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Journal articles on the topic 'Multiplex immunohistochemistry'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Wistuba, I., E. Parra, and A. Francisco Cruz. "MS17.04 Multiplex Immunohistochemistry." Journal of Thoracic Oncology 14, no. 10 (2019): S191. http://dx.doi.org/10.1016/j.jtho.2019.08.380.

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Sheng, Wenjie, Chaoyu Zhang, T. M. Mohiuddin, et al. "Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy." International Journal of Molecular Sciences 24, no. 4 (2023): 3086. http://dx.doi.org/10.3390/ijms24043086.

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Traditional immunohistochemistry (IHC) has already become an essential method of diagnosis and therapy in cancer management. However, this antibody-based technique is limited to detecting a single marker per tissue section. Since immunotherapy has revolutionized the antineoplastic therapy, developing new immunohistochemistry strategies to detect multiple markers simultaneously to better understand tumor environment and predict or assess response to immunotherapy is necessary and urgent. Multiplex immunohistochemistry (mIHC)/multiplex immunofluorescence (mIF), such as multiplex chromogenic IHC

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3

Gannot, Gallya, Michael A. Tangrea, Heidi S. Erickson, et al. "Layered Peptide Array for Multiplex Immunohistochemistry." Journal of Molecular Diagnostics 9, no. 3 (2007): 297–304. http://dx.doi.org/10.2353/jmoldx.2007.060143.

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4

Morrison, Larry E., Mark R. Lefever, Lauren J. Behman, et al. "Brightfield multiplex immunohistochemistry with multispectral imaging." Laboratory Investigation 100, no. 8 (2020): 1124–36. http://dx.doi.org/10.1038/s41374-020-0429-0.

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Forsberg, Peter A., Andrew Hammes, Diana Abbott, et al. "Cellular proliferation by multiplex immunohistochemistry identifies aggressive disease behavior in relapsed multiple myeloma." Leukemia & Lymphoma 60, no. 8 (2019): 2085–87. http://dx.doi.org/10.1080/10428194.2018.1551537.

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6

Barrow, Emma, D. Gareth Evans, Ray McMahon, James Hill, and Richard Byers. "A comparative study of quantitative immunohistochemistry and quantum dot immunohistochemistry for mutation carrier identification in Lynch syndrome." Journal of Clinical Pathology 64, no. 3 (2010): 208–14. http://dx.doi.org/10.1136/jcp.2010.084418.

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AimsLynch Syndrome is caused by mutations in DNA mismatch repair (MMR) genes. Mutation carrier identification is facilitated by immunohistochemical detection of the MMR proteins MHL1 and MSH2 in tumour tissue and is desirable as colonoscopic screening reduces mortality. However, protein detection by conventional immunohistochemistry (IHC) is subjective, and quantitative techniques are required. Quantum dots (QDs) are novel fluorescent labels that enable quantitative multiplex staining. This study compared their use with quantitative 3,3′-diaminobenzidine (DAB) IHC for the diagnosis of Lynch Sy

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Gupta, Bipin, George Yang, and Marc Key. "Novel Chromogens for Immunohistochemistry in Spatial Biology." Cells 13, no. 11 (2024): 936. http://dx.doi.org/10.3390/cells13110936.

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Spatial relations between tumor cells and host-infiltrating cells are increasingly important in both basic science and clinical research. In this study, we have tested the feasibility of using standard methods of immunohistochemistry (IHC) in a multiplex staining system using a newly developed set of chromogenic substrates for the peroxidase and alkaline phosphatase enzymes. Using this approach, we have developed a set of chromogens characterized by (1) providing fine cellular detail, (2) non-overlapping spectral profiles, (3) an absence of interactions between chromogens, (4) stability when s

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D’Annunzio, Giulia, Luisa Vera Muscatello, Chiara Tugnoli, et al. "Bright-Field Multiplex Immunohistochemistry in Swine PCV2 and PRRSV Lymphadenopathies." Animals 15, no. 12 (2025): 1682. https://doi.org/10.3390/ani15121682.

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Multiplex immunostaining (mIHC) allows the simultaneous detection of multiple antigenic targets within the same tissue section, providing a deeper understanding of spatial variation in cellular distribution. The aim of the present study is to apply this technique to examine the spatial variation of lymphocyte populations in swine lymph nodes during PCV2-SD and PRRSV lymphadenopathy compared with reactive lymphoid hyperplasia. A triple immunohistochemical stain with CD3, CD20 and IBA1 antibodies for the concurrent detection of T lymphocytes, B lymphocytes and macrophages, respectively, was perf

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Semba, Takashi, Atsuko Yonemura, Huaitao Wang, Yilin Tong, Masaya Yamazaki, and Takatsugu Ishimoto. "Abstract 5308: RePROBE: A simple and customizable multiplex immunohistochemistry technique." Cancer Research 85, no. 8_Supplement_1 (2025): 5308. https://doi.org/10.1158/1538-7445.am2025-5308.

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Abstract Background: Multiplex immunostaining techniques have been actively developed and have recently become more prevalent in cancer research. However, most of these techniques require specialized instruments and customized primary antibodies. Here, we established a simple and versatile workflow for multiplex immunohistochemistry (IHC) named RePROBE (Repetitive Primary antibody Replacement and Overlaying images from a Broad range of fluorescence imaging Equipment) that can be readily adopted in many laboratories, providing deeper insight into tumor organization and spatial molecular biology

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Harmsen, Marissa J., Arda Arduç, Maaike C. G. Bleeker, et al. "Increased Angiogenesis and Lymphangiogenesis in Adenomyosis Visualized by Multiplex Immunohistochemistry." International Journal of Molecular Sciences 23, no. 15 (2022): 8434. http://dx.doi.org/10.3390/ijms23158434.

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There is evidence for increased angiogenesis in the (ectopic) endometrium of adenomyosis patients under the influence of vascular endothelial growth factor (VEGF). VEGF stimulates both angiogenesis and lymph-angiogenesis. However, information on lymph vessels in the (ectopic) endometrium of adenomyosis patients is lacking. In this retrospective matched case-control study, multiplex immunohistochemistry was performed on thirty-eight paraffin embedded specimens from premenopausal women who had undergone a hysterectomy at the Amsterdam UMC between 2001 and 2018 to investigate the evidence for (ly

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Oscar, Brück, Sami Blom, Riku Turkki, et al. "Immune Cell Profiling in CML Bone Marrow By Multiplex Immunohistochemistry." Blood 128, no. 22 (2016): 1897. http://dx.doi.org/10.1182/blood.v128.22.1897.1897.

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Abstract Background In most solid tumors, CD8+ cytotoxic T-cells and type 1 T-helper cells are associated with a positive prognosis, but a strong immunosuppressive microenvironment may hamper their effectiveness. This notion has contributed to the development of new immune-activating therapies, such as immune checkpoint inhibitors. Although having demonstrated long-term remissions in many different solid tumor types, immune checkpoint inhibitors have not been evaluated comprehensively in hematological malignancies. In this study, we aimed to characterize the cellular and molecular immunologica

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Silverman, Andrew, Matthew Ingham, Robyn Denise Gartrell, et al. "Interrogating the sarcoma immune microenvironment (iME) using multiplex immunohistochemistry (mIHC)." Journal of Clinical Oncology 36, no. 15_suppl (2018): 11536. http://dx.doi.org/10.1200/jco.2018.36.15_suppl.11536.

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Ugolini, Filippo, Elisa Pasqualini, Sara Simi, Gianna Baroni, and Daniela Massi. "Bright-Field Multiplex Immunohistochemistry Assay for Tumor Microenvironment Evaluation in Melanoma Tissues." Cancers 14, no. 15 (2022): 3682. http://dx.doi.org/10.3390/cancers14153682.

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The tumor microenvironment (TME) plays a crucial role in melanoma development, progression and response to treatment. As many of the most relevant TME cell phenotypes are defined by the simultaneous detection of more than two markers, the bright-field (BF) multiplex immunohistochemistry (IHC) technique has been introduced for the quantitative assessment and evaluation of the relative spatial distances between immune cells and melanoma cells. In the current study, we aimed to validate BF multiplex IHC techniques in the Ventana Discovery Ultra Immunostainer to be applied to the evaluation of the

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Kimura, Alisa, Takahiro Tsujikawa, Hiroki Morimoto, et al. "Abstract 6879: Development of a rapid multiplex immunohistochemistry for characterizing tumor-immune microenvironment." Cancer Research 84, no. 6_Supplement (2024): 6879. http://dx.doi.org/10.1158/1538-7445.am2024-6879.

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Abstract Intratumoral immune profiles have been related to prognosis and therapeutic efficacy; this could result in personalized treatment based on biomarkers. Although techniques for multifactorial evaluation of different tumor-immune microenvironments have been established, their practical implementation in clinical settings still requires further improvement, where the time required for testing needs to be reduced and the technology needs to be clinically validated. To develop a multiplex, quantitative, and rapid tissue evaluation method based on clinically established standard immunohistoc

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Ely, Scott, Peter Forsberg, Ruben Niesvizky, and Tomer Martin Mark. "Plasma cell proliferation by SynKii multiplex immunohistochemistry (mIHC) for clinical use in multiple myeloma (MM)." Journal of Clinical Oncology 34, no. 15_suppl (2016): 8056. http://dx.doi.org/10.1200/jco.2016.34.15_suppl.8056.

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Meseck, Emily K., Bradley L. Njaa, Nicholas J. Haley, Edward H. Park, and Stephen C. Barr. "Use of a Multiplex Polymerase Chain Reaction to Rapidly Differentiate Neospora Caninum from Toxoplasma Gondii in an Adult Dog with Necrotizing Myocarditis and Myocardial Infarct." Journal of Veterinary Diagnostic Investigation 17, no. 6 (2005): 565–68. http://dx.doi.org/10.1177/104063870501700607.

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This report describes a 3-year-old male castrated Mastiff dog that died unexpectedly with locally extensive, acute, necrotizing myocarditis and myocardial infarction. Intralesional protozoal tachyzoites in the affected myocardium were confirmed to be Neospora caninum by a novel multiplex polymerase chain reaction (PCR) and immunohistochemistry. Protozoal organisms were not identified in other tissues by histology, immunohistochemistry, or PCR. The multiplex PCR assay was used to quickly provide preliminary results on fresh myocardium to differentiate N. caninum and Toxoplasma gondii. Neosporos

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Kelly, Aubrey M., Brandon A. Fricker, and Kelly J. Wallace. "Protocol for multiplex fluorescent immunohistochemistry in free-floating rodent brain tissues." STAR Protocols 3, no. 4 (2022): 101672. http://dx.doi.org/10.1016/j.xpro.2022.101672.

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Kryeziu, Kushtrim, Christian H. Bergsland, Tormod K. Guren, Anita Sveen, and Ragnhild A. Lothe. "Multiplex immunohistochemistry of metastatic colorectal cancer and ex vivo tumor avatars." Biochimica et Biophysica Acta (BBA) - Reviews on Cancer 1877, no. 1 (2022): 188682. http://dx.doi.org/10.1016/j.bbcan.2022.188682.

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19

Aljakna, Aleksandra, Estelle Lauer, Sébastien Lenglet, et al. "Multiplex quantitative imaging of human myocardial infarction by mass spectrometry-immunohistochemistry." International Journal of Legal Medicine 132, no. 6 (2018): 1675–84. http://dx.doi.org/10.1007/s00414-018-1813-9.

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20

Chen, Ting, and Chukka Srinivas. "Group sparsity model for stain unmixing in brightfield multiplex immunohistochemistry images." Computerized Medical Imaging and Graphics 46 (December 2015): 30–39. http://dx.doi.org/10.1016/j.compmedimag.2015.04.001.

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21

MASUGI, Yohei. "Pancreatic cancer stromal heterogeneity characterized by multiplex immunohistochemistry-based image analyses." Suizo 38, no. 1 (2023): 28–36. http://dx.doi.org/10.2958/suizo.38.28.

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22

Machuca-Ostos, Mercedes, Tim de Martines, Kanako Yoshimura, et al. "Applications of Multiplex Immunohistochemistry in Evaluating Spatiotemporal Heterogeneity of T Cells." Immuno 5, no. 1 (2025): 7. https://doi.org/10.3390/immuno5010007.

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T cell phenotypes and kinetics are emerging as crucial factors associated with immunotherapeutic responses in a wide range of solid cancer types. However, challenges remain in understanding the spatial and temporal profiles of T cells with differential phenotypes due to difficulties in single-cell analysis with preserved tissue structures. Here, we provide an optimized 12-marker multiplex immunohistochemical (IHC) panel and single-cell-based quantitative assessment to identify the spatial distributions of T cell phenotypes in formalin-fixed paraffin-embedded sections. This panel revealed diffe

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23

Shidham, Vinod B., and Lester J. Layfield. "Cell-blocks and immunohistochemistry." Cytojournal 18 (January 30, 2021): 2. http://dx.doi.org/10.25259/cytojournal_83_2020.

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The interpretation of results on immunostained cell-block sections has to be compared with the cumulative published data derived predominantly from formalin-fixed paraffin-embedded (FFPE) tissue sections. Because of this, it is important to recognize that the fixation and processing protocol should not be different from the routinely processed FFPE surgical pathology tissue. Exposure to non-formalin fixatives or reagents may interfere with the diagnostic immunoreactivity pattern. The immunoprofile observed on such cell-blocks, which are not processed in a manner similar to the surgical patholo

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Gorris, Mark A. J., Altuna Halilovic, Katrin Rabold, et al. "Eight-Color Multiplex Immunohistochemistry for Simultaneous Detection of Multiple Immune Checkpoint Molecules within the Tumor Microenvironment." Journal of Immunology 200, no. 1 (2017): 347–54. http://dx.doi.org/10.4049/jimmunol.1701262.

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25

Ely, Scott, Peter Forsberg, Ihsane Ouansafi, et al. "Cellular Proliferation by Multiplex Immunohistochemistry Identifies High-Risk Multiple Myeloma in Newly Diagnosed, Treatment-Naive Patients." Clinical Lymphoma Myeloma and Leukemia 17, no. 12 (2017): 825–33. http://dx.doi.org/10.1016/j.clml.2017.09.010.

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Gartrell, Robyn Denise, Douglas Kanter Marks, Thomas Hart, et al. "Characterizing the tumor microenvironment (TME) in primary melanomas using multiplex immunohistochemistry (mIHC)." Journal of Clinical Oncology 35, no. 15_suppl (2017): 9580. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.9580.

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9580 Background: Biomarkers are needed in primary melanoma to risk stratify for adjuvant trials. High levels of infiltrating cytotoxic (CD8+) T lymphocytes (CTLs) and low levels of CD68+ macrophages (MΦ) may correlate with prolonged survival but quantification methods are not standardized for clinical practice. HLA-DR is a marker of MΦ activation not expressed by suppressor myeloid cells. A novel pathology technique using mIHC allows for quantitative and spatial analysis of immune cell subsets. Methods: In a pilot set of stage II/III primary melanomas from Columbia University Medical Center (n

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27

Tsujikawa, Takahiro, Rohan N. Borkar, Vahid Azimi, et al. "Multiplex immunohistochemistry for immune profiling of HPV-associated head and neck cancer." Journal for ImmunoTherapy of Cancer 3, Suppl 2 (2015): P419. http://dx.doi.org/10.1186/2051-1426-3-s2-p419.

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28

Tan, Wei Chang Colin, Sanjna Nilesh Nerurkar, Hai Yun Cai, et al. "Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy." Cancer Communications 40, no. 4 (2020): 135–53. http://dx.doi.org/10.1002/cac2.12023.

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29

Chan, Ronald C. K., Joshua J. X. Li, W. Yeung, and Anthony W. H. Chan. "Virtual multiplex immunohistochemistry: Application on cell block of effusion and aspiration cytology." Diagnostic Cytopathology 48, no. 5 (2020): 417–23. http://dx.doi.org/10.1002/dc.24344.

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30

Morrison, Larry E., Tania M. Larrinaga, Brian D. Kelly, Mark R. Lefever, Rachel C. Beck, and Daniel R. Bauer. "Concurrent Viewing of H&E and Multiplex Immunohistochemistry in Clinical Specimens." Diagnostics 15, no. 2 (2025): 164. https://doi.org/10.3390/diagnostics15020164.

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Background/Objectives: Performing hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) on the same specimen slide provides advantages that include specimen conservation and the ability to combine the H&E context with biomarker expression at the individual cell level. We previously used invisible deposited chromogens and dual-camera imaging, including monochrome and color cameras, to implement simultaneous H&E and IHC. Using this approach, conventional H&E staining could be simultaneously viewed in color on a computer monitor alongside a monochrome video of the in

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Mandelkow, Tim, Gisa Mehring, Elena Bady, et al. "Abstract 5438: The combination of artificial intelligence and BLEACH&STAIN multiplex fluorescence immunohistochemistry facilitates automated prostate and breast cancer detection." Cancer Research 83, no. 7_Supplement (2023): 5438. http://dx.doi.org/10.1158/1538-7445.am2023-5438.

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Abstract Background: Automated prognosis marker assessment in prostate and breast cancer using immunohistochemistry is currently hampered by the lack of a reliable differentiation between benign and malignant glands. To evaluate the patient’s risk in routine clinical practice in prostate cancer prognosis parameters such as the Gleason grading, that are accompanied by a high interobserver variability are used. In breast cancer multi-gene panels are used that are influenced by fluctuating tumor purity. A reproducible prognostic evaluation is lacking in both tumor entities. Design: To enable auto

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Melvin, S., and M. Nam. "Can multiplexing Immunohistochemistry for PD-L1, CD68, and CD3 improve scoring for “Keytruda®” Indication?" American Journal of Clinical Pathology 162, Supplement_1 (2024): S95. http://dx.doi.org/10.1093/ajcp/aqae129.212.

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Abstract Introduction/Objective FDA-approved companion diagnostics immunohistochemistry scoring for PD-L1 is essential for Keytruda ® treatment in patients with NSCLC. The challenge with PD-L1 testing alone is false positive PD-L1 expression score due to alveolar macrophage infiltration. Multiplexing with PD-L1, CD68, and CD3 could help alleviate this issue. By viewing PD-L1 expression on tumor cells, CD68 expression on macrophages, and the presence of CD3+ T cells on a single slide, pathologists will be able to see the presence of an immune microenvironment (high PD-L1 expression with abundan

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Blessin, Niclas C., Tim Mandelkow, Elena Bady, et al. "Abstract 483: Automated Ki67-LI assessment in prostate cancer using artificial intelligence in multiplex fluorescence immunohistochemistry." Cancer Research 82, no. 12_Supplement (2022): 483. http://dx.doi.org/10.1158/1538-7445.am2022-483.

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Abstract The Ki67 labeling index (Ki67-LI) is a strong prognostic marker in prostate cancer. Its analysis requires cumbersome manual quantification of Ki67 immunostaining in at least 200 tumor cells. To enable automated Ki67-LI assessment in routine clinical practice, we have developed and validated a framework for automated Ki67-LI quantification, which comprises three different artificial intelligence analysis steps and an algorithm for cell-distance analysis of multiplex fluorescence immunohistochemistry staining. The prognostic impact of the Ki67-LI was tested on a tissue microarray (TMA)

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Pacia, Emmanuel, Xun Li, Ju Young Kim, et al. "47 Pathologists enhance interpretation of automated multiplex immunohistochemistry assays in cancer immunotherapy trials." Journal for ImmunoTherapy of Cancer 8, Suppl 3 (2020): A50. http://dx.doi.org/10.1136/jitc-2020-sitc2020.0047.

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BackgroundMultiplex fluorescence immunohistochemistry (mFIHC) enables simultaneous detection of multiple biomarkers on a single tissue section. Spatial patterns and differential expression of immune- and tumor cell biomarkers serve as powerful predictors of immunotherapies. In a recent meta-analyses of 8135 patients treated with PD1/L1 pathway blockers, mFIHC was found to provide highest predictive power (P<0.05) amongst commonly utilized biomarker modalities, namely, PD-L1 IHC, Tumor Mutation Burden and Gene Expression Profiling alone. [Lu et al., JAMA Oncol 2019]. As biomarkers in mFIHC a

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Tsujikawa, Takahiro, Sushil Kumar, Rohan N. Borkar, et al. "Quantitative Multiplex Immunohistochemistry Reveals Myeloid-Inflamed Tumor-Immune Complexity Associated with Poor Prognosis." Cell Reports 19, no. 1 (2017): 203–17. http://dx.doi.org/10.1016/j.celrep.2017.03.037.

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Gell, Katherine, Katherine Ankenbauer, Vincent Quoc-Huy Trinh, Brenda Jarvis, and Kathleen E. DelGiorno. "Su1300: USING MULTIPLEX IMMUNOHISTOCHEMISTRY TO MAP METAPLASTIC CELL POPULATIONS IN PANCREATIC INTRAEPITHELIAL NEOPLASMS." Gastroenterology 169, no. 1 (2025): S—735—S—736. https://doi.org/10.1016/s0016-5085(25)02648-4.

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Slik, Khadija. "Digital Pathology. Role of molecular diagnostics in cancers; multiple immunohistochemistry." Academy Journal For Basic and Applied Sciences 7, no. 1 (2025): 1–4. https://doi.org/10.5281/zenodo.15274710.

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Multiplexed platforms have become a standard feature of modern medicine in the field of histopathology in recent years. They have evolved into powerful technologies that enable image analysis of tumor tissues from formalin- fixed paraffin- embedded specimens, aiming for better assessment of morphology and distinctive alterations at the molecular level of the patient’s sample, which is critical for the pathologist’s diagnosis and classification, with significant implications for the following therapeutic options. And also, in order to gain a better understanding of the tumor microen

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Sorrelle, Noah, Debolina Ganguly, Adrian T. A. Dominguez, et al. "Improved Multiplex Immunohistochemistry for Immune Microenvironment Evaluation of Mouse Formalin-Fixed, Paraffin-Embedded Tissues." Journal of Immunology 202, no. 1 (2018): 292–99. http://dx.doi.org/10.4049/jimmunol.1800878.

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Peter, Simon, Valery Volk, Charlotte Hoffmann, et al. "Characterization and clinical correlation of the immune contexture in intrahepatic cholangiocarcinoma using multiplex immunohistochemistry." Journal of Hepatology 77 (July 2022): S927. http://dx.doi.org/10.1016/s0168-8278(22)02136-5.

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Arnold, Stephanie, Sarah Watts, Emrys Kirkman, Clive P. Page, and Simon C. Pitchford. "Single and Multiplex Immunohistochemistry to Detect Platelets and Neutrophils in Rat and Porcine Tissues." Methods and Protocols 5, no. 5 (2022): 71. http://dx.doi.org/10.3390/mps5050071.

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Platelet–neutrophil complexes (PNCs) occur during the inflammatory response to trauma and infections, and their interactions enable cell activation that can lead to tissue destruction. The ability to identify the accumulation and tissue localisation of PNCs is necessary to further understand their role in the organs associated with blast-induced shock wave trauma. Relevant experimental lung injury models often utilise pigs and rats, species for which immunohistochemistry protocols to detect platelets and neutrophils have yet to be established. Therefore, monoplex and multiplex immunohistochemi

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Pugh, Matthew, Ayse U. Akarka, Kelly Hunter, Stefan Dojcinov, and Teresa Marafioti. "Multiplex immunohistochemistry in lymphoma pathology: a research tool for study of the immune microenvironment." Diagnostic Histopathology 26, no. 9 (2020): 407–20. http://dx.doi.org/10.1016/j.mpdhp.2020.07.002.

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Ely, S., G. Lee, L. Menard, et al. "Multiplex chromogenic Immunohistochemistry (IHC) for spatial analysis of checkpoint-positive tumour infiltrating lymphocytes (TILs)." Annals of Oncology 30 (October 2019): v40. http://dx.doi.org/10.1093/annonc/mdz239.039.

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Zito Marino, Federica, Giulio Rossi, Immacolata Cozzolino, et al. "Multiplex fluorescence in situ hybridisation to detect anaplastic lymphoma kinase and ROS proto-oncogene 1 receptor tyrosine kinase rearrangements in lung cancer cytological samples." Journal of Clinical Pathology 73, no. 2 (2019): 96–101. http://dx.doi.org/10.1136/jclinpath-2019-206152.

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AimsSeveral predictive biomarkers of response to specific inhibitors have become mandatory for the therapeutic choice in non-small-cell lung cancer (NSCLC). In most lung cancer patients, the biological materials available to morphological and molecular diagnosis are exclusively cytological samples and minimum tumour wastage is necessary. Multiplex fluorescence in situ hybridisation (mFISH) to detect simultaneously ALK-rearrangement and ROS1-rearrangement on a single slide could be useful in clinical practice to save cytological samples for further molecular analysis. In this study, we aim to v

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Hu, Weixian. "Multiplex immunohistochemistry/immunofluorescence is superior to tumor mutational burden and PD‐L1 immunohistochemistry for predicting response to anti‐PD‐1/PD‐L1 immunotherapy." Thoracic Cancer 11, no. 1 (2019): 3–5. http://dx.doi.org/10.1111/1759-7714.13233.

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Gérus-Durand, Marie, Elena Baranova, Maroua Tliba, et al. "Abstract 3871: Solid tumor analytical validation of a T-regulatory immunohistochemistry multiplex for clinical studies." Cancer Research 82, no. 12_Supplement (2022): 3871. http://dx.doi.org/10.1158/1538-7445.am2022-3871.

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Abstract While classic, chromogenic immunohistochemistry (IHC) remains a mainstay in clinical diagnostics, it can be limiting in the era of immuno-oncology. In the field of immuno-oncology, where access to tissue samples may be limited, there is a need for an accurate interpretation of the sociology and in-depth phenotyping of the cells in the tumor microenvironment that requires a higher level of targets to investigate. Cytotoxic T cells are the main effector cells against tumors. Conversely, T-regulatory cells play a more pro-tumor role by suppressing cytotoxic T cell activation. The importa

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Sivagnanam, Shamilene, Courtney M. Betts, Nell Kirchberger, Konjit Betre, and Lisa M. Coussens. "Abstract 4293: Strategies and resources for applying a quantitative multiplex IHC imaging workflow to characterize immune contexture in solid tumors." Cancer Research 83, no. 7_Supplement (2023): 4293. http://dx.doi.org/10.1158/1538-7445.am2023-4293.

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Abstract Conventional immunohistochemistry (IHC) is a standardized diagnostic technique used in tissue pathology. However, the capacity to label only one marker per tissue section is a critical limitation. Multiplex immunohistochemistry (mIHC) is a powerful imaging technique used in basic, translational research and clinical settings to simultaneously detect the expression of multiple epitopes in a single formalin-fixed paraffin-embedded (FFPE) tissue section, allowing for characterization and quantification of cells while maintaining their spatial location. This technique allows for a compreh

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De Lorenzo, Cíntia, Caroline P. de Andrade, Verônica S. L. Machado, et al. "Piglet colibacillosis diagnosis based on multiplex polymerase chain reaction and immunohistochemistry of paraffin-embedded tissues." Journal of Veterinary Science 19, no. 1 (2018): 27. http://dx.doi.org/10.4142/jvs.2018.19.1.27.

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Park, Soon Sik, John Williams Gillespie, Bill James, and Michael Lebowitz. "Multiplex layered immunohistochemistry to predict response of HER2-positive breast cancer patients to trastuzumab therapy." Journal of Clinical Oncology 30, no. 27_suppl (2012): 136. http://dx.doi.org/10.1200/jco.2012.30.27_suppl.136.

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136 Background: Since only 40% of HER2-positive breast cancer patients respond to trastuzumab therapy, there is a great clinical need for a test that will better predict which patients would be responders to trastuzumab therapy. Since no single biomarker will likely improve identification of responders, this study will determine the efficacy of multiple biomarkers to predict response of patients to trastuzumab therapy. Our company has developed a protein analysis platform, layered immunohistochemistry (L-IHC), for the analysis of multiple protein biomarkers from a single FFPE tissue section wh

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Bâeta, A. M., G. A. Putzu, D. Figarella-Branger, E. Cassote, and J. F. Pellissier. "Immunohistochemistry, immunoblot and PCR multiplex results in 16 patients with cramp/myalgia and/or rhabdomyolysis." Neuromuscular Disorders 6, no. 2 (1996): S30. http://dx.doi.org/10.1016/0960-8966(96)89049-3.

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Maiques, Oscar, and Victoria Sanz-Moreno. "Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human." STAR Protocols 3, no. 4 (2022): 101879. http://dx.doi.org/10.1016/j.xpro.2022.101879.

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