Eight NPs were spiked in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet assays and tested with HaCaT human skin cells. The MTT assay standard curve optical density (OD) measurements were altered by the presence of trisilanol phenyl and trisilanol isooctyl polyhedral oligome

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Kaushik, S. D., M. Vinchurkar, A. M. Patwardhan, et al. "MTT assay as a viability test of homograft valves." Indian Journal of Thoracic and Cardiovascular Surgery 11, no. 2 (1995): 38–41. http://dx.doi.org/10.1007/bf02860904.

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Kim, Mijoo, Marc Hayashi, Bo Yu, Thomas K. Lee, Reuben H. Kim, and Deuk-won Jo. "Cytotoxicity and Genotoxicity of Epoxy Resin-Based Root Canal Sealers before and after Setting Procedures." Life 12, no. 6 (2022): 847. http://dx.doi.org/10.3390/life12060847.

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Epoxy resin-based sealers are commonly used for successful endodontic treatment. This study aimed to evaluate the cytotoxicity and genotoxicity of epoxy resin-based sealers under unset and set conditions. Three epoxy resin-based sealers were used: Adseal, AH Plus, and Dia-Proseal. To test cytotoxicity, an agar overlay test and a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay were performed using unset and set sealers on L929 mouse fibroblasts. The genotoxicity test of the comet assay was performed using the same cell line. Extract dilutions in the culture media were

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M., Izdebska, Piątkowska-Chmiel I., Herbet M., Gawrońska-Grzywacz M., and Natorska-Chomicka D. "Evaluation of the Cytotoxic Effects of Combined Application of Methotrexate and Statins on Green Monkey Kidney Cells." International Journal of Clinical Pharmacology & Toxicology (IJCPT) 4, no. 5 (2015): 185–91. https://doi.org/10.19070/2167-910X-1500031.

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Lipid disorders that lead to cardiovascular diseases are also common with oncology patients. Therefore, this group of patients is exposed to the risk of interaction between the cytostatic drugs applied and statins. Methotrexate is widely used in cancer treatment. However, it is a highly toxic drug. Nephrotoxicity is the most common side effect following the methotrexate therapy. On the other hand, statins are reported to have possible renoprotective effects against drug-induced nephrotoxicity. The aim of this study was to evaluate the cytotoxic effects of the comb

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Chen, Cheng-Han, Yu-Ting Tsao, Po-Ting Yeh, et al. "Detection of Microorganisms in Body Fluids via MTT-PMS Assay." Diagnostics 12, no. 1 (2021): 46. http://dx.doi.org/10.3390/diagnostics12010046.

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Early detection of microorganisms is essential for the management of infectious diseases. However, this is challenging, as traditional culture methods are labor-intensive and time-consuming. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-phenazine methosulfate (MTT-PMS) assay has been used to evaluate the metabolic activity in live cells and can thus be used for detecting living microorganisms. With the addition of NaOH and Tris-EDTA, the same approach can be accelerated (within 15 min) and used for the quick detection of common bacterial pathogens. The assay results can be e

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Roguet, Roland, José Cotovio, Quintino Gaetani, Koovi G. Dossou, and André Rougier. "Cytotoxicity of 28 MEIC Chemicals to Rat Hepatocytes using Two Viability Endpoints: Correlation with Acute Toxicity Data in Rat and Man." Alternatives to Laboratory Animals 21, no. 2 (1993): 216–24. http://dx.doi.org/10.1177/026119299302100217.

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28 of the chemicals on the MEIC list were tested on rat hepatocytes in culture. Firstly, the metabolic capacities of the cell model were characterised, i.e. cytochrome P450 content and conjugation activities. Two independent endpoints, MTT (tetrazolium salt) reduction (for mitochondrial integrity) and neutral red uptake (NRU, for lysosomal integrity), were then used to measure the cytotoxicities of the test compounds after incubation with the cells for 24 hours. The relative toxicities of the test chemicals were measured by the determination of IC50 values for each parameter. Statistical analy

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Premamalini, Thayanidhi, Subramanian Anitha, Kanniappan Mohanapriya, and Anupma Jyoti Kindo. "Evaluation of 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide method for assessing biofilm formation in vitro by Trichosporon spp." Journal of Laboratory Physicians 10, no. 04 (2018): 380–86. http://dx.doi.org/10.4103/jlp.jlp_37_18.

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ABSTRACT BACKGROUND: Invasive infections due to Trichosporon spp. have increased recently and are frequently associated with indwelling medical devices. Such infections which are associated with biofilm formation do not respond to the routinely used antifungal agents and are often persistent, associated with high mortality rate. Various methods have been described by researchers to evaluate and quantify the biofilm formation. AIM: This study was conducted to compare two methods of biofilm production by Trichosporon sp, i.e., test tube method with crystal violet (CV) staining and 3-(4,5-dimethy

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Mshana, Robert N., Genet Tadesse, Getahun Abate, and Håkan Miörner. "Use of 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl Tetrazolium Bromide for Rapid Detection of Rifampin-ResistantMycobacterium tuberculosis." Journal of Clinical Microbiology 36, no. 5 (1998): 1214–19. http://dx.doi.org/10.1128/jcm.36.5.1214-1219.1998.

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We describe a test which uses the ability of viable cells to reduce 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to detect resistance to a bactericidal drug, rifampin, in in vitro-cultured Mycobacterium tuberculosis. The assay shows a linear relationship between the number of viable bacteria and the ability to reduce MTT. Dead mycobacteria were unable to reduce MTT. Rifampin-sensitive M. bovis (BCG) and M. tuberculosis exposed to rifampin showed a rifampin concentration-dependent inhibition of the ability to reduce MTT, while the resistant strains were unaffected. The in

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Vaibhav, Dhage* Shraddha Dhage Shraddha pokale varsha kurhe. "Assessment And MTT Examine OF Home-grown L-ascorbic acid Powder." International Journal of Pharmaceutical Sciences 2, no. 8 (2024): 3181–89. https://doi.org/10.5281/zenodo.13331548.

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The present work was focused on detailing advancement, assessment and MTT test of Home grown L-ascorbic acid Powder. The detailing was ready by blending Acerola Concentrate, Rosehip Separate, Moringa Concentrate, Amla Powder, Bilberry Concentrate, Glycine, L-lysine, L-proline, Elaichi Powder, Rock salt, Spirulina Powder and excipients, for example, Lemon flavor. At first, the pre detailing assessment of each different fixing was finished by assessing its Organoleptic characters, Mass thickness, Tap thickness, Hausner Proportion, Dampness Content, pH, and Strong state steadiness study. The deta

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Clothier, Richard, Alex Orme, Tracey L. Walker, et al. "A Comparison of Three Cytotoxicity Endpoints in the Corneal HCE-T Model." Alternatives to Laboratory Animals 28, no. 2 (2000): 293–302. http://dx.doi.org/10.1177/026119290002800202.

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The prediction of ocular irritation potential from in vitro assays still presents a problem, despite a number of validation trials. A study with coded cosmetic formulations, for which historic in vivo data were available, has been conducted with a human corneal multilayered model system. This corneal model, the HCE-T model, was developed by using HCE-T cells, a transfected human corneal epithelial cell line. The relative effectiveness of three endpoints that provide a measure of cytotoxicity in the HCE-T model was evaluated. Cell viability immediately after exposure to the test materials was d

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Otoguro, Kazuhiko, Kanki Komiyama, Satoshi Ωmura, and Charles A. Tyson. "An In Vitro Cytotoxicity Assay Using Rat Hepatocytes and MTT and Coomassie Blue Dye as Indicators." Alternatives to Laboratory Animals 19, no. 3 (1991): 352–60. http://dx.doi.org/10.1177/026119299101900309.

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Isolated hepatocytes from male Sprague-Dawley rats suspended in culture medium supplemented with either 0.2 or 2% bovine serum albumin (BSA) were allowed to attach to collagen coated 96-well dishes. Ten test chemicals from the MEIC list and salicylic acid were added individually to the dishes, and at the end of 24 and 48 hours, cytotoxicity was determined by measuring MTT (tetrazolium salt) reduction (mitochondrial integrity) and total cellular protein using Coomassie blue dye (reflecting cell number). Total cellular lactate dehydrogenase activity was also determined in some experiments, as an

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TRITTEN, LUCIENNE, OLIVIER BRAISSANT, and JENNIFER KEISER. "Comparison of novel and existing tools for studying drug sensitivity against the hookworm Ancylostoma ceylanicum in vitro." Parasitology 139, no. 3 (2012): 348–57. http://dx.doi.org/10.1017/s0031182011001934.

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SUMMARYThe motility assay is the current gold standard for evaluating drug effects on hookworm larvae and adults, however, among other drawbacks the assay is time consuming, and prone to individual subjectivity. We evaluated six alternative in vitro assays, namely the feeding inhibition assay, the colourimetric AlamarBlue®, MTT formazan and acid phosphatase activity assays, as well as isothermal calorimetry and the xCELLigence System using Ancylostoma ceylanicum third-stage larvae, stimulated third-stage larvae and adults. The performances of the assays were compared to the motility assay usin

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Figueiró, Luciana Rizzieri, Luana Christine Comerlato, Marcia Vignoli Da Silva, José Ângelo Silveira Zuanazzi, Gilsane Lino Von Poser, and Ana Luiza Ziulkoski. "Original Article. Toxicity of Glandularia selloi (Spreng.) Tronc. leave extract by MTT and neutral red assays: influence of the test medium procedure." Interdisciplinary Toxicology 9, no. 1 (2016): 25–29. http://dx.doi.org/10.1515/intox-2016-0004.

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Abstract Cytotoxicity assays using cell cultures may be an alternative to assess biological toxicity of plant extracts with potential phytotherapeutic properties. This study compared three methods to prepare culture media for the exposure of Vero cells to plant extracts. Leaves of Glandularia selloi (Spreng.) Tronc. were used to prepare culture medium with aqueous extract, extract in culture medium and methanol extract. Toxicity was assessed using the MTT and neutral red (NR) assays. In general, alterations in the cellular functions were found in all extracts and assays. Cytotoxic effect occur

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Choghazardi, Yazdan, Hosein Azimian, Alireza Montazer Abadi, Milad Mohammadi Khoshisani, Fereshteh Vaziri Nezamdoust, and Hamid Gholamhosseinian. "Radiosensitivity Enhancement using Triptorelin Conjugated Bismuth Sulfide Nanoparticles (Bi2S3@BSA) in Radiotherapy for Breast Cancer Cells." Journal of Nanomaterials 2023 (May 6, 2023): 1–11. http://dx.doi.org/10.1155/2023/5485632.

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The aim of this study was to assess the radiosensitivity of bismuth sulfide nanoparticles conjugated with a synthetic agonist analog of gonadotropin-releasing hormones in targeted radiotherapy for breast cancer. After synthesis and characterization of nanoparticles, cytotoxicity of nanoparticles was measured by MTT assay, and the survival fraction was determined by colony formation assay. Finally, flow cytometry was performed to identify the mechanism of radiosensitization. Characterization results determined the spherical shape of Bi2S3@BSA with an average size of 8.649 ± 1.6 nm, and Fourier

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Pratama, Fajri Rahendra, Annisa Permata Sari, Aprilia Susanti, Hasya Syakira, Amilia Salsabila, and Dessy Arisanty. "Potensi Eugenol Fraksi Etil Asetat Bunga Cengkeh terhadap Proliferasi Kanker Serviks (HeLa Cell Line)." Jurnal Kesehatan Andalas 13, no. 1 (2024): 14. http://dx.doi.org/10.25077/jka.v13i1.2326.

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<p>Cervix cancer, also known as cervical cancer, is a type of cancer that is caused mainly by oncogenic Human Papillomavirus (HPV), which attacks the cervix. One of the plants that can be used in medicine is clove. <strong>Objective</strong>: To analyzed the effect of a fraction of ethyl acetate eugenol of clove flower for the proliferation and migration of HeLa cells. <strong>Methods</strong>: This was an in vitro experiment. Ethyl acetate fraction was tested in vitro with the MTT Assay method. A scratch Assay is done to determine the migratory power of cancer ce

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Susanty, Adriani, Mira Febrina, Dian Sanita Putri, Ihsan Ikhtiarudin, Fatma Sri Wahyuni, and Dachriyanus Dachriyanus. "Cytotoxic Activity of Ethyl Acetate Extract from Voacanga foetida (Bl.) Rolfe Leaves Against T47D Breast Cancer Cells." Borneo Journal of Pharmacy 6, no. 1 (2023): 8–14. http://dx.doi.org/10.33084/bjop.v6i1.3499.

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The cytotoxicity of ethyl acetate extract from Tampa badak (Voacanga foetida (Bl.) Rolfe) leaves against Artemia salina leach larvae was determined using the brine shrimp lethality test (BSLT) method and was evaluated against T47D breast cancer cells using MTT assay method. The result of BSLT showed a consistent result with MTT assay, which is the result obtained that ethyl acetate extract is very toxic against A. salina Leach larvae with LC50 value of 8.61 µg/mL and very cytotoxic against T47D breast cancer cells with IC50 values of 0.87; 0.66; and 0.95 µg/mL at the 24, 48 and 72 hours of inc

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Trintinaglia, L., E. Bianchi, LB Silva, CA Nascimento, FR Spilki, and AL Ziulkoski. "Cytotoxicity assays as tools to assess water quality in the Sinos River basin." Brazilian Journal of Biology 75, no. 2 suppl (2015): 75–80. http://dx.doi.org/10.1590/1519-6984.0113.

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<p>Cytotoxicity assays using cell cultures may be an alternative to assess biological toxicity of surface waters and may help to improve the control of water quality. This study compared two methods to prepare culture media for the exposure of Hep-2 cells to water samples collected from the Rolante River, an important affluent of the Sinos River. The toxicity was evaluated using the MTT and neutral red assays. Two methods were used to prepare culture media. In method 1, the sample was diluted at 1:1, 1:10, 1:100, 1:1000, 1:10.000 (v/v, sample/medium) in a standard culture medium; in meth

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Ma, Lingyan, and Yufei Zou. "TF-1 Cell Proliferation Assay Method for Estimating Bioactivity of Nerve Growth Factor." Journal of Applied Virology 2, no. 2 (2013): 32. http://dx.doi.org/10.21092/jav.v2i2.5.

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<p>Nerve growth factor (NGF) is a neurotrophic factor responsible for the differentiation and survival of sympathetic and sensory neurons as well as selective populations of cholinergic neurons. The MTT assay is to add the chromogenic substance MTT after the NGF has stimulated the TF1 cells, to conduct a colorimetric analysis on the micro plate reader, then to compare the results with a standard curve to arrive at the biological activity of the test substance. To use the MTT assay to measure the biological activity of the NGF, it is easy to handle, with good and reliable results, repeata

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Emter, Roger, and Andreas Natsch. "A fast Resazurin-based live viability assay is equivalent to the MTT-test in the KeratinoSens assay." Toxicology in Vitro 29, no. 4 (2015): 688–93. http://dx.doi.org/10.1016/j.tiv.2015.02.003.

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Borah, Rajiv, Ashok Kumar, Monoj Kumar Das, and Anand Ramteke. "Surface functionalization-induced enhancement in surface properties and biocompatibility of polyaniline nanofibers." RSC Advances 5, no. 60 (2015): 48971–82. http://dx.doi.org/10.1039/c5ra01809a.

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Surface functionalization by glutaraldehyde effectively enhanced hydrophilicity of polyaniline nanofibers (PNFs) rendering them with improved biocompatibility as revealed by MTT assay and membrane stability test.

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Milek, Michal, Dana Marcinčáková, Tomáš Csank, et al. "Real-time monitoring of cadmium toxicity in rabbit kidney cells." Acta Veterinaria Brno 84, no. 4 (2015): 351–56. http://dx.doi.org/10.2754/avb201584040351.

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The aim of this study was to investigate the toxic effect of the metal salt cadmium chloride dihydrate on the rabbit kidney cell line using the xCELLigence system or real-time cell analyser (RTCA), and to compare this relatively new method with standard biological cytotoxicity assays. This system provides real-time monitoring of cell behaviour and proliferative activity during the whole time of experiment. Moreover, after 24 h exposure of cells to cadmium, colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-difenyl tetrazolium bromide (MTT) test was used to measure the metabolic activity and cytotox

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Müller, Anna Sonja, Klara Janjić, Gunpreet Oberoi, et al. "Deferoxamine but Not Dimethyloxalylglycine, L-Mimosine, or Cobalt Dichloride Can Interfere with the MTT Assay." BioMed Research International 2018 (November 18, 2018): 1–6. http://dx.doi.org/10.1155/2018/5872865.

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Hypoxia mimetic agents (HMAs) have been shown to have a positive influence on cellular functions in a multitude of tissue regenerative strategies. Novel experimental approaches use biomaterials as carriers for controlled delivery of these HMAs. Here, the cytotoxic aspects of biocompatibility are of key relevance. The MTT assay is widely used to evaluate cytotoxicity and proliferation. Based on the implications from the proceeding research we hypothesized that specific HMAs such as deferoxamine at high concentrations can interfere with the MTT assay. Thus, the aim of this study was to test the

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Kalpana C S, Kalaivani P, and Vanitha V. "Phytochemical Screening and Effect of Cymodocea serrulata on HepG2-Human Hepatocellular Carcinoma Cell Line." International Journal of Research in Pharmaceutical Sciences 11, no. 2 (2020): 1684–91. http://dx.doi.org/10.26452/ijrps.v11i2.2058.

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Cymodocea serrulata, a seagrass commonly known as karumbupassi has been used as a food and also as a medicine by coastal region people and by fishermen while traveling in the sea. It is used as a tranquilizer for babies as it has a soothing quality, it helps during pregnancy and against cough and malaria. C.serrulata is seen abundant in South Indian coastal region. Although there is a report on the antibacterial, antioxidant, and anti-inflammatory property of C.serrulata, there are no evident details on phyto-compounds present in it. Invitro antiproliferative test was performed by MTT (methylt

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Pieters, R., AH Loonen, DR Huismans, et al. "In vitro drug sensitivity of cells from children with leukemia using the MTT assay with improved culture conditions." Blood 76, no. 11 (1990): 2327–36. http://dx.doi.org/10.1182/blood.v76.11.2327.2327.

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Abstract The knowledge about drug resistance in childhood leukemias and acute lymphoblastic leukemia (ALL) in general is limited. This is because of the lack of a suitable in vitro drug sensitivity assay, which is in part due to low in vitro ALL cell survival. We recently adapted the highly efficient 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay to test cells from ALL patients and showed that its results were comparable with those of the DiSC assay, up to now the most valid but laborious assay. In this study, in vitro drug sensitivity was assessed in cells from 82 c

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Pieters, R., AH Loonen, DR Huismans, et al. "In vitro drug sensitivity of cells from children with leukemia using the MTT assay with improved culture conditions." Blood 76, no. 11 (1990): 2327–36. http://dx.doi.org/10.1182/blood.v76.11.2327.bloodjournal76112327.

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The knowledge about drug resistance in childhood leukemias and acute lymphoblastic leukemia (ALL) in general is limited. This is because of the lack of a suitable in vitro drug sensitivity assay, which is in part due to low in vitro ALL cell survival. We recently adapted the highly efficient 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay to test cells from ALL patients and showed that its results were comparable with those of the DiSC assay, up to now the most valid but laborious assay. In this study, in vitro drug sensitivity was assessed in cells from 82 children w

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Jordan, Joseph P., Christopher M. Hand, Luis Duarte, and Perry Black. "Test for Chemotherapeutic Sensitivity of Cerebral Gliomas: Use of the Colorimetric MTT Assay." Rehabilitation Oncology 11, no. 1 (1993): 22. http://dx.doi.org/10.1097/01893697-199311010-00016.

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Thiagarajan, Magesh Karuppur, Deepak Chandrasekharan, Ramya Mahalingam, and Aravindhan Ravi. "Evaluation of in vitro anti-cancer potential and apoptotic profile of ethanolic plant extract of Wrightia tinctoria against oral cancer cell line." Journal of Oral and Maxillofacial Pathology 28, no. 2 (2024): 211–15. http://dx.doi.org/10.4103/jomfp.jomfp_32_24.

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Abstract Background: For decades, Wrightia tinctoria (W. tinctoria) has been important in the field of traditional medicine. The identification and isolation of numerous chemically potent and pharmaceutically advantageous phytochemicals isolated from this plant extract has contributed to its resurrection into evidence-based modern medicine. This plant is inexpensive and indigenous to the Indian subcontinent. Materials and Method: An in vitro study was carried out using KB (KERATIN-forming tumour cell line HeLa) oral cancer cell line. The control drug doxorubicin along with the test drug W. tin

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Pawlak, Aleksandra, Bożena Obmińska-Mrukowicz, Iwona Zbyryt, and Andrzej Rapak. "In vitro drug sensitivity in canine lymphoma." Journal of Veterinary Research 60, no. 1 (2016): 55–61. http://dx.doi.org/10.1515/jvetres-2016-0009.

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AbstractIntroduction: Due to the high heterogeneity of canine lymphoma, the aim of the present study was to test in vitro the chemosensitivity of canine high-grade primary lymphoma cells to various cytostatic drugs commonly used to treat dogs: 4-HO-cyclophosphamide, doxorubicin, dexamethasone, prednisolone, vincristine, etoposide, chlorambucil, lomustine, and cytosine arabinoside. Material and Methods: To determine the cell viability and drug ability to induce apoptosis two different tests were used: an MTT assay and annexin V/propidium iodide staining. Results: Both in vitro tests were found

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Huang, Zengfa, Cheng Cheng, Jing Wang, et al. "Surface Properties and MC3T3-E1 Cell Response of Cortical Bone Allografts Modified with Low-Concentration Phosphoric Acid." Cellular Physiology and Biochemistry 41, no. 4 (2017): 1572–83. http://dx.doi.org/10.1159/000470823.

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Background/Aims: This experimental study aimed to evaluate the effect of low-concentration phosphoric acid on the surface structure of cortical allografts. Methods: Allogenic cortical bones were obtained from femurs and tibias of New Zealand white rabbits. The bones were modified by treatment with various concentrations of phosphoric acid (10%, 20% or 30%) for 10, 30 or 60 minutes, then evaluated by the following methods: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and LIVE/DEAD assay, alkaline phosphatase (ALP) assay, biomechanical properties testing, contact angle

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Robiansyah, Robiansyah, Debby Saputera, and Rahmad Arifin. "TOXICITY TEST OF SMALL WHITE GINGER EXTRACT ON BHK-21 FIBROBLAST CELLS IN VITRO." Dentino : Jurnal Kedokteran Gigi 5, no. 1 (2020): 6. http://dx.doi.org/10.20527/dentino.v5i1.8113.

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Background: Denture stomatitis is inflammation of the oral mucosa which supporting the denture that caused by Candida albicans. Candida albicans contamination can be prevented by immersing dentures into denture cleanser solution. One of the alternative ingredients that can be used as denture cleanser is small white ginger rhizome (Zingiber officinale var. Amarum). Objective: This study aimed to analyze whether small white ginger extract (Zingiber officinale var. Amarum) was toxic to BHK-21 fibroblast cells using the MTT assay method. Method: This study was conducted in 7 groups. Five groups co

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Gayathri, V., S. Ananthi, and Hannah R. Vasanthi. "Antihyperlipidemic Potential of Polyphenol and Glycoside RichNerium oleanderFlower against Triton WR-1339-Induced Hyperlipidemia in Experimental Sprague Dawley Rats." Journal of Chemistry 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/825290.

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Nerium oleanderLinn. (NO), an evergreen cardiac glycoside-rich shrub is used as folklore medicine in China to treat many diseases. It exhibits a wide spectrum of bioactivities but there were not much scientific reports on the bioactivity ofN. oleanderflowers. In the present study, we have evaluated the toxicity profile of the 50% hydroethanolic extracts ofNerium oleanderflowers (ENO) usingin vitrobrine shrimp lethality assay and MTT cytotoxicity assay andin vivoacute toxicity test as per the OECD guidelines. The antihyperlipidemic activity of the ENO was also studied using Triton WR-1339-induc

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Adam, Mardiana A., and Asti Meizarini. "Sitotoksisitas pemutih gigi berdasarkan konsentrasi bahan." Journal of Dentomaxillofacial Science 9, no. 2 (2010): 116. http://dx.doi.org/10.15562/jdmfs.v9i2.241.

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The aim of this study was to evaluate the cytotoxicity of several tooth whitening material,carbamide peroxide 10%, 15%, 20% and hydrogen peroxide 38% toward BHK-21 cellusing MTT assay. Each well of microplates which used for the test were aliquotted BHK21cell suspension, after that the test solution were added to eight well each group,respectively. The result showed that percentages of the living cell at 10% carbamideperoxide group = 86,73%; 15% = 81,22%; 20% = 81,82%; 38% hidrogen peroksida =64,08%, respectively. Anova test and LSD showed no significant difference between 10%,15%, 20% groups,

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Ngurah, Budiana I. Gusti M., Jumina Jumina, Chairil Anwar, Sunardi Sunardi, and Mustofa Mustofa. "Synthesis and In Vitro Evaluation of C-methylcalix[4]resorcinaryl octacinnamate and C-methylcalix[4]resorcinaryl octabenzoate as the Sunscreen." Indonesian Journal of Chemistry 17, no. 1 (2017): 63. http://dx.doi.org/10.22146/ijc.23575.

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The present study was aimed to synthesize and evaluate sunscreen activity of C-methylcalix[4]resorcinaryl octacinnamate and C-methylcalix[4]resorcinaryl octabenzoate. The target compounds were synthesized in 2 steps. They were a synthesis of C-methylcalix[4]-resorcinarene via acid catalyzed the condensation of resorcinol and acetaldehyde by using HCl catalyst, followed by esterification using cinnamoyl chloride and benzoyl chloride. The characterization of the target compounds was performed by IR, 1H-NMR, 13C-NMR, and LC-MS spectrometers. The sunscreen activity test was conducted by spectrosco

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Lee, Donghee, Jun-Beom Park, Dani Song, Hye-Min Kim, and Sin-Young Kim. "Cytotoxicity and Mineralization Potential of Four Calcium Silicate-Based Cements on Human Gingiva-Derived Stem Cells." Coatings 10, no. 3 (2020): 279. http://dx.doi.org/10.3390/coatings10030279.

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The aim of this study was to evaluate the cytotoxicity and mineralization potential of four calcium silicate-based cements on human gingiva-derived stem cells (GDSCs). The materials evaluated in the present study were ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), Endocem Zr (Maruchi), and RetroMTA (BioMTA). Experimental disks of 6 mm in diameter and 3 mm in height were produced and placed in a 100% humidified atmosphere for 48 h to set. We evaluated the cytotoxic effects of the cements using methyl-thiazoldiphenyl-tetrazolium (MTT) and live/dead staining assays. We u

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Lima Feitosa, Mariana, Deiziane Viana da Silva Costa, Izabell Maria Martins Teixeira, et al. "Benzydamine induces apoptosis in astrocytes in vitro." Journal of Health & Biological Sciences 12, no. 1 (2024): 1–7. http://dx.doi.org/10.12662/2317-3076jhbs.v12i1.5271.p1-7.2024.

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Objective: the present work aims to evaluate the Benzydamine (BZD) effect on cell viability in astrocyte culture and investigated the death mechanism involved with its cytotoxic effect. Methods: in order to evaluate cell viability, the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay was used, while flow cytometry was used to verify cell damage. The immunofluorescence assay was used to verify the expression of the marker’s caspase-8, caspase9 and p65 subunit of Factor nuclear kappa B (NFκB). A statistical analysis for MTT assay and Flow Cytometry were made usi

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Jiao, Guozheng, Xiong He, Xin Li, et al. "Limitations of MTT and CCK-8 assay for evaluation of graphene cytotoxicity." RSC Advances 5, no. 66 (2015): 53240–44. http://dx.doi.org/10.1039/c5ra08958a.

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Maru, Viral, Omkar Shetty, Uma Dixit, Vishal G. Warke, Mansi Khande, and Shraddha Mane. "SHED′s Response to Various Pulpotomy Materials: Cytotoxicity and Gene Expression Analysis." Journal of Clinical Pediatric Dentistry 45, no. 6 (2021): 406–13. http://dx.doi.org/10.17796/1053-4625-45.6.7.

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Purpose : To examine the cytotoxicity and genetic expression of SHEDs cultured in eluates of various calcium silicate based pulpotomy materials. Study design : MTT assay, flow cytometry, alizarin red staining and scratch assay was used to assess the cellular viability, apoptosis, calcium matrix deposits and cell migration respectively. The gene expression of ALP, OCN and BMP -2, were measured with rtPCR. One way ANNOVA and Bonferroni post test was used for statistical analysis. Results : MTT assay analysis reported that all the test specimen had no cytotoxic effects. The highest number of live

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Lima, Karina Motta Melo, Luana França Calandrini de Azevedo, Jorge Dores Rissino та ін. "Anticancer Potential and Safety Profile of β-Lapachone In Vitro". Molecules 29, № 6 (2024): 1395. http://dx.doi.org/10.3390/molecules29061395.

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Ipê is a plant of the Bignoniaceae family. Among the compounds extracted from this tree, lapachol is notable because its structural modification allows the production of β-lapachone, which has anticancer properties. The objective of this work was to test this hypothesis at a cellular level in vitro and assess its potential safety for use. The following tests were performed: MTT cell viability assay, apoptotic index determination, comet assay, and micronucleus test. The results showed that β-lapachone had a high cytotoxic capacity for all cell lines tested: ACP02 (gastric adenocarcinoma cells),

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Wakode, S., T. Trushna, N. Wakode, A. Thakare, R. Bharshankar, and S. Manjunatha. "Use of MTT Assay for Proliferation of U937 Cell Line and its Inter-rater Reliability - Best Taken with a Grain of Salt." Kathmandu University Medical Journal 18, no. 4 (2020): 396–401. http://dx.doi.org/10.3126/kumj.v18i4.49253.

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Background Biomedical research frequently employs immortal human cell lines to study various physiological and pathological topics of interest. An important problem that is often ignored is the question of reliability of observations made while using a particular assay on cell lines, especially MTT Assay. Recent studies have questioned its reliability by highlighting the diverse intracellular environmental factors that affect accuracy of MTT assay results. Objective To assess the inter-rater reliability of MTT assay while observing the differential effect of addition of fetal bovine seru

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Jo, Hwa Yeon, Yona Kim, Hyung Woo Park, et al. "The Unreliability of MTT Assay in the Cytotoxic Test of Primary Cultured Glioblastoma Cells." Experimental Neurobiology 24, no. 3 (2015): 235–45. http://dx.doi.org/10.5607/en.2015.24.3.235.

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Maehara, Yoshihiko, Tetsuya Kusumoto, Hiroki Kusumoto, Hideaki Anai, and Keizo Sugimachi. "Sodium Succinate Enhances the Colorimetric Reaction of the in vitro Chemosensitivity Test: MTT Assay." Oncology 45, no. 6 (1988): 434–36. http://dx.doi.org/10.1159/000226660.

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Đurđević, Sanja, Katarina Šavikin, Jelena Živković, et al. "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L." J Supercritical Fluids 133, Part I (2018): 225–32. https://doi.org/10.1016/j.supflu.2017.10.021.

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Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, thepomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-criticalfluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile,carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids(PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), o

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Sagala, Evayanti Meiliana, and Jansen Silalahi. "Wound Healing Activities of Hydrolyzed Virgin Coconut Oil (HVCO) and Fucoidan Combination: An In Vitro Assay." Asian Journal of Pharmaceutical Research and Development 7, no. 3 (2019): 40–45. http://dx.doi.org/10.22270/ajprd.v7i3.532.

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combination, in the NIH 3T3 cell line using in vitro assay, and compared with single HVCO and single fucoidan. Methods: NIH 3T3 Cell viability and proliferation were assessed using the MTT method, migration activity was assessed using scratch wound healing assays and expression of COX-2 and VEGF protein were determined using immunocytochemistry (ICC). Results: The results from the proliferative activity assay show that the effective concentrations for all samples were 31.25 μg /ml. NIH 3T3 cells migration activity assay showed that the best combination of the HVCO and fucoidan was 50:50. From

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Jabbar, Hajria, Prihartini Widiyanti, Adanti Wido Paramadini, Dina Kartika Putri, and Andini Isfandiary. "In Vitro Characterization of Poly-Glycolyc Lactic-Co Acid (PLGA) –Collagen Based on Red Snapper Fish Scales (Lutjanus Sp.) Coating Chitosan as Duramater Artificial Candidate." Folia Medica Indonesiana 56, no. 3 (2020): 229. http://dx.doi.org/10.20473/fmi.v56i3.22191.

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Head trauma is the third cause of deaths with a high rank, causing severe head injury for 25.5%-54.9%. This study has been conducted by making a replacement layer of the brain (dura) to overcome the dural defect's impact by utilizing waste fish scales red snapper (Lutjanus Sp.). Synthesis brain membranes lining processed by casting method with each various chitosan coating concentrations of 1%, 1.5%, and 2%, then dried using vacuum dry. The samples were then characterized by tensile tests, FTIR, SEM, and MTT Assay. FTIR test results showed that red snipper scales could produce collagen powder

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Correa, Regianne Maciel dos Santos, Tatiane Cristina Mota, Adriana Costa Guimarães, Laís Teixeira Bonfim, Rommel Rodriguez Burbano, and Marcelo de Oliveira Bahia. "Cytotoxic and Genotoxic Effects of Fluconazole on African Green Monkey Kidney (Vero) Cell Line." BioMed Research International 2018 (November 1, 2018): 1–7. http://dx.doi.org/10.1155/2018/6271547.

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Fluconazole is a broad-spectrum triazole antifungal that is well-established as the first-line treatment for Candida albicans infections. Despite its extensive use, reports on its genotoxic/mutagenic effects are controversial; therefore, further studies are needed to better clarify such effects. African green monkey kidney (Vero) cells were exposed in vitro to different concentrations of fluconazole and were then evaluated for different parameters, such as cytotoxicity (MTT/cell death by fluorescent dyes), genotoxicity/mutagenicity (comet assay/micronucleus test), and induction of oxidative st

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Musfiroh, Ida, Angga Geganaputra, Ajeng Diantini, Yasmiwar Susilawati, and Muchtaridi Muchtaridi. "Antiproliferation Assay of Essential Oil of Curcuma Rhizoma (Curcuma xanthorrhiza Roxb.) Against P388 Leukemia Cell." Indonesian Journal of Pharmaceutical Science and Technology 7, no. 3 (2020): 100. http://dx.doi.org/10.24198/ijpst.v7i3.27210.

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Leukemia or blood cancer is a disease which marked by abnormal increasing of blood producer`s cells. Chemotherapies which used as anticancer have a many adverse effect and toxicity. The volatile oil of turmeric rhizome (Curcuma xanthorriza) contains sesquiterpene which has an pharmacological activity. The aimed of this research to assay the antiproliferation activity of volatile oil from curcuma rhizome to leukemia P388 cells using MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl tetrazolium bromide) method. The methods were contain of identification of volatile oil (produced from distillation

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Hafiz, Muhammad Arsalan Altaf Hussain Saira Naseem Zeemal Seemab Amin Farooq Saleem Sania Iftikhar Anum Shahzadi Hina Mumtaz. "PHYTOCHEMICAL CHARACTERIZATION AND ASSESSMENT OF ANTI-CANCER ACTIVITY OF SELECTED MEDICINAL PLANTS AGAINST VERO CELL LINES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 07 (2019): 13584–93. https://doi.org/10.5281/zenodo.3266243.

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<em>The objective of this study was to discover the scientific source these plants have much inhibitory effects against Vero cell lines. Medicinal plants have secondary metabolites in which more quality against many type of disordered and like flavonoids, phenol, taninns, and some other that have very good and special properties are occur them. DPPH assay screen test on the TLC shows very much anticancer activity. MTT assay proved that these plants are abundant inhibitory activity against VERO cell liens cell. These shows we can reduce that death rate of cancer and also control that cancer tha

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Amalia, Leni, Aini Nasution, Syafruddin Ilyas, Martina Amalia, and Indra Nasution. "Viability Test of Osteoblast Cells After Application of Hydroxyapatite Nanoparticle from Unam Snail’s Shells In-vitro Examination." International Journal of Clinical Oral and Maxillofacial Surgery 10, no. 1 (2024): 1–7. https://doi.org/10.11648/j.ijcoms.20241001.11.

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The bone defect reconstruction process can use hydroxyapatite is osteoconductive and can retain the original biocompatible shape to enhance hydroxyapatite with osteogenic proteins. To analyze the most appropriate concentration of hydroxyapatite nanoparticles using the MTT Assay method and to test the viability of osteoblast cells after being given hydroxyapatite nanoparticle (nHA) derived from unam snail shells. The fabrication of hydroxyapatite nanoparticles from unam snail shells using a mechanical-chemical combination method. Osteoblast cells are obtained from Calvaria rats after being cult

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Trivedi, Mahendra Kumar, Dahryn Trivedi, Alice Branton, and Snehasis Jana. "Study of VDR Expression after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation Using Cellular Models." International Journal of Biochemistry & Physiology 6, no. 1 (2021): 1–9. https://doi.org/10.5281/zenodo.10279559.

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The present study was designed to evaluate the VDR (vitamin D receptor) expression after treatment with the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on various cell lines in DMEM medium using values of relative quantification (RQ) by RTPCR among different groups. The test formulation constituents were divided into two parts; one part defined

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