Relevant bibliographies by topics / Multimodal imaging probe

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Multimodal imaging probe'

Author: Grafiati
Published: 4 June 2021
Last updated: 11 February 2022

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Journal articles on the topic "Multimodal imaging probe"

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Hostachy, Sarah, Marie Masuda, Takayuki Miki, et al. "Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins." Chemical Science 9, no. 19 (2018): 4483–87. http://dx.doi.org/10.1039/c8sc00886h.

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Schanne, Gabrielle, Lucas Henry, How Chee Ong, et al. "Rhenium carbonyl complexes bearing methylated triphenylphosphonium cations as antibody-free mitochondria trackers for X-ray fluorescence imaging." Inorganic Chemistry Frontiers 8, no. 16 (2021): 3905–15. http://dx.doi.org/10.1039/d1qi00542a.

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Yadav, Aditya, Chethana Rao, Navneet Chandra Verma, Pushpendra Mani Mishra, and Chayan Kanti Nandi. "Magnetofluorescent Nanoprobe for Multimodal and Multicolor Bioimaging." Molecular Imaging 19 (January 1, 2020): 153601212096947. http://dx.doi.org/10.1177/1536012120969477.

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Although, superparamagnetic iron oxide nanoparticles (SPIONs) have extensively been used as a contrasting agent for magnetic resonance imaging (MRI), the lack of intrinsic fluorescence restricted their application as a multimodal probe, especially in combination with light microscopy. In Addition, the bigger size of the particle renders them incompetent for bioimaging of small organelles. Herein, we report, not only the synthesis of ultrasmall carbon containing magneto-fluorescent SPIONs with size ∼5 nm, but also demonstrate its capability as a multicolor imaging probe. Using MCF-7 and HeLa cell lines, we show that the SPIONs can provide high contrast mulicolor images of the cytoplasm from blue to red region. Further, single particle level photon count data revealed that the SPIONs could efficaciously be utilized in localization based super resolution microscopy in future.
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Bang, J. J., S. R. Russell, K. K. Rupp, and S. A. Claridge. "Multimodal scanning probe imaging: nanoscale chemical analysis from biology to renewable energy." Analytical Methods 7, no. 17 (2015): 7106–27. http://dx.doi.org/10.1039/c5ay00507h.

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Tam, Jenny, Alexander Pilozzi, Umar Mahmood, and Xudong Huang. "Simultaneous Monitoring of Multi-Enzyme Activity and Concentration in Tumor Using a Triply Labeled Fluorescent In Vivo Imaging Probe." International Journal of Molecular Sciences 21, no. 9 (2020): 3068. http://dx.doi.org/10.3390/ijms21093068.

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The use of fluorescent imaging probes that monitor the activity of proteases that experience an increase in expression and activity in tumors is well established. These probes can be conjugated to nanoparticles of iron oxide, creating a multimodal probe serving as both a magnetic resonance imaging (MRI) agent and an indicator of local protease activity. Previous works describe probes for cathepsin D (CatD) and metalloproteinase-2 (MMP2) protease activity grafted to cross-linked iron oxide nanoparticles (CLIO). Herein, we have synthesized a triply labeled fluorescent iron oxide nanoparticle molecular imaging (MI) probe, including an AF750 substrate concentration reporter along with probes for cathepsin B (CatB) sand MMP2 protease activity. The reporter provides a baseline signal from which to compare the activity of the two proteases. The activity of the MI probe was verified through incubation with the proteases and tested in vitro using the human HT29 tumor cell line and in vivo using female nude mice injected with HT29 cells. We found the MI probe had the appropriate specificity to the activity of their respective proteases, and the reporter dye did not activate when incubated in the presence of only MMP2 and CatB. Probe fluorescent activity was confirmed in vitro, and reporter signal activation was also noted. The fluorescent activity was also visible in vivo, with injected HT29 cells exhibiting fluorescence, distinguishing them from the rest of the animal. The reporter signal was also observable in vivo, which allowed the signal intensities of the protease probes to be corrected; this is a unique feature of this MI probe design.
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Li, Yan, Jason Chen, and Zhongping Chen. "Multimodal intravascular imaging technology for characterization of atherosclerosis." Journal of Innovative Optical Health Sciences 13, no. 01 (2019): 2030001. http://dx.doi.org/10.1142/s1793545820300013.

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Early detection of vulnerable plaques is the critical step in the prevention of acute coronary events. Morphology, composition, and mechanical property of a coronary artery have been demonstrated to be the key characteristics for the identification of vulnerable plaques. Several intravascular multimodal imaging technologies providing co-registered simultaneous images have been developed and applied in clinical studies to improve the characterization of atherosclerosis. In this paper, the authors review the present system and probe designs of representative intravascular multimodal techniques. In addition, the scientific innovations, potential limitations, and future directions of these technologies are also discussed.
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Kang, Won Jun, Jonghwan Lee, Yong Seung Lee, et al. "Multimodal imaging probe for targeting cancer cells using uMUC-1 aptamer." Colloids and Surfaces B: Biointerfaces 136 (December 2015): 134–40. http://dx.doi.org/10.1016/j.colsurfb.2015.09.004.

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Walia, Shanka, and Amitabha Acharya. "Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy." Beilstein Journal of Nanotechnology 6 (February 24, 2015): 546–58. http://dx.doi.org/10.3762/bjnano.6.57.

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Nano-theranostics offer remarkable potential for future biomedical technology with simultaneous applications for diagnosis and therapy of disease sites. Through smart and careful chemical modifications of the nanoparticle surface, these can be converted to multifunctional tiny objects which in turn can be used as vehicle for delivering multimodal imaging agents and therapeutic material to specific target sites in vivo. In this sense, bimodal imaging probes that simultaneously enable magnetic resonance imaging and fluorescence imaging have gained tremendous attention because disease sites can be characterized quick and precisely through synergistic multimodal imaging. But such hybrid nanocomposite materials have limitations such as low chemical stability (magnetic component) and harsh cytotoxic effects (fluorescent component) and, hence, require a biocompatible protecting agent. Silica micro/nanospheres have shown promise as protecting agent due to the high stability and low toxicity. This review will cover a full description of MRI-active and fluorescent multifunctional silica micro/nanospheres including the design of the probe, different characterization methods and their application in imaging and treatment in cancer.
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Moon, Sung-Hyun, Bo Yeun Yang, Yun-Sang Lee, Dong Soo Lee, June-Key Chung, and Jae Min Jeong. "Development of a PSMA targeting nanoparticle as PET/MR multimodal imaging probe." Nuclear Medicine and Biology 41, no. 7 (2014): 630. http://dx.doi.org/10.1016/j.nucmedbio.2014.05.023.

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Kang, Nam-Young, Jung Yeol Lee, Sang Hee Lee та ін. "Multimodal Imaging Probe Development for Pancreatic β Cells: From Fluorescence to PET". Journal of the American Chemical Society 142, № 7 (2020): 3430–39. http://dx.doi.org/10.1021/jacs.9b11173.

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Dissertations / Theses on the topic "Multimodal imaging probe"

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Tran, Vu Long. "Synthesis, Functionalization and Characterization of Ultrasmall Hybrid Silica Nanoparticles for Theranostic Applications." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1020/document.

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Les nanoparticules (NPs) hybrides peuvent combiner les propriétés physiques uniques des éléments inorganiques pour des applications en imagerie et en thérapeutique avec la biocompatibilité des structures organiques. Cependant, leur utilisation en médecine est encore limitée par des risques potentiels de toxicité à long terme. Dans ce contexte, des NPs hybrides ultrafines pouvant être éliminées rapidement par la voie rénale apparaissent comme de bonnes candidates pour la nanomédicine. La NP à base de silice contenant des chélates du gadolinium appelée AGuIX (Activation et Guidage de l’Irradiation par rayon-X) a été développée avec un diamètre hydrodynamique de moins de 5 nm qui lui permet d’être éliminée rapidement via l’urine après injection intraveineuse. Cette NP s’est révélée être une sonde efficace en imagerie multimodale et un amplificateur local en radiothérapie pour le diagnostic et le traitement du cancer. Elle est en train d’être évaluée dans un essai clinique de phase I par radiothérapie des métastases cérébrales (NANO-RAD, NCT02820454). Néanmoins, la synthèse d’AGuIX est un procédé multi-étapes qui est difficilement modulable.Ce manuscrit rapporte, pour la première fois, le développement d’un protocole « one-pot » direct pour des nanoparticules de silice ultrafines (USNP) contenant des chélateurs complexés ou non à partir des précurseurs silanes chélatants moléculaires. Dans ce nouveau protocole, la taille des particules et les types des métaux chélatés peuvent être contrôlés facilement. Certaines des propriétés chimiques des USNP ont été clarifiées davantage pendant ce travail exploratoire. Les particules élaborées ont été caractérisées par différentes techniques analytiques complémentaires. Ces nouvelles nanoparticules USNPs présentent des caractéristiques similaires aux AGuIX en terms de propriétes biologiques et de biodistribution.Dans un second temps, un nouveau protocole de fonctionnalisation d’USNP par des précurseurs silanes chélatants a été développé. Ces chélatants libres fonctionnalisés sur la particule peuvent être alors utilisés afin de complexer des radiométaux pour l’imagerie bimodale. Enfin, d’autres stratégies de fonctionnalisation sont aussi décrites. La nouvelle sonde (17VTh031) combinant un petit chélateur cyclique (NODA) et un fluorophore proche-infrarouge tumeur ciblant (IR783) ainsi que le pyridinium quaternaire ont été greffés sur l’AGuIX pour créer une nouvelle sonde en imagerie multimodale et cibler des tumeurs chondrosarcomes respectivement<br>Hybrid nanoparticles (NPs) can combine unique physical properties for imaging and therapeutic applications of inorganic elements in bio-friendly organic structures. However, their uses in medicine are limited by the potential risks of long-term toxicities. In this context, ultrasmall renal clearable NPs appear as novel solutions. Silica based NP displaying gadolinium chelates named AGuIX (Activation and Guidance for Irradiation by X-ray) has been developed to have hydrodynamic diameter less than 5 nm which allows rapid elimination through urine after intravenous injection. This NP has been demonstrated as an efficient multimodal imaging probe and a local enhancer for radiotherapy for cancer diagnostics and treatment. It is now being evaluated in a phase I clinical trial by radiotherapy of cerebral metastases (NANO-RAD NCT02820454). Nevertheless, the synthesis of AGuIX implies a multisteps process that can be further improved.This manuscript shows, for the first time, the development of a straightforward one-pot protocol for ultrasmall silica nanoparticles (USNP) containing complexed or non-complexed chelators from molecular chelating silane precursors. In this new protocol, the size of particle and types of metals can be easily tuned. The chemical properties of USNP have been further clarified during this exploratory work. The produced particles have been characterized by different complimentary analytical techniques. These new nanoparticles USNPs show similar characteristics to AGuIX in terms of biological properties and biodistribution.Secondly, a new protocol of functionalization for USNP by chelating silane precursors has been developed. These functionalized free chelators on the particle can be used then to complex radiometals for bimodal imaging applications. Finally, other functionalization strategies have also been described. New probe (17VTh031) combining small cyclic chelator (NODA) and tumor targeting near-infrared fluorophore (IR783) as well as quaternary pyridinium have been grafted on AGuIX for creating new multimodal imaging probe and targeting chondrosarcoma tumors respectively
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Clède, Sylvain. "From metal carbonyls to single core multimodal probes for imaging." Paris 6, 2013. http://www.theses.fr/2013PA066576.

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Les métaux carbonyles, de par leurs propriétés vibrationnelle et de luminescence, sont des candidats idéaux afin de réaliser une imagerie bimodale cellulaire. Le fil conducteur de cette thèse a été la mise au point, l’étude et l’utilisation d’un cœur rhénium tris-carbonyle [LRe(CO)3X] (L = pyridyl-1,2,3-triazole et X = Cl-) dit SCoMPI pour « Single Core Multimodal Probe for Imaging », combinant les modalités IR et de luminescence sur une structure moléculaire unique, stable en milieu biologique. Des cartes IR et de luminescence ont été réalisées sur des cellules incubées avec un dérivé SCoMPI. Leur correspondance a démontré l’intégrité en milieu cellulaire et la pertinence en tant qu’agent bimodal d’une telle unité. Il était alors possible de l’envisager comme une plateforme de spectroscopie corrélative greffable. Une première application à la détection d’un dérivé œstrogénique a mis en évidence tout son potentiel pour le traçage de molécules. L’étude approfondie de l’influence de substituants du ligand L sur la pénétration cellulaire de sondes SCoMPI a permis de mieux comprendre les paramètres influant sur leur internalisation. Le suivi IR/luminescence en temps réel de sa translocation a montré l’intérêt de ces objets pour l’imagerie de cellules vivantes. Parallèlement à ces travaux, nous avons confirmé qu’il est pertinent de considérer des rapports de bandes IR en vue de détecter des organites, sans recours à un marquage exogène. Grâce à ses nombreux atouts (faible énergie mise en jeu, pénétration accrue, signature propre à chaque fonction chimique), la spectroscopie IR est amenée à être davantage utilisée pour la résolution de problématiques biologiques<br>Metal carbonyls are ideal candidates for bimodal bio-imaging due to their appropriate vibrational and luminescence properties. The main focus of this work has been the development, the study and the use of a rhenium tris-carbonyl unit [LRe(CO)3X] (L = pyridyl-1,2,3-triazole, X = Cl-), named SCoMPI for “Single Core Multimodal Probe for Imaging”, combining IR and luminescent modalities on a unique molecular moiety, robust in biological media. Since IR and luminescent sub-cellular mappings of the first SCoMPI compound were consistent, its integrity and relevance as a bimodal imaging agent were demonstrated. A first bio-application to track an estrogen derivative showed that SCoMPIs have a great potential as luminescent and vibrational tags. The in-depth study of the influence of pendant groups on its cellular uptake allowed for a better understanding of the parameters involved. Successful bimodal live-cell imaging highlighted that SCoMPIs could be used as imaging agents in living cells. In parallel, the analysis of minute changes in intracellular spectra by synchrotron-based IR spectromicroscopy confirmed the relevance of considering specific IR band-ratios to detect organelles, with no need of exogenous staining. Thanks to its multiple assets (low energy involved, deep penetration, specific signature of each chemical function), the IR spectroscopy tends to be more used in the future for biological applications
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Li, Zizhen. "Synthesizing Multimodal Imaging Probes and Their Application in Non-Invasive Axonal Tracing by Magnetic Resonance Imaging." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34414.

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Imaging techniques have become much more in demand in modern medicine, especially in fields of disease prognosis, diagnosis and therapeutics. This is because a better understanding of different diseases, characteristics of each patient and further optimizing treatment planning, are all enhanced by advanced imaging techniques. Since each imaging modality has its own merits and intrinsic limitations, combining two or more complementary imaging modalities has become an interesting research area. In this study, gadolinium (Gd3+) doped CdTe quantum dots (QDs) were synthesized and used as multimodal imaging probes of two highly complementary imaging modalities: optical imaging and magnetic resonance imaging. The new imaging probes were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), UV-vis absorbance spectra, fluorescence spectra (FL) and magnetic resonance imaging (MRI). The optical / MRI imaging probes were further functionalized by conjugating with the axonal tracer dextran amine (10 kDa) for non-invasive axonal tracing observations. Biocompatibility and MRI contrast effect of prepared multimodal imaging probes were investigated by in vitro cell experiments and MRI scanner. Ultimately, it is hoped that this imaging probe will help us better understand the regeneration mechanisms in real time without sacrificing animals at intervening time-points.
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Vorng, Jean-luc. "Synthèse de sondes moléculaires pour l'imagerie multimodale et multi-échelle appliquée en science du vivant." Thesis, Rennes 1, 2013. http://www.theses.fr/2013REN1S123/document.

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Les travaux décrits dans ce mémoire ont pour objectif d'imager des cellules par des techniques d'imagerie complémentaires à la spectroscopie de fluorescence : la micro-spectroscopie Raman et l'imagerie NanoSIMS. Le présent manuscrit se divise en quatre chapitres et présente les différents aspects menant à la mise en place de ce projet. Le premier chapitre du manuscrit a pour but de positionner le projet d'un point de vue biologique. Ce chapitre présente l'organite que nous souhaitions marquer, le nucléole, et la façon dont nous avons procédé à sa localisation par l'intermédiaire de sondes moléculaires. Le marquage que nous avons choisi est un immuno-marquage indirect faisant intervenir deux anticorps : un anticorps primaire spécifique à la nucléophosmine, protéine majoritairement présente dans le nucléole et un anticorps secondaire, reconnaissant l'anticorps primaire, marqué par une sonde moléculaire d'intérêt. Ce chapitre a également permis de montrer par une analyse en immunofluorescence l'absence d'interférence des sondes moléculaires dans le cadre de ce marquage. Le second chapitre se focalise sur la synthèse des sondes moléculaires permettant le marquage des anticorps secondaires. Les sondes envisagées pour ce marquage possèdent un groupement ester de succinimide réactif capable de "s'accrocher" de manière covalente aux fonctions amines de l'anticorps secondaire et un ou plusieurs groupements fonctionnels visible par la technique d'imagerie voulue. Trois catégories de sondes ont été préparées et dépendent de la technique d'imagerie employée. Les sondes Raman comportant une triple liaison carbone-carbone visible par cette technique, les sondes NanoSIMS dont l'acquisition d'image sera possible par les halogènes présents dans la structure et les sondes bimodales comportant les deux éléments dans la même structure. Les sondes bimodales sont obtenues par le couplage pallado-catalysé de Sonogashira. Dans la dernière partie du chapitre, une nouvelle série de sonde a été envisagée dans le cadre d'une application future : le marquage de l'ATP-ϒ-SH grâce à une fonction mésylate. Les deux derniers chapitres ont pour but de mettre en application tout ce qui a été présenté dans les chapitres précédents. Les deux techniques employées permettent d'accéder à des types d'informations différentes : la spectroscopie Raman donne accès aux modes de vibrations d'une molécule tandis que l'imagerie NanoSIMS permet d'obtenir des informations élémentaires et isotopiques. Nous présenterons le cheminement suivi pour imager des cellules via leurs constituants par micro-spectroscopie Raman et imagerie NanoSIMS via les sondes moléculaires introduites<br>Life sciences imaging are widely used for different applications, they are interested in medical diagnosis as well as basic research. In cells biology, fluorescence microscopy is mainly used for organelles observation at sub-cellular scale. However, techniques based on fluorescence phenomena are limited by some drawbacks like technical resolution, fluorescent dye degradation and the number of channels, which can be visualized. In this context, the exploration and the development of new way for image acquisition are considered as an experimental and technical scientific challenge. Furthermore, it can lead to complementary technique to fluorescence microscopy.This PhD thesis is a life science imaging project development and application allowing image acquisition base on molecular vibrations phenomena and elementary analysis in cells. Two techniques have been chosen in relation to both specificity: micro Raman spectroscopy and NanoSIMS imaging. Micro Raman spectroscopy allows the observation of molecular vibration mode at micron scale and NanoSIMS leads to elementary and isotopic sample information at sub-micronic scale. Combination of both techniques will lead to multi-scale and multi-modal imaging of biological samples. Molecular probes designing and synthesis for both techniques were used to visualize an organelle inside the nucleus: the nucleolus. Nucleolus has a key role in ribosomal RNA transcription and researchers shows some interest in the study of this organelle for his multifunctional role like ribosome biogenesis and nuclear organization. An immuno-labelling method combine with the introduction of molecular probes will allow nucleolus imaging by micro-Raman spectroscopy and NanoSIMS spectrometry. This immuno-labelling is specific to a phosphoprotein mainly localized inside the nucleolus: the nucleophosmin (NPM). In this project, the introduction of molecular probes in an immuno-labelling will act as a Raman Tag or a NanoSIMS tag for NPM's nucleolus observation and studies.This work at the interface between different fields: chemistry, biology and physics shows all the aspect of this project starting from molecular probes synthesis, immuno-labelling methods uses to direct application of both Raman and NanoSIMS techniques
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Travaillot, Thomas. "Caractérisation mécanique des matériaux élastiques à l'échelle locale par microscopie à pointe vibrante : Approche multimodale et mesure de champs." Thesis, Besançon, 2014. http://www.theses.fr/2014BESA2011/document.

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Ces travaux de thèse proposent une amélioration du Scanning Microdeformation Microscope (SMM),un microscope à sonde locale, pour la caractérisation mécanique élastique des matériaux à l’échellelocale. Il est montré qu’en utilisant n &gt; 2 modes de résonance du SMM, il est possible de découplerles mesures du module de Young et du coefficient de Poisson d’un matériau isotrope.Une étude des modes du résonateur a permis d’enrichir son modèle afin qu’il puisse modélisern &gt; 2 modes. Des procédures ont été développées pour identifier les paramètres de ce modèle etles constantes élastiques des matériaux à partir de n &gt; 2 fréquences de résonance. Enfin, ces procéduresont été appliquées à des exemples de caractérisation à l’échelle locale afin de valider laméthode et d’en exhiber les possibilités et les limites.Pour gagner en robustesse et se diriger vers la caractérisation des matériaux anisotropes, un systèmed’imagerie interférométrique en lumière polarisée, permettant la mesure du champ de rotationde surfaces réfléchissantes dans une direction particulière, a été développé pour être intégré auSMM. Son prisme biréfringent à gradient uniaxial d’indice lui confère sa sensibilité à la rotation. Cesystème est capable de mesurer un champ de rotation localisé comme c’est le cas au voisinage dela pointe du SMM. Il a aussi montré son intérêt dans les cas où l’effet d’échelle rend particulièrementintéressante la mesure de la rotation<br>This work proposes an improvement of the Scanning Microdeformation Microscope (SMM), a scanningprobe microscope, for the mechanical elastic characterization of materials at local scale. It demonstratesthat using n &gt; 2 SMM resonance frequencies allows to decouple Young’s modulus andPoisson’s ratio values for an isotropic material.The mechanical description of the resonator has been enriched in order to allow for an accuratemodeling over a wide frequency range. Procedures have been developed to identify the modellingparameters and the elastic constants of the materials from n &gt; 2 resonant frequencies. Finally, theseprocedures have been applied to the characterization of various materials at local scale in order tovalidate the method and to present possibilities and limits.To improve robustness and move towards the characterization of anisotropic materials, a polarizedlightimaging interferometer was developed to measure the rotation field of reflecting surfaces in aparticular direction. The sensitivity to the rotation originates from a homemade birefringent prism withuniaxial gradient of refractive index. This system is able to measure a localized rotation field as it isinduced in the vicinity of the tip of the SMM. Its interest is also demonstrated in cases in which scaleeffects make the rotation measurement preferable to the out-of-plane displacement measurement
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Paris, Jérémy. "Nanoparticules d'oxydes de fer et nanotubes de titanate pour l'imagerie multimodale et à destination de la thérapie anticancéreuse." Thesis, Dijon, 2013. http://www.theses.fr/2013DIJOS065/document.

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Les possibilités offertes par les applications en médecine des nanoparticules sont l’un des facteurs essentiels des progrès médicaux attendus pour ce XXIème siècle. Ainsi, le domaine de l’imagerie médicale est aussi touché par cette évolution technologique. Ce présent travail a consisté à élaborer des sondes théranostiques à base de nanoparticules d’oxydes de fer (SPIO) et de nanotubes de titanate (TiONts) pour l’imagerie multimodale (magnétique/nucléaire ou magnétique/optique) et possédant aussi un effet thérapeutique (hyperthermie/PDT ou radiosensibilisation/PDT).Les nanotubes de titanate de cette étude, d’une longueur moyenne d’environ 150 nm, ont été obtenus par synthèse hydrothermale selon la méthode de Kasuga. Ces nanotubes présentent un diamètre extérieur de l’ordre de 10 nm et une cavité interne de 4 nm. Les nanoparticules d’oxydes de fer ont quant à elles été synthétisées par méthode de co-précipitation "Massart". Ces nanoparticules d’oxydes de fer de structure spinelle possèdent des cristallites de 9 nm de diamètre et présentent un comportement superparamagnétique mis en évidence par des mesures FC/ZFC. Pour préparer ces nanoparticules à recevoir des molécules d’intérêt biologique, deux ligands possédant des fonctions organiques plus réactives (APTES : NH2 et PHA : COOH) ont été greffés à la surface de ces deux types de nanoparticules. La présence de l'un ou l'autre a été mise en évidence par différentes techniques d’analyses (XPS, IR, zêtamétrie). La quantité de molécules greffées a été déterminé par ATG, elle est dans tous les cas d’environ 5 molécules/nm2. Dans un premier temps, les nanotubes de titanate ont été fonctionnalisés par un agent macrocyclique (0,2 DOTA/nm2). Après radiomarquage à l’indium 111, les TiONts – DOTA[In] ont été injectés dans des souris Swiss mâle nude pour connaître leur biodistribution en imagerie SPECT/CT. Les images obtenues et le comptage de la radioactivité dans chaque organe ont montré qu’au bout d’une heure, les nanotubes se situent dans les poumons et dans l’urine. Ensuite, les nanotubes sont progressivement éliminés pour n’être plus présents que dans les urines à 24 heures. Ces mêmes agents chélatants ont été greffés à la surface des SPIO pour la création de sondes multimodales IRM/SPECT ou IRM/TEP. En parallèle de cette étude, un fluorophore (phtalocyanine de zinc, ZnPc) a été greffé à la surface des nanoparticules. Le nanohybride SPIO – Pc synthétisé possède les propriétés requises pour être une sonde utilisable en imagerie bimodale IRM/IO grâce à sa longueur d’émission vers 670 nm et sa relaxivité de l’ordre de 70 L.mmolFe3O4-1.s-1. De plus, les nanohybrides ont été fonctionnalisés par du PEG pour les rendre furtifs, biocompatibles et stables. La toxicité de certains de ces nanohybrides a été évaluée avec le modèle in vivo zebrafish. Les nanohybrides étudiés n’ont pas présenté de toxicité, n’ont pas perturbé l’éclosion et n’ont pas provoqué de malformations sur les larves des zebrafish<br>The new implementations of nanoparticles in the medical field are one of the essential factors of the medical progress expected at the beginning of this XXIst century. Thus, the domain of the medical imaging is also affected by this technological evolution. This work consisted in developing theranostic probes with iron oxides nanoparticles (SPIO) and titanate nanotubes (TiONts) for multimodal imaging (magnetic/nuclear or magnetic/optical) and also possessing a therapeutic effect (hyperthermia/PDT or radiosensitization/PDT).The titanate nanotubes of this study have an average length of about 150 nm and were obtained by Kasuga's hydrothermal synthesis. These nanotubes present an outside diameter of about 10 nm and an intern cavity of 4 nm. On the other hand, iron oxides nanoparticles were synthesized by soft chemistry ("Massart" method). These spinel-like iron oxides nanoparticles have a crystallite size of 9 nm in diameter and exhibit a superparamagnetic behavior which was highlighted by FC / ZFC measurements.To get these nanoparticles ready to receive molecules of biological interest, two linkers of more reactive organic functions (APTES: NH2 or PHA: COOH) were grafted to the surface of these two types of nanoparticles. Their presence was shown by different techniques (XPS, IR, UV-vis). The amount of grafted linkers was determined by TGA and in all cases this amount is close to 5 molecules/nm2. First, titanate nanotubes were coated by a macrocyclic chelating agent (0.2 DOTA/nm2). After radiolabelling with indium 111, the TiONts – DOTA[In] nanohybrids were injected in Swiss nude mice and observed by SPECT/CT imaging to characterize their biodistribution. The SPECT/CT images and the radioactivity measured in each organ showed that after one hour, nanotubes are located in lungs and in urine. Then, the nanotubes are gradually eliminated and are only found in urines after 24 hours. The same macrocyclic agent was grafted to the SPIO’s surface for the creation of multimodal probes MRI/SPECT or MRI/PET. Alongside this study, a fluorophore (Zinc phthalocyanine) was also grafted to the surface of nanoparticles. The synthesized SPIO – Pc nanohybrid has the required properties of bimodal imaging MRI/OI probe thanks to his emission wavelength around 670 nm and its relaxivity is about 70 L.mmolFe3O4-1.s-1. Furthermore, nanohybrids were coated by PEG to make them stealth, biocompatible and stable.In this study, the toxicity of most nanohybrids was evaluated by the in vivo zebrafish model. The studied nanohybrids did not present any toxicity, hatching disruption or malformation on zebrafish larvae
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Martins, Andre Ferreira. "Multimodal Imaging Probes for the Diagnostics of Alzheimer’s Disease." Doctoral thesis, 2013. http://hdl.handle.net/10316/23523.

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Tese de doutoramento em Bioquímica, na especialidade de Biofísica Molecular, apresentada à Faculdade de Ciências e Tecnologia da Universidade de Coimbra<br>Alzheimer’s disease (AD) is the most frequent form of intellectual deterioration in elderly individuals, characterized by the brain deposition of amyloid plaques and neurofibrillary tangles. Early detection of the β-amyloid (Aβ) deposits in vivo is very difficult. Recently 11C-radiolabeled small-molecules have been developed, capable of entering the brain and specifically targeting amyloid plaques for imaging with PET, such as several Thioflavin T derivatives. In particular, the uncharged analogue 6-OH-BTA-1 (Pittsburgh compound B- PiB) is highly efficient both in crossing the BBB and in selective binding to AD amyloid aggregates. The use of Aβ marker linked to a MRI CA would constitute an attractive noninvasive in vivo imaging approach.<br>FCT - SFRH/BD/46370/2008 e CNRS - Orléans
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Book chapters on the topic "Multimodal imaging probe"

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Eckermann, Amanda L., Daniel J. Mastarone, and Thomas J. Meade. "Chemical Strategies for the Development of Multimodal Imaging Probes Using Nanoparticles." In The Chemistry of Molecular Imaging. John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118854754.ch16.

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Salmon, E. D., and J. C. Waters. "A High Resolution Multimode Digital Imaging System for Fluorescence Studies of Mitosis." In Analytical Use of Fluorescent Probes in Oncology. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4615-5845-3_37.

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"Bio-Mediated Synthesis of Quantum Dots for Fluorescent Biosensing and Bio-Imaging Applications." In Materials Research Foundations. Materials Research Forum LLC, 2021. http://dx.doi.org/10.21741/9781644901571-7.

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Quantum dots (QDs) have received great attention for development of novel fluorescent nanoprobe with tunable colors towards the near-infrared (NIR) region because of their unique optical and electronic properties such as luminescence characteristics, wide range, continuous absorption spectra and narrow emission spectra with high light stability. Quantum dots are promising materials for biosensing and single molecular bio-imaging application due to their excellent photophysical properties such as strong brightness and resistance to photobleaching. However, the use of quantum dots in biomedical applications is limited due to their toxicity. Recently, the development of novel and safe alternative method, the biomediated greener approach is one of the best aspects for synthesis of quantum dots. In this Chapter, biomediated synthesis of quantum dots by living organisms and biomimetic systems were highlighted. Quantum dots based fluorescent probes utilizing resonance energy transfer (RET), especially Förster resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET) and chemiluminescence resonance energy transfer (CRET) to probe biological phenomena were discussed. In addition, quantum dot nanocomposites are promising ultrasensitive bioimaging probe for in vivo multicolor, multimodal, multiplex and NIR deep tissue imaging. Finally, this chapter provides a conclusion with future perspectives of this field.
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Fokong, Stanley, Jabadurai Jayapaul, and Fabian Kiessling. "Multimodal Optical Imaging Probes." In Comprehensive Biomedical Physics. Elsevier, 2014. http://dx.doi.org/10.1016/b978-0-444-53632-7.00407-x.

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Conference papers on the topic "Multimodal imaging probe"

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Andreana, Marco, Tuan Le, Wolfgang Drexler, and Angelika Unterhuber. "Multimodal imaging fiber probe for biomedical applications (Conference Presentation)." In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVIII, edited by Peter R. Herman, Michel Meunier, and Roberto Osellame. SPIE, 2018. http://dx.doi.org/10.1117/12.2289530.

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Xu, Yingshun, Naitao Xu, Yanju Yang, et al. "Miniaturized Scanning Optical Probe for Multimodal Nonlinear Endomicroscopic Imaging." In 2018 International Conference on Optical MEMS and Nanophotonics (OMN). IEEE, 2018. http://dx.doi.org/10.1109/omn.2018.8454609.

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Choi, Minseog, Seungwan Lee, Jong-hyeon Chang, Eunsung Lee, Kyu-Dong Jung, and Woonbae Kim. "Design of an optical probe compatible for multimodal imaging." In SPIE Optical Engineering + Applications, edited by G. Groot Gregory and Arthur J. Davis. SPIE, 2012. http://dx.doi.org/10.1117/12.929477.

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Baria, Enrico, Enrico Pracucci, Vinoshene Pillai, Francesco S. Pavone, Gian M. Ratto, and Riccardo Cicchi. "In vivo detection of glioblastoma through multimodal fibre-probe spectroscopy." In Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, edited by Robert R. Alfano, Stavros G. Demos, and Angela B. Seddon. SPIE, 2020. http://dx.doi.org/10.1117/12.2546124.

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Li, Jiawen, Robert A. McLaughlin, Bryden C. Quirk, et al. "Imaging Genetically-Modified Cells with a Miniaturised Multimodal Optical Coherence Tomography + Fluorescence Probe." In 2019 IEEE Photonics Conference (IPC). IEEE, 2019. http://dx.doi.org/10.1109/ipcon.2019.8908410.

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Sadat Kariman, Behjat. "Multimodal and Label-Free Super-Resolution Imaging By Means of Pump-Probe Microscopy." In European Light Microscopy Initiative 2021. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.elmi2021.55.

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Fatakdawala, Hussain, FeiFei Zhou, Yang Sun, et al. "In vivo Imaging of Oral Cancer using a Multimodal Probe combining Fluorescence Lifetime, Photoacoustic and Ultrasound techniques." In Bio-Optics: Design and Application. OSA, 2013. http://dx.doi.org/10.1364/boda.2013.bm2a.3.

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Mundo, Ariel I., Gage J. Greening, and Timothy J. Muldoon. "Characterization of a multimodal endoscopically deployable veterinary spectroscopy and imaging probe to determine therapeutic response in a murine orthotopic tumor model." In Label-free Biomedical Imaging and Sensing (LBIS) 2019, edited by Natan T. Shaked and Oliver Hayden. SPIE, 2019. http://dx.doi.org/10.1117/12.2507485.

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Drake, Tyler K., Jennifer Peters, Marcus Henderson, Michael DeSoto, David Katz, and Adam Wax. "Multimodal Optical Detection of Intravaginal Microbicide Gel Coating Thickness Distribution." In Optical Molecular Probes, Imaging and Drug Delivery. OSA, 2011. http://dx.doi.org/10.1364/omp.2011.otub5.

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Pande, Paritosh, Sebina Shrestha, Jesung Park, et al. "Multimodal Optical Imaging Approach for In-Vivo Diagnosis of Oral Cancer." In Optical Molecular Probes, Imaging and Drug Delivery. OSA, 2013. http://dx.doi.org/10.1364/omp.2013.mt3c.2.

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