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1

Aloo, Simon Okomo, Fred Kwame Ofosu, Sheila M. Kilonzi, Umair Shabbir, and Deog Hwan Oh. "Edible Plant Sprouts: Health Benefits, Trends, and Opportunities for Novel Exploration." Nutrients 13, no. 8 (2021): 2882. http://dx.doi.org/10.3390/nu13082882.

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The consumption of plant sprouts as part of human day-to-day diets is gradually increasing, and their health benefit is attracting interest across multiple disciplines. The purpose of this review was to (a) critically evaluate the phytochemicals in selected sprouts (alfalfa, buckwheat, broccoli, and red cabbage), (b) describe the health benefits of sprouts, (c) assess the recent advances in sprout production, (d) rigorously evaluate their safety, and (e) suggest directions that merit special consideration for further novel research on sprouts. Young shoots are characterized by high levels of h
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2

Dębski, Henryk, Wiesław Wiczkowski, and Marcin Horbowicz. "Effect of Elicitation with Iron Chelate and Sodium Metasilicate on Phenolic Compounds in Legume Sprouts." Molecules 26, no. 5 (2021): 1345. http://dx.doi.org/10.3390/molecules26051345.

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Seven-day-old sprouts of fenugreek (Trigonella foenum-graecum L.), lentil (Lens culinaris L.), and alfalfa (Medicagosativa L.) were studied. The legume seeds and then sprouts were soaked each day for 30 min during 6 days with water (control) or mixture of Fe-EDTA and sodium silicate (Optysil), or sodium silicate (Na-Sil) alone. Germination and sprout growing was carried out at temperature 20 ± 2 °C in 16/8 h (day/night) conditions. Phenolic compounds (free, ester, and glycosides) content were determined by HPLC-ESI-MS/MS using a multiple reaction monitoring of selected ions. Flavonoids and phe
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3

Burnett, Christopher D., and David Gilluly. "Pollarding for Multiple Benefits." Northern Journal of Applied Forestry 5, no. 2 (1988): 148–52. http://dx.doi.org/10.1093/njaf/5.2.148.

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Abstract Pollarding is a traditional method of producing fuelwood in agroforestry systems where livestock browsing restricts tree regeneration by seedlings or coppice sprouts. As in coppicing, pollarding produces successive crops of wood from the vegetative sprouts of repeatedly harvested trees, but in pollarding the dominant sprouts are formed near the top of permanent trunks above the reach of livestock. The unique structure of pollards also makes them suitable for use as short-rotation fuelwood trees where fire restricts tree regeneration at ground level. In addition to fuelwood, pollards p
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4

HORA, R., M. KUMAR, L. GARCIA, B. SCHUMACHER, J. ODUMERU, and K. WARRINER. "Spatial Distribution of Salmonella, Escherichia coli O157:H7, and Other Bacterial Populations in Commercial and Laboratory-Scale Sprouting Mung Bean Beds." Journal of Food Protection 68, no. 12 (2005): 2510–18. http://dx.doi.org/10.4315/0362-028x-68.12.2510.

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The reliability of testing spent irrigation water to assess the microbiological status of sprouting mung bean beds has been investigated. In commercial trials, the distribution of opportunistic contaminants within 32 bean sprout beds (25 kg of mung beans per bin) was assessed 48 h after germination. The prevalence of generic Escherichia coli, thermotolerant coliforms, and Aeromonas in sprouts (n = 288) was 5, 11, and 39%, respectively, and 57, 70, and 79% in the corresponding spent irrigation water samples (n = 96). Contamination was heterogeneously distributed within the seedbed. In laborator
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5

Moreno, Diego A., Santiago Pérez-Balibrea, and Cristina García-Viguera. "Phytochemical Quality and Bioactivity of Edible Sprouts." Natural Product Communications 1, no. 11 (2006): 1934578X0600101. http://dx.doi.org/10.1177/1934578x0600101120.

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Edible sprouts are phytonutrient-rich plant foods, good source of flavonoids, other polyphenols, glucosinolates, isothiocyanates, proteins, minerals and vitamins. The increasing consumption of sprouts requires optimisation of their quality, palatability and bioactivity. Multiple genetic and environmental factors (growth conditions, stress, elicitors) affect the production and accumulation of phytochemicals in these foods, offering the basis for further research on the improvement of the nutritional and health-relevant functional value of edible sprouts. In the present review, we focus on the p
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6

Sakamoto, Masaru, and Takahiro Suzuki. "Methyl Jasmonate and Salinity Increase Anthocyanin Accumulation in Radish Sprouts." Horticulturae 5, no. 3 (2019): 62. http://dx.doi.org/10.3390/horticulturae5030062.

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Plant secondary metabolites with antioxidant properties, such as anthocyanins, are considered to have an important commercial value for some crops. Although anthocyanin concentration increases in response to various stimuli in plants, the mechanism of anthocyanin accumulation under multiple stimuli is not yet well understood. Here, we examined the effects of methyl jasmonate (MJ) and salinity on anthocyanin accumulation in radish (Raphanus sativus) sprouts. MJ treatments induced anthocyanin accumulation, which was enhanced by simultaneous treatment with salinity (200 mM NaCl), accompanied by g
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7

Leyva-López, Norma Elena, Juan Carlos Ochoa-Sánchez, Diana Sara Leal-Klevezas, and Juan Pablo Martínez-Soriano. "Multiple phytoplasmas associated with potato diseases in Mexico." Canadian Journal of Microbiology 48, no. 12 (2002): 1062–68. http://dx.doi.org/10.1139/w02-109.

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In recent years, the potato crop in Mexico has been notably affected by diseases recognized as potato purple top (PPT) in foliage and potato hair sprouts (PHS) in germinating tubers. In both cases, these syndromes reduce production by affecting viability of the tubers used as seeds. There is evidence indicating that phytoplasmas are associated with these syndromes. This study presents data on the molecular detection, characterization, and ecology of the pathogens related to PPT and PHS. Restriction fragment length polymorphism (RFLP) and sequence analysis indicated that PPT phytoplasma belongs
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8

Klyachenko, O., and O. Nekrut. "Morphogenesis of Miscanthus × Giganteus in vitro." Agricultural Science and Practice 5, no. 2 (2018): 13–17. http://dx.doi.org/10.15407/agrisp5.02.013.

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Aim. To obtain planting material of Miscanthus × giganteus in a large amount in order to study the peculiari- ties of morphogenesis of isolated meristem culture. Methods. The explants were sterilized using 70 % etha- nol and 0.1 % mercury bichloride, after that, the methods of plant cultivation and reproduction in vitro and statistical processing of the obtained data was performed. Results. The study presents the results of obtaining the regenerate plants from dormant buds of 3-year-old rhizomes of Miscanthus × giganteus. The Murashige and Skoog medium supplemented with 6-benzylaminopurine (BA
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9

Matthysse, Ann G., Rajendar Deora, Meenu Mishra та Alfredo G. Torres. "Polysaccharides Cellulose, Poly-β-1,6-N-Acetyl-d-Glucosamine, and Colanic Acid Are Required for Optimal Binding of Escherichia coli O157:H7 Strains to Alfalfa Sprouts and K-12 Strains to Plastic but Not for Binding to Epithelial Cells". Applied and Environmental Microbiology 74, № 8 (2008): 2384–90. http://dx.doi.org/10.1128/aem.01854-07.

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ABSTRACT When Escherichia coli O157:H7 bacteria are added to alfalfa sprouts growing in water, the bacteria bind tightly to the sprouts. In contrast, laboratory K-12 strains of E. coli do not bind to sprouts under similar conditions. The roles of E. coli O157:H7 lipopolysaccharide (LPS), capsular polysaccharide, and exopolysaccharides in binding to sprouts were examined. An LPS mutant had no effect on the binding of the pathogenic strain. Cellulose synthase mutants showed a significant reduction in binding; colanic acid mutants were more severely reduced, and binding by poly-β-1,6-N-acetylgluc
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10

Subedi, Lalita, KyoHee Cho, Yong Un Park, Hyuk Joon Choi, and Sun Yeou Kim. "Sulforaphane-Enriched Broccoli Sprouts Pretreated by Pulsed Electric Fields Reduces Neuroinflammation and Ameliorates Scopolamine-Induced Amnesia in Mouse Brain through Its Antioxidant Ability via Nrf2-HO-1 Activation." Oxidative Medicine and Cellular Longevity 2019 (March 27, 2019): 1–19. http://dx.doi.org/10.1155/2019/3549274.

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Activated microglia-mediated neuroinflammation plays a key pathogenic role in neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, and ischemia. Sulforaphane is an active compound produced after conversion of glucoraphanin by the myrosinase enzyme in broccoli (Brassica oleracea var) sprouts. Dietary broccoli extract as well as sulforaphane has previously known to mitigate inflammatory conditions in aged models involving microglial activation. Here, we produced sulforaphane-enriched broccoli sprouts through the pretreatment of pulsed electric fields
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11

Sakai, Kenji, Chiho Ishida, Yuko Kato-Motozaki, Atsuro Tagami, Kiyonobu Komai, and Masahito Yamada. "Somatic sprouts of the Purkinje cells in a patient with multiple system atrophy." Neuropathology 38, no. 4 (2018): 407–10. http://dx.doi.org/10.1111/neup.12464.

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12

Schepici, Giovanni, Placido Bramanti, and Emanuela Mazzon. "Efficacy of Sulforaphane in Neurodegenerative Diseases." International Journal of Molecular Sciences 21, no. 22 (2020): 8637. http://dx.doi.org/10.3390/ijms21228637.

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Sulforaphane (SFN) is a phytocompound belonging to the isothiocyanate family. Although it was also found in seeds and mature plants, SFN is mainly present in sprouts of many cruciferous vegetables, including cabbage, broccoli, cauliflower, and Brussels sprouts. SFN is produced by the conversion of glucoraphanin through the enzyme myrosinase, which leads to the formation of this isothiocyanate. SFN is especially characterized by antioxidant, anti-inflammatory, and anti-apoptotic properties, and for this reason, it aroused the interest of researchers. The aim of this review is to summarize the e
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13

Davis, Jaimie, Katie Nikah, Fiona Asigbee, et al. "TX Sprouts: A School-Based Cluster Randomized Gardening, Nutrition, and Cooking Intervention: Effects on Obesity, Blood Pressure and Diet." Current Developments in Nutrition 4, Supplement_2 (2020): 1296. http://dx.doi.org/10.1093/cdn/nzaa059_013.

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Abstract Objectives To assess the effects of a one-year school-based gardening, nutrition, and cooking cluster randomized controlled trial, called TX Sprouts, on dietary intake, obesity markers, and blood pressure. Methods Sixteen schools were randomly assigned to either the TX Sprouts intervention (n = 8 school) or to delayed intervention (n = 8 schools). The intervention arm received: formation/training of Garden Leadership Committees; a 0.25-acre outdoor teaching garden; 18 student lessons including gardening, nutrition, and cooking activities, taught weekly during school hours; and nine pa
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14

Grant, WJR, and BR Loveys. "Controlling rootstock sprouts of Agonis flexuosa, (Willd.) Sweet at ambient and elevated CO2 by multiple applications of low concentration NAA." Australian Journal of Experimental Agriculture 36, no. 5 (1996): 619. http://dx.doi.org/10.1071/ea9960619.

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A method for grafting variegated scion material to green leafed Agonis flexuosa (Willd.) Sweet stock was developed to overcome the difficulty of striking cuttings. However sprouting of both seedling and cutting-grown A. flexuosa rootstocks was a significant problem. Microwedge grafting of actively growing leafy scions and stocks in fog at 32/22�C gave 90-100% success and scion bud activity was stimulated within 2 weeks. Weekly or fortnightly spray applications of 100 mg NAA/L (napthaleneacetic acid), starting at the time of grafting, gave effective sprout control whereas a single pre-graft spr
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15

Galieni, Angelica, Beatrice Falcinelli, Fabio Stagnari, Alessandro Datti, and Paolo Benincasa. "Sprouts and Microgreens: Trends, Opportunities, and Horizons for Novel Research." Agronomy 10, no. 9 (2020): 1424. http://dx.doi.org/10.3390/agronomy10091424.

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Sprouts and microgreens have attracted tremendous interest across multiple disciplines in recent years. Here, we critically review the most recent advances to underscore research prospects and niches, and related challenges, not yet addressed or fully pursued. In particular, we report a number of themes that merit special attention as a result of their relevance to plant science, nutrition, health, and zootechnics: (1) species not yet or inadequately investigated, such as wild plants, and fruit tree strains; (2) abiotic and biotic factors, and biostimulants, for elicitation strategies and meta
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16

Laksono, Rommy Andhika, Fawzy Muhamad Bayfurqon, and Miftakhul Bakhrir R.K. "Uji Efektivitas Berbagai Konsentrasi Jenis Nutrisi Alternatif Terhadap Produksi Jamur Tiram Putih (Pleurotus ostreatus) di Kabupten Karawang." Paspalum: Jurnal Ilmiah Pertanian 6, no. 1 (2018): 32. http://dx.doi.org/10.35138/paspalum.v6i1.75.

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In the development of modern agriculture based on location specification agriculture, to increase the production of white oyster mushrooms, requires able to utilize alternative natural resources that are easy to obtain and efficient.The research was conducted with the aim to study and get the concentration of alternative nutrient types that can provide the highest production of white oyster mushroom in Karawang regency. Based on the purpose, this research is verifikatif.The method used is experimental method and experiment design used is single factor randomized block design (RAK) consisting o
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17

Nurjanati, Milla, and Hery Winarsi. "EFEK LAMA PERKECAMBAHAN TERHADAP SIFAT SENSORI DAN KADAR PROTEIN TERLARUT SUSU KECAMBAH KACANG MERAH (Sukarah) UNTUK REMAJA OBESITAS." Jurnal Gizi dan Pangan Soedirman 2, no. 2 (2019): 27. http://dx.doi.org/10.20884/1.jgps.2018.2.2.1352.

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This study aims to determine the effect of germination on the nature of sensory and dissolved protein content of red bean sprouts milk. This non-factorial experimental study used a randomized block design. The factors studied were germination time: 0 hours, 8 hours, 16 hours, and 24 hours. Parameters tested were soluble protein content and sensory properties including color, flavor, taste, and viscosity. Sensory test using hedonic test and hedonic quality with 50 panelist. The results were analyzed using Friedman test followed by Multiple Comparison Test at 5% level, if there is significantly
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Mooy, Herlistin, Anne Nuraini, and Sumadi Sumadi. "Respons perkecambahan benih jagung manis terhadap konsentrasi dan lama perendaman giberelin pada suhu lingkungan yang berbeda." Kultivasi 20, no. 1 (2021): 53. http://dx.doi.org/10.24198/kultivasi.v20i1.25921.

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Abstrak. Penanaman jagung manis pada daerah dengan suhu rendah dapat menyebabkan cekaman pada kecambah, sehingga performa kecambah perlu ditingkatkan. Penelitian ini bertujuan untuk menganalisis pengaruh interaksi antara konsentrasi giberelin (GA3) dan lama waktu perendaman benih pada suhu lingkungan tertentu terhadap pertumbuhan kecambah jagung manis. Penelitian ini dilaksanakan pada Laboratorium Teknologi Benih Fakultas Pertanian, Universitas Padjadjaran Bandung pada bulan Juni 2019. Rancangan percobaan menggunakan Rancangan Acak Kelompok pola faktorial dengan dua faktor dan tiga ulangan. Fa
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19

Su, Shih-Chi, E. Adriana Mendoza, Hyeong-il Kwak, and Kayla J. Bayless. "Molecular profile of endothelial invasion of three-dimensional collagen matrices: insights into angiogenic sprout induction in wound healing." American Journal of Physiology-Cell Physiology 295, no. 5 (2008): C1215—C1229. http://dx.doi.org/10.1152/ajpcell.00336.2008.

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Sprouting angiogenesis is a multistep process consisting of basement membrane degradation, endothelial cell (EC) activation, proliferation, invasion, lumen formation, and sprout stabilization. Such complexity is consistent with a requirement for orchestration of individual gene expression alongside multiple signaling pathways. To better understand the mechanisms that direct the transformation of adherent ECs on the surface of collagen matrices to develop multicellular invading sprouts, we analyzed differential gene expression with time using a defined in vitro model of EC invasion driven by th
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20

Dewi, Kumala, and Parmi _. "THE EFFECT OF GLUCOSE AND LIGHT QUALITY ON SEED GERMINATION, VITAMIN E AND CHLOROPHYLL CONTENT OF GREEN BEAN SPROUT (Vigna radiata (L.) Wilcz.)." KnE Life Sciences 2, no. 1 (2015): 317. http://dx.doi.org/10.18502/kls.v2i1.165.

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<p>Environmental factors such as the existence of exogenous sugar and light quality during seed germination may affect the growth and quality of seedlings. This experiment was aimed to evaluate the effect of glucose and light quality on seed germination percentage, vitamine E and chlorophyll content in green bean sprout (Vigna radiata (L.) Wilcz). The experiment design used was 5x5 factorial. The main factor was glucose concentration which consisted of 5 levels (0%, 0.5%, 1%, 2.5% or 5 %). The second factor was variation of light quality which consists of 3 different light spectrums name
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Lobur, David, Diwakar Davar, Shivendra Singh, et al. "Pilot evaluation of sulforaphane in melanoma patients with multiple atypical nevi: Tissue STAT1 and STAT3 as risk markers." Journal of Clinical Oncology 30, no. 15_suppl (2012): TPS8606. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.tps8606.

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TPS8606 Background: Increasing incidence and the poor prognosis of advanced melanoma argue for prevention efforts. Primary prevention and ultraviolet radiation (UVR) reduction have failed to gain traction in the population, making chemoprevention increasingly attractive. Sulforaphanes (SFN) are bioactive cruciferous compounds rich in the Brassica family, especially broccoli sprouts. Topical broccoli sprout extracts (BSE) decrease UVR erythema response in human skin, and may protect against UVR DNA damage. We have shown SFN inhibition of STAT3, which is constitutively activated in melanoma. We
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Vu Ngoc, Tu, Chuc Nguyen Sy, Chi Le Dinh, Anh Huong Nguyen Thi, and Son Tran Cao. "Development and validation of LC-MS/MS method for simultaneous determination of 6-benzylaminopurine and forchlorfenuron in bean sprout." Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 3, no. 3 (2020): 154–64. http://dx.doi.org/10.47866/2615-9252/vjfc.1725.

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The overuse of plant growth promoters in agriculture, especially those derived from a synthetic cytokinin group, is increasing rapidly. In this study, the method for determining two plant growth hormones of the cytokinin group, including 6-benzylaminopurine and forchlorfenuron in bean sprouts, was developed based on the QuEChERS extraction technique in combination with LC-MS/MS detection and quantification. Chromatographic conditions include C18 reversed phase column, a gradient mobile phase system with two channels of H2O and MeOH, with the addition of 0.1% formic acid and 10 mM amonium forma
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23

Park, Hyerin, Eunok Lee, Yunsoo Kim, Hye Yoon Jung, Kwang-Min Kim, and Oran Kwon. "Metabolic Profiling Analysis Reveals the Potential Contribution of Barley Sprouts against Oxidative Stress and Related Liver Cell Damage in Habitual Alcohol Drinkers." Antioxidants 10, no. 3 (2021): 459. http://dx.doi.org/10.3390/antiox10030459.

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Chronic excessive alcohol consumption is associated with multiple liver defects, such as steatosis and cirrhosis, mainly attributable to excessive reactive oxygen species (ROS) production. Barley sprouts (Hordeum vulgare L.) contain high levels of polyphenols that may serve as potential antioxidants. This study aimed to investigate whether barley sprouts extract powder (BSE) relieves alcohol-induced oxidative stress and related hepatic damages in habitual alcohol drinkers with fatty liver. In a 12-week randomized controlled trial with two arms (placebo or 480 mg/day BSE; n = 76), we measured c
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Murashima, Megumi, Shaw Watanabe, Xing-Gang Zhuo, Mariko Uehara, and Atsushi Kurashige. "Phase 1 study of multiple biomarkers for metabolism and oxidative stress after one-week intake of broccoli sprouts." BioFactors 22, no. 1-4 (2004): 271–75. http://dx.doi.org/10.1002/biof.5520220154.

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Degani, Ofir, and Ben Kalman. "Assessment of Commercial Fungicides against Onion (Allium cepa) Basal Rot Disease Caused by Fusarium oxysporum f. sp. cepae and Fusarium acutatum." Journal of Fungi 7, no. 3 (2021): 235. http://dx.doi.org/10.3390/jof7030235.

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The onion basal rot disease is a worldwide threat caused by species of the genus Fusarium. Today, Israel’s control of this disease is limited to a four-year growth cycle and Metam sodium soil disinfection. Here, commercial chemical fungicides were evaluated as control treatments against two of the primary pathogens involved, F. oxysporum f. sp. cepae and F. Acutatum. Out of 10 fungicides tested on culture plates, 3, Prochloraz, Azoxystrobin + Tebuconazole, and Fludioxonil + Sedaxen, had strong inhibitory effects on mycelial growth and were selected and tested in seeds in vitro. The preparation
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Zhang, Hua, Xingqiang Wu, Yanan Yuan, Dandan Han, Fengxia Qiao, and Hongyuan Yan. "An ionic liquid functionalized graphene adsorbent with multiple adsorption mechanisms for pipette-tip solid-phase extraction of auxins in soybean sprouts." Food Chemistry 265 (November 2018): 290–97. http://dx.doi.org/10.1016/j.foodchem.2018.05.090.

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27

ERICKSON, MARILYN C., and MICHAEL P. DOYLE. "Food as a Vehicle for Transmission of Shiga Toxin–Producing Escherichia coli." Journal of Food Protection 70, no. 10 (2007): 2426–49. http://dx.doi.org/10.4315/0362-028x-70.10.2426.

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Contaminated food continues to be the principal vehicle for transmission of Escherichia coli O157:H7 and other Shiga toxin–producing E. coli (STEC) to humans. A large number of foods, including those associated with outbreaks (alfalfa sprouts, fresh produce, beef, and unpasteurized juices), have been the focus of intensive research studies in the past few years (2003 to 2006) to assess the prevalence and identify effective intervention and inactivation treatments for these pathogens. Recent analyses of retail foods in the United States revealed E. coli O157:H7 was present in 1.5% of alfalfa sp
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GARNER, DANISHA, and SOPHIA KATHARIOU. "Fresh Produce–Associated Listeriosis Outbreaks, Sources of Concern, Teachable Moments, and Insights." Journal of Food Protection 79, no. 2 (2016): 337–44. http://dx.doi.org/10.4315/0362-028x.jfp-15-387.

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ABSTRACT Foodborne transmission of Listeria monocytogenes was first demonstrated through the investigation of the 1981 Maritime Provinces outbreak involving coleslaw. In the following two decades, most listeriosis outbreaks involved foods of animal origin, e.g., deli meats, hot dogs, and soft cheeses. L. monocytogenes serotype 4b, especially epidemic clones I, II, and Ia, were frequently implicated in these outbreaks. However, since 2008 several outbreaks have been linked to diverse types of fresh produce: sprouts, celery, cantaloupe, stone fruit, and apples. The 2011 cantaloupe-associated out
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Mantovani, Nilton César, Magali F. Grando, Aloisio Xavier, and Wagner C. Otoni. "In vitro shoot induction and multiplication from nodal segments of adult Ginkgo biloba plants." Horticultura Brasileira 31, no. 2 (2013): 184–89. http://dx.doi.org/10.1590/s0102-05362013000200003.

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The in vitro performance of herbaceous and woody nodal segments from adult plants and the effect of hydrolyzed casein (HC 500 mg L-1), kinetin (KIN; 6-furfurylaminopurine 0.46 and 4.65 µM) and activated charcoal (AC 1.5 g L-1) were evaluated upon new shoots induction and development, and to establish a system of in vitro propagation from adult plants of Ginkgo biloba. Woody nodal segments did not produce axillary shoots and presented 100% of bacterial and fungal contamination in culture. However, nodal segments from herbaceous shoots were successfully disinfected and displayed high in vitro mo
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French, J. M., J. J. Randall, R. A. Stamler, A. C. Segura, and N. P. Goldberg. "First Report of Anthracnose of Sunflower Sprouts Caused by Colletotrichum acutatum in New Mexico." Plant Disease 97, no. 6 (2013): 838. http://dx.doi.org/10.1094/pdis-08-12-0805-pdn.

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In December 2011, edible sunflower sprouts (Helianthus annus) of two different commercially grown cultivars (Sungrown and Tiensvold) exhibiting stem and cotyledon lesions were submitted to the New Mexico State University Plant Clinic for disease diagnosis. The sample originated from an organic farm in Santa Fe County where the grower utilizes a small indoor growing facility. Stem lesions were elongate, reddish brown, and often constricted, resulting in stem girdling. Lesions on the cotyledons were dark brown with tan centers and round to irregular in shape. In some cases, the entire cotyledon
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LI, YONG, and AZLIN MUSTAPHA. "Simultaneous Detection ofEscherichia coli O157:H7,Salmonella, andShigella in Apple Cider and Produce by a Multiplex PCR†." Journal of Food Protection 67, no. 1 (2004): 27–33. http://dx.doi.org/10.4315/0362-028x-67.1.27.

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With three pairs of primers, a multiplex PCR assay was established for the simultaneous detection of Escherichia coli O157:H7, Salmonella, and Shigella. Under the optimized conditions, the assay yielded a 252-bp product from E. coli O157: H7, a 429-bp product from Salmonella Typhimurium, and a 620-bp product from Shigella flexneri, respectively. When the DNA extraction of multiple target organisms was included in the same reaction, two or three corresponding amplicons of different sizes were observed. In the specificity test, 10 E. coli O157:H7 strains and one E. coli O157:NM strain showed the
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Fang, Zhengwu, Zehao Hou, Shuping Wang, et al. "Transcriptome Analysis Reveals the Accumulation Mechanism of Anthocyanins in Buckwheat (Fagopyrum esculentum Moench) Cotyledons and Flowers." International Journal of Molecular Sciences 20, no. 6 (2019): 1493. http://dx.doi.org/10.3390/ijms20061493.

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Buckwheat (Fagopyrum esculentum) is a valuable crop which can produce multiple human beneficial secondary metabolites, for example, the anthocyanins in sprouts and flowers. However, as the predominant group of visible polyphenols in pigmentation, little is known about the molecular mechanisms underlying the anthocyanin biosynthesis within buckwheat. In this study, a comparative transcriptome analysis of green and red common buckwheat cultivars was carried out through RNA sequencing. Overall, 3727 and 5323 differently expressed genes (DEGs) were identified in flowers and cotyledons, respectivel
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Nguyen, Sanh Du. "Physiological basis of effective controlling Torpedo grass (Panicum repens L.)." Science and Technology Development Journal 16, no. 1 (2013): 34–42. http://dx.doi.org/10.32508/stdj.v16i1.1394.

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Torpedo grass (Panicum repens L.) is a C4 plant species, present on different soil types. The rhizome system and tubers of grass well developed, although flowering but not yet recorded the presence of seeds and seedlings (Yêu cầu tác giả viết lại toàn bộ câu này, không thể sửa được. Lưu ý câu phải có động từ chính). Tuber has high drought tolerance. Regenerative ability of the grass decreases with water content of the tuber. Tuber inability to regenerate shoots when it has water less than 30% of water at first. Repeatedly cut grass will take the tuber reserve depletion, not sufficient to provi
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Oulkar, Dasharath P., Kaushik Banerjee, and Sunil Kulkarni. "Multiresidue Analysis of Plant Growth Regulators in Grapes by Triple Quadrupole and Quadrupole–Time of Flight-Based Liquid Chromatography/Mass Spectrometry." Journal of AOAC INTERNATIONAL 94, no. 6 (2011): 1715–21. http://dx.doi.org/10.5740/jaoacint.sgeoulkar.

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Abstract A selective and sensitive LC-MS/MS method is presented for simultaneous determination of 12 plant growth regulators, viz., indol-3-acetic acid, indol-3-butyric acid, kinetin, zeatin, 6-benzyl aminopurine, gibberellic acid, abscisic acid, chlormequat chloride, forchlorfenuron, paclobutrazole, daminozide, and 2,4-dichlorophenoxy acetic acid, in bud sprouts and grape berries. The sample preparation method involved extraction of homogenized sample (5 g) with 40 mL methanol (80%), and final determination was by LC-MS/MS in the multiple reaction monitoring (MRM) mode with time segmentation
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Taitt, Chris Rowe, Yura S. Shubin, Roselina Angel, and Frances S. Ligler. "Detection of Salmonella enterica Serovar Typhimurium by Using a Rapid, Array-Based Immunosensor." Applied and Environmental Microbiology 70, no. 1 (2004): 152–58. http://dx.doi.org/10.1128/aem.70.1.152-158.2004.

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ABSTRACT The multianalyte array biosensor (MAAB) is a rapid analysis instrument capable of detecting multiple analytes simultaneously. Rapid (15-min), single-analyte sandwich immunoassays were developed for the detection of Salmonella enterica serovar Typhimurium, with a detection limit of 8 × 104 CFU/ml; the limit of detection was improved 10-fold by lengthening the assay protocol to 1 h. S. enterica serovar Typhimurium was also detected in the following spiked foodstuffs, with minimal sample preparation: sausage, cantaloupe, whole liquid egg, alfalfa sprouts, and chicken carcass rinse. Cross
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RANGEL-VARGAS, ESMERALDA, CARLOS A. GÓMEZ-ALDAPA, M. del REFUGIO TORRES-VITELA, ANGÉLICA VILLARRUEL-LÓPEZ, ALBERTO J. GORDILLO-MARTÍNEZ, and JAVIER CASTRO-ROSAS. "Presence and Correlation of Some Enteric Indicator Bacteria, Diarrheagenic Escherichia coli Pathotypes, and Salmonella Serotypes in Alfalfa Sprouts from Local Retail Markets in Pachuca, Mexico." Journal of Food Protection 78, no. 3 (2015): 609–14. http://dx.doi.org/10.4315/0362-028x.jfp-14-229.

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Data on the presence of diarrheagenic Escherichia coli pathotypes (DEPs) in alfalfa sprouts and correlations between the presence of coliform bacteria (CB), fecal coliforms (FC), E. coli, DEPs, and Salmonella in alfalfa sprouts are not available. The presence of and correlations between CB, FC, E. coli, DEPs, and Salmonella in alfalfa sprouts were determined. One hundred sprout samples were collected from retail markets in Pachuca, Hidalgo State, Mexico. The presence of indicator bacteria and Salmonella was determined using conventional culture procedures. DEPs were identified using two multip
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Li, Jian, Tian Chen, Fengzhen Huang, Penghui Dai, Fuxiang Cao, and Meng Li. "Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana." Functional Plant Biology 47, no. 5 (2020): 454. http://dx.doi.org/10.1071/fp19317.

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Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 w
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Jeans, Matthew R., Fiona M. Asigbee, Matthew J. Landry, et al. "Breakfast Consumption in Low-Income Hispanic Elementary School-Aged Children: Associations with Anthropometric, Metabolic, and Dietary Parameters." Nutrients 12, no. 7 (2020): 2038. http://dx.doi.org/10.3390/nu12072038.

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Breakfast consumption is associated with lower obesity prevalence and cardiometabolic risk and higher dietary quality (DQ) in children. Low-income, Hispanic populations are disproportionately affected by obesity and cardiometabolic risks. This study examined the relationship between breakfast consumption groups (BCG) on anthropometric, metabolic, and dietary parameters in predominately low-income, Hispanic children from 16 Texas schools. Cross-sectional data were from TX Sprouts, a school-based gardening, nutrition, and cooking randomized controlled trial. Anthropometric measurements included
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Pogreba-Brown, K., P. O'Connor, J. Matthews, E. Barrett, and M. L. Bell. "Case–case analysis of Campylobacter and Salmonella – using surveillance data for outbreak investigations and monitoring routine risk factors." Epidemiology and Infection 146, no. 15 (2018): 1916–21. http://dx.doi.org/10.1017/s0950268818002200.

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AbstractUtilising routine surveillance data, this study presents a method for generating a baseline comparison that can be used in future foodborne outbreak investigations following a case–case methodology. Salmonella and Campylobacter cases (2012–2015) from Maricopa County, AZ were compared to determine differences in risk factors, symptoms and demographics. For foods and other risk factors, adjusted odds ratios were developed using Campylobacter as the reference. Comparisons were also made for three major Salmonella subtypes, Typhimurium, Enteritidis and Poona as compared with Campylobacter.
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Kang, Tae-Yun, Federico Bocci, Mohit Kumar Jolly, Herbert Levine, José Nelson Onuchic, and Andre Levchenko. "Pericytes enable effective angiogenesis in the presence of proinflammatory signals." Proceedings of the National Academy of Sciences 116, no. 47 (2019): 23551–61. http://dx.doi.org/10.1073/pnas.1913373116.

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Angiogenesis frequently occurs in the context of acute or persistent inflammation. The complex interplay of proinflammatory and proangiogenic cues is only partially understood. Using an experimental model, permitting exposure of developing blood vessel sprouts to multiple combinations of diverse biochemical stimuli and juxtacrine cell interactions, we present evidence that a proinflammatory cytokine, tumor necrosis factor (TNF), can have both proangiogenic and antiangiogenic effects, depending on the dose and the presence of pericytes. In particular, we find that pericytes can rescue and enhan
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BLAGDEN, TRENNA, WILLIAM SCHNEIDER, ULRICH MELCHER, JON DANIELS, and JACQUELINE FLETCHER. "Adaptation and Validation of E-Probe Diagnostic Nucleic Acid Analysis for Detection of Escherichia coli O157:H7 in Metagenomic Data from Complex Food Matrices." Journal of Food Protection 79, no. 4 (2016): 574–81. http://dx.doi.org/10.4315/0362-028x.jfp-15-440.

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ABSTRACT The Centers for Disease Control and Prevention recently emphasized the need for enhanced technologies to use in investigations of outbreaks of foodborne illnesses. To address this need, e-probe diagnostic nucleic acid analysis (EDNA) was adapted and validated as a tool for the rapid, effective identification and characterization of multiple pathogens in a food matrix. In EDNA, unassembled next generation sequencing data sets from food sample metagenomes are queried using pathogen-specific sequences known as electronic probes (e-probes). In this study, the query of mock sequence databa
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HARTANTYO, SRI HARMINDA PAHM, MAN LING CHAU, TSE HSIEN KOH, et al. "Foodborne Klebsiella pneumoniae: Virulence Potential, Antibiotic Resistance, and Risks to Food Safety." Journal of Food Protection 83, no. 7 (2020): 1096–103. http://dx.doi.org/10.4315/jfp-19-520.

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ABSTRACT Gastrointestinal carriage of Klebsiella pneumoniae is a predisposing factor for liver abscess in several Asian countries. To determine whether hypervirulent K. pneumoniae in the gut may be transmitted through food, we screened a range of raw and ready-to-eat retail food by culture and recovered K. pneumoniae in 21% (147 of 698) of samples tested. Based on PCR, no K. pneumoniae isolates carried the rmpA gene linked to community-acquired pyogenic liver abscess, providing no evidence of a link between food and liver disease. However, phenotypic resistance to multiple antibiotic classes w
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de Souza, Larissa Larocca, and Marcelo L. Moretti. "Chemical control of suckers in hazelnut orchards of western Oregon." Weed Technology 34, no. 6 (2020): 863–69. http://dx.doi.org/10.1017/wet.2020.78.

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AbstractHazelnut naturally grows as a multi-stemmed tree. The basal sprouts, known as suckers, grow throughout the season. Suckers are removed to promote a single trunk that facilitates production mechanization and increased yield. In western Oregon, herbicides are the most common method of sucker control, and at least four applications per season are performed in the spring and summer seasons. This study evaluated the efficacy of foliar-applied herbicides currently registered for sucker control in hazelnuts. Season-long and short-term field studies were conducted to assess the efficacy of her
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Zhao, Jiali, Hongyou Li, Juan Huang, et al. "Genome-wide analysis of BBX gene family in Tartary buckwheat (Fagopyrum tataricum)." PeerJ 9 (August 11, 2021): e11939. http://dx.doi.org/10.7717/peerj.11939.

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BBX (B-box), a zinc finger transcription factor with one or two B-box domains, plays an important role in plant photomorphogenesis, growth, and development as well as response to environmental changes. In this study, 28 Tartary buckwheat BBX (FtBBX) genes were identified and screened using a comparison program. Their physicochemical properties, gene structures, conserved motifs, distribution in chromosomal, and phylogeny of the coding proteins, as well as their expression patterns, were analyzed. In addition, multiple collinearity analysis in three monocots and three dicot species illustrated
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Aini, Nabilah, Yulita Nurchayati, and Sri Widodo Agung Suedy. "Pengaruh Perendaman Akar Bibit Bayam Merah (Alternanthera amoena Voss.) dalam Larutan Na2CuEDTA terhadap Pertumbuhan dan Kandungan Antosianin." Bioma : Berkala Ilmiah Biologi 20, no. 2 (2019): 123. http://dx.doi.org/10.14710/bioma.20.2.123-132.

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Red spinach is one of plants containing anthocyanin pigment which has various functions as food and beverage dye, and play a role in the field of health. Anthocyanin biosynthesis in plants is influenced by environmental factors, one of them are nutrients. One of the nutrients needed by plants and part of the anthocyanin biosynthesis pathway is copper (Cu). Cu can be added in the form of a fertilizer such as Na2CuEDTA. This research aims to study the effect of soaking the red spinach in Na2CuEDTA solution on growth and anthocyanin content, and knowing the optimal Na2CuEDTA concentration for gro
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Xian, Dehai, Jing Song, Lingyu Yang, Xia Xiong, Rui Lai, and Jianqiao Zhong. "Emerging Roles of Redox-Mediated Angiogenesis and Oxidative Stress in Dermatoses." Oxidative Medicine and Cellular Longevity 2019 (April 16, 2019): 1–14. http://dx.doi.org/10.1155/2019/2304018.

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Angiogenesis is the process of new vessel formation, which sprouts from preexisting vessels. This process is highly complex and primarily involves several key steps, including stimulation of endothelial cells by growth factors, degradation of the extracellular matrix by proteolytic enzymes, migration and proliferation of endothelial cells, and capillary tube formation. Currently, it is considered that multiple cytokines play a vital role in this process, which consist of proangiogenic factors (e.g., vascular endothelial growth factor, fibroblast growth factors, and angiopoietins) and antiangio
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Kennedy, R., A. J. Wakeham, K. G. Byrne, U. M. Meyer, and F. M. Dewey. "A New Method To Monitor Airborne Inoculum of the Fungal Plant Pathogens Mycosphaerella brassicicola andBotrytis cinerea." Applied and Environmental Microbiology 66, no. 7 (2000): 2996–3003. http://dx.doi.org/10.1128/aem.66.7.2996-3003.2000.

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ABSTRACT We describe a new microtiter immunospore trapping device (MTIST device) that uses a suction system to directly trap air particulates by impaction in microtiter wells. This device can be used for rapid detection and immunoquantification of ascospores ofMycosphaerella brassicicola and conidia of Botrytis cinerea by an enzyme-linked immunosorbent assay (ELISA) under controlled environmental conditions. For ascospores ofM. brassicicola correlation coefficients (r 2) of 0.943 and 0.9514 were observed for the number of MTIST device-impacted ascospores per microtiter well and the absorbance
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JUNEJA, VIJAY K., SUDARSAN MUKHOPADHYAY, DIKE UKUKU, CHENG-AN HWANG, VIVIAN C. H. WU, and HARSHAVARDHAN THIPPAREDDI. "Interactive Effects of Temperature, pH, and Water Activity on the Growth Kinetics of Shiga Toxin–ProducingEscherichia coli O104:H4†." Journal of Food Protection 77, no. 5 (2014): 706–12. http://dx.doi.org/10.4315/0362-028x.jfp-13-387.

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The risk of non-O157 Shiga toxin–producing Escherichia coli strains has become a growing public health concern. Several studies characterized the behavior of E. coli O157:H7; however, no reports on the influence of multiple factors on E. coli O104:H4 are available. This study examined the effects and interactions of temperature (7 to 46°C), pH (4.5 to 8.5), and water activity (aw; 0.95 to 0.99) on the growth kinetics of E. coli O104:H4 and developed predictive models to estimate its growth potential in foods. Growth kinetics studies for each of the 23 variable combinations from a central compo
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Oulkar, Dasharath P., Kaushik Banerjee, Manoj S. Ghaste, et al. "Multiresidue Analysis of Multiclass Plant Growth Regulators in Grapes by Liquid Chromatography/Tandem Mass Spectrometry." Journal of AOAC INTERNATIONAL 94, no. 3 (2011): 968–77. http://dx.doi.org/10.1093/jaoac/94.3.968.

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Abstract A selective and rapid multiresidue analysis method is presented for simultaneous estimation of 12 plant growth regulators (PGRs), namely, auxins (indol-3-acetic acid, indol-3-butyric acid, and naphthyl acetic acid), cytokinins (kinetin, zeatin, and 6-benzyladenine), gibberellic acid (GA3), abscisic acid, and synthetic compounds, namely, forchlorfenuron, paclobutrazole, isoprothiolane, and 2,4-dichlorophenoxy acetic acid (2,4-D) in bud sprouts and grape berries at the development stages of 2–3 and 6–8 mm diameters, which are the critical phases when exogenous application of PGRs may be
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Eid, A. A., L. Hennings, K. Caradine, A. Hodges, and A. M. Safar. "Immature vessel percentage (IVP) as a potential predictive factor for anti-angiogenic therapeutics." Journal of Clinical Oncology 25, no. 18_suppl (2007): 21137. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.21137.

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21137 Background: As opposed to mature blood vessels, the early sprouts (lacking the pericyte coverage) are selectively obliterated after Vascular Endothelial Growth Factor (VEGF) antagonists (e.g. Bevacizumab BV). Microvessel density (MVD) has been widely used to assess tumor vasculature however; it does not give an indication of the degree of vessel maturity since it assesses endothelial cells irrespective to the pericyte coverage. With the introduction of BV for treatment of metastatic colon, lung and breast cancers, predictive markers to guide patient selection are desirable but lacking. M
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