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Dissertations / Theses on the topic 'Mutagenesis'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Martin, Stephen Lewis. "Novel methods for mutagenesis." Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302789.

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2

Ennis, Don Gregory. "Genetics of SOS mutagenesis." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184602.

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Previous genetic evidence suggested that RecA was required in SOS mutagenesis for its regulatory role and perhaps some other nonregulatory role (Mount, 1977; Blanco et al., 1982). I undertook a genetic study which confirmed the above studies and provided further evidence that RecA protein appeared to have a dual "role in mutagenesis; first, the cleavage of LexA repressor for the derepression of specific SOS genes and second, one or more additional role(s). For these studies a new phage mutagenesis assay was developed which allows rapid scoring of SOS mutagenesis in a large number of host mutan

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3

Porto, Marília de Paula [UNESP]. "Ação moduladora do citral e eugenol em eventos genéticos em magrófagos murinos in vitro." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/92460.

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Made available in DSpace on 2014-06-11T19:26:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-27Bitstream added on 2014-06-13T19:53:56Z : No. of bitstreams: 1 porto_mp_me_botib.pdf: 2132236 bytes, checksum: b1dd297e7ebc3769eafbc4dbcaa5705a (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>Devido a propriedades terapêuticas, várias plantas e seus constituintes químicos vêm sendo muitas vezes utilizados como o prime

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4

Porto, Marília de Paula. "Ação moduladora do citral e eugenol em eventos genéticos em magrófagos murinos in vitro /." Botucatu : [s.n.], 2012. http://hdl.handle.net/11449/92460.

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Orientador: Daisy Maria Fávero Salvadori<br>Coorientador: Glenda Nicioli da Silva<br>Banca: Luís Fernando Barbisan<br>Banca: Denise Crispim Tavares<br>Resumo: Devido a propriedades terapêuticas, várias plantas e seus constituintes químicos vêm sendo muitas vezes utilizados como o primeiro recurso para o tratamento de diversas doenças. Nesse contexto, compostos isolados de plantas têm sido alvos de inúmeros estudos que avaliam, além da atividade, seus possíveis mecanismos de ação. Dentre os compostos com potencial quimioprotetor, o citral e o eugenol merecem atenção devido suas estruturas quími

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5

Silva, Ana Carolina Buzinari da [UNESP]. "Análise de uma biblioteca de mutantes de Xanthomonas citri subsp. citri quanto à patogenicidade." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/121954.

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Made available in DSpace on 2015-04-09T12:28:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-07-25Bitstream added on 2015-04-09T12:47:59Z : No. of bitstreams: 1 000817146.pdf: 479870 bytes, checksum: d3427b349dc719a504332ddf82064866 (MD5)<br>O estudo da interação planta-patógeno é de grande importância para o entendimento do cancro cítrico, justificando assim a busca por genes que estejam ligados a patogenicidade e virulência em Xanthomonas citri subsp. citri (Xac), agente causal dessa doença. Neste estudo, foi realizada mutagênese aleatória por inserção do transposon EZ-Tn5 in vit

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6

Terrazas, Peterson Menezes. "Estudo do potencial genotóxico da Gutiferona A em diferentes células de camundongos in vitro /." Botucatu, 2013. http://hdl.handle.net/11449/108542.

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Orientador: Edson Luis Maistro<br>Banca: Maria Aparecida Marin Morales<br>Banca: Cláudia Aparecida Rainho<br>Resumo: Garcinia achachairu (GAC) é uma planta de origem boliviana que vem sendo utilizada na medicina popular para o tratamento de distúrbios gástricos, reumatismo, inflamações e como cicatrizante. A caracterização fitoquímica do extrato desta planta revelou que, uma benzofenona, a Gutiferona A (GA), é um dos seus compostos majoritários, que segundo estudos recentes, apresenta importante atividade antioxidante e antimicrobiana. Considerando o interesse em se aprofundar as análises do p

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7

Silva, Ana Carolina Buzinari da. "Análise de uma biblioteca de mutantes de Xanthomonas citri subsp. citri quanto à patogenicidade /." Jaboticabal, 2014. http://hdl.handle.net/11449/121954.

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Orientador: Maria Inês Tiraboschi Ferro<br>Coorientador: Jesus Aparecido Ferro<br>Banca: Flavia Maria de Souza Carvalho<br>Banca: Fabrício José Jaciani<br>Resumo: O estudo da interação planta-patógeno é de grande importância para o entendimento do cancro cítrico, justificando assim a busca por genes que estejam ligados a patogenicidade e virulência em Xanthomonas citri subsp. citri (Xac), agente causal dessa doença. Neste estudo, foi realizada mutagênese aleatória por inserção do transposon EZ-Tn5 in vitro no genoma da Xac. Obteve-se 8000 mutantes onde 292 foram conduzidos em ensaio experiment

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8

Baeza, Centurión Pablo 1989. "Understanding alternative splicing using deep mutagenesis." Doctoral thesis, Universitat Pompeu Fabra, 2020. http://hdl.handle.net/10803/668749.

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Alternative pre-mRNA splicing is a regulated step in eukaryotic gene expression in which introns are removed from the transcript and exons are joined together to form a mature mRNA. To study the effects of mutations on alternative splicing, we built a mutant library containing all combinations of 12 mutations that accumulated during the emergence of an alternatively-spliced human exon: FAS exon 6. This allowed us to study the effects of individual nucleotide substitutions in thousands of different closely-related genetic contexts. We show that the effect of the same mutation on exon inclusion

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9

Brown, Jeremy Stuart. "Signature tagged-mutagenesis of aspergillus fumigatus." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322287.

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10

Seaman, Jonathan. "Signature-tagged mutagenesis in Rhizobium leguminosarum." Thesis, University of Reading, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.499374.

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Rhizobia are a diverse group of symbiotic alpha-proteobacterial diazotrophs which enter a relationship with specific leguminous plants, in which the plant supplies the bacteria with required compounds whilst the bacteria reduce atmospheric nitrogen into ammonia that the plant uses as a nitrogen source. Modification of rhizobial strains has produced mutants more effective at fixing nitrogen, which in turn results in an increase in biomass of host plants under laboratory conditions but these strains are frequently out competed by wild-type strains in field studies or lost in the intervening year

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11

Hart, Nicola Jayne. "Molecular outcomes of mutagenesis in wheat." Thesis, University of East Anglia, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.445180.

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12

Brown, R. B. "Ultraviolet light mutagenesis in Myxococcus xanthus." Thesis, University of East Anglia, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373103.

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13

Herbert, Mark A. "Signature tagged mutagenesis of Haemophilus influenzae." Thesis, Open University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340734.

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14

Timms, Andrew Robert. "Spontaneous mutagenesis in stressed Escherichia coli." Thesis, Open University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244410.

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15

Basiran, Mohd Nazir. "DNA insertion mutagenesis in higher plants." Thesis, University of Leicester, 1988. http://hdl.handle.net/2381/35436.

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16

Rúnarsdóttir, Saga. "Site-Directed Mutagenesis Studies of FucO." Thesis, Uppsala universitet, Institutionen för kemi - BMC, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-235136.

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17

Al-Khatib, Haifa Yousef. "Site Directed Mutagenesis of Dienelactone Hydrolase." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc277940/.

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The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.

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18

Chen, Wei 1965. "Site Directed Mutagenesis Of Dienelactone Hydrolase." Thesis, University of North Texas, 1992. https://digital.library.unt.edu/ark:/67531/metadc500900/.

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The role of individual amino acid residues of the enzyme dienelactone hydrolase was investigated. Using the polymerase chain reaction (PCR), a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHI to EcoRI clcD subclone in the plasmid pUC19. Site-specific mutants of dienelactone hydrolase were created using mismatched oligonucleotides to prime DNA synthesis. Specifically modified proteins from mutated clcD genes (Arg 81 to alanine, Tyr 85 to phenylalanine and Arg 206 to alanine), were encoded by the mutant clones. Enzyme assays showed that dienelactone hydrolase activity of

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19

Williams, Neal Kenneth. "Overexpression and mutagenesis of mammalian dihydroorotase." Thesis, The University of Sydney, 1993. https://hdl.handle.net/2123/26596.

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A central 2.1 kb region from a partial cDNA clone of the hamster CAD gene, pCAD142, has been sub-cloned and its nucleotide sequence determined. N-terminal and internal amino acid sequences obtained from the purified dihydroorotase domain isolated from elastase digests of trifunctional dihydroorotate synthetase have been aligned with the deduced amino acid sequence from the CAD cDNA. These results show conclusively that dihydroorotase is the central domain of trifunctional dihydroorotate synthetase. A C-terminal sequence of two amino acid residues from the native dihydroorotase domain was al

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20

Daggett, Kelly Anne. "Development and applications of codon scanning mutagenesis a novel mutagenesis method that facilitates in-frame codon mutations /." College Park, Md. : University of Maryland, 2009. http://hdl.handle.net/1903/9989.

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Thesis (Ph.D.) -- University of Maryland, College Park, 2009.<br>Thesis research directed by: Dept. of Chemistry and Biochemistry. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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21

Ozato, Kenjiro. "Mutagenesis, speciation, and genome analysis in medaka." Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University, 1994. http://hdl.handle.net/2237/13793.

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22

Dobrowsky, Adrian. "DNA polymerase-catalyzed expansion mutagenesis in vitro." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ40041.pdf.

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23

Fowler, kay. "Transposon mutagenesis of Strepromyces coelicolor A3(2)." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.247105.

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24

Maughan, William Neil. "Pancreatic deoxyribonuclease I : a study using mutagenesis." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324794.

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25

Fontaine, Michael Christopher. "Allel-replacement mutagenesis of group A streptococci." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285338.

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26

Rogers, S. D. "DNA repair and mutagenesis in Penicillium chrysogenum." Thesis, University of Westminster, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233044.

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27

Scott, Henry Hepburne. "#alpha#B-crystallin expression, mutagenesis and immunoreactivity." Thesis, University of Reading, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284449.

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28

Hall, Neil. "Mutagenesis of nitrate reductase in Aspergillus nidulans." Thesis, University of Liverpool, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364155.

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29

Leggate, Daniel Richard. "Characterisation and mutagenesis of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621894.

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30

Smedley, Damian Paul. "Mutagenesis of a Bacillus thuringiensis entomocidal toxin." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627459.

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31

Vermulst, Marc. "Untangling mitochondrial mutagenesis and aging in mice /." Thesis, Connect to this title online; UW restricted, 2008. http://hdl.handle.net/1773/6321.

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32

Chang, Thomas Kyu-Young Barton Jacqueline K. Gray Harry B. Richards John. "Gene synthesis, expression, and mutagenesis of azurin /." Diss., Pasadena, Calif. : California Institute of Technology, 1991. http://resolver.caltech.edu/CaltechETD:etd-06142007-132218.

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33

SICOURI, LARA. "TUMOR SUPPRESSOR MUTAGENESIS DRIVEN BY DNA DEAMINASE." Doctoral thesis, Università degli Studi di Milano, 2016. http://hdl.handle.net/2434/365729.

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Genomic instability is commonly associated with pathological disorders including cancer. The progressive accumulation of genetic abnormalities in cancer-associated genes can confer cellular autonomous proliferation, contributing to the oncogenic transformation. Although animal models have been instrumental to the understanding of the molecular mechanisms responsible for tumor progression, there are severe limitations for success. This thesis work aimed at the development of an innovative animal model that could recapitulate the ongoing lifelong accumulation of DNA lesions, leading to neoplasti

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34

Terrazas, Peterson Menezes [UNESP]. "Estudo do potencial genotóxico da Gutiferona A em diferentes células de camundongos in vitro." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/108542.

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Made available in DSpace on 2014-08-13T14:50:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-07-19Bitstream added on 2014-08-13T18:00:52Z : No. of bitstreams: 1 000753046.pdf: 1011046 bytes, checksum: 0e27bed9fbd866a00fb519db604313c6 (MD5)<br>Garcinia achachairu (GAC) é uma planta de origem boliviana que vem sendo utilizada na medicina popular para o tratamento de distúrbios gástricos, reumatismo, inflamações e como cicatrizante. A caracterização fitoquímica do extrato desta planta revelou que, uma benzofenona, a Gutiferona A (GA), é um dos seus compostos majoritários, que segundo

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35

Sheng, Mei. "Site Directed Mutagenesis of β-Ketoadipate Succinyl-Coenzyme A Transferase II from Acinetobacter Calcoaceticus". Thesis, University of North Texas, 1993. https://digital.library.unt.edu/ark:/67531/metadc279281/.

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The role of specific amino acid residues in β-ketoadipate succinyl-coenzyme A transferase II from Acinetobacter calcoaceticus was investigated. A 1412 base pair BamiHI-EcoRI fragment carrying the catIJ genes was amplified by polymerase chain reaction and inserted into pUCl9 to generate the plasmid pCATEl9. Escherichia coli DH5α (pCATEl9) carrying only the catlJ genes expressed 3-fold higher enzyme activity than the parent strain. Two mutants were constructed by site directed mutagenesis so that glutamate was replaced by a glutamine at positions Gln155 and Gln193 in the ß subunit of the primary

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36

Gong, Shanzhong. "Evaluation of targetron based mutagenesis in Ehrlichia chaffeensis." Thesis, Manhattan, Kan. : Kansas State University, 2010. http://hdl.handle.net/2097/4127.

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37

White, Malcolm F. "Yeast phosphoglycerate mutase studied by site-directed mutagenesis." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/24419.

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38

Wright, Tom. "Post-translational mutagenesis : radical methods for protein modification." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:774aa93a-489e-4051-aa01-b33781215968.

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The naturally occurring post-translational modification of proteins expands the chemical and structural diversity available for protein function. Enzymatic processes are known to modify proteins after translation, and in many cases these modifications have been demonstrated to be critical for biological function. However, the paucity of methods for introduction of site-specific modifications to proteins remains a key obstacle to their biochemical study. Access to these naturally modified proteins has long been complicated by their low abundance, difficulties in purifying homogeneous samples fr

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39

Lee, Jong-min. "Mutagenesis of the Yeast ALR1 Mg Transport Gene." Thesis, University of Auckland, 2006. http://hdl.handle.net/2292/5822.

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Magnesium is an essential element and the most abundant divalent cation in eukaryotic and prokaryotic cells, but its transport is not well understood. Mutagenesis was used to study the function of the ALR1 (aluminium resistance) gene, which encodes the major Mg2+ uptake system in Saccharomyces cerevisiae. Random PCR mutagenesis was undertaken of the C-terminal part of ALR1 that is homologous to the bacterial CorA magnesium transport family. The mutants with the most severe phenotype all had amino acid changes in a small region of Alr1 containing the putative transmembrane (TM) domains. Eightee

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40

Themis, Michael. "Retrovirus insertional mutagenesis : experiences at the hprt locus." Thesis, Brunel University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307483.

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41

Bryant, J. M. "Expression, mutagenesis and properties of bacteriophage K1E endosialidase." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597038.

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A full length endoE gene construct has been generated from the partial clones used to sequence the gene. Recombinant endoE has been expressed and purified as a fusion protein that is catalytically active and possesses the same kinetic characteristics as the bacteriophage enzyme. The mature, active endoE is 76 kDa in size and is probably produced by the post-translational cleavage of a 90 kDa primary translation product. Recombinant endoE has been purified to homogeneity, in milligram quantities, with the fusion protein partner removed for further studies of the enzyme. The role of the C-termin

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42

Morais, Francisco Silverio. "Mutagenesis of cytochrome b-559 in Chlamydomonas reinhardtii." Thesis, Imperial College London, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.484183.

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43

Moseley, Gregory William. "Investigation of tetraspanin structure and function by mutagenesis." Thesis, University of Sheffield, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391041.

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44

Woodgate, R. "A mechanism for UV mutagenesis in Escherichia coli." Thesis, University of Sussex, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375858.

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45

Warburton, Johnny. "Selection and mutagenesis in cultured African violet plantlets." Thesis, University of Edinburgh, 1986. http://hdl.handle.net/1842/11945.

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46

Basran, Amrik. "Refolding, mutagenesis and characterisation of secretory phospholipase A2." Thesis, University of Leicester, 1996. http://hdl.handle.net/2381/35251.

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Bovine pancreatic phospholipase A2 (bPLA2) is a 14 kDa (123 amino acids) enzyme with seven disulphide bonds. Its primary function in vivo is the hydrolysis of dietary phospholipids. A T7 RNA polymerase based expression system was used to overexpress a synthetic gene encoding bovine pancreatic pro-phospholipase A2 in E. coli. The expressed protein was directed to the periplasmic space via an outer membrane secretory signal (OmpT) where, after translocation the protein formed insoluble inclusion bodies. Translocation efficiency was significantly increased (from about 25% to over 90%) when protei

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47

Wilson, Mark Steven Michael. "Mutagenesis of nifE and nifN from Azotobacter vinelandii." Thesis, Virginia Tech, 1988. http://hdl.handle.net/10919/43071.

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The products of nifE and nifN from Azotobacter vinelandii, which are involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co) co) from nitrogenase, have been analyzed using a variety of mutagenic techniques. NifE was the object of several site-specific, amino acid substitutions that were designed to elicit information regarding metal cluster ligands, subunit-subunit interactions, and the proposed transfer of FeMo-co.from a nifEN-products complex to the apo-MoFe protein. A model of metal cluster binding; regions within the nifEN-products is discussed insofar as it relates t

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48

Romier, Christophe. "Crystallographic and mutagenesis studies of tRNA-guanine transglycosylase." Aix-Marseille 2, 1997. http://www.theses.fr/1997AIX22011.

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L'enzyme procaryote trna-guanine transglycosylase (tgt) catalyse l'echange, sur les arnt specifiques pour asn, asp, his et tyr, de la guanine a la position wobble avec la 7-aminomethyle-7-deazaguanine (preq1), un precurseur de la base hypermodifiee queuine. La tgt de zymomonas mobilis, une proteine de 43 kda, a ete purifiee et cristallisee pour son etude structurale par cristallographie aux rayons x. Sa structure a ete resolue par la methode de remplacement isomorphe multiple a l'aide de six derives aux atomes lourds independants et a ete affinee a une resolution de 1,85 angstroms. La tgt adop

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49

Shah, Nehal Rajendra. "Towards Novel Methods of Mutagenesis for Histophilus somni." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/43708.

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Histophilus somni is an etiologic agent of shipping fever pneumonia, myocarditis, and other systemic diseases in bovines, although nonpathogenic commensal strains also exist. Virulence factors that have been identified in H. somni include biofilm formation, lipooligosaccharide phase variation, immunoglobulin binding proteins, survival in phagocytic cells, and many others. To identify genes responsible for virulence, an efficient mutagenesis system is needed. Mutagenesis of H. somni using allelic exchange is difficult due to its tight restriction modification system. Mutagenesis by natural tra

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50

Wang, Xiaoshan. "Site-Directed Mutagenesis in Francisella Tularensis by Allelic." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/36440.

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<p> Francisella tularensis is a Gram-negative, facultative intracellular coccobacillus and the etiologic agent of tularemia for a wide variety of vertebrate and invertebrate animal species. Several species and subspecies of Francisella are currently recognized. However, the majority of infections are caused by F. tularensis subspecies tularensis (type A) and subspecies holarctica (type B). Given the low infectious dose, multiple transmission routes, severity of illness, and lack of licensed vaccines, F. tularensis has long been considered a potential biological weapon and is now classified as

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