Relevant bibliographies by topics / Multivalent interaction

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Multivalent interaction'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Multivalent interaction.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Multivalent interaction"

1

Errington, Wesley J., Bence Bruncsics, and Casim A. Sarkar. "Mechanisms of noncanonical binding dynamics in multivalent protein–protein interactions." Proceedings of the National Academy of Sciences 116, no. 51 (November 27, 2019): 25659–67. http://dx.doi.org/10.1073/pnas.1902909116.

Full text
Abstract:
Protein multivalency can provide increased affinity and specificity relative to monovalent counterparts, but these emergent biochemical properties and their mechanistic underpinnings are difficult to predict as a function of the biophysical properties of the multivalent binding partners. Here, we present a mathematical model that accurately simulates binding kinetics and equilibria of multivalent protein–protein interactions as a function of the kinetics of monomer–monomer binding, the structure and topology of the multidomain interacting partners, and the valency of each partner. These properties are all experimentally or computationally estimated a priori, including approximating topology with a worm-like chain model applicable to a variety of structurally disparate systems, thus making the model predictive without parameter fitting. We conceptualize multivalent binding as a protein–protein interaction network: ligand and receptor valencies determine the number of interacting species in the network, with monomer kinetics and structural properties dictating the dynamics of each species. As predicted by the model and validated by surface plasmon resonance experiments, multivalent interactions can generate several noncanonical macroscopic binding dynamics, including a transient burst of high-energy configurations during association, biphasic equilibria resulting from interligand competition at high concentrations, and multiexponential dissociation arising from differential lifetimes of distinct network species. The transient burst was only uncovered when extending our analysis to trivalent interactions due to the significantly larger network, and we were able to predictably tune burst magnitude by altering linker rigidity. This study elucidates mechanisms of multivalent binding and establishes a framework for model-guided analysis and engineering of such interactions.
APA, Harvard, Vancouver, ISO, and other styles
2

Kauscher, Ulrike, and Bart Jan Ravoo. "Mannose-decorated cyclodextrin vesicles: The interplay of multivalency and surface density in lectin–carbohydrate recognition." Beilstein Journal of Organic Chemistry 8 (September 17, 2012): 1543–51. http://dx.doi.org/10.3762/bjoc.8.175.

Full text
Abstract:
Cyclodextrin vesicles are versatile models for biological cell membranes since they provide a bilayer membrane that can easily be modified by host–guest interactions with functional guest molecules. In this article, we investigate the multivalent interaction of the lectin concanavalin A (ConA) with cyclodextrin vesicles decorated with mannose–adamantane conjugates with one, two or three adamantane units as well as one or two mannose units. The carbohydrate–lectin interaction in this artificial, self-assembled glycocalyx was monitored in an agglutination assay by the increase of optical density at 400 nm. It was found that there is a close relation between the carbohydrate density at the cyclodextrin vesicle surface and the multivalent interaction with ConA, and the most efficient interaction (i.e., fastest agglutination at lowest concentration) was observed for mannose–adamantane conjugates, in which both the cyclodextrin–adamantane and the lectin–mannose interaction is inherently multivalent.
APA, Harvard, Vancouver, ISO, and other styles
3

Zhulina, E. B., O. V. Borisov, and T. M. Birshtein. "Polyelectrolyte Brush Interaction with Multivalent Ions." Macromolecules 32, no. 24 (November 1999): 8189–96. http://dx.doi.org/10.1021/ma981811e.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Schamel, Wolfgang W. A., Ignacio Arechaga, Ruth M. Risueño, Hisse M. van Santen, Pilar Cabezas, Cristina Risco, José M. Valpuesta, and Balbino Alarcón. "Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response." Journal of Experimental Medicine 202, no. 4 (August 8, 2005): 493–503. http://dx.doi.org/10.1084/jem.20042155.

Full text
Abstract:
A long-standing paradox in the study of T cell antigen recognition is that of the high specificity–low affinity T cell receptor (TCR)–major histocompatibility complex peptide (MHCp) interaction. The existence of multivalent TCRs could resolve this paradox because they can simultaneously improve the avidity observed for monovalent interactions and allow for cooperative effects. We have studied the stoichiometry of the TCR by Blue Native–polyacrylamide gel electrophoresis and found that the TCR exists as a mixture of monovalent (αβγεδεζζ) and multivalent complexes with two or more ligand-binding TCRα/β subunits. The coexistence of monovalent and multivalent complexes was confirmed by electron microscopy after label fracture of intact T cells, thus ruling out any possible artifact caused by detergent solubilization. We found that although only the multivalent complexes become phosphorylated at low antigen doses, both multivalent and monovalent TCRs are phosphorylated at higher doses. Thus, the multivalent TCRs could be responsible for sensing low concentrations of antigen, whereas the monovalent TCRs could be responsible for dose-response effects at high concentrations, conditions in which the multivalent TCRs are saturated. Thus, besides resolving TCR stoichiometry, these data can explain how T cells respond to a wide range of MHCp concentrations while maintaining high sensitivity.
APA, Harvard, Vancouver, ISO, and other styles
5

Tang, Jo Sing Julia, Sophia Rosencrantz, Lucas Tepper, Sany Chea, Stefanie Klöpzig, Anne Krüger-Genge, Joachim Storsberg, and Ruben R. Rosencrantz. "Functional Glyco-Nanogels for Multivalent Interaction with Lectins." Molecules 24, no. 10 (May 15, 2019): 1865. http://dx.doi.org/10.3390/molecules24101865.

Full text
Abstract:
Interactions between glycans and proteins have tremendous impact in biomolecular interactions. They are important for cell–cell interactions, proliferation and much more. Here, we emphasize the glycan-mediated interactions between pathogens and host cells. Pseudomonas aeruginosa, responsible for a huge number of nosocomial infections, is especially the focus when it comes to glycan-derivatives as pathoblockers. We present a microwave assisted protecting group free synthesis of glycomonomers based on lactose, melibiose and fucose. The monomers were polymerized in a precipitation polymerization in the presence of NiPAm to form crosslinked glyco-nanogels. The influence of reaction parameters like crosslinker type or stabilizer amount was investigated. The gels were characterized in lectin binding studies using model lectins and showed size and composition-dependent inhibition of lectin binding. Due to multivalent presentation of glycans in the gel, the inhibition was clearly stronger than with unmodified saccharides, which was compared after determination of the glycan loading. First studies with Pseudomonas aeruginosa revealed a surprising influence on the secretion of virulence factors. Functional glycogels may be in the future potent alternatives or adjuvants for antibiotic treatment of infections based on glycan interactions between host and pathogen.
APA, Harvard, Vancouver, ISO, and other styles
6

Wang, Yanyan, Srinivas Chalagalla, Tiehai Li, Xue-long Sun, Wei Zhao, Peng G. Wang, and Xiangqun Zeng. "Multivalent interaction-based carbohydrate biosensors for signal amplification." Biosensors and Bioelectronics 26, no. 3 (November 15, 2010): 996–1001. http://dx.doi.org/10.1016/j.bios.2010.08.025.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Tõugu, V., T. Kesvatera, A. Lääne, and A. Aaviksaa. "Acetylcholinesterase as polyelectrolyte: interaction with multivalent cationic inhibitors." Biochimica et Biophysica Acta (BBA) - General Subjects 1157, no. 3 (July 1993): 199–203. http://dx.doi.org/10.1016/0304-4165(93)90065-g.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Brissonnet, Yoan, Coralie Assailly, Amélie Saumonneau, Julie Bouckaert, Mike Maillasson, Clémence Petitot, Benoit Roubinet, et al. "Multivalent Thiosialosides and Their Synergistic Interaction with Pathogenic Sialidases." Chemistry - A European Journal 25, no. 9 (January 11, 2019): 2358–65. http://dx.doi.org/10.1002/chem.201805790.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Cousin, Jonathan M., and Mary J. Cloninger. "Glycodendrimers: tools to explore multivalent galectin-1 interactions." Beilstein Journal of Organic Chemistry 11 (May 12, 2015): 739–47. http://dx.doi.org/10.3762/bjoc.11.84.

Full text
Abstract:
Four generations of lactose-functionalized polyamidoamine (PAMAM) were employed to further the understanding of multivalent galectin-1 mediated interactions. Dynamic light scattering and fluorescence microscopy were used to study the multivalent interaction of galectin-1 with the glycodendrimers in solution, and glycodendrimers were observed to organize galectin-1 into nanoparticles. In the presence of a large excess of galectin-1, glycodendrimers nucleated galectin-1 into nanoparticles that were remarkably homologous in size (400–500 nm). To understand augmentation of oncologic cellular aggregation by galectin-1, glycodendrimers were used in cell-based assays with human prostate carcinoma cells (DU145). The results revealed that glycodendrimers provided competitive binding sites for galectin-1, which diverted galectin-1 from its typical function in cellular aggregation of DU145 cells.
APA, Harvard, Vancouver, ISO, and other styles
10

Nörpel, Julia, Simone Cavadini, Andreas D. Schenk, Alexandra Graff-Meyer, Daniel Hess, Jan Seebacher, Jeffrey A. Chao, and Varun Bhaskar. "Structure of the human C9orf72-SMCR8 complex reveals a multivalent protein interaction architecture." PLOS Biology 19, no. 7 (July 23, 2021): e3001344. http://dx.doi.org/10.1371/journal.pbio.3001344.

Full text
Abstract:
A major cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) spectrum disorder is the hexanucleotide G4C2 repeat expansion in the first intron of the C9orf72 gene. Many underlying mechanisms lead to manifestation of disease that include toxic gain-of-function by repeat G4C2 RNAs, dipeptide repeat proteins, and a reduction of the C9orf72 gene product. The C9orf72 protein interacts with SMCR8 and WDR41 to form a trimeric complex and regulates multiple cellular pathways including autophagy. Here, we report the structure of the C9orf72-SMCR8 complex at 3.8 Å resolution using single-particle cryo-electron microscopy (cryo-EM). The structure reveals 2 distinct dimerization interfaces between C9orf72 and SMCR8 that involves an extensive network of interactions. Homology between C9orf72-SMCR8 and Folliculin-Folliculin Interacting Protein 2 (FLCN-FNIP2), a GTPase activating protein (GAP) complex, enabled identification of a key residue within the active site of SMCR8. Further structural analysis suggested that a coiled-coil region within the uDenn domain of SMCR8 could act as an interaction platform for other coiled-coil proteins, and its deletion reduced the interaction of the C9orf72-SMCR8 complex with FIP200 upon starvation. In summary, this study contributes toward our understanding of the biological function of the C9orf72-SMCR8 complex.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Multivalent interaction"

1

Kaftan, Öznur. "Assemblages de polysaccharides hôtes et invités en surface : synthèse et rôle des interactions multivalentes." Thesis, Grenoble, 2011. http://www.theses.fr/2011GRENV020.

Full text
Abstract:
Notre étude aborde deux points importants sur les interactions supramoléculaires dans les polymères : tout d'abord comment des polymères peuvent s'assembler sur des surfaces planes au moyen de d'interactions de type hôte/invité, puis sur les interactions entre polymères à l'échelle de la molécule unique. En particulier nous verrons comment ces interactions à courte portée influent sur l'adhésion des chaînes sur des surfaces chimiquement contrôlées. Notre choix s'est porté sur un polymère d'origine naturelle le chitosane fonctionnalisé respectivement par des B-cyclodextrines (hôte) et des adamantanes (invité) et dont les assemblages forment des gels. Dans une première partie nous montrerons la possibilité de créer des multicouches de polymère par la méthode Layer-by-Layer (LbL) à l'aide des interactions de type hôte/invité, assemblage toutefois limité par les interactions électrostatiques au sein de la structure. Dans une seconde partie nous étudions les interactions multivalentes hôte/invité entre les couches de polymères en mesurant la force d'interaction par AFM. Nous avons pu mettre en évidence les différentes contributions à la force d'interaction et montrer que les interactions hôte//invités dominent les interactions non spécifiques d'un ordre de grandeur
In this study we focused on two important points concerning supramolecular interactions in polymeric systems. First; how polymers self-assemble on planar surfaces through side-chain host-guest interactions. Second; how those polymers interact each other at the level of single chain and how the adhesion properties of polymers on the modified surfaces can be controlled with those short ranged specific interactions. For that purpose a natural polysaccharide, chitosan, was chosen as the polymeric backbone and was specifically modified with host (B-cyclodextrin) and guest (adamanatane) molecules. It is known that those modified polysaccharides interact each other through host-guest units and their supramolecular assemblies exhibiting a gel-like behavior in solution state. In the first part of the study we investigated the feasibility to use supramolecular interactions to construct functional polymer multilayers by using the Layer-by-Layer (LbL) self assembly approach. The driving force with the proposed system is host-guest interactions thus short ranged and sterically demanding as the structural fitting is necessary. Our results show that multiple host-guest interactions along the chitosan chain allow the self assembles of the modified chitosans on guest-attached surfaces. The number of layers is limited and possibly affected by the electrostatic charge of the chitosan backbone. In the second part of the study we used atomic force microscope (AFM) to probe the multivalent host-guest interactions between modified polymer layers by direct force measurement. By this technique, the main contributions to the interaction between modified chitosan layers could be identified. Adhesion properties of the modified chitosans have also been investigated. The work of adhesion is about an order of magnitude larger for those chitosan derivatives that can form host-guest complexes than for those where this is not possible
APA, Harvard, Vancouver, ISO, and other styles
2

Ritt, Marie-Claude. "Thermodynamics of interaction of macrocyclic ligands with multivalent ions and organic molecules of biological importance." Thesis, University of Surrey, 1991. http://epubs.surrey.ac.uk/843105/.

Full text
Abstract:
The first part of this work deals with the determination of the thermodynamic parameters for the complexation of lanthanide cations (La3+, Pr3+ and Nd3+) with Cryptand-221 and Cryptand-222 in acetonitrile and propylene carbonate at 298.15 K. The complexation process between these cations and these ligands in these solvents is enthalpy-controlled. The higher stability observed for these cations and these ligands in propylene carbonate with respect to acetonitrile is attributed to the increase in entropy observed for the complexation reaction in propylene carbonate. Enthalpies of solution of lanthanide and lanthanide cryptates are reported. These data are used to derive single-ion enthalpies of transfer of La3+, Pr3+ and Nd3+ from propylene carbonate to acetonitrile based on the Ph4AsPh4B convention. The results show that the cryptate conventions are not valid for the calculation of single-ion values for the transfer of tervalent lanthanide cations among dipolar aprotic media. Enthalpies of coordination of lanthanide(III) cryptates in the solid state are calculated. The second part of this study aims to investigate the properties of the synthetic macrocyclic ligands such as Cryptand-222 and 18-Crown-6 towards molecules of biological importance. Stability constants (hence free energies), enthalpies and entropies of complexation of a series of DL-amino acids with 18-Crown-6 and Cryptand-222 in methanol and ethanol, as obtained from titration calorimetry, are reported. No significant variations are found in the free energies of complexation of the different amino acids and these two ligands in these solvents as a result of an enthalpy-entropy compensation effect. This effect is for the first time shown in complexation reactions involving crown ethers and cryptands. The thermodynamic parameters of transfer of amino acids and their complexes with 18-Crown-6 and Cryptand-222 from methanol to ethanol have been calculated. Possible correlations between complexation and transfer data for the amino acids, the ligands and the amino acid-macrocyclic ligand complexes are investigated. The implications of these results to processes of biological importance are discussed. As a continuation of this study, the possibility of selectively extracting amino acids from methanol by polymeric resins containing crown ethers as anchor groups is investigated.
APA, Harvard, Vancouver, ISO, and other styles
3

Zhou, Min. "Multivalent Interactions Based on Supramolecular Self-Assembly and Peptide-Labeled Quantum Dots for Imaging GPCRs." Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/195308.

Full text
Abstract:
Multivalent interactions are common in nature, such as influenza virus infecting epithelial cells, clearance of pathogens by antibody-mediated attachment to macrophages, etc. To mimic nature, we utilized a bottom-up approach to develop various multivalent self-assembling systems based on leucine-zipper peptides. We tethered several pairs of leucine-zipper peptides to PAMAM dendrimers to form leucine-zipper dendrimers (LZDs). We conjugated Fos/Jun to the dendrimer to make D0Fos4 and D0Jun4, and studied the interactions between these LZDs and their cognate peptide target, either Jun or Fos. Our experiments showed that the D0Fos4 can non-covalently assemble four copies of Jun, and this approach can be further used for the rapid non-covalently assembling of multimeric ligands. We also pursued the multivalent target of GPCRs with a Fos/Jun assembly, and found the complex can potentially be used as a molecular switch to target GPCRs with controlled ligand activity. In a related project for bio-material design based on self-assembly of LZDs, we synthesized a different pair of LZDs, D-Ez4 and D-Kz4, and established that they can assemble at neutral pH to form helical fibrils which display higher order self-organized structures, providing a new methodology for bio-material design. In another effort for studying multivalent interactions, we conjugated three copies of the F23, mini-protein that binds the HIV-1 capsid protein, to a trimesic acid and obtained a trivalent inhibitor, Tri-F23. Tri-F23 showed enhanced binding in ELISA against gp120, but was not significantly more effective preventing HIV entry. This methodology provides a new strategy for developing multivalent inhibitors for preventing HIV-1 infection at the entry level. In a related area, we are developing imaging agents based on quantum dots that can detect GPCRs on whole cells and at the single molecule level. To this end, a new method was developed for biocompatible amphphilic polymers to coat quantum dots. This amphiphilic polymer facilitates rapid quantum dot conjugation to any ligand with a free thiol or engineered cysteine. Several GPCR targeted peptides have been utilized for imaging receptors on whole cells and as single molecules. These efforts will guide the rational design of multivalent ligands for targeting GPCRs and other cell surface proteins.
APA, Harvard, Vancouver, ISO, and other styles
4

Walkowiak, Jacek [Verfasser], Matthias [Akademischer Betreuer] Ballauff, Michael [Akademischer Betreuer] Gradzielski, Matthias [Gutachter] Ballauff, Michael [Gutachter] Gradzielski, and Alexander [Gutachter] Böker. "Interaction of proteins with multivalent polyelectrolytes / Jacek Walkowiak ; Gutachter: Matthias Ballauff, Michael Gradzielski, Alexander Böker ; Matthias Ballauff, Michael Gradzielski." Berlin : Technische Universität Berlin, 2020. http://d-nb.info/121957385X/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Laigre, Eugénie. "Conception, synthèse et étude de modules de reconnaissance multivalents pour des anticorps." Thesis, Université Grenoble Alpes (ComUE), 2018. http://www.theses.fr/2018GREAV038/document.

Full text
Abstract:
En dépit d’importants progrès dans le domaine de la thérapie anti-cancéreuse, les traitements actuels restent controversés, notamment en raison de la quantité importante d'effets secondaires induits. L'immunothérapie ciblée a récemment émergée en tant qu'alternative, afin d'améliorer les modalités de traitement des patients atteints du cancer. Malgré tout, seul un nombre limité d’approches sont aujourd’hui disponibles, et une grande partie des problèmes demeurent actuellement sans solution. C'est dans ce contexte que nous nous sommes intéressés à la conception de structures biomoléculaires innovantes et bifonctionnelles, capables de rediriger des anticorps endogènes, présents naturellement dans la circulation sanguine de l'homme, contre les tumeurs et, ce, sans immunisation préalable. Les anticorps naturels circulant étant polyspécifiques et ayant la capacité d’interagir avec des antigènes glycosylés, nous nous sommes plus particulièrement concentrés sur la conception de glycoconjugués multivalents, ligands d’anticorps endogènes. Une première partie de notre étude a consisté à synthétiser différents glycodendrimères multivalents, reposant sur des châssis peptidiques et obtenus par ligations chimiosélectives, tout en variant la nature du motif glycosylé et des plateformes, ainsi que la valence du conjugué. Puis, dans un second temps, des tests d’interaction par biopuce ont été mis en place avec une lectine modèle, la lectine Helix Pomatia Agglutinin (HPA). Des protocoles expérimentaux visant à calculer des constantes de dissociation de surface, ainsi que des IC50 ont été mis en place, permettant d’identifier de bons ligands de HPA avec des affinités de l’ordre du nanomolaire. Les tests par biopuce ont ensuite été confirmés avec d’autres méthodes d’analyses (BLI, ELLA). Finalement, afin d'identifier des architectures tri-dimensionnelles permettant une affinité optimale avec des anticorps, les tests d’interaction ont été adaptés au criblage de séra humains. Un large panel de glycoconjugués a alors été criblé par biopuce avec une vingtaine de séra, permettant la détermination de structures glycosylés prometteuses, qui pourront par la suite être utilisées dans le cadre de notre approche anti-cancéreuse
Despite significant progress in anti-cancer therapy, current treatments are still controversial due to numerous side effects. Targeted immunotherapy recently emerged as an ideal alternative to improve treatment modalities for cancer patients. However, very limited approaches are available today and major issues remain to be addressed. In this context, we are interested in the design of biomolecular structures, innovative and bifunctional, able to hijack endogenous antibodies - which are naturally present in the human blood stream - toward cancer cells without pre-immunisation. Since natural circulating antibodies are polyspecific and have the ability to interact with multiple carbohydrate antigens, we focused on the design of multivalent glycodendrimers, as ligands for endogenous antibodies. The first part of our study consisted in synthesizing several multivalent glycoconjugates, based on peptide scaffolds and obtained by chemoselective ligations. To evaluate their influence on antibodies, the nature of both the carbohydrate and the scaffold, and the valency were varied. Then, in a second part of the study, microarray assays were developed with a model lectin, the Helix Pomatia Agglutinin (HPA). Experimental procedures were designed to determine surface dissociation constant and IC50 values, leading to the identification of high affinity ligands for HPA in the nanomolar range. Microarray assays were confirmed by other analytical methods (BLI, ELLA). Finally, the assays on slides were adapted to human sera screening, in order to identify tridimensional architectures highly affine to sera antibodies. A large panel of glycoconjugates were screened by microarray with around twenty sera, leading to the determination of promising glycosylated structures, as antibody ligands. The latter could be subsequently used for our anti-cancer approach
APA, Harvard, Vancouver, ISO, and other styles
6

Curk, Tine. "Modelling multivalent interactons." Thesis, University of Cambridge, 2016. https://www.repository.cam.ac.uk/handle/1810/266916.

Full text
Abstract:
A Multivalent entity, which could represent a protein, nanoparticle, polymer, virus or a lipid bilayer, has the ability to form multiple bonds to a substrate. Hence, a multivalent interaction can be strong, even if the individual bonds are weak. However, much more interestingly, multivalency enables the design of highly specific interactions using non-specific individual bonds. We attempt to rationalise multivalent effects using simple physical models complemented with numerical simulations. Based on physiochemical characteristics of multivalent binders, we aim to predict the overall strength of interaction and its sensitivity to variation in parameters. We start with a simple model of homo-multivalency, where all bonds are equivalent. Such systems can exhibit a super-selective response, which denotes the high sensitivity of the strength of multivalent binding to the number of accessible binding sites on the target surface. We present a theoretical analysis of systems of multivalent particles and show that a certain degree of disorder is necessary for super-selective behaviour. Moreover, we formulate a set of simple design rules for multivalent interactions that yield optimal selectivity. In the second stage, we expand the model to hetero-multivalency, accounting for multiple distinct types of binding partners. We consider targeting of cells based on a density profile of different membrane receptors types and demonstrate, that speci city towards a desired receptor density profile can be obtained. Hence, cells can be reliably targeted in the absence of specific markers. Crucially, we show that for optimal selectivity, individual bonds must be weak. Finally, we add information about specific geometry and positions of binding sites on the multivalent entity. We focus on molecular imprinting; the process whereby a polymer matrix is cross-linked in the presence of template molecules. The cross-linking process endows the polymer matrix with a chemical ‘memory’, such that the target molecules can subsequently be recognised by the matrix. We show how the binding multivalency and the polymer material properties affect the efficiency and selectivity of molecular imprinting.
APA, Harvard, Vancouver, ISO, and other styles
7

Yilmaz, Gokhan. "Synthesis of glycomaterials for multivalent interactions." Thesis, University of Warwick, 2016. http://wrap.warwick.ac.uk/89300/.

Full text
Abstract:
Carbohydrates have attracted much attention to insert their biological properties into nanostructured materials due to their use for bio-mimetic purposes, their crucial role in bio-recognition processes at molecular level and their functional role in living systems. Glycopolymers, which are synthetic macromolecules with sugar moieties, exhibit a crucial role for many biological processes such as signal transmission, intercellular recognition and fertilization. The interaction between carbohydrates and lectins could be greatly enhanced by the multivalent effect of densely packed carbohydrate molecules with unique functionalities, which is known as the “glycocluster effect”. Therefore, the investigation of this specific interaction between glycopolymer and protein is very important to create more complex and biologically relevant carbohydrate mimics. Well-defined amphiphilic block glycopolymers with the same mannose content have been self-assembled in aqueous solution to form glyconanoparticles with different morphologies (spherical, worm-like micelles and vesicles). The size and shape of nanoparticles have significant effects on the binding affinities with dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DCSIGN). Moreover, the obtained glyco-micelles have a great potential for drug delivery applications. Glycopolymer-coated gold nanoparticles (glyco-AuNPs) which were synthesized with reversible addition-fragmentation chain transfer (RAFT) polymerization were combined with doxorubicin (DOX) as a model anticancer drug by creating a pHsensitive hydrazone linkage in the presence of cysteine (Cys) and a cross-linker for both chemotherapy and radiation therapy. The reversible single-chain glycopolymer folding structures in α-shape with different sugar moieties were created to investigate the influence of this folded collapse on the binding capability with different lectins. The single-chain folding structures were achieved by the host-quest interaction of β-cyclodextrin and adamantane in very high diluted aqueous solution. The binding results evidenced that these single-chain folded structures enhanced greatly the multivalent interaction. A new S-glucosyl substituted 2-oxazoline glycomonomer was synthesized via thiolene “click” chemistry and then polymerized using cationic ring-opening polymerization (CROP) technique. In order to investigate the effect of S-glucosyl substituent linker length on the cloud point and binding ability systematically, A series of well-defined glyco-copolymers with different sugar linker length to the polymer backbone was prepared. The obtained results showed that it has a significant influence on the cloud point and binding capability.
APA, Harvard, Vancouver, ISO, and other styles
8

Reiter-Scherer, Valentin D. "Multivalency in the interaction of biological polymers." Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21711.

Full text
Abstract:
Diese Dissertation konzentriert sich auf die Untersuchung multivalenter Wechselwirkungen zwischen Hämagglutinin (HA) sowie Neuraminidase (NA) zweier Stämme des Influenzavirus (H1N1 und H3N2) und dem zellulären Liganden Sialinsäure (SA) unter Verwendung von Rasterkraftmikroskopie und Einzelmolekülkraftspektroskopie (SMFS). Bindungskräfte sowie Dissoziations- und Assoziationskinetiken, zusammen mit den intermolekularen Potentiallandschaften wurden, nach bestem Wissen erstmalig, auf Einzelmolekülebene mittels SMFS quantifiziert. Zu diesem Zweck wurden mono- und multivalente SA-Liganden (SAPEGLA und dPGSA) eingesetzt. Abweichungen der experimentellen Kraftspektren vom klassischen Kramers-Bell-Evans-Modell vorhergesagten Verhalten wurden durch das Friddle-Noy-De Yoreo-Model berücksichtigt. NA beider Virusstämme zeigte trotz ähnlicher Bindungskräfte eine stabilere Bindung mit SA als HA und dissoziierte 3 – 7 mal langsamer. Es wird vermutet, dass die höhere Stabilität die geringere Oberflächendichte von NA auf der Virushülle im Vergleich zu HA ausgleicht. Die Bindungskräfte eines SAPEGLA-Clusters nehmen mit der Anzahl der Bindungen und die Dissoziationskinetik folgt dem theoretisch vorhergesagten Trend. Die Dissoziationsrate von NA ist etwa 6-mal höher ist als ihre katalytische Rate, weshalb Mehrfachbindungen zur Spaltung von SA erforderlich sind. Die Dissoziationsrate von N1 in der gleichen Größenordnung wie die von H3 und es wird vermutet, dass derartige Ähnlichkeiten die Übertragbarkeit des Virus begünstigen. Darüber hinaus wird gezeigt, dass die thermische Stabilität von HA-dPGSA höher ist als von HA-SAPEGLA und im Bereich von 3 - 4 Einzelbindungen liegt, was für NA-dPGSA nicht beobachtet werden konnte. Daher bindet dPGSA spezifisch und kooperativ multivalent an HA. Kompetitive Bindungstests zeigen, dass SMFS zum Screening von antiviralen Inhibitoren verwendet werden und Zugang zu deren Design auf Einzelmolekülebene liefern könnte.
This thesis focuses on studying multivalent interactions between influenza virus hemagglutinin (HA) as well as neuraminidase (NA) of two viral strains (H1N1 and H3N2) and the cellular ligand sialic acid (SA) by using scanning force microscopy and single molecule force spectroscopy (SMFS). Unbinding forces as well as dissociation and association kinetics together with the free energy landscapes were, to the best knowledge for the first time, individually quantified on the single molecule level using SMFS. To this extent, designed synthetic monovalent (SAPEGLA) and multivalent (dPGSA) SA displaying ligands were employed. Surprisingly, the experimental force spectra did not show the log-linear trend predicted by the classical Kramers-Bell-Evans model, but rather follow the more recent Friddle-Noy-De Yoreo model. NA of both viral strains forms a more stable bond with SA than HA, and dissociates 3 to 7 times slower. It is reasoned that the higher stability compensates for the lesser amount of NA compared to HA that is typically found on the viral envelope. The unbinding forces of the cluster of SAPEGLA increased gradually with the number of bonds in the cluster and the dissociation kinetics follow the theoretically predicted trend. The dissociation rate of NA was found to be about 6 times higher than its catalytic rate, indicating that multiple bonds are needed for cleavage of SA. The dissociation rate of N1 is on the same order as that of H3, suggesting that these similarities between the two strains favor transmissibility. The thermal stability of the HA-dPGSA bond is higher than the HA-SAPEGLA reaching that of three to four single bonds, proving specificity and cooperativity. Such an enhancement could not be observed for the binding of NA. This thesis also shows that SMFS could be used as a tool to screen antiviral inhibitors in competitive binding assays, which may contribute insight into the design of antiviral inhibitors on the single molecule level.
APA, Harvard, Vancouver, ISO, and other styles
9

Lam, Polo Chun Hung. "Experimental and Computational Studies in Bioorganic and Synthetic Organic Chemistry." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/40013.

Full text
Abstract:
Cationâ Ï interaction is an important determinant in protein structure and function. Among the three proteinogenic aromatic amino acids, tryptophan (Trp) is the strongest cationâ Ï donor. We reported the asymmetric syntheses of tryptophan regioisomers in which the amino acid side chain is attached at different position of the indole moiety. These new tryptophan regioisomers can effect a different mode of cationâ Ï interaction. In nature, dramatic increases in binding affinity can be achieved through multivalent binding. Following a fragmentation-dimerization approach, we synthesized Taxol-based dimer in which the baccatin III core of Taxol is coupled with flexible PEG linker. However, microtubule assembly assay suggested that these new dimers are not capable of effecting bivalent binding to the Taxol binding sites in microtubules. Memory of chirality (MOC) is an emerging theme in asymmetric synthesis in which the dynamic chirality of the reactive intermediate "memorizes" the static chirality of the reactant. Using dynamic 1D and 2D NMR and density functional theory (DFT) methods, we studied the MOC effect of 1,4-benzodiazepin-2-ones. Reconstruction of the reaction pathway using DFT calculations supported our proposed contra steric, retention of configuration mechanism.
Ph. D.
APA, Harvard, Vancouver, ISO, and other styles
10

Zanini, Diana. "Quantitative multivalent carbohydrate-protein interactions from novel glycodendrimers." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0016/NQ28389.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Multivalent interaction"

1

Ng, David. Wiring the brain with Neto1: A multivalent NMDA receptor interacting CUB domain protein with essential roles in axon guidance, synaptic plasticity, and hippocampal-dependant spatial learning and memory. 2006.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Jones, Alisha Lola. Flaming? Oxford University Press, 2020. http://dx.doi.org/10.1093/oso/9780190065416.001.0001.

Full text
Abstract:
Flaming?: The Peculiar Theopolitics of Fire and Desire in Black Male Gospel Performance examines the rituals and social interactions of African American men who use gospel music-making as a means of worshiping God and performing gendered identities. Prompted by the popular term “flaming” that is used to identify over-the-top or peculiar performance of identity, Flaming? argues that these men wield and interweave a variety of multivalent aural-visual cues, including vocal style, gesture, attire, and homiletics, to position themselves along a spectrum of gender identities. These multisensory enactments empower artists (i.e., “peculiar people”) to demonstrate modes of “competence” that affirm their fitness to minister through speech and song. Through a progression of transcongregational case studies, Flaming? observes the ways in which African American men traverse tightly knit social networks to negotiate their identities through and beyond the worship experience. Coded and “read” as either hypermasculine, queer, or sexually ambiguous, peculiar gospel performances are often a locus of nuanced protest, facilitating a critique of heteronormative theology while affording African American men opportunities for greater visibility and access to leadership. Same-sex relationships among men constitute an open secret that is carefully guarded by those who elect to remain silent in the face of traditional theology, but musically performed by those compelled to worship “in Spirit and in truth.” This book thus examines the performative mechanisms through which black men acquire an aura of sexual ambiguity, exhibit an ostensible absence of sexual preference, and thereby gain social and ritual prestige in gospel music circles.
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Multivalent interaction"

1

Huang, Zehuan, and Xi Zhang. "Cucurbit[n ]uril-Mediated Multiple Interactions." In Multivalency, 143–52. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781119143505.ch6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Chuang, Hong-Yang. "RM2 Antigen: Structural Characterization and Determination of K D,Surf for Multivalent Carbohydrate–Protein Interaction." In Springer Theses, 57–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46848-7_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Monteiro, João T., and Bernd Lepenies. "Multivalent Lectin-Glycan Interactions in the Immune System." In Multivalency, 325–44. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781119143505.ch13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Curk, Tine, Jure Dobnikar, and Daan Frenkel. "Design Principles for Super Selectivity using Multivalent Interactions." In Multivalency, 75–101. Chichester, UK: John Wiley & Sons, Ltd, 2017. http://dx.doi.org/10.1002/9781119143505.ch3.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Akiyama, Ryo. "Theoretical Studies of Strong Attractive Interaction Between Macro-anions Mediated by Multivalent Metal Cations and Related Association Behavior: Effective Interaction Between ATP-Binding Proteins Can Be Regulated by Hydrolysis." In The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery, 53–67. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8459-1_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Brewer, C. Fred. "Lectin Cross-Linking Interactions with Multivalent Carbohydrates." In The Molecular Immunology of Complex Carbohydrates —2, 17–25. Boston, MA: Springer US, 2001. http://dx.doi.org/10.1007/978-1-4615-1267-7_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Lahmann, Martina. "Architectures of Multivalent Glycomimetics for Probing Carbohydrate–Lectin Interactions." In Glycoscience and Microbial Adhesion, 183–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/128_2008_30.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Biloria, Nimish, and Dimitra Dritsa. "Real-Time Interactive Multimodal Systems for Physiological and Emotional Wellbeing." In Data-driven Multivalence in the Built Environment, 181–203. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-12180-8_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Pieters, Roland J. "Enhanced Inhibition of Protein Carbohydrate Interactions by Dendritic Multivalent Glycoligands." In ACS Symposium Series, 91–103. Washington, DC: American Chemical Society, 2011. http://dx.doi.org/10.1021/bk-2011-1091.ch006.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Lindhorst, Thisbe K. "Artificial Multivalent Sugar Ligands to Understand and Manipulate Carbohydrate-Protein Interactions." In Host-Guest Chemistry, 201–35. Berlin, Heidelberg: Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/3-540-45010-6_7.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Multivalent interaction"

1

Tan, Qiyan, Weichuan Guo, Gutian Zhao, Yajing Kan, Yinghua Qiu, and Yunfei Chen. "Charge Inversion of Mica Surface in Multivalent Electrolytes." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-62356.

Full text
Abstract:
Interaction between solid surfaces in aqueous electrolyte solutions is of great importance to many diverse domains, such as bioMEMS, nanofluidics, colloid science, polymer physics, and molecular biophysics. Several counterintuitive phenomena occur at high concentrations of multivalent ions. In this article, charge reversion, the sign reversal of the effective surface charge in the presence of multivalent counterions, is directly observed through the force between two mica surfaces in aqueous solutions of trivalent cations by surface forces measurements. The effective surface potential functioned with bulk concentration is calculated from an analytical model based on ion correlations. The obtained force profiles can be described by the analytical model when ion correlations effects are taken into account, while Poisson-Boltzmann theory fails. It reveals that ion correlations are important for screening charged surface in the presence of multivalent counterions.
APA, Harvard, Vancouver, ISO, and other styles
2

Liu, Yaling, Jifu Tan, and Samar Shah. "A Hybrid Model for Nanoparticle Targeted Delivery in Blood Flow." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13253.

Full text
Abstract:
Nanoparticle targeted delivery in vascular system involves the interplay of transport, hydrodynamic force, and multivalent interactions with targeted biosurfaces. Current theoretical studies in nanoparticle therapeutic delivery are limited to nanoparticle suspensions in a Newtonian fluid without blood cells [1–3]. However, blood is a complex biological fluid made of components such as red blood cells (RBC), monocytes, platelets, proteins, etc. The existence of blood cells in the core region of blood streams might change the nanoparticle dispersion and binding through cell-nanoparticle interaction. It is thus important to understand how blood cells influence nanoparticles motion and binding.
APA, Harvard, Vancouver, ISO, and other styles
3

Tung, Yen-Ting, Ruei-Ning Jhang, Yi-Ling Lin, and Gou-Jen Wang. "Determine the Binding Epitope of the Low Affinity Interaction between Dengue Virus and CLEC5A by a Multivalent-Interaction-Reinforcing Sensor Surface." In 2018 IEEE 12th International Conference on Nano/Molecular Medicine and Engineering (NANOMED). IEEE, 2018. http://dx.doi.org/10.1109/nanomed.2018.8641682.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Kwak, Hyung Tae, Ali A. Yousef, and Salah Al-Saleh. "New Insights on the Role of Multivalent Ions in Water-Carbonate Rock Interactions." In SPE Improved Oil Recovery Symposium. Society of Petroleum Engineers, 2014. http://dx.doi.org/10.2118/169112-ms.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Sporn, L. A., V. J. Marder, and D. D. Wagner. "VON WILLEBRAND FACTOR RELEASED FROM WEIBEL-PALADE BODIES BINDS MORE AVIDLY TO EXTRACELLULAR MATRIX THAN THAT SECRETED CONSTITUTIVELY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642835.

Full text
Abstract:
Large multimers of von Willebrand factor (vWf) are released from the Weibel-Palade bodies (WPB) of cultured endothelial cells following treatment with a secretagogue, whereas predominantly dimeric forms are secreted constitutively. These two pools of vWf were used to compare binding of the various multimeric forms of vWf to the extracellular matrix (ECM), the in vitro model of the basement membrane. The released multimers and an equal number of subunits of constitutively secreted vWf were placed, for 72 hours, on cultures of human foreskin fibroblasts (HFF) grown on glass coverslips, then fixed and stained by fluorescence using anti-vWf antiserum. Constitutively secreted vWf produced only a trace of matrix decoration, whereas the released large multimers bound more extensively. In order to determine if increased binding of released vWf was due to the presence of another component in the releasate, releasate from which vWf was adsorbed was combined with constitutively secreted vWf, and this mixture was overlaid onto HFF. The presence of the adsorbed releasate did not promote binding of constitutively secreted vWf. Therefore, it appears that the enhanced binding observed was due to the large multimeric size of vWf stored in the WPB. To further substantiate this, iodinated plasma vWf which was presumably constitutively secreted from endothelial cells was overlaid on.to HFF for 72 hours, labeled vWf was removed, and cells were washed extensively and lysed. Samples of iodinated plasma vWf (starting material) and cell lysates were e1ectrophoresed, non-reduced on an agarose gel. Densitometric scans of starting material and of bound vWf revealed that the large multimeric forms bound preferentially. It appears that multivalency is likely an important property in vWf interaction with the ECM, just as has been shown for vWf interaction with platelets. The pool of vWf contained within the WPBs, therefore, is not only especially suited for platelet interaction, but also for interaction with the ECM.
APA, Harvard, Vancouver, ISO, and other styles
6

Lindon, J. N., L. Kushner, E. Shiba, and E. W. Salzman. "PLATELET ADHESION ON SYNTHETIC SURFACES PRETREATED WITH DILUTED PLASMA IS DETERMINED BY THE SURFACE CONCENTRATION OF "NATIVE" FIBRINOGEN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643551.

Full text
Abstract:
Platelet adhesion and activation on synthetic surfaces are thought to require the prior adsorption of fibrinogen. We have reported that platelet activation by polyalkyl methacrylates (measured in bead columns exposed to flowing blood) is better correlated with the concentration of conformationally unaltered, "native" (recognizable by radiolabeled antifibrinogen antibodies) bound fibrinogen than with total bound fibrinogen (measured with radiolabeled fibrinogen) following exposure of the surfaces to purified fibrinogen in solution or to diluted blood plasma (Blood 68:355, 1986). We now report that adhesion of washed platelets to polybutyl methacrylate (PBMA; approx. 20% retention in bead columns), was unaffected by preincubation of the surface with whole plasma but was increased significantly by precoating with diluted plasma, with maximum retention (approx. 65%) occurring with plasma diluted 3000-fold. Upon exposure of PBMA to various plasma dilutions the surface concentration of antibody-detectable fibrinogen, but. not of total surface-bound fibrinogen, was correlated with the activation of washed platelets by such pretreated surfaces, With maximal platelet reactivity occurring in columns precoated with the plasma dilution (1:3000) that produced the highest concentration of "native" surface-bound fibrinogen. The plasma dilution that gave maximum total fibrinogen adsorption (100-fold dilution) was not correlated with the concentration of antibody-detectable fibrinogen. Fab fragments of polyclonal anti-fibrinogen antibodies totally prevented platelet activation by PBMA surfaces precoated with diluted plasma. It appears that participation of surface-bound fibrinogen in platelet activation on some artificial surfaces requires that fibrinogen be adsorbed in a conformationally "native" state, presumably thereby permitting multivalent, cooperative interactions with GP IIb/IIIa receptors on platelets.
APA, Harvard, Vancouver, ISO, and other styles
7

Cevheri, Necmettin, and Minami Yoda. "Evanescent-Wave Particle Velocimetry Studies of Electrokinetically Driven Flows: Divalent Counterion Effects." In ASME 2012 Third International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/mnhmt2012-75274.

Full text
Abstract:
Characterizing the mainly incompressible and laminar flows of aqueous electrolyte solutions through channels with an overall dimension of O(1–100 μm) is of interest in a variety of microfluidics applications. Solid surfaces such as the channel wall become (usually negatively) charged due to direct ionization or dissociation of surface groups, where the charge is typically characterized by the wall zeta-potential ζw. The surface in turn attracts mobile counterions from the fluid to form a (usually positively) charged screening, or electric double, layer (EDL). An external electric field can therefore be used to “pump” fluids through microfluidic Labs-on-a-Chip (LOC) by driving the charged fluid in the EDL. The resulting electroosmotic flow (EOF) is uniform outside the EDL, which has a thickness less than 50 nm in most cases. This uniform flow results in a more favorable scaling of the volume flowrate with channel diameter for microchannels, and also has less convective dispersion than shear flows. Electroosmotic flow is, however, very sensitive to changes in ζw. Various studies have shown, for example, that adding multivalent counterions to a monovalent electrolyte solution can greatly change ζw through both electrostatic and chemical interactions, even leading to “charge inversion” where the zeta-potential changes its sign. Evanescent-wave particle velocimetry, which tracks the motion of colloidal fluorescent tracer particles illuminated by evanescent waves within ∼400 nm of the wall, was therefore used to study the flow of various aqueous monovalent electrolyte solutions with small amounts of divalent cations such as Mg++ driven by an electric field through channels with a minimum dimension of ∼30 μm. The technique measures both the velocity components parallel to the wall and the steady-state distribution of these near-wall tracers. In these experiments, the tracers are convected parallel to the wall by both the EOF and directly by the applied electric field via electrophoresis because the surfaces of the particles also become negatively charged when suspended in the electrolyte solution. The electrophoretic contribution to the measured particle velocity was determined by measuring the particle zeta-potential with light scattering, and subtracted from the particle velocity to determine the actual EOF velocity.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Multivalent interaction' for particular source types:
Journal articles Dissertations / Theses
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography