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Journal articles on the topic 'Multiple shoot induction'

Author: Grafiati

Published: 3 June 2025

Last updated: 19 July 2025

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1

Nisayan, E., C. Thepsithar, and A. Thongpukdee. "MULTIPLE SHOOT INDUCTION FROM SEED DERIVED SHOOTS OF PAPHIOPEDILUM." Acta Horticulturae, no. 878 (October 2010): 199–204. http://dx.doi.org/10.17660/actahortic.2010.878.23.

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2

Hasan, M. F., and B. Sikdar. "In vitro Propagation of Polygonum hydropiper L. from Shoot Tips." Plant Tissue Culture and Biotechnology 20, no. 1 (2010): 73–79. http://dx.doi.org/10.3329/ptcb.v20i1.5970.

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An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted

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3

Mangena, Phetole, Phatlane William Mokwala, and Roumiana Vassileva Nikolova. "In vitro Multiple Shoot Induction in Soybean." International Journal of Agriculture and Biology 17, no. 4 (2015): 838–42. http://dx.doi.org/10.17957/ijab/14.0006.

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4

Ugandhar, T. R., D. Sammaiah, Ramulu Ch Ayodhya, Devi U. Anitha, and P. Balaraju. "Direct multiple shoots proliferation of black night shade (Solanum nigrum l.) from shoot tip explants induced by thidiazuron." Indian Journal of Pharmaceutical and Biological Research 3, no. 01 (2015): 71–76. http://dx.doi.org/10.30750/ijpbr.3.1.11.

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The present investigation was undertaken in Black Night Shade (Solanum nigrum L.) which is an important medicinal plant. Direct multiple shoots proliferation was achieved from shoot tip. The shoot tips were cultured on MS medium fortified with Thidiazuron (TDZ) (1.0-7.0 mg/L) for multiple shoot induction. Multiple shoots proliferation was best observed at 3.0 mg/L TDZ from the shoot tip explants within three weeks of culture. Shoot number per explant ranged between 2 and 10. Individual shoots were aseptically excised and sub cultured in the same media for shoot elongation. The elongated shoots

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5

Sujatha, G., N. Jayabalan, and D. Ranjitha Kumari B. "Rapid in vitro micropropagation of Cicer arietinum L." Horticultural Science 34, No. 1 (2008): 1–5. http://dx.doi.org/10.17221/1842-hortsci.

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A rapid, simple and efficient protocol for <i>in vitro</i> multiple shoot induction and plantlet regeneration was achieved from three different explants of <i>Cicer arietinum</i>. The explants viz shoot tip, cotyledonary node and node were cultured on MS medium fortified with Benzyl Adenine (BA) (0.44&minus;8.88&mu;M) for multiple shoot induction. Multiple shoots proliferation was best observed at 4.44&mu;M BA from all the three explants within two weeks of culture. Of the three different explants tested, cotyledonary nodes produced the maximum number of sho

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6

Venkateshwarlu, Mandaloju. "MULTIPLE SHOOT INDUCTION FROM SHOOT TIP EXPLANTS OF ZIZYPHUS MAURITIANA. L." International Journal of Advanced Research 8, no. 8 (2020): 642–45. http://dx.doi.org/10.21474/ijar01/11537.

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7

Mani, N. Sarada, and Sudhakar R. Pola. "Multiple Shoot Induction from Immature Inflorescence in Sorghum." CYTOLOGIA 68, no. 2 (2003): 199–204. http://dx.doi.org/10.1508/cytologia.68.199.

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8

Khan, S., TA Banu, S. Akter, et al. "In vitro regeneration protocol of Rauvolfia serpentina L." Bangladesh Journal of Scientific and Industrial Research 53, no. 2 (2018): 133–38. http://dx.doi.org/10.3329/bjsir.v53i2.36674.

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An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of

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9

NAKAMURA, Kentaro, Yohichi WAKITA, Shinso YOKOTA, Nobuo YOSHIZAWA, and Toshinaga IDEI. "Induction of Multiple shoots by Shoot Apex Culture in Magnolia obovata Thunb." Plant tissue culture letters 12, no. 1 (1995): 34–40. http://dx.doi.org/10.5511/plantbiotechnology1984.12.34.

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10

Köse, Merve Şifa Hane, Muhammet Doğan, and Gökhan Sadi. "Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze." Eurasian Journal of Biological and Chemical Sciences 3, no. 2 (2020): 106–9. http://dx.doi.org/10.46239/ejbcs.728152.

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Staurogyne repens (Nees) Kuntze is an aquatic plant belonging to Acanthaceae family. It is a valuable plant in the aquarium industry. Therefore, there has been a significant demand for this plant. This study was designed for multiple and efficient productions of S. repens under in vitro conditions. He used nodal meristems as the type of explant. Nodal meristems were preferred as explant type. The explants were transferred to Murashige and Skoog (MS) food media with TDZ single (0-1.50 mg/L) and TDZ (0.25-1.50 mg/L) + IAA (0.25 mg/L) combinations. In TDZ application, the most number of shoots pe

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11

Kanyand, Matand, Shoemake Meordrick, and Li Chenxin. "In vitro daylily (Hemerocallis species) bract multiple shoot induction." African Journal of Biotechnology 20, no. 2 (2021): 43–50. http://dx.doi.org/10.5897/ajb2020.17281.

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12

Zhang, Mengge, Yulong Jiang, Huaqiang Zhao, and Lili Song. "Micropropagation via multiple shoot induction in Emmenopterys henryi Oliv." European Journal of Horticultural Science 84, no. 6 (2019): 336–72. http://dx.doi.org/10.17660/ejhs.2019/84.6.6.

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13

Bommineni, V. R., H. Mathews, S. B. Samuel, M. Kramer, and D. R. Wagner. "A New Method for Rapid In Vitro Propagation of Apple and Pear." HortScience 36, no. 6 (2001): 1102–6. http://dx.doi.org/10.21273/hortsci.36.6.1102.

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Improved in vitro clonal propagation methods are valuable tools for nurseries and growers, and are essential for manipulation and improvement of tree fruit germplasm using the tools and techniques of biotechnology. We have developed a rapid shoot multiplication procedure for clonal propagation of apple, Malus ×domestica cv. Gale Gala and pear, Pyrus communis L. cv. Bartlett. Rapid clonal multiplication was achieved after the following series of steps: pre-conditioning of micropropagated shoots, sectioning pre-treated stems into thin slices, placing slices onto shoot induction medium and incuba

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14

Kausar, M., S. Parvin, ME Haque, M. Khalekuzzaman, B. Sikdar, and MA Islam. "Efficient Direct Organogenesis from Shoot Tips and Nodal Segments of Ash Gourd (Benincasa Hispida L.)." Journal of Life and Earth Science 8 (August 22, 2014): 17–20. http://dx.doi.org/10.3329/jles.v8i0.20135.

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The effect of external application of phytohormone on explants viz., shoot tips and nodal segments of ash gourd (Benincasa hispida L.) was tested on the frequency of shoot and root induction. Shoot tips and nodal segments were cultured on MS medium supplemented with different concentrations and combinations of cytokinins (BAP, Kinetin), auxin (IBA, NAA) and gibberellic acid (GA3) for multiple shoot formation and root induction. The highest number (up to 90%) of multiple shoot formation was obtained from the shoot tips in MS medium fortified with 1.5 mg/l BAP + 0.2 mg/l GA3, where average numbe

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15

Lualon, Wanwimon, Wanchai De-Eknamkul, Hiroyuki Tanaka, Yukihiro Shoyama, and Waraporn Putalun. "Artemisinin Production by Shoot Regeneration of Artemisia annua L. Using Thidiazuron." Zeitschrift für Naturforschung C 63, no. 1-2 (2008): 96–100. http://dx.doi.org/10.1515/znc-2008-1-218.

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An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The result shows that the stem segment cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l thidiazuron (TDZ) gave a perfect shoot formation (100%) and good shoot multiplication (57 shoots/expl

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16

Muthukumaran, P., N. Saraswathy, S. Abarna, et al. "Protocol for Induction of Multiple Shoot through Nodal Explants Culture of Bambusa bambos for Biomass Production." International Journal of Life-Sciences Scientific Research 4, no. 2 (2018): 1634–38. http://dx.doi.org/10.21276/ijlssr.2018.4.2.2.

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17

Tavallaie, F. Zaker, B. Ghareyazie, A. Bagheri, and K. K. Sharma. "Lentil Regeneration from Cotyledon Explant Bearing a Small Part of the Embryo Axis." Plant Tissue Culture and Biotechnology 21, no. 2 (2011): 169–80. http://dx.doi.org/10.3329/ptcb.v21i2.10240.

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The effect of external BAP on explants including leaflets, stems, cotyledons with and without embryo axis of lentil (Lens culinaris Medic.) were tested on the frequency of shoot induction. The effect of different levels and combinations of various cytokinins on multiple shoot induction and elongation of shoots were compared. The effect of explant age and pH of media were also tested on shoot induction from various explants. Finally, the effect of IBA, IAA, and NAA on root induction of elongated shoots were examined. Optimum conditions for root emergence and growth and the hardening of seedling

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18

List, SE, PH Brown, CS Low, and KB Walsh. "A micropropagation protocol for Melaleuca alternifolia (tea tree)." Australian Journal of Experimental Agriculture 36, no. 6 (1996): 755. http://dx.doi.org/10.1071/ea9960755.

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Micropropagation of Melaleuca alternifolia (tea tree) was achieved using stem segments from mature plants. A comparison of the source of explant material, and the form of cytolunin and mineral nitrogen on the induction of multiple shoots per node is reported. Further, the effect of shoot induction treatment, the age of plant used for explants and the value of the presence of charcoal, sucrose and light on subsequent root induction in plantlets is considered. A culture procedure involving nodes sourced from 1.5 to 3 cm from the shoot tip, cultured into a shoot induction medium of Murashige and

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19

Mantovani, Nilton César, Magali F. Grando, Aloisio Xavier, and Wagner C. Otoni. "In vitro shoot induction and multiplication from nodal segments of adult Ginkgo biloba plants." Horticultura Brasileira 31, no. 2 (2013): 184–89. http://dx.doi.org/10.1590/s0102-05362013000200003.

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The in vitro performance of herbaceous and woody nodal segments from adult plants and the effect of hydrolyzed casein (HC 500 mg L-1), kinetin (KIN; 6-furfurylaminopurine 0.46 and 4.65 µM) and activated charcoal (AC 1.5 g L-1) were evaluated upon new shoots induction and development, and to establish a system of in vitro propagation from adult plants of Ginkgo biloba. Woody nodal segments did not produce axillary shoots and presented 100% of bacterial and fungal contamination in culture. However, nodal segments from herbaceous shoots were successfully disinfected and displayed high in vitro mo

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20

Das, R., MF Hasan, MS Hossain, and M. Rahman. "Micropropagation of Centella asiatica L. An Important Medicinal Herb." Progressive Agriculture 19, no. 2 (2013): 51–56. http://dx.doi.org/10.3329/pa.v19i2.16928.

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High frequencies of multiple shoot regeneration were achieved from shoot tip explants cultured on MS media fortified with 4.0 mgl-1 BAP + 0.1 mgl-1 NAA. On an average 10.2?0.38 shoots per explant were obtained. The regenerated shoots were rooted in MS medium supplemented with 1.0 mgl-1 IBA. The in vitro grown plantlets were acclimatized and successfully transferred to natural condition with 80% survival. A reproducible protocol was established for in vitro propagation through multiple shoot induction of Centella asiatica L. an important medicinal herb having high medicinal value.DOI: http://dx

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21

Mohamed-Yasseen, Yasseen, Thomas L. Davenport, Walter E. Splittstoesser, and Robert M. Skirvin. "In vitro Propagation of Kurrat (Allium ampeloprasum, var. Kurrat) and Leek (Allium ampeloprasiun var. Porrum)." HortScience 28, no. 5 (1993): 508d—508. http://dx.doi.org/10.21273/hortsci.28.5.508d.

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Kurrat and leek are popular Middle-eastern and European vegetables in the family Liliaceae. Explants were obtained by longitudinally slicing, 3 to 4 times, seedling stem bases of leek and kurrat or by making multiple longitudinal slices across the central axis of mature kurrat stem bases which were previously cross cut 1 cm above the bases. Some explants were cultured directly for shoot-induction in Murashige and Skoog (MS) medium containing 4.4 μM benzyladenine. Others were allowed to form callus in MS medium containing 1.4 μM 2,4-D and 1.4 μM kinetin before transfer to the shoot-induction me

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22

Pachipala, Gayathri, Rajani Vemula, Vijaya Bhaskar Reddy Pichili, Krishna Jaswanth Boddupalli, and Kiranmai Chadipiralla. "Efficient In vitro Propagation and Optimized Multiple Shoot Induction of Caralluma bhupenderiana an Endangered Medicinal Plant." PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY 26, no. 3-4 (2025): 156–68. https://doi.org/10.56557/pcbmb/2025/v26i3-49252.

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Caralluma bhupenderiana is one of the medicinally important species within the Apocynaceae family. Recent In vitro studies revealed its medicinal properties particularly antioxidant and anti-inflammatory activities. C. bhupenderiana is considered an endemic, as it faces threats from habitat loss and overharvesting for medicinal and culinary uses. These challenges should be addressed using micropropagation techniques. Our study focuses on standardizing the protocol for inducing multiple shoots under In vitro conditions. Stem explants of C. bhupenderiana were treated with various concentrations

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23

Sekhukhune, Mapogo Kgetjepe, and Yvonne Mmatshelo Maila. "In Vitro Seed Germination, Seedling Development, Multiple Shoot Induction and Rooting of Actinidia chinensis." Plants 14, no. 6 (2025): 939. https://doi.org/10.3390/plants14060939.

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Worldwide, the yellow-fleshed kiwifruit (Actinidia chinensis) is an important crop that possesses great economic significance due to its nutritional, medicinal and ornamental values. The call for the expansion of the kiwifruit industry in South Africa, due to rising local and international market demand, resulted in the introduction of new plant species in sub-mountainous areas, where soil and climate conditions are more suitable for intensive kiwifruit production than in lowland areas. Consequently, a need to develop suitable commercial protocols for mass propagation of A. chinensis emerged.

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24

Ayyadurai., V., and K. Ramar. "IN VITRO DIRECT MULTIPLE SHOOT INDUCTION FROM LEAF EXPLANTS OF SOLANUM PUBESCENS WILD." International Journal of Research -GRANTHAALAYAH 4, no. 12SE (2016): 23–28. https://doi.org/10.5281/zenodo.230900.

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Efficientin Vitro direct multiple shoot regeneration from <em>Solanum pubescens</em> was achieved from leaf explants on MS medium Sublimated with B5 vitamins and different concentrations and different combinations of PGRs like BAP, NAA and GA3. The maximum numbers of multiple shoots were achieved from leaf explants on 3.0 mg/l BAP + 0.5mg/l NAA + 1.5mg/l GA3. The regenerated shoots were transferred in to half strength MS medium fortified with IBA for root induction. Rooted plantlets were successfully acclimatized. This new and transfer into the field Conditions. Standardized and reproducible p

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25

Nayak, Pranati, and Kalidass C. "In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants." Plant Tissue Culture and Biotechnology 26, no. 2 (2016): 255–66. http://dx.doi.org/10.3329/ptcb.v26i2.30575.

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Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA3. The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induct

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26

N. Kale, Anil, Pradip M. Adlinge, Vaibhav G. Waghmare, Vijay K. Raut, and Sachin L. Abhang. "Development of invitro Regeneration Protocol in Bitter Gourd (Momordica charantia)." International Journal of Current Microbiology and Applied Sciences 13, no. 2 (2024): 182–88. http://dx.doi.org/10.20546/ijcmas.2024.1302.025.

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In the present study an attempt was made for in vitro regeneration of bitter gourd cv. “BSS-1007”. In plant breeding tissue culture techniques play as an important role for rapid multiplication of particularly desirable genotype of F1 hybrids, gynoecious lines, triploid hybrid plants etc. where seed costs are high. Seeds of bitter gourd cv. BSS-1007 were surface sterilized with bavistin (0.1%) for 1 hr. followed by 70% ethanol (v/v) for 1 minutes and then 0.1% HgCl2 (w/v) solution for 5 minutes. After sterilization seeds were cultured on ½ MS medium for explants preparation. For direct multipl

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27

PALEE, Jiraporn. "In vitro Shoot Cultures of Tupistra albiflora K. Larsen." Walailak Journal of Science and Technology (WJST) 17, no. 5 (2018): 405–11. http://dx.doi.org/10.48048/wjst.2020.4182.

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To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the

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28

Sareeyuvaratch, W., S. Sengsai, K. Obsuwan, and C. Thepsithar. "Shoot initiation and multiple shoot induction of Sangmon ‘Nuan Rajinee’ bamboo (Dendrocalamus sericeus Munro)." Acta Horticulturae, no. 1298 (December 2020): 307–14. http://dx.doi.org/10.17660/actahortic.2020.1298.42.

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29

Jaheduzzaman, Dr, MA Habib, S. Akter, et al. "In- vitro regeneration of an important medicinal plant Centella asiatica L. Urban." Bangladesh Journal of Scientific and Industrial Research 47, no. 3 (2012): 269–72. http://dx.doi.org/10.3329/bjsir.v47i3.13058.

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An in vitro multiple shoot regeneration protocol has been developed for Centella asiatica L. Urban from the nodal and shoot tips explants using MS medium with different concentrations and combinations of growth regulators. Multiple shoot regeneration was observed from both the explants on MS containing either BAP alone or in combination with IAA, NAA and Kn. Of the two explants, nodal segment showed comparatively better response towards multiple shoot regeneration. Maximum multiple shoots were found on MS supplemented with 1.0 mg/L BAP and 0.4 mg/L NAA. For root induction, well-developed shoot

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30

Dona, Fauzia, Gustian Gustian, and Wulan Kumala Sari. "Pengaruh Konsentrasi Rootone F terhadap Induksi Akar dan Tunas Stek Pucuk Kopi Arabika (Coffea arabica)." Jagur Jurnal Agroteknologi 5, no. 1 (2023): 1. http://dx.doi.org/10.25077/jagur.5.1.1-6.2023.

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Coffee is a plantation commodity which important to increase its production, such as by vegetative propagation i.e., shoot cuttings that stimulated by Plant Growth Regulators (PGR). The research was on the effect of Rootone F concentrations on roots and shoots induction of Arabica coffee (Coffea arabica) shoot cuttings, it was carried out at the smallholder coffee plantation Solok Radjo in Aie Dingin Village, Lembah Gumanti District, Solok Regency on April until August 2022. The objectives of this study were to determine the growth response of Arabica coffee shoot cuttings on the induction of

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31

Shyamkumar, B., C. Anjaneyulu, and C. C. Giri. "Multiple Shoot Induction from Cotyledonary Node Explants of Terminalia chebula." Biologia plantarum 46, no. 4 (2003): 585–88. http://dx.doi.org/10.1023/b:biop.0000041066.78766.34.

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Kumar, Suresh, and Amaresh Chandra. "Direct Plant Regeneration via Multiple Shoot Induction in Stylosanthes seabrana." CYTOLOGIA 74, no. 4 (2009): 391–99. http://dx.doi.org/10.1508/cytologia.74.391.

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Khan, S., S. Akter, A. Habib, et al. "Establishment of in vitro regeneration protocol for Adhatoda vasica Nees." Bangladesh Journal of Scientific and Industrial Research 51, no. 1 (2016): 75–80. http://dx.doi.org/10.3329/bjsir.v51i1.27077.

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An in vitro regeneration protocol of Adhatoda vasica has been developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA3 and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were obs

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Yesmin, Sabina, Sanjida Rahman Mollika, Md Nazrul Islam, and Shamima Nasrin. "In vitro Regeneration of two BINA Tomato (Lycopersicon esculentum Mill.) Varieties of Bangladesh." Plant Tissue Culture and Biotechnology 32, no. 1 (2022): 43–51. http://dx.doi.org/10.3329/ptcb.v32i1.60471.

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Cotyledonary leaf from 12 days old aseptically grown seedlings were used as explant. Cotyledonary leaf explants of two BINA varieties of tomato were cultured on MS medium containing different concentrations and combinations of BAP, Kn and IAA for multiple shoot formation via organogenesis. 100% shoot induction was observed when the explants were cultured on MS medium supplemented with BAP, Kn and IAA. Earliest shoot induction was observed within four weeks. The MS media supplemented with 1.5 mg/l BAP + 0.5 mg/l Kn + 0.2 mg/l IAA found to be the best medium with regards to number of days requir

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35

Kumari. R, Usha, S. M. Lakshmi, and G. Thamodharan. "Effect of Growth hormones for direct organogenesis in Bael (Aegle marmelos (L.) corr)." Journal of Applied and Natural Science 7, no. 1 (2015): 98–101. http://dx.doi.org/10.31018/jans.v7i1.570.

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Aegle marmelos (L.) corr. commonly known as ‘Bael’ tree is included in the red- listed medicinal tree in South India. Riped and unripe fruits, root, bark and leaves of the tree are used in ayurvedic preparation for various ailments. This study was conducted to standardize the media for multiple shoot induction from different explants under in vitro condition. Among the various explants viz., meristematic tip, nodal bud, tender leaf, auxillary bud and cotyledon tried in different media, auxiliary bud had shown good response for shoot induction in 36 to 39 days. Among the various treatments viz.

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Gosukonda, Ramana M., C. S. Prakash, and Ananta Porobo Dessai. "Shoot Regeneration in Vitro from Diverse Genotypes of Sweetpotato and Multiple Shoot Production per Explant." HortScience 30, no. 5 (1995): 1074–77. http://dx.doi.org/10.21273/hortsci.30.5.1074.

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Studies were conducted to improve adventitious shoot regeneration in sweetpotato [Ipomoea batatas (L.) Lam.], specifically to extend the protocol to many genotypes and to elicit production of multiple shoots per explant. The use of a two-stage procedure where excised petioles were incubated on Murashige and Skoog (MS) (1962) medium with 2,4-D (0.2 mg·liter–1) for 3 days and transferred to a second medium containing MS salts with thidiazuron and 2iP (0.05 mg·liter–1 each) resulted in shoot regeneration from eight of 13 genotypes tested, including elite sweetpotato cultivars such as `Jewel' and

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37

Amirova, Aigul, Symbat Dossymbetova, Yeldana Rysbayeva, Bakdaulet Usenbekov, Arman Tolegen, and Alibek Ydyrys. "Multiple Plant Regeneration from Embryogenic Calli of Paulownia tomentosa (Thunb.) Steud." Plants 11, no. 8 (2022): 1020. http://dx.doi.org/10.3390/plants11081020.

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The aim of this paper was to study the effect of plant growth regulators on callus induction and in vitro morphogenesis using various explants of Paulownia tomentosa to develop an efficient plant regeneration protocol. Different plant organ sections (leaves, apical shoot tips, petals, nodes, and internodes) were cultured as explants to identify the best in vitro explants responsive to callus induction and plant regeneration. Explants were cultivated on MS media supplemented with different concentrations of plant growth regulators (TDZ (Thidiazuron), BAP (6-Benzylaminopurine), kinetin, and NAA

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V, Ayyadurai, and Ramar K. "IN VITRO DIRECT MULTIPLE SHOOT INDUCTION FROM LEAF EXPLANTS OF SOLANUM PUBESCENS WILLD." International Journal of Research -GRANTHAALAYAH 4, no. 12SE (2016): 23–28. http://dx.doi.org/10.29121/granthaalayah.v4.i12se.2016.2475.

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Efficientin Vitro direct multiple shoot regeneration from Solanum pubescens was achieved from leaf explants on MS medium Sublimated with B5 vitamins and different concentrations and different combinations of PGRs like BAP, NAA and GA3. The maximum numbers of multiple shoots were achieved from leaf explants on 3.0 mg/l BAP + 1.0mg/l GA3. The regenerated shoots were transferred in to half strength MS medium fortified with IBA for root induction. Rooted plantlets were successfully acclimatized. This new and transfer into the field Conditions. Standardized and reproducible protocol useful the mass

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Islam, Mohammed Rafiqul, Mustari Taslim Jahan, and Md Aminul Islam. "Establishment of a Suitable Protocol for Clonal Propagation of Stevia (Stevia rebaudiana) and Creating its Advanced Lines using Gamma-Ray." Journal of Science and Technology Research 6, no. 1 (2024): 99–107. https://doi.org/10.3329/jscitr.v6i1.77381.

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A suitable protocol was developed for in vitro clonal propagation of Stevia rebaudiana Bertoni from shoots tip and nodal explant. Experiments were conducted to standardize the culture media composition with plant hormone for multiple shoot proliferation and rooting for obtaining plantlets with uniform characteristics like the mother plant in terms of growth and habits. The best response towards multiple shoot regeneration was observed from nodal explants on Murashige and Skoog (MS) basal media fortified with 3.0 mg/L Benzyl amino purine (BAP). In this combination, an average of 14 shoots were

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Zou, Li-Juan, Jin-Yao Hu, Ming-Hua Luo, and Qing-Gui Wu. "In vitro propagation of the Chinese traditional and medicinal plant Heracleum scabridum Franch." Bangladesh Journal of Botany 48, no. 3 (2019): 575–81. http://dx.doi.org/10.3329/bjb.v48i3.47933.

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Three explants such as stem tips, leaves and petioles of Heracleum scabridum were compared for their shoot development/differentiation ability by using different plant growth regulators (PGRs). The most effective PGRs combination for callus induction and organogenesis was determined. TDZ, Kn, Zn and IAA were used to induce multiple shoots. For indirect organogenesis (from the calli), the best response (27.25 ± 4.19 shoot per stem tips) and (18.23 ± 2.12 per leaves) were obtained in Murashige and Skoog (MS) medium fortified with 0.5 mg/l IAA and 3.0 mg/l Zn with 100, 97.3% induction rate, respe

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Roy, PK. "In vitro plant regeneration of Paulownia tomentosa (Thunb.) Steud. from shoot tip and leaf segment." Bangladesh Journal of Botany 44, no. 3 (2018): 459–63. http://dx.doi.org/10.3329/bjb.v44i3.38555.

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An efficient protocol was developed for in vitro mass propagation of Paulownia tomentosa (Thunb.) Steud. using shoot tip and leaf segment explants from field grown plant. Different concentrations and combinations of BAP, Kn, zeatin and NAA were used for multiple shoot regeneration. Among two types of explants, leaf segment produced the highest number of shoots per explant (12 ± 0.4) when they were cultured on MS supplemented with 3.0 mg/l Kn and 0.5 mg/l NAA. Addition of 10% CW to above mentioned medium increased the number of shoots (18) per culture. Shoot tip explants also produced multiple

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Opabode, Jelili Titilola, Olufemi Victor Ajibola та Oluyemisi Amos Akinyemiju. "Shoot Induction from Axillary Bud of β-Carotene Enriched Manihot esculenta Crantz and Molecular Stability of Regenerants". Agricultura Tropica et Subtropica 48, № 3-4 (2015): 53–58. http://dx.doi.org/10.1515/ats-2015-0008.

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Abstract Inadequacy of planting materials is limiting the distribution of three high-β-carotene (pro-vitamin A) cassava varieties released in Nigeria to increase public vitamin A intake. However, studies have established the suitability of in vitro shoot culture for mass propagation of elite crop varieties for quick distribution to farmers. We investigated multiple shoot induction from axillary bud of three β-carotene enriched cassava varieties using BAP and assessed the genetic stability of the regenerated plants to establish mass propagation system. Multiple shoots were induced from BAP-enla

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Chamandoosti, Firoozeh. "Citrus TISSUE CULTURE WITH TWO DIFFERENT APPROACHES." International Journal of Biosciences and Biotechnology 8, no. 1 (2020): 19. http://dx.doi.org/10.24843/ijbb.2020.v08.i01.p03.

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Citrus is an important genius for economy and human health but a susceptible genius against biotic and abiotic stresses, so it needs to improved programs. Different basal media (MS, ½MA, ?MS and DKW) and different kind and concentrations of plant growth regulators i.e. BA, KIN, 2ip, ZE and TDZ (0 – 2 mg/l) and NAA, IAA and IBA (0 – 2 mg/l) added with 30 g/l sucrose, 3 g/l active charcoal and 7.5 g/l bacteriological agar] were used for organogenesis include shooting and rooting, also callusing from nodal explant of Citrus latifolia. MS medium supplemented with 1 mg/l BA, and 0.01 mg/l NAA is th

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Warhade, Mrunal I., and Rupesh S. Badere. "Studies on micropropagation of Rosa setigera michx. through multiple shoot induction." Journal of Indian Botanical Society 98, no. 3and4 (2019): 125. http://dx.doi.org/10.5958/2455-7218.2019.00015.9.

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Mooney, Pauline A., and J. van Staden. "In vitro plantlet formation and multiple shoot induction in Sargassum heterophyllum." South African Journal of Botany 51, no. 1 (1985): 41–44. http://dx.doi.org/10.1016/s0254-6299(16)31700-8.

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Hiregoudar, L. V., H. N. Murthy, B. P. Hema, E. J. Hahn, and K. Y. Paek. "Multiple shoot induction and plant regeneration of Feronia limonia (L.) Swingle." Scientia Horticulturae 98, no. 4 (2003): 357–64. http://dx.doi.org/10.1016/s0304-4238(03)00018-9.

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Das, D. K., N. Shiva Prakash, and N. Bhalla-Sarin. "Multiple shoot induction and plant regeneration in litchi ( Litchi chinensis Sonn.)." Plant Cell Reports 18, no. 7-8 (1999): 691–95. http://dx.doi.org/10.1007/s002990050644.

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Umami, N., E. R. V. Rahayu, B. Suhartanto, and N. Suseno. "The effect of 2,4-dichlorophenoxyacetic acid, benzyl amino purin and cupric sulphate on in vitro propagation system from shoot apices of shoot tiller of hybrid Napier grass (Pennisetum purpureum Schum)." IOP Conference Series: Earth and Environmental Science 951, no. 1 (2022): 012088. http://dx.doi.org/10.1088/1755-1315/951/1/012088.

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Abstract An efficient micropropagation method of hybrid Napier grass (Pennisetum purpureumn Schum) for in vitro plant production and material breeding was established from multiple-shoot clumps (MCS) regeneration system. This system was important for forage breeding system. Shoot apices from shoot-tillers produced MSC on Murashige-Skoog (MS) induction medium containing several combinations of BAP and 2,4-D in induction stage. The addition of 5 μM (v/v) and 50 μM (v/v) CuSO4 were added in best medium for inoculation to proliferate the clump in proliferation/multiplication stage. Plant regenerat

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Waneck, Don, H. Mathews, J. Stamp, and R. Bestwick. "802 PB 106 PLANT REGENERATION THROUGH SOMATIC EMBRYOGENESIS AND ORGANOGENESIS IN GRAPE ROOT STOCK CULTIVAR AXR#1." HortScience 29, no. 5 (1994): 548b—548. http://dx.doi.org/10.21273/hortsci.29.5.548b.

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Zygotic embryo explants of grape cultivar AXR#1 were isolated from maw-e seeds and cultured on medium supplemented with naphthoxy acetic acid beta-(NOA) and benzylaminopurine (BA). Embryo explants dedifferentiated to form embryogenic callus. Globular stage embryos were visible in 9-10 months. On transfer 10 a growth regulator free medium supplemented with charcoal these globular embryos underwent further stages of embryo development. In a period of 30-40 days embryogenic tissues turned into clumps of somatic embryos displaying different stages of development Cotyledonary stage embryos were sep

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Abraham, Jyothi, and T. Dennis Thomas. "An efficient shoot regeneration system for medicinally important Elephantopus scaber Linn." Crop Breeding and Applied Biotechnology 15, no. 2 (2015): 94–99. http://dx.doi.org/10.1590/1984-70332015v15n2a17.

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An efficient protocol for the rapid micropropagation of medicinally important Elephantopus scaber has been standardized using cotyledonary node explants. Direct multiple shoot induction was observed when the cotyledonary node explants at various age groups were cultured on MS medium supplemented with various plant growth regulators. The highest shoot induction was obtained when the cotyledonary node explants from 20-day-old seedlings were cultured on MS medium supplemented with 1.5 mg L-1 TDZ and 0.5 mg L-1 NAA. On this medium, 98% of the cultures responded, with an average number of 33.7 shoo

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