Relevant bibliographies by topics / Multiplex immunohistochemistry

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
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Academic literature on the topic 'Multiplex immunohistochemistry'

Author: Grafiati
Published: 4 June 2021
Last updated: 26 July 2025

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Journal articles on the topic "Multiplex immunohistochemistry"

1

Wistuba, I., E. Parra, and A. Francisco Cruz. "MS17.04 Multiplex Immunohistochemistry." Journal of Thoracic Oncology 14, no. 10 (2019): S191. http://dx.doi.org/10.1016/j.jtho.2019.08.380.

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Sheng, Wenjie, Chaoyu Zhang, T. M. Mohiuddin, et al. "Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy." International Journal of Molecular Sciences 24, no. 4 (2023): 3086. http://dx.doi.org/10.3390/ijms24043086.

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Traditional immunohistochemistry (IHC) has already become an essential method of diagnosis and therapy in cancer management. However, this antibody-based technique is limited to detecting a single marker per tissue section. Since immunotherapy has revolutionized the antineoplastic therapy, developing new immunohistochemistry strategies to detect multiple markers simultaneously to better understand tumor environment and predict or assess response to immunotherapy is necessary and urgent. Multiplex immunohistochemistry (mIHC)/multiplex immunofluorescence (mIF), such as multiplex chromogenic IHC
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Gannot, Gallya, Michael A. Tangrea, Heidi S. Erickson, et al. "Layered Peptide Array for Multiplex Immunohistochemistry." Journal of Molecular Diagnostics 9, no. 3 (2007): 297–304. http://dx.doi.org/10.2353/jmoldx.2007.060143.

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Morrison, Larry E., Mark R. Lefever, Lauren J. Behman, et al. "Brightfield multiplex immunohistochemistry with multispectral imaging." Laboratory Investigation 100, no. 8 (2020): 1124–36. http://dx.doi.org/10.1038/s41374-020-0429-0.

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Forsberg, Peter A., Andrew Hammes, Diana Abbott, et al. "Cellular proliferation by multiplex immunohistochemistry identifies aggressive disease behavior in relapsed multiple myeloma." Leukemia & Lymphoma 60, no. 8 (2019): 2085–87. http://dx.doi.org/10.1080/10428194.2018.1551537.

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Barrow, Emma, D. Gareth Evans, Ray McMahon, James Hill, and Richard Byers. "A comparative study of quantitative immunohistochemistry and quantum dot immunohistochemistry for mutation carrier identification in Lynch syndrome." Journal of Clinical Pathology 64, no. 3 (2010): 208–14. http://dx.doi.org/10.1136/jcp.2010.084418.

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AimsLynch Syndrome is caused by mutations in DNA mismatch repair (MMR) genes. Mutation carrier identification is facilitated by immunohistochemical detection of the MMR proteins MHL1 and MSH2 in tumour tissue and is desirable as colonoscopic screening reduces mortality. However, protein detection by conventional immunohistochemistry (IHC) is subjective, and quantitative techniques are required. Quantum dots (QDs) are novel fluorescent labels that enable quantitative multiplex staining. This study compared their use with quantitative 3,3′-diaminobenzidine (DAB) IHC for the diagnosis of Lynch Sy
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Gupta, Bipin, George Yang, and Marc Key. "Novel Chromogens for Immunohistochemistry in Spatial Biology." Cells 13, no. 11 (2024): 936. http://dx.doi.org/10.3390/cells13110936.

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Spatial relations between tumor cells and host-infiltrating cells are increasingly important in both basic science and clinical research. In this study, we have tested the feasibility of using standard methods of immunohistochemistry (IHC) in a multiplex staining system using a newly developed set of chromogenic substrates for the peroxidase and alkaline phosphatase enzymes. Using this approach, we have developed a set of chromogens characterized by (1) providing fine cellular detail, (2) non-overlapping spectral profiles, (3) an absence of interactions between chromogens, (4) stability when s
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D’Annunzio, Giulia, Luisa Vera Muscatello, Chiara Tugnoli, et al. "Bright-Field Multiplex Immunohistochemistry in Swine PCV2 and PRRSV Lymphadenopathies." Animals 15, no. 12 (2025): 1682. https://doi.org/10.3390/ani15121682.

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Multiplex immunostaining (mIHC) allows the simultaneous detection of multiple antigenic targets within the same tissue section, providing a deeper understanding of spatial variation in cellular distribution. The aim of the present study is to apply this technique to examine the spatial variation of lymphocyte populations in swine lymph nodes during PCV2-SD and PRRSV lymphadenopathy compared with reactive lymphoid hyperplasia. A triple immunohistochemical stain with CD3, CD20 and IBA1 antibodies for the concurrent detection of T lymphocytes, B lymphocytes and macrophages, respectively, was perf
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Semba, Takashi, Atsuko Yonemura, Huaitao Wang, Yilin Tong, Masaya Yamazaki, and Takatsugu Ishimoto. "Abstract 5308: RePROBE: A simple and customizable multiplex immunohistochemistry technique." Cancer Research 85, no. 8_Supplement_1 (2025): 5308. https://doi.org/10.1158/1538-7445.am2025-5308.

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Abstract Background: Multiplex immunostaining techniques have been actively developed and have recently become more prevalent in cancer research. However, most of these techniques require specialized instruments and customized primary antibodies. Here, we established a simple and versatile workflow for multiplex immunohistochemistry (IHC) named RePROBE (Repetitive Primary antibody Replacement and Overlaying images from a Broad range of fluorescence imaging Equipment) that can be readily adopted in many laboratories, providing deeper insight into tumor organization and spatial molecular biology
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Harmsen, Marissa J., Arda Arduç, Maaike C. G. Bleeker, et al. "Increased Angiogenesis and Lymphangiogenesis in Adenomyosis Visualized by Multiplex Immunohistochemistry." International Journal of Molecular Sciences 23, no. 15 (2022): 8434. http://dx.doi.org/10.3390/ijms23158434.

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There is evidence for increased angiogenesis in the (ectopic) endometrium of adenomyosis patients under the influence of vascular endothelial growth factor (VEGF). VEGF stimulates both angiogenesis and lymph-angiogenesis. However, information on lymph vessels in the (ectopic) endometrium of adenomyosis patients is lacking. In this retrospective matched case-control study, multiplex immunohistochemistry was performed on thirty-eight paraffin embedded specimens from premenopausal women who had undergone a hysterectomy at the Amsterdam UMC between 2001 and 2018 to investigate the evidence for (ly
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Dissertations / Theses on the topic "Multiplex immunohistochemistry"

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Akarca, Ayse. "Immunohistochemical studies for identification of biomarkers in haematological malignancies: An approach for potential novel therapeutic targets." Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1127626.

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Lymphoid neoplasms are a subgroup of haematological malignancies that affect circulating lymphocytes. The clinical and biological heterogeneity of lymphoid neoplasms can lead to difficulty in accurate diagnosis in this group of diseases. The advancement in effective and feasible detection platforms has enabled novel biomarkers to improve diagnosis and prognosis, in addition to assist in patient stratification and personalised treatments for these diseases. Although there have been improvements in high-throughput diagnostic techniques, the conventional immunohistochemistry (IHC) remains the mos
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Oguejiofor, Kenneth Kenechukwu. "Prognostic markers in oropharyngeal cancers." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/prognostic-markers-in-oropharyngeal-cancers(fda96224-657d-4049-ae6c-50db33a5388a).html.

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Introduction: Human papillomavirus (HPV) is changing the prevalence, survival and treatment paradigms in oropharyngeal squamous cell carcinoma (OPSCC). Improved survival of patients with HPV positive compared to HPV negative OPSCC has led to trials of treatment de-escalation. Current HPV detection methods are imprecise, therefore standardised assessment of transcriptionally active HPV in OPSCC is required. Furthermore, the differences in immune characteristics and/or the hypoxia response/effects could explain observed differences in prognosis between HPV positive and negative OPSCC. Rigorous H
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Yoon, Diane. "Caractérisation transcriptomique et fonctionnelle des neutrophiles circulants et infiltrants la tumeur dans un modèle murin d'ostéosarcome." Electronic Thesis or Diss., Université Paris Cité, 2025. http://www.theses.fr/2025UNIP5014.

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L'ostéosarcome est le sarcome osseux de haut grade le plus fréquent qui touche principalement les adolescents et les adultes jeunes. Son traitement consiste en une polychimiothérapie et une résection chirurgicale extensive, cependant sa survie reste relativement inchangée depuis les années 1980 malgré les essais cliniques récents de thérapies ciblées, immunothérapies ou traitements basés sur les « Chimeric Antigen Receptor - T cells ». Cet échec d'immunomodulation peut être expliquée par le caractère « froid » de l'ostéosarcome : l'analyse immunohistochimique a montré que les lymphocytes T CD8
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Pomeroy, Ian. "Neocortical lesions in an animal model of multiple sclerosis." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670186.

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Padrão, Ines Liguori. "Adenomas hipofisarios : estudo clinico, morfologico e morfometrico; busca de fatores de prognostico utilizando o metodo imunoistoquimico." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311565.

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Orientador: Patricia Sabino de Matos<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas<br>Made available in DSpace on 2018-08-09T15:50:12Z (GMT). No. of bitstreams: 1 Padrao_InesLiguori_D.pdf: 2489921 bytes, checksum: 62e17012ad64cca8cff1b95a7a39190e (MD5) Previous issue date: 2007<br>Resumo: Adenomas hipofisários (AH) são neoplasias epiteliais geralmente bem diferenciadas e benignas. Manifestam-se clinicamente com sintomas de excesso ou redução hormonal e/ou de massa intracraniana. São classificados conforme aspecto macroscópico/radiológico (macro x mic
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Jorge, Uana Maria Miguel. "Tumores gástricos primários múltiplos e únicos: análise imunohistoquímica comparativa." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5154/tde-29012007-154954/.

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Introdução: Adenocarcinomas gástricos múltiplos primários (AGMP) são encontrados em 3,5% a 10% de todos os pacientes com câncer gástrico. A multiplicidade tumoral é amplamente reconhecida como indicador de predisposição genética para o desenvolvimento de neoplasias Além disso, as rotas de carcinogênese não estão claramente definidas nestes tumores (rota mutadora, ou supressora, ou da E-caderina). Objetivo: avaliar a imunoexpressão de hMLH1, hMSH2, e hMSH6 (rota mutadora), p53 (rota supressora) e E-caderina nos AGMP comparando-se com adenocarcinomas únicos (pareados quanto ao sexo, idade, tipo
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Costa, Marcia Helena Soares. "Estudo da expressão dos receptores do peptídeo insulinotrópico dependente de glicose (GIPR) e do hormônio luteinizante (LHCGR) em tumores e hiperplasias do córtex adrenal." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-11092007-134837/.

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Introdução: Os receptores do peptídeo insulinotrópico dependente de glicose (GIPR) e do hormônio luteinizante (LHCGR) são receptores acoplados à proteína G com amplo padrão de expressão tecidual. A expressão anômala destes receptores tem sido descrita em casos de hiperplasia adrenal macronodular independente de ACTH (AIMAH) e em alguns adenomas, resultando em aumento da secreção hormonal (cortisol, andrógenos e aldosterona) pelo cortex adrenal. O papel destes receptores em outras formas de hiperplasia, como a doença adrenocortical nodular pigmentosa primária (PPNAD), aumento da adrenal associa
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Sekiya, Tomoko. "Análise do gene CDKN1B/p27kip1 em pacientes com neoplasia endócrina múltipla tipo 2." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-26022014-112355/.

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INTRODUÇÃO: Na Neoplasia Endócrina Múltipla tipo 2 (NEM2), o desenvolvimento do Carcinoma Medular de Tireoide (CMT), Feocromocitoma (FEO) e Hiperparatireoidismo primário (HPT) está associado à mutações germinativas ativadoras no proto-oncogene RET. Casos de CMT esporádico podem apresentar mutações somáticas no RET (~40%). A variabilidade fenotípica observada em casos de CMT e FEO familiais associados à NEM2 indica o envolvimento de eventos genéticos adicionais que seriam responsáveis pelas diferenças clínicas observadas nos indivíduos afetados (idade de desenvolvimento, progressão e agressivid
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Books on the topic "Multiplex immunohistochemistry"

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C. M. van der Loos. Immunoenzyme multiple staining methods. Bios Scientific Publishers in association with the Royal Microscopical Society, 1999.

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Yeong, Joe, Bernard A. Fox, Houssein A. Sater, Jaime A. Rodriguez-Canales, and Trevor David McKee, eds. Multiplex Immunohistochemistry/Immunofluorescence Technique: The Potential and Promise for Clinical Application. Frontiers Media SA, 2022. http://dx.doi.org/10.3389/978-2-88974-718-4.

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L, CHRIS VAN DER. Immunoenzyme Multiple Staining Methods. Garland Science, 1999.

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Book chapters on the topic "Multiplex immunohistochemistry"

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Dinevska, Marija, Samuel S. Widodo, and Theo Mantamadiotis. "High-Throughput Multiplex Immunohistochemistry of Glioma Organoids." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3585-8_4.

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Kalra, Jessica, and Jennifer Baker. "Multiplex Immunohistochemistry for Mapping the Tumor Microenvironment." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6759-9_17.

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Nguyen, Thu, Nikolce Kocovski, Sean Macdonald, Han Xian Aw Yeang, Minyu Wang, and Paul J. Neeson. "Multiplex Immunohistochemistry Analysis of Melanoma Tumor-Infiltrating Lymphocytes." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1205-7_39.

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Kang, Wenfei, Anthony Santella, Eric Rosiek, et al. "Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2811-9_15.

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Wrobel, Julia, Coleman Harris, and Simon Vandekar. "Statistical Analysis of Multiplex Immunofluorescence and Immunohistochemistry Imaging Data." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2986-4_8.

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Steele, Keith E., and Charles Brown. "Multiplex Immunohistochemistry for Image Analysis of Tertiary Lymphoid Structures in Cancer." In Tertiary Lymphoid Structures. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8709-2_6.

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Pang, Lokman, Matthias Ernst, and Jennifer Huynh. "Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2811-9_20.

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Dubey, Shikha, Yosep Chong, Beatrice Knudsen, and Shireen Y. Elhabian. "VIMs: Virtual Immunohistochemistry Multiplex Staining via Text-to-Stain Diffusion Trained on Uniplex Stains." In Lecture Notes in Computer Science. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-73284-3_15.

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Lorsakul, Auranuch, Jim Martin, Terry Landowski, et al. "Synthetic Singleplex-Image Generation in Multiplex-Brightfield Immunohistochemistry Digital Pathology Using Deep Generative Models." In Simulation and Synthesis in Medical Imaging. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-44689-4_11.

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Bian, Chang, Beth Phillips, Tim Cootes, and Martin Fergie. "HEMIT: H&E to Multiplex-Immunohistochemistry Image Translation with Dual-Branch Pix2pix Generator." In Lecture Notes in Computer Science. Springer Nature Switzerland, 2025. https://doi.org/10.1007/978-3-031-84525-3_16.

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Conference papers on the topic "Multiplex immunohistochemistry"

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Kawai, Masataka. "Exclusive loss in segmentation for the virtual multiplex immunohistochemistry using multiple singleplex masks." In Digital and Computational Pathology, edited by John E. Tomaszewski and Aaron D. Ward. SPIE, 2025. https://doi.org/10.1117/12.3044651.

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Perrin, Romain, Aurélie Leborgne, Nicolas Passat, Benoît Naegel, and Cédric Wemmert. "Multi-Scale Component-Trees for Enhanced Representation in Multiplex Immunohistochemistry Imaging." In 2024 IEEE International Symposium on Biomedical Imaging (ISBI). IEEE, 2024. http://dx.doi.org/10.1109/isbi56570.2024.10635272.

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Chen, Pingjun, Maria E. Salvatierra, Alejandra Serrano, et al. "Optimizing Cell Recognition in Sarcoma Multiplex Immunohistochemistry Images via Pre-Training Strategies." In 2025 IEEE 22nd International Symposium on Biomedical Imaging (ISBI). IEEE, 2025. https://doi.org/10.1109/isbi60581.2025.10980737.

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Bauer, Daniel R., Mark Lefever, Torsten Leibold, et al. "Abstract 4250: Multispectral imaging of brightfield multiplex immunohistochemistry." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-4250.

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Lorsakul, Auranuch N., Joerg Bredno, Robert L. Ochs, Larry Morrison, and William Day. "Validation of multiplex immunohistochemistry assays using automated image analysis." In Digital Pathology, edited by Metin N. Gurcan and John E. Tomaszewski. SPIE, 2018. http://dx.doi.org/10.1117/12.2293168.

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zheng, Yi, Carla Coltharp, Ryan Dilworth, et al. "Abstract 3832: Optimization strategy for fluorescent multiplex immunohistochemistry tissue staining." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-3832.

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Sobhani, Faranak, Azam Hamidinekoo, Allison H. Hall, et al. "Automated Dcis Identification From Multiplex Immunohistochemistry Using Generative Adversarial Networks." In 2022 IEEE 19th International Symposium on Biomedical Imaging (ISBI). IEEE, 2022. http://dx.doi.org/10.1109/isbi52829.2022.9761413.

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Sobhani, Faranak, Azam Hamidinekoo, Allison H. Hall, et al. "Automated Dcis Identification From Multiplex Immunohistochemistry Using Generative Adversarial Networks." In 2022 IEEE 19th International Symposium on Biomedical Imaging (ISBI). IEEE, 2022. http://dx.doi.org/10.1109/isbi52829.2022.9761413.

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Xu, Siyuan, Guannan Li, Mingxue Gu, and Qingli Li. "Deep Adversarial Network Based Stain Unmixing for Brightfield Multiplex Immunohistochemistry Images." In 2023 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2023. http://dx.doi.org/10.1109/bibm58861.2023.10386010.

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Cooper, Lee A. D., Rami Yacoub, David A. Gutman, et al. "Abstract LB-101: Quantitative imaging of protein expression using multiplex quantum dot immunohistochemistry." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-lb-101.

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Reports on the topic "Multiplex immunohistochemistry"

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Fields, Michael J., Mordechai Shemesh, and Anna-Riitta Fuchs. Significance of Oxytocin and Oxytocin Receptors in Bovine Pregnancy. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568790.bard.

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Oxytocin has multiple actions in bovine reproductive tract and it was our purpose to determine the nature of these actions and their significance for the physiology of bovine reproduction. The bovine oxytocin receptors (OTR) gene was cloned and its expression studied during the cycle and pregnancy. OTR mRNA changed in parallel with OTR with control occurring mainly at the transcriptional level. However, the endocrine regulation of OTR were found in endometrium and cervical mucosa at estrus and at parturition. In both tissues OTR were suppressed in the luteal phase and early pregnancy. Whereas
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