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Journal articles on the topic 'Mutant library'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Lokody, Isabel. "Human mutant-gene library." Nature Reviews Genetics 14, no. 10 (2013): 679. http://dx.doi.org/10.1038/nrg3593.

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Nilsson, Martin, Michael Givskov, Svante Twetman, and Tim Tolker-Nielsen. "Inactivation of the pgmA Gene in Streptococcus mutans Significantly Decreases Biofilm-Associated Antimicrobial Tolerance." Microorganisms 7, no. 9 (2019): 310. http://dx.doi.org/10.3390/microorganisms7090310.

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Screening of a Streptococcus mutans mutant library indicated that pgmA mutants displayed a reduced biofilm-associated tolerance toward gentamicin. The biofilms formed by the S. mutans pgmA mutant also displayed decreased tolerance towards linezolid and vancomycin compared to wild-type biofilms. On the contrary, the resistance of planktonic S. mutans pgmA cells to gentamycin, linezolid, and vancomycin was more similar to wild-type levels. Investigations of biofilms grown in microtiter trays and on submerged glass slides showed that pgmA mutants formed roughly the same amount of biofilm as the w

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Held, Kiara, Elizabeth Ramage, Michael Jacobs, Larry Gallagher, and Colin Manoil. "Sequence-Verified Two-Allele Transposon Mutant Library for Pseudomonas aeruginosa PAO1." Journal of Bacteriology 194, no. 23 (2012): 6387–89. http://dx.doi.org/10.1128/jb.01479-12.

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ABSTRACTMutant hunts using comprehensive sequence-defined libraries make it possible to identify virtually all of the nonessential functions required for different bacterial processes. However, the success of such screening depends on the accuracy of mutant identification in the mutant library used. To provide a high-quality library forPseudomonas aeruginosaPAO1, we created a sequence-verified collection of 9,437 transposon mutants that provides genome coverage and includes two mutants for most genes. Mutants were cherry-picked from a larger library, colony-purified, and resequenced both indiv

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Chiu, Ya-Fang, Chao-Ping Tung, Yu-Hisu Lee, et al. "A comprehensive library of mutations of Epstein–Barr virus." Journal of General Virology 88, no. 9 (2007): 2463–72. http://dx.doi.org/10.1099/vir.0.82881-0.

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A mutant library of 249 mutants with mutations that span the entire Epstein–Barr virus (EBV) genome was generated by transposition with EZ : : TN <KAN-2> and insertion with an apramycin resistance gene by a PCR-targeting method. This study also demonstrates the feasibility of generating deletions and site-specific mutations in the BRLF1 promoter on the EBV genome to determine the regions in the promoter that are crucial to transcription. Analysing BZLF1 and BRLF1 mutants by microarray analysis revealed that these two genes regulate the transcription of EBV lytic genes differently. A BZLF

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Tao, L., and J. M. Tanzer. "Novel Sucrose-dependent Adhesion Co-factors in Streptococcus mutans." Journal of Dental Research 81, no. 7 (2002): 505–10. http://dx.doi.org/10.1177/154405910208100715.

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Streptococcus mutans glucosyltransferases form extracellular glucans from sucrose to promote adhesion to the teeth. We tested whether additional factors are involved in S. mutans sucrose-dependent adhesion. By screening a pVA891-insertion mutant library of S. mutans LT11, we isolated four clones deficient in adhesion to glass in the presence of sucrose, but normal in glucosyltransferase activities. The genetic loci flanking the insertion sites were retrieved and identified. They encode glycerol-3-phosphate dehydrogenase, an ABC transporter, a multidrug-efflux pump, and either the ribulose mono

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Schaack, Jerome, William Y. Ho, Shawna Tolman, et al. "Construction and Preliminary Characterization of a Library of “Lethal” Preterminal Protein Mutant Adenoviruses." Journal of Virology 73, no. 11 (1999): 9599–603. http://dx.doi.org/10.1128/jvi.73.11.9599-9603.1999.

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ABSTRACT Adenoviruses containing lethal in-frame insertion mutant alleles of the preterminal protein (pTP) gene were constructed with cell lines that express pTP. Thirty in-frame insertion mutant alleles, including 26 alleles previously characterized as lethal and 4 newly constructed mutant alleles, were introduced into the viral chromosome in place of the wild-type pTP gene. The viruses were tested for ability to form plaques at 37°C in HeLa-pTP cells and at 32°C and 39.5°C in HeLa cells. Two of the newly constructed viruses exhibited temperature sensitivity for plaque formation, one virus di

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CHEN, Chen, Qing-zhi CUI, San-wen HUANG, et al. "An EMS mutant library for cucumber." Journal of Integrative Agriculture 17, no. 7 (2018): 1612–19. http://dx.doi.org/10.1016/s2095-3119(17)61765-9.

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Wu, Zhe, Zhenzhen Liu, Shuangfeng Chang, and Yuxuan Zhao. "An EMS mutant library for carrot and genetic analysis of some mutants." Breeding Science 70, no. 5 (2020): 540–46. http://dx.doi.org/10.1270/jsbbs.20020.

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Dong-Mei, Cao, Fan Xi-Ying, Wang Yun-Shan, and Kang Li-Fang. "Activation tagging library construction and mutant phenotype analysis." Chinese Journal of Agricultural Biotechnology 5, no. 3 (2008): 217–21. http://dx.doi.org/10.1017/s1479236208002325.

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AbstractActivation tagging plays an important role in plant genomics studies. In this paper, an activation tagging library containing 50 000 Basta-resistant lines was constructed by mediated transformation of the mutant bzr1-D of Aribidopsis thaliana. Among these transformants, 47 lines showed obvious phenotypes, including late flowering time, dwarf growth habit, changed leaf shape, longer leaf petiole, leaves lacking trichomes, sterility and no kink between stem/leaf. Some T-DNA flanking sequences were obtained by TAIL (thermal asymmetric interlaced) and nested PCR. Results showed that the mu

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Jacobs, M. A., A. Alwood, I. Thaipisuttikul, et al. "Comprehensive transposon mutant library of Pseudomonas aeruginosa." Proceedings of the National Academy of Sciences 100, no. 24 (2003): 14339–44. http://dx.doi.org/10.1073/pnas.2036282100.

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Abid, Muhammad Ali, Peilin Wang, Tao Zhu, et al. "Construction of Gossypium barbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement." International Journal of Molecular Sciences 21, no. 18 (2020): 6505. http://dx.doi.org/10.3390/ijms21186505.

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Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton. Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with

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Garaigorta, Urtzi, Ana M. Falcón, and Juan Ortín. "Genetic Analysis of Influenza Virus NS1 Gene: a Temperature-Sensitive Mutant Shows Defective Formation of Virus Particles." Journal of Virology 79, no. 24 (2005): 15246–57. http://dx.doi.org/10.1128/jvi.79.24.15246-15257.2005.

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ABSTRACT To perform a genetic analysis of the influenza A virus NS1 gene, a library of NS1 mutants was generated by PCR-mediated mutagenesis. A collection of mutant ribonucleic proteins containing the nonstructural genes was generated from the library that were rescued for an infectious virus mutant library by a novel RNP competition virus rescue procedure. Several temperature-sensitive (ts) mutant viruses were obtained by screening of the mutant library, and the sequences of their NS1 genes were determined. Most of the mutations identified led to amino acid exchanges and concentrated in the N

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Ha, Dae-Gon, Megan E. Richman, and George A. O'Toole. "Deletion Mutant Library for Investigation of Functional Outputs of Cyclic Diguanylate Metabolism in Pseudomonas aeruginosa PA14." Applied and Environmental Microbiology 80, no. 11 (2014): 3384–93. http://dx.doi.org/10.1128/aem.00299-14.

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ABSTRACTWe constructed a library of in-frame deletion mutants targeting each gene inPseudomonas aeruginosaPA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library.

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Song, Jae Kwang, and Joon Shick Rhee. "Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular Engineering." Applied and Environmental Microbiology 66, no. 3 (2000): 890–94. http://dx.doi.org/10.1128/aem.66.3.890-894.2000.

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ABSTRACT The thermal stability and catalytic activity of phospholipase A1 from Serratia sp. strain MK1 were improved by evolutionary molecular engineering. Two thermostable mutants were isolated after sequential rounds of error-prone PCR performed to introduce random mutations and filter-based screening of the resultant mutant library; we determined that these mutants had six (mutant TA3) and seven (mutant TA13) amino acid substitutions. Different types of substitutions were found in the two mutants, and these substitutions resulted in an increase in nonploar residues (mutant TA3) or in differ

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Rockwell, Nathan, Max Staller, Maria Cannella, Barak Cohen, and Joshua Rubin. "GENE-59. NOT ALL p53 MUTATIONS ARE CREATED EQUAL: A MURINE ASTROCYTE MODEL FOR HIGH-THROUGHPUT FUNCTIONAL ASSESSMENT OF p53 MISSENSE MUTATIONS." Neuro-Oncology 21, Supplement_6 (2019): vi110. http://dx.doi.org/10.1093/neuonc/noz175.461.

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Abstract The tumor suppressor TP53 (p53) is the most commonly mutated gene in cancer and among the most frequently mutated genes in glioblastoma (GBM). The majority of p53 mutations in GBM are missense mutations in the DNA binding domain that lead to the production of full length mutant p53 protein. In addition to the complete loss of tumor suppressor function, these mutations have gain-of-function (GOF) properties either through attenuation of wild-type function or neomorphic functions. The variability in GOF mutations results in heterogeneity in cancer phenotypes between mutants that remain

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Chen, Qiang, Hui Wu, and Paula M. Fives-Taylor. "Construction of a Novel Transposon Mutagenesis System Useful in the Isolation of Streptococcus parasanguis Mutants Defective in Fap1 Glycosylation." Infection and Immunity 70, no. 12 (2002): 6534–40. http://dx.doi.org/10.1128/iai.70.12.6534-6540.2002.

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ABSTRACT Streptococcus parasanguis, a primary colonizer of the tooth surface, has long, peritrichous fimbriae. A fimbria-associated protein, Fap1, is identified as an adhesin of S. parasanguis FW213. The mature Fap1 protein is glycosylated, and the glycosylation is required for fimbria biogenesis and bacterial adhesion. Little is known about the mechanism of Fap1 glycosylation due to the lack of identifiable mutants. A novel transposon mutagenesis system was established and used to generate a mutant library. Screening of the library with a monoclonal antibody specific for a glycan epitope of F

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Breidenstein, Elena B. M., Bhavjinder K. Khaira, Irith Wiegand, Joerg Overhage, and Robert E. W. Hancock. "Complex Ciprofloxacin Resistome Revealed by Screening a Pseudomonas aeruginosa Mutant Library for Altered Susceptibility." Antimicrobial Agents and Chemotherapy 52, no. 12 (2008): 4486–91. http://dx.doi.org/10.1128/aac.00222-08.

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ABSTRACT Pseudomonas aeruginosa offers substantial therapeutic challenges due to its high intrinsic resistance to many antibiotics and its propensity to develop mutational and/or adaptive resistance. The PA14 comprehensive mutant library was screened for mutants exhibiting either two- to eightfold increased susceptibilities (revealing genes involved in intrinsic resistance) or decreased susceptibilities (mutational resistance) to the fluoroquinolone ciprofloxacin. Thirty-five and 79 mutants with increased and decreased susceptibilities, respectively, were identified, as confirmed by broth dilu

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Sabbagh, Yves, Esther Thériault, François Sanschagrin, Normand Voyer, Timothy Palzkill та Roger C. Levesque. "Characterization of a PSE-4 Mutant with Different Properties in Relation to Penicillanic Acid Sulfones: Importance of Residues 216 to 218 in Class A β-Lactamases". Antimicrobial Agents and Chemotherapy 42, № 9 (1998): 2319–25. http://dx.doi.org/10.1128/aac.42.9.2319.

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ABSTRACT Class A β-lactamases are inactivated by the suicide inactivators sulbactam, clavulanic acid, and tazobactam. An examination of multiple alignments indicated that amino acids 216 to 218 differed among class A enzymes. By random replacement mutagenesis of codons 216 to 218 in PSE-4, a complete library consisting of 40,864 mutants was created. The library of mutants with mutations at positions 216 to 218 in PSE-4 was screened on carbenicillin and ampicillin with the inactivator sulbactam; a collection of 14 mutants was selected, and their bla genes were completely sequenced. Purified wil

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Wang, Lei, Jie Zheng, Yanzhong Luo, et al. "Construction of a genomewide RNAi mutant library in rice." Plant Biotechnology Journal 11, no. 8 (2013): 997–1005. http://dx.doi.org/10.1111/pbi.12093.

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Xie, Yong-Fang, Bo-Chu Wang, Biao Li, et al. "Construction of cDNA library of cotton mutant (Xiangmian-18) library during gland forming stage." Colloids and Surfaces B: Biointerfaces 60, no. 2 (2007): 258–63. http://dx.doi.org/10.1016/j.colsurfb.2007.06.020.

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Nhat, Vuong Quoc, Yusuke Kazama, Kotaro Ishii, et al. "Double Mutant Analysis with the Large Flower Mutant, ohbana1, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in Arabidopsis thaliana." Plants 10, no. 9 (2021): 1881. http://dx.doi.org/10.3390/plants10091881.

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Two growth processes, cell proliferation and expansion, determine plant species-specific organ sizes. A large flower mutant in Arabidopsis thaliana, ohbana1 (ohb1), was isolated from a mutant library. In the ohb1 flowers, post-mitotic cell expansion and endoreduplication of nuclear DNA were promoted. The whole-genome resequencing and genetic analysis results showed that the loss of function in MEDIATOR16 (MED16), a mediator complex subunit, was responsible for the large flower phenotypes exhibited by ohb1. A phenotypic analysis of the mutant alleles in MED16 and the double mutants created by c

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Hurst, Sheldon, Holli Rowedder, Brandye Michaels, et al. "Elucidation of the Photorhabdus temperata Genome and Generation of a Transposon Mutant Library To Identify Motility Mutants Altered in Pathogenesis." Journal of Bacteriology 197, no. 13 (2015): 2201–16. http://dx.doi.org/10.1128/jb.00197-15.

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ABSTRACTThe entomopathogenic nematodeHeterorhabditis bacteriophoraforms a specific mutualistic association with its bacterial partnerPhotorhabdus temperata. The microbial symbiont is required for nematode growth and development, and symbiont recognition is strain specific. The aim of this study was to sequence the genome ofP. temperataand identify genes that plays a role in the pathogenesis of thePhotorhabdus-Heterorhabditissymbiosis. A draft genome sequence ofP. temperatastrain NC19 was generated. The 5.2-Mb genome was organized into 17 scaffolds and contained 4,808 coding sequences (CDS). A

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Newton, Irene L. G., and Kathy B. Sheehan. "Passage of Wolbachia pipientis through Mutant Drosophila melanogaster Induces Phenotypic and Genomic Changes." Applied and Environmental Microbiology 81, no. 3 (2014): 1032–37. http://dx.doi.org/10.1128/aem.02987-14.

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ABSTRACTWolbachia pipientisis a nearly ubiquitous, maternally transmitted bacterium that infects the germ line of insect hosts. Estimates are thatWolbachiainfects 40 to 60% of insect species on the planet, making it one of the most prevalent infections on Earth. However, we know surprisingly little about the molecular mechanisms used byWolbachiato infect its hosts. We passagedWolbachiathrough normally restrictiveDrosophila melanogasterhosts, bottleneckingWolbachiathrough stochastic segregation while simultaneously selecting for mutants that could recolonize these previously restrictive hosts.

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Li, Weixing, Zhichong He, Shunbo Yang, Yunling Ye, Huiru Jiang, and Li Wang. "Construction and analysis of a library of miRNA in gold-coloured mutant leaves of Ginkgo biloba L." Folia Horticulturae 31, no. 1 (2019): 81–92. http://dx.doi.org/10.2478/fhort-2019-0005.

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AbstractTo gain insights into the regulatory networks of miRNAs related to golden colour formation in Ginkgo biloba leaves, we constructed an sRNA library of golden-green striped mutant leaves. A total of 213 known miRNAs comprising 54 miRNA families were obtained, and 214 novel miRNAs were identified in the mutant leaves. We further constructed a normal green leaf sRNA library as a control and compared the expression of miRNAs between mutant and normal leaves. We found 42 known and 54 novel differential expression candidate miRNAs; 39 were up-regulated and 57 down-regulated in mutants compare

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Wakao, Setsuko, Patrick M. Shih, Katharine Guan, et al. "Discovery of photosynthesis genes through whole-genome sequencing of acetate-requiring mutants of Chlamydomonas reinhardtii." PLOS Genetics 17, no. 9 (2021): e1009725. http://dx.doi.org/10.1371/journal.pgen.1009725.

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Large-scale mutant libraries have been indispensable for genetic studies, and the development of next-generation genome sequencing technologies has greatly advanced efforts to analyze mutants. In this work, we sequenced the genomes of 660 Chlamydomonas reinhardtii acetate-requiring mutants, part of a larger photosynthesis mutant collection previously generated by insertional mutagenesis with a linearized plasmid. We identified 554 insertion events from 509 mutants by mapping the plasmid insertion sites through paired-end sequences, in which one end aligned to the plasmid and the other to a chr

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Nissinen, Riitta, Yunjian Xia, Laura Mattinen, et al. "The Putative Secreted Serine Protease Chp-7 Is Required for Full Virulence and Induction of a Nonhost Hypersensitive Response by Clavibacter michiganensis subsp. sepedonicus." Molecular Plant-Microbe Interactions® 22, no. 7 (2009): 809–19. http://dx.doi.org/10.1094/mpmi-22-7-0809.

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Molecular biological studies on Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato, have gained greater feasibility due to the recent availability of whole genomic sequences and genetic tools for related taxa. Here, we describe the first report of construction and characterization of a transposon (Tn) mutant library of C. michiganensis subsp. sepedonicus sp. strain R10. Since virulence of R10 in potato has been shown previously to be associated with elicitation of a nonhost hypersensitive response (HR), the mutant library was screened initially for l

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Xu, Junqi, Yu Chen, Xi Mou, et al. "Mycobacterium smegmatis msmeg_3314 is involved in pyrazinamide and fluoroquinolones susceptibility via NAD+/NADH dysregulation." Future Microbiology 15, no. 6 (2020): 413–26. http://dx.doi.org/10.2217/fmb-2019-0071.

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Aim: To identify and characterize new mycobacterium pyrazinamide (PZA) resistance genes in addition to pncA, rpsA and panD. Materials & methods: To screen a Tn7 M. smegmatis mc2155 transposon library using 50 μM PZA and a PZA hypersensitive mutant (M492) was obtained. MIC was further used to confirm the hypersensitivity of M492 mutant by culturing the mutant in Middlebrook 7H9 liquid medium at 37°C. Results: msmeg_3314 is the gene underlying the hypersensitive phenotype of mutant M492. The observed resistance to PZA and fluoroquinolones involved the alteration of Mycobacterium cell wall pe

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Yu, Jie, Yaqi Wang, Dongmei Han, et al. "Identification of Streptococcus mutans genes involved in fluoride resistance by screening of a transposon mutant library." Molecular Oral Microbiology 35, no. 6 (2020): 260–70. http://dx.doi.org/10.1111/omi.12316.

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Gallagher, Larry A., Elizabeth Ramage, Michael A. Jacobs, Rajinder Kaul, Mitchell Brittnacher, and Colin Manoil. "A comprehensive transposon mutant library ofFrancisella novicida, a bioweapon surrogate." Proceedings of the National Academy of Sciences 104, no. 3 (2007): 1009–14. http://dx.doi.org/10.1073/pnas.0606713104.

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Alvarez-Ortega, Carolina, Irith Wiegand, Jorge Olivares, Robert E. W. Hancock та José Luis Martínez. "Genetic Determinants Involved in the Susceptibility of Pseudomonas aeruginosa to β-Lactam Antibiotics". Antimicrobial Agents and Chemotherapy 54, № 10 (2010): 4159–67. http://dx.doi.org/10.1128/aac.00257-10.

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ABSTRACT The resistome of P. aeruginosa for three β-lactam antibiotics, namely, ceftazidime, imipenem, and meropenem, was deciphered by screening a comprehensive PA14 mutant library for mutants with increased or reduced susceptibility to these antimicrobials. Confirmation of the phenotypes of all selected mutants was performed by Etest. Of the total of 78 confirmed mutants, 41 demonstrated a reduced susceptibility phenotype and 37 a supersusceptibility (i.e., altered intrinsic resistance) phenotype, with 6 mutants demonstrating a mixed phenotype, depending on the antibiotic. Only three mutants

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Malecki, Michal, and Jürg Bähler. "Identifying genes required for respiratory growth of fission yeast." Wellcome Open Research 1 (November 15, 2016): 12. http://dx.doi.org/10.12688/wellcomeopenres.9992.1.

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We have used both auxotroph and prototroph versions of the latest deletion-mutant library to identify genes required for respiratory growth on solid glycerol medium in fission yeast. This data set complements and enhances our recent study on functional and regulatory aspects of energy metabolism by providing additional proteins that are involved in respiration. Most proteins identified in this mutant screen have not been implicated in respiration in budding yeast. We also provide a protocol to generate a prototrophic mutant library, and data on technical and biological reproducibility of colon

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Shin, Sung Jae, Chia-wei Wu, Howard Steinberg, and Adel M. Talaat. "Identification of Novel Virulence Determinants in Mycobacterium paratuberculosis by Screening a Library of Insertional Mutants." Infection and Immunity 74, no. 7 (2006): 3825–33. http://dx.doi.org/10.1128/iai.01742-05.

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ABSTRACT Johne's disease, caused by Mycobacterium paratuberculosis infection, is a worldwide problem for the dairy industry and has a possible involvement in Crohn's disease in humans. To identify virulence determinants of this economically important pathogen, a library of 5,060 transposon mutants was constructed using Tn5367 insertion mutagenesis, followed by large-scale sequencing to identify disrupted genes. In this report, 1,150 mutants were analyzed and 970 unique insertion sites were identified. Sequence analysis of the disrupted genes indicated that the insertion of Tn5367 was more prev

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Yeung, Amy T. Y., Ellen C. W. Torfs, Farzad Jamshidi, et al. "Swarming of Pseudomonas aeruginosa Is Controlled by a Broad Spectrum of Transcriptional Regulators, Including MetR." Journal of Bacteriology 191, no. 18 (2009): 5592–602. http://dx.doi.org/10.1128/jb.00157-09.

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ABSTRACT Pseudomonas aeruginosa exhibits swarming motility on semisolid surfaces (0.5 to 0.7% agar). Swarming is a more than just a form of locomotion and represents a complex adaptation resulting in changes in virulence gene expression and antibiotic resistance. In this study, we used a comprehensive P. aeruginosa PA14 transposon mutant library to investigate how the complex swarming adaptation process is regulated. A total of 233 P. aeruginosa PA14 transposon mutants were verified to have alterations in swarming motility. The swarming-associated genes functioned not only in flagellar or type

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Cheng, Catherine, Sharon M. Tennant, Kristy I. Azzopardi, et al. "Contribution of the pst-phoU Operon to Cell Adherence by Atypical Enteropathogenic Escherichia coli and Virulence of Citrobacter rodentium." Infection and Immunity 77, no. 5 (2009): 1936–44. http://dx.doi.org/10.1128/iai.01246-08.

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ABSTRACT Strains of enteropathogenic Escherichia coli (EPEC) generally employ the adhesins bundle-forming pili (Bfp) and intimin to colonize the intestine. Atypical EPEC strains possess intimin but are negative for Bfp and, yet, are able to cause disease. To identify alternative adhesins to Bfp in atypical EPEC, we constructed a transposon mutant library of atypical EPEC strain E128012 (serotype O114:H2) using TnphoA. Six mutants that had lost the ability to adhere to HEp-2 cells were identified, and in all six mutants TnphoA had inserted into the pstSCAB-phoU (Pst) operon. To determine if the

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Tsujimoto, Yoshiyuki, Shingo Izawa, and Yoshiharu Inoue. "Cooperative Regulation of DOG2, Encoding 2-Deoxyglucose-6-Phosphate Phosphatase, by Snf1 Kinase and the High-Osmolarity Glycerol–Mitogen-Activated Protein Kinase Cascade in Stress Responses of Saccharomyces cerevisiae." Journal of Bacteriology 182, no. 18 (2000): 5121–26. http://dx.doi.org/10.1128/jb.182.18.5121-5126.2000.

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ABSTRACT We screened the genome of Saccharomyces cerevisiae for the genes responsive to oxidative stress by using the lacZtransposon-insertion library. As a result, we found that expression of the DOG2 gene coding for 2-deoxyglucose-6-phosphate phosphatase was induced by oxidative stress. The expression ofDOG2 was also induced by osmotic stress. We found a putative cis element (STRE, a stress response element) in the DOG2 promoter adjacent to a consensus sequence to which the Mig1p repressor is known to bind. The basal levels ofDOG2 gene expression were increased in amig1Δ mutant, while the de

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Overhage, Joerg, Shawn Lewenza, Alexandra K. Marr, and Robert E. W. Hancock. "Identification of Genes Involved in Swarming Motility Using a Pseudomonas aeruginosa PAO1 Mini-Tn5-lux Mutant Library." Journal of Bacteriology 189, no. 5 (2006): 2164–69. http://dx.doi.org/10.1128/jb.01623-06.

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ABSTRACT During a screening of a mini-Tn5-luxCDABE transposon mutant library of Pseudomonas aeruginosa PAO1 for alterations in swarming motility, 36 mutants were identified with Tn5 insertions in genes for the synthesis or function of flagellin and type IV pilus, in genes for the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. These mutants were differentially affected in swimming and twitching motility but in most cases had only a minor additional motility defect. Our data provide evidence that swarming is a more

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Kong, Lingfei, Zeyu Li, Qin Song, Xiaohong Li, and Keming Luo. "Construction of a Full-Length cDNA Over-Expressing Library to Identify Valuable Genes from Populus tomentosa." International Journal of Molecular Sciences 22, no. 7 (2021): 3448. http://dx.doi.org/10.3390/ijms22073448.

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Poplar wood is the main source of renewable biomass energy worldwide, and is also considered to be a model system for studying woody plants. The Full-length cDNA Over-eXpressing (FOX) gene hunting system is an effective method for generating gain-of-function mutants. Large numbers of novel genes have successfully been identified from many herbaceous plants according to the phenotype of gain-of-function mutants under normal or abiotic stress conditions using this system. However, the system has not been used for functional gene identification with high-throughput mutant screening in woody plant

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Balhadère, Pascale V., Andrew J. Foster, and Nicholas J. Talbot. "Identification of Pathogenicity Mutants of the Rice Blast Fungus Magnaporthe grisea by Insertional Mutagenesis." Molecular Plant-Microbe Interactions® 12, no. 2 (1999): 129–42. http://dx.doi.org/10.1094/mpmi.1999.12.2.129.

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Restriction enzyme-mediated DNA integration (REMI) mutagenesis was used to identify mutants of Magnaporthe grisea impaired in pathogenicity. Three REMI protocols were evaluated and the frequency of REMIs determined. An REMI library of 3,527 M. grisea transformants was generated in three genetic backgrounds, and 1,150 transformants were screened for defects in pathogenicity with a barley cut leaf assay. Five mutants were identified and characterized. Two mutants (2029 and 2050) were impaired in appressorium function. Two other mutants, 125 and 130, were altered in conidial morphology, conidioge

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Wiegand, Irith, Alexandra K. Marr, Elena B. M. Breidenstein, Kristen N. Schurek, Patrick Taylor, and Robert E. W. Hancock. "Mutator Genes Giving Rise to Decreased Antibiotic Susceptibility in Pseudomonas aeruginosa." Antimicrobial Agents and Chemotherapy 52, no. 10 (2008): 3810–13. http://dx.doi.org/10.1128/aac.00233-08.

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ABSTRACT Screening of the PA14 genomic transposon mutant library for resistance to ceftazidime, tobramycin, and ciprofloxacin led to the discovery of several mutants that appeared in more than one screen. Testing of the frequency of mutation to ciprofloxacin resistance revealed previously known mutator genes, including mutS and mutL, as well as mutators that have not yet been described for P. aeruginosa, including PA3958 and RadA (PA4609).

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Haynes, W. John, Brian Vaillant, Robin R. Preston, Yoshiro Saimi, and Ching Kung. "The Cloning by Complementation of the pawn-A Gene in Paramecium." Genetics 149, no. 2 (1998): 947–57. http://dx.doi.org/10.1093/genetics/149.2.947.

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Abstract The genetic dissection of a simple avoidance reaction behavior in Paramecium tetraurelia has shown that ion channels are a critical molecular element in signal transduction. Pawn mutants, for example, were originally selected for their inability to swim backward, a trait that has since been shown to result from the loss of a voltage-dependent calcium current. The several genes defined by this phenotype were anticipated to be difficult to clone since the 800-ploid somatic macronucleus of P. tetraurelia is a formidable obstacle to cloning by complementation. Nonetheless, when the macron

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Wei, Wei, Wenjun Zhu, Jiasen Cheng, et al. "CmPEX6, a Gene Involved in Peroxisome Biogenesis, Is Essential for Parasitism and Conidiation by the Sclerotial Parasite Coniothyrium minitans." Applied and Environmental Microbiology 79, no. 12 (2013): 3658–66. http://dx.doi.org/10.1128/aem.00375-13.

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ABSTRACTConiothyrium minitansis a sclerotial parasite of the plant-pathogenic fungusSclerotinia sclerotiorum, and conidial production and parasitism are two important aspects for commercialization of this biological control agent. To understand the mechanism of conidiation and parasitism at the molecular level, we constructed a transfer DNA (tDNA) insertional library with the wild-type strain ZS-1. A conidiation-deficient mutant, ZS-1TN22803, was uncovered through screening of this library. This mutant could produce pycnidia on potato dextrose agar (PDA), but most were immature and did not bea

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Kim, Sanggu, Yun-Cheol Kim, Hangfei Qi, Kunkai Su, Sherie L. Morrison, and Samson A. Chow. "Efficient Identification of Human Immunodeficiency Virus Type 1 Mutants Resistant to a Protease Inhibitor by Using a Random Mutant Library." Antimicrobial Agents and Chemotherapy 55, no. 11 (2011): 5090–98. http://dx.doi.org/10.1128/aac.00687-11.

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ABSTRACTEmergence of drug-resistant mutant viruses during the course of antiretroviral therapy is a major hurdle that limits the success of chemotherapeutic treatment to suppress human immunodeficiency virus type 1 (HIV-1) replication and AIDS progression. Development of new drugs and careful patient management based on resistance genotyping data are important for enhancing therapeutic efficacy. However, identifying changes leading to drug resistance can take years of clinical studies, and conventionalin vitroassays are limited in generating reliable drug resistance data. Here we present an ef

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Salama, Nina R., Benjamin Shepherd, and Stanley Falkow. "Global Transposon Mutagenesis and Essential Gene Analysis of Helicobacter pylori." Journal of Bacteriology 186, no. 23 (2004): 7926–35. http://dx.doi.org/10.1128/jb.186.23.7926-7935.2004.

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ABSTRACT We have constructed a genome-saturating mutant library of the human gastric pathogen Helicobacter pylori. Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363 transposon mutants in our library. While we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes. Comparison to the essential gene set of other bacteria revealed a surprisingly limited overlap with all organisms tested (11%), while 55% were essential in some org

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Minsavage, G. V., M. B. Mudgett, R. E. Stall, and J. B. Jones. "Importance of opgHXcv of Xanthomonas campestris pv. vesicatoria in Host-Parasite Interactions." Molecular Plant-Microbe Interactions® 17, no. 2 (2004): 152–61. http://dx.doi.org/10.1094/mpmi.2004.17.2.152.

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Tn5 insertion mutants of Xanthomonas campestris pv. vesicatoria were inoculated into tomato and screened for reduced virulence. One mutant exhibited reduced aggressiveness and attenuated growth in planta. Southern blot analyses indicated that the mutant carried a single Tn5 insertion not associated with previously cloned pathogenicity-related genes of X. campestris pv. vesicatoria. The wild-type phenotype of this mutant was restored by one recombinant plasmid (pOPG361) selected from a genomic library of X. campestris pv. vesicatoria 91–118. Tn3-gus insertion mutagenesis and sequence analyses o

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Kaup, Olaf, Ines Gräfen, Eva-Maria Zellermann, Rudolf Eichenlaub, and Karl-Heinz Gartemann. "Identification of a Tomatinase in the Tomato-Pathogenic Actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382." Molecular Plant-Microbe Interactions® 18, no. 10 (2005): 1090–98. http://dx.doi.org/10.1094/mpmi-18-1090.

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The insertion site of a transposon mutant of Clavibacter michiganensis subsp. michiganensis NCPPB382 was cloned and found to be located in the gene tomA encoding a member of the glycosyl hydrolase family 10. The intact gene was obtained from a cosmid library of C. michiganensis subsp. michiganensis. The deduced protein TomA (543 amino acids, 58 kDa) contains a predicted signal peptide and two domains, the N-terminal catalytic domain and a C-terminal fibronectin III-like domain. The closest well-characterized relatives of TomA were tomatinases from fungi involved in the detoxification of the to

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Bleumink-Pluym, Nancy M. C., Froukje Verschoor, Wim Gaastra, Bernard A. M. van der Zeijst, and Ben N. Fry. "A novel approach for the construction of a Campylobacter mutant library." Microbiology 145, no. 8 (1999): 2145–51. http://dx.doi.org/10.1099/13500872-145-8-2145.

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Wang, Tian-Wen, Hu Zhu, Xing-Yuan Ma, Ting Zhang, Yu-Shu Ma, and Dong-Zhi Wei. "Mutant Library Construction in Directed Molecular Evolution: Casting a Wider Net." Molecular Biotechnology 34, no. 1 (2006): 55–68. http://dx.doi.org/10.1385/mb:34:1:55.

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Purton, Saul, and Jean-David Rochaix. "Complementation of a Chlamydomonas reinhardtii mutant using a genomic cosmid library." Plant Molecular Biology 24, no. 3 (1994): 533–37. http://dx.doi.org/10.1007/bf00024121.

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Jiang, Qingwen, Weimin Zhang, Chenghao Liu, Yicong Lin, Qingyu Wu, and Junbiao Dai. "Dissecting PCNA function with a systematically designed mutant library in yeast." Journal of Genetics and Genomics 46, no. 6 (2019): 301–13. http://dx.doi.org/10.1016/j.jgg.2019.03.014.

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Wipf, Daniel, Mariam Benjdia, Enno Rikirsch, Sabine Zimmermann, Mechthild Tegeder, and Wolf B. Frommer. "An expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum." Genome 46, no. 2 (2003): 177–81. http://dx.doi.org/10.1139/g02-121.

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An oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of ~500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with unknown function, and the remaining 20% showed no significant similarity to any known genes. The ESTs had a GC content between 44 and 56%, with most of them having a GC content of 5254%, which could be correlated with GC contents of fungal genes. The library was successfully used to identify the Heb

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