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Journal articles on the topic 'Murashige and Skoog Medium'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Arfa, Nadia Fakhriyati, Endang Nurcahyani, Zulkifli Zulkifli, and Tundjung Tripeni Handayani. "Nepenthes mirabilis (Lour.) Druce Planlet at a Various Levels of Murashige & Skoog Medium Density In Vitro." Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati 6, no. 2 (2019): 18–22. http://dx.doi.org/10.23960/jbekh.v6i2.25.

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This study aims to determine the variation of the stomata index of the Kantong Semar (Nepenthes mirabilis) planlet at a various medium density of the Murashige and Skoog. This study used a Completely Randomized Design using one factor (medium density of the Murashige and Skoog). We used 5 levels of medium density, i.e.: 1/16 MS, 1/8 MS, 1/4 MS, 1/2 MS, and MS. Homogeneity test used Levene’s test of 5% significance level, then analysis of variance is carried out at 5% significance level and followed by Tukey test at 5% significance level. The results showed that the lower the level of Murashige

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2

Flinn, Barry S., David T. Webb, and Wanda Georgis. "In vitro control of caulogenesis by growth regulators and media components in embryonic explants of eastern white pine (Pinus strobus)." Canadian Journal of Botany 64, no. 9 (1986): 1948–56. http://dx.doi.org/10.1139/b86-258.

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Horizontally oriented embryos of Pinus strobus (L.) produced shoots on Schenk and Hildebrandt medium containing cytokinin. Shoots developed primarily from cotyledons in contact with the medium. Seed pretreatments at 5 or 27 °C did not affect caulogenesis. N6-Benzyladenine (BA) and N6-(Δ2-isopentenyl)adenine (2iP) both induced caulogenesis, with BA being 10–20 times more potent than 2iP. High BA levels caused callus formation. BA exposures from 1 to 8 weeks were equally caulogenic with horizontal explants, but exposures longer than 2 weeks led to increased variability and callus formation. A 1-

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3

Srilestari, Rina, and Suwardi Suwardi. "INDUCTION OF ABACA BANANA ROOTS BY IN VITRO USING KINDS OF MEDIA AND THIAMIN." Agrivet 26, no. 1 (2021): 1. http://dx.doi.org/10.31315/agrivet.v26i1.4304.

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Abaca is a type a banana with high economic value with it stem fiber used in textile and paper industries. As a superior commodity, its number is relatively limited, with the need of a largeplanting area to meet the high market demand. The aim of the research was to observe the abaca banana explants response to various media and Thiamin. The experiment was done at Biotechnology laboratory, UPN “Veteran” Yogyakarta. Treatments were arranged in completely randomized design with 2 factor. The first factor is the growing media: Murashige & Skoog, a half Murashige & Skoog media, Vacint &amp

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4

Das, P. "Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis." Horticultural Science 37, No. 2 (2010): 70–78. http://dx.doi.org/10.17221/57/2009-hortsci.

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Protocols were developed for propagation of Rosa hybrida cv. Landora and Mussaenda erythrophylla cv. Rosea via somatic embryogenesis by manipulating growth regulators and culture conditions. Calli were induced from young leaf explants of Rosa hybrida cv. Landora and Mussaenda erythrophylla cv. Rosea on Murashige, Skoog medium supplemented with 6-benzylaminopurine or kinetin along with indole-3-acetic acid or 2,4-dichloroacetic acid within four weeks of culture. The calli were subcultured either in the same medium or in a modified medium for induction of embryogenic callus. Embryogenic calli in

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Mpika, Joseph, Chanelle Bertille Mvoumbi Tete, Chrichina Mbon Nguekou, Blaise Pascal Ngondo, Laurine Valerie Mboutol-Mandavo, and Attibayeba a. "ORGANOGENESE IN VITRO DES FRAGMENTS DE TUBERCULES DE POMME DE TERRE (SOLANUM TUBEROSUM L. VAR. AIDA) CULTIVES DANS LE DEPARTEMENT DES PLATEAUX (REPUBLIQUE DU CONGO)." International Journal of Advanced Research 10, no. 08 (2022): 1065–73. http://dx.doi.org/10.21474/ijar01/15270.

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The production of potatoes rich in starch and essential nutrients does not cover the needs of Congolese consumers. This is due to strong genetic erosion and the absence of high-performance local varieties as well as the unavailability of seeds for new plantations. The seed produced by traditional techniques does not meet the demand of producers. This study aims to produce seed potatoes by the technique of in vitro culture. Tubers disinfected with 10% sodium hypochlorite are kept at 4Ã‚Â°C for 20 days to produce microtubers by bud burst others are placed in heat therapy conditions to produce mi

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6

Shahriyar, Sayeed, Soleh Akram, Koushik Khan, Md Faruk Miya, and Md Abdur Rauf Sarkar. "In vitro plant regeneration of potato (Solanum tuberosum L.) at the rate of different hormonal concentration." Asian Journal of Medical and Biological Research 1, no. 2 (2015): 297–303. http://dx.doi.org/10.3329/ajmbr.v1i2.25625.

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One of the goals of the experiment is to standardization of HgCl2 treatment for explants sterilization. The objectives also include developing a reproducible cost effective protocol for large scale production of Solanum tuberosum of Cardinal variety plantlets from selectively better clones through plant in vitro propagation methods. Selection of growth regulators for proper multiple shoots regeneration, elongation and root induction. To produce genetically uniform plantlets within a short time capable surviving in natural condition raised in in vitro environment. Shoot tip and nodal segment ex

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7

Abdelazeez, Walla Abdelmaksood, Landysh Zavdetovna Khusnetdinova, and Olga Arnoldovna Timofeeva. "Future outlook of plant growth regulators application influencing callus induction from different type explant Hyoscyamus muticus L. in vitro." Samara Journal of Science 7, no. 3 (2018): 10–13. http://dx.doi.org/10.17816/snv201873101.

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The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of Hyoscyamus muticus L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of Hyoscyamus muticus L. in vitro was

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8

GALATOWITSCH, M. W., and G. A. SMITH. "REGENERATION FROM UNFERTILIZED OVULE CALLUS OF SUGARBEET (Beta vulgaris L.)." Canadian Journal of Plant Science 70, no. 1 (1990): 83–89. http://dx.doi.org/10.4141/cjps90-010.

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Haploid, diploid, and tetraploid plants were regenerated from unfertilized ovule callus of the diploid (2n = 2x = 18) sugarbeet (Beta vulgaris L.) breeding line FC 607. Callus was induced by culturing the ovules on a modified Murashige and Skoog medium containing 1 mg L−1 6(γ,γ-dimethylallylamino)-purine in the dark at 27 °C. Transferring callus to the light induced shoot formation. The incidence of shoot vitrification was reduced when Gamborg’s B5 salts were substituted for Murashige and Skoog salts in the regeneration medium. Shoots rooted readily when cultured on Gamborg’s B5 salts medium c

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9

Firgiyanto, R., H. F. Rohman, M. Azizah, C. Triwidiarto, and H. Y. Riskiawan. "Effect of modified murashige and skoog medium on chrysanthemum tissue culture." IOP Conference Series: Earth and Environmental Science 1168, no. 1 (2023): 012008. http://dx.doi.org/10.1088/1755-1315/1168/1/012008.

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Abstract Propagation of chrysanthemum through tissue culture can produce superior plants in large and uniform quantities and sterile cultures (motherstock) can be obtained so that they can be used as material for further propagation. This research was carried out at Tissue Culture Laboratory, Jember State Polytechnic from April to November 2022. The research design used was a Factorial Completely Randomized Design (CRD). The first factor is the variety which consists of 3 cultivar (Maruta Agrihort, Pinka Pinki and Suciono). The second factor was in vitro culture media consisting of 5 levels (c

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Dohnal, Barbara. "Investigations on some metabolites of Tecoma stans Juss. callus tissue. Part I. Tissue culture." Acta Societatis Botanicorum Poloniae 45, no. 1–2 (2015): 93–100. http://dx.doi.org/10.5586/asbp.1976.008.

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Callus tissue satisfactorily growing was established from <i>Tecoma stans</i> Juss. seedlings in static and suspension cultures on Murashige medium modified by Mei-Lie-Lin (M-L) and on Murashige-Skoog Revised Tobacco Medium supplemented with 0.3 ppm kinetin (RT-k). Faster growth, better growth efficiency and higher anatomical organization of the cultured tissue were observed on RT-k medium.

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Uddin, M. Rafique, Martin M. Meyer Jr., and J. J. Jokela. "Plantlet production from anthers of Eastern cottonwood (Populusdeltoïdes)." Canadian Journal of Forest Research 18, no. 7 (1988): 937–41. http://dx.doi.org/10.1139/x88-142.

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Plantlets were obtained by organogenesis from cultured anthers of Populusdeltoides (Bartr.). Anthers formed callus in the dark on modified Murashige and Skoog medium supplemented with 9.0 μM 2,4-dichlorophenoxyacetic acid and 4.7 μM kinetin. Anther calli were differentiated into shoots by sequential transfer in the light onto Murashige and Skoog medium containing 4.4 μM benzylamino purine and 1.1 μM naphthaleneacetic acid for 4 weeks, followed by several transfers to woody plant medium with 2.2 μM benzylamino purine and 1.1 μM naphthaleneacetic acid. The shoots that formed were rooted by excis

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12

Qu, Yong, and Colin R. Norton. "Micropropagation of Meconopsis befonicifolia Franch. from Immature Seeds." Journal of Environmental Horticulture 4, no. 3 (1986): 74–76. http://dx.doi.org/10.24266/0738-2898-4.3.74.

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A system for micropropagation of Meconopsis betonicifolia Franch. was developed. Strength of Murashige and Skoog salts, 2,4-dichlorophenoxyacetic acid and cytokinin were varied to determine optimal treatments. Seedlings were used as explant material after root removal. One-third-strength Murashige and Skoog media produced greater survival than the same medium at full strength. Two or 5 mg 1−1 benzyladenine, kinetin or isopentenyladenine combined with 0.2 mg 1−1 2,4-dichlorophenoxyacetic accid induced the formation of multiple meristems and multiple shoots in Meconopsis. In the second subcultur

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13

Abdullah, Abdullah, Cindy Lestari, and Sudirman Numba. "PENGARUH AIR KELAPA MUDA TERHADAP PERTUMBUHAN KALUS SECARA IN VITRO DUA VARIETAS TANAMAN TEBU (Saccaharum officinarum L.)." AGROTEK: Jurnal Ilmiah Ilmu Pertanian 8, no. 1 (2024): 1–8. http://dx.doi.org/10.33096/agrotek.v8i1.470.

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This work aimed to study the in vitro effect of young coconut water in Murashige and Skoog (MS) media on callus growth of two varieties of sugarcane (Saccaharum officinarum L.). This research was conducted at the Tissue Culture Laboratory of the research and development unit of PTPN XIV Camming Sugar Factory, Bone Regency, and using a Completely Randomized Design (CRD). The treatment consisted of young coconut water concentrations of 0%, 10%, 20%, and 30%, as well as two varieties of sugarcane, namely PSBM 901 and Kidang Kencana. Data were analyzed statistically by F-test and BNJ α 0.5%. The r

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14

Adelberg, Jeffrey W., Maria P. Delgado, and Jeffery T. Tomkins. "Spent medium analysis for liquid culture micropropagation of Hemerocallis on Murashige and Skoog medium." In Vitro Cellular & Developmental Biology - Plant 46, no. 1 (2009): 95–107. http://dx.doi.org/10.1007/s11627-009-9247-1.

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15

VOICU, Diana, Simona NEAGU, Anca Ioana LUCACI, Roxana COJOC, Robert RUGINESCU та Madalin ENACHE. "IN VITRO CULTURE AND ANTIMICROBIAL ACTIVITY OF Ocimum basilicum L. var. ʻSpicy globeʼ AND Artemisia eriantha Ten." AgroLife Scientific Journal 11, № 1 (2022): 259–64. http://dx.doi.org/10.17930/agl2022130.

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In this preliminary screening, we assessed the antibacterial activity of in vitro obtained plant material, namely shoots of Artemisia eriantha (Asteraceae) and callus of Ocimum basilicum L. var. ʻSpicy globeʼ, against two bacteria strains, the Gram-positive ones including Staphylococcus aureus ATCC 25923, and Gram-negative, Escherichia coli ATCC 25922 by the plate-counting method. Artemisia sprouted on the Murashige-Skoog medium added with 1.5 mg/L benzylaminopurine and ʻSpicy globeʼ basil developed callus on Murashige - Skook basal medium supplemented with 2.5 mg/L naphtylacetic acid (NAA). T

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16

Mederos, Sebastiana, Lucía San Andrés, and Javier G. Luis. "Rosmanol controls explants browing of Hypericum canariensis L. during the in vitro estabilishment of shoots." Acta Societatis Botanicorum Poloniae 66, no. 3-4 (2014): 347–49. http://dx.doi.org/10.5586/asbp.1997.042.

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An efficient method for eradication of browning exudate was developed for <em>Hypericum canariensis</em>. For this purpose the effect of natural products on browning exudates were investigated in four types of culture media: Murashige and Skoog (MS, 1962); Gamborg's (B5, 1979); Woody Plant Medium (WPM, Lloyd and McCown 1981) and modified Quoirin and Lepoivre (QL.4) (Mederos 1991, Mederos et al. 1995) basal macroelements; these basal macroelements were supplemented with the microelement formula described by Murashige and Skoog (MS, 1962). During the establishment of shoots organogen

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17

Arif, Hussain Bhat, Alia Aparna, Mustafa Rather Ghulam, and Kumar Bharty. "Effect of NAA and BAP on In-Vitro Micropropagation of Vitex negundo L. - A Significant Medicinal Plant." RESEARCH REVIEW International Journal of Multidisciplinary 4, no. 2 (2019): 539–43. https://doi.org/10.5281/zenodo.2580250.

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Vitex negundo has been used worldwide in pharmaceutical industry. Its natural vegetative propagation rate is unable to meet growing demands of pharmaceutical industries. The present study is aimed to find a highly effective and a rapid method for the Micropropagation of this medicinal plant to meet the demand. The explants of V. negundo were cultured on Murashige and Skoogs (MS) medium supplemented with different concentrations of auxins, cytokinins and sucrose. Among different combinations used, Murashige and Skoog medium with 0.5 (mg L–1) 6-benzylaminopurine + 0.5 (mg L–1) &alpha

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18

Mello, Marcia O., Murilo Melo, and Beatriz Appezzato-da-Glória. "Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe." Brazilian Archives of Biology and Technology 44, no. 2 (2001): 197–203. http://dx.doi.org/10.1590/s1516-89132001000200014.

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Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the sam

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Górecka, K., W. Srzednicka, and L. S. Jankiewicz. "Tissue culture of horse-radish (Cochlearia armoracia L.) meristems: sterilization of buds and comparison of media." Acta Agrobotanica 31, no. 1–2 (2015): 195–204. http://dx.doi.org/10.5586/aa.1978.018.

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The attempt was made to cultivate horse-radish meristems taken from buds removed from roots. The lowest per cent of contamination was found after the buds had been soaiked in 80% ethanol for 6 minutes and then in 5%, 7.5% or 10% chloramine for 30, 30 or 15 minutes, respectively. Both agar media: Murashige-Skoog and Linsmaier-Skoog, were equally good, providing a moderate number of developing plants. The Linsmaier-Skoog medium was more satisfactory when solidified with agar; the results with liquid medium and filter-paper bridges were not as good.

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López-Puc, Guadalupe, Adriana Canto-Flick, Felipe Barredo-Pool, et al. "Direct Somatic Embryogenesis: A Highly Efficient Protocol for In Vitro Regeneration of Habanero Pepper (Capsicum chinense Jacq.)." HortScience 41, no. 7 (2006): 1645–50. http://dx.doi.org/10.21273/hortsci.41.7.1645.

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To induce somatic embryogenesis in habanero pepper (Capsicum chinense Jacq.), the cultivar BVll-03, belonging to the red type, was used. Different explants were evaluated, as were different culture media, the composition of which varied in the content of plant growth regulators. Results showed the formation of somatic embryos from cotyledons, zygotic embryos, germinated zygotic embryos, hypocotyls, and cotyledonary leaves. Explants were cultured on Murashige and Skoog medium supplemented with 2,4-D (9.05 μm). The somatic embryos always formed directly from the explant, without callus formation

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ALARCÓN P., Juan C., Diego M. MARTINEZ M., and Andra SALAZAR-OSPINA. "<I>In vitro</I> PROPAGATION OF <I>Heliconia curtispatha</I> P, PLANT USED AGAINST SNAKEBITE BY SOME RURAL COMMUNITIES OF THE COLOMBIAN REGION OF URABÁ." Vitae 18, no. 3 (2011): 271–78. http://dx.doi.org/10.17533/udea.vitae.10650.

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Heliconia curtispatha Petersen (Zingiberales) commonly known as platanillo, is used in colombian traditional medicine by its anti-edema, antihaemorrhagic and neutralising action of Bothrops asper (mapaná, X) venom, responsible for 95% of snakebites in the country. The previous, emphasizes its utility and potential function as helper in the treatment of the ophidian accident. The in vitro propagation techniques becomes an interesting tool for plant and metabolites production, because of the hazardous accessibility to plants in their natural environment and the deficiency of previous studies on

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Kathiravan, K., A. Shajahan, and A. Ganapathi. "REGENERATION OF PLANTLETS FROM HYPOCOTYL DERIVED CALLUS OF MORUS ALBA." Israel Journal of Plant Sciences 43, no. 3 (1995): 259–62. http://dx.doi.org/10.1080/07929978.1995.10676610.

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Plantlets were regenerated from hypocotyl callus of Morus alba cv. MR2. Calli were established from hypocotyl segments on Murashige and Skoog (MS) medium supplemented with indoleacetic acid (0.5 mg/1) and benzyladenine (BA) (0.5 mg/1). They were transferred to MS medium with different concentrations of naphthaleneacetic acid NAA and BA for four weeks. Adventitious shoot buds were observed by transferring callus onto fresh Linsmaier and Skoog (LS) medium containing NAA (0.5 mg/1) and BA (0.75 mg/1). Shoots produced in vitro were rooted on MS medium with indolebutyric acid (0.75 mg/1).

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Aryal, Saraswoti, and Sanu Devi Joshi. "Callus Induction and Plant Regeneration in Rauvolfia Serpentina (L.) Benth Ex. Kurz." Journal of Natural History Museum 24 (October 9, 2009): 82–88. http://dx.doi.org/10.3126/jnhm.v24i1.2245.

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Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing

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Malahayati, Siti, Noval Noval, and Setia Budi. "Inisiasi Protocorm Like Bodies (PLB) Dendrobium sylvanum." Journal Pharmaceutical Care and Sciences 2, no. 2 (2022): 39–50. http://dx.doi.org/10.33859/jpcs.v2i2.184.

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Anggrek genus Dendrobium menghasilkan metabolit sekunder yang berkhasiat obat yaitu alkaloid, alkaloid yang utama pada Dendrobium yaitu dendrobine. Bagian tanaman anggrek yang mengandung metabolit sekunder dalam jumlah besar yaitu dalam bentuk protocorm like bodies (PLB). Pada penelitian ini dilakukan inisiasi PLB dari eksplan biji anggrek Dendrobium sylvanum. Penelitian ini bertujuan untuk mengetahui konsentrasi giberelin dalam medium Murashige and Skoog untuk inisiasi PLB yang optimal. Kultur biji dilakukan pada medium Murashige and Skoog dengan penambahan zat pengatur tumbuh giberelin (GA3)

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Yu, Chang-Yeon, and John Masiunas. "IMPROVED PLANT REGENERATION OF SOLANUM AND LYCOPERSICON GENOTYPES FROM LONG-TERM CALLUS CULTURE." HortScience 25, no. 9 (1990): 1121b—1121. http://dx.doi.org/10.21273/hortsci.25.9.1121b.

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Repeated callus sub-culture reduce the regeneration capacity in many species. Our studies determined the effect of genotype and medium on regeneration of several Solanum and Lycopersicon genotypes from long-term callus cultures. In the first study, 13 genotypes were transferred to regeneration medium, including: Murashige and Skoog plus Gamborg Vitamins (MG); Murashige and Skoog (MS); Gamborg (GM); and white (WM). The greatest shoot regeneration was on the MG medium, containing the highest levels of thiamine. Shoot differentiation was greatest with 0.2 mg/l IAA and 2 mg/l BA. No plants were re

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Mehta, Nikhil, Priyanka Rao, and Raman Saini. "Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata." Agriculture (Pol'nohospodárstvo) 67, no. 3 (2021): 95–102. http://dx.doi.org/10.2478/agri-2021-0009.

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Abstract Sesbania aculeata is a multipurpose legume crop grown primarily for green manuring in the rice-based cropping system. Besides this, it is an industrial crop and is also used as food in many parts of the world. The present work reports for optimization of various parameters (growth medium, plant growth regulators, pre-conditioning, orientation of explant, and presence of thiol compounds) affecting in vitro regeneration using mature cotyledon explants. The 5-day-old mature cotyledon explants excised from seedlings grown on Murashige and Skoog (MS) salts and Gamborg (B5) vitamins medium

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Hunková, Júlia, Gabriela Libiaková, Jozef Fejér, Tatjana Vujovic, and Alena Gajdosová. "Testing of different iron sources and concentrations on shoot multiplication of blackberry (Rubus fruticosus L.)." Genetika 50, no. 1 (2018): 351–56. http://dx.doi.org/10.2298/gensr1801351h.

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The aim of this work was to evaluate shoot multiplication of two blackberry (Rubus fruticosus L.) cultivars ?Black Satin? and ?Loch Ness? on two culture media: Murashige & Skoog (MS) and its modification Murashige & Skoog Van der Salm medium (MS VDS) which differ only in iron source (FeNaEDTA, FeEDDHA, respectively). Statistical analyses showed no significant difference in shoot multiplication between different iron sources for both tested cultivars. For ?Black Satin? it was shown that double concentration of chelates FeNaEDTA and FeEDDHA in culture media negatively affected on shoot g

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21., Prashantha K. G. Sathyanarayana B. N. and Deepu Mathew. "In vitro shoot proliferation and plant regeneration in sweet carambola (Averrhoa carambola)." Tropical Science 44 (September 10, 2004): 108–10. https://doi.org/10.1002/ts.147.

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Sweet carambola can be propagated by nodal explants from seedlings cultured on Murashige and Skoog medium containing growth-promoting agents. The best combination was 2.0 mg/l benzylaminopurine and 0.2 mg/l naphthylacetic acid.

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Siatka, Tomáš. "Production of Anthocyanins in Callus Cultures of Angelica archangelica." Natural Product Communications 13, no. 12 (2018): 1934578X1801301. http://dx.doi.org/10.1177/1934578x1801301219.

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Anthocyanins have been used as food color additives, but they also possess many properties beneficial to health. Plant tissue culture technology is an attractive alternative for obtaining these valuable natural pigments. In this work, dark-grown anthocyanin producing callus cultures of Angelica archangelica were established. They were cultured on a Murashige and Skoog medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid and 0.4 mg/L benzylaminopurine. Anthocyanin contents in cultures were around 2%, i.e. one order of magnitude higher than in the intact plant that contains up to 0.17%

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Jan, Mahroofa, Seema Singh, Zahoor A. Kaloo, and Farhana Maqbool. "Callus induction and multiple shoot regeneration in Ajuga bracteosa Wall ex. Benth.-An important medicinal plant growing in Kashmir Himalaya." Journal of Scientific and Innovative Research 3, no. 3 (2014): 319–24. http://dx.doi.org/10.31254/jsir.2014.3308.

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Ajuga bracteosa Wall ex. Benth is an important medicinal plant growing in Kashmir Himalaya. During the present study an efficient and rapid in vitro protocol has been established viz; Callus induction and multiple shoot regeneration. Callus induction has been achieved from different explants viz., leaf, petiole and internodal cuttings. Maximum callus production was obtained when leaf explants were inoculated on Murashige and Skoog (1962) medium supplemented with BAP (benzylaminopurine 5.0 mg/l) after 19 days of inoculation. In the petiole explant callus was induced on MS (Murashige and Skoog )

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MARKOVIĆ, Marija, Mihailo GRBIĆ, and Matilda DJUKIĆ. "Micropropagation of the Endangered and Decorative Specie Dianthus serotinus Waldst. et Kit." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41, no. 2 (2013): 370. http://dx.doi.org/10.15835/nbha4129265.

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During past decades, great attention has been paid to propagation of endangered plant taxa in order to preserve biodiversity. The aim of this study was to optimize a protocol for in vitro propagation of the critically endangered and decorative species Dianthus serotinus Waldst. et Kit. The effects of different concentration of MS salt (Murashige and Skoog) of the culture, medium pH and different carbohydrates (sucrose, glucose, and fructose) on shoot multiplication were examined. The best results were obtained on half-strength MS (Murashige and Skoog) medium, whose pH was 5.8, with sucrose sup

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Ștefan, Maria, Andreea Tican, Carmen Bădărău, and Delia Paraschiv. "Identification of Potato Breeding Lines with Tolerance to Hydric Stress under in vitro Conditions." Romanian Agricultural Research 37 (2020): 99–106. http://dx.doi.org/10.59665/rar3713.

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In this research our aim was to identify genotypes with hydric stress tolerance in vitro among 44 potato breeding lines. The study involved the number of leaves / plantlet, plantlet length and root growth of plantlets. Polyethylene glycol (6000) was used for exerting the hydric deficiency stress on the in vitro plantlets. Different levels of hydric stress were induced using two concentrations (1% and 2%) of polyethylene glycol (PEG) added in Murashige-Skoog medium. The values obtained for the mentioned traits analyzed on medium with PEG were compared with Murashige-Skoog medium (control). Unde

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Dang, Giang Thi Quynh, Bac Thien Van, and Hoang Ngo Phan. "CULTURING THE SHOOT TIP FROM TUBER SHOOTS OF CALLA LILY (Zantedeschia eliottiana Engl.)." Science and Technology Development Journal 12, no. 17 (2009): 64–70. http://dx.doi.org/10.32508/stdj.v12i17.2368.

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Tuber shoots are excised and cultured onto MS medium (Murashige & Skoog, 1962). Shoot tips are isolated from in vitro plantlets and grow in a stable way on medium supplemented with different plant growth regulations, among the medium, shoot tips arise most on MS medium supplemented with 2mg/l BA and 0.5mg/l IBA. Role of endogenous hormones, respiration rate and origin of shoot formation were analysis.

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Shevaha, H. M., M. M. Kyryk, V. M. Hunchak, and T. M. Oliinyk. "THE OPTIMIZATION OF JELLIFYING AGENT COMPOSITION ON REGENERATION OF IN VITRO OBTAINED POTATO MICROPLANTS." Agriciltural microbiology 24 (October 9, 2016): 79–82. http://dx.doi.org/10.35868/1997-3004.24.79-82.

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Results of studies on the optimization of Murashige and Skoog culture medium withmodified maize starch for the acceleration of potato plants micropropogation are provided. Ithas been established that the method modification increases surveillance parameters, promotesactive in vitro growth and development of potato springs.

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Tóth, Szilárd, and Pál Pepó. "Nutrient Uptake of Miscanthus in vitro Cultures." Acta Agraria Debreceniensis, no. 1 (May 12, 2002): 23–24. http://dx.doi.org/10.34101/actaagrar/1/3531.

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The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content

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Miachir, Jeanette Inamine, Vera Lúcia Moretti Romani, Antônio Francisco de Campos Amaral, Marcia Ometto Mello, Otto Jesu Crocomo, and Murilo Melo. "Micropropagation and callogenesis of Curcuma zedoaria Roscoe." Scientia Agricola 61, no. 4 (2004): 427–32. http://dx.doi.org/10.1590/s0103-90162004000400012.

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Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis of Curcuma zedoaria Roscoe as alternative to improve plant production, turning economically feasible the exploitation of its secondary metabolites which present medicinal properties. Micropropagation by using shoot apexes produced by rhizome and from in vitro plants were carried out on Murashige & Skoog medium supplemented with 2.0 mg L-1 benzyl amino purine an

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Romeida, A., D. W. Ganefianti, M. Marlin, et al. "Protocorm Like Bodies (PLBs) dendrobium orchid “Gatton Sunray”." E3S Web of Conferences 373 (2023): 03002. http://dx.doi.org/10.1051/e3sconf/202337303002.

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The Dendrobium Orchid Gatton Sunray is a hybrid orchid of Dendrobium pulchellum × Dendrobium illustre. A study was aimed to find the best type of medium and type of banana for the multiplication of Protocorm-Like Bodies (PLBs) of this orchid. This research was conducted from February to September 2020 at the Plant Tissue Culture Laboratory, Faculty of Agriculture, The University of Bengkulu. Treatments included type of media and cultivar of ripe banana fruit, added as source of organic complex, consisted of 4 cultivars, namely Ambon Curup, Kepok, Tanduk and Mas. Ripe bananas were mashed and ad

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Saminathan, M., A. Muruganandam, M. Arumugam, and Amzad Basha Kolar. "Micropropagation and assessment of genetic fidelity of Decalepis hamiltonii Wight & Arn using RAPD markers." Biochemical and Cellular Archives 23, no. 2 (2023): 721–26. http://dx.doi.org/10.51470/bca.2023.23.2.721.

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Somaclonal variation in micropropagated plants improves crops. Genetic transformation research relies on micropropagated plant genetic integrity. Somaclonal variation frequently occurs in micropropagation, prompting an investigation of plant genetic stability. The high number of shoot buds was seen when Decalepis hamiltonii shooting tip explants were inoculated with reversed polarity in Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 benzylaminopurine (BAP). The shoot buds increased in length on an MS medium supplemented with 0.5 mg L-1 Benzylaminopurine (BAP) and 0.1 mg L-1 indol

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Hutsko, K. I. "OBTAINING in vitro CALLUS BIOMASS OF AMARANTH VARIETY “LERA”." Biotechnologia Acta 18, no. 2 (2025): 41–44. https://doi.org/10.15407/biotech18.02.041.

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Aim. To obtain callus biomass of the amaranth variety “Lera” in vitro and determine the optimal concentration of growth regulators in the nutrient medium for maximum callus formation. Methods. The study was conducted using in vitro cell culture. Cultivation was performed on a modified Murashige-Skoog medium supplemented with benzylaminopurine and naphthylacetic acid at various concentrations. Experimental conditions: photoperiod 16/8 h (light/darkness), temperature 20±1°C, illumination 2000 lux, relative humidity 70%. The total cultivation duration was 56 days. Results. The seed sterilization

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Al-Juboory, Karim H. "REGENERATION IN VITRO OF CUCUMBER (CUCUMIS SATIVUS L.) CULTURED FROM EXCISED SEEDS AND COTYLEDONS." HortScience 28, no. 5 (1993): 499c—499. http://dx.doi.org/10.21273/hortsci.28.5.499c.

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Seed and cotyledon explants of `Al-Mokhtar' hybrid (a new cultivar of cucumber) were prepared and disinfected. Explants were transferred to modified Murashige and Skoog (MS) medium supplemented with a combination of 6-benzylaminopurine (BA) and napthaleneacetic acid (NAA). Shoots regenerated best on medium supplemented with ≥ 2.0 mg/l BA. The BA concentration significantly interacted with NA with respect to shoot regeneration.

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Parris, J. Kevin, Darren H. Touchell, Thomas G. Ranney, and Jeffrey Adelberg. "Basal Salt Composition, Cytokinins, and Phenolic Binding Agents Influence In Vitro Growth and Ex Vitro Establishment of Magnolia ‘Ann’." HortScience 47, no. 11 (2012): 1625–29. http://dx.doi.org/10.21273/hortsci.47.11.1625.

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In vitro growth responses of Magnolia ‘Ann’ to basal salt composition, cytokinins, and phenolic binding agents were investigated in a series of experiments to refine micropropagation protocols. Murashige and Skoog (MS), half-strength MS, Woody Plant Medium (WPM), Driver and Kuniyuki (DKW), and Blaydes basal salts in conjunction with 1 g·L−1 activated charcoal (AC) or 1 g·L−1 polyvinylpyrrolidone (PVP) were evaluated as multiplication media. Benzylaminopurine (BAP), meta-topolin (mT), or 6-(γ,γ-dimethylallylamino) purine (2iP) at 2, 4, or 8 μM was investigated to optimize the cytokinin concentr

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Wickremesinhe, E. R. M., W. J. Blackmon, and B. D. Reynolds. "In Vitro Propagation of Apios americana." HortScience 25, no. 11 (1990): 1439–40. http://dx.doi.org/10.21273/hortsci.25.11.1439.

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Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

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Mukherjee, Pranit Kumar, Raju Mondal, Sourav Dutta, Kanti Meena, Madhumita Roy, and Asit Baran Mandal. "In vitro micropropagation in Boehmeria nivea to generate safe planting materials for large-scale cultivation." Czech Journal of Genetics and Plant Breeding 54, No. 4 (2018): 183–89. http://dx.doi.org/10.17221/79/2017-cjgpb.

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An efficient in vitro micropropagation protocol has been developed using nodal explants of ramie (Boehmeria nivea), with maximum shoots (42) per explant in 5 passages (passage duration: 21 days) on Murashige and Skoog medium supplemented with 2.0 mg/l 6-benzyladenine and 2.0 mg/l AgNO<sub>3</sub>. ½ Murashige and Skoog medium containing 40% sucrose was found to be most effective for the rooting of in vitro developed shoots. Those plantlets were acclimatized and transferred to pots for hardening under glasshouse conditions. About 91% of mericlones survived and showed no ectopic expr

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Dubceac, Marcela, and Victor Bondarciuc. "Evaluarea influenței fitohormonilor IAA și 2IP asupra dezvoltării viței-de-vie (Vitis vinifera) în cultura in vitro." Pomicultura, Viticultura şi Vinificaţia, no. 2(92) (December 2024): 14–19. https://doi.org/10.53082/1857-3142.24.92.03.

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In this study, the effects of supplementing nutrient media with indole-3-acetic acid (IAA) and 6-(γ,γ-Dimethylallylamino) purine (2iP) on the development of grapevine plantlets in vitro were investigated. Four variants of the Murashige–Skoog medium with various concentrations of phytohormones were analyzed to evaluate their impact on growth parameters such as root biomass, leaf biomass, the number of lateral shoots, plant height, and internode formation. The results of the research showed the best development of grapevine plants in in vitro culture. The most favorable development of grapevine

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Kato, Michiyo. "Polyploids of Camellia through Culture of Somatic Embryos." HortScience 24, no. 6 (1989): 1023–25. http://dx.doi.org/10.21273/hortsci.24.6.1023.

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Abstract Somatic embryos of camellia (Camellia japonica L.) were differentiated from single cells of hypocotyl explants placed on Murashige and Skoog medium containing GA3. When these somatic embryos were treated with 0.1% colchicine for 1 week, secondary polyploid embryos formed on the hypocotyl of primary embryos. On MS + GA3 medium, polyploid embryos proliferated in a similar manner during subsequent subcultures. These polyploid embryos developed into plantlets that exhibited autopolyploid morphological characteristics.

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Hidayat, Rahmad, Abdullah Abdullah, and Aminah Aminah. "PEMANTAPAN MASA INKUBASI KALUS KEDELAI SECARA IN VITRO DALAM MEDIA MS (Murashige dan Skoog) B5 (Gamborg)." AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian 2, no. 2 (2022): 60–70. http://dx.doi.org/10.33096/agrotekmas.v2i2.192.

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Combination of Soybean Callus Incubation Period in vitro in MS (Murashige and Skoog) and B5 (Gamborg) Media (Supervised by Abdullah and Aminah). This study aimed to find a suitable medium for stabilizing the incubation period of soybean callus in vitro for the purposes of further selection. This research was conducted at the Laboratory of Bio-Science and Plant Reproduction Biotechnology, Agronomy Department, Agriculture Faculty, Hasanuddin University, Makassar, South Sulawesi. This research was done from June 2020 to October 2020. This research was conducted using a Completely Randomized Desig

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Kubicki, B., J. Telżyńska, and E. Milewska-Pawliczuk. "Induction of embryoid development from apple pollen grains." Acta Societatis Botanicorum Poloniae 44, no. 4 (2015): 631–35. http://dx.doi.org/10.5586/asbp.1975.057.

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Globular 32 and 64 celled embryoids were obtained from uninucleate apple microspores (cultivar Jonathan) after 5 weeks of culture on a modified Murashige and Skoog (1962) medium. A similar induction of microspore development was not observed in younger or older stages of anther development. In such anthers only callus was formed from diploid tissues.

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Cockrel, Angela D., G. L. McDaniel, and E. T. Graham. "In Vitro Propagation of Florists’ Cineraria." HortScience 21, no. 1 (1986): 139–40. http://dx.doi.org/10.21273/hortsci.21.1.139.

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Abstract Tissue culture propagation of Senecio cruentus DC. ‘Hansa’ was achieved from shoot tips derived from selected seedlings germinated in vitro. Shoot multiplication was best accomplished on Murashige and Skoog (MS) gerbera medium supplemented with 2.8 μM IAA. Maximum root formation occurred on MS modified medium with reduced salt levels and 1.3 μM NAA. Rooted shoots were established successfully in a soilless medium under high humidity. Chemical names used: 1H-indole-3-acetic aid (IAA); 1-naphthaleneacetic acid (NAA).

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Cvetic, Tijana, Aneta Sabovljevic, M. Sabovljevic, and D. Grubisic. "Development of the moss Pogonatum urnigerum (Hedw.) P. Beauv. under in vitro culture conditions." Archives of Biological Sciences 59, no. 1 (2007): 57–61. http://dx.doi.org/10.2298/abs0701057c.

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Pogonatum urnigerum (Polytrichaceae) in vitro culture was established from spores collected in nature. Both protonema and gametophore stages of gametophyte development were obtained. Also, a stable callus culture was established using hormone-free nutrient medium. The best nutrient medium for development was half-strength Murashige- Skoog medium supplemented with 1.5% sucrose. Auxin treatment enabled some gametophores to develop, but prolonged treatment induced early senescence. Tissues grown on cytokinin did not produce any gametophytes and did not survive prolonged treatment.

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MAZRI, Mouaad Amine. "Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’." Notulae Scientia Biologicae 5, no. 3 (2013): 332–37. http://dx.doi.org/10.15835/nsb539089.

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The effect of basal medium, explant size and density on shoot multiplication, growth, rooting and acclimatization of date palm cv. ‘16-bis’ was evaluated. Bud clusters of different sizes (2, 3, 4 and 5 buds per cluster) were cultured at density of 1, 2, 3 and 4 clusters on Murashige and Skoog medium (MS), woody plant medium (WPM) and Nitsch medium (NM) supplemented with 0.5 mg/L 2-naphthoxyacetic acid and 0.5 mg/L kinetin for three months (multiplication phase). Separated shoots of different sizes (

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