Academic literature on the topic 'Mushroom extract'
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Journal articles on the topic "Mushroom extract"
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Iermolenko, T. I., N. G. Ruda, and O. I. Pautina. "COMPARATIVE STUDY OF ANTI-INFLAMMATORY, ANALGESIC AND ANTI-ULCER EFFECT OF CANCARIUS MUSHROOM EXTRACT (CANTHARELLUS CIBARIUS) AND SHIITAKE MUSHROOM EXTRACT (LENTINUS EDODES) IN THE EXPERIMENT." Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії 21, no. 2 (2021): 135–41. http://dx.doi.org/10.31718/2077-1096.21.2.135.
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Mushrooms are appreciated not only for their nutritional value and bioavailability, but also for their medicinal properties. There are a growing number of the studies focused on investigating medical preparation base on natural compounds, including those isolated from a number of fungi species of fungi species. The aim of this study is to compare anti-inflammatory, analgesic and antiulcer effects of extracts of shiitake mushrooms and chanterelle mushrooms in an experiment on rats. Materials and methods. The experiment involved 30 white female rats weighing 170-210 g., divided into 5 groups: group 1 included intact animals; group 2 included animals with modelled pathology; group 3 included animals, which received chanterelle extract; group 4 involved animals, which received shiitake mushroom extract, and group-5 rats received the reference drug. Observation of changes over the inflammatory process in the model of carrageenan-induced inflammation in the paw of the rats revealed an anti-exudative effect of varying intensity that made up 36.84% for the of chanterelle mushroom extract and 15.79% for shiitake mushroom extract. The dynamics of trypsin-induced inflammatory process showed moderate anti-inflammatory activity, 32.02%, during the courses of the therapy with chanterelle mushroom extract. According to the indicator of analgesic effects produced by the studied extracts, the highest indicator was reached by the chanterelle mushroom extract (33.53%) that exceeded the analgesic effect of the shiitake mushroom extract (17.65%). Therapeutic and prophylactic administration of the chanterelle mushroom extract in a dose of 10 mg / kg in the model of aspirin-induced gastric ulcer in rats showed a pronounced anti-ulcer effect which made up 66.7% compared with that by shiitake mushroom extract (33.4%) and the reference drug “Altan” (100%). Conclusion. The obtained data have demonstrated the promise of using the extracts of chanterelle and shiitake mushrooms for the therapeutic and prophylactic purposes.
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Chanate, Wanna, Boonman Narumon, Phakpaknam Sirirat, Chutrtong Jaruwan, and Wongwiwat Piyada. "Comparative Evaluation of Chemical Compositions and Antioxidant Activity in Fruiting Bodies and Mycelia of Volvariella volvacea and Pleurotus sajor-caju Extracts." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 15, no. 03 (2024): 1499–509. http://dx.doi.org/10.25258/ijpqa.15.3.63.
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Mushrooms, recognized for their rich medicinal and nutritional content, have garnered significant interest due to their potent antioxidant properties. This study investigates the fruiting bodies and mycelia of straw mushrooms (Volvariella volvacea) and grey oyster mushrooms (Pleurotus sajor-caju). The mushrooms were extracted using distilled water at ratios of 1:25, 1:30, and 1:35 g/mL by soaking in a temperature-regulated shaking bath set at 50°C. The aqueous extracts were analyzed for their bioactive components, including polysaccharides, proteins, GABA, total phenolic content (TPC), and total flavonoid content (TFC), along with their antioxidant capacities using DPPH and ABTS assays. The results indicated that the straw mushroom exhibited higher levels of polysaccharides, GABA, TPC, and TFC compared to the grey oyster mushroom, which contained higher protein levels. Specifically, the fruiting bodies extract of the straw mushroom demonstrated the highest levels of polysaccharides (416.94 mg/g extract), GABA (12.86 mg/g extract), TPC (33.27 mg GAE/g extract), and TFC (9.39 mg CE/g extract). Furthermore, the fruiting bodies extract showed a greater capacity to scavenge free radicals than the mycelia extract of both straw mushroom and grey oyster mushroom, with DPPH assay values of 0.65 and 0.47 mg TE/g extract and ABTS assay values of 2.26 and 1.74 mg TE/g extract, respectively. These results suggest that the fruiting bodies possess higher free radical scavenging potential as an outcome of their high levels of bioactive compounds. Consequently, these extracts indicate promise as natural antioxidative agents and could be effectively utilized as ingredients in the culinary industry.
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Ng, S. P., R. Ramanathan, and X. J. Chong. "The effect of cooking duration on radical scavenging properties of Hypsizygus tessellatus and Pleurotus ostreatus." Supplementary 1 6, S1 (2022): 75–79. http://dx.doi.org/10.26656/fr.2017.6(s1).013.
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Mushrooms are a popular ingredient in the human diet due to their flavour, nutritional values and functional properties, in particular, their antioxidant potential. Most mushrooms are consumed after cooking and the impact of cooking methods and durations on the antioxidant properties of various edible mushrooms have been reported. However, the reports on the effect of cooking on the antioxidant properties of Hypsizygus tessellatus (shimeji), a widely consumed mushroom in East Asia, are limited. Therefore, this study aimed to investigate the effect of cooking duration on the radical scavenging properties of H. tessellatus. Pleurotus ostreatus was included for comparison purposes. Mushroom samples were prepared raw, boiled in distilled water for 1 min, 3 mins and 5 mins, then blended and centrifuged to obtain mushroom extracts. The mushroom extracts were evaluated for their radical scavenging properties using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Results showed that 1-min cooked H. tessellatus extract exhibited the highest radical scavenging activity (76.6±2.14% DPPH scavenging activity) while extract boiled for 5 mins had the lowest radical scavenging activity (62.9±6.08% DPPH scavenging activity). Thus, cooking time has a significant effect on the radical scavenging activity of H. tessellatus extract. In conclusion, the effect of cooking on H. tessellatus and P. ostreatus can be both beneficial and disadvantageous depending on the duration.
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Sunthudlakhar, Punjaporn, Pongtip Sithisarn, Bhusita Wannissorn, Siripen Jarikasem, and Piyanuch Rojsanga. "Phytochemical Profiles, Antioxidant and Antibacterial Activities of 11 Phellinus Mushrooms Collected in Thailand." Natural Products Journal 9, no. 2 (2019): 144–56. http://dx.doi.org/10.2174/2210315508666180621160917.
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Background:Phellinus mushrooms are locally available in Thailand. They have been traditionally used for medicinal purposes including tonics, for cancer treatment and for immune system stimulation. </P><P> Objective: To evaluate the in vitro antioxidant, antibacterial activities and the phytochemical information of the extracts from 11 selected Phellinus mushrooms collected in Thailand.Methods:Eleven Phellinus mushrooms were collected from the Northeastern part of Thailand. Their ethanol and water extracts were tested for in vitro antioxidant activities using a DPPH scavenging assay, FRAP assay and TBARS method while the antibacterial effects were determined by agar disk diffusion and broth microdilution methods. The phytochemical profiles of the Phellinus mushroom extracts were evaluated using TLC and HPLC techniques. The total phenolic and total flavonoid contents of the extracts were evaluated by Folin-Ciocalteu and aluminium chloride methods, respectively. While total carbohydrate and total triterpene contents were evaluated by phenolsulfuric acid and vanillin perchloric acid methods, respectively.Results:Most ethanol extracts from Phellinus mushrooms exhibited moderate antioxidant effects especially extracts from P. everhartii, P. hippophaëicola and P. pini var. microporus while P. igniarius var. cinereus ethanol extract exhibited inhibitory effects to Helicobacter pylori both normal and resistant strains, this extract also showed the strongest inhibitory effect against Staphylococcus aureus. Most Phellinus mushroom extracts exhibited similar TLC and HPLC fingerprints with the chromatographic bands corresponding to phenolics, flavonoids and terpenoids. Protocatechuic acid was identified in most Phellinus mushroom extracts.Conclusion:Phellinus mushrooms especially, P. everhartii could be developed as sources of antioxidants while P. igniarius var. cinereus indicated the potential to inhibit H. pylori. Protocatechuic acid could be used as a marker for quality control of Phellinus mushroom raw materials and extracts.
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HAMDAN, Noor T. "FIRST REPORT OF THE ANTICANCER EFFICIENCY OF AGARICUS BRASILIENSIS MUSHROOM ON HUMAN EMBRYONIC LIVER WRL68 AND HUMAN PANCREATIC ASPC-1 CANCER CELLS THERAPY." MINAR International Journal of Applied Sciences and Technology 03, no. 02 (2021): 28–35. http://dx.doi.org/10.47832/2717-8234.2-3.4.
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Mushrooms are food traditionally consumed in Asia, Europe and America. They are being studied for medicinal benefits. Extensive studies have shown that Agaricus brasiliensis mushroom used as a medical productto combat cancers. Our data reveal that the determined inhibitory concentration fifty(IC50) values were observed maximum dose responses (IC50) of WRL68 andAsPC-1 cancer cells reported of 172.6 µg/ml and 158.2 µg/ml respectively at 2.23 and 2.1 µg/ml ethanolic mushroom concentrations. The highly cytotoxic activity of the extract on growth inhibition AsPC-1 and WRL68were generally observed 97.9% and 95% at extract concentrations of 25 µg/ml and 50µg/ml respectively. Finally, Phytochemical profile of Agaricus brasiliensis mushroom extract found to beflavonoids, glycosides, saponins, phenols,alkaloids, tannins.The extracts of Agaricus brasiliensiswas tested throughGas Chromatography-Mass(GC-MS). There were five different compounds analyzed from the extracts of Agaricus brasiliensis. The compounds in the ethanolic extract of A. brasiliensis mushroom were comprised mostly of Acetic acid ethyl ester(38.39%),followed by(3-Methyl-oxiran-2-yl)-methanol (34.71%), Chlorbromuron (22.86%), n-Hexadecanoic acid (3.85%) and Heptane, 1-(1-butenyloxy) (0.19%). However, no studies were done using Agaricus brasiliensismushroom extract against WRL68 and AsPC-1 human cell lines. Therefore, the anticancer efficiency of Agaricus brasiliensis mushroom on human embryonic liver WRL68 andhuman pancreatic AsPC-1 cancer cellstherapy is being reported for the first time in the current study.
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R, Madhanraj, Ravikumar K, Maya MR, et al. "Evaluation of anti-microbial and anti-haemolytic activity of edible basidiomycetes mushroom fungi." Journal of Drug Delivery and Therapeutics 9, no. 1 (2019): 132–35. http://dx.doi.org/10.22270/jddt.v9i1.2277.
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Mushrooms are used for a variety of biotechnological applications, particularly for the production of food, enzymes, dietary supplements, pharmaceutical compounds, feed supplements. Basidiomycetes mushroom fungi have been known for their nutritional values they can be considered as functional foods which can provide health benefits beyond the traditional nutrients. Mushroom represents a major and untapped source of potent new pharmaceutical products. A wide range of activities including antitumour, cardiovascular and antimicrobial are reported in mushrooms. In developing countries like India mushroom progress is a boon in the field of food, medicine, and in generating employment. The alternative systems of medicine utilize the curative properties of mushrooms. They are also known to possess promising antioxidative, cardiovascular, hypercholesterolemia, antimicrobial, hepato-protective and anticancer effects. The present study aimed to evaluate antimicrobial and antihaemolytic activities of various extracts of Basidiomycetes mushroom fungi. Different Basidiomycetes mushroom fungi extracts (Hot water, Acetone and Hexane) were tested for antimicrobial and antihaemolytic activities. Among these different extracts of Basidiomycetes mushroom fungi the hexane extract of Pleurotus pulmonarius showed potential antimicrobial and antihaemolytic activity. The results of this study suggest the possibility of using Basidiomycetes mushroom fungi as natural sources for the pharmaceutical industry and could act as safe and cost-effective with potential biological activities. These findings encourage studying these fungal strains further for their potential biological applications.
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Sitati, Concepta N. W., Kenneth O. Ogila, Rebecca W. Waihenya, and Lucy A. Ochola. "Phytochemical Profile and Antimicrobial Activities of Edible Mushroom Termitomyces striatus." Evidence-Based Complementary and Alternative Medicine 2021 (October 19, 2021): 1–10. http://dx.doi.org/10.1155/2021/3025848.
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The mushroom Termitomyces striatus is an edible mushroom that grows wildly and belongs to the family Lyophyllaceae. Studies in the last few decades have demonstrated that mushrooms and their active components have beneficial effects on a variety of biological systems. Some mushrooms do exhibit antibacterial properties. Qualitative phytochemical profile was done on the mushroom Termitomyces striatus to establish the presence of compounds responsible for important biological activities. This study also investigated the effect of Termitomyces striatus extracts on certain bacterial strains that included Escherichia coli and Pseudomonas aeruginosa representing the Gram-negative bacteria and Bacillus subtilis and Staphylococcus aureus representing Gram-positive bacteria. The fungi were represented by Candida albicans and Saccharomyces cerevisiae. The mushroom was collected in western Kenya, air-dried, and crushed into powder, followed by extraction using water, methanol, and dichloromethane (DCM) solvents. Antibacterial and antifungal activities were evaluated using the disc-diffusion method. Qualitative phytochemical screening of the aqueous extract revealed the presence of alkaloids, flavonoids, steroids, sterols, saponins, phenols, carbohydrates, and proteins. The three extracts exhibited antibacterial against tested bacterial strains. The DCM extract revealed higher effects among the bacterial strains tested. The three extracts showed antifungal effects against C. albicans. However, both methanol and aqueous extracts did not inhibit growth of S. cerevisiae. In conclusion, T. striatus extracts are a promising source of novel antimicrobial and antifungal agents.
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Nurkanti, Mia, Mimi Halimah, Yosi Silfister, and Handi Suganda. "Potensi Ekstrak Lengkuas (Alpinia Purpurata K. Schum) Sebagai Fungisida Terhadap Jamur Alternaria Sp. Pada Tanaman Jeruk." Biotropic : The Journal of Tropical Biology 4, no. 2 (2020): 111–18. http://dx.doi.org/10.29080/biotropic.2020.4.2.111-118.
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Alternaria sp. is one the causess of diseases that attack citrus crops. Pest and diseases control efforts by farmers more often use pesticide synthesis. This study aims to determine the inhibition of mushroom growth and other benefits of galangal extract as a natural fungicide and determine the optimum concentration of galangan extract inhibiting the growth mushroom of Alternaria sp.. The method used was experimental, testing conducted by growing mycelium mushroom Alternaria sp. on a PDA that has been mixed with extract of galangal in according to the respective concentrations and analyze growth response by measuring the diameter. The best results indicated by the treatments 60% that control the growth of mushrooms with deaths amounted to 79%. This shown that galangal extract is able to control the growth of Alternaria sp. at concentrations 60% compared to a concentration of 20%, 30%, 40% and 50% because the active compounds at concentrations that are 60% higher, so galangal extract diffuse into the cell the growing of mushrooms and may cause disruption the growth mushrooms Alternaria sp.. The higher concentration of extract given, the ability to controlling the growth mushroom of Alternaria sp. the better. The utilization of galangal material is done as an alternative fungicide in the control of plant diseases that are eco friendly.
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Bakır, Temel kan, Mansor Boufars, Mertcan Karadeniz, and Sabri Ünal. "AMINO ACID COMPOSITION AND ANTIOXIDANT PROPERTIES OF FIVE EDIBLE MUSHROOM SPECIES FROM KASTAMONU, TURKEY." African Journal of Traditional, Complementary and Alternative medicines 15, no. 2 (2018): 80. http://dx.doi.org/10.21010/ajtcamv15i2.10.
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Background: Hydnum repandum, Cantharellus cibarius, Ramaria fennica, Boletus edulis, and Craterellus cornucopioides are all wild edible mushroom species from the Kastamonu province. The aim of this study was to investigate antioxidant properties and amino acid contents of these mushrooms.
 Materials and Methods: The mushrooms were analyzed for their free amino acid compositions using a high performance Amino Acid analyzer (Biochrom 30). Also, antioxidant activity and total phenolic concentrations of five different mushroom species were studied by using spectrophotometric methods.
 Results: The mushrooms contained 17 amino acids (g/100 g): Glutamic acid (2.56–1.11), Alanine (1.49–0.54), Arginine (1.62–6.77), Aspartic acid (1.45–0.81), Leucine (1.08–0.64), Methionine (1.05–0.06), Valine (1.05–0.66), Lysine (1.01–0.57), Serine (0.68–0.38), Cysteine (0.88–0.11), Isoleucine (0.61–0.39), Glycine (0.82–0.35), Threonine (0.82–0.44), Phenylalanine (0.66–0.42), Proline (0.60–0.47), Tyrosine (0.58–0.38), and Histidine (0.48–0.22). The total free amino acid (TAA) contents ranged from 169.2 mg/g in Boletus edulis to 89.1 mg/g in Hydnum repandum. These five different mushroom species contain eight essential amino acid species except tryptophan. The antioxidant activity of mushroom extracts was expressed as the percentage of DPPH radical inhibition and IC50 values (mg/mL). The percentage of inhibition ranged from 2.38% to 88.05% and IC50 values ranged from 0.03 to 13.98 mg/mL. The total phenolic content ranged from 0.66 to 7.74 mg/g of dry mushroom extract, expressed as gallic acid equivalents.
 Conclusion: Methanolic extract of Boletus edulis showed the highest phenolic content and strong antioxidant activity. As a result, the significant linear correlation between the values for the total phenolic content and antioxidant activity of mushroom extracts was corroborated in all of the investigated mushroom species, with the exception of Craterellus cornucopioides.
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Iguchi, Kazuaki, Koji Nagashima, Jun Mochizuki, Hiroyuki Yamamoto, Keiko Unno, and Noriyuki Miyoshi. "Enokitake Mushroom and Its Active Component, Adenosine, Which Restores Testosterone Production in Impaired and Fatigued Mouse Models." Nutrients 15, no. 9 (2023): 2142. http://dx.doi.org/10.3390/nu15092142.
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Several studies have reported the effects of the consumption of various mushroom species on the testes in animal experimental models. Mushrooms, including enokitake mushrooms (Flammulina velutipes), and vegetables contain adenosine may affect testosterone production. Here, we aimed to elucidate the effects of enokitake and its active component, adenosine, on testosterone production in primary cultures of testicular cells in vivo using mice models and in vitro. The administration of enokitake ethanolic extract increased testosterone production in the cisplatin-impaired mouse model. The direct effect of mushroom extracts on testicular cells was examined and liquid chromatography–mass spectrometry analysis confirmed that the mushroom- and vegetable-induced increase in testosterone production mainly involved adenosine. Additionally, the administration of enokitake extract or adenosine to wet floor fatigue model mice promoted testicular testosterone production and enhanced Leydig cell function through insulin-like peptide three level upregulation. Structurally related compounds, including cordycepin, showed lower bioactivity than adenosine. This study showed that the ingestion of adenosine-containing mushrooms and vegetables may effectively increase testicular testosterone production. We conclude that mushrooms with a relatively high adenosine content, such as enokitake, may be useful against aging and fatigue.
Dissertations / Theses on the topic "Mushroom extract"
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Zhou, Tianjiao, and 周天骄. "Effect of mushroom extract on endothelial function." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hub.hku.hk/bib/B48334455.
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Hyperglycemia is associated with a higher risk for the development of cardiovascular diseases such as atherosclerosis and hypertension. Hyperglycemia-induced generation of reactive oxygen species and the endothelial dysfunction largely account for this phenomenon. Ergothioneine is a naturally occurring amino acid that is abundantly found in mushroom. Numerous benefits have been found associated with ergothioneine such as cation chelating, regulation of gene expression, improvement in immunity and bioenergetics, and of most concern its antioxidative property.
The aim of this study was to investigate whether mushroom extract and synthetic ergothioneine can exert protective effect on endothelial cells against oxidative stress. Human umbilical vein endothelial cells served as the cell model. Pyrogallol, hydrogen peroxide and high glucose were used to create the oxidative stress condition in endothelial cells. Biochemical assay was used to measure the viability of the cells. It was found that only the mushroom extract could significantly reduce the cell death induced by pyrogallol. Both the mushroom extract and synthetic ergothioneine significantly decreased the cell death induced by high glucose. However, neither mushroom extract nor synthetic ergothioneine have any positive effect on hydrogen peroxide-induced cell death.
These results indicated that mushroom extract and synthetic ergothioneine did exert certain level of protective effect on endothelial cells. However, this protective effect is relatively weak. Besides, it is still unclear if antioxidation is the sole mechanism accounting for the cytoprotective effect of ergothioneine. Further investigation is required to examine if other mechanisms are also involved.<br>published_or_final_version<br>Pharmacology and Pharmacy<br>Master<br>Master of Medical Sciences
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Chen, Yi-Wei, and 陳奕瑋. "Studies on the Effect of Graptopetalum paraguayense extract on the Mushroom Tyrosinase Activity." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/z76wmr.
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碩士<br>靜宜大學<br>應用化學系<br>90<br>Tyrosinase or polyphenol oxidase (EC1.14.18.1) is a copper-containing enzyme that is widespread in nature. It is responsible for not only melanization in animals but also browning in plants. In recent years, there is an increasing interest in finding natural tyrosinase inhibitors from plants and microorganisms. The tyrosinase inhibitors should have broad applications, especially in medicinal and cosmetics products in relation to hyperpigmentation. This study was aimed to evaluate the tyrosinase inhibitory activity of water or 50% ethanolic extracts from Graptopetalum paraguayense. The results were also compared with those obtained from the well-known tyrosinase inhibitors, benzoic acid and kojic acid. The results showed that the water or 50% ethanolic extracts of Graptopetalum paraguayense had tyrosinase inhibitory activity on the L-Dopa oxidation by mushroom tyrosinase. It was also found that the inhibitory activity increased with the concentrations of extracts to a certain extent and then leveled off as the concentration further increased. The 50% ethanolic extracts of Graptopetalum paraguayense had higher tyrosinase inhibitory activity than the water extracts, as evidenced by the lower half-inhibition concentration (IC50) needed for this oxidation. As compared to the well-known tyrosinase inhibitors, the Graptopetalum paraguayense extracts showed less inhibitory activity than benzoic acid and kojic acid. The inhibition was also a function of pH, becoming less as the pH was increased. In addition, the inhibition kinetics analyzed by a Dixon plots found that the Graptopetalum paraguayense extract to be a competitive inhibitor for this oxidation. The results of this study implied that Graptopetalum paraguayense had tyrosinase inhibitory activity.
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"Hypotensive and renal physiological effects of an extract from phoenix mushroom, Pleurotus sajor-caju." Chinese University of Hong Kong, 1986. http://library.cuhk.edu.hk/record=b5885708.
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Shih, Yin Fang, and 石尹芳. "Effects of needle mushroom extract addition on the quality offresh ground pork during cold storage." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/11411741950986331028.
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碩士<br>中國文化大學<br>生活應用科學系<br>101<br>Abstract
The main purpose of this study was to investigate the effect of needle mushroom extract addition on quality of raw ground pork during cold storage. The extracts were prepared with 70% ethanol or hot water. The ethanol (E) or hot water (W) needle mushroom extracts were added respectively in raw ground pork by the concentrations of 0、1、3、5% (v/w). The meat samples were stored at 4±1℃and drawn at 2 days interval (0, 2nd, 4th, 6th, and 8th day) for evaluation of pH, Hunter color values, color difference (ΔE), met-myoglobin formation percentage (Met-Mb%), 2-thiobarbituric acid reacting substances (TBARS) values, and total plate counts. The results indicated that among the ethanol extract treatments, the pork meat samples treated with 5% (E-5) showed a small decrease in the pH value. E-5 samples exhibited higher L (lightness) and a (redness) values significantly (p < 0.05) than the other samples during storage. In the E-5 treatment, slight increases in the Met-Mb% and TBARS values were observed during the storage period, yet, were lower than the control, E-1 and E-3 treated samples (p < 0.05). For the experiments of hot water extract, addition to pork meat at a level of 3% (W-3) was effective to suppress metMb formation as well as lipid oxidation during storage. The antioxidant effects of E-5 and W-3 were further compared to ascorbic acid (AA) and tocopherol (Toc). The data showed that the groups of E-5, W-3 and AA stabilized the pH values compared with the control and Toc groups. Regard to color, E-5 had high values in brightness and yellowness, however, Toc had a high value in redness. During the storage, AA (1.52) showed a minimal ΔE value, followed by Toc (1.75), W-3 (2.08) and E-5 (2.57), which were significantly lower than the control group (4.96) (p < 0.05). The Met-Mb% of Toc (28.38%), E-5 (39.88%), W-3 (40.76%), and AA (45.76%) were significantly lower than control (71.07%) (p < 0.05). The TBARS values (mg/kg) of Toc (2.06), AA (2.31), W-3 (2.44), and E-5 (2.47) were significantly lower than control (2.96) (p < 0.05). The total plate count (log CFU/g) of E-5 (10.10), Toc (10.11) were significantly lower than the W-3 (10.26), AA (10.36) and control (10.44). In conclusion, the raw ground pork added with 5% ethanol or 3% hot water needle mushroom extracts had shown the effects of the stabilization of pH, preservation of color, inhibition of lipid oxidation and met-myoglobin formation effectively at 4℃ storage.
Key words: needle mushroom, extract, antioxidant, ground pork
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Guan, Jen-Sheng, and 官振聖. "Effects of the Brazilian Mushroom Extract and It’s Polysaccharides on Wound Healing After Radiation Injury." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/a727d3.
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碩士<br>慈濟科技大學<br>放射醫學科學研究所<br>106<br>Abstract
Brazilian mushrooms are well-known traditional medicinal mushrooms, and studies have shown that they have certain effects on cellular immunity, anticancer activity, and radiation protection. The purpose of this study is to assess the potential and possible mechanism of Brazilian mushroom extract (BM) and polysaccharide (BMP) on wound healing after radiation injury. In this study, mouse fibroblasts (NIH-3T3) were used to model the model of cancer patients with local irradiation and wound healing after surgery. In wound healing, many factors may lead to impaired wound healing that slows down the rate of repair, delays healing, and even does not heal. The experimental conditions were divided into six groups, including the control group,
Brazilian mushroom extract group, Brazil mushroom polysaccharide group, radiation control group (5 Gy), radiation plus Brazil mushroom extract group, and radiation plus Brazilian mushroom polysaccharide group.
Brazilian mushroom polysaccharides are high-molecular polysaccharides, which can induce many physiological reactions and regulate physiological functions. The results also show that the Brazilian mushroom extract and polysaccharides have the ability to promote wound healing in cells, confirming accelerated healing by ERK and NF-κB. The polysaccharides inhibit the production of ROS after irradiated by cells. At the same time, the polysaccharides also prevented the G2/M phase arrest of fibroblasts in NIH-3T3 mice after radiation injury. Extracts and polysaccharides both reduced apoptosis and increased cell migration rate after radiation injury. From the results, The Brazilian mushroom extract and polysaccharide stimulated ERK and NF-κB secretion in fibroblasts of NIH-3T3 mice to induce cell activation to increase the rate of inflammation, proliferation, and migration of cells to accelerate wound healing. Presumably related to its Ras and MMP-9 pathway.
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HUANG, CHUN-MAN, and 黃春滿. "Effect of extraction method on the distribution patterns of taste and flavfor components in straw-mushroom Volvariella volvacea (Bull, ex Fr.) Sing extract." Thesis, 1990. http://ndltd.ncl.edu.tw/handle/46221247169921534956.
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Yu, Wen-Chun, and 余文鈞. "Molecular mechanism of alcoholic extract from medicinal mushroom Inonotus obliquus improve early-stage nephritic dysfunction and late-stage renal fibrosis of type 2 diabetic mice-derived." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/16841293051496925703.
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碩士<br>南台科技大學<br>生物科技系<br>97<br>In the study, we are looking for construct many kinds of non-genetic deficient DM 2 animal models, which closely simulates the metabolic abnormalities of the human disease and is also cost-effective compared with the genetic models currently available. It is suggested that disease models prior to serving with renal fibrosis, which may be used for studies on type 2 diabetic nephropathy animal models. The experimental mice were orally administrated high-fat diet (HFD) for consecutive 8 weeks, following by 7 consecutive days of streptozotocin (STZ, 50mg/Kg, ip) plus nicotinamide (NA, 200mg/Kg, ip), eventually which almost caused hyperglycemia and hyperinsulina occurred on HFD-controlled C57BL/6 mice (fasting blood glucose over 150 mg/dl, and ACR over 2 g/mg, alternatively).
Inonotus obliquus is a white rot fungus, which belongs to the family Hymenochaetaceae of Basidiomycetes. Recently, there have been reports hat melanin complexes from sclerotia contain strong anti-oxidants. In our study, we detect the effect of hyperglycemia via the toxicity of diabetic nephropathy-prone, which produced by hyperglycemia from the damage of LLC-PK1 renal tubular cells. It suggested that the effectiveness of Inonotus obliquus needed to be conducted for the treatment of type 2 diabetic nephropathy. The preparation of experimental animals started with high fat diet (HFD) administration for 8 weeks followed by intraperitoneal injection of streptozotocin (STZ 50 mg/Kg) and nicotinamide (NA, 200 mg/kg). Once fasting hyperglycemia (over 150mg/dl, fasting period), hyperinsulinemia (10 unit), and positive microalbuminuria (ACR, 2 mg/g Creatinine) occured gradually on HFD-controlled C57BL/6 mice, the candidates were divided into 4 groups, (1) saline, (2) Innonotus oblique (IO), (3) rosiglitazone (R.S), and (4) Cordyceps sinensis (CS). Treatment with oral dosing ethanol extracts of Innonotus oblique and Cordyceps sinensis of 1000 mg/kg/day and 300 mg/kg/day for 8 weeks. The result MTT assay will claim the glucotoxicity, respectively in renal proximal tubular cell line（LLC-PK1） in vitro in a concentration-dependent AGE. We found that the Innonotus oblique also improve the glucose tolerance and insulin resistance with increasing TGF-β and NFκB expression kindey of high fat diet-induced diabetic nephropathy mice by western blotting. Immunohistochemical evaluation showed weak TGF-β staining in the glomeruli of native and I.O group, but in the control group the signal was markedly stronger.
In conclusion, our data demonstrated that the alcoholic extract from Inonotus obliquus treatment has shown a potential anti-hyperglycemic effect, and significantly inhibited the early stage and late-stage renal fibrosis of glucolipotoxicity-induced nephrofibrosis in high fat diet-induced diabetic nephropathy mice.
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Dias, Ana Sofia Couto Bizarro de Castro. "Study of antitumor and immunomodulatory activities of wild mushroom extracts." Master's thesis, 2014. http://hdl.handle.net/1822/34565.
Full textAbstract:
Dissertação de mestrado em Genética Molecular<br>Mushrooms and their compounds are widely appreciated, not only for their nutritional but also for their medicinal properties. In fact, the search for various bioactive properties in different mushroom extracts or in the compounds isolated from those mushroom has been the focus of attention from the scientific community working in the area of natural products. The present work has focused on the study of the antitumor and immunomodulatory properties of extracts from three different mushrooms. Thus, the first aim of the present work was to gain insight into the mechanism of action of a methanolic extract of Cordyceps militaris in the non-small cell lung cancer cell line NCI-H460, since this extract had previously been shown to have tumour cell growth inhibitory activity in this cell line in particular. In addition, the second aim was to study the immunomodulatory activity of the Suillus luteus polysachararidic (PLS) extract and of the Morchella esculenta phenolic extract, using the monocytic THP-1 cell line which differentiates into machrophages upon stimulation.
The response of NCI-H460 cells to the methanolic extract of C. militaris was studied regarding its effect on cellular viability, proliferation, cell cycle profile, apoptosis and DNA damage. Results showed that treatment with the methanolic extract of C. militaris caused a decrease in NCI-H460 cellular proliferation, a cell cycle arrest at G0/G1 and an increase in apoptosis. Interestingly, treatment with the extract was shown to increase the cellular levels of p53 and p21. Moreover, this study also showed evidence of cellular DNA damage caused by this extract, since increased levels of P-H2A.X and 53BP1 foci/cell were observed. Overall, this part of the work suggested that the methanolic extract of C. militaris affected NCI-H460 cellular viability through a mechanism which involved DNA damage and p53 activation.
In addition, preliminary experiments were carried out to gain insight into the immunomodulatory potential of a PLS extract of S. luteus and of a phenolic extract of M. esculenta in THP-1 cells. Results showed that neither of the extracts presented cytotoxicity towards THP-1 cells or induced THP-1 monocytes differentiation into macrophages. Interestingly, the PLS extract of S. luteus caused a dose-dependent increase in the metabolic activity of THP-1 monocytes, probably due to increased proliferation. These preliminary results need be further confirmed and continued in future work.
Overall, the work carried out in this thesis further supports the potential of mushrooms extracts in the search for bioactive compounds.<br>Os cogumelos e seus compostos são muito apreciados, não só pelas suas propriedades nutricionais, mas também pelas medicinais. De facto, a procura de propriedades bioativas em diferentes extratos de cogumelos ou em compostos isolados desses cogumelos, tem sido um foco de interesse da comunidade científica que trabalha na área de produtos naturais. O presente trabalho visou o estudo de propriedades antitumorais e imunomoduladoras de extratos de três cogumelos diferentes. Assim sendo, como primeiro objetivo deste trabalho pretendeu-se analisar o mecanismo de ação de um extrato metanólico de Cordyceps militaris na linha celular NCI-H460 (de cancro do pulmão de não pequenas células), uma vez que tinha sido previamente demonstrado que este extrato inibe o crescimento celular, destas células em particular. Além deste, um segundo objetivo visou o estudo da atividade imunomoduladora de um extrato polisacarídico (PLS) de Suillus luteus e de um fenólico de Morchella esculenta, usando a linha celular monocítica THP-1 que se diferencia em macrófagos, após estimulação.
Foi estudada a resposta das células NCI-H460 ao tratamento com o extrato metanólico de C. militaris relativamente ao efeito na viabilidade celular, proliferação, perfil do ciclo celular, apoptose e no dano no DNA. Os resultados demonstraram que o tratamento com o extrato metanólico de C. militaris nas células NCI-H460 diminuiu a proliferação celular, bloqueou o ciclo celular nas fases G0/G1 e induziu apoptose. De particular interesse foi o facto de este extrato causar um aumento dos níveis de p53 e p21. Para além disso, este estudo também mostrou ainda evidências de danos no DNA causados por este extrato, dada a observação de aumento quer nos níveis de P-H2A.X como no número de 53BP1 foci/célula. Em geral, esta parte do trabalho sugeriu que o extrato metanólico de C. militaris diminuiu a viabilidade celular das células NCI-H460 por um mecanismo que envolve a ativação de danos no DNA e de p53.
Neste trabalho, foram realizadas experiências preliminares para averiguar o potencial imunomodulador de um extrato PLS de S. luteus e de um extrato fenólico de M. esculenta em células THP-1.
Os resultados demonstraram que nenhum dos extratos analisados apresentou citotoxicidade para células THP-1 ou induziu a diferenciação desta linha celular de monócitos em macrófagos. De particular interesse foi o facto de se verificar que o extrato PLS do S. luteus causou um aumento (dependente da dose) da atividade metabólica de monócitos THP-1, provavelmente devido ao aumento da proliferação celular. Estes resultados preliminares terão ainda de ser confirmados num trabalho futuro, assim como o potencial imunomodulador destes extratos.
De um modo geral, o trabalho realizado nesta tese contribui para a descrição do potencial dos extratos de cogumelos na busca de compostos bioativos.
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Chen, pei-ting, and 陳姵廷. "The antioxidative properties of solvent extracts from mushrooms." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/73793322639592355523.
Full textAbstract:
碩士<br>中國文化大學<br>生活應用科學系<br>100<br>The study is designed to explore the antioxidant properties of mushroom extracts and the effects of extraction solvents; in addition, to investigate the contents and profiles of total phenolics, including both soluble free and bound forms, by applying solvent extraction, alkaline hydrolysis, and liquid-liquid extraction methods. Five mushrooms (Hypsizygus marmoreu, Flammulina velutipes collybia velutipes , Pleurotus eryngii, Pleurotus citrinopileatus, Lentinus edodes) were investigated and five antioxidant capacities (DPPH scavenging activity, reducing power, ferrous chelating effect, superoxide anion scavenging activity and trolox equivalent antioxidant capacity (TEAC)) were measured. The results showed significant differences (p < 0.05) in the chemical compositions of the samples. Among five antioxidant capacities, mushroom extracts possessed only DPPH scavenging activity, reducing power, and TEAC, which activities were related to the varieties of mushroom. To these three antioxidant activities, Pleurotus citrinopileatus and Pleurotus eryngii were the highest. Pleurotus citrinopileatus had the highest total phenolic content (7.36 mg gallic acid equiv/g), followed by Pleurotus eryngii, Lentinus edodes, Hypsizygus marmoreu and Flammulina velutipes Collybia velutipes. The major portion of phenolics in mushrooms existed in the free form (51% in Hypsizygus marmoreu, 75% in Flammulina velutipes collybia velutipes, 70% in Pleurotus eryngii, 56% Pleurotus citrinopileatus, 69% in Lentinus edodes). The higher content of total phenolics and antioxidant activity were obtained from extraction with 80% acetone with comparision to 100% methanol.
Key words: mushrooms, antioxidant, phenolics
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"In vitro antioxidant and anti-angiogenic effects of mushroom water extracts." 2011. http://library.cuhk.edu.hk/record=b5894512.
Full textAbstract:
Lai, Tsz Ching.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2011.<br>Includes bibliographical references (leaves 121-136).<br>Abstracts in English and Chinese.<br>Acknowledgements<br>Abstract<br>摘要<br>Content<br>List of tables<br>List of figures<br>List of abbreviations<br>Chapter Chapter 1: --- Introduction --- p.1<br>Chapter 1.1 --- Introduction of food market trends in Hong Kong and mushroom productivity in the world --- p.1<br>Chapter 1.1.1 --- Agrocybe aegerita --- p.1<br>Chapter 1.1.2 --- Pleurotus spp --- p.2<br>Chapter 1.1.3 --- Pholiota nameko --- p.3<br>Chapter 1.2 --- Objectives --- p.5<br>Chapter Chapter 2: --- Chemical assays for in vitro antioxidative properties of mushroom extracts --- p.6<br>Chapter 2.1 --- Introduction --- p.6<br>Chapter 2.1.1 --- Reactive oxygen species (ROS) --- p.6<br>Chapter 2.1.1.1 --- Definition of ROS --- p.6<br>Chapter 2.1.1.2 --- Sources of ROS --- p.6<br>Chapter 2.1.1.2.1 --- Endogenous sources of ROS --- p.6<br>Chapter 2.1.1.2.2 --- Exogenous sources of ROS --- p.8<br>Chapter 2.1.1.3 --- Damaging effects of ROS --- p.8<br>Chapter 2.1.2 --- Antioxidants --- p.10<br>Chapter 2.1.2.1 --- Mechanism of action --- p.10<br>Chapter 2.1.2.2 --- Sources of antioxidants --- p.11<br>Chapter 2.1.2.2.1 --- Dietary antioxidants --- p.11<br>Chapter 2.1.2.2.2 --- Antioxidants in edible mushrooms --- p.12<br>Chapter 2.1.2.2.3 --- Phenolic compounds in mushrooms --- p.13<br>Chapter 2.2 --- Materials and Methods --- p.16<br>Chapter 2.2.1 --- Materials --- p.16<br>Chapter 2.2.1.1 --- Mushroom fruiting bodies --- p.16<br>Chapter 2.2.2 --- Principles of Methods and Experimental Protocols --- p.17<br>Chapter 2.2.2.1 --- Sample preparation --- p.17<br>Chapter 2.2.2.2 --- Evaluation of antioxidant capacity --- p.18<br>Chapter 2.2.2.2.1 --- DPPH radical scavenging activity --- p.18<br>Chapter 2.2.2.2.2 --- Superoxide anion scavenging activity --- p.19<br>Chapter 2.2.2.2.3 --- Hydroxyl radical scavenging activity --- p.20<br>Chapter 2.2.2.2.4 --- Hydrogen peroxide scavenging activity --- p.22<br>Chapter 2.2.2.3 --- Determination of phenolic compounds --- p.24<br>Chapter 2.2.2.3.1 --- Total phenolic content --- p.24<br>Chapter 2.2.2.3.2 --- Identification of phenolic acids --- p.25<br>Chapter 2.2.3 --- Statistical analysis --- p.27<br>Chapter 2.3 --- Results and Discussion --- p.28<br>Chapter 2.3.1 --- Extraction yield --- p.28<br>Chapter 2.3.2 --- Evaluation of antioxidant capacity --- p.29<br>Chapter 2.3.2.1 --- DPPH radical scavenging activity --- p.29<br>Chapter 2.3.2.2 --- Superoxide anion scavenging activity --- p.31<br>Chapter 2.3.2.3 --- Hydroxyl radical scavenging activity --- p.33<br>Chapter 2.3.2.4 --- Hydrogen peroxide scavenging activity --- p.35<br>Chapter 2.3.2.5 --- Comparison of the effective concentrations (EC50) of mushroom water extracts in different antioxidant assays --- p.37<br>Chapter 2.3.3 --- Determination of phenolic compounds --- p.38<br>Chapter 2.3.3.1 --- Total phenolic content --- p.38<br>Chapter 2.3.3.2 --- Identification of phenolic acids --- p.39<br>Chapter 2.4 --- Summary --- p.45<br>Chapter Chapter 3: --- Anti-angiogenic properties of the Aa water extract --- p.46<br>Chapter 3.1 --- Introduction --- p.46<br>Chapter 3.1.1 --- Angiogenesis --- p.46<br>Chapter 3.1.1.1 --- Process of angiogenesis --- p.46<br>Chapter 3.1.1.2 --- Regulations of angiogenesis --- p.47<br>Chapter 3.1.1.2.1 --- Fibroblast growth factor (bFGF) --- p.47<br>Chapter 3.1.1.2.2 --- Vascular endothelial growth factor (VEGF) --- p.48<br>Chapter 3.1.2 --- Tumor angiogenesis --- p.49<br>Chapter 3.1.2.1 --- ROS generation in tumor cells --- p.50<br>Chapter 3.1.2.2 --- Hydrogen peroxide and VEGF --- p.51<br>Chapter 3.1.2.3 --- Previous studies on tumor angiogenesis --- p.52<br>Chapter 3.1.2.3.1 --- ROS and endothelial cells proliferation --- p.52<br>Chapter 3.1.2.3.2 --- VEGF and endothelial cells functions --- p.53<br>Chapter 3.1.3 --- Use of antioxidants in cancer treatment --- p.53<br>Chapter 3.1.3.1 --- Antioxidant use of cancer therapy --- p.53<br>Chapter 3.1.3.2 --- Antioxidant and endothelial cells functions --- p.54<br>Chapter 3.1.3.3 --- Anti-angiogenic effects of polyphenols --- p.56<br>Chapter 3.1.3.3.1 --- Phenolic acids --- p.56<br>Chapter 3.1.3.3.2 --- Tea catechin --- p.57<br>Chapter 3.1.3.3.3 --- Resveratrol --- p.57<br>Chapter 3.1.3.3.4 --- Genistein --- p.58<br>Chapter 3.2 --- Principles of Methods and Experimental Protocols --- p.60<br>Chapter 3.2.1 --- Sample preparation --- p.60<br>Chapter 3.2.2 --- Toxicity of the Aa water extract --- p.60<br>Chapter 3.2.2.1 --- Limulus amebocyte lysate (LAL) test --- p.60<br>Chapter 3.2.2.2 --- Toxicity towards normal cells --- p.61<br>Chapter 3.2.2.2.1 --- Cell line and its subculture --- p.61<br>Chapter 3.2.2.2.2 --- Colorimetric (MTT) assay --- p.62<br>Chapter 3.2.3 --- Effect of the Aa water extract on cancer cells --- p.63<br>Chapter 3.2.3.1 --- Cell line and its subculture --- p.63<br>Chapter 3.2.3.2 --- Redox status --- p.63<br>Chapter 3.2.3.3 --- VEGF secretion --- p.65<br>Chapter 3.2.4 --- In vitro cell culture anti-angioenesis analysis --- p.66<br>Chapter 3.2.4.1 --- Cell line and its subculture --- p.66<br>Chapter 3.2.4.2 --- Endothelial cells proliferation --- p.67<br>Chapter 3.2.4.3 --- Endothelial cells migration --- p.68<br>Chapter 3.2.4.3.1 --- Wound healing assay --- p.68<br>Chapter 3.2.4.3.2 --- Transwell culture insert assay --- p.69<br>Chapter 3.2.4.4 --- Endothelial cells tubule formation --- p.71<br>Chapter 3.2.5 --- In vitro organ culture anti-angiogenesis analysis --- p.72<br>Chapter 3.2.5.1 --- Aortic ring assay --- p.72<br>Chapter 3.2.6 --- Statistical analysis --- p.74<br>Chapter 3.3 --- Results and Discussions --- p.75<br>Chapter 3.3.1 --- Toxicity of the Aa water extract --- p.75<br>Chapter 3.3.1.1 --- Limulus amebocyte lysate (LAL) test --- p.75<br>Chapter 3.3.1.2 --- Toxicity towards normal cells --- p.75<br>Chapter 3.3.2 --- Effect of the Aa water extract on cancer cells --- p.77<br>Chapter 3.3.2.1 --- Redox status --- p.77<br>Chapter 3.3.2.2 --- VEGF secretion --- p.79<br>Chapter 3.3.2.3 --- Relationship between intracellular ROS and VEGF secretion detected --- p.80<br>Chapter 3.3.3 --- Effect of the Aa water extract on angiogenesis --- p.82<br>Chapter 3.3.3.1 --- Endothelial cells proliferation --- p.82<br>Chapter 3.3.3.2 --- Endothelial cells migration --- p.84<br>Chapter 3.3.3.2.1 --- Wound healing assay --- p.84<br>Chapter 3.3.3.2.2 --- Transwell culture insert assay --- p.87<br>Chapter 3.3.3.3 --- Endothelial cells tubule formation --- p.90<br>Chapter 3.3.3.4 --- Aortic ring assay --- p.97<br>Chapter 3.3.4 --- Effect of phenolic acids on endothelial cells --- p.101<br>Chapter 3.3.4.1 --- Endothelial cells proliferation --- p.101<br>Chapter 3.3.4.2 --- Endothelial cells migration --- p.102<br>Chapter 3.3.4.2.1 --- Wound healing assay --- p.102<br>Chapter 3.3.4.2.2 --- Transwell culture insert assay --- p.105<br>Chapter 3.3.4.3 --- Endothelial cells tubule formation --- p.106<br>Chapter 3.3.4.4 --- Aortic ring assay --- p.112<br>Chapter 3.4 --- Summary --- p.116<br>Chapter Chapter 4 --- Conclusions and future works --- p.118<br>References --- p.121
Books on the topic "Mushroom extract"
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Easy Guide to Making Mushroom Tintures: The Step by Step Guіdе оn Hоw Tо Extract Tіnсturеѕ, Uѕеѕ, Benefits аnd dоѕаgеѕ Tо Cure. Independently Published, 2020.
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HOLMES, Daniels. Making Your Mushroom Tincture: Easy Guide on Hоw Tо Mаkе Mushroom Tinctures, Extracts, Planting ,Usages and Lots More. Independently Published, 2020.
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Cook, Jeffery. Complete Guide on Making Mushroom Tinctures: The Essential Guіdе оn Hоw Tо Extract Tіnсturеѕ, Uѕеѕ, Benefits аnd dоѕаgеѕ Tо Cure Vаrіоuѕ Aіlmеntѕ. Independently Published, 2020.
Find full textBook chapters on the topic "Mushroom extract"
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Hsieh, Tze-Chen, Hsiao Hsiang Chao, Yang Chu, Barbara B. Doonan, and Joseph M. Wu. "Aqueous and Ethanolic Extracts of Medicinal Mushroom Trametes versicolor Interact with DNA: A Novel Genoactive Effect Contributing to Its Antiproliferative Activity in Cancer Cells." In Medicinal Mushrooms. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6382-5_7.
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Revolidis, Ioannis, and Alan Dahi. "The Peculiar Case of the Mushroom Picking Robot: Extra-contractual Liability in Robotics." In Robotics, AI and the Future of Law. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2874-9_3.
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Herrera, Erika Murgueitio, Sofía Lucio, Andrés Izquierdo, Geovanna Arroyo, Carina Stael, and Theofilos Toulkeridis. "Application of Multicomponent Nanoparticles Synthesized with Andean Blackberry Fruit Extract (Rubus Glaucus) NPs (Ca, Fe, Mg-Rg) for Oyster Mushrooms (Pleurotus Ostreatus)." In Emerging Research in Intelligent Systems. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-52258-1_11.
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Bains, Aarti, Dipsha Narang, Prince Chawla, and Sanju Bala Dhull. "Mushroom Extract–Reduced Metal Nanoparticles." In Nanotechnological Approaches in Food Microbiology. CRC Press, 2020. http://dx.doi.org/10.1201/9780429342776-2.
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Tiwari Pandey, Aprajita, Ishan Pandey, Prashasti Tripathi, Vartika Mishra, Priyanka Yadav, and Mohan Prasad Singh. "Antimicrobial Potential of Mushrooms: Emergence of Mycotherapy." In Mushrooms: A Wealth of Nutraceuticals and An Agent of Bioremediation. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815080568123010008.
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The 21st century enters a “post-antibiotic world,” with just a few alternatives for combating antibiotic-resistant microbial strains. The search for new antimicrobials which could overcome the situation of antimicrobial resistance is of prime importance and the need of hour. Herbal antimicrobials pose a plethora of new antimicrobial drug discoveries. Mushrooms give rise to a number of bioactive chemicals that are known to have anti-pathogenic qualities as well as safer and more effective therapeutic effects in the treatment of human diseases. As a result, this chapter emphasises that mycoconstituents might be an alternate treatment regimen and could play a role in novel drug discovery against various infections. This chapter contains information about the potential use of mycoconstituents in the management and treatment of infectious diseases. The approaches illustrate the importance of primary screening of bioactive molecules from mushrooms as a potential step by offering new possibilities for clinical and pharmacological research and development. Traditional antibiotics can now be substituted with newer and more effective natural antimicrobials derived from mushroom extracts to treat a variety of fatal and multi-drug resistant infectious diseases. As a result, this functional food extract might play a significant role in naturally combating infections and offering a comprehensive approach to treatments. Finally, we will go through the mechanics of mushroom antimicrobial potentials and presents an insight into Mycotherapy.
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Ameer, Kashif, Maruf Ahmed, Mian Anjum Murtaza, and Shahida Anusha Siddiqui. "Lentinula edodes (Berk.) Pegler." In Phytochemistry and Nutritional Composition of Significant Wild Medicinal and Edible Mushrooms. Royal Society of Chemistry, 2023. http://dx.doi.org/10.1039/bk9781837672097-00242.
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Lentinula edodes (Berk.) Pegler (shiitake) is an edible mushroom originating from East Asia that is widely cultivated and consumed globally. Medicinal and edible mushrooms have been exploited for centuries in traditional and alternative medicines and oriental therapies to treat ailments. Contemporary research findings have endorsed the health benefits of edible mushrooms. Modern clinical practices in Korea, Japan, and China rely on mushroom-derived extracts and supplements. Mushrooms have been researched for their phytochemistry, nutritional, and medicinal properties. In terms of medicinal effects, mushrooms are consumed by health-conscious consumers worldwide for cancer prevention, combating cardiovascular diseases, blood circulation improvement, immunomodulatory effects, and cholesterol-lowering properties. Some medicinal mushrooms, such as the shiitake mushroom, have been utilized for centuries to treat emotional and physical stress, chronic hepatitis, and gastric ulcers. Shiitake mushrooms exhibit antioxidant, anti-inflammatory, antibacterial, antifungal, anticarcinogenic and immunomodulatory activities. This chapter details the therapeutic benefits of shiitake mushrooms. Important bio-components in shiitake mushrooms include vitamin E, butyric acid, ergosterol, and oleic and linoleic acids, which make shiitake mushrooms an important anti-atherosclerotic agent. These therapeutic benefits have boosted the popularity of shiitake mushrooms as a functional food.
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Rahimah, S. B., Y. Kharisma, M. K. Dewi, J. Hartati, and W. Maharani. "Acute toxicity test for the ethanolic extract of the white oyster mushroom." In Medical Technology and Environmental Health. CRC Press, 2020. http://dx.doi.org/10.1201/9781003016700-9.
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Parkhach, Marharyta Evgenievna, Alexandra Valeryevna Knyazeva, and Evgeny Dmitrievich Antonenko. "JUSTIFICATION OF THE CHOICE OF EXCIPIENTS FOR OINTMENT WITH GANODERMA APPLANATUM MUSHROOM EXTRACT." In JUSTIFICATION OF THE CHOICE OF EXCIPIENTS FOR OINTMENT WITH GANODERMA APPLANATUM MUSHROOM EXTRACT. ICSP "NEW SCIENCE", 2025. https://doi.org/10.46916/13062025-978-5-00215-820-1.
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"In Vitro Assessment of Shiitake Mushroom (Lentinula edodes) Extract for its Antigingivitis Activity." In Biofilm Control and Antimicrobial Agents. Apple Academic Press, 2014. http://dx.doi.org/10.1201/b16593-15.
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"An Extract of the Medicinal Mushroom Agaricus blazei Murill can Protect Against Allergy." In Recent Advances in Plant-Based, Traditional, and Natural Medicines. Apple Academic Press, 2014. http://dx.doi.org/10.1201/b16611-10.
Full textConference papers on the topic "Mushroom extract"
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Popa, Oana, and Ovidiu Tita. "EVALUATION OF THE PROPERTIES OF YOGURT ENRICHED WITH CRUDE POLYSACCHARIDES OBTAINED FROM DIFFERENT MUSHROOMS." In SGEM International Multidisciplinary Scientific GeoConference 24. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/6.1/s25.10.
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With the development of food production technologies, the consumers attention has turned to products with specific nutritional and health-promoting properties. For this necessity, today's manufacturers have turned their attention to fortified foods, and a raw material that is found in great diversity and that can be used to enrich food is represented by mushrooms. The purpose of this study consists in determining how the addition of different concentrations of crued polysaccharides extracted from mushrooms and added to milk to obtain new varieties of yogurts can influence its quality. The two mushroom species from which polysaccharides have been extracted are Champignon and Reishi. The obtained analysis showed that the crued polysaccharides extracted from mushrooms consist in carbohydrates (~6% - Champignon mushrooms; ~30% - Reishi mushrooms), proteins (~6.2% - Champignon mushrooms; ~12.6% - Reishi mushrooms) and in a smaller amount in phenolic compounds (~ 0.5% - Champignon mushrooms; ~8% - Reishi mushrooms). To obtain the fortified yogurts, the polysaccharides were added to cow's milk at different concentrations of 0.3% and 0.5% from each species of mushroom. A control sample represented by plain milk that does not contain polysaccharides was also developed. All samples were fermented using commercially available starter cultures. Following the determinations, it was found that the addition of polysaccharides led to a decrease in pH in the case of both types of samples with the addition of polysaccharides from mushrooms, compared to the control sample. The largest decreases in pH were recorded for concentrations of 0.5% added polysaccharides. In the case of syneresis, significant increases were recorded for the samples enriched with polysaccharides, especially for the samples to which concentrations of 0.5% polysaccharides were added (38.02g for the sample with Reishi polysaccharides and 42.22g for the sample with Champignon polysaccharides). The results on the antioxidant activity following the determinations performed recorded the lowest values for the control sample (355?M). The results showed that crude polysaccharides extracted from the two species of mushrooms added to milk before fermentation can improve its properties, but can also affect them, if the concentrations are not exceeded and are not kept under observation.
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Todorović, Jovana D., Aleksandra D. Vesić, Nevena N. Petrović, and Marijana M. Kosanić. "Antimicrobial potential of mushrooms Macrolepiota procera and Chlorophyllum rhacodes." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.304t.
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The aim of this study is to investigate the in vitro antimicrobial properties of the acetone extracts of the mushrooms Macrolepiota procera and Chlorophyllum rhacodes. The microdilution method was used to determine the minimum inhibitory concentration (MIC) and assess the antimicrobial effects against three bacterial and ten fungal species. Overall, the tested mushroom extracts had relatively strong antimicrobial activity against the tested microorganisms. The minimum inhibitory concentration for both extracts related to the tested bacteria and fungi was 0.625 – 20 mg/mL. Extract of C. rhacodes exhibited more powerful antimicrobial properties, with ranged MIC values from 0.625 mg/mL to 10 mg/mL. The Acetone extract of C. rhacodes has shown the most antibacterial activity against S. aureus, while the extract of M. procera has not shown activity against S. aureus as against G. candidum. In comparison to the standard antibiotics as positive controls, the antimicrobial activity of studied extracts was less expressed. The results suggest that mushroom species may be used for pharmaceutical purposes in treating various diseases.
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Todorović, Jovana D., Aleksandra D. Vesić, Nevena N. Petrović, and Marijana M. Kosanić. "Edible mushrooms as promising antioxidants." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.300t.
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Antioxidant activity of the acetone extracts of the mushrooms Macrolepiota procera and Chlorophyllum rhacodes has been screened in vitro by using different methods (DPPH radical scavenging and reducing power assay), and also it has been determined the total phenolic compounds as total flavonoid content. The research findings indicate that the acetone extract of M. procera demonstrated superior antioxidant activities when compared to C. rhacodes. Similarly, the mushroom M. procera displayed a more pronounced effect on reducing power. In addition, the total content of phenol and flavonoid in extracts were determined as pyrocatechol equivalent, and as rutin equivalent, respectively. A higher content of total phenols and flavonoids was detected in the extract of M. procera. The strong relationships between total phenolic and flavonoid contents and the antioxidative activities of tested extracts imply that these compounds have a significant impact on antioxidant activity. The present study highlights that the tested mushroom species exhibit potent antioxidant properties and can be regarded as valuable sources of natural antioxidants.
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Stojanova, Monika, Dragutin Djukic, Marina Todor Stojanova, Aziz Şatana, and Blazo Lalevic. "DETERMINATION OF ANTIBACTERIAL POTENTIAL OF AGARICUS MACROSPORUS AND RUSSULA VESCA MUSHROOM EXTRACTS." In XXVII savetovanje o biotehnologiji. University of Kragujevac, Faculty of Agronomy, 2022. http://dx.doi.org/10.46793/sbt27.477s.
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The aim of this research was to determine the antibacterial potential ofaqueous and ethanolic extract of wild mushroom species: Agaricus macrosporus and Russulavesca. Extracts from R.vesca were characterized with higher values for total carbohydrates and total proteins. Both aqueous extracts had higher antibacterial activity compared to ethanolic extracts. Aqueous extract from R.vesca showed higher antibacterial activity against B. cereus (13.6mm), E.faecalis (12.1 mm), E. coli(16.7 mm) and P.aeruginosa (10.5 mm) compared to gentamicin or neomycin. This study represents a novel starting point for future researchin which mushroom extracts can be used in various industry fields.
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Salleh, N. H. Mohd, Z. Zakaria, N. H. Aziz, A. A. N. Gunny, A. R. Mohamed, and T. Munusamy. "Inhibition of Aspergillus flavus by Momordica charantia ethanolic leaves extract for mushroom protection." In THE PROCEEDING OF THE 1ST INTERNATIONAL CONFERENCE OF CHEMICAL SCIENCE, ENGINEERING AND TECHNOLOGY. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0117927.
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Matić, Jovana, Nevena Petrović, and Marijana Kosanić. "ANTIMICROBIAL AND ANTIOXIDANT PROPERTIES OF THE METHANOLIC EXTRACT OF THE EDIBLE MUSHROOM XEROCOMUS SUBTOMENTOSUS." In 3rd International Symposium on Biotechnology. University of Kragujevac, Faculty of Agronomy in Čačak, 2025. https://doi.org/10.46793/sbt30.72jm.
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This study investigates the antimicrobial and antioxidant potential of methanolic extract of mushroom Xerocomus subtomentosus. The antimicrobial potential was assessed by microdilution method (MIC values ranged from 5 to 40 mg/mL). Antioxidant activity was evaluated using DPPH, ABTS and reducing power assays, alongside total phenol and flavonoid content measurements. The extract demonstrated strong antioxidant potential (IC50: 80.842 μg/mL for DPPH, 6.587 μg/mL for ABTS, while absorbance for reducing power ranged from 0.380 to 1.168). Total phenolics and flavonoids were 40.439 μg PE/mg and 3.899 μg RE/mg. This study confirms that X. subtomentosus is a valuable source of natural antioxidants.
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Perera, TVRC, K. Pakeerathan, and A. Nirosha. "ECO-FRIENDLY MANAGEMENT COMMON LAB CONTAMINANT Trichoderma spp IN OYSTER MUSHROOM PRODUCTION USING AGROBASED INDUSTRY’S BY-PRODUCTS." In The 5th International Conference on Climate Change 2021 – (ICCC 2021). The International Institute of Knowledge Management, 2021. http://dx.doi.org/10.17501/2513258x.2021.5105.
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An abundant supply of low-cost substrate and management of green mold disease-causing fungus Trichoderma are the major hurdles in successful mushroom production. This study aimed to identify the best Agro-based industry’s by-products as a substitute for oyster mushroom production (Pleurotus ostreatus) while managing fungal contaminants eco-friendly. Two sets of In-Vitro [containing 20% extracts, from agro-based industries, such as coffee waste powder, tea dust and Mahua oil cake] and In-Vivo experiments [four substrates such as paddy straw, wood sawdust, paddy husk and banana leaves were incorporated with coffee powder, tea dust and Mahua oil cake] were prepared separately. All the experiments were conducted using a complete randomized design with three replicates. The In-Vitro data [mycelial growth and sporulation of both fungi], In-Vivo data [mycelial mushroom run, pinhead formation and yield] were subjected to ANOVA and DMRT mean separation using SAS 9.1 statistical package at P <0.05. In-Vitro results showed that the Trichoderma mycelial growth was significantly minimum in Mahua (2.5 cM) and coffee (3.6 cM) in comparison to control, whereas, with decreasing concentration of coffee, tea, and Mahua extract P. ostreatus showed enhanced growth. Trichoderma sporulation had significantly affected coffee treatment, and even not sporulate in Mahua treated plants. The In-Vivo experiment proved that spawn run was consistent and significant among the treatments when mixed tea (20 days) and coffee (21 days), respectively, at P <0.05. Treatment wise coffee treated spawn bags took an average of 32.5 days, whereas, in tea-treated substrates, it was more than 36 days to form pinhead. Mahua treated trials showed poor spawn run in all substrates, longer days of pinhead formation, and lower yield. In contrast, the paddy straw + coffee treatment produced a significantly highest yield of 200.67g. When sawdust was the substrate, the addition of tea showed a significantly higher yield of 185.00g than coffee (145.00g). In conclusion, coffee and tea extracts have a significant effect on yield with paddy straw and sawdust while minimizing the growth of Trichoderma. Keywords: Pleurotus ostreatus, eco-friendly, plant extract, substrate, coffee, paddy straw
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Mallikarjuns, K., K. Venkateswara Rao, N. John Sushma, L. Manoj, G. Narasimha, and B. Deva Prasad Raju. "Synthesis and spectroscopic characterization of palladium nanoparticles by using broth of edible mushroom extract." In International Conference on Nanoscience, Engineering and Technology (ICONSET 2011). IEEE, 2011. http://dx.doi.org/10.1109/iconset.2011.6168045.
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Soares, Raquel, Manuela Meireles, Diego Obiol, et al. "Abstract 3570: Maitake mushroom extract induces apoptosis in breast cancer MCF7 cells through BAK1 activation." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-3570.
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Sultan, Safa. "The antifungal activity of aqueous and alcoholic extract of mushroom (Agaricus bisporus) against Aspergillus flavus." In Proceedings of the 1st International Multi-Disciplinary Conference Theme: Sustainable Development and Smart Planning, IMDC-SDSP 2020, Cyperspace, 28-30 June 2020. EAI, 2020. http://dx.doi.org/10.4108/eai.28-6-2020.2298228.
Full textReports on the topic "Mushroom extract"
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Schwartz, Bertha, Vaclav Vetvicka, Ofer Danai, and Yitzhak Hadar. Increasing the value of mushrooms as functional foods: induction of alpha and beta glucan content via novel cultivation methods. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600033.bard.
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During the granting period, we performed the following projects: Firstly, we differentially measured glucan content in several pleurotus mushroom strains. Mushroom polysaccharides are edible polymers that have numerous reported biological functions; the most common effects are attributed to β-glucans. In recent years, it became apparent that the less abundant α-glucans also possess potent effects in various health conditions. In our first study, we explored several Pleurotus species for their total, β and α-glucan content. Pleurotuseryngii was found to have the highest total glucan concentrations and the highest α-glucans proportion. We also found that the stalks (stipe) of the fruit body contained higher glucan content then the caps (pileus). Since mushrooms respond markedly to changes in environmental and growth conditions, we developed cultivation methods aiming to increase the levels of α and β-glucans. Using olive mill solid waste (OMSW) from three-phase olive mills in the cultivation substrate. We were able to enrich the levels mainly of α-glucans. Maximal total glucan concentrations were enhanced up to twice when the growth substrate contained 80% of OMSW compared to no OMSW. Taking together this study demonstrate that Pleurotuseryngii can serve as a potential rich source of glucans for nutritional and medicinal applications and that glucan content in mushroom fruiting bodies can be further enriched by applying OMSW into the cultivation substrate. We then compared the immune-modulating activity of glucans extracted from P. ostreatus and P. eryngii on phagocytosis of peripheral blood neutrophils, and superoxide release from HL-60 cells. The results suggest that the anti-inflammatory properties of these glucans are partially mediated through modulation of neutrophileffector functions (P. eryngiiwas more effective). Additionally, both glucans dose-dependently competed for the anti-Dectin-1 and anti-CR3 antibody binding. We then tested the putative anti-inflammatory effects of the extracted glucans in inflammatory bowel disease (IBD) using the dextran sulfate sodium (DSS)–induced model in mice. The clinical symptoms of IBD were efficiently relieved by the treatment with two different doses of the glucan from both fungi. Glucan fractions, from either P. ostreatus or P. eryngii, markedly prevented TNF-α mediated inflammation in the DSS–induced inflamed intestine. These results suggest that there are variations in glucan preparations from different fungi in their anti-inflammatory ability. In our next study, we tested the effect of glucans on lipopolysaccharide (LPS)-induced production of TNF-α. We demonstrated that glucan extracts are more effective than mill mushroom preparations. Additionally, the effectiveness of stalk-derived glucans were slightly more pronounced than of caps. Cap and stalk glucans from mill or isolated glucan competed dose-dependently with anti-Dectin-and anti-CR-3 antibodies, indicating that they contain β-glucans recognized by these receptors. Using the dextran sulfate sodium (DSS)-inflammatory bowel disease mice model, intestinal inflammatory response to the mill preparations was measured and compared to extracted glucan fractions from caps and stalks. We found that mill and glucan extracts were very effective in downregulatingIFN-γ and MIP-2 levels and that stalk-derived preparations were more effective than from caps. The tested glucans were equally effective in regulating the number of CD14/CD16 monocytes and upregulating the levels of fecal-released IgA to almost normal levels. In conclusion, the most effective glucans in ameliorating some IBD-inflammatory associated symptoms induced by DSS treatment in mice were glucan extracts prepared from the stalk of P. eryngii. These spatial distinctions may be helpful in selecting more effective specific anti-inflammatory mushrooms-derived glucans. We additionally tested the effect of glucans on lipopolysaccharide-induced production of TNF-α, which demonstrated stalk-derived glucans were more effective than of caps-derived glucans. Isolated glucans competed with anti-Dectin-1 and anti-CR3 antibodies, indicating that they contain β-glucans recognized by these receptors. In conclusion, the most effective glucans in ameliorating IBD-associated symptoms induced by DSS treatment in mice were glucan extracts prepared from the stalk of P. eryngii grown at higher concentrations of OMSW. We conclude that these stress-induced growing conditions may be helpful in selecting more effective glucans derived from edible mushrooms. Based on the findings that we could enhance glucan content in Pleurotuseryngii following cultivation of the mushrooms on a substrate containing different concentrations of olive mill solid waste (OMSW) and that these changes are directly related to the content of OMSW in the growing substrate we tested the extracted glucans in several models. Using dextran sulfate sodium (DSS)–inflammatory bowel disease (IBD) mice model, we measured the colonic inflammatory response to the different glucan preparations. We found that the histology damaging score (HDS) resulting from DSS treatment reach a value of 11.8 ± 2.3 were efficiently downregulated by treatment with the fungal extracted glucans, glucans extracted from stalks cultivated at 20% OMSWdownregulated to a HDS value of 6.4 ± 0.5 and at 80% OMSW showed the strongest effects (5.5 ± 0.6). Similar downregulatory effects were obtained for expression of various intestinal cytokines. All tested glucans were equally effective in regulating the number of CD14/CD16 monocytes from 18.2 ± 2.7 % for DSS to 6.4 ± 2.0 for DSS +glucans extracted from stalks cultivated at 50% OMSW. We finally tested glucans extracted from Pleurotuseryngii grown on a substrate containing increasing concentrations of olive mill solid waste (OMSW) contain greater glucan concentrations as a function of OMSW content. Treatment of rat Intestinal epithelial cells (IEC-6) transiently transfected with Nf-κB fused to luciferase demonstrated that glucans extracted from P. eryngii stalks grown on 80% OMSWdownregulatedTNF-α activation. Glucans from mushrooms grown on 80% OMSW exerted the most significant reducing activity of nitric oxide production in lipopolysaccharide (LPS) treated J774A.1 murine macrophages. The isolated glucans were tested in vivo using the Dextran Sodium Sulfate (DSS) induced colitis in C57Bl/6 mice and found to reduce the histology damaging score resulting from DSS treatment. Expression of various intestinal cytokines were efficiently downregulated by treatment with the fungal extracted glucans. We conclude that the stress-induced growing conditions exerted by OMSW induces production of more effective anti-inflammatory glucans in P. eryngii stalks.
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Annunziato, Dominick. HPLC Sample Prep and Extraction SOP v1.3 for Fungi. MagicMyco, 2023. http://dx.doi.org/10.61073/sopv1.3.08.11.2023.
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medicine, industry, and biotechnology. Fungi produce a wide range of bioactive compounds, such as alkaloids, antibiotics, antifungals, immunomodulators, anticancer agents, enzymes, and vitamins. However, these compounds are often locked inside the fungal cell wall, which is composed of chitin, a tough substance that is dif�icult to digest by humans1. Therefore, it is essential to have a good extraction technique that can break down the chitin and release the valuable compounds from the fungi, this is especially essential in the laboratory for accurate lab assays and potency determination during routine HPLC chromatography analysis. During licensure and/ or certi�ication any given lab will be required to take a pro�iciency test which gauges the lab’s pro�iciency at measuring a given matrices for accurate evaluation. They evaluate our abilities to run the gear and accurately measure the potency of what was extracted; however, at the time of this writing none existed for extraction of the fungal material itself, so this remains a variable between testing labs. It is important that we openly share our extraction techniques for evaluating fungi materials speci�ically for the clean extraction of active alkaloids for which potency can be measure via chromatography and/or spectrometry devices. In this way hopefully creating less variables between testing lab and more concise results. In this paper, we present a novel sample prep and extraction technique for fungi that uses speci�ic solvent composition in conjunction with M.A.E (microwave assisted extraction) in 75% methanol , 25% water which helps break the cell wall to release the compounds. Also used is an ultrasonication unit and vortex mixer. Our technique quickly releases all the available alkaloids for accurate chromatography measurements in just two hours, forty-�ive minutes with minimal handling. We demonstrate the effectiveness and ef�iciency of our technique by applying it to magic mushroom fruit bodies for the extraction of tryptamines namely psilocybin and its active derivative psilocin; however, this technique can be used for other species of fungi and compounds like Cordyceps/ cordycepin or Lions’ mane/ erinacines, etc.. We also compare our technique with other popular methods in terms of extraction techniques, digestion times and solvent compositions. Our results show that our technique is superior to the others in terms of time and effectiveness while pulling all the active compounds and not degrading them. Our extraction technique for fungi chromatography analysis offers a new and improved way to access the natural products of fungi and explore their potential for various biotechnological applications. We hope that our technique will inspire further research and innovation in the field of fungal extraction and natural product.