Academic literature on the topic 'Mycelia reduction'
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Journal articles on the topic "Mycelia reduction"
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Supakitthanakorn, Salit, On-Uma Ruangwong, Choncharoen Sawangrat, Wimada Srisuwan, and Dheerawan Boonyawan. "Potential of Nonthermal Atmospheric-Pressure Dielectric Barrier Discharge Plasma for Inhibition of Athelia rolfsii Causing Southern Blight Disease in Lettuce." Agriculture 13, no. 1 (2023): 167. http://dx.doi.org/10.3390/agriculture13010167.
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Athelia rolfsii is one of the most destructive and aggressive fungal pathogens worldwide and causes southern blight disease of lettuce. A nonthermal atmospheric-pressure dielectric barrier discharge (DBD) plasma has attracted interest as an alternative control method to chemical usage because of its antimicrobial activity. Exposure of A. rolfsii to DBD plasma for 5, 10, 15, and 20 min resulted in in vitro fungal inhibition of mycelial discs and sclerotia. The results showed that DBD plasma exposure for 10 min completely inhibited fungal growth of mycelial discs, whereas exposure for over 20 min was required to inhibit the hyphal growth of sclerotia. Scanning electron microscopy (SEM) observations of mycelia and sclerotia abnormalities revealed laceration and damage of both mycelia and sclerotia. In addition, disease incidence and severity were reduced in mycelial and sclerotia inoculation following DBD plasma exposure for 15 and 20 min, respectively, compared with the positive control. In conclusion, the DBD plasma demonstrates antifungal activity against A. rolfsii via inhibition of fungal growth and reduction in disease incidence and severity. Therefore, DBD plasma has the potential to be applied in controlling southern blight disease of lettuce.
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Qu, Chenling, Zhuozhen Li, and Xiupin Wang. "UHPLC-HRMS-Based Untargeted Lipidomics Reveal Mechanism of Antifungal Activity of Carvacrol against Aspergillus flavus." Foods 11, no. 1 (2021): 93. http://dx.doi.org/10.3390/foods11010093.
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Aspergillus flavus is a common contaminant in grain, oil and their products. Its metabolite aflatoxin B1 (AFB1) has been proved to be highly carcinogenic. Therefore, it is of great importance to find possible antifungal substances to inhibit the growth and toxin production of Aspergillus flavus. Carvacrol (CV) was reported as a potent antifungal monoterpene derived from plants. In this paper, the antifungal effects and mechanism of CV on Aspergillus flavus were investigated. CV was shown good inhibition on the growth of Aspergillus flavus and the production of AFB1. CV used in concentrations ranging from 0, 50, 100 and 200 μg/mL inhibited the germination of spores, mycelia growth and AFB1 production dose-dependently. To explore the antifungal mechanism of CV on Aspergillus flavus, we also detected the ergosterol content of Aspergillus flavus mycelia, employed Scanning Electron Microscopy (SEM) to observe mycelia morphology and utilized Ultra-High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UHPLC-HRMS) to explore the lipidome profiles of Aspergillus flavus. The results showed that the production of ergosterol of mycelia was reduced as the CV treatment concentration increased. SEM photographs demonstrated a rough surface and a reduction in the thickness of hyphae in Aspergillus flavus treated with CV (200 µg/mL). In positive ion mode, 21 lipids of Aspergillus flavus mycelium were downregulated, and 11 lipids were upregulated after treatment with 200-µg/mL CV. In negative ion mode, nine lipids of Aspergillus flavus mycelium were downregulated, and seven lipids upregulated after treatment with 200-µg/mL CV. In addition, the analysis of different lipid metabolic pathways between the control and 200-µg/mL CV-treated groups demonstrated that glycerophospholipid metabolism was the most enriched pathway related to CV treatment.
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Alaba, Olaitan Adeji, and Olufunmilayo Aduramigba-Modupe Adefoyeke. "Botanical alternatives in management of fungal pathogens of seedling blight of cashew (Anacardium occidentale L.)." GSC Biological and Pharmaceutical Sciences 14, no. 1 (2021): 193–98. https://doi.org/10.5281/zenodo.4527752.
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Introduction: Cashew <em>(Anacardium occidentale L.)</em> is an important tree crop and seedling survival is pertinent to successful establishment. Cashew seedling is infected by blight pathogens causing more than 60% seedling lost, however pesticides residues related issues and high cost of chemical necessitate efficacy trials of aqueous extracts of <em>Mangifera indica</em>, <em>Azadirachta indica</em> and <em>Hyphtis suaveolens</em> evaluated <em>in-vitro </em>on associated pathogens. Methods: Flora of blight-infected cashew seedlings was randomly collected from Cocoa Research Institute of Nigeria (CRIN) nursery between July and October, 2019. Mycoflora analysis was carried out in the plant pathology (Mycology) laboratory of CRIN. Antifungal assay of powdered <em>Mangifera indica</em>, <em>Azadirachta indica</em> and <em>Hyphtis suaveolens</em> were screened using aqueous extracts at 1:4 (w/v). Potato Dextrose Agar (PDA) amended with 1ml of 100%, 75%, 50%, 25%, and 0% of the extracts and Mancozeb (synthetic fungicide) as standard, 5mm mycelia mat disc of 10day old each of <em>Lasiodiplodia theobromae, Fusarium pallidoroseum and Macrophomina sp.</em> were placed at the centre of the amended media in triplicate and incubated 5-7days using complete randomized design (CRD). Mycelia extension inhibition and percentage growth inhibition (R) obtained. Results: <em>Aspergillus niger, A. flavus, Fusarium oxysporium, F. pallidoroseum, Lasiodiplodia theobromae., Pythium sp., Rhizopus sp., Macrophomina sp</em>. and <em>Rhizotonia sp</em>. were isolated. <em>Fusarium pallidoroseum, L. theobromae</em> and <em>Macrophomina </em>sp. screened with the varied concentrations of botanicals showed reduction in mycelia diameter; <em>Mangifera indica </em>(31.50%)<em>, A. indica</em> (48.70%) and H<em>. suaveolens </em>(25.86%) on <em>F. pallidoroseum</em> favorably competed with mancozeb (39%) at 25% concentration while only <em>M. indica</em> was significant on <em>L.theobromae(</em>64.12%)and <em>Macrophomina sp.</em>(40.29%) and significantly different from control (0%). Conclusion: Aqueous extracts of <em>M. indica, A. indica and H. suaveolens </em>showed fungicidal potential on <em>F. pallidoroseum </em>and <em>M. indica was</em> significant on <em>L. theobromae </em>and <em>Macrophomina sp</em>.
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Kasprowicz, Marek Jan, Magdalena Kozioł, and Anna Gorczyca. "The effect of silver nanoparticles on phytopathogenic spores of Fusarium culmorum." Canadian Journal of Microbiology 56, no. 3 (2010): 247–53. http://dx.doi.org/10.1139/w10-012.
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The aim of the present experiment was to investigate the influence of silver nanoparticles on Fusarium culmorum (W.G. Smith) Sacc. (FC) spores. The silver nanoparticles were produced by the high-voltage arc discharge method. To test the effect of silver nanoparticles on FC spores, 3 parameters were tested. One of these parameters was the vegetative mycelial growth in 2 experiments. The first involved the growth of FC spores on potato dextrose agar (PDA) medium after contact with 0.12–10 ppm of silver nanoparticles, and the second the growth of spores after contact with 0.12–2.5 ppm solutions of silver, but with culturing on 3 types of media (PDA, nutrient-poor PDA, and agar) instead. The next parameter was the formation of spores after the mycelia were cultured. The last parameter was spore germination in a 2.5 ppm solution of silver nanoparticles. A significant reduction in mycelial growth was observed for spores incubated with silver nanoparticles. This relationship was dependent on the incubation time and type of growth medium, but did not depend significantly on the concentration of silver nanoparticles up to 2.5 ppm. The sporulation test showed that, relative to control samples, the number of spores formed by mycelia increased in the culture after contact with silver nanoparticles, especially on the nutrient-poor PDA medium. The 24 h incubation of FC spores with a 2.5 ppm solution of silver nanoparticles greatly reduced the number of germinating fragments and sprout length relative to the control.
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Qiu, Junzhi, Yubin Su, Ivan Gelbǐc, Yunfeng Qiu, Xiaocong Xie, and Xiong Guan. "Proteomic analysis of proteins differentially expressed in conidia and mycelium of the entomopathogenic fungus Aschersonia placenta." Canadian Journal of Microbiology 58, no. 12 (2012): 1327–34. http://dx.doi.org/10.1139/w2012-111.
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The infection of insects by the entomopathogenic fungus Aschersonia placenta depends on conidia. To identify proteins differentially expressed in A. placenta conidia vs mycelia, we performed a comparative proteomic analysis of A. placenta using 2-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). We detected 1022 2-DE protein spots in conidia and 1049 in mycelia and analyzed 48 (13 from conidia and 35 from mycelia) using MALDI-TOF-MS. Finally, we identified 28 proteins (7 from conidia and 21 from mycelia). The identified proteins exclusive to conidia included major proteins participating in oxidation–reduction processes and vegetative insecticidal protein 1 (Vip1), a protein that is likely involved in pathogenicity. The identified proteins exclusive to mycelia were those involved in biosynthesis and metabolism, including uridine diphosphate galactopyranose mutase, which might play key roles in hyphal morphogenesis. This report provides the first proteomic analysis of different developmental stages of an Aschersonia species. Although only a small number of proteins were identified, the data represent a useful foundation for future studies concerning the molecular basis of entomopathogenicity in the species A. placenta and in the genus Aschersonia.
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Hidalgo-Sanz, Raquel, María-Ángeles Del-Castillo-Alonso, Susana Sanz, Carmen Olarte, Javier Martínez-Abaigar, and Encarnación Núñez-Olivera. "The Two Mycological Sides of Ultraviolet-B Radiation: Harmless for Mushroom Mycelia, Harmful for Mycopathogenic Mould Spores." Agriculture 14, no. 5 (2024): 681. http://dx.doi.org/10.3390/agriculture14050681.
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Mycopathogenic moulds are responsible for the greatest crop losses of cultivated mushrooms, thus having a significant negative economic impact on industry. Pesticides are the most common treatment against mycopathogenic moulds, but ultraviolet-B (UV-B, 280–315 nm) radiation could be a more ecological alternative. Thus, we studied the effect of UV-B (at doses from 8 to 192 kJ m−2) on four common mycopathogenic moulds (Cladobotryum mycophilum, Lecanicillium fungicola, Trichoderma aggressivum, and Mycogone perniciosa) under in vitro conditions, using four different culture media. UV-B was tremendously effective in inactivating mould spores even at the lowest dose, with the exception of those of T. aggresivum. Contrarily, UV-B did not present any effect on the development of the host mycelium (Agaricus bisporus), even at the highest dose, when cultivated on Compost Tea medium (CT). This is the most similar medium to the substrate used for commercial mushroom cultivation. UV-B reduced the mould mycelia development in a dose-response manner, but this reduction depended on the species, with the strongly pigmented T. aggressivum as the most tolerant species. Regarding the culture media, all of them (especially CT) absorbed UV-B intensely, contributing to the protection of the mycelia. Overall, UV-B radiation could constitute an ecologically friendly alternative to chemical treatments against mycopathogenic moulds, due to its capacity to inactivate their spores and (in some cases) their mycelia without affecting their hosts.
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PASTER, NACHMAN, MAZAL MENASHEROV, UZI RAVID, and BENJAMIN JUVEN. "Antifungal Activity of Oregano and Thyme Essential Oils Applied as Fumigants Against Fungi Attacking Stored Grain." Journal of Food Protection 58, no. 1 (1995): 81–85. http://dx.doi.org/10.4315/0362-028x-58.1.81.
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Essential oils from oregano and thyme were applied for 24 h as fumigants against the mycelia and spores ofAspergillus flavus, Aspergillus niger and Aspergillus ochraceus, as well as against natural microflora of wheat grains. The minimal inhibitory concentration (MIC) of oregano oil needed to inhibit the mycelial growth of the fungi was 2.0 μl/L, while spores were eradicated following exposure to 2.0 to 2.5 μl/L. The thyme essential oil was less efficient in controlling mycelia and growth was observed even following exposure to 4.0 μl/L. However, the thyme essential oil was fungitoxic to spores (MIC = 3.0 μl/L). In another set of trials the efficacy of the oils and two of their constituents (carvacrol and thymol) in controlling natural microflora of surface-sterilized wheat grain was studied. Of the four materials investigated, only oregano essential oil exhibited fungicidal activity and, following 24 h exposure to 20 μl/L, a significant reduction in the percent of infested grain was observed even after 5 days of incubation on potato dextrose agar. A reduction in the germinability of the grains was evident following exposure to the materials tested. When the fungicidal activity of oregano essential oil was evaluated using grains with different moisture contents (MC), data revealed that the better inhibitory effect was achieved in grain with a high MC. The findings emphasize the toxicity of oregano and thyme essential oils as fumigants against fungi attacking stored grain and strengthen the possibility of using them as an alternative to chemicals for preserving stored grains.
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Soltani-Horand, Parya, Hamideh Vaghari, Jahanbakhsh Soltani-Horand, Mohammad Adibpour, and Hoda Jafarizadeh-Malmiri. "Extracellular Mycosynthesis of Antibacterial Silver Nanoparticles Using Aspergillus flavus and Evaluation of their Characteristics." International Journal of Nanoscience 19, no. 02 (2019): 1950009. http://dx.doi.org/10.1142/s0219581x19500091.
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Fungi extracellular synthesis of silver nanoparticles (AgNPs) using Aspergillus flavus mycelia extract was evaluated. Fourier transform-infrared spectroscopy revealed the presence of hydroxyl and amide I groups in the prepared fungi mycelia extract which those had key roles in the reduction of silver ions and stabilizing of the formed AgNPs. Response surface methodology (RSM) has been used to optimize and evaluate the effects of the prepared mycelia extract amount (5–7[Formula: see text]mL) and autoclave heating time (10–20[Formula: see text]min) on the particle size of the synthesized AgNPs. Obtained results revealed that the spherical mono-dispersed AgNPs with high stability were synthesized using 6[Formula: see text]mL of fungal mycelia extract and hydrothermally heating for 15[Formula: see text]min. At this obtained optimum synthesis conditions, broad emission peaks ([Formula: see text], mean particle size, concentration and zeta potential values for the fabricated AgNPs were 450[Formula: see text]nm, 44[Formula: see text]nm, 72[Formula: see text]ppm and [Formula: see text][Formula: see text]mV, respectively. The synthesized AgNPs also indicated high antibacterial activity against both Gram positive and negative bacteria.
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Chang, Tao-Ho, Ying-Hong Lin, Yu-Ling Wan, Kan-Shu Chen, Jenn-Wen Huang, and Pi-Fang Linda Chang. "Degenerated Virulence and Irregular Development of Fusarium oxysporum f. sp. niveum Induced by Successive Subculture." Journal of Fungi 6, no. 4 (2020): 382. http://dx.doi.org/10.3390/jof6040382.
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Successive cultivation of fungi on artificial media has been reported to cause the sectorization, which leads to degeneration of developmental phenotype, and virulence. Fusarium oxysporum f. sp. niveum (Fon), the causal agent of watermelon Fusarium wilt, forms degenerated sectors after successive cultivation. In the present research, we demonstrated that subculture with aged mycelia increased the incidence of degenerations. To further investigate the differences between the Fon wild type (sporodochial type, ST) and variants (MT: mycelial type and PT: pionnotal type), developmental phenotypes and pathogenicity to watermelon were examined. Results in variants (PT2, PT3, PT11, and MT6) were different from ST with mycelia growth, conidia production and chlamydospore formation. Virulence of degenerated variants on susceptible watermelon Grand Baby (GB) cultivar was determined after inoculation with Fon variants and Fon ST. In root dipping methods, Fon variants showed no significant differences in disease progress compared with ST. Fon variants showed a significant decrease in disease progression compared with ST through infested soil inoculation. The contrasting results of two inoculation methods suggest that the degenerative changes due to repeated successive cultivation may lead to the loss of pathogen virulence-related factors of the early stage of Fon infection process. Therefore, cell wall-degrading enzymes (CWDEs; cellulase, pectinase, and xylanase) activities of different variants were analyzed. All Fon degenerated variants demonstrated significant decreases of CWDEs activities compared with ST. Additionally, transcript levels of 9 virulence-related genes (fmk1, fgb1, pacC, xlnR, pl1, rho1, gas1, wc1, and fow1) were assessed in normal state. The degenerated variants demonstrated a significantly low level of tested virulence-related gene transcripts except for fmk1, xlnR, and fow1. In summary, the degeneration of Fon is triggered with successive subculture through aged mycelia. The degeneration showed significant impacts on virulence to watermelon, which was correlated with the reduction of CWDEs activities and declining expression of a set of virulence-related genes.
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Das, Sumanjari, Dale R. Gardner, Marwa Neyaz, Allen B. Charleston, Daniel Cook, and Rebecca Creamer. "Silencing of the Transmembrane Transporter (swnT) Gene of the Fungus Slafractonia leguminicola Results in a Reduction of Mycotoxin Transport." Journal of Fungi 9, no. 3 (2023): 370. http://dx.doi.org/10.3390/jof9030370.
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Slafractonia leguminicola infects red clover and other legumes, causing black patch disease. This pathogenic fungus also produces two mycotoxins, slaframine and swainsonine, that are toxic to livestock grazing on clover hay or pasture infested with S. leguminicola. Swainsonine toxicosis causes locoism, while slaframine causes slobbers syndrome. The mechanism of toxin secretion by S. leguminicola is poorly understood. The aim of this research was to investigate the role of a putative transmembrane transporter, SwnT, in mycotoxin transport. The swnT gene was silenced by RNA interference using the silencing vector Psilent1, which included inverted repeat transgenes of swnT. This resulted in a significant reduction of swnT transcript levels compared with the controls. Silencing caused a decline in the active efflux of toxins from the mycelia to the media, as shown by LC–MS analysis. Transformants in which swnT was silenced showed higher concentrations of both toxins in the mycelia compared with the concentrations in the media. These transformants exhibited a visibly distinct phenotype with much thicker and shorter mycelia than in the wild type. These transformants were also unable to infect detached clover leaves, unlike the controls, suggesting that SwnT function may play an important role in pathogenesis in addition to mycotoxin transport. This research demonstrates the importance of this transporter to the secretion of mycotoxins for this phytopathogenic fungus.
Book chapters on the topic "Mycelia reduction"
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Vazquez, Jose A. "Rhodotorula, Malassezia, Trichosporon,and Other Yeast-Like Fungi." In Clinical Mycology. Oxford University PressNew York, NY, 2003. http://dx.doi.org/10.1093/oso/9780195148091.003.0013.
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Abstract Yeasts are found ubiquitously in nature, in association with plants, mammals, and insects. Accordingly, humans are continually exposed to multiple genera of yeasts via various routes. Depending on the interaction between host mucosal defense mechanisms and fungal virulence factors, yeast colonization may be transient or persistent, with either systemic or local disease. Yeast organisms are usually of low virulence, and frequently require a significant alteration or reduction in host defenses prior to tissue invasion. Recently, because of the increased population of immunocompromised patients, the frequency of yeast infections as well as the organisms causing disease continues to grow. Rhodotorulosis results from infection with the genus Rhodotorula. Although the yeast is recovered world-wide from a variety of sources, infection is generally only seen in the immunocompromised host. The fungi from the genus Rhodotorula are imperfect basidiomycetous yeast belonging to the family Crypto-coccaceae. There are 8 species in the genus Rhodotorula (Fell, 1984a; Kreger-van Rij, 1984). Rhodotorula rubra (R. mucilaginosa) is the species most frequently associated with human infection. The other less commonly isolated species include R. glutinis, R. pilimanae, R. pallida, R. aurantiaca, R. minuta (syn, R. marina). The majority of Rhodotorula species produce red-to-orange colonies due to the presence of carotenoid pigments (Rippon, 1988; Kwon-Chung and Bennett, 1992). The yeast is mucoid, encapsulated, rarely forms mycelia, and readily grows on almost all types of culture media. Rhodotorula is very similar to the Cryptococcus in rate of growth, cell size and shape, presence of capsule, and the ability to split urea. The difference includes Rhodotorula’s inability to assimilate inositol and ferment sugars (Kwon-Chung and Bennett, 1992). Rhodotorula can be differentiated from other red-pigmented yeast, such as Sporobolomyces, by the lack of ballistospore formation.
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Nath Gupta, Ramesh, Kishor Chand Kumhar, and J. N. Srivastava. "Macrophomina Phaseolina: An Agriculturally Destructive Soil Microbe." In Industrial Applications of Soil Microbes. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815050264123020015.
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Macrophomina phaseolina (Tassi) Goid. is a destructive fungal soil microbe, a cause of charcoal rot disease and causes heavy losses in agricultural production. It is non-specific and appears in moderate to severe form every year worldwide. Due to the seriousness and economic importance of the pathogen as well as disease, it requires multiple approaches like epidemiological study, induction of systemic resistance through non-conventional chemicals, host-pathogen resistance and chemical as well as phytoextract application for its management. Epidemiological studies reveal that the onset of charcoal rot varied in different varieties during different dates of sowing. Timely sowing of crops is an important tool for reducing disease incidence. The intensity of disease in a timely sown crop is less, with higher production and productivity. The non-conventional chemicals like salicylic acid, acetylsalicylic acid, indole acetic acid, indole butyric acid, riboflavin, and thiamine induce systemic acquired resistance (SAR) and effectively inhibit mycelial growth of the pathogen. These non-conventional chemicals showed a reduction of charcoal rot disease under field conditions. It also enhances the yield-attributing traits and yield. It induces total phenol content, peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, and catalase activity by the treatment of these chemicals. These activities showed a differential reaction after inoculation of the pathogen on different varieties. However, resistant varieties showed higher induction of biochemical activities than susceptible ones. Different phytoextracts showed inhibition of mycelial growth and a reduction of disease incidence in different crops. Seed treatment with fungicides is an effective method for controlling the pathogen and ultimately enhances the production of the crop. Genotype evaluation for host resistance is an effective, economical, and continuous way of managing the pathogen and disease.
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Selwal, Krishan K., Garima Deswal, Harsha Nirvan, and Manjit K. Selwal. "Green Synthesis of Nanoparticles using Fungal Extracts." In Mycology: Current and Future Developments. BENTHAM SCIENCE PUBLISHERS, 2022. http://dx.doi.org/10.2174/9789815051360122030008.
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Nanotechnology involves the synthesis of nanoparticles (NPs) and paved the way for the possibility of applications in different fields such as pharmaceutical science, industry, environment and biosensor technology. The metal nanoparticles synthesis using fungal extract is gaining momentum due to their novel chemical, optical, electrical, and magnetic properties. The mycelial biomass is found to be more resistant against pH, temperature, agitation and pressure compared to bacterial and plant extract and thus more appropriate for industrial production. The nano-sized particles synthesized by green chemistry are of better quality than the ones made by chemical reduction methods such as laser ablation, metallic wire explosion, photochemical or radiation reduction and sonochemical method. The chemical methods can pose a risk to environmental and animal health due to release of the hazardous toxic component. Therefore, nanoparticles synthesis using fungal extract could be an ecofriendly alternative to chemical-based methods as green synthesis has the lesser possibility of such component release. The fungal extract comprises a plethora of secreted extracellular proteins, enzymes, vitamins and ions which are responsible for the reduction and stability of nano-size metallic particles. The biogenic nanoparticles thus produced have attained much interest due to their composition, shape and size, photochemical, optical and chemical properties. The nanomaterials have applications in various fields such as biosensor technology, DNA based techniques, metabolomics, antimicrobial agents, cancer cell treatment, protein engineering, purification of water and degradation of pesticides, synthetic biology, downstream processing and delivery of therapeutic compounds.
Conference papers on the topic "Mycelia reduction"
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"Sweet orange essential oil-based chitosan emulsions against corn seed-borne pathogens." In Plant Health 2024. American Phytopathological Society, 2024. http://dx.doi.org/10.1094/aps-ph24-007.
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The control of plant diseases with emulsions produced from agro-industry residues has recently gained attention. The objective of this work was to evaluate effects of chitosan-based emulsions containing orange essential oil (OEO) on the in vitro growth and sporulation of Fusarium verticillioides, Acremonium sp., Penicillium sp. and Aspergillus sp. isolated from corn seeds. Emulsions (0, 5, 10, 20% w/v) were added to PDA medium and the mixture was dispensed into Petri dishes and kept in the dark. Mycelial disks were transferred to the plates and stored at 22 ± 1°C under a 12-hour photoperiod for 9, 10, 16 and 11 days, respectively for each fungus. The results were expressed as the mean colony diameter and percentage of inhibition. On the 9th, 10th, 16th and 11th day, respectively, a suspension was obtained by scraping the mycelium with distilled water, followed by gravity filtration, and spore count was performed using a hemocytometer. Increasing concentration of OEO in the chitosan solution led to a progressive reduction in the diameter of colonies of all fungi. The maximum fungistatic effect was observed with 20% OEO, with mycelial growth inhibitions of 76.6%, 68.0%, 68.7% and 80.5%, respectively. Sporulation was practically absent for Penicillium sp. and Acremonium sp., regardless of the concentration. For F. verticillioides and Aspergillus sp., the reduction in the number of spores was dose-dependent. The results gathered in this study indicate the great potential of chitosan-based emulsions with OEO towards a more sustainable agriculture.