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Journal articles on the topic 'Multiplexed fluorescence imaging'

Author: Grafiati

Published: 29 April 2023

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1

Cappella, Paolo, and Fabio Gasparri. "Highly Multiplexed Phenotypic Imaging for Cell Proliferation Studies." Journal of Biomolecular Screening 19, no. 1 (2013): 145–57. http://dx.doi.org/10.1177/1087057113495712.

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The application of multiplexed imaging technologies in phenotypic drug discovery (PDD) enables profiling of complex cellular perturbations in response to drug treatment. High-content analysis (HCA) is among the most pursued approaches in PDD, with a proven capability to identify compounds with a given cellular mechanism of action (MOA), as well as to unveil unexpected drug cellular activities. The ability of fluorescent image-based cytometric techniques to dissect the phenotypic heterogeneity of cell populations depends on the degree of multiplexing achievable. At present, most high-content as

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2

Liu, Hsiou-Yuan, Jingshan Zhong, and Laura Waller. "Multiplexed phase-space imaging for 3D fluorescence microscopy." Optics Express 25, no. 13 (2017): 14986. http://dx.doi.org/10.1364/oe.25.014986.

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3

Luo, Teng, Ting Zhou, Yihua Zhao, Liwei Liu, and Junle Qu. "Multiplexed fluorescence lifetime imaging by concentration-dependent quenching." Journal of Materials Chemistry B 6, no. 13 (2018): 1912–19. http://dx.doi.org/10.1039/c8tb00095f.

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4

Ko, Jina, Juhyun Oh, Maaz S. Ahmed, Jonathan C. T. Carlson, and Ralph Weissleder. "Ultra‐fast Cycling for Multiplexed Cellular Fluorescence Imaging." Angewandte Chemie 132, no. 17 (2020): 6906–13. http://dx.doi.org/10.1002/ange.201915153.

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Ko, Jina, Juhyun Oh, Maaz S. Ahmed, Jonathan C. T. Carlson, and Ralph Weissleder. "Ultra‐fast Cycling for Multiplexed Cellular Fluorescence Imaging." Angewandte Chemie International Edition 59, no. 17 (2020): 6839–46. http://dx.doi.org/10.1002/anie.201915153.

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6

Gamber, Kevin, Arne Christians, Spencer Schwarz, et al. "Quantitative Immune Profiling of Human Tumor Tissues with Multiplexed ChipCytometry." Journal of Immunology 206, no. 1_Supplement (2021): 68.05. http://dx.doi.org/10.4049/jimmunol.206.supp.68.05.

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Abstract Cancer therapies that rely on manipulating or engineering immune cells have shown much promise in recent years for effectively combating a broad array of cancer types. In order to determine the effectiveness of such therapies, it is necessary to accurately profile the complement of immune cell types present in the tumor microenvironment. Methods to reliably obtain immune cell signatures require the combination of powerful resolution to discriminate cell boundaries, broad dynamic range to capture markers of varying expression levels, and accurate cell segmentation across a wide range o

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7

Mizuno, T., E. Hase, T. Minamikawa, et al. "Full-field fluorescence lifetime dual-comb microscopy using spectral mapping and frequency multiplexing of dual-comb optical beats." Science Advances 7, no. 1 (2021): eabd2102. http://dx.doi.org/10.1126/sciadv.abd2102.

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Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust to photobleaching. However, since most FLIMs are based on point-to-point measurements, mechanical scanning of a focal spot is needed for forming an image, which hampers rapid imaging. Here, we demonstrate scan-less full-field FLIM based on a one-to-one correspondence between two-dimensional (2D) image pixels and frequency-multiplexed radio frequency (RF) si

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Xu, Jian, Tianyue Zhang, Shenyu Yang, et al. "Plasmonic Nanoprobes for Multiplexed Fluorescence-Free Super-Resolution Imaging." Advanced Optical Materials 6, no. 20 (2018): 1800432. http://dx.doi.org/10.1002/adom.201800432.

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9

Lv, Yanlu, Jiulou Zhang, Dong Zhang, Wenjuan Cai, Nanguang Chen, and Jianwen Luo. "In vivosimultaneous multispectral fluorescence imaging with spectral multiplexed volume holographic imaging system." Journal of Biomedical Optics 21, no. 6 (2016): 060502. http://dx.doi.org/10.1117/1.jbo.21.6.060502.

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10

Keshri, Puspam, Bin Zhao, Tianfa Xie, et al. "Quantitative and Multiplexed Fluorescence Lifetime Imaging of Intercellular Tensile Forces." Angewandte Chemie 133, no. 28 (2021): 15676–83. http://dx.doi.org/10.1002/ange.202103986.

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11

Keshri, Puspam, Bin Zhao, Tianfa Xie, et al. "Quantitative and Multiplexed Fluorescence Lifetime Imaging of Intercellular Tensile Forces." Angewandte Chemie International Edition 60, no. 28 (2021): 15548–55. http://dx.doi.org/10.1002/anie.202103986.

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12

Barash, Eugene, Sean Dinn, Christopher Sevinsky, and Fiona Ginty. "Multiplexed Analysis of Proteins in Tissue Using Multispectral Fluorescence Imaging." IEEE Transactions on Medical Imaging 29, no. 8 (2010): 1457–62. http://dx.doi.org/10.1109/tmi.2010.2045005.

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13

Bhagavatula, Sharath, Devon Thompson, Sebastian W. Ahn, et al. "A Miniaturized Platform for Multiplexed Drug Response Imaging in Live Tumors." Cancers 13, no. 4 (2021): 653. http://dx.doi.org/10.3390/cancers13040653.

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By observing the activity of anti-cancer agents directly in tumors, there is potential to greatly expand our understanding of drug response and develop more personalized cancer treatments. Implantable microdevices (IMD) have been recently developed to deliver microdoses of chemotherapeutic agents locally into confined regions of live tumors; the tissue can be subsequently removed and analyzed to evaluate drug response. This method has the potential to rapidly screen multiple drugs, but requires surgical tissue removal and only evaluates drug response at a single timepoint when the tissue is ex

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14

Xiao, Lu, Joshua Labaer, and Jia Guo. "Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide." Cells 10, no. 6 (2021): 1277. http://dx.doi.org/10.3390/cells10061277.

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Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations. However, the current multiplexed RNA imaging technologies are limited by their relatively low sensitivity or specificity, which hinders their applications in studying highly autofluorescent tissues, such as formalin-fixed paraffin-embedded (FFPE) tissues. To address this issue, here we develop a multiplexed in situ RNA profiling approach with a high sensitivity and specificity. In this approach, transcripts are first hybridi

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15

Wegner, K. David, and Niko Hildebrandt. "Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors." Chemical Society Reviews 44, no. 14 (2015): 4792–834. http://dx.doi.org/10.1039/c4cs00532e.

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Kuang, Yu, Guillem Pratx, Magdalena Bazalova, Bowen Meng, Jianguo Qian, and Lei Xing. "First Demonstration of Multiplexed X-Ray Fluorescence Computed Tomography (XFCT) Imaging." IEEE Transactions on Medical Imaging 32, no. 2 (2013): 262–67. http://dx.doi.org/10.1109/tmi.2012.2223709.

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17

Jin, Zhao-Hui, Atsushi B. Tsuji, Mélissa Degardin, Pascal Dumy, Didier Boturyn, and Tatsuya Higashi. "Multiplexed Imaging Reveals the Spatial Relationship of the Extracellular Acidity-Targeting pHLIP with Necrosis, Hypoxia, and the Integrin-Targeting cRGD Peptide." Cells 11, no. 21 (2022): 3499. http://dx.doi.org/10.3390/cells11213499.

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pH (low) insertion peptides (pHLIPs) have been developed for cancer imaging and therapy targeting the acidic extracellular microenvironment. However, the characteristics of intratumoral distribution (ITD) of pHLIPs are not yet fully understood. This study aimed to reveal the details of the ITD of pHLIPs and their spatial relationship with other tumor features of concern. The fluorescent dye-labeled pHLIPs were intravenously administered to subcutaneous xenograft mouse models of U87MG and IGR-OV1 expressing αVβ3 integrins (using large necrotic tumors). The αVβ3 integrin-targeting Cy5.5-RAFT-c(-

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18

Moffitt, Jeffrey R., Junjie Hao, Guiping Wang, Kok Hao Chen, Hazen P. Babcock, and Xiaowei Zhuang. "High-throughput single-cell gene-expression profiling with multiplexed error-robust fluorescence in situ hybridization." Proceedings of the National Academy of Sciences 113, no. 39 (2016): 11046–51. http://dx.doi.org/10.1073/pnas.1612826113.

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Image-based approaches to single-cell transcriptomics, in which RNA species are identified and counted in situ via imaging, have emerged as a powerful complement to single-cell methods based on RNA sequencing of dissociated cells. These image-based approaches naturally preserve the native spatial context of RNAs within a cell and the organization of cells within tissue, which are important for addressing many biological questions. However, the throughput of these image-based approaches is relatively low. Here we report advances that lead to a drastic increase in the measurement throughput of m

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Luo, Teng, Danying Lin, Ting Zhou, Yuan Lu, Shaoxiong Liu, and Junle Qu. "Identification and characterization of different tissues in blood vessel by multiplexed fluorescence lifetimes." Analyst 143, no. 10 (2018): 2243–48. http://dx.doi.org/10.1039/c8an00392k.

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Herein, fluorescence lifetime imaging microscopy (FLIM) was used to directly measure eosin fluorescence lifetimes from H&E-stained umbilical artery, and a further utilization of eosin for high-content and multi-target analysis was proposed for the first time.

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20

Xiao, Lu, and Jia Guo. "Multiplexed single-cell in situ RNA analysis by reiterative hybridization." Analytical Methods 7, no. 17 (2015): 7290–95. http://dx.doi.org/10.1039/c5ay00500k.

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A novel method to quantify the identities, positions, and copy numbers of a large number of different RNA species in single cells has been developed by reiterative cycles of target hybridization, fluorescence imaging and photobleaching.

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21

Sograte-Idrissi, Shama, Nazar Oleksiievets, Sebastian Isbaner, et al. "Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors." Cells 8, no. 1 (2019): 48. http://dx.doi.org/10.3390/cells8010048.

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DNA point accumulation for imaging in nanoscale topography (PAINT) is a rapidly developing fluorescence super-resolution technique, which allows for reaching spatial resolutions below 10 nm. It also enables the imaging of multiple targets in the same sample. However, using DNA-PAINT to observe cellular structures at such resolution remains challenging. Antibodies, which are commonly used for this purpose, lead to a displacement between the target protein and the reporting fluorophore of 20–25 nm, thus limiting the resolving power. Here, we used nanobodies to minimize this linkage error to ~4 n

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22

Tewson, Paul H., Anne Marie Quinn, and Thomas E. Hughes. "A Multiplexed Fluorescent Assay for Independent Second-Messenger Systems." Journal of Biomolecular Screening 18, no. 7 (2013): 797–806. http://dx.doi.org/10.1177/1087057113485427.

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There is a growing need in drug discovery and basic research to measure multiple second-messenger components of cell signaling pathways in real time and in relevant tissues and cell types. Many G-protein–coupled receptors activate the heterotrimeric protein, Gq, which in turn activates phospholipase C (PLC). PLC cleaves phosphatidylinositol 4,5-bisphosphate (PIP2) to produce two second messengers: diacylglycerol (DAG), which remains in the plasma membrane, and inositol triphosphate (IP3), which diffuses through the cytosol to release stores of intracellular calcium ions (Ca2+). Our goal was to

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23

Zhao, Ming, Yu Li, and Leilei Peng. "Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging." Optics Express 22, no. 9 (2014): 10221. http://dx.doi.org/10.1364/oe.22.010221.

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24

Maryu, Gembu, Michiyuki Matsuda, and Kazuhiro Aoki. "Multiplexed Fluorescence Imaging of ERK and Akt Activities and Cell-cycle Progression." Cell Structure and Function 41, no. 2 (2016): 81–92. http://dx.doi.org/10.1247/csf.16007.

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25

Northcutt, Adam J., Arne Christians, Jason T. Forys, Thomas D. Campbell, and Crystal L. Winkeler. "Quantitative immune profiling of human tumor tissues with multiplexed ChipCytometry." Journal of Immunology 204, no. 1_Supplement (2020): 159.10. http://dx.doi.org/10.4049/jimmunol.204.supp.159.10.

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Abstract Objective Many immune cell profiling methods have demonstrated limits on the number of biomarkers that can be reliably assayed from the same cell; to remedy this, we present here ChipCytometry, an imaging technology for quantitative immune profiling of cells and sectioned tissues. Methods ChipCytometry is a fluorescence-based imaging system that utilizes multiplexed immuno-fluorescence staining in combination with high-dynamic-range (HDR) imaging to facilitate quantitative phenotyping of individual cells within tissue samples. Employing this technology to profile metastatic and primar

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26

Potier, Guillaume, Aurélie Doméné, and Perrine Paul-Gilloteaux. "A flexible open-source processing workflow for multiplexed fluorescence imaging based on cycles." F1000Research 11 (September 29, 2022): 1121. http://dx.doi.org/10.12688/f1000research.124990.1.

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Background Multiplexing tissue imaging is developing as a complement for single cell analysis, bringing the spatial information of cells in tissue in addition to multiple parameters measurements. More and more commercial or home-made systems are available. These techniques allow the imaging of tens of fluorescent reporters, where the spectral overlap is solved by imaging by cycles the fluorophores using microfluidics to change the reporters between each cycle. Methods For several systems, the acquisition system coupled to the microfluidic system is a wide field microscope, and the acquisition

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27

WANG Da-hui, 王大辉, 钱航 QIAN Hang, 赵学庆 ZHAO Xue-qing, et al. "Automatic alignment of multiplexed beams of excimer laser system based on fluorescence imaging." Optics and Precision Engineering 23, no. 4 (2015): 949–55. http://dx.doi.org/10.3788/ope.20152304.0949.

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28

Harmon, Jeffrey, Hideharu Mikami, Hiroshi Kanno, Takuro Ito, and Keisuke Goda. "Accurate classification of microalgae by intelligent frequency-division-multiplexed fluorescence imaging flow cytometry." OSA Continuum 3, no. 3 (2020): 430. http://dx.doi.org/10.1364/osac.387523.

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29

Haedicke, Katja, Susanna Gräfe, Frank Lehmann, and Ingrid Hilger. "Multiplexed in vivo fluorescence optical imaging of the therapeutic efficacy of photodynamic therapy." Biomaterials 34, no. 38 (2013): 10075–83. http://dx.doi.org/10.1016/j.biomaterials.2013.08.087.

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30

Theodorou, Ioannis G., Pakatip Ruenraroengsak, Daniel A. Gonzalez-Carter, et al. "Towards multiplexed near-infrared cellular imaging using gold nanostar arrays with tunable fluorescence enhancement." Nanoscale 11, no. 4 (2019): 2079–88. http://dx.doi.org/10.1039/c8nr09409h.

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31

Wegner, Kyle A., Adib Keikhosravi, Kevin W. Eliceiri, and Chad M. Vezina. "Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections." Journal of Histochemistry & Cytochemistry 65, no. 8 (2017): 479–90. http://dx.doi.org/10.1369/0022155417718541.

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The low cost and simplicity of picrosirius red (PSR) staining have driven its popularity for collagen detection in tissue sections. We extended the versatility of this method by using fluorescent imaging to detect the PSR signal and applying automated quantification tools. We also developed the first PSR protocol that is fully compatible with multiplex immunostaining, making it possible to test whether collagen structure differs across immunohistochemically labeled regions of the tissue landscape. We compared our imaging method with two gold standards in collagen imaging, linear polarized ligh

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Zhao, Ming, Yu Li, and Leilei Peng. "FPGA-based multi-channel fluorescence lifetime analysis of Fourier multiplexed frequency-sweeping lifetime imaging." Optics Express 22, no. 19 (2014): 23073. http://dx.doi.org/10.1364/oe.22.023073.

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Margineanu, Anca, Romain Laine, Sunil Kumar, et al. "Multiplexed Time Lapse Fluorescence Lifetime Readouts in an Optically Sectioning Time-Gated Imaging Microscope." Biophysical Journal 100, no. 3 (2011): 183a. http://dx.doi.org/10.1016/j.bpj.2010.12.1221.

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Matsuda, Takahiko, and Izumi Oinuma. "Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9." Molecular Biology of the Cell 30, no. 22 (2019): 2838–55. http://dx.doi.org/10.1091/mbc.e19-04-0223.

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Fluorescence imaging at single-cell resolution is a crucial approach to analyzing the spatiotemporal regulation of proteins within individual cells of complex neural networks. Here we present a nonviral strategy that enables the tagging of endogenous loci by CRISPR/Cas9-mediated genome editing combined with a nucleofection technique. The method allowed expression of fluorescently tagged proteins at endogenous levels, and we successfully achieved tagging of a presynaptic protein, synaptophysin (Syp), and a postsynaptic protein, PSD-95, in cultured postmitotic neurons. Superresolution fluorescen

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Nissler, Robert, and Sebastian Kruss. "Towards Monochiral Chemical Sensing with Near Infrared Fluorescent Carbon Nanotubes." ECS Meeting Abstracts MA2022-01, no. 9 (2022): 722. http://dx.doi.org/10.1149/ma2022-019722mtgabs.

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Semiconducting single wall carbon nanotubes (SWCNTs) fluoresce in the near infrared (NIR) and the emission wavelength depends on their chirality (n,m). Interactions with the environment affect the fluorescence and can be tailored by functionalizing SWCNTs with biopolymers such as DNA, which is the basis for fluorescent biosensors. So far, such biosensors were mainly assembled from mixtures of SWCNT chiralities with large spectral overlap, which affects sensitivity as well as selectivity and prevents multiplexed sensing. The main challenge to gain chirality pure sensors has been to combine appr

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CHAI, JINGWEN, and QING SONG. "MULTISPECTRAL CONCURRENT DETECTION OF MULTIPLE PROTEINS." Nano LIFE 02, no. 03 (2012): 1241004. http://dx.doi.org/10.1142/s1793984412410048.

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Proteins constitutively function within networks. Concurrent detection of multiple proteins is crucial to clinical diagnoses and multidimensional drug profiling. Fluorescence microscopy is capable of multicolor imaging, and has the capability to quantify essentially any physiological changes that occur at the single-cell level and in the context of live single cells, and thus provides an alternative to flow cytometry for multiplexed live single-cell assay. The staining of cells with multiple labels is still a technical challenge while multiplexed assays are complicated by spectral emission ove

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Paulson, Bjorn, Saeed Bohlooli Darian, Youngkyu Kim, et al. "Spectral Multiplexing of Fluorescent Endoscopy for Simultaneous Imaging with Multiple Fluorophores and Multiple Fields of View." Biosensors 13, no. 1 (2022): 33. http://dx.doi.org/10.3390/bios13010033.

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Complex clinical procedures and small-animal research procedures can benefit from dual-site imaging provided by multiple endoscopic devices. Here, an endoscopic system is proposed which enables multiple fluorescence microendoscopes to be spectrally multiplexed on a single microscope base, enabling light sources and optical relays to be shared between endoscopes. The presented system is characterized for resolution using USAF-1951 resolution test charts and for modulation transfer function using the slanted edge method. Imaging is demonstrated both directly and with microendoscopes attached. Im

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Xu, Jian, Tianyue Zhang, Shenyu Yang, et al. "Super-Resolution Optical Imaging: Plasmonic Nanoprobes for Multiplexed Fluorescence-Free Super-Resolution Imaging (Advanced Optical Materials 20/2018)." Advanced Optical Materials 6, no. 20 (2018): 1870077. http://dx.doi.org/10.1002/adom.201870077.

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Pham, Thai, Renjie Liao, Joshua Labaer, and Jia Guo. "Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide." Molecules 26, no. 8 (2021): 2206. http://dx.doi.org/10.3390/molecules26082206.

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Understanding the composition, function and regulation of complex cellular systems requires tools that quantify the expression of multiple proteins at their native cellular context. Here, we report a highly sensitive and accurate protein in situ profiling approach using off-the-shelf antibodies and cleavable fluorescent tyramide (CFT). In each cycle of this method, protein targets are stained with horseradish peroxidase (HRP) conjugated antibodies and CFT. Subsequently, the fluorophores are efficiently cleaved by mild chemical reagents, which simultaneously deactivate HRP. Through reiterative

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Mooney, Nancie. "Abstract 5631: Expanding tools for multiplex mRNA imaging in spatialomics through the ViewRNA tissue assay kits." Cancer Research 83, no. 7_Supplement (2023): 5631. http://dx.doi.org/10.1158/1538-7445.am2023-5631.

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Abstract Spatialomics is a rapidly growing field as it allows researchers to gain a deeper understanding of transcriptomes and corresponding protein expression profiles in cells within complex tissue microenvironments. One critical technology for spatialomics is in-situ hybridization (ISH) technology, which enables direct visualization and quantitation of nucleic acid in cells with single molecule resolution. The Invitrogen ViewRNA ISH assays incorporate branched DNA (bDNA) technology provides tools for interrogating multiple RNA transcripts at the same time, paving the way for improved spatia

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McMahon, Nathan P., Jocelyn A. Jones, Ashley N. Anderson, Matthew S. Dietz, Melissa H. Wong, and Summer L. Gibbs. "Flexible Cyclic Immunofluorescence (cyCIF) Using Oligonucleotide Barcoded Antibodies." Cancers 15, no. 3 (2023): 827. http://dx.doi.org/10.3390/cancers15030827.

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Advances in our understanding of the complex, multifaceted interactions between tumor epithelia, immune infiltrate, and tumor microenvironmental cells have been driven by highly multiplexed imaging technologies. These techniques are capable of labeling many more biomarkers than conventional immunostaining methods. However, multiplexed imaging techniques suffer from low detection sensitivity, cell loss—particularly in fragile samples—, and challenges with antibody labeling. Herein, we developed and optimized an oligonucleotide antibody barcoding strategy for cyclic immunofluorescence (cyCIF) th

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Li, Qingbo, and Edward S. Yeung. "Evaluation of the Potential of a Charge-Injection Device for DNA Sequencing by Multiplexed Capillary Electrophoresis." Applied Spectroscopy 49, no. 6 (1995): 825–33. http://dx.doi.org/10.1366/0003702953964598.

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Despite the rapid growth in the use of imaging detectors in spectroscopy, the charge-injection device (CID) has unique features that have not been fully exploited. The advantages of the CID as a two-dimensional array detector for laser-induced fluorescence detection in highly multiplexed capillary electrophoresis are evaluated. In such a system, the CID maintains both high sensitivity and high sampling rate, which are usually difficult to achieve simultaneously with other array detectors. Applying the electronic windowing function significantly improves the scan rate and greatly reduces the vo

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Chiodi, Elisa, George G. Daaboul, Allison M. Marn, and M. Selim Ünlü. "Multiplexed Affinity Measurements of Extracellular Vesicles Binding Kinetics." Sensors 21, no. 8 (2021): 2634. http://dx.doi.org/10.3390/s21082634.

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Extracellular vesicles (EVs) have attracted significant attention as impactful diagnostic biomarkers, since their properties are closely related to specific clinical conditions. However, designing experiments that involve EVs phenotyping is usually highly challenging and time-consuming, due to laborious optimization steps that require very long or even overnight incubation durations. In this work, we demonstrate label-free, real-time detection, and phenotyping of extracellular vesicles binding to a multiplexed surface. With the ability for label-free kinetic binding measurements using the Inte

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Xiao, Lu, Renjie Liao, and Jia Guo. "Highly Multiplexed Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization." Molecules 25, no. 21 (2020): 4900. http://dx.doi.org/10.3390/molecules25214900.

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The ability to comprehensively profile nucleic acids in individual cells in their natural spatial contexts is essential to advance our understanding of biology and medicine. Here, we report a novel method for spatial transcriptomics and genomics analysis. In this method, every nucleic acid molecule is detected as a fluorescent spot at its natural cellular location throughout the cycles of consecutive fluorescence in situ hybridization (C-FISH). In each C-FISH cycle, fluorescent oligonucleotide probes hybridize to the probes applied in the previous cycle, and also introduce the binding sites fo

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Tu, Siyu, David Rioux, Josée Perreault, Danny Brouard, and Michel Meunier. "Fluorescence and Scattering Dual-Mode Multiplexed Imaging with Gold–Silver Alloy Core Silica Shell Nanoparticles." Journal of Physical Chemistry C 121, no. 16 (2017): 8944–51. http://dx.doi.org/10.1021/acs.jpcc.6b11954.

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Braselmann, Esther, and Nadia Sarfraz. "Abstract 1289: Multiplexed illumination of RNAs in live mammalian cells by fluorescence lifetime imaging microscopy." Journal of Biological Chemistry 299, no. 3 (2023): S70. http://dx.doi.org/10.1016/j.jbc.2023.103200.

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47

Afsari, Hamid Samareh, Marcelina Cardoso Dos Santos, Stina Lindén, et al. "Time-gated FRET nanoassemblies for rapid and sensitive intra- and extracellular fluorescence imaging." Science Advances 2, no. 6 (2016): e1600265. http://dx.doi.org/10.1126/sciadv.1600265.

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Time-gated Förster resonance energy transfer (FRET) using the unique material combination of long-lifetime terbium complexes (Tb) and semiconductor quantum dots (QDs) provides many advantages for highly sensitive and multiplexed biosensing. Although time-gated detection can efficiently suppress sample autofluorescence and background fluorescence from directly excited FRET acceptors, Tb-to-QD FRET has rarely been exploited for biomolecular imaging. We demonstrate Tb-to-QD time-gated FRET nanoassemblies that can be applied for intra- and extracellular imaging. Immunostaining of different epitope

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Hanley, Quentin S., Peter J. Verveer, and Thomas M. Jovin. "Optical Sectioning Fluorescence Spectroscopy in a Programmable Array Microscope." Applied Spectroscopy 52, no. 6 (1998): 783–89. http://dx.doi.org/10.1366/0003702981944364.

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We report the use of a programmable array microscope (PAM) for the acquisition of spectrally resolved and high-throughput optical sections. The microscope is based on the use of a spatial light modulator for defining patterns of excitation and/or detection of fluorescence. For obtaining optically sectioned spectral images, the entrance slit of an imaging spectrograph and a line illumination pattern defined with a spatial light modulator are placed in conjugate optical positions. Compared to wide-field illumination, optical sectioning led to greater than 3× improvement in the rejection of out-o

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Gómez-García, Pablo A., Erik T. Garbacik, Jason J. Otterstrom, Maria F. Garcia-Parajo, and Melike Lakadamyali. "Excitation-multiplexed multicolor superresolution imaging with fm-STORM and fm-DNA-PAINT." Proceedings of the National Academy of Sciences 115, no. 51 (2018): 12991–96. http://dx.doi.org/10.1073/pnas.1804725115.

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Abstract:

Recent advancements in single-molecule-based superresolution microscopy have made it possible to visualize biological structures with unprecedented spatial resolution. Determining the spatial coorganization of these structures within cells under physiological and pathological conditions is an important biological goal. This goal has been stymied by the current limitations of carrying out superresolution microscopy in multiple colors. Here, we develop an approach for simultaneous multicolor superresolution imaging which relies solely on fluorophore excitation, rather than fluorescence emission

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Cooper, Justin T., and Joel M. Harris. "Spatially Multiplexed Imaging: Fluorescence Correlation Spectroscopy for Efficient Measurement of Molecular Diffusion at Solid–Liquid Interfaces." Applied Spectroscopy 70, no. 4 (2016): 695–701. http://dx.doi.org/10.1177/0003702816631312.

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