Relevant bibliographies by topics / Multilocus sequence typing (MLST)

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Academic literature on the topic 'Multilocus sequence typing (MLST)'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 January 2023

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Journal articles on the topic "Multilocus sequence typing (MLST)"

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Brown, Rebecca J., Matthew T. G. Holden, O. Brad Spiller, and Victoria J. Chalker. "Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae." Journal of Clinical Microbiology 53, no. 10 (2015): 3195–203. http://dx.doi.org/10.1128/jcm.01301-15.

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Mycoplasma pneumoniaeis a major human respiratory pathogen causing both upper and lower respiratory disease in humans of all ages, and it can also result in other serious extrapulmonary sequelae. A multilocus sequence typing (MLST) scheme forM. pneumoniaewas developed based on the sequences of eight housekeeping genes (ppa,pgm,gyrB,gmk,glyA,atpA,arcC, andadk) and applied to 55M. pneumoniaeclinical isolates and the two type strains M129 and FH. A total of 12 sequence types (STs) resulted for 57M. pneumoniaeisolates tested, with a discriminatory index of 0.21 STs per isolate. The MLST loci used in this scheme were shown to be stable in 10 strains following 10 sequential subculture passages. Phylogenetic analysis of concatenated sequences of the eight loci indicated two distinct genetic clusters that were directly linked to multilocus variable-number tandem repeat analysis (MLVA) type. Genetic MLST clustering was confirmed by genomic sequence analysis, indicating that the MLST scheme developed in this study is representative of the genome. Furthermore, this MLST scheme was shown to be more discriminatory than both MLVA and P1 typing for theM. pneumoniaeisolates examined, providing a method for further and more detailed analysis of observed epidemic peaks ofM. pneumoniaeinfection. This scheme is supported by a public Web-based database (http://pubmlst.org/mpneumoniae).
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Adiri, Roni S., Uri Gophna, and Eliora Z. Ron. "Multilocus sequence typing (MLST) ofEscherichia coliO78 strains." FEMS Microbiology Letters 222, no. 2 (2003): 199–203. http://dx.doi.org/10.1016/s0378-1097(03)00295-7.

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Pasic, Lana, Lidia Goterris, Mercedes Guerrero-Murillo, et al. "Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii." Journal of Fungi 6, no. 4 (2020): 259. http://dx.doi.org/10.3390/jof6040259.

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Pneumocystis jirovecii is an opportunistic human pathogenic fungus causing severe pneumonia mainly in immunocompromised hosts. Multilocus sequence typing (MLST) remains the gold standard for genotyping of this unculturable fungus. However, the lack of a consensus scheme impedes a global comparison, large scale population studies and the development of a global MLST database. To overcome this problem this study compared all genetic regions (19 loci) currently used in 31 different published Pneumocystis MLST schemes. The most diverse/commonly used eight loci, β-TUB, CYB, DHPS, ITS1, ITS1/2, mt26S and SOD, were further assess for their ability to be successfully amplified and sequenced, and for their discriminatory power. The most successful loci were tested to identify genetically related and unrelated cases. A new consensus MLST scheme consisting of four genetically independent loci: β-TUB, CYB, mt26S and SOD, is herein proposed for standardised P. jirovecii typing, successfully amplifying low and high fungal burden specimens, showing adequate discriminatory power, and correctly identifying suspected related and unrelated isolates. The new consensus MLST scheme, if accepted, will for the first time provide a powerful tool to investigate outbreak settings and undertake global epidemiological studies shedding light on the spread of this important human fungal pathogen.
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Baldo, Laura, Julie C. Dunning Hotopp, Keith A. Jolley, et al. "Multilocus Sequence Typing System for the Endosymbiont Wolbachia pipientis." Applied and Environmental Microbiology 72, no. 11 (2006): 7098–110. http://dx.doi.org/10.1128/aem.00731-06.

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ABSTRACT The eubacterial genus Wolbachia comprises one of the most abundant groups of obligate intracellular bacteria, and it has a host range that spans the phyla Arthropoda and Nematoda. Here we developed a multilocus sequence typing (MLST) scheme as a universal genotyping tool for Wolbachia. Internal fragments of five ubiquitous genes (gatB, coxA, hcpA, fbpA, and ftsZ) were chosen, and primers that amplified across the major Wolbachia supergroups found in arthropods, as well as other divergent lineages, were designed. A supplemental typing system using the hypervariable regions of the Wolbachia surface protein (WSP) was also developed. Thirty-seven strains belonging to supergroups A, B, D, and F obtained from singly infected hosts were characterized by using MLST and WSP. The number of alleles per MLST locus ranged from 25 to 31, and the average levels of genetic diversity among alleles were 6.5% to 9.2%. A total of 35 unique allelic profiles were found. The results confirmed that there is a high level of recombination in chromosomal genes. MLST was shown to be effective for detecting diversity among strains within a single host species, as well as for identifying closely related strains found in different arthropod hosts. Identical or similar allelic profiles were obtained for strains harbored by different insect species and causing distinct reproductive phenotypes. Strains with similar WSP sequences can have very different MLST allelic profiles and vice versa, indicating the importance of the MLST approach for strain identification. The MLST system provides a universal and unambiguous tool for strain typing, population genetics, and molecular evolutionary studies. The central database for storing and organizing Wolbachia bacterial and host information can be accessed at http://pubmlst.org/wolbachia/ .
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Shibata, Yusuke, Le Hong Thuy Tien, Ryohei Nomoto, and Ro Osawa. "Development of a multilocus sequence typing scheme for Streptococcus gallolyticus." Microbiology 160, no. 1 (2014): 113–22. http://dx.doi.org/10.1099/mic.0.071605-0.

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Streptococcus gallolyticus is often found as a member of the normal gut microflora in various animals. However, it has been reported to cause mastitis in cattle, septicaemia in pigeons, and meningitis, septicaemia and endocarditis in humans. However, little is known about the epidemiology and crucial virulence factors of S. gallolyticus. To help address these issues, we developed a multilocus sequence typing (MLST) scheme for S. gallolyticus. Seven housekeeping gene fragments were sequenced from each of 58 S. gallolyticus isolates collected from diverse origins and sources. The MLST scheme had good discriminatory ability. The 63 strains, including the 5 whole genome sequenced strains examined, resolved into 57 sequence types (STs), with 52 STs represented by only a single strain. With respect to the identification of S. gallolyticus subspecies (i.e. S. gallolyticus subsp. gallolyticus, S. gallolyticus subsp. pasteurianus and S. gallolyticus subsp. macedonicus), the results of biochemical tests and DNA–DNA hybridization were in high concordance with those of the MLST scheme. The MLST scheme developed in this study may be a useful tool capable of replacing the conventional methods used for S. gallolyticus subspecies identification. The results of this study suggest that the biology and virulence of two pathogenic S. gallolyticus subspecies (i.e. S. gallolyticus subsp. gallolyticus and S. gallolyticus subsp. pasteurianus) are very different. The MLST scheme offers researchers a valuable typing tool that will promote further investigation of the epidemiology of S. gallolyticus.
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Fernández-Naval, Candela, Maider Arando, Mateu Espasa, et al. "Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona." Future Microbiology 16, no. 13 (2021): 967–76. http://dx.doi.org/10.2217/fmb-2021-0037.

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Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.
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de Been, Mark, Mette Pinholt, Janetta Top, et al. "Core Genome Multilocus Sequence Typing Scheme for High-Resolution Typing of Enterococcus faecium." Journal of Clinical Microbiology 53, no. 12 (2015): 3788–97. http://dx.doi.org/10.1128/jcm.01946-15.

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Enterococcus faecium, a common inhabitant of the human gut, has emerged in the last 2 decades as an important multidrug-resistant nosocomial pathogen. Since the start of the 21st century, multilocus sequence typing (MLST) has been used to study the molecular epidemiology ofE. faecium. However, due to the use of a small number of genes, the resolution of MLST is limited. Whole-genome sequencing (WGS) now allows for high-resolution tracing of outbreaks, but current WGS-based approaches lack standardization, rendering them less suitable for interlaboratory prospective surveillance. To overcome this limitation, we developed a core genome MLST (cgMLST) scheme forE. faecium. cgMLST transfers genome-wide single nucleotide polymorphism (SNP) diversity into a standardized and portable allele numbering system that is far less computationally intensive than SNP-based analysis of WGS data. TheE. faeciumcgMLST scheme was built using 40 genome sequences that represented the diversity of the species. The scheme consists of 1,423 cgMLST target genes. To test the performance of the scheme, we performed WGS analysis of 103 outbreak isolates from five different hospitals in the Netherlands, Denmark, and Germany. The cgMLST scheme performed well in distinguishing between epidemiologically related and unrelated isolates, even between those that had the same sequence type (ST), which denotes the higher discriminatory power of this cgMLST scheme over that of conventional MLST. We also show that in terms of resolution, the performance of theE. faeciumcgMLST scheme is equivalent to that of an SNP-based approach. In conclusion, the cgMLST scheme developed in this study facilitates rapid, standardized, and high-resolution tracing ofE. faeciumoutbreaks.
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SIDORENKO, S. V., V. S. SOLOMKA, O. S. KOZhUShNAYa, et al. "Methods for typing std pathogens (N. Gonorrhoeae, C. Trachomatis, T. Pallidum)." Vestnik dermatologii i venerologii 86, no. 3 (2010): 12–21. http://dx.doi.org/10.25208/vdv781.

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Phenotypic methods were initially used for bacterial typing yet they have a number of drawbacks limiting their use. Methods of molecular and genetic typing have become wide-spread today. Among these methods, bacterial typing based on multilocus sequence typing (Multilocus Sequence Typing - MLST) has been developing at the fastest rate. However, schemes of molecular and genetic typing of STD pathogens as compared to other bacteria are insufficiently developed, which considerably complicates the planning of measures aimed at the reduction of their spread.
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Tanigawa, Kana, and Koichi Watanabe. "Multilocus sequence typing reveals a novel subspeciation of Lactobacillus delbrueckii." Microbiology 157, no. 3 (2011): 727–38. http://dx.doi.org/10.1099/mic.0.043240-0.

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Currently, the species Lactobacillus delbrueckii is divided into four subspecies, L. delbrueckii subsp. delbrueckii, L. delbrueckii subsp. bulgaricus, L. delbrueckii subsp. indicus and L. delbrueckii subsp. lactis. These classifications were based mainly on phenotypic identification methods and few studies have used genotypic identification methods. As a result, these subspecies have not yet been reliably delineated. In this study, the four subspecies of L. delbrueckii were discriminated by phenotype and by genotypic identification [amplified-fragment length polymorphism (AFLP) and multilocus sequence typing (MLST)] methods. The MLST method developed here was based on the analysis of seven housekeeping genes (fusA, gyrB, hsp60, ileS, pyrG, recA and recG). The MLST method had good discriminatory ability: the 41 strains of L. delbrueckii examined were divided into 34 sequence types, with 29 sequence types represented by only a single strain. The sequence types were divided into eight groups. These groups could be discriminated as representing different subspecies. The results of the AFLP and MLST analyses were consistent. The type strain of L. delbrueckii subsp. delbrueckii, YIT 0080T, was clearly discriminated from the other strains currently classified as members of this subspecies, which were located close to strains of L. delbrueckii subsp. lactis. The MLST scheme developed in this study should be a useful tool for the identification of strains of L. delbrueckii to the subspecies level.
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Didelot, Xavier, Rachel Urwin, Martin C. J. Maiden, and Daniel Falush. "Genealogical typing of Neisseria meningitidis." Microbiology 155, no. 10 (2009): 3176–86. http://dx.doi.org/10.1099/mic.0.031534-0.

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Despite the increasing popularity of multilocus sequence typing (MLST), the most appropriate method for characterizing bacterial variation and facilitating epidemiological investigations remains a matter of debate. Here, we propose that different typing schemes should be compared on the basis of their power to infer clonal relationships and investigate the utility of sequence data for genealogical reconstruction by exploiting new statistical tools and data from 20 housekeeping loci for 93 isolates of the bacterial pathogen Neisseria meningitidis. Our analysis demonstrated that all but one of the hyperinvasive isolates established by multilocus enzyme electrophoresis and MLST were grouped into one of six genealogical lineages, each of which contained substantial variation. Due to the confounding effect of recombination, evolutionary relationships among these lineages remained unclear, even using 20 loci. Analyses of the seven loci in the standard MLST scheme using the same methods reproduced this classification, but were unable to support finer inferences concerning the relationships between the members within each complex.
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Dissertations / Theses on the topic "Multilocus sequence typing (MLST)"

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Baleela, Rania Mohamed Hasson. "Sudanese `Leishmania donovani' : population structure and recombination uncovered by multilocus microsattelite typing (MLMT), multilocus sequence typing (MLST) and DNA quantitation." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.536932.

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Thickett, Kathleen Mary. "Towards developing a multilocus sequence typing (MLST) scheme for Burkholderia cepacia complex." Thesis, University of Warwick, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.403134.

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McDonald, Kristina Marie. "Mycoplasma bovigenitalium qPCR Detection and Multilocus Sequence Typing Strain Differentiation." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1482317197896844.

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Gomes, Catarina dos Santos Teles. "Identification and phylogenetic characterization of Ralstonia solanacearum species complex isolates collected in Portugal." Master's thesis, ISA, 2017. http://hdl.handle.net/10400.5/15089.

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Matta, Daniel Archimedes da [UNIFESP]. "Caracterização de isolados clínicos de Candida albicans de estudo brasileiro multicêntrico de candidemia por metodologia de “Multilocus Sequence Typing (MLST)”." Universidade Federal de São Paulo (UNIFESP), 2009. http://repositorio.unifesp.br/handle/11600/9170.

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Made available in DSpace on 2015-07-22T20:49:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-09-30<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>A metodologia do “Multilocus Sequence Typing (MLST)” tornou-se uma ferramenta importante para tipagem molecular para C. albicans porque esta metodologia pode caracterizar grande número de isolados rapidamente e está isenta da interpretação subjetiva de padrões de bandas em géis de eletroforese. Este método é muito útil no entendimento da filogenia e epidemiologia de cepas de C. albicans recuperadas de infecções fúngicas invasivas. Objetivos: 1) aplicar as metodologias de MLST e Tipagem ABC para isolados de C. albicans recuperados de infecções de corrente sanguínea em hospitais terciários no Brasil e 2) determinar se cepas indistinguíveis ou diferentes foram responsáveis pelos episódios de candidemia persistente ou candidemia recorrente em isolados sequenciais de mesmo paciente. Material e Métodos: Nós aplicamos a metodologia do MLST e Tipagem ABC em isolados de C. albicans de 61 pacientes com candidemia coletados durante um estudo multicêntrico realizado em 11 hospitais públicos terciários de 9 cidades brasileiras. Também foram avaliados os isolados sequenciais de 8 pacientes com candemia persistente ou recorrente. Candidemia persistente foi definido como um episódio de fungemia com duas ou mais culturas positivas para C. albicans, em 2 ou mais dias diferentes, a despeito da contínua terapia antifúngica adotada. Candidemia recorrente foi definida como um episódio de candidemia ocorrendo ao menos 1 mês após o episódio incidente e a negativação de duas hemoculturas sequenciais após introdução da terapia antifúngica, envolvendo a mesma espécie de Candida. Resultados: Um total de 48 únicos “diploid sequence types (DSTs)” foram observados, incluindo 10 novos genótipos e 32 novos DSTs. DST 69 foi o mais comum entre os nossos isolados. Isolados clado 1 responderam a 56% da nossa coleção. O clado 3 e clado 8 foram os clados com maior número de isolados depois de clado 1, ambos respondendo por 10% das amostras. O clado 9 e clado 17 foram responsáveis por 6,5% dos isolados cada um. Isolados clado 12 responderam por 5%. Foi isolada uma única cepa (1,5%) do clado 2, clado 4, clado 16 e um isolado categorizado como “solitário”. Para Tipagem ABC, 82% dos isolados foram classificados como tipo A, seguido por tipo B com 16,5% e tipo C com 1,5%. Quanto aos pacientes com candidemia persistente ou recorrente, para todos os pacientes exceto um, verificou-se a permanência dos mesmos DSTs encontrados entre a primeira e última amostra coletada. Um único paciente com coletas sequenciais pelo período de 10 dias apresentou 3 cepas distintas discriminadas pelo MLST. Uma destas 3 cepas foi a única representante do clado 2 em nosso estudo. Conclusão: Mais de 50% dos isolados deste estudo apresentaram novos DSTs, predominando o clado 1 em 56% das amostras. Para a Tipagem ABC, 82% dos isolados foram do tipo A. Este é o primeiro estudo de nosso conhecimento a descrever infecção de corrente sanguínea por 3 cepas distintas de C. albicans documentadas no período de 10 dias.<br>The DNA sequence-based genotyping technique multilocus sequence typing (MLST) has emerged as an alternative typing tool for C. albicans because can characterize large numbers of isolates rapidly, and does not require the subjective interpretation of banding patterns. This methodology is a very useful tool in understanding the phylogenetics and epidemiology of C. albicans strains from invasive candidiasis. Objective: Our goal was 1) to apply MLST and ABC typing to C. albicans strains recovered from bloodstream infection from public tertiary care hospitals in Brazil and 2) determine whether indistinguishable or different strains were responsible for persistent or recurrent fungemia by performing MLST and ABC typing on sequential C. albicans isolates from the same patient. Methods: We applied MLST and ABC typing, which is based on the presence or absence of an intron in the 25S rDNA region, to C. albicans strains from 61 patients with candidemia collected during a multicenter surveillance study in 11 public tertiary care hospitals, representative of the public health system of 9 of the largest cities in Brazil. We also analyzed C. albicans strains from 8 patients with persistent or recurrent candidemia. Persistent candidemia was defined as two or more blood cultures positive for C. albicans on 2 or more separate days. Recurrent candidemia was defined as an episode of candidemia occurring at least 1 month after the apparent complete resolution of an infectious episode caused by the same Candida species. Results: A total of 48 unique profiles or diploid sequence types (DST) were observed, with 10 new sequence types (STs) and 32 new DSTs. DST 69 was the most common DST isolated. C. albicans clade 1 accounted for 56% of the collection, clade 3 and clade 8 for 10% each, clades 9 and 17 for 6.5% each, and clade 12 for 5%. Clade 2, clade 4, clade 16 and a singleton strain had 1 isolate each (1.5%). For ABC typing, 82% of the isolates were classified as type A, followed for 16.5% type B and 1.5% type C. All the patients’ strains related to persistent or recurrent candidemia but one showed the same MLST diploid sequence type (DST), ABC type and susceptibility profile to antifungals in the first and second samples. One patient with 7 samples collected sequentially over 10 days showed 3 distinct strains, well discriminated by MLST. One of the 3 strains recovered from this patient showed a single C. albicans isolate found in our total collection classified as clade 2, although clade 2 is commonly found worldwide. Conclusion: More than 50% of isolates from this study form a unique set of DSTs and clade 1 was responsible for 56% of the isolates. For ABC typing, 82% of the isolates were type A. To the best of our knowledge, this is the first study describing a blood stream infection with 3 distinct C. albicans strains in the same patient within a short period of time.<br>CNPq: GM/GD 142025-2005-4<br>CNPq: SWE 200669/2007-9<br>TEDE<br>BV UNIFESP: Teses e dissertações
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Joseph, S. M. "Diversity and virulence of the genus Cronobacter revealed by multilocus sequence typing (MLST) and comparative genomic analysis." Thesis, Nottingham Trent University, 2013. http://irep.ntu.ac.uk/id/eprint/221/.

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Cronobacter spp. (previously known as Enterobacter sakazakii) is a diverse bacterial genus consisting of opportunistic food-borne pathogens affecting all age groups, with particularly severe clinical complications such as meningitis and necrotising enterocolitis in neonates and infants. In this study, a multilocus sequence typing (MLST) approach has been established to span the entire Cronobacter genus, by employing the alleles of 7 housekeeping genes (atpD, fusA, glnS, gltB, gyrB, infB and ppsA, total length 3036 bp). The 325 Cronobacter spp. strains used in the study included isolates from the highly publicised Cronobacter cases from USA in December 2011. The scheme identified 115 sequence types (ST) across the seven Cronobacter species. Multilocus sequence analysis (MLSA) revealed considerable diversity in the genus, with intraspecific variation ranging from low diversity in C. sakazakii to extensive diversity within some species such as C. muytjensii and C. dublinensis including evidence of recombination events between species. An evolutionary analysis revealed the Cronobacter genus to have evolved 45-68 million years ago, during the period of evolution of flowering plants. The MLSA was also used in a polyphasic study for the formal recognition of two new species – C. universalis and C. condimenti. The MLST scheme also revealed the high level of clonality in the species C. sakazakii and C. malonaticus. ST4 was found to be a highly stable clone of C. sakazakii, and a strong association was established between the C. sakazakii ST4 clonal complex with neonatal meningitis cases. The curated MLST database is hosted with open access at: http://www.pubmlst.org/cronobacter. The diversity and virulence study of the organism was then extended to a whole genomic level by analysing eleven high quality draft Cronobacter spp. genomes spanning the seven species, including a representative of the C. sakazakii ST4 lineage, together with two publicly available genomes. Genome comparison revealed that pair-wise DNA sequence identity varies between 89 and 97% in the seven Cronobacter species. The number of annotated genes per genome varied between 3,700 and 4,200. The dataset revealed a pan-genome of more than 6000 genes, 32% of which was found to be conserved across the genus. Genes encoding adhesins, type six secretion systems, metal resistance genes as well as prophages were found in only subsets of genomes and have contributed considerably to the variation of genomic content. Sets of universal core genes and accessory genes unique to each species were identified, which can be used for designing genus/species specific detection assays. The C. sakazakii species was found to be unique in the Cronobacter genus in encoding genes enabling the utilization of exogenous sialic acid which may have considerable clinical significance. The C. sakazakii ST4 genome did not reveal any unique lineage-specific virulence related genes, suggesting the role of selective gene expression and increased host exposure in the pathogenicity of the organism.
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Langenwalder, Denis Benjamin [Verfasser]. "Beurteilung der Humanpathogenität von Anaplasma phagocytophilum Stämmen anhand ihrer drhm Genpositivität, Multilocus Sequence Typing (MLST) und des ankA-Genclusters / Denis Benjamin Langenwalder." Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2021. http://d-nb.info/1238223052/34.

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Christerson, Linus. "High Resolution Genotyping of Chlamydia trachomatis." Doctoral thesis, Uppsala universitet, Klinisk bakteriologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-156751.

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Chlamydia trachomatis is an obligate intracellular bacterium of major human health concern, causing urogential chlamydia infections, lymphogranuloma venereum (LGV) and trachoma. Chlamydia is one of the most common sexually transmitted infections worldwide and can cause infertility. In the first four papers described herein we used a high resolution multilocus sequence typing (MLST) system to investigate the epidemiology of C. trachomatis, and showed that MLST is superior to conventional ompA genotyping with respect to resolution. In the fifth paper we simplified the methodology by developing and validating a multilocus typing (MLT) DNA microarray based on the MLST system. In more detail, MLST analysis of consecutive specimens from 2006 in Örebro County in Sweden, and comparison to specimens from 1999-2000, showed that the new variant C. trachomatis (nvCT) is monoclonal and likely has appeared in recent years. MLST analysis of LGV specimens from men who have sex with men (MSM) showed that the increase of LGV in Europe in the last decade indeed was a clonal outbreak, contrary to the USA where LGV might have been present all along. In the third paper, clinical symptoms could not be correlated with the MLST genotypes, suggesting, together with the combined results of all previous studies, that bacterial factors, if important, need to be understood in the context of host factors. MLST analysis of specimens from a high incidence C. trachomatis area in North Norway revealed interesting epidemiological details concerning unusual genetic variants, the nvCT and MSM, but found no significant difference in genetic diversity compared to two other geographic areas in Norway. Lastly, we developed a MLT array that provides high resolution while being rapid and cost-effective, which makes it an interesting alternative for C. trachomatis genotyping. In conclusion, the MLST system and the MLT array have proven to be useful tools and should now be applied in further investigations to improve our understanding of C. trachomatis epidemiology.
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Martins, Cíntia [UNESP]. "Determinação de cepas de Wolbachia em populações naturais de Solenopsis spp. (Hymenoptera: Formicidae) analisadas via Multilocus Sequence Typing (MLST): diversidade genética, coevolução e recombinação." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/134186.

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Made available in DSpace on 2016-02-05T18:30:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-07-02. Added 1 bitstream(s) on 2016-02-05T18:34:13Z : No. of bitstreams: 1 000857534.pdf: 1229333 bytes, checksum: 4a0683950d7b91d5ae07474bfd3e39ef (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Fundação de Amparo a Pesquisa do Estado do Piauí (FAPEPI)<br>Interações insetos/micro-organismos são amplas e ocorrem das mais diversas maneiras, com as bactérias simbiontes de insetos desempenhando vários papéis, na maioria desconhecidos, na biologia do hospedeiro. O que se considerava apenas um único organismo eucariótico é na verdade um agregado de vários diferentes organismos, o que levou a uma mudança na maneira de se estudar esses organismos, culminando em uma abordagem mais holística. Dentro desse vasto mundo, relativamente pouco conhecido dos micro-organismos em associação com insetos, estão as bactérias do gênero Wolbachia (Classe Alphaproteobacteria, Ordem Rickettsiales), amplamente distribuídas nos artrópodes e transmitidas maternalmente, causadoras de diversas alterações reprodutivas no hospedeiro, sendo que sua ocorrência em populações naturais pode ser de grande interesse no controle biológico. A distribuição dessas bactérias em formigas é pouco explorada e existe carência de informações sobre a interação com formigas do gênero Solenopsis, que inclui espécies nativas da América do Sul. Este gênero possui espécies distribuídas de forma cosmopolita e no Brasil estão amplamente disseminadas, associadas preferencialmente a áreas de atividade humana. Este trabalho teve como objetivo analisar a diversidade genética da Wolbachia de amostras de ninhos de populações nativas de espécies do gênero Solenopsis, através do sequenciamento de cinco genes que compõe o multilocus de Wolbachia, além do gene wsp, a fim de caracterizar as cepas e estabelecer inferências filogenéticas entre elas. Além disso, testar as hipóteses de coevolução entre as cepas e as formigas e de recombinação entre as cepas encontradas. Com o sequenciamento e análises dos cinco genes que compõem o multilocus de Wolbachia (gatB, coxA, hcpA, ftsZ e fbpA), totalizando 2079 pb, destacam-se os seguintes resultados: i. o registro de 15 novas cepas; ii. o registro de 11 alelos...<br>Insects/microorganisms interactions are broad and occurs in many different ways with symbiont bacteria of insects playing many roles, most unknown, on the biology if its host. What was considered a single eukaryotic organism is actually an aggregate of many different organisms which lead to a change in the way we study organisms, leading to a more holistic approach. Within this vast but relatively unknown world of microorganisms in association with insects are the bacteria of the genus Wolbachia (Class Alphaproteobacteria, Order Rickettsiales) widely distributed in arthropods and maternally transmitted, causing several reproductive alterations in the host, and their occurrence in natural populations being of great interest in the biological control of insects. Its distribution in ants is poorly explored and little is known about the interaction with the Solenopsis genus which includes native species from South America. This genus includes species cosmopolitan distributed and in Brazil they are widely distributed being preferentially associated with areas of human activity. This study aimed to analyze the genetic diversity of samples of nests from native populations of Solenopsis species infected by Wolbachia by sequencing the five genes comprising the Wolbachia multilocus, and also the wsp gene in order to characterize the strains and establish phylogenetic inferences between them. Furthermore test the hypothesis of coevolution between ants and its Wolbachia strains and recombination between found strains. With the sequencing and analysis of five genes comprising the Wolbachia multilocus (gatB, coxA, hcpA, ftsZ e fbpA) totaling 2079 bp the following results are highlighted: i. the record of 15 previously unknown new strains, ii. the record of 11 previously unknown new alleles, iii. phylogenetic relationship between the strains found here presents a polyphyletic pattern, indicative of the complexity of the evolutionary history of strains ...
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Martins, Cíntia. "Determinação de cepas de Wolbachia em populações naturais de Solenopsis spp. (Hymenoptera: Formicidae) analisadas via Multilocus Sequence Typing (MLST) : diversidade genética, coevolução e recombinação /." Rio Claro, 2014. http://hdl.handle.net/11449/134186.

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Orientador: Odair Correa Bueno<br>Banca: Denise S. Scheepmaker<br>Banca: Ana Eugenia de Carvalho Campos<br>Banca: José Chaud Netto<br>Banca: Osmar Malaspina<br>Resumo: Interações insetos/micro-organismos são amplas e ocorrem das mais diversas maneiras, com as bactérias simbiontes de insetos desempenhando vários papéis, na maioria desconhecidos, na biologia do hospedeiro. O que se considerava apenas um único organismo eucariótico é na verdade um agregado de vários diferentes organismos, o que levou a uma mudança na maneira de se estudar esses organismos, culminando em uma abordagem mais holística. Dentro desse vasto mundo, relativamente pouco conhecido dos micro-organismos em associação com insetos, estão as bactérias do gênero Wolbachia (Classe Alphaproteobacteria, Ordem Rickettsiales), amplamente distribuídas nos artrópodes e transmitidas maternalmente, causadoras de diversas alterações reprodutivas no hospedeiro, sendo que sua ocorrência em populações naturais pode ser de grande interesse no controle biológico. A distribuição dessas bactérias em formigas é pouco explorada e existe carência de informações sobre a interação com formigas do gênero Solenopsis, que inclui espécies nativas da América do Sul. Este gênero possui espécies distribuídas de forma cosmopolita e no Brasil estão amplamente disseminadas, associadas preferencialmente a áreas de atividade humana. Este trabalho teve como objetivo analisar a diversidade genética da Wolbachia de amostras de ninhos de populações nativas de espécies do gênero Solenopsis, através do sequenciamento de cinco genes que compõe o multilocus de Wolbachia, além do gene wsp, a fim de caracterizar as cepas e estabelecer inferências filogenéticas entre elas. Além disso, testar as hipóteses de coevolução entre as cepas e as formigas e de recombinação entre as cepas encontradas. Com o sequenciamento e análises dos cinco genes que compõem o multilocus de Wolbachia (gatB, coxA, hcpA, ftsZ e fbpA), totalizando 2079 pb, destacam-se os seguintes resultados: i. o registro de 15 novas cepas; ii. o registro de 11 alelos...<br>Abstract: Insects/microorganisms interactions are broad and occurs in many different ways with symbiont bacteria of insects playing many roles, most unknown, on the biology if its host. What was considered a single eukaryotic organism is actually an aggregate of many different organisms which lead to a change in the way we study organisms, leading to a more holistic approach. Within this vast but relatively unknown world of microorganisms in association with insects are the bacteria of the genus Wolbachia (Class Alphaproteobacteria, Order Rickettsiales) widely distributed in arthropods and maternally transmitted, causing several reproductive alterations in the host, and their occurrence in natural populations being of great interest in the biological control of insects. Its distribution in ants is poorly explored and little is known about the interaction with the Solenopsis genus which includes native species from South America. This genus includes species cosmopolitan distributed and in Brazil they are widely distributed being preferentially associated with areas of human activity. This study aimed to analyze the genetic diversity of samples of nests from native populations of Solenopsis species infected by Wolbachia by sequencing the five genes comprising the Wolbachia multilocus, and also the wsp gene in order to characterize the strains and establish phylogenetic inferences between them. Furthermore test the hypothesis of coevolution between ants and its Wolbachia strains and recombination between found strains. With the sequencing and analysis of five genes comprising the Wolbachia multilocus (gatB, coxA, hcpA, ftsZ e fbpA) totaling 2079 bp the following results are highlighted: i. the record of 15 previously unknown new strains, ii. the record of 11 previously unknown new alleles, iii. phylogenetic relationship between the strains found here presents a polyphyletic pattern, indicative of the complexity of the evolutionary history of strains ...<br>Doutor
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Books on the topic "Multilocus sequence typing (MLST)"

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Threlfall, E. J., J. Wain, and C. Lane. Salmonellosis. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0030.

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Salmonellosis remains the second most common form of bacterial food-poisoning in the UK and in most of the developed economies. Although the number of isolations per annum has declined since 2000, over 10,000 laboratory-confirmed cases are recognised each year in England and Wales, and over 150,000 in Europe. Most of infections are associated with contaminated food, particularly of poultry origin, but also may originate from cattle and pigs, and to a lesser extent, sheep. The most common serovars from cases of human infection is Enteritidis, followed by Typhimurium. Contact with pets, particularly reptiles and amphibians is becoming an increasing problem and infections can be severe, particularly in children. Accurate and reproducible methods of identification and subtyping are crucial for meaningful epidemiological investigations, and traditional phenotypic methods of typing are now being supplemented by DNA- based methods such as pulsed-field gel electrophoresis, variable number of tandem repeats analysis, and multilocus sequence typing. The use of such methods in combination with phenotypic methods has been invaluable for outbreak control at the international level. The occurrence of resistance to antimicrobial drugs is an increasing problem, particularly in relation to the development of resistance to antimicrobials regarded as ‘critically-important’ for last resort therapy in humans. Control measures such as vaccination of poultry flocks appear to have had a substantial impact on the number of infections with Salmonella Enteritidis. Nevertheless good hygiene practices in both catering establishments and the home remain essential for the control of infections at the local level.
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Book chapters on the topic "Multilocus sequence typing (MLST)"

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Jelocnik, Martina, Adam Polkinghorne, and Yvonne Pannekoek. "Multilocus Sequence Typing (MLST) of Chlamydiales." In Chlamydia trachomatis. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9694-0_7.

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Saunders, Nicholas A., and Anne Holmes. "Multilocus Sequence Typing (MLST) of Staphylococcus aureus." In Methods in Molecular Biology. Humana Press, 2007. http://dx.doi.org/10.1007/978-1-59745-468-1_6.

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Stessl, Beatrix, Irene Rückerl, and Martin Wagner. "Multilocus Sequence Typing (MLST) of Listeria monocytogenes." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0703-8_6.

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Saunders, Nicholas A., and Anne Holmes. "Multilocus Sequence Typing (MLST) of Staphylococcus aureus." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-664-1_7.

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Joseph, Susan, and Stephen Forsythe. "Multilocus Sequence Typing (MLST) for Cronobacter spp." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7037-7_16.

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Gilchrist, Carol A. "Multilocus Sequence Typing System (MLST): Genetic Diversity and Genetic Components to Virulence." In Amebiasis. Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-55200-0_4.

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Ahmed, Ahmed, Ana S. Ferreira, and Rudy A. Hartskeerl. "Multilocus Sequence Typing (MLST): Markers for the Traceability of Pathogenic Leptospira Strains." In Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2004-4_25.

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Epping, Lennard, Esther-Maria Antão, and Torsten Semmler. "Population Biology and Comparative Genomics of Campylobacter Species." In Current Topics in Microbiology and Immunology. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-65481-8_3.

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AbstractThe zoonotic pathogen Campylobacter is the leading cause for bacterial foodborne infections in humans. Campylobacters are most commonly transmitted via the consumption of undercooked poultry meat or raw milk products. The decreasing costs of whole genome sequencing enabled large genome-based analyses of the evolution and population structure of this pathogen, as well as the development of novel high-throughput molecular typing methods. Here, we review the evolutionary development and the population diversity of the two most clinically relevant Campylobacter species; C. jejuni and C. coli. The state-of-the-art phylogenetic studies showed clustering of C. jejuni lineages into host specialists and generalists with coexisting lifestyles in chicken and livestock-associated hosts, as well as the separation of C. coli isolates of riparian origin (waterfowl, water) from C. coli isolated from clinical and farm-related samples. We will give an overview of recombination between both species and the potential impact of horizontal gene transfer on host adaptation in Campylobacter. Additionally, this review briefly places the current knowledge of the population structure of other Campylobacter species such as C. lari, C. concisus and C. upsaliensis into perspective. We also provide an overview of how molecular typing methods such as multilocus sequence typing (MLST) and whole genome MLST have been used to detect and trace Campylobacter outbreaks along the food chain.
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Stessl, Beatrix, Martin Wagner, and Werner Ruppitsch. "Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua." In Listeria Monocytogenes. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0982-8_7.

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Knabel, Stephen J. "Multilocus Sequence Typing." In DNA Methods in Food Safety. John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118278666.ch3.

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Conference papers on the topic "Multilocus sequence typing (MLST)"

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Thakur, Siddartha, and Wondwossen A. Gebreyes. "Comparative genotyping of Campylobacter coli using multilocus sequence typing and pulsed field gel electrophoresis." In Sixth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2005. http://dx.doi.org/10.31274/safepork-180809-749.

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Yan, H., Y. Yueping, L. Hongye, and C. Xiangsheng. "P3.239 High-resolution multilocus sequence typing of urogenitalchlamydia trachomatisin std clinic outpatients in two chinese provinces." In STI and HIV World Congress Abstracts, July 9–12 2017, Rio de Janeiro, Brazil. BMJ Publishing Group Ltd, 2017. http://dx.doi.org/10.1136/sextrans-2017-053264.474.

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Wang, B., C. M. Logue, K. Tivendale, James D. McKean, and Annette M. O'Connor. "Multilocus Sequence Typing for Characterization of Non typhoidal Salmonella Strains in Swine Isolated on Farm and at Slaughter." In Fourth International Symposium on the Epidemiology and Control of Salmonella and Other Food Borne Pathogens in Pork. Iowa State University, Digital Press, 2009. http://dx.doi.org/10.31274/safepork-180809-233.

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Versteeg, Bart, Sylvia Bruisten, Odile Harrison, et al. "P1.06 In silico multilocus sequence typing ofchlamydia trachomatisplasmids shows clustering of isolates according to the disease related biovars." In STI and HIV World Congress Abstracts, July 9–12 2017, Rio de Janeiro, Brazil. BMJ Publishing Group Ltd, 2017. http://dx.doi.org/10.1136/sextrans-2017-053264.114.

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