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Dissertations / Theses on the topic 'Mutant library'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Thaipisuttikul, Lyarit. "Identification of genes required for anaerobic growth of Pseudomonas aeruginosa using a comprehensive transposon mutant library /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/10281.

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2

Kean, Iain. "Development of polymerase lithographic technology and analysis of an E. coli transposon mutant library for nitrite sensitivity." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/8169/.

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3

Betts, Melania Figueroa. "Identification of New Pathogenicity Genes in Magnaporthe Oryzae through the Construction of an Agrobacterium Tumefacines-Mediated Insertion Mutant Library." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/194453.

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An understanding of plant pathogen-host interactions is essential to design efficient strategies to control disease in crops. Magnaporthe oryzae, an ascomyceteous fungus and causal agent of rice blast disease, is a model organism to study host-microbe interactions. The overall aim of this dissertation project was to identify genes involved in pathogenicity through the construction and characterization of a random insertional mutagenesis library. In order to saturate the genome with DNA inserts, a collection of >54,000 insertion lines of the M. oryzae strain 70-15 was generated via two transfor

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4

Kurtovic, Sanela. "Directed Evolution of Glutathione Transferases Guided by Multivariate Data Analysis." Doctoral thesis, Uppsala University, Department of Biochemistry and Organic Chemistry, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8718.

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<p>Evolution of enzymes with novel functional properties has gained much attention in recent years. Naturally evolved enzymes are adapted to work in living cells under physiological conditions, circumstances that are not always available for industrial processes calling for novel and better catalysts. Furthermore, altering enzyme function also affords insight into how enzymes work and how natural evolution operates. </p><p>Previous investigations have explored catalytic properties in the directed evolution of mutant libraries with high sequence variation. Before this study was initiated, funct

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5

Li, Yanhui. "Construction and Analysis of a Genome-Wide Insertion Library in Schizosaccharomyces pombe Reveals Novel Aspects of DNA Repair." Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1413927620.

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6

Tan, Pauline H. "Sequence Specificity of Src Homology-2 Domains." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1324406526.

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7

Flor, Duro Alejandra. "Characterization of Genes and Functions Required by Multidrug-resistant Enterococci to Colonize the Intestine." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/166494.

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[ES] Las bacterias resistentes a múltiples antibióticos, como el Enterococo resistente a vancomicina (ERV), son un problema creciente en los pacientes hospitalizados, por lo que se necesita estrategias alternativas para combatir estos patógenos. Las infecciones causadas por ERV suelen comenzar con la colonización del tracto intestinal, un paso crucial que se afectado por la presencia de la microbiota. Sin embargo, los antibióticos alteran la microbiota y esto promueve la colonización de ERV. Una vez que el patógeno ha colonizado el intestino, alcanza niveles muy altos pudiendo diseminar a otro

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8

Hall, Qi. "Embryogenesis in Arabidopsis thaliana: Mutant library construction and embryo mutant identification and characterization." 2001. https://scholarworks.umass.edu/dissertations/AAI3012133.

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Embryogenesis plays a central role in the plant life cycle. It starts after fertilization. A single zygote divides asymmetrically, giving rise a small apical cell and a larger basal cell. The small apical cell undergoes precise cell divisions, passes through 2, 4 and 8 cell stages, followed by the protoderm, globular, heart, torpedo and cotyledon stage, in the process of forming a mature embryo. In embryo development a large number of genes are estimated to be involved and interact with each other during embryogenesis. We demonstrated that the RSH gene was required for normal embryo developmen

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9

Yang, Hui-Ching, and 楊惠晴. "Selection of biofilm formation related-genes from a Klebsiella pneumoniae mutant library." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/36052827283316395885.

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碩士<br>國立臺灣大學<br>微生物學研究所<br>95<br>Klebsiella pneumoniae is an opportunistic pathogen associated with nosocomial infections such as urinary tract infection, bacteremia and pneumonia. The biofilm formation of bacteria has been known to involve in the increasing resistance to antibiotic, antibacterial, and host immune responses. In order to explore the genes responsible for the biofilm formation in K. pneumoniae, a NTUH-K2044 mutant transposon library was screened by biofilm microtiter plate assay. Three mutants revealed decreased biofilm formation, and three mutants revealed increased biofilm for

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10

Sul, Soon-Hee. "Insights into the Serpin inhibitory mechanism from structures of Mutant Serpins in the Mechaelis Complex." 2008. http://www4.utsouthwestern.edu/library/ETD/etdDetails.cfm?etdID=382.

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11

Chen, Yi-Yin, and 陳依吟. "Mycobacterium marinum mmar_2318 and mmar_2319 are Responsible for Lipooligosaccharide Biosynthesis and Virulence towards Dictyostelium: screening in a transposon mutant library." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/15798083985838293381.

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博士<br>國立臺灣大學<br>微生物學研究所<br>104<br>Resistance to phagocyte killing is an important virulence factor in mycobacteria. Dictyostelium has been used to study the interaction between phagocytes and bacteria, given its similarity to the mammalian macrophage. Here, we investigated the genes responsible for virulence to Dictyostelium by screening 1728 transposon mutants of the Mycobacterium marinum NTUH-M6094 strain. A total of 30 mutants that were permissive for Dictyostelium growth were identified. These mutants revealed interruptions in 20 distinct loci. Of the 20 loci, six genes (losA, mmar_2318, m

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12

Syu, Zun-Jie, and 許尊傑. "Identification of rice genes associated with susceptibility or resistance to Pythium through the screening of Taiwan Rice Insertional Mutant library." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/45697639703421883766.

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碩士<br>國立中興大學<br>植物病理學系所<br>102<br>Chapter 1 Rice is one of the most important staple foods that feeds more than three billion people in the world. Rice diseases occurred during rice growing in the fields, such as seedling blight, blast, sheath blight, bacterial blight and bakana disease, and are a major obstacle for achieving optimal yields. Seedling blight is also known as water-mold disease, seed rot, and seedling damping-off. The disease is caused by several different pathogens and Pythium species is one of the major pathogens. Pythium infects roots of rice by zoospores or oospores spread i

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13

Grothe, Amberly Nicole. "Identifying Factors Controlling Cell Shape and Virulence Gene Expression in Borrelia Burgdorferi." Thesis, 2019. http://hdl.handle.net/1805/20529.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Lyme disease is a multi-system inflammatory disorder that is currently the fastest growing arthropod-borne disease in the United States. The Lyme disease pathogen, Borrelia burgdorferi, exists within an enzootic cycle consisting of Ixodes tick vectors and a variety of vertebrate hosts. Borrelia lies within a distinct clade of microorganisms known as spirochetes which exhibit a unique spiral morphology. The underlying genetic mechanisms controlling for borrelial morphologies are still being discovered. One flagellar protei

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14

Markus, Ralf [Verfasser]. "Complementation of the salt sensitive S. cerevisiae mutant cch1 with an A. thaliana cDNA library and analysis of isolated genes / vorgelegt von Ralf Markus." 2001. http://d-nb.info/963836145/34.

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15

Váňová, Jana. "Usměrněná evoluce myšího polyomaviru." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-343861.

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The method of directed evolution represents a new approach to generate proteins with new or altered properties. The principle of directed evolution is random mutagenesis of the coding sequence for a protein of our interest followed by selection of generated mutants for the desired property. The aim of this pilot study was to investigate the possibility of utilization of directed evolution for alteration of mouse polyomavirus original tropism and virus retargeting to a model prostate cancer cell line. To generate randomly mutated gene encoding the major capsid protein of mouse polyomavirus, whi

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16

Truong, Duy. "A library screen for yeast mutants defective in transformation by the lithium acetate/single stranded DNA/polyethylene glycol method." 2008. http://hdl.handle.net/1993/20937.

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