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1

Iermolenko, T. I., N. G. Ruda, and O. I. Pautina. "COMPARATIVE STUDY OF ANTI-INFLAMMATORY, ANALGESIC AND ANTI-ULCER EFFECT OF CANCARIUS MUSHROOM EXTRACT (CANTHARELLUS CIBARIUS) AND SHIITAKE MUSHROOM EXTRACT (LENTINUS EDODES) IN THE EXPERIMENT." Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії 21, no. 2 (2021): 135–41. http://dx.doi.org/10.31718/2077-1096.21.2.135.

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Mushrooms are appreciated not only for their nutritional value and bioavailability, but also for their medicinal properties. There are a growing number of the studies focused on investigating medical preparation base on natural compounds, including those isolated from a number of fungi species of fungi species. The aim of this study is to compare anti-inflammatory, analgesic and antiulcer effects of extracts of shiitake mushrooms and chanterelle mushrooms in an experiment on rats. Materials and methods. The experiment involved 30 white female rats weighing 170-210 g., divided into 5 groups: group 1 included intact animals; group 2 included animals with modelled pathology; group 3 included animals, which received chanterelle extract; group 4 involved animals, which received shiitake mushroom extract, and group-5 rats received the reference drug. Observation of changes over the inflammatory process in the model of carrageenan-induced inflammation in the paw of the rats revealed an anti-exudative effect of varying intensity that made up 36.84% for the of chanterelle mushroom extract and 15.79% for shiitake mushroom extract. The dynamics of trypsin-induced inflammatory process showed moderate anti-inflammatory activity, 32.02%, during the courses of the therapy with chanterelle mushroom extract. According to the indicator of analgesic effects produced by the studied extracts, the highest indicator was reached by the chanterelle mushroom extract (33.53%) that exceeded the analgesic effect of the shiitake mushroom extract (17.65%). Therapeutic and prophylactic administration of the chanterelle mushroom extract in a dose of 10 mg / kg in the model of aspirin-induced gastric ulcer in rats showed a pronounced anti-ulcer effect which made up 66.7% compared with that by shiitake mushroom extract (33.4%) and the reference drug “Altan” (100%). Conclusion. The obtained data have demonstrated the promise of using the extracts of chanterelle and shiitake mushrooms for the therapeutic and prophylactic purposes.
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Chanate, Wanna, Boonman Narumon, Phakpaknam Sirirat, Chutrtong Jaruwan, and Wongwiwat Piyada. "Comparative Evaluation of Chemical Compositions and Antioxidant Activity in Fruiting Bodies and Mycelia of Volvariella volvacea and Pleurotus sajor-caju Extracts." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 15, no. 03 (2024): 1499–509. http://dx.doi.org/10.25258/ijpqa.15.3.63.

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Mushrooms, recognized for their rich medicinal and nutritional content, have garnered significant interest due to their potent antioxidant properties. This study investigates the fruiting bodies and mycelia of straw mushrooms (Volvariella volvacea) and grey oyster mushrooms (Pleurotus sajor-caju). The mushrooms were extracted using distilled water at ratios of 1:25, 1:30, and 1:35 g/mL by soaking in a temperature-regulated shaking bath set at 50°C. The aqueous extracts were analyzed for their bioactive components, including polysaccharides, proteins, GABA, total phenolic content (TPC), and total flavonoid content (TFC), along with their antioxidant capacities using DPPH and ABTS assays. The results indicated that the straw mushroom exhibited higher levels of polysaccharides, GABA, TPC, and TFC compared to the grey oyster mushroom, which contained higher protein levels. Specifically, the fruiting bodies extract of the straw mushroom demonstrated the highest levels of polysaccharides (416.94 mg/g extract), GABA (12.86 mg/g extract), TPC (33.27 mg GAE/g extract), and TFC (9.39 mg CE/g extract). Furthermore, the fruiting bodies extract showed a greater capacity to scavenge free radicals than the mycelia extract of both straw mushroom and grey oyster mushroom, with DPPH assay values of 0.65 and 0.47 mg TE/g extract and ABTS assay values of 2.26 and 1.74 mg TE/g extract, respectively. These results suggest that the fruiting bodies possess higher free radical scavenging potential as an outcome of their high levels of bioactive compounds. Consequently, these extracts indicate promise as natural antioxidative agents and could be effectively utilized as ingredients in the culinary industry.
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3

Ng, S. P., R. Ramanathan, and X. J. Chong. "The effect of cooking duration on radical scavenging properties of Hypsizygus tessellatus and Pleurotus ostreatus." Supplementary 1 6, S1 (2022): 75–79. http://dx.doi.org/10.26656/fr.2017.6(s1).013.

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Mushrooms are a popular ingredient in the human diet due to their flavour, nutritional values and functional properties, in particular, their antioxidant potential. Most mushrooms are consumed after cooking and the impact of cooking methods and durations on the antioxidant properties of various edible mushrooms have been reported. However, the reports on the effect of cooking on the antioxidant properties of Hypsizygus tessellatus (shimeji), a widely consumed mushroom in East Asia, are limited. Therefore, this study aimed to investigate the effect of cooking duration on the radical scavenging properties of H. tessellatus. Pleurotus ostreatus was included for comparison purposes. Mushroom samples were prepared raw, boiled in distilled water for 1 min, 3 mins and 5 mins, then blended and centrifuged to obtain mushroom extracts. The mushroom extracts were evaluated for their radical scavenging properties using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Results showed that 1-min cooked H. tessellatus extract exhibited the highest radical scavenging activity (76.6±2.14% DPPH scavenging activity) while extract boiled for 5 mins had the lowest radical scavenging activity (62.9±6.08% DPPH scavenging activity). Thus, cooking time has a significant effect on the radical scavenging activity of H. tessellatus extract. In conclusion, the effect of cooking on H. tessellatus and P. ostreatus can be both beneficial and disadvantageous depending on the duration.
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Sunthudlakhar, Punjaporn, Pongtip Sithisarn, Bhusita Wannissorn, Siripen Jarikasem, and Piyanuch Rojsanga. "Phytochemical Profiles, Antioxidant and Antibacterial Activities of 11 Phellinus Mushrooms Collected in Thailand." Natural Products Journal 9, no. 2 (2019): 144–56. http://dx.doi.org/10.2174/2210315508666180621160917.

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Background:Phellinus mushrooms are locally available in Thailand. They have been traditionally used for medicinal purposes including tonics, for cancer treatment and for immune system stimulation. </P><P> Objective: To evaluate the in vitro antioxidant, antibacterial activities and the phytochemical information of the extracts from 11 selected Phellinus mushrooms collected in Thailand.Methods:Eleven Phellinus mushrooms were collected from the Northeastern part of Thailand. Their ethanol and water extracts were tested for in vitro antioxidant activities using a DPPH scavenging assay, FRAP assay and TBARS method while the antibacterial effects were determined by agar disk diffusion and broth microdilution methods. The phytochemical profiles of the Phellinus mushroom extracts were evaluated using TLC and HPLC techniques. The total phenolic and total flavonoid contents of the extracts were evaluated by Folin-Ciocalteu and aluminium chloride methods, respectively. While total carbohydrate and total triterpene contents were evaluated by phenolsulfuric acid and vanillin perchloric acid methods, respectively.Results:Most ethanol extracts from Phellinus mushrooms exhibited moderate antioxidant effects especially extracts from P. everhartii, P. hippophaëicola and P. pini var. microporus while P. igniarius var. cinereus ethanol extract exhibited inhibitory effects to Helicobacter pylori both normal and resistant strains, this extract also showed the strongest inhibitory effect against Staphylococcus aureus. Most Phellinus mushroom extracts exhibited similar TLC and HPLC fingerprints with the chromatographic bands corresponding to phenolics, flavonoids and terpenoids. Protocatechuic acid was identified in most Phellinus mushroom extracts.Conclusion:Phellinus mushrooms especially, P. everhartii could be developed as sources of antioxidants while P. igniarius var. cinereus indicated the potential to inhibit H. pylori. Protocatechuic acid could be used as a marker for quality control of Phellinus mushroom raw materials and extracts.
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HAMDAN, Noor T. "FIRST REPORT OF THE ANTICANCER EFFICIENCY OF AGARICUS BRASILIENSIS MUSHROOM ON HUMAN EMBRYONIC LIVER WRL68 AND HUMAN PANCREATIC ASPC-1 CANCER CELLS THERAPY." MINAR International Journal of Applied Sciences and Technology 03, no. 02 (2021): 28–35. http://dx.doi.org/10.47832/2717-8234.2-3.4.

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Mushrooms are food traditionally consumed in Asia, Europe and America. They are being studied for medicinal benefits. Extensive studies have shown that Agaricus brasiliensis mushroom used as a medical productto combat cancers. Our data reveal that the determined inhibitory concentration fifty(IC50) values were observed maximum dose responses (IC50) of WRL68 andAsPC-1 cancer cells reported of 172.6 µg/ml and 158.2 µg/ml respectively at 2.23 and 2.1 µg/ml ethanolic mushroom concentrations. The highly cytotoxic activity of the extract on growth inhibition AsPC-1 and WRL68were generally observed 97.9% and 95% at extract concentrations of 25 µg/ml and 50µg/ml respectively. Finally, Phytochemical profile of Agaricus brasiliensis mushroom extract found to beflavonoids, glycosides, saponins, phenols,alkaloids, tannins.The extracts of Agaricus brasiliensiswas tested throughGas Chromatography-Mass(GC-MS). There were five different compounds analyzed from the extracts of Agaricus brasiliensis. The compounds in the ethanolic extract of A. brasiliensis mushroom were comprised mostly of Acetic acid ethyl ester(38.39%),followed by(3-Methyl-oxiran-2-yl)-methanol (34.71%), Chlorbromuron (22.86%), n-Hexadecanoic acid (3.85%) and Heptane, 1-(1-butenyloxy) (0.19%). However, no studies were done using Agaricus brasiliensismushroom extract against WRL68 and AsPC-1 human cell lines. Therefore, the anticancer efficiency of Agaricus brasiliensis mushroom on human embryonic liver WRL68 andhuman pancreatic AsPC-1 cancer cellstherapy is being reported for the first time in the current study.
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6

R, Madhanraj, Ravikumar K, Maya MR, et al. "Evaluation of anti-microbial and anti-haemolytic activity of edible basidiomycetes mushroom fungi." Journal of Drug Delivery and Therapeutics 9, no. 1 (2019): 132–35. http://dx.doi.org/10.22270/jddt.v9i1.2277.

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Mushrooms are used for a variety of biotechnological applications, particularly for the production of food, enzymes, dietary supplements, pharmaceutical compounds, feed supplements. Basidiomycetes mushroom fungi have been known for their nutritional values they can be considered as functional foods which can provide health benefits beyond the traditional nutrients. Mushroom represents a major and untapped source of potent new pharmaceutical products. A wide range of activities including antitumour, cardiovascular and antimicrobial are reported in mushrooms. In developing countries like India mushroom progress is a boon in the field of food, medicine, and in generating employment. The alternative systems of medicine utilize the curative properties of mushrooms. They are also known to possess promising antioxidative, cardiovascular, hypercholesterolemia, antimicrobial, hepato-protective and anticancer effects. The present study aimed to evaluate antimicrobial and antihaemolytic activities of various extracts of Basidiomycetes mushroom fungi. Different Basidiomycetes mushroom fungi extracts (Hot water, Acetone and Hexane) were tested for antimicrobial and antihaemolytic activities. Among these different extracts of Basidiomycetes mushroom fungi the hexane extract of Pleurotus pulmonarius showed potential antimicrobial and antihaemolytic activity. The results of this study suggest the possibility of using Basidiomycetes mushroom fungi as natural sources for the pharmaceutical industry and could act as safe and cost-effective with potential biological activities. These findings encourage studying these fungal strains further for their potential biological applications.
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7

Sitati, Concepta N. W., Kenneth O. Ogila, Rebecca W. Waihenya, and Lucy A. Ochola. "Phytochemical Profile and Antimicrobial Activities of Edible Mushroom Termitomyces striatus." Evidence-Based Complementary and Alternative Medicine 2021 (October 19, 2021): 1–10. http://dx.doi.org/10.1155/2021/3025848.

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The mushroom Termitomyces striatus is an edible mushroom that grows wildly and belongs to the family Lyophyllaceae. Studies in the last few decades have demonstrated that mushrooms and their active components have beneficial effects on a variety of biological systems. Some mushrooms do exhibit antibacterial properties. Qualitative phytochemical profile was done on the mushroom Termitomyces striatus to establish the presence of compounds responsible for important biological activities. This study also investigated the effect of Termitomyces striatus extracts on certain bacterial strains that included Escherichia coli and Pseudomonas aeruginosa representing the Gram-negative bacteria and Bacillus subtilis and Staphylococcus aureus representing Gram-positive bacteria. The fungi were represented by Candida albicans and Saccharomyces cerevisiae. The mushroom was collected in western Kenya, air-dried, and crushed into powder, followed by extraction using water, methanol, and dichloromethane (DCM) solvents. Antibacterial and antifungal activities were evaluated using the disc-diffusion method. Qualitative phytochemical screening of the aqueous extract revealed the presence of alkaloids, flavonoids, steroids, sterols, saponins, phenols, carbohydrates, and proteins. The three extracts exhibited antibacterial against tested bacterial strains. The DCM extract revealed higher effects among the bacterial strains tested. The three extracts showed antifungal effects against C. albicans. However, both methanol and aqueous extracts did not inhibit growth of S. cerevisiae. In conclusion, T. striatus extracts are a promising source of novel antimicrobial and antifungal agents.
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Nurkanti, Mia, Mimi Halimah, Yosi Silfister, and Handi Suganda. "Potensi Ekstrak Lengkuas (Alpinia Purpurata K. Schum) Sebagai Fungisida Terhadap Jamur Alternaria Sp. Pada Tanaman Jeruk." Biotropic : The Journal of Tropical Biology 4, no. 2 (2020): 111–18. http://dx.doi.org/10.29080/biotropic.2020.4.2.111-118.

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Alternaria sp. is one the causess of diseases that attack citrus crops. Pest and diseases control efforts by farmers more often use pesticide synthesis. This study aims to determine the inhibition of mushroom growth and other benefits of galangal extract as a natural fungicide and determine the optimum concentration of galangan extract inhibiting the growth mushroom of Alternaria sp.. The method used was experimental, testing conducted by growing mycelium mushroom Alternaria sp. on a PDA that has been mixed with extract of galangal in according to the respective concentrations and analyze growth response by measuring the diameter. The best results indicated by the treatments 60% that control the growth of mushrooms with deaths amounted to 79%. This shown that galangal extract is able to control the growth of Alternaria sp. at concentrations 60% compared to a concentration of 20%, 30%, 40% and 50% because the active compounds at concentrations that are 60% higher, so galangal extract diffuse into the cell the growing of mushrooms and may cause disruption the growth mushrooms Alternaria sp.. The higher concentration of extract given, the ability to controlling the growth mushroom of Alternaria sp. the better. The utilization of galangal material is done as an alternative fungicide in the control of plant diseases that are eco friendly.
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9

Bakır, Temel kan, Mansor Boufars, Mertcan Karadeniz, and Sabri Ünal. "AMINO ACID COMPOSITION AND ANTIOXIDANT PROPERTIES OF FIVE EDIBLE MUSHROOM SPECIES FROM KASTAMONU, TURKEY." African Journal of Traditional, Complementary and Alternative medicines 15, no. 2 (2018): 80. http://dx.doi.org/10.21010/ajtcamv15i2.10.

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Background: Hydnum repandum, Cantharellus cibarius, Ramaria fennica, Boletus edulis, and Craterellus cornucopioides are all wild edible mushroom species from the Kastamonu province. The aim of this study was to investigate antioxidant properties and amino acid contents of these mushrooms.
 Materials and Methods: The mushrooms were analyzed for their free amino acid compositions using a high performance Amino Acid analyzer (Biochrom 30). Also, antioxidant activity and total phenolic concentrations of five different mushroom species were studied by using spectrophotometric methods.
 Results: The mushrooms contained 17 amino acids (g/100 g): Glutamic acid (2.56–1.11), Alanine (1.49–0.54), Arginine (1.62–6.77), Aspartic acid (1.45–0.81), Leucine (1.08–0.64), Methionine (1.05–0.06), Valine (1.05–0.66), Lysine (1.01–0.57), Serine (0.68–0.38), Cysteine (0.88–0.11), Isoleucine (0.61–0.39), Glycine (0.82–0.35), Threonine (0.82–0.44), Phenylalanine (0.66–0.42), Proline (0.60–0.47), Tyrosine (0.58–0.38), and Histidine (0.48–0.22). The total free amino acid (TAA) contents ranged from 169.2 mg/g in Boletus edulis to 89.1 mg/g in Hydnum repandum. These five different mushroom species contain eight essential amino acid species except tryptophan. The antioxidant activity of mushroom extracts was expressed as the percentage of DPPH radical inhibition and IC50 values (mg/mL). The percentage of inhibition ranged from 2.38% to 88.05% and IC50 values ranged from 0.03 to 13.98 mg/mL. The total phenolic content ranged from 0.66 to 7.74 mg/g of dry mushroom extract, expressed as gallic acid equivalents.
 Conclusion: Methanolic extract of Boletus edulis showed the highest phenolic content and strong antioxidant activity. As a result, the significant linear correlation between the values for the total phenolic content and antioxidant activity of mushroom extracts was corroborated in all of the investigated mushroom species, with the exception of Craterellus cornucopioides.
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Iguchi, Kazuaki, Koji Nagashima, Jun Mochizuki, Hiroyuki Yamamoto, Keiko Unno, and Noriyuki Miyoshi. "Enokitake Mushroom and Its Active Component, Adenosine, Which Restores Testosterone Production in Impaired and Fatigued Mouse Models." Nutrients 15, no. 9 (2023): 2142. http://dx.doi.org/10.3390/nu15092142.

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Several studies have reported the effects of the consumption of various mushroom species on the testes in animal experimental models. Mushrooms, including enokitake mushrooms (Flammulina velutipes), and vegetables contain adenosine may affect testosterone production. Here, we aimed to elucidate the effects of enokitake and its active component, adenosine, on testosterone production in primary cultures of testicular cells in vivo using mice models and in vitro. The administration of enokitake ethanolic extract increased testosterone production in the cisplatin-impaired mouse model. The direct effect of mushroom extracts on testicular cells was examined and liquid chromatography–mass spectrometry analysis confirmed that the mushroom- and vegetable-induced increase in testosterone production mainly involved adenosine. Additionally, the administration of enokitake extract or adenosine to wet floor fatigue model mice promoted testicular testosterone production and enhanced Leydig cell function through insulin-like peptide three level upregulation. Structurally related compounds, including cordycepin, showed lower bioactivity than adenosine. This study showed that the ingestion of adenosine-containing mushrooms and vegetables may effectively increase testicular testosterone production. We conclude that mushrooms with a relatively high adenosine content, such as enokitake, may be useful against aging and fatigue.
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Medvedkova, I. I., and N. A. Popova. "Main risks during storage of mushroom food products." Tovaroved prodovolstvennykh tovarov (Commodity specialist of food products), no. 3 (February 27, 2025): 177–80. https://doi.org/10.33920/igt-01-2503-09.

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The main risks arising when storing mushroom products are associated with insuffi cient development of storage infrastructure. Guided by the HACCP principles, the main methods of processing and storing mushrooms were identifi ed: freezing, drying mushrooms, mushroom powder, mushroom extract, salting, pickling, and canning in hermetically sealed containers. Methods of storage that are not included in this classification will be considered by us in order to determine and justify the most optimal method of storing mushroom products.
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12

Al-Dbass, Abeer M., Sooad Al Daihan, Aisha A. Al-Nasser, et al. "Biogenic Silver Nanoparticles from Two Varieties of Agaricus bisporus and Their Antibacterial Activity." Molecules 27, no. 21 (2022): 7656. http://dx.doi.org/10.3390/molecules27217656.

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Agaricus bisporus, the most widely cultivated mushroom, is safe to eat and enriched with protein and secondary metabolites. We prepared silver nanoparticles (AgNPs) from two varieties of A. bisporus and tested their antibacterial activity The synthesized AgNPs were initially confirmed by UV-Vis spectroscopy peaks at 420 and 430 nm for white and brown mushrooms AgNPs, respectively. AgNPs were further characterized by zeta sizer, transmission electronic microscopy (TEM), Fourier transform infrared (FTIR), and energy-dispersive X-ray spectroscopy (EDX) prior to antibacterial activity by the well diffusion method against six bacterial strains which include Staphylococcus aureus, Staphylococcus epidermis, Bacillus subtilis, Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa. TEM results revealed a spherical shape with an average diameter of about 11 nm in the white mushroom extract and 5 nm in the brown mushroom extract. The presence of elemental silver in the prepared AgNPs was confirmed by EDS. The IR spectrum of the extract confirmed the presence of phenols, flavonoids, carboxylic, or amide groups which aided in the reduction and capping of synthesized AgNPs. The AgNPs from both extracts showed almost the same results; however, nanoparticles prepared from brown mushrooms were smaller in size with strong antibacterial activity.
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13

Dewi, Ni Kadek Emi Sintha, I. Gede Putu Wirawan, and Made Sritamin. "MORPHOLOGICAL IDENTIFICATION OF BACTERIA ISOLATED FROM MUSHROOM BAGLOG AND ITS EFFECT ON SHIITAKE (Lentinula edodes (Berk.) Pegler) AND REISHI (Ganoderma lucidum (Curtis) P. Karst) MYCELIAL GROWTH." International Journal of Biosciences and Biotechnology 9, no. 1 (2021): 31. http://dx.doi.org/10.24843/ijbb.2021.v09.i01.p04.

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Reishi (Ganoderma lucidum (Curtis) P. Karst) and shiitake (Lentinula edodes (Berk.) Pegler) mushrooms have high economic value and contain bioactive metabolites that provide heatlh benefits. Shiitake mushrooms have a delightfully unique taste as an edible mushroom. Mushroom production can be increased by utilizing microbes known as MGPB (Mushroom Growth Promoting Bacteria). This study aimed to determine the presence of bacteria associated with shiitake and reishi mushrooms in the baglog and determine how these bacteria influence the growth of shiitake and reishi mycelium. Bacteria was isolated from the mushroom baglog then were purified and centrifuged to obtain the cell-free extract. Furthermore, the bacterial cell-free extracts were poured on PDA media, then shiitake and reishi mycelium were inoculated to test the effect of bacteria isolates on fungal growth. The results showed that three isolates have the potential as MGPB in reishi mushroom, i.e., isolate S1C, S1D, and S2A. Reishi mycelium grown on isolate S2A achieved the fastest maximum growth on day 6th. In shiitake mushroom, it was seen that all isolates showed a negative effect on mycelium growth. Shiitake mycelium in isolate S1C, S1D, S2A, and S2B were seen to form a clear zone on PDA medium.
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Abd Razak, Dang Lelamurni, Anisah Jamaluddin, Nur Yuhasliza Abd Rashid, Nor Ajila Sani, and Musaalbakri Abdul Manan. "Assessment of Cosmeceutical Potentials of Selected Mushroom Fruitbody Extracts Through Evaluation of Antioxidant, Anti-Hyaluronidase and Anti-Tyrosinase Activity." J—Multidisciplinary Scientific Journal 3, no. 3 (2020): 329–42. http://dx.doi.org/10.3390/j3030026.

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Cosmeceutical formulations containing naturally derived active ingredients are currently preferred by consumers worldwide. Mushrooms are one of the potential sources for cosmeceutical ingredients but relevant research is still lacking. In this study, hot- and cold-water extractions were performed on four locally-cultivated mushrooms—Pleurotus ostreatus, Ganoderma lucidum, Auricularia polytricha and Schizophyllum commune—with the aim to assess the cosmeceutical potential of these mushroom fruitbody extracts. Total phenolics, polysaccharide and glucan content were determined. Antioxidant property of the mushroom extracts was assessed by determining the DPPH radical scavenging, ferric-reducing (FRAP) and superoxide anion (SOA) scavenging activity. Anti-hyaluronidase activity was used as an indicator for the anti-aging and anti-inflammatory property, while anti-tyrosinase activity was evaluated to assess the anti-pigmentation or whitening property of these extracts. Our results showed that total polysaccharide content of P. ostreatus extracts was the highest (235.8–253.6 mg GE/g extract), while extracts from G. lucidum contained the lowest glucan (10.12–10.67%). Cold-water extract from S. commune exhibited substantial tyrosinase inhibition activity (98.15%) and SOA scavenging activity (94.82%). The greatest hyaluronidase activity was exhibited by G. lucidum hot-water extract, with the value of 72.78%. The findings from the correlation analyses suggest that the cosmeceutical properties of these mushrooms can be attributed mainly to the combination of different types of compound such as polysaccharides and phenolics. Overall, cold-water extract of S. commune and hot-water extract of G. lucidum showed the best results and may be further investigated.
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JASIŃSKA, Agnieszka, Luiza DAWIDOWICZ, Marek SIWULSKI, and Patryk KILINOWSKI. "Growth of Mycelium of Different Edible and Medicinal Mushrooms on Medium Supplemented with Digestate from AD Biogas Plant." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 45, no. 2 (2017): 498–506. http://dx.doi.org/10.15835/nbha45210954.

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Digestate is remaining material after anaerobic digestion (AD) of a biodegradable feedstock. The AD process produces two main products: digestate and biogas; digestate is considered as a waste; however, it found application in agriculture i.e. as a soil conditioner or cultivation substrate component. Digestate is rich in nitrogen and phosphorus and can be used as a substrate component for mycelial growth of mushrooms. Aim of the study was examination of agar media supplementation with digestate from AD of foodwaste material on mycelial growth of cultivated and medicinal mushrooms. Mycelial growth of four mushroom species was investigated: Coprinus comatus, Ganoderma lucidum; Agaricus subrufescens and Laetiporus sulphureus. First experiment investigated four mushroom species and 3 digestate-only based agar media with growing amount of digestate extract. Later fastest growing mushrooms from exp. 1, C. comatus and G. lucidum, was chosen, mycelial growth was performed on 2 digestate-only based agar media and 2 what/digestate extract based agar media. Wheat-based and manure-based agar media was used as control medium. Study confirmed positive effect of digestate from AD as an addition or main component of agar media culture for mushroom mycelium growth. The amount of digestate extract affected mycelial growth of investigated species. The best agar media was digestate-based only with 25 g of extract and wheat/digestate based (150/50 g extract). The fastest and the best growth were obtained for C. comatus. The residue from AD biogas production could have found additional application as media product for mushroom mycelia production and further as a component for mushroom cultivation substrate.
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PEREZ, JEBBEN, and CANDELARIO CALIBO. "EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF VOLVARIELLA (Volvariella volvacea), WOOD EAR (Auricularia auricula-judae), AND SHIITAKE (Lentinula edodes) MUSHROOMS." Science and Humanities Journal 11, no. 1 (2017): 91–101. http://dx.doi.org/10.47773/shj.1998.110.5.

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Edible mushrooms, whether fresh or processed, are widely consumed worldwide. In order to determine their nutritional and/or functional values, this study was conducted to evaluate the free radical scavenging activity of Volvariella (Volvariella volvacea), wood ear (Auricularia auricula-judae), and shiitake (Lentinula edodes) mushrooms. Trolox equivalent per 100g sample (TE/100g) was determined to describe the activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results showed that wood ear exhibited the highest free radical scavenging activity (387.11±30.26 TE/100g) among the three species. Shiitake mushroom exhibited 368.13 ±27.03 TE/100g activity. Volvariella contained the lowest activity with 318.72 ±36.87 TE/100g. Based on the solvent system, ethanolic extracts showed the highest free radical scavenging activity (386.45±23.30 TE/100g) while hexane extract showed the least free radical scavenging activity (326.34 ±27.75 TE/100g). Water extract exhibited 361.17 ±49.11TE/100g activity. The results of this study showed that mushroom extracts could serve as natural antioxidants due to their significant antioxidative property
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Angelini, Paola, Giancarlo Angeles Flores, Gaia Cusumano, et al. "Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of Hypsizygus marmoreus." Microorganisms 11, no. 10 (2023): 2552. http://dx.doi.org/10.3390/microorganisms11102552.

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The beech mushroom (Hypsizygus marmoreus) is a highly nutritious, edible medicinal mushroom native to East Asia. The present research investigated the impact of different substrates on the metabolite compositions of H. marmoreus mycelia cultivated in vitro. The substrates tested included malt extract agar, malt extract agar enriched with barley malt, and malt extract agar enriched with grape pomace. The study also assessed antimicrobial and antiradical activities of the extracts against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), gram-negative bacteria (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa), yeasts (Candida albicans, C. tropicalis, and C. parapsilosis), and dermatophytes (Trichophyton mentagrophytes, T. tonsurans, T. rubrum, Arthroderma quadrifidum, A. gypseum, A. curreyi, and A. insingulare). The results revealed that the H. marmoreus mycelia extracts demonstrated antibacterial and antifungal activities against the tested microorganisms. Extracts obtained from the cultivation in substrates enriched with either barley malt or grape pomace exhibited the highest antibacterial activity among all the tested bacterial strains except for P. aeruginosa. The same extracts showed the highest inhibitory effect against C. albicans and C. parapsilosis. Noteworthy, the extract from the mushroom cultivated in the substrate enriched with grape pomace also exhibited remarkable efficacy against T. mentagrophytes and T. tonsurans. Terpenoid and carbapenem compounds could be related to the antimicrobial properties of the extracts from mushrooms cultivated in substrates enriched with grape pomace. In comparison, the higher antiradical properties could be related to the content of indole compounds. In conclusion, growth substrate selection affects the nutritional and medicinal properties of H. marmoreus, making it a valuable contribution to the understanding of the cultivation of this mushroom.
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Iqbal, Touseef, Muhammad Sohaib, Sanaullah Iqbal, and Habib Rehman. "Exploring Therapeutic Potential of Pleurotus ostreatus and Agaricus bisporus Mushrooms against Hyperlipidemia and Oxidative Stress Using Animal Model." Foods 13, no. 5 (2024): 709. http://dx.doi.org/10.3390/foods13050709.

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The mushrooms oyster (Pleurotus ostreatus) and white button (Agaricus bisporus) contain bioactive compounds that have potential beneficial effects on hypercholesterolemia and cardiovascular diseases. In this study, hypolipidemic and antioxidative potential of these mushrooms’ extract were explored using hypercholesterolemic (HC) rats as animal model. For the study, 56 adult rats were divided into seven groups, i.e., G1 (negative control), G2 (positive control group), G3 (HC rats with statin drug orally), G4 and G5 (HC rats @ 100 and 200 mg/kg body weight (BW) dose of oyster mushroom extracts), and G6 and G7 (HC rats @ 100 and 200 mg/kg BW dose of white button mushroom extracts). The hypercholesterolemia was induced experimentally in fasted rats through a high-fat diet along with injection of triton WR-1339. After 48 h, the treatment groups were given extract for 28 days along with standard diet. At the trial termination, we analyzed the blood sugar levels, antioxidant parameters, lipid profile, and renal function, as well as conducting liver function tests of the rats. The results indicated that positive control group rats exhibited increased levels of total cholesterol (TC), triglycerides (TG), low-density level (LDL), and very-low-density level (vLDL) by 19%, 37%, 52%, and 32%, respectively, and 53% decrease in HDL, whereas treatment groups that received 200 mg oyster and white button mushroom extracts reported 15%, 34%, 22% reduction in TC, TG, vLDL, respectively, and 22% improvement in HDL level. The enzyme profiles of different groups showed non-significant differences, although both mushroom extracts provision reduced glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels. Overall, the results indicated that mushroom extracts were helpful in maintaining oxidative stress and have the potential to improve dyslipidemia in the tested rat animal model.
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Apra, Meilani, Aniek Prasetyaningsih, and Kukuh Madyaningrana. "POTENSI BIOAKARISIDA EKSTRAK DAUN MIMBA (Azadirachta Indica) DAN BUNGA CENGKEH (Syzygium Aromaticum) TERHADAP TUNGAU PENYEBAB PENYAKIT KREPES PADA JAMUR KUPING." EduMatSains : Jurnal Pendidikan, Matematika dan Sains 5, no. 2 (2021): 225–38. http://dx.doi.org/10.33541/edumatsains.v5i2.2241.

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Wood ear mushrooms is commonly known as one of the cultivated edible mushrooms in Indonesia. The demand for this commodity is still steadily high and leads mushroom farmers to optimize its cultivation. For mushroom farmers in Yogyakarta, the prevalence of krepes disease which is caused by a group of species belongs to Acarina order is still high and leads to total loss of harvest. In order to diminish these Acarina species, the use of chemical acaricides is common because of their effectiveness in eradicating the pests despite their toxic effect to the environment. A strategy to provide environmental friendly acaricides which are extracted from plants is therefore important. This research aimed to study the potency of neem leaves (Azadirachta indica) and clove flowers (Eugenia caryophyllata) extracts as bioacarisides for Acarina species as the causal agent of krepes disease. Neem leaves and cloves flowers were extracted using maceration and soxhlet extraction methods, using 96% methanol as their solvents. Obtained crude extracts would then be subjected to phytochemical analysis using qualitative biochemical tests and GC-MS. The effectivity of extracts as bioacaricides were tested both in in vitro and field scale. In vitro tests showed that neem leaves extract and clove flower extract had LC50 value ​​of 0.22% and 0.48% respectively. Field scale test on mushroom baglogs showed that the efficacy of the neem leaves and clove flower to eradicate acarina were above 50%. Taken together, neem leaves and clove flower extracts are potential to be used as bioacaricides in mushroom farming.
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Mohd Jamil, Nor Azreen, Norasfaliza Rahmad, Noraswati Mohd Nor Rashid, Mohd Hafis Yuswan Mohd Yusoff, Nur Syahidah Shaharuddin та Norihan Mohd Saleh. "LCMS-QTOF Determination of Lentinan-Like β-D-Glucan Content Isolated by Hot Water and Alkaline Solution from Tiger’s Milk Mushroom, Termite Mushroom, and Selected Local Market Mushrooms". Journal of Mycology 2013 (18 грудня 2013): 1–8. http://dx.doi.org/10.1155/2013/718963.

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Lentinan, 1152 Dalton β-D-glucan found in Shiitake Mushroom (Lentinus edodes), has been claimed to have anticancer and immunomodulatory activity. Several extraction methods have been used by researchers to isolate Lentinan including hot water and alkaline solution (1.25 M NaOH). In this study, hot water and alkaline solution (1.25 M NaOH) were used to extract the Lentinan-like β-D-glucan (1151 Dalton) from Tiger’s Milk Mushroom, Termite Mushroom, and selected local market mushrooms. The isolated Lentinan-like β-D-glucan from both hot water and alkaline solution was analyzed by LCMS-QTOF. Commercial Lentinan standard from Lentinus edodes was used as a reference. The results showed significant differences on chromatogram patterns of Lentinan-like β-D-glucan between both extracts. The peak of Lentinan-like β-D-glucan was only found in isolated polysaccharide glucan of hot water extracts. The isolated polysaccharide glucans from Tiger’s Milk Mushroom and Termite Mushroom were found to have 0.74±0.12 μg/mg and 0.53±0.07 μg/mg Lentinan-like β-D-glucan. Button Mushroom, Shiitake Mushroom, and Oyster Mushroom showed the presence of Lentinan-like β-D-glucan at 16.16±4.15 μg/mg, 0.22±0.04, and 0.10±0.01 μg/mg, respectively.
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Abdelkader, Menna-Allah E., Hatungimana Mediatrice, Dongmei Lin, Zhanxi Lin, and Sarah A. Aggag. "Mitigating Oxidative Stress and Promoting Cellular Longevity with Mushroom Extracts." Foods 13, no. 24 (2024): 4028. https://doi.org/10.3390/foods13244028.

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Oxidative stress can disrupt the body’s ability to fight harmful free radicals, leading to premature aging and various health complications. This study investigated the antioxidant and anti-aging properties of four medicinal and edible mushrooms: Ganoderma lucidum, Hericium erinaceus, Pleurotus ostreatus, and Agaricus bisporus. The antioxidant activity of mushroom extracts was evaluated using (DPPH-ABTS-Reducing power). The anti-aging effects were assessed using Human Skin Fibroblasts (HSF) cells subjected to D-galactose-induced aging (30 g/L/72 h) and treated with mushroom extracts (0.03–0.25 mg/mL/72 h). The results demonstrated that all mushrooms have significant antioxidant and anti-aging properties, with low concentrations of extracts (0.03 mg/mL) effectively promoting cell proliferation at an 87% rate in the Agaricus bisporus extract, enhancing cell cycle progression by reducing the arrested cells in the G0/G1 phase to 75%, and promoting DNA synthesis in S phase by more than 16.36% in the Hericium erinaceus extract. Additionally, the extracts reduced DNA damage and Reactive Oxygen Species (ROS) levels, protecting cells from oxidative stress and potentially contributing to anti-aging effects. The mushrooms also exhibited immunomodulatory and anti-inflammatory effects by upregulating the IL-2, IL-4, and downregulating IL-6 expression, indicating their potential to promote general health. These findings suggest the potential of mushroom extracts as natural agents for reducing the negative effects of aging while promoting cellular health. Further research is required to explore the specific bioactive compounds responsible for these beneficial effects and to evaluate their efficacy in vivo.
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Febriansyah, E., I. Saskiawan, R. Riffiani, R. R. Elfirta, R. H. Setyawan, and Kasirah. "Effect of Bacillus aryabhattai extract addition on growth and productivity of Pleurotus ostreatus ((Jacq ex Fr.) P. Kumm)." Food Research 8, Supplementary 2 (2024): 46–49. http://dx.doi.org/10.26656/fr.2017.8(s2).17.

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Pleurotus ostreatus (White oyster mushroom) is one of the most widely cultivated mushrooms in the world. Nutrients provided in the media are one of the most important factors affecting the growth of mushrooms. The present study mainly focuses on the isolation of mushroom growth-promoting (MGP) bacteria and studies its effects on the growth of mushroom fruiting bodies. Bacillus aryabhattai was isolated from the growth media of Pleurotus ostreatus, identified based on 16S rDNA sequencing and phylogenetic tree construction by the neighbor-joining method using MEGA version 6.06 and its effect on fruiting body growth of the P. ostreatus was investigated. The results of this research strongly suggest that the addition of specific bacteria supernatant into the mushroom growth media has beneficial applications for mushroom production.
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Chinonso, Ukaegbu Ishmael, Jalal K. C. A, Shah Samiur Rashid, Shaheen Sarkar, Hazrulrizawati Abd Hamid, and Azmi N. S. "BETWEEN THE BIOACTIVE EXTRACTS OF EDIBLE MUSHROOMS AND PHARMACOLOGICALLY IMPORTANT NANOPARTICLES: NEED FOR THE INVESTIGATION OF A SYNERGISTIC COMBINATION - A MINI REVIEW." Asian Journal of Pharmaceutical and Clinical Research 10, no. 3 (2017): 13. http://dx.doi.org/10.22159/ajpcr.2017.v10i3.15406.

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ABSTRACTThe pharmacological potential of bioactive compounds extracted from mushrooms has been studied to a reasonable level. In the same vein, thebioactivity of nanoparticles has also been investigated and reported to be of potential pharmacological benefit. No doubt, there is a reasonableamount of claims regarding the vast activity of the mushroom extracts and nanoparticles on the tested cell lines and microorganisms. In this paper,a review of the recent application of bioactive compounds extracted from two edible mushrooms (Coprinus comatus and Lactarius deliciosus), aswell as some of the recently reported studies on some nanoparticles of pharmacological potentials, was carried out. In order to check for synergy inthe bioactivity of the mushroom extracts when co-administered with nanoparticles, an investigation on the synergistic application of the materialsthrough the encapsulation of the bioactive extracts from the mushroom onto the nanoparticle was proposed. The supposed synergy in the activity ofthe extract-nanoparticle complex could hold the key to improved activity of nutraceuticals against resistant microorganisms and tumor cells.Keywords: Coprinus comatus, Lactarius deliciosus, Nanoparticles, Synergy, Bioactive extracts.
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24

Hulyadi, Hulyadi, Muhali Muhali, and Masiah Masiah. "Identification of The Addition of Bran and Shallot Extract to the Quantity of Merang Mushroom Production." Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram 11, no. 1 (2023): 124. http://dx.doi.org/10.33394/j-ps.v11i1.6546.

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This study aims to identify the addition of bran and onion extract to the quantity of merang mushroom production. This research was carried out at the mushroom house merang Dusun Repok Tunjang Taman Indah Village, Pringgarata District, Lombok center NTB. This research is a descriptive experiment. The study was conducted with treatment and control design. The treatment medium was given fine bran and onion extract while the control added coarse bran rice milling waste. The data obtained are described in the form of graphs and photos. The researchers' findings show that the quality of merang mushroom production in media needs to be 36.03% higher when compared to control media. This is supported by the growth of mycelium, a faster fine head in the treatment medium. The application of bran and onion extract to the growing medium of merang mushrooms has a positive impact on the productivity and quality of merang mushrooms.
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Putra, Ivan Permana. "ALLELOPATHIC ACTIVITY OF SOME WILD MUSHROOM IN INDONESIA." Fungal Territory 3, no. 1 (2020): 1–3. http://dx.doi.org/10.36547/ft.2020.3.1.1-3.

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To date, allelopathic activity of mushroom has not been reported in Indonesia. The aim of this study was to examine the allelopathic activity of wild mushroom extract through bioassay germination on Vigna radiata L. seed. A total of 13 wild mushrooms namely : Amanita sp.1, Armillaria sp.1, Armillaria sp.2, Auricularia sp., Clitocybe sp., Crepidotus sp., Fomitopsis sp., Ganoderma sp., Lepiota sp.1, Microporus sp., Panaeolus sp., Polyporus sp., and Rigidoporus sp. used for the material extraction.The extraction of 13 wild mushrooms was done using pure water. Pure extract (25%, 50%, and 75%) was applied by using sprayer directly on the seed for 7 days. The parameters observed including percentage of seeds germinating, length of radicles, and length of plumules. The highest germination rates as well as length of plumules and radicles were obtained in the negative control using distilled water, whilst Imperata cylindrica extract which used as positive control showed opposite result. Mushroom extract showed variation in the parameter observation. Rigidoporus sp., Daedalea sp.1, and Microporus sp. 2 seem to have the best activity of allelopathic effects, while Auricularia sp. consistently induced the highest effect of seed development. Overall, in line with few previously reports, this results can broadening the information of utilization of allelochemical properties of wild mushroom.
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26

Sadaf, Quereshi, K. Pandey A., and S. Sandhu S. "Evaluation of antibacterial activity of different Ganoderma lucidum extracts." PJSR 3, no. 1 (2010): 9–13. https://doi.org/10.5281/zenodo.8285679.

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Bioproducts of Mushroom have multi beneficial effects for human welfare. Medicinal mushrooms are widely used as traditional medicinal ingredients for the treatment of various diseases and related health problems. Most of the medicinal extracts from mushroom are different forms of polysaccharides which strengthens the immune system with little or no side effect. Medicinal mushroom research has focused on discovery of compounds that can modulate positively or negatively the biological response of immune cells. The antimicrobial activity of various solvent extracts (40Pg/ml) of Ganoderma lucidum was tested against six species of bacteria: Escherichia coli (MTCC-443), Staphylococcus aureus (MTCC-737), Klebsiella pneumoniae (MTCC2405), Bacillus subtilis (MTCC-1789) Salmonella typhi (MTCC-531) and Pseudomonas aeruginosa (MTCC-779). Acetone extract exhibited maximum antibacterial activity (31.60±0.10), while the most susceptible bacterium observed was Klebsiella pneumoniae.
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Machado-Carvalho, Liliana, Tânia Martins, Alfredo Aires, Maria José Saavedra, and Guilhermina Marques. "Antioxidant, Antibacterial, and Cosmeceutical Potential of Four Common Edible Mushrooms." Applied Sciences 13, no. 13 (2023): 7357. http://dx.doi.org/10.3390/app13137357.

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Waste generated in mushroom cultivation represents a valuable raw material with health benefits, the use of which contributes to a reduction in food waste and to the circular economy. Hydroethanolic extracts obtained by ultrasound-assisted extraction of four edible mushroom species (Agaricus bisporus var. Portobello, Boletus edulis, Lentinula edodes, and Pleurotus ostreatus) were characterized according to their antioxidant, antibacterial, and cosmeceutical potential. The extract with the best properties was incorporated into a cosmetic cream. Concerning the phenolic contents and antioxidant capacity, the extracts of A. bisporus and B. edulis stood out from the other two due to their higher levels. The compounds p-hydroxybenzoic and gallic acids were present in all mushrooms in the ranges of 0.010 to 2.554 µg/g DW and 0.032 to 0.112 µg/g DW, respectively. The extract of B. edulis inhibited all the bacterial isolates, with minimum inhibitory concentration values ranging from 5 to 20 mg/mL for Gram-positive and 10–20 mg/mL for Gram-negative strains. In the anti-hyaluronidase assay, A. bisporus extract (10 mg/mL) was the most effective, with 74.4 ± 7.5% enzyme inhibition, and was selected for incorporation into the cosmetic cream. The cream with A. bisporus extract showed significantly higher phenolic contents and antioxidant capacity than the cream without the extract. Overall, our results support the high value of mushroom reuse as a source of natural antioxidants in the cosmetic industry.
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Fadly, Dzul, Rosa Dhayan, Brigita Ratna Harsanti, Dea Malyana Putri, and Nur Endah Saputri. "Phytochemicals, Flavonoids, and Antioxidant Activity of Palm Mushrooms (Volvariella sp)." Jurnal Ilmiah Kesehatan 3, no. 3 (2021): 159–65. http://dx.doi.org/10.36590/jika.v3i3.195.

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 Palm mushroom (Volvariella sp) is an edible mushroom that grows on the empty palm oil bunches, where this waste is very abundant along with the increasing production of CPO (Crude Palm Oil) of the palm oil industry. This study aimed to determine phytochemicals, flavonoids, also their antioxidant activity. The investigation was conducted on aqueous water extract and ethanolic extract of palm mushrooms. It was revealed that palm mushrooms positively contained secondary metabolites, including flavonoids, tannins, saponins, and phenols. The ethanolic extract confirmed the highest total flavonoid content of 25,9 mg QE/g dw with an IC50 value of 19,14 ppm, then declared a powerful antioxidant among aqueous extract and BHT. It confirmed that this food could be an alternative as an antioxidant source.
 
 
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Canpolat, Sükrü, Cihan Düşgün, Elif Ildız, Elif Yürümez Canpolat, and Cemil İşlek. "Antioxidant potential and fatty acid composition of Lactarius sanguifluus." Prospects in Pharmaceutical Sciences 22, no. 4 (2024): 1–6. http://dx.doi.org/10.56782/pps.225.

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Edible mushrooms have been valued as a source of food and traditional medicines since ancient times. Mushrooms have drawn the attention of numerous researchers in recent decades since they contain a variety of bioactive compounds with antibacterial, antiallergic, antidiabetic, antitumoral, and antioxidant activities. As a member of the Russulaceae family Lactarius sanguifluus (Paulet) Fr. (1838) (also known as bloody milk cap) is an ectomycorrhizal edible mushroom distributed in Asia, the Mediterranean shores and mid-Europe. In this study it was aimed to identify the mushroom sample both by physical traits according to the current literature and by molecular techniques. The antioxidant capability of L. sanguifluus methanol extract was evaluated by stable radical scavenging activity and metal ion reducing capability methods. The content of total phenolic compounds and the fatty acid composition of the mushroom sample were also investigated. The mushroom sample was identified both by comparing the physical traits with the current literature and by comparing the sequence data of the ITS region of rDNA with GenBank. The methanol extract of the dried mushroom sample was used to perform the analysis to determine the antioxidant activity and content of total phenolic compounds. It was found that L. sanguifluus methanol extract contained 3.677±0.207 mg GAE/g DW phenolic compounds and exhibited radical scavenging activities as 211.652±0.537 mg TE/g DW against DPPH and 73.389±1.003 mg TE/g DW against ABTS. L. sanguifluus methanol extract also had metal ion reducing capabilities with ferric reducing and cupric reducing antioxidant capacities of 191.903±0.659 mg AAE/g DW and 379.598±7.527 mg TE/g DW, respectively. The GC-MS analysis of the hexane extract of the mushroom sample revealed that palmitic acid, linoleic acid, oleic acid and stearic acid were the predominant fatty acids found in L. sanguifluus.
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Aljassim, Zainab G., Haitham M. Kadhim, and Abbas S. Al-mizraqchi. "Evaluation of Antimicrobial Activity of Pleurotus ostreatus on Selected Multi-Drug Resistant Bacteria and Fungus." Sumer 2 8, CSS 2 (2023): 1–10. http://dx.doi.org/10.21931/rb/css/2023.08.02.72.

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Pleurotus ostreatus, or oyster mushroom, is a common edible wild mushroom characterized by its high nutritional values and promising diverse biological activities. It contains many bioactive components which have been found to possess several therapeutic functions. Because of the rising threat of treating serious and resistant infections, there is a developing need to discover new treatment strategies and compounds that can effectively eradicate infections. This study aims to evaluate and measure the antimicrobial activity and the minimum bactericidal concentration (MBC) and Minimum fungicidal concentration (MFC) of Pleurotus ostreatus methanol and aqueous crude extracts on Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Escherichia coli, Mutans Streptococci, Lactobacillus, and Candida albicans. The antimicrobial activity of mushroom extracts was evaluated against ten purified microbial isolates using agar disc diffusion assay and minimum bactericidal and fungicidal concentration assay. The results show that P. ostreatus methanol and aqueous crude extracts revealed antibacterial activity against the tested microorganisms and formed growth inhibition zones. Methanol crude extract shows more potent growth inhibition than the aqueous extract and in lower concentrations. This study shows that the tested oyster mushroom extracts have antimicrobial effects on different pathogens. Methanol crude extract of P. ostreatus revealed more powerful antibacterial and antifungal activity than the aqueous crude extract. Keywords: Oyster mushroom, Antimicrobial activity, Methanol crude extract.
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Tung, Yu-Tang, Chun-Hsu Pan, Yi-Wen Chien, and Hui-Yu Huang. "Edible Mushrooms: Novel Medicinal Agents to Combat Metabolic Syndrome and Associated Diseases." Current Pharmaceutical Design 26, no. 39 (2020): 4970–81. http://dx.doi.org/10.2174/1381612826666200831151316.

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Metabolic syndrome is an aggregation of conditions and associated with an increased risk of developing diabetes, obesity and cardiovascular diseases (CVD). Edible mushrooms are widely consumed in many countries and are valuable components of the diet because of their attractive taste, aroma, and nutritional value. Medicinal mushrooms are higher fungi with additional nutraceutical attributes having low-fat content and a transisomer of unsaturated fatty acids along with high fiber content, biologically active compounds such as polysaccharides or polysaccharide β-glucans, alkaloids, steroids, polyphenols and terpenoids. In vitro experiments, animal models, and even human studies have demonstrated not only fresh edible mushroom but also mushroom extract that has great therapeutic applications in human health as they possess many properties such as antiobesity, cardioprotective and anti-diabetic effect. They are considered as the unmatched source of healthy foods and drugs. The focus of this report was to provide a concise and complete review of the novel medicinal properties of fresh or dry mushroom and extracts, fruiting body or mycelium and its extracts, fiber, polysaccharides, beta-glucan, triterpenes, fucoidan, ergothioneine from edible mushrooms that may help to prevent or treat metabolic syndrome and associated diseases.
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Seetaha, Supaphorn, Siriluk Ratanabunyong, Lueacha Tabtimmai, Kiattawee Choowongkomon, Jatuporn Rattanasrisomporn, and Khuanjarat Choengpanya. "Anti-feline immunodeficiency virus reverse transcriptase properties of some medicinal and edible mushrooms." Veterinary World 13, no. 9 (2020): 1798–806. http://dx.doi.org/10.14202/vetworld.2020.1798-1806.

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Background and Aim: Feline immunodeficiency virus (FIV) causes AIDS-like symptoms in domestic and wild cats. Treatment of infected cats has been performed using human anti-HIV drugs, which showed some limitations. This study aimed to determine the anti-FIV potential of some mushrooms. Materials and Methods: A total of 17 medicinal and edible mushrooms were screened to find their inhibitory effect against FIV reverse transcriptase (FIV-RT). Three solvents, water, ethanol, and hexane, were used to prepare crude mushroom extracts. Fluorescence spectroscopy was used to perform relative inhibition and 50% inhibitory concentrations (IC50) studies. Results: The ethanol extract from dried fruiting bodies of Inonotus obliquus showed the strongest inhibition with an IC50 value of 0.80±0.16 μg/mL. The hexane extract from dried mycelium of I. obliquus and ethanol and water extracts from fresh fruit bodies of Phellinus igniarius also exhibited strong activities with the IC50 values of 1.22±0.20, 4.33±0.39, and 6.24±1.42 μg/mL, respectively. The ethanol extract from fresh fruiting bodies of Cordyceps sinensis, hexane extracts from dried mycelium of I. obliquus, ethanol extracts of Ganoderma lucidum, hexane extracts of fresh fruiting bodies of Morchella esculenta, and fresh fruiting bodies of C. sinensis showed moderate anti-FIV-RT activities with IC50 values of 29.73±12.39, 49.97±11.86, 65.37±14.14, 77.59±8.31, and 81.41±17.10 μg/mL, respectively. These mushroom extracts show anti-FIV potential. Conclusion: The extracts from I. obliquus, P. igniarius, C. sinensis, and M. esculenta showed potential anti-FIV activity.
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Adenuga, Victoria Oluwapelumi, Soji Fakoya, Joseph Adaviruku Sanni, Akinola Adenuga, Ore-ofe Oluwatoyin Adenuga, and Oluwaferanmi Timileyhin Ajayi. "Antimicrobial Activities of Oyster Mushroom (Plerotous ostreatus), Garlic (Allium sativum), and Ginger Extracts (Zingiber officinale) Against Some Clinical Isolates." Biology, Medicine, & Natural Product Chemistry 13, no. 2 (2024): 389–95. http://dx.doi.org/10.14421/biomedich.2024.132.389-395.

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The prevalence of multiple drug resistance among human pathogenic bacteria necessitates a continual search for new antimicrobial medicines, particularly among plants that are frequently farmed or naturally found in our surroundings. The study aims to investigate the inhibitory effect of garlic, ginger, and mushroom extracts at different concentrations against some clinical isolates. Anti-bacterial components from mushrooms, garlic, and ginger were extracted with hot water, cold water, ethanol, and acetone, and their anti-bacterial activity was determined using the agar well diffusion method. Zones of inhibition were observed primarily on hot water extracts of mushrooms (Pleurotus ostreatus) on culture plates inoculated with Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia at 370C for 24 hours. The cold water extracts of the mushroom (Pleurotus ostreatus) gave the highest zone of inhibition of 14.0±1.0mm when used against S. aureus. For spices, the cold water extracts yielded the highest zones of inhibition of 22.0±1.0mm followed by 16.0±1.0mm as observed with ginger. The results obtained have shown clearly that the mushrooms (Pleurotus ostreatus), garlic (Allium sativum), and ginger (Zingiber officinale) extracts contain phytochemicals with some antimicrobial activities. The water extracts of the mushrooms and spices showed broad-spectrum antimicrobial activity much more than ethanol and acetone extracts. The antimicrobial activities of mushroom and garlic extracts were highly effective against the bacterial pathogens studied. However, the antimicrobial activity of the ginger extract was poor. To address the multi-drug resistance to antibiotics, I recommend: that bioactive compounds found in mushrooms, ginger, and garlic be patented and used as alternative antimicrobials.
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Vinay, Kumar, and Yadav Udit. "SCREENING OF ANTIFUNGAL ACTIVITY OF PLEUROTUS OSTREATUS AND AGARICUS BISPORUS." Biolife 2, no. 3 (2022): 918–23. https://doi.org/10.5281/zenodo.7224733.

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<strong>ABSTRACT</strong> The fruiting body powder of <em>Pleurotus ostreatus</em> (oyster mushroom) and <em>Agaricus bisporus</em> (button mushroom) was extracted with ethanol by using the agar well diffusion technique, the extract were tested for their antifungal activity against six fungi belonging to 5 genera viz. <em>Aspergillus flavus, Aspergillus fumigatous, Penicillium chrysogenum</em>, <em>Sporotricum carnis, Humicola grisea</em> <em>and Thermoascus aurantiacus</em>. The extract of <em>Pleurotus ostreatus</em> showed the maximum inhibition against <em>Penicillium chrysogenum</em> and minimum against <em>Aspergillus flavus</em>. The extract of <em>Agaricus bisporus</em> showed the maximum inhibition against <em>Humicola grisea</em> and minimum against same fungi <em>Aspergillus flavu</em> <strong>Key Words:-</strong> Antifungal activity, Fruiting Body, <em>Pleurotus ostreatus , Agaricus Bisporus</em>, ethanol extract <strong>REFERENCES</strong> Amuneke EH, Dike KS, and Ogbulie JN, 2011. Cultivation of Pleurotus ostreatus: An edible mushroom from agro base waste products. J. Microbiol. Biotech. Res., Vol. 1 (3):1-14. Aneja KR, 2001. Experiments in Microbiology. Plant pathology and Biotechnology, Newage International Publishers. Vol 4:157-162. Barros L, Calhella RC and Vaz JA, 2007. Antimicrobial activity and bioactive compounds of Portugese wild edible mushrooms methanolic extracts. European Food Research and Technology. 225: 151-156. Bender S, Lonergan GT, Backhaus J, Dumitrache A andBaker WL, 2001. The antibacterial activity of the edible and medicinal mushroom Lentinus edodes (Berk.) Sing. Int. J. Med. Mushrooms 3: 118. Benedict RG and Brady LR, 1972. Antimicrobial activity of mushroom metabolites. J. Pharm. Sci. 61: 1820-21. Chang ST and Miles Philip G, 2004. Mushrooms Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. 2nd Edn.CRC Press.USA. Cohen R, Persky L and Hadar Y, 2002. Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl. Microbiol. Biotechnol. 58: 582-594. Demirhan A, Yesil OF, Yildiz A and Gul K, 2007. A research on antimicrobial activity of some macrofungi species. Science and Engineering Journal of Firat University. 19: 425-433. Gbolagade JS and Fasidi IO, 2005. Antimicrobial activities of some selectedNigerian mushrooms. 8: 83-87. Gilman JC, 2001. A Manual of Soil fungi. Biotech Books, 2nd Indian edition, Delhi. Jonathan SG and Fasidi JO, 2003. Antimicrobial activities of two Nigerian edible macro-fungi Lycoperdon pusilum (Bat. Ex) and Lycoperdon giganteum (Pers). African.J.Biomed.Res. 6: 85-90. Nagamani A, Kunwar IK and Manoharachary C, 2006. Hand book of soil fungi. I.K. International Pvt. Ltd. Nehra Kiran, Meenakshi, Kumar Mukesh and Yadav Ajay, 2012. Evaluation of antimicrobial potential of fruiting body extracts of Pleurotus ostreatus (oyster mushroom). International Journal of Microbial Resource Technology.Vol.1,4-2278 &ndash; 3822. Ngai PHK and Ng TB, 2004. A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides 25: 11-17. Richa, Shikha Sharma and M.L. Sharma Phytochemical Investigations And Anatomical Study Of Three Species Of Sida. Biolife. 2(2):622-629. Solak MH, Kalmis E, Saglam H and Kalyoncu F, 2006. Antimicrobial activity of two wild mushrooms Clitocybe alexandri (Gill.) Konr. and Rhizopogon roseolus (Corda) T.M. Fries collected from Turkey. Phytotherapy Research. 20: 1085-1087. Tochikura TS, Nakashima H, Ohashi Y and Yamamoto N, 1988. Inhibition (in- vitro) of replication and of the cytopathic effect of human immunodeficiency virus by an extract of the culture medium of Lentinus edodes mycelia. Med. Microbiol. Imm. 177: 235-244. Turkoglu A, Kivrak I, Mercan N, Duru ME, Gezer K and H. Turkoglu H, 2006. Antioxidant and antimicrobial activities of Morchella conica. Pers. African Journal of Biotechnol. 5: 1146-1150. Waksman SA, 1922. A method of counting the number of fungi in the soil. J. Bact. 7: 339-341. Wang HX and Ng TB, 2000. Isolation of a novel ubiquitin-like protein from Pleurotus ostreatus mushroom with anti-humanimmunodeficiency virus, translation-inhibitory and ribonuclease activities. . 276: 587-93. Wang HX, Ng TB and Liu QH, 2004. Alveolarin, a novel antifungal polypeptide from the wild mushroom Polyporus alveolaris. Peptides 25: 693&ndash;696. Wang J, Wang HX and Ng TB, 2007. A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa. Peptides 28: 560-65. &nbsp;
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Chu, Minghang, Rana Dildar Khan, Ying Zhou, et al. "LC-ESI-QTOF-MS/MS Characterization of Phenolic Compounds in Common Commercial Mushrooms and Their Potential Antioxidant Activities." Processes 11, no. 6 (2023): 1711. http://dx.doi.org/10.3390/pr11061711.

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Mushrooms have a long history of use as food and medicine. They are rich in various nutrients and bioactive compounds, particularly phenolic compounds. In this study, ten mushroom species were selected, and solvent extraction using 80% ethanol was used to extract phenolic compounds. Total phenolic content (TPC), total flavonoid content (TFC) and total condensed tannin content (TCT) were measured to evaluate phenolic content in different mushroom varieties. In the mushroom varieties tested, brown portobello mushroom had the highest TPC (396.78 ± 3.12 µg GAE/g), white cup mushroom exhibited the highest TFC (275.17 ± 9.40 μg CE/g), and shiitake mushroom presented the highest TCT (13.80 ± 0.21 µg QE/g). Antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2,2′-azino-bis-3ethylbenzothiazoline-6-sulfonic acid (ABTS) and total antioxidant capacity (TAC) assays. The highest DPPH free radical scavenging ability was found in white cup mushroom (730.14 ± 55.06 µg AAE/g), while the greatest iron-reducing ability (FRAP) was recorded for shiitake mushroom (165.32 ± 10.21 μg AAE/g). Additionally, Swiss brown mushroom showed the highest ABTS antioxidant capacity (321.31 ± 5.7 μg AAE/g), and the maximum TAC value was found in shiitake mushroom (24.52 ± 1.2 μg AAE/g). These results highlight that most of the mushroom varieties studied showed high phenolic contents and demonstrated strong antioxidant activity, with shiitake mushrooms standing out due to their high TCT and FRAP values, and the highest TAC value among the varieties studied. In addition, LC-ESI-QTOF-MS/MS was used to characterize the mushroom samples, and tentatively identified a total of 22 phenolic compounds, including 11 flavonoids, 4 lignans, 3 phenolic acids, 2 stilbenes and 2 other phenolic compounds in all mushroom samples. The research results of this study showed that mushrooms are a good source of phenolic compounds with strong antioxidant potential. The results can provide a scientific basis for the development of mushroom extracts in functional food, health products, and other industries.
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Pala, Shauket Ahmed, Abdul Hamid Wani, and Bashir Ahmad Ganai. "Antimicrobial potential of some wild Macromycetes collected from Kashmir Himalayas." Plant Science Today 6, no. 2 (2019): 137–46. http://dx.doi.org/10.14719/pst.2019.6.2.503.

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Alarming increase in microbial resistance to existing synthetic commercial antibiotics forced scientists to search for new antimicrobials from various alternative sources. The present study carried out during the year 2014-2015, presents the antimicrobial potential of some mushroom extracts against some commonly found pathogenic bacterial and fungal microbes. During the study four mushroom species, viz. Lentinus tigrinus (Bull.) Fr., Fomitopsis pinicola (Sw.) P.Karst, Inonotus hispidus (Bull.) P.Karst and Ramaria formosa (Pers.) Quel. were evaluated for their antimicrobial activity against both gram positive (Bacillus subtilis and Staphylococcus aureus), gram negative (Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae and Pseudomonas aeruginosa) and fungi (Saccharomyces cerevisiae Saccharomyces cerevisiae, Candida albicans, Penicillium chrysogenum and Aspergillus fumigates). The results revealed that ethyl acetate and methanolic extract of all the mushroom extracts showed significant antimicrobial activity against most of the bacterial and fungal microbes. However, the aqueous extract of these mushrooms was found either lacking or conferring insignificant antimicrobial activity. The ethyl acetate extracts of Ramaria formosa and Lentinus tigrinus produced more promising results against the bacterial microbes than fungal counterparts. Both ethyl acetate and methanolic extracts of Fomitopsis pinicola and Inonotus hispidus exhibited strong antimicrobial activity against the selected set of microbes. The antibacterial and antifungal activity exhibited by Fomitopsis pinicola at the concentration 150mg/ml was almost parallel to 10µg gentamycin and 50µg nystatin respectively. Therefore, Fomitopsis pinicola signifies as one of the promising mushroom species possessing strong antimicrobial activity against broad spectrum of microbes.
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Korkmaz, Ali İmran, Celal Bal, Tetiana Krupodorova, Mehmet Ali Yüzbaşıoğlu, Beste Gizem Özbey Sarıdoğan, and Mustafa Sevindik. "Some biological activities and element levels of Lycoperdon pratense." Prospects in Pharmaceutical Sciences 22, no. 2 (2024): 39–44. http://dx.doi.org/10.56782/pps.189.

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Mushrooms are natural products with many biological activities. In this study, antioxidant, antimicrobial, anticholinesterase activities and element levels of Lycoperdon pratense Pers. were determined. In this context, the mushroom was extracted with ethanol in a soxhlet device. Antioxidant potential was determined using Rel assay kits. Antimicrobial activity was determined using the agar dilution method. Antialzheimer activity was determined by acetyl- (AChE) and butyrylcholinesterase (BChE) potentials. The levels of elements accumulated in the mushroom were scanned using the wet digestion method. As a result of the analysis, the TAS value of the mushroom was measured as 2.589 ± 0.118 mmol Trolox equiv./L, the TOS value was 10.360 ± 0.197 µmol H2O2 equiv./L and the OSI value was 0.401±0.013. The mushroom extract was found to have high activity against bacteria. It was determined that the extract had an effect against microorganisms at concentrations between 50-400 μg/mL. It was determined that the anti-AChE IC50 value of the mushroom extract was 14.48 ± 0.80 μg/mL and the anti-BChE IC50 value was 23.10±1.21 μg/mL. It is also thought that it can be used as an indicator in terms of element levels within the mushroom. As a result, it was determined that the mushroom has antioxidant, antimicrobial and antiallergic potential.
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Canpolat, Şükrü, Ilgaz Akata, Yasemin İşlek, Elif Canpolat, and Cemil İşlek. "Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde." Turkish Journal of Agriculture - Food Science and Technology 9, no. 10 (2021): 1915–20. http://dx.doi.org/10.24925/turjaf.v9i10.1915-1920.4642.

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Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract.
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Jakopovic, Boris, Nada Oršolić, and Ivan Jakopovich. "Proteomic Research on the Antitumor Properties of Medicinal Mushrooms." Molecules 26, no. 21 (2021): 6708. http://dx.doi.org/10.3390/molecules26216708.

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Medicinal mushrooms are increasingly being recognized as an important therapeutic modality in complementary oncology. Until now, more than 800 mushroom species have been known to possess significant pharmacological properties, of which antitumor and immunomodulatory properties have been the most researched. Besides a number of medicinal mushroom preparations being used as dietary supplements and nutraceuticals, several isolates from mushrooms have been used as official antitumor drugs in clinical settings for several decades. Various proteomic approaches allow for the identification of a large number of differentially regulated proteins serendipitously, thereby providing an important platform for a discovery of new potential therapeutic targets and approaches as well as biomarkers of malignant disease. This review is focused on the current state of proteomic research into antitumor mechanisms of some of the most researched medicinal mushroom species, including Phellinus linteus, Ganoderma lucidum, Auricularia auricula, Agrocybe aegerita, Grifola frondosa, and Lentinus edodes, as whole body extracts or various isolates, as well as of complex extract mixtures.
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Laksono, Rommy Andhika, Fawzy Muhamad Bayfurqon, and Miftakhul Bakhrir R.K. "Uji Efektivitas Berbagai Konsentrasi Jenis Nutrisi Alternatif Terhadap Produksi Jamur Tiram Putih (Pleurotus ostreatus) di Kabupten Karawang." Paspalum: Jurnal Ilmiah Pertanian 6, no. 1 (2018): 32. http://dx.doi.org/10.35138/paspalum.v6i1.75.

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In the development of modern agriculture based on location specification agriculture, to increase the production of white oyster mushrooms, requires able to utilize alternative natural resources that are easy to obtain and efficient.The research was conducted with the aim to study and get the concentration of alternative nutrient types that can provide the highest production of white oyster mushroom in Karawang regency. Based on the purpose, this research is verifikatif.The method used is experimental method and experiment design used is single factor randomized block design (RAK) consisting of 17 treatments in 3 repetitions : A (100% Aquades Concentration); B (20% Leri Water Concentration); C (40% Leri Water Concentration); D (60% Leri Water Concentration); E (80% Leri Water Concentration); F (20% Potato Skin Extract Concentration); G (40% Potato Skin Extract Concentration); H (60% Potato Skin Extract Concentration); I (80% Potato Skin Extract Concentration); J (20% Bean Sprouts Extract Concentration); K (40% Bean Sprouts Extract Concentration); L (60% Bean Sprouts Extract Concentration); M (80% Bean Sprouts Extract Concentration); N (20% Old Coconut Water Concentration); O (40% Old Coconut Water Concentration); P (60% Old Coconut Water Concentration); Q (80% Old Coconut Water Concentration). The effect of treatment was analyzed by F test of 5% level and if significant, then to know the best treatment was continued with Duncan Multiple Range Test at 5% real level.The results achieved from this research is the application of various types of concentrations of alternative nutrients have a real effect on total clumps per baglog, total hood per clumps, maximum hood diameter per baglog, harvest intensity per baglog, dry weight of mushrooms per baglog, and fresh weight of mushrooms per baglog white oyster mushroom in Karawang regency. 80% concentration of leri water is able to give the highest growth and yield of white oyster mushroom in Karawang regency, with fresh weight of mushroom reach 142,35 gr per baglog per week or 0,85 tons per 1000 baglog in one plant period (6 MSA).
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Chuerduangphui, Jureeporn. "Potential water-based extracts from fresh and dried auricularia auricula-judaeon the inhibitory effect of human papillomavirus type 16 and cervical cancer cell line in caski." Journal of medical pharmaceutical and allied sciences 11, no. 6 (2022): 5469–75. http://dx.doi.org/10.55522/jmpas.v11i6.4183.

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Auricularia auricula-judae, also called the wood ear mushroom, is a well-known edible mushroom in various countries. Notably, the extracts of this mushroom inhibited the growth and expression of human papillomavirus (HPV) oncogene in the HPV-positive cervical cancer cell line. However, there has been no reported comparison of the effect of the extracts from fresh and dried A. auricula-judae on anti-HPV16 in CaSki cell lines. Thus, this study investigated the effect of the water-based extracts from fresh and dried A. auricula-judae at 100oC on anti-HPV16 pseudovirus infection and anti-cancer in CaSki as well as the expression of viral oncogenes and cellular genes. HPV16 pseudovirus was produced and used for anti-viral infection assay in pre-attachment and adsorption steps in treated 293FT cells. Cell apoptosis, cell proliferation, cell migration and colony-forming assay were investigated in treated CaSki cells. In addition, mRNA expression levels of HPV16E6/E7, BCL2, BAK and CASP3 were determined using quantitative real time polymerase chain reaction. The results indicated that the extract from dried mushroom caused higher cytotoxicity than fresh mushroom. Notably, the extracts from the dried mushroom more effectively inhibited the infection of HPV16 pseudovirus in 293FT cells than did fresh mushroom. Furthermore, it significantly decreased HPV16E6/E7 and BCL2 and increased BAK and CASP3 expression, in contrast to the untreated and water-treated CaSki cells, but not in the extract derived from fresh mushroom. The main conclusion was that the extract from dried mushroom more effectively inhibited growth of CaSki and HPV16-pseudovirus infection than from fresh mushroom.
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Shafiq, Shatha Ali, and Rasha Salam Sahib. "Antagonistic Activity of Agaricuscampestris mushroom Extracts Against some Human Pathogenic Bacterial Species." Asian Journal of Pharmaceutical Research and Development 10, no. 2 (2022): 13–16. http://dx.doi.org/10.22270/ajprd.v10i2.1115.

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The present study focused on the evaluation of Agaricuscampestris mushroom extracts activity against four clinical isolates Escherichia coli; Pseudomonas aerginosa; Staphylococcus haemolyticus and Staphylococcus aureus. Agaricuscampestris mushroom extracts varied in their antimicrobial activity. The intensity of the antimicrobial effect is dependent upon the solvent , concentration of the mushroom extract and the organism being tested against .Therefore, The hot water and ethanolic extract 96%showed maximum inhibition at high concentrations specially at 500 mg/ml concentration( 17, 16, 19, 16 ) mm and ( 20,21,19,20) against E. coli , Pseudomonas aerginosa; Staphylococcus aureus and Staphylococcus haemolyticus respectively compared with methanolic extract . The ethyl acetate extract of the powder mushroom of Agaricuscampestris was analyzed to 9 chemical compounds belonged to fatty acids and their derivatives and included :-Nonadecane, Pentacosane, Octadecane, Heptadecane, Heneicosane, Hexadecane, 2,6,10,14-tetramethyl, Butanoic acid, 3,7-dimethyl-6-octenyl ester, Phthalic acid mono-2-ethylhexyl ester ,, Octanoic acid, heptadecyl ester.&#x0D;
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Svilpe, Elīna, and Nataļja Matjuškova. "Influence of shiitake mushroom Lentinula edodes on reproduction of Drosophila melanogaster." Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. 64, no. 5-6 (2010): 223–28. http://dx.doi.org/10.2478/v10046-010-0008-2.

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Influence of shiitake mushroom Lentinula edodes on reproduction of Drosophila melanogaster Shiitake mushroom Lentinula edodes is an edible basidiomycete cultivated worldwide, with high nutritious value and diverse biological activity. There has been an increase in its use as food supplement. Influence of shiitake mushroom extract on the reproductive function and development in an object fruit fly Drosophila melanogaster is described in this research. Fruit flies were maintained on banana medium with or without supplementation of shiitake mushroom's extract, standardized per amount of crude polysaccharides. Shiitake extract supplement, 0.030% and 0.015% crude polysaccharides per volume, induced a statistically significant increase in total number of pupae and flies, and promoted pupae viability. Adult drosophilae males, which received shiitake extract supplement for seven days, had a statistically significant reduction in copulation latency, while thirty day exposure to extract promoted a statistically higher rate of mated flies. Females showed an increase in number of mated flies and reduction in copulation latency post seven day feeding period and had no significant effect on mating ability and fertility post thirty days. Further studies are planned to identify the biologically active components of shiitake mushroom hot water extract and to characterize their effects on reproductive function.
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44

Ihayere, C. A., and J. A. Okhuoya. "Phytochemical analysis of cultivated medicinal mushroom- Ganoderma sp." Nigerian Journal of Botany 35, no. 1 (2022): 11–18. http://dx.doi.org/10.4314/njbot.v35i1.2.

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The aim of this study was to evaluate the phytochemical contents of indigenous cultivated Ganoderma sp. (Curt.) P. Karst. in comparison to other mushrooms found in literature. The cultivated indigenous medicinal mushroom (Ganoderma sp.) can be available all-year round, as compared with the wild or imported/foreign Ganoderma sp. which are scarce, seasonal and expensive. Phytochemical analysis was carried out using Methanol, Ethanol, Dichloromethane and Aqueous extracts. Preliminary tests indicated the presence of secondary metabolites (alkaloids, tannins, phenols, terpenoids, flavonoids, steroids) and high carbohydrate contents in the cultivated Ganoderma fruiting bodies. Results of the quantitative analysis showed the highest value of phenol in aqueous extract, with the least value in Dichloromethane extract for flavonoids. The results obtained from this study reveals that the cultivated indigenous medicinal mushroom, Ganoderma sp. is a potential source of secondary metabolites which are beneficial as nutraceuticals in industries and pharmaceuticals.
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Sevindik, Mustafa. "Wild Edible Mushroom Cantharellus cibarius as a Natural Antioxidant Food." Turkish Journal of Agriculture - Food Science and Technology 7, no. 9 (2019): 1377–81. http://dx.doi.org/10.24925/turjaf.v7i9.1377-1381.2644.

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In this study, DPPH free radical activity, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of wild edible mushroom Cantharellus cibarius Fr. collected from Antalya (Turkey) province were determined. Ethanol (EtOH), methanol (MeOH) and dichloromethane (DCM) extracts of C. cibarius mushroom were obtained using soxhlet apparatus. TAS, TOS and OSI values were determined by using Rel Assay kits. Free radical scavenging activity was determined using DPPH method. As a result of the studies, TAS value of the mushroom was found as 5.268±0.059 mmol/L, TOS value was 6.380±0.256 μmol/L and OSI value was 0.121±0.005. DPPH free radical activity was determined as EtOH extract 70.52±0.50, MeOH extract 64.34±1.54 and DCM extract 61.72±0.59 in 1 mg/mL extract concentration of mushroom. As a result, edible C. cibarius mushroom could be a natural antioxidant source.
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46

N., Tamilselvan, and Rajesh K. "Antimicrobial Efficacy of Medicinal Mushroom Ganoderma Lucidum." International Journal of Trend in Scientific Research and Development 3, no. 3 (2019): 1798–800. https://doi.org/10.31142/ijtsrd23522.

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Mushroom has multi beneficial effects for human welfare. The Medicinal mushrooms are widely used as traditional medicinal components for the treatment of various diseases and related health problems. Most of the medicinal extracts from mushroom are different forms of polysaccharides which strengthens the human immune system with no side effect. Medicinal mushroom Ganoderma species investigation has focused on the biological response of microorganisms. The antimicrobial efficacy of various solvent extracts 20g ml of Ganoderma lucidum was treated against five different bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa and Klebsiella sp and four different fungal pathogens like Aspergillus niger, Fussarium sp, Penicillum sp and Candida sp. Ethanol extract exhibited maximum antibacterial and antifungal activity, while the most susceptible bacterium observed was Staphylococcus aureus, Pseudomonas aeruginosa and fungi was fusarium sp. respectively. N. Tamilselvan | K. Rajesh &quot;Antimicrobial Efficacy of Medicinal Mushroom Ganoderma Lucidum&quot; Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd23522.pdf
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Bristy, Anika Tabassum, Tairin Islam, Rezwana Ahmed, Jumana Hossain, Hasan Mahmud Reza, and Preeti Jain. "Evaluation of Total Phenolic Content, HPLC Analysis, and Antioxidant Potential of Three Local Varieties of Mushroom: A Comparative Study." International Journal of Food Science 2022 (October 19, 2022): 1–11. http://dx.doi.org/10.1155/2022/3834936.

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Functional foods such as mushrooms are rich in polyphenolic compounds and secondary metabolites with health-promoting properties such as antioxidant, antimicrobial, antidiabetic and immunostimulatory effects. The present study is aimed to investigate the ethanolic extracts of three varieties of mushrooms, namely, G. lucidum, G. tropicum, and C. indica grown in Bangladesh for phenolic and flavonoid content and their antioxidant properties. Moreover, the phenolic composition of the extracts was analyzed by using the HPLC-DAD system. G. lucidum extract exhibited the highest antioxidant potential as evidenced by its lowest IC50 value in all the tested assay models ( 40.44 ± 2.09 μg/mL, 151.32 ± 0.35 μg/mL, 137.89 ± 1.85 μg/mL in DPPH, H2O2, and NO scavenging assay, respectively) along with the highest phenolic content ( 81.34 ± 0.68 GAE g-1 extract). G. tropicum and C. indica extracts also showed significant antioxidant properties and a good amount of phenolic content, 52.16 ± 0.25 GAE g-1 extract, and 47.1 ± 0.26 GAE g-1 extract, respectively. The scavenging activity increased with the increasing concentration of extracts in all cases. The total phenolic content of the ethanolic extracts of mushroom species was highly correlated with antioxidant effects with Pearson’s correlation coefficient ( r ) values ranging from 0.8883–0.9851. The α-amylase inhibitory and antibacterial activity of G. lucidum was evaluated by using 3,5-dinitrosalicylic acid and disc diffusion method, respectively. The maximum inhibitory activity recorded against α-amylase was 70.98 ± 0.042 % at a concentration of 500 μg/mL. G. lucidum extract exhibited the highest antibacterial activity against Pseudomonas aeruginosa with 23.00 ± 1.00 mm clear zone of inhibition and an MIC value of 3.5 mg/mL. The results indicate that the mushroom species tested in this study could serve as a potential source of natural antioxidants in the development of nutraceuticals and herbal drugs for the management of oxidative stress-associated diseases as well as infectious diseases.
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FERNANDEZ, PABLO S., MARIA J. OCIO, TOMAS SANCHEZ, and ANTONIO MARTINEZ. "Thermal Resistance of Bacillus stearothermophilus Spores Heated in Acidified Mushroom Extract." Journal of Food Protection 57, no. 1 (1994): 37–41. http://dx.doi.org/10.4315/0362-028x-57.1.37.

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The thermal resistance of Bacillus stearothermophilus spores was studied in bidistilled water as the reference medium, mushroom extract and acidified mushroom extract. Citric acid and glucono-δ-lactone were used as acidulants. Results indicated that mushroom extract affects the heat resistance of spores; D values were lower than for those in bidistilled water. The pH effect was lower with higher treatment temperatures. Acidification reduced the thermal resistance of spores, the reduction being similar for both types of acidulants, and in general it also had the effect of increasing the z values. It has been confirmed that acidification of the canned mushrooms could actually help to control the thermophilic spoilage. This acidification could also be obtained by the use of glucono-δ-lactone, which has been shown to be as effective as citric acid in reducing the heat resistance of spores.
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Hossen, Md Sakib, Md Maruf Billah Prince, E. M. Tanvir, et al. "Ganoderma lucidum and Auricularia polytricha Mushrooms Protect against Carbofuran-Induced Toxicity in Rats." Evidence-Based Complementary and Alternative Medicine 2018 (2018): 1–13. http://dx.doi.org/10.1155/2018/6254929.

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The current study aimed to investigate the ameliorative effects of two types of mushrooms, Ganoderma lucidum (GL) and Auricularia polytricha (AP), against carbofuran- (CF) induced toxicity in rats. Male Wistar rats (n=42) were divided into six equal groups. The rats in the negative control group received oral administration of CF at 1 mg/kg with the normal diet for 28 days. The treatment groups received oral administration of ethanolic extract of GL or AP at 100 mg/kg followed by coadministration of CF at 1 mg/kg with the normal diet for the same experimental period, respectively. In the CF alone treated group, there were significant decreases in the erythrocytic and thrombocytic indices but increases in the concentrations of the total leukocytes, including the agranulocytes. A significant increase in all of the liver function biomarkers except albumin, in lipid profiles except high-density lipoprotein, and in the kidney function markers occurred in the negative control group compared to the rats of the normal control and positive control groups. The coadministration of mushroom extracts significantly ameliorated the toxic effects of the CF. The GL mushroom extract was more efficacious than that of the AP mushroom, possibly due to the presence of high levels of phenolic compounds and other antioxidants in the GL mushroom.
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Sakul, S., S. Komansilan, M. Tamasoleng, D. Rumondor, and R. Hadju. "Antioxidant activity and viability Lactobacillus acidophilus synbiotic yogurt with the addition of white oyster mushroom (Pleurotus ostreatus) extract." IOP Conference Series: Earth and Environmental Science 1341, no. 1 (2024): 012039. http://dx.doi.org/10.1088/1755-1315/1341/1/012039.

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Abstract The aim of this study was to evaluate the antioxidant activity, Lactobacillus acidophilus bacterial survival, cholesterol levels, and total lactic acid bacteria of synbiotic yogurt added to Pleurotus ostreatus white oyster mushroom extract. Synbiotic yogurt is a fermented milk product that is prepared by adding probiotic lactic acid bacteria, such as Streptococcus thermophillus, Lactobacillus bulgaricus, and Lactobacillus acidophilus. White oyster mushrooms have minerals and dietary fiber called beta-glucan that can function as prebiotics. White oyster mushroom extract concentrations were utilized as treatments with T0 = 0%, T1 = 2%, T2 = 4%, T3 = 6%, and T4 = 8% in the full randomized design (RAL) used in this study. Repeated four times for each treatment. The outcomes demonstrated a very significant impact (P&lt; 0.01) of the synbiotic yogurt with the inclusion of white oyster mushroom extract on the antioxidant activity, viability of Lactobacillus acidophilus bacteria, cholesterol levels, and total lactic acid bacteria. The findings of this study suggest that a larger concentration of white oyster mushroom extract has significant antioxidant activity, increases the viability of all lactic acid bacteria, including Lactobacillus acidophylus, and lowers total cholesterol.
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