Academic literature on the topic 'Mycelial growth and mycelial colonization'
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Journal articles on the topic "Mycelial growth and mycelial colonization"
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Kumakech, Alfred, and Tonny Opio. "Culture media influence on vegetative growth and mycelia weight of Mycosphaerella fijiensis: implication for inoculum production." Brazilian Journal of Science 2, no. 7 (2023): 103–7. http://dx.doi.org/10.14295/bjs.v2i7.356.
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Six (6) culture media (Potato Dextrose Agar, Malt Extract Agar, AFPA Base, Czapek Dox Agar, Nutrient Agar and Yeast Extract), were tested for their effect on colony growth and mycelia weight of Mycosphaerella fijiensis. The isolate of M. fijiensis (Kaw10) produced vegetative mycelial growth on all six-culture media. Culture media effect on the level of vegetative mycelial colonization and mycelia weight was significant. The highest and lowest vegetative mycelia colonization was recorded on malt extract (23.5 mm) and AFPA Base (4.2 mm), respectively. Similarly, culture media effect on mycelia weight was significant. Malt extract agar produced more mycelia weight (0.34g) than the control, PDA (0.11g). Collectively, our data identify malt extract agar as a good medium for improving growth of Mycosphaerella fijiensis.
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Amir, N. F., A. Mohd Aris, A. Mohamad, N. A. Umor, S. Abdullah, and F. Z. Mohd Yusof. "Mycelium running of volvariella volvacea on palm oil empty fruit bunch fibre following different substrate and mycelium treatment." IOP Conference Series: Earth and Environmental Science 1151, no. 1 (2023): 012054. http://dx.doi.org/10.1088/1755-1315/1151/1/012054.
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Abstract Leading the palm oil industry, Malaysia strives to become the powerhouse of palm oil producers. However, it is crucial that the industry does not influence the environment negatively in any way. Thus, reusing and repurposing palm oil residues in mushroom farming is one of the potential solutions for agricultural waste management. In this study, the objective of this study is to determine the mycelial performance of V. volvacea in palm oil empty fruit bunch (EFB) fibre as substrate and to evaluate the mycelial growth performance of V. volvacea in two different substrate treatment. The substrate treatment involved the non-treated EFB fibres (N substrate) and EFB fibres treated with 10% Calcium Carbonate (CaCO3) (T substrate), and mycelium treatment involved in gamma-irradiated mycelium (G sample) and non-irradiated mycelium (NG sample). Results shows that mycelium inoculated on treated EFB fibres exhibited a higher growth rate compared to non-treated EFB fibres. The full colonization days of mycelial run-on substrate using treated substrate was found reduced from 15 days to 9 days, as compared to the non-treated substrate. However, gamma irradiated mycelium was found giving no significant changes to the rate of mycelial run. In conclusion, substrate treated with CaCO3, increased the growth of mycelium. In future, it is suggested to measure the effect of irradiation using different gamma irradiation exposure.
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Joie, Ambos A. Allysa, and L. Alberto Ambos. "Multiplication of Oyster Mushroom Mother Spawn (Pleurotus Ostreatus L.) Using Different Boiling Periods of Sorghum." American Journal of Agricultural Science, Engineering, and Technology 6, no. 3 (2022): 78–82. http://dx.doi.org/10.54536/ajaset.v6i3.855.
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The study was focused on the multiplication of oyster mushroom mother spawn using different boiling periods of sorghum. It was conducted at the Mushroom Laboratory of the Department of Plant Science – College of Agriculture, Mindanao State University Main Campus, Marawi City, from March 31, 2022, to April 21, 2022. The length of mycelia of each treatment is measured every five (5) days for the expansion of mycelia inside the bottles of sorghum. The ANOVA was the statistical tool that was used in analyzing the data. The results revealed that (1) there is a significant difference in the number of days from inoculation to colonization of substrate in every treatment; (2) Treatment 1 has the slowest mycelial growth in the bottle; (3) Treatment 2 has the fastest mycelial growth in the bottle; (4) there is a level of 10% significant difference in the length of mycelial growth in every treatment on the third data gathering; (5) Treatment 1 (fifteen-minute boiling period) has the slowest colonization of the sorghum as mother spawn substrate in the bottle, (6) Treatment 2 (thirty-minute boiling period) has the fastest colonization of the sorghum as mother spawn substrate in the bottle, and (7) Treatment 2 (thirty-minute boiling period) has the best boiling period of the substrate for mother spawn multiplication.
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Joie, Ambos A. Allysa, and Ambos L. Alberto. "Multiplication of Oyster Mushroom Mother Spawn (Pleurotus Ostreatus L.) Using Different Boiling Periods of Sorghum." American Journal of Agricultural Science, Engineering, and Technology 6, no. 3 (2022): 78–82. https://doi.org/10.54536/ajaset.v6i3.855.
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The study was focused on the multiplication of oyster mushroom mother spawn using different boiling periods of sorghum. It was conducted at the Mushroom Laboratory of the Department of Plant Science – College of Agriculture, Mindanao State University Main Campus, Marawi City, from March 31, 2022, to April 21, 2022. The length of mycelia of each treatment is measured every five (5) days for the expansion of mycelia inside the bottles of sorghum. The ANOVA was the statistical tool that was used in analyzing the data. The results revealed that (1) there is a significant difference in the number of days from inoculation to colonization of substrate in every treatment; (2) Treatment 1 has the slowest mycelial growth in the bottle; (3) Treatment 2 has the fastest mycelial growth in the bottle; (4) there is a level of 10% significant difference in the length of mycelial growth in every treatment on the third data gathering; (5) Treatment 1 (fifteen-minute boiling period) has the slowest colonization of the sorghum as mother spawn substrate in the bottle, (6) Treatment 2 (thirty-minute boiling period) has the fastest colonization of the sorghum as mother spawn substrate in the bottle, and (7) Treatment 2 (thirty-minute boiling period) has the best boiling period of the substrate for mother spawn multiplication.
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Joie, Ambos A. Allysa, and Ambos L. Alberto. "Multiplication of Oyster Mushroom Mother Spawn (Pleurotus Ostreatus L.) Using Different Boiling Periods of Sorghum." American Journal of Agricultural Science, Engineering, and Technology 6, no. 3 (2022): 78–82. https://doi.org/10.5281/zenodo.7451362.
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The study was focused on the multiplication of oyster mushroom mother spawn using different boiling periods of sorghum. It was conducted at the Mushroom Laboratory of the Department of Plant Science – College of Agriculture, Mindanao State University Main Campus, Marawi City, from March 31, 2022, to April 21, 2022. The length of mycelia of each treatment is measured every five (5) days for the expansion of mycelia inside the bottles of sorghum. The ANOVA was the statistical tool that was used in analyzing the data. The results revealed that (1) there is a significant difference in the number of days from inoculation to colonization of substrate in every treatment; (2) Treatment 1 has the slowest mycelial growth in the bottle; (3) Treatment 2 has the fastest mycelial growth in the bottle; (4) there is a level of 10% significant difference in the length of mycelial growth in every treatment on the third data gathering; (5) Treatment 1 (fifteen-minute boiling period) has the slowest colonization of the sorghum as mother spawn substrate in the bottle, (6) Treatment 2 (thirty-minute boiling period) has the fastest colonization of the sorghum as mother spawn substrate in the bottle, and (7) Treatment 2 (thirty-minute boiling period) has the best boiling period of the substrate for mother spawn multiplication.
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Chang, Wanqiu, Weilin Feng, Yang Yang, et al. "Metagenomics analysis of the effects of Agaricus bisporus mycelia on microbial diversity and CAZymes in compost." PeerJ 10 (December 7, 2022): e14426. http://dx.doi.org/10.7717/peerj.14426.
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Agaricus bisporus growth alters the lignocellulosic composition and structure of compost. However, it is difficult to differentiate the enzyme activities of A. bisporus mycelia from the wider microbial community owing to the complication of completely speareting the mycelia from compost cultures. Macrogenomics analysis was employed in this study to examine the fermentation substrate of A. bisporus before and after mycelial growth, and the molecular mechanism of substrate utilization by A. bisporus mycelia was elucidated from the perspective of microbial communities and CAZymes in the substrate. The results showed that the relative abundance of A. bisporus mycelia increased by 77.57-fold after mycelial colonization, the laccase content was significantly increased and the lignin content was significantly decreased. Analysis of the CAZymes showed that AA10 family was extremely differentiated. Laccase-producing strains associated with AA10 family were mostly bacteria belonging to Thermobifida and Thermostaphylospora, suggesting that these bacteria may play a synergistic role in lignin decomposition along with A. bisporus mycelia. These findings provide preliminary evidence for the molecular mechanism of compost utilization by A. bisporus mycelia and offer a reference for the development and utilization of strains related to lignocellulose degradation.
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De, Guzman Ronel S. "Evaluation of Mycelial Growth of Oyster Mushroom (Pleurotus ostreatus) from Cassava and Taro Pure Culture Media in Crack Corn." JournalNX- A Multidisciplinary Peer Reviewed Journal 7, no. 4 (2021): 177–83. https://doi.org/10.5281/zenodo.4764661.
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The study aimed to evaluate mycelial growth of cassava + agar and taro + agar OM pure culture in cracked corn subculture media. Pure culture from cassava + agar (treatment 1) and taro + agar (treatment 2) were used in the inoculation of subculture media. The data gathered was compared using T-test. The evaluation of the first mycelial growth appearance from inoculation in two treatments exhibited significant difference with an average of 1.33-day period in treatment 1 and 2.33-day period in treatment 2. The analysis in number of days from inoculation to full mycelial colonization also showed significant differences. The shortest time was recorded in treatment 1 with average of 9.53-day period followed with an average of 10.76-day period for treatment 2. The result confirmed the result of Stanley and Nyenke (2011) that the cassava stimulated luxuriant mycelial growth rate and extension. The use of cassava + agar pure culture in oyster mushroom subculture production was recommended for its faster response to the cracked corn subculture media due to its fast mycelial growth.
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Petraglia, Tania, Tiziana Latronico, Grazia Maria Liuzzi, Angela Fanigliulo, Aniello Crescenzi, and Rocco Rossano. "Hydrolytic Enzymes in the Secretome of the Mushrooms P. eryngii and P. ostreatus: A Comparison Between the Two Species." Molecules 30, no. 12 (2025): 2505. https://doi.org/10.3390/molecules30122505.
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The fungi belonging to the genus Pleurotus can be cultivated in different substrates and represent excellent producers of several extracellular enzymes. In this study, we analyzed eleven hydrolytic enzymes of the P. eryngii and P. ostreatus secretomes, which were collected at three different growth stages after 23 days (mycelial colonization of about 50% of the substrate), 34 days (100% colonization of the substrate) and 50 days (after the first flush). Mushrooms were axenically cultivated on the same substrate. The results demonstrate that proteases, lipases, amylases, α-glucosidase, cellulases (endoglucanase, β-cellobiohydrolase and β-glucosidase) and hemicellulase (xylosidase, glucuronidase, arabinosidase and mannosidase) activities were higher in the secretomes from P. eryngii than those from P. ostreatus. Time course analysis revealed for both species a similar enzymatic activity profile, in which in the early stages of mycelium development, both species use starch as the main carbon source. Protease and lipase activities increased and remained constant during the subsequent formation of fruiting bodies, whereas cellulase and hemicellulase activities decreased after the complete mycelial colonization of the substrate. The zymographic analysis suggested the presence in the secretomes of proteolytic activities belonging to different classes. In conclusion, both mushroom species released into the secretomes a broad spectrum of hydrolytic enzymes potentially useful in various biotechnological fields.
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Bastide, Paul Y. de la, Yves Piché, and Bradley R. Kropp. "Vegetative interactions among mycelia of Laccaria bicolor in pure culture and in symbiosis with Pinus banksiana." Canadian Journal of Botany 73, no. 11 (1995): 1768–79. http://dx.doi.org/10.1139/b95-189.
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Mycelial interactions of Laccaria bicolor strains were studied in pure culture and after inoculation onto mycorrhizal hosts. Monokaryon–monokaryon and dikaryon–monokaryon crosses were performed on an agar medium that enhanced nuclear migration to study mating events. The first observance of clamped hyphae, their location, evidence of nuclear migration, and the occurrence of dikaryon–monokaryon matings varied among crosses. One monokaryon–monokaryon and two dikaryon–monokaryon combinations were selected for seedling inoculation to compare their mycorrhizosphere interactions with those observed on agar medium. Seedlings of Pinus banksiana were grown for 20 weeks in a mycelium inoculated soil medium. Three seedlings from each treatment were selected at harvest and ectomycorrhizae reisolates were subject to randomly amplified polymorphic DNA analysis to identify genotypes. This analysis was also done for mycelial samples of the same crosses on agar medium. Variation in the mycobiont genotype was observed for different root isolates from the same seedling, which had been initially inoculated with a compatible monokaryon–monokaryon or a dikaryon–monokaryon strain combination. Root isolates from seedlings receiving the latter treatment included a new dikaryotic genotype produced by a dikaryon–monokaryon mating. Seedling growth was reduced with ectomycorrhizal colonization, most likely because of the photosynthate requirements of the mycobiont during this study. The nature of mycelial interactions and the potential value of a genetically variable mycobiont are discussed. Key words: Buller phenomenon, ectomycorrhizae, intraspecific variability, mycelial interactions, RAPD analysis.
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Zhou, Jun-Liang, Shuang Song, Zhen-Xing Huang, et al. "Cultivation of Pleurotus ostreatus, a potential candidate for biogas residues degradation." BioResources 13, no. 3 (2018): 5432–49. http://dx.doi.org/10.15376/biores.13.3.5432-5449.
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Biogas projects are rapidly expanding in China, but there is insufficient cropland to degrade these biogas residues. Mushroom cultivation has been used to degrade various agricultural wastes. In this study, to screen the feasibility of utilizing biogas residues as potential substrates for the cultivation of Pleurotus ostreatus, seven different concentrations (0 to 60%) of biogas residue mixed with cottonseed hull, wheat bran, and lime were used to cultivate P. ostreatus. The mycelial growth rate, mycelial colonization time, yield, biological efficiency, chemical compositions, and content of four heavy metals, Cd (cadmium), Pb (lead), Hg (mercury), and As (arsenic), were analyzed. The results showed that 10 to 30% of biogas residue mixed in the substrates induced the growth of P. ostreatus mycelia faster than the others. A lower percentage (10 to 20%) of biogas residue added to the substrates is beneficial to the production and nutrient components of P. ostreatus, and the fruiting bodies produced on biogas residue-containing substrates conform to the safety standards for edible mushrooms. Although the total harvest is not significantly increased when biogas residue is added, the utilization of cheap biogas residues can conceivably reduce the practical cost and benefit the environment.
Dissertations / Theses on the topic "Mycelial growth and mycelial colonization"
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Evans, Jeannette M. "The control effects of ice crystal growth in a mycelial system." Thesis, University of Nottingham, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294730.
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Smith, Jeffrey Francis. "Factors affecting the selectivity of composts suitable for the cultivation of Agaricus species." Thesis, King's College London (University of London), 1994. https://kclpure.kcl.ac.uk/portal/en/theses/factors-affecting-the-selectivity-of-composts-suitable-for-the-cultivation-of-agaricus-species(55164b49-ac7a-4148-ab9a-982fb3ebada2).html.
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Ochoa, Sergio Huerta. "Modelling aspects of mycelial growth and citric acid formation in batch submerged culture." Thesis, University of Reading, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359386.
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Aynsley, M. "Modelling and simulation of mycelial growth in submerged culture with application to the fed-batch penicillin fermentation." Thesis, University of Newcastle Upon Tyne, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316130.
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Rosa, Janicéli. "Seleção de genótipos de guandu para resistência a Macrophomina phaseolina e esporulação do fungo /." Jaboticabal : [s.n.], 2006. http://hdl.handle.net/11449/96898.
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Resumo: Objetivou-se o ajuste de metodologia e seleção de genótipos de guandu para resistência a Macrophomina phaseolina a partir de material obtido pela Embrapa Pecuária Sudeste, e verificar o desenvolvimento micelial e esporulação do fungo em meios de cultura. O trabalho foi conduzido em casa de vegetação na UNESP/Jaboticabal no período de agosto de 2004 a dezembro de 2005. Para o ajuste de metodologia e seleção de genótipos resistentes ao fungo as sementes foram submetidas a escarificação com lixa d'água e inoculação artificial através do método de exposição das mesmas ao patógeno por diferentes períodos, que variaram de O a 72 horas. Foram avaliadas porcentagem de plantas sobreviventes e massa fresca. Já para o crescimento micelial e esporulação do fungo foi utilizado o método de sobreposição de discos de diferentes hospedeiros no meio de cultura. A escarificação das sementes contribuiu para a penetração do fungo nas mesmas o período de 24h de exposição das sementes ao fungo são suficientes para detectar diferenças no grau de resistência dos genótipos. Os genótipos mais resistentes são g167-97, g124-95, g27-94, g40-95, g154-95, g127-97 e g9m-97, e os mais suscetíveis são g48-95, g123-95, g8-95, g168-99 e g1m-95. A sobreposição de discos foliares de guandu em meio BDA e folha de papel de filtro em meio sojinha proporcionam um incremento na esporulação de M. phaseolina.<br>Abstract: This work had the objective of determining the best schedule for artificial inoculation and select pigeon pea genotypes resistant to Macrophomina phaseolina in material obtained by Embrapa Pecuária Sudeste, and verify the mycelial growth and sporulation of the fungi in middle of culture. The work were carried in greenhouse at the UNESP/Jaboticabal, from August 2004 to December 2005. For the methodology and selection adjustment of resistant genotypes to the fungi the seeds were submitted scarified with water sandpaper and artificial inoculation the seeds were the contact method to fungi for different periods, which varied from O to 72 hours. They were evaluated percentage of surviving plants and fresh mass. For the mycelial growth and sporulation of the fungi was used the superposition of disks method of different hosts in the middle of culture. The scarified of the seeds contributed for penetration of the fungi at the seeds; the period of 24h of contact of the seeds to the fungi enough to detect differences in the resistance degree ofthe genotypes. The genotypes g167-97, g124-95, 927-94, g40-95, g154-95, g127-97 and g9m-97 were found to be the most resistant and most susceptible were g48-95, g123-95, g8-95, g168-99 and g1m-95. The treatment with superposition of the leaf disks of pigeon pea in BDA and disks of filter paper in middle of soybean extract were the treatments that provided better sporulation levei in the conditions of that experiment were half.<br>Orientador: Rita de Cássia Panizzi<br>Coorientador: Rodolfo Godoy<br>Banca: Antonio de Goes<br>Banca: Patrícia Menezes Santos<br>Mestre
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Fonseca, Tamiris Rio Branco da. "Pleurotus ostreatoroseus DPUA 1720: avaliação do crescimento, produção de basidioma e determinação da atividade proteolítica em resíduos agroindustriais." Universidade Federal do Amazonas, 2013. http://tede.ufam.edu.br/handle/tede/4291.
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Previous issue date: 2013-05-02<br>CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Mushrooms arouse interest for presenting high nutritional and medicinal value. The representatives of the genus Pleurotus are able to grow in different agricultural and agro-industrial waste, since they secrete numerous enzymes that hydrolyze the substrates prepared from waste providing nutrient The search for alternative substrates increased in recent years due to the availability of regional waste little or never used in the production of edible mushrooms. The use of waste in solid-state fermentation has emerged also in the production of enzymes. Proteases are a group of enzymes commercially exploited, however the use of Basidiomycetes as producers of proteases is still a relatively unexplored. The aim of this study was to evaluate the mycelial growth as well as to investigate the production and partially characterize extracellular proteases from Pleurotus ostreatoroseus DPUA 1720 in different agro-industrial waste, selecting a mixture for the production of the mushroom and check their microbiological quality and nutritional value. Cultures were prepared in potato dextrose added yeast extract 0.5% (w/v). The vertical mycelial growth and production of proteases from P. ostreatoroseus DPUA 1720 were performed in agro-industrial waste (acai seed, sawdust, cupuassu bark, rice bran, bark and pineapple crown) for 15 days under two growing conditions (presence and absence of light). The production of basidiomata was performed in the substrate which presented satisfactory mycelial growth and vigorous mycelium. From this bioprocess evaluated the formation and development of early basidiomata, total time of cultivation, biological efficiency, productivity, production rate, loss of organic matter, microbiological and nutritional value of the mushroom. P. ostreatoroseus DPUA 1720 grew in all industrial residues also tested serving as sources for the production of proteases. In bark cupuaçu added rice bran mycelium was strongly vigorous and the mycelial growth satisfactory, therefore selected for the production of P. ostreatoroseus DPUA 1720. Under the experimental conditions the proteolytic activity was determined in crude extracts from the substrates of vertical mycelial growth with maximum proteolytic activity (7.89 U/mL) in bark cupuassu added rice bran in the presence of light. This substrate was the most efficient for the production of proteases with activity atpH 6.0 and optimum temperature at 40 ˚C. The basidiomata showed no contaminants, low in fat, high in fiber and protein, macro and micro minerals, essential and non-essential amino acids, may therefore be inserted into the feed as a safe and nutritious food.<br>Os cogumelos despertam interesse por apresentar alto valor nutricional e medicinal. Os representantes do gênero Pleurotus são capazes de crescer em diferentes resíduos agrícolas e agroindustriais, pois secretam inúmeras enzimas que hidrolisam os substratos elaborados a partir dos resíduos disponibilizando nutrientes. A busca por substratos alternativos cresceu nos últimos anos em virtude da disponibilidade de resíduos regionais pouco ou nunca utilizados na produção de cogumelos comestíveis. O uso de resíduos na fermentação semi-sólida vem despontando também na produção de enzimas. As proteases são o grupo de enzimas mais exploradas comercialmente, no entanto o uso de Basidiomicetos como produtores de proteases ainda é um tema pouco explorado. O objetivo deste trabalho foi avaliar o crescimento micelial, bem como investigar a produção e caracterizar parcialmente proteases extracelulares de Pleurotus ostreatoroseus DPUA 1720 em diferentes resíduos agroindustriais, selecionar uma mistura para produção dos basidiomas e verificar sua qualidade microbiológica e valor nutricional. As culturas foram preparadas em ágar batata dextrose acrescido de extrato de levedura 0,5% (p/v). O crescimento micelial vertical e a produção de proteases de P. ostreatoroseus foram realizados em resíduos agroindustriais (semente de açaí, serragem, casca de cupuaçu, farelo de arroz, casca e coroa de abacaxi) durante 15 dias, sob duas condições de cultivo (presença e ausência de luz). A produção do basidioma foi realizada no substrato que apresentou crescimento micelial satisfatório e micélio vigoroso. A partir desse bioprocesso foi avaliada a formação e desenvolvimento dos primórdios, tempo total de cultivo, eficiência biológica, produtividade, taxa de produção, perda de matéria orgânica, qualidade microbiológica e valor nutricional dos basidiomas. P. ostreatoroseus DPUA 1720 cresceu em todos os resíduos agroindustriais testados, também servindo como fontes para a produção de proteases. Em casca de cupuaçu adicionado de farelo de arroz o micélio foi fortemente vigoroso e o crescimento micelial satisfatório, por isso selecionado para produção de P. ostreatoroseus DPUA 1720. Nas condições experimentais a atividade proteolítica foi determinada nos extratos brutos provenientes dos substratos miceliados do crescimento micelial vertical com máxima atividade proteolítica (7,89 U/mL) em casca de cupuaçu adicionado de farelo de arroz na presença de luz. Este substrato foi o mais eficiente para a produção das proteases com atividade em pH 6,0 e temperatura ótima a 40˚C. Os basidiomas apresentaram ausência de contaminantes, baixo teor de lipídios, alto teor de fibras e proteínas, macro e microminerais, aminoácidos essenciais e não essenciais, podendo assim ser inserido na alimentação como um alimento saudável e nutritivo.
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Lima, Marcus Vinícius Lopes de. "Atividade do óleo volátil, fases e extrato etanólico de Piper aduncum L. contra Corynespora cassiicola (Berk. e M.A. Curtis) C.T. Wei, agente causal da mancha-alvo do tomateiro (Lycopersicon esculentum Mill.)." Universidade Federal do Amazonas, 2012. http://tede.ufam.edu.br/handle/tede/2670.
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Previous issue date: 2012-08-31<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The dark-brown spot is a disease that occurs in the leaves of the tomato tree and is caused by the fungus Corynespora cassiicola. The fungus is considered cosmopolitan and nonspecific because of the wide host range and geographical distribution. Due to the absence of resilient and grow commercial chemicals registered in Brazil to control the disease the same is done with the use of alternative products so healing. This paper reports the in vitro antifungal effect of the ethanol extract phases and volatile oil from Piper aduncum and prophylactic and curative effect of aqueous extract of P. aduncum in tomato seedlings. The aqueous extract was obtained by macerating 300 g of fresh leaves in 2 L of water, ethanol extract by means of cold maceration of leaves with ethanol, the essential oil obtained by the method of hydrodistillation of the dry leaves, the the hexane, chloroform, N-butanol and hydroalcoholic through the fractionation of the ethanol extract by the process of liquid-liquid partition. For in vitro assays were performed in petri dishes, the antifungal effect of the ethanol extract phases and volatile oil of P. aduncum on mycelial growth, and germination of conidia blades while in vivo via inoculation of conidia suspension of 104 conidia mL-1 in tomato seedlings of the cultivar Santa Cruz Kada, the analyzes were done before and after treatment with aqueous extract, evaluations of prophylactic and curative effect, respectively. The volatile oil of P. aduncum showed no inhibition on the mycelial growth of C. cassiicola concentrations tested. The ethanol extract, the hexane and chloroform showed antifungal effect in 10000 μg.mL-1 concentration on mycelial growth and spore germination. The minimum inhibitory concentration on the mycelial growth was in 2000 μg.mL-1 of the hexane phase, this same concentration was found for the germination of conidia in the ethanol extract. The aqueous extract of P. aduncum the test of curative effect at dosages 1:1 and 1:2, showed significant effect in reducing the severity of the disease. The calculation of AUDPC confirming curative effect at all doses tested. In testing the prophylactic aqueous extract showed no effectiveness in controlling the disease<br>A mancha alvo do tomateiro é uma doença que ocorre nas folhas e é causada pelo
fungo Corynespora cassiicola. O fungo é considerado cosmopólita e inespecífico devido
à ampla gama de hospedeiros e distribuição geográfica. Devido à inexistência de
cultivarem comerciais resistentes e produtos químicos registrados no Brasil para o
controle da doença, o mesmo é feito com uso de produtos alternativos de forma
curativa. Este trabalho relata o efeito antifúngico in vitro do extrato etanólico, fases e
óleo volátil de Piper aduncum e o efeito curativo e profilático do extrato aquoso de P.
aduncum em mudas de tomateiro. O extrato aquoso foi obtido por meio da maceração
de 300 g de folhas verdes em 2 L de água; O extrato etanólico, por meio da maceração
a frio de folhas secas com etanol; o óleo volátil obtido pelo método da hidrodestilação
das folhas secas; as fases hexânica, clorofórmica, N-butanólica e hidroalcóolico por
meio do fracionamento do extrato etanólico pelo processo de partição líquido-líquido.
Para as análises in vitro, foram realizadas em placas de petri o efeito antifúngico do
extrato etanólico, fases e óleo volátil de P. aduncum sobre o crescimento micelial, e em
lâminas a germinação dos conídios, enquanto que, in vivo, via inoculação de suspensão
de inóculo na concentração de 104
conídios.mL-1
em mudas de tomateiro do cultivar
Santa Cruz Kada, as análises foram feitas antes e após tratamento com extrato aquoso,
nas avaliações do efeito curativo e profilático, respectivamente. O óleo volátil de P.
aduncum não apresentou nenhuma inibição sobre o crescimento micelial de C.
cassiicola nas concentrações testadas. O extrato etanólico, fases hexânica e
clorofórmica apresentaram efeito antifúngico na concentração 10000 µg.mL-1
sobre o
crescimento micelial e germinação dos conídios. A concentração inibitória mínima sobre
o crescimento micelial foi em 2000 µg.mL-1
da fase hexânica, nesta mesma
concentração foi encontrado para a germinação dos conídios no extrato etanólico. O
extrato aquoso de P. aduncum no teste de efeito curativo nas dosagens 1:1 e 1:2,
apresentaram efeito significativo na redução da severidade da doença. O cálculo da
AACPD confirma o efeito curativo em todas as dosagens testadas. No teste profilático o
extrato aquoso não apresentou nenhuma efetividade no controle da doença
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Sousa, Francy Mary Galúcio. "Caracterização morfofisiológica de Corynespora cassiicola (Berk. e Curt.) Wei isolado de três hospedeiros no Amazonas." Universidade Federal do Amazonas, 2010. http://tede.ufam.edu.br/handle/tede/2683.
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Previous issue date: 2010-11-30<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Corynespora cassiicola has been related causing damages in several crops of economic importance in Amazonas State, principally Solanaceae and Cucurbitaceae families affecting production by significative form. This study aimed to characterize C. cassiicola isolates through colony morphology, sporulation, mycelial growth and spore dimension on culture media PDA, PSA, OEA and CA. Five mm-diameter disks taken from colonies grown on PDA medium for 15 days were transferred to Petri dishes containing each medium under room temperature and continuous light. The study was installed using randomized block design in a factorial 4 x 5 x 3 (culture media x isolates x hosts) with five replications. The effects of culture media varied to isolates of same host and among different hosts. Mycelial growth was observed in CA. The most spore production was observed in PDA and PSA media. A high variation of aspect and colonies coloration was observed among same host isolates and isolates of different hosts in all culture media. Variation trough spore dimension of C. cassiicola isolates was observed in all culture media tested.<br>O fungo Corynespora cassiicola já foi relatado causando danos em diversas culturas de interesse comercial no Estado do Amazonas, principalmente das famílias Solanaceae e Cucurbitaceae, afetando de forma significativa a produção. Este estudo teve como objetivo caracterizar isolados de C. cassiicola quanto a morfologia das colônias, esporulação, crescimento micelial e dimensão dos conídios nos meios de cultura BDA, BSA, AvA e CA. Discos de 5mm de diâmetro retirados de colônias crescidas em meio BDA por 15 dias, foram transferidos para o centro de placas de Petri contendo os meios de cultura e mantidas à temperatura ambiente sob luz contínua. O estudo seguiu esquema fatorial 4 x 5 x 3 (meios de cultura x isolados x hospedeiros) com cinco repetições. Os efeitos dos meios de cultura variaram para isolados do mesmo hospedeiro e entre hospedeiros diferentes. Maior crescimento micelial foi observado no meio CA. A maior produção de esporos foi observada nos meios BDA e BSA. Foi verificado uma ampla variação de aspecto e coloração das colônias entre os isolados do mesmo hospedeiro e entre isolados de hospedeiros diferentes em todos os meios de cultura. Também houve variação quanto às dimensões dos conídios dos isolados de C. cassiicola em todos os meios de cultura testados.
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Castillo, Teresa Alarcón, and 92-99245-8233. "Caracterização fisiológica, produção de biocompostos e biomassa em substrato agroflorestal amazônico de Pleurotus e Lentinus." Universidade Federal do Amazonas, 2009. http://tede.ufam.edu.br/handle/tede/6215.
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Previous issue date: 2009-02-21<br>FAPEAM - Fundação de Amparo à Pesquisa do Estado do Amazonas<br>This work presents the analysis of mycelial growth of Pleurotus ostreatus (Jacq .: Fr.) Kumm.
DPUA 1533, Pleurotus ostreatus (Jacq.: Fr.) Quél. cv. Florida DPUA 1534, Lentinus citrinus
Walleyn & Rammeloo DPUA 1535 and Neolentinus lepideus (Fr.) Redhead & Ginns DPUA
1536, in natural and complex media under different growing conditions to verify production
of biocompounds against fungi and bacteria, to evaluate vertical mycelial growth, to analyze
the production of proteases and biomass in agroindustrial residues selected by solid
fermentation. Antimicrobial activity of mushrooms against Candida albicans DPUA 1336,
Cryptococcus laurentii DPUA 1501, Aspergillus flavus DPUA1836, Escherichia coli DAUPE
224 and Mycobacterium smegmatis ATCC 607 was determined by the Gelose Block Method
and Minimum Inhibitory Concentration. For the production of Pleurotus and Lentinus
proteases by solid state fermentation, the cultivations were realized on 200 g of cupuaçu shell
+ rice husk (CC+CA); cupuaçu shell + bran rice (CC+FA), in flask of transparent glass,
humidity 60% and pH 6,0 and the sterilization of the substrates was accomplished at 121
°C/90 minutes for two consecutive days. Each flask was inoculated with 12 agar disks (10
mm in diameter) from stock culture and allowed to fermented for 15 days at 25 °C, without
light and mycelial growth was determined in centimeters every 24 hours. For determination of
proteolityc activity at 440 nm solution of azocasein 1% (p/v), pH 7,2 was used. L. citrinus
was the specie selected for biomass production. In this stage for the accomplishment of the
fermentation in the solid-state were prepared 5 kg plastic bags containing different
combinations and proportions of cupuaçu shell, rice husk and rice bran. Methods were carried
out to evaluate the formation, development of the primordia, total culture time, number of
production flows and analysis of centesimal composition of basidiomas of L. citrinus in
triplicate. The results demonstrate that the composition of culture media, the time of
cultivation, the light and the temperature influence significantly in the growth of Lentinus and
Pleurotus species, on culture media analyzed. In the experimental conditions, those
mushrooms express antimicrobial activity against most fungi and bacteria tested, however L.
citrinus and N. lepideus didn't express antimicrobial activity against Aspergillus flavus. The
mixture of cupuaçu shell and rice husk (1: 1) stimulated the vertical growth rate of L. citrinus
(13 cm), P. ostreatus (12.6 cm) and N. lepideus (12.3 cm). The best averages of the activity
and effect of the proteolytic enzyme extraction conditions were determined in the crude
extract of P. ostreatus (24.0 U / g) and L. citrinus (22.0 U / g) obtained in the NaCl 2 solution
% at 36 °C and with distilled water at 25 °C. In the biomass production, the best averages of
biological efficiency (61.0%), productivity (0.86%), production rate (32.0%) and protein
(13.44%) were observed in the formulations different agroforestry substrates that depending
on the concentration have demonstrated the viability of the process for the growth and
production of L. citrinus DPUA 1535.<br>Este trabalho apresenta a análise do crescimento de Jacq.: Fr.) Kumm.
DPUA 1533, Pleurotus ostreatus (Jacq.: Fr.) Quél. cv. Florida DPUA 1534, Lentinus citrinus
Walleyn & Rammeloo DPUA 1535 e Neolentinus lepideus (Fr.) Redhead & Ginns DPUA
1536, em meios natural e complexo sob diferentes condições de cultivo, para verificar a
produção de biocompostos contra fungos e bactérias, avaliar o crescimento micelial vertical,
analisar a produção das proteases e biomassa em resíduos agroindustriais selecionados por
fermentação sólida. A atividade antimicrobiana dos cogumelos frente à Candida albicans
DPUA 1336, Cryptococcus laurentii DPUA 1501, Aspergillus flavus DPUA 1836,
Escherichia coli DAUPE 224 e Mycobacterium smegmatis ATCC 607 foi determinada pelo
Método do Bloco de Gelose e pela Concentração Mínima Inibitória. Para a produção de
proteases de Pleurotus e Lentinus por fermentação no estado sólido, os cultivos foram
realizados em 200 g de casca de cupuaçu + casca de arroz (CC+CA); casca de cupuaçu +
farelo de arroz (CC+FA), em frasco de vidro transparente, umidade 60% e pH 6,0. A
esterilização dos substratos foi realizada a 121 °C/90 minutos/dois dias consecutivos. Em
cada frasco foram inoculados 12 discos de 10 mm de diâmetro e a fermentação foi conduzida
a 25 oC, sem luminosidade por 15 dias, determinando-se a velocidade de crescimento micelial
em centímetros, a cada 24 horas. Para determinação da atividade proteolítica a 440 nm foi
utilizado solução de azocaseina 1% (p/v), pH 7,2. L. citrinus foi a espécie selecionada para
produção de biomassa. Nesta etapa para a realização da fermentação no estado sólido foram
preparados sacos plásticos de 5 kg contendo diferentes combinações e proporções de casca de
cupuaçu, casca de arroz e farelo de arroz. Efetuaram-se processos para avaliar a formação,
desenvolvimento dos primórdios, tempo total de cultivo, número de fluxos de produção e
análises de composição centesimal de basidiomas de L. citrinus em triplicata. Os resultados
demonstraram que a composição dos meios de cultura, o tempo de cultivo, a luz e a
temperatura influenciam significativamente no crescimento das espécies de Lentinus e
Pleurotus, nos meios de cultura analisados. Nas condições experimentais, esses cogumelos
expressaram atividade antimicrobiana contra a maioria dos fungos e bactérias testados,
contudo L. citrinus e N. lepideus não expressaram atividade antimicrobiana frente Aspergillus
flavus. A mistura de casca de cupuaçu com casca de arroz (1:1) estimulou a velocidade de
crescimento vertical de L. citrinus (13 cm), P. ostreatus (12,6 cm) e N. lepideus (12,3 cm). As
melhores médias da atividade e efeito das condições de extração de enzimas proteolíticas
foram determinadas no extrato bruto de P. ostreatus (24,0 U/g) e L. citrinus (22,0 U/g),
obtidos na solução de NaCl 2%, a 36 °C e com água destilada, a 25 °C. Na produção de
biomassa, as melhores médias de eficiência biológica (61,0 %), produtividade (0,86 %), taxa
de produção (32,0 %) e proteína (13,44 %) foram observadas nas formulações realizadas com
os diferentes substratos agroflorestais que dependendo da concentração têm demonstrando a
viabilidade do processo para o crescimento e produção de L. citrinus DPUA 1535.
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Rosa, Janicéli [UNESP]. "Seleção de genótipos de guandu para resistência a Macrophomina phaseolina e esporulação do fungo." Universidade Estadual Paulista (UNESP), 2006. http://hdl.handle.net/11449/96898.
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rosa_j_me_jabo.pdf: 154567 bytes, checksum: 0d2897a42007e37a9a5f314b75f0f0f0 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)<br>Objetivou-se o ajuste de metodologia e seleção de genótipos de guandu para resistência a Macrophomina phaseolina a partir de material obtido pela Embrapa Pecuária Sudeste, e verificar o desenvolvimento micelial e esporulação do fungo em meios de cultura. O trabalho foi conduzido em casa de vegetação na UNESP/Jaboticabal no período de agosto de 2004 a dezembro de 2005. Para o ajuste de metodologia e seleção de genótipos resistentes ao fungo as sementes foram submetidas a escarificação com lixa d'água e inoculação artificial através do método de exposição das mesmas ao patógeno por diferentes períodos, que variaram de O a 72 horas. Foram avaliadas porcentagem de plantas sobreviventes e massa fresca. Já para o crescimento micelial e esporulação do fungo foi utilizado o método de sobreposição de discos de diferentes hospedeiros no meio de cultura. A escarificação das sementes contribuiu para a penetração do fungo nas mesmas o período de 24h de exposição das sementes ao fungo são suficientes para detectar diferenças no grau de resistência dos genótipos. Os genótipos mais resistentes são g167-97, g124-95, g27-94, g40-95, g154-95, g127-97 e g9m-97, e os mais suscetíveis são g48-95, g123-95, g8-95, g168-99 e g1m-95. A sobreposição de discos foliares de guandu em meio BDA e folha de papel de filtro em meio sojinha proporcionam um incremento na esporulação de M. phaseolina.<br>This work had the objective of determining the best schedule for artificial inoculation and select pigeon pea genotypes resistant to Macrophomina phaseolina in material obtained by Embrapa Pecuária Sudeste, and verify the mycelial growth and sporulation of the fungi in middle of culture. The work were carried in greenhouse at the UNESP/Jaboticabal, from August 2004 to December 2005. For the methodology and selection adjustment of resistant genotypes to the fungi the seeds were submitted scarified with water sandpaper and artificial inoculation the seeds were the contact method to fungi for different periods, which varied from O to 72 hours. They were evaluated percentage of surviving plants and fresh mass. For the mycelial growth and sporulation of the fungi was used the superposition of disks method of different hosts in the middle of culture. The scarified of the seeds contributed for penetration of the fungi at the seeds; the period of 24h of contact of the seeds to the fungi enough to detect differences in the resistance degree ofthe genotypes. The genotypes g167-97, g124-95, 927-94, g40-95, g154-95, g127-97 and g9m-97 were found to be the most resistant and most susceptible were g48-95, g123-95, g8-95, g168-99 and g1m-95. The treatment with superposition of the leaf disks of pigeon pea in BDA and disks of filter paper in middle of soybean extract were the treatments that provided better sporulation levei in the conditions of that experiment were half.
Book chapters on the topic "Mycelial growth and mycelial colonization"
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Orlowski, M. "Yeast/Mycelial Dimorphism." In Growth, Differentiation and Sexuality. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-11908-2_8.
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Pukkila, P. J. "Meiosis in Mycelial Fungi." In Growth, Differentiation and Sexuality. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-11908-2_15.
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Gooday, G. W. "Hormones in Mycelial Fungi." In Growth, Differentiation and Sexuality. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-11908-2_24.
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Ugalde, Unai, and Ana Belén Rodriguez-Urra. "9 Autoregulatory Signals in Mycelial Fungi." In Growth, Differentiation and Sexuality. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-25844-7_9.
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Glass, N. L., and M. A. Nelson. "Mating-Type Genes in Mycelial Ascomycetes." In Growth, Differentiation and Sexuality. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-11908-2_17.
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Giovannetti, Manuela. "Spore Germination and Pre-Symbiotic Mycelial Growth." In Arbuscular Mycorrhizas: Physiology and Function. Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-0776-3_3.
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Money, N. P. "Osmotic Adjustment and the Role of Turgor in Mycelial Fungi." In Growth, Differentiation and Sexuality. Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-11908-2_4.
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Gutiérrez-Rojas, M., R. Auria, J. C. Benet, and S. Revah. "A phenomenological model for solid state fermentation of fungal mycelial growth." In Advances in Solid State Fermentation. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-017-0661-2_11.
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Potapova, Tatiana. "Cell-to-Cell Communication in the Tip Growth of Mycelial Fungi." In Biocommunication of Fungi. Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-4264-2_7.
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Giovannetti, Manuela, Luciano Avio, and Cristiana Sbrana. "Fungal Spore Germination and Pre-symbiotic Mycelial Growth – Physiological and Genetic Aspects." In Arbuscular Mycorrhizas: Physiology and Function. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9489-6_1.
Full textConference papers on the topic "Mycelial growth and mycelial colonization"
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Keeratirawee, Kanchalar, Pornprapa Kongtragoul, and Sansanee Pantakan. "EFFECT OF ZINC OXIDE NANOPARTICLES AGAINST PHYTOPHTHORA SPP. CAUSING DURIAN DISEASES IN VITRO." In SGEM International Multidisciplinary Scientific GeoConference 24. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/6.1/s24.08.
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The efficiency of zinc oxide nanoparticles (ZnO-NPs) in inhibiting Phytophthora spp. growth in both durian fruits and stems is demonstrated. The size of ZnO-NPs used was in range of 25-50 nm in diameter. Diseased durian sample were collected from a durian orchard located in southern of Thailand. The pathogen of diseased durian was isolated for subsequent morphological identification. Three distinct strains of Phytophthora spp. (designated as PHY41, PHY45, PHY49) were successfully isolated and were found in colonies with rings and stellate pattern, ovoid, limoniform sporangium with semi-papillate or papillate, and formation chlamydospores. All isolates were exhibiting 100% pathogenicity on durian, as indicated by the disease incidence (DI). The concentration of ZnO-NPs played a crucial role in suppressing the growth of Phytophthora spp. in all isolates. Higher concentration of ZnO-NPs resulted in increased inhibition of growth. ZnO-NPs at the concentration of 2000 ?g/mL effectively suppressed the mycelial growth of all fungi isolates at 57.76-69.84%. Additionally, ZnO-NPs at the concentration of 500 ?g/mL completely inhibited the sporulation only in the PHY49 strains. Furthermore, ZnO-NPs concentration ranging from 1000-2000 ?g/mL resulted in completed inhibition in sporulation in all Phytophthora spp. isolates. The result demonstrated that ZnO-NPs at all concentrations effectively delayed the germination of Phytophthora spp.
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Nha, Phung Bac, Vo Ho Thuy Vy, Tran Thi Cam Hang, et al. "IDENTIFICATION AND INVESTIGATION ON MYCELIAL GROWTH OF SOME NATURAL Ganoderma." In THE 6TH VIETNAM NATIONAL MYCOLOGY CONFERENCE - 2024. Nhà xuất bản Khoa học tự nhiên và Công nghệ, 2024. https://doi.org/10.15625/vap.2025.0093.
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Kai, Priscila M., Fabrizzio A. A. de M.N. Soares, Ronaldo M. da Costa, Juliana Paula Felix, Jessica Maria I. de Jesus, and Marcos G. da Cunha. "Measurement by Images of Mycelial Growth Of Fungal Colonies On Petri Dishes." In 2019 IEEE Canadian Conference of Electrical and Computer Engineering (CCECE). IEEE, 2019. http://dx.doi.org/10.1109/ccece.2019.8861749.
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Никитина, З. К., И. К. Гордонова, and Э. М. Насибов. "MYCELIAL FUNGI GROWTH AND PRODUCTIVITY EVALUATION IN THE PROCESS OF DEEP CULTIVATION." In ОТ БИОХИМИИ РАСТЕНИЙ К БИОХИМИИ ЧЕЛОВЕКА. Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт лекарственных и ароматических растений", 2022. http://dx.doi.org/10.52101/9785870191041_112.
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Trang, Giap Hoang Thuy, Thach Thi Kim Ngoc, Dang Hoang Quyen, et al. "NEW RECORD OF Tylopilus glutinosus IN BINH CHAU - PHUOC BUU AND MYCELIAL GROWTH CHARACTERISTIC." In THE 6TH VIETNAM NATIONAL MYCOLOGY CONFERENCE - 2024. Nhà xuất bản Khoa học tự nhiên và Công nghệ, 2024. https://doi.org/10.15625/vap.2025.0085.
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Nurtjahja, Kiki, Albert Pasaribu, and Roslindawati. "The Effect of Basil Leaves Extract (Ocimum sanctum L.) on Mycelial Growth of Postharvest Fungi." In The International MIPAnet Conference on Science and Mathematics (IMC-SciMath). SCITEPRESS - Science and Technology Publications, 2019. http://dx.doi.org/10.5220/0010612700002775.
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Salifu, Emmanuel, Huda Clemens, and Edward Kavazanjian. "Evaluation of the Suitability of Engineered Growth of Fungal-Mycelial Networks for Reinforcement of Sands." In Soil Erosion Research Under a Changing Climate, January 8-13, 2023, Aguadilla, Puerto Rico, USA. American Society of Agricultural and Biological Engineers, 2023. http://dx.doi.org/10.13031/soil.2023045.
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Salifu, Emmanuel, Huda Clemens, and Edward Kavazanjian. "Evaluation of the Suitability of Engineered Growth of Fungal-Mycelial Networks for Reinforcement of Sands." In Soil Erosion Research Under a Changing Climate, January 8-13, 2023, Aguadilla, Puerto Rico, USA. American Society of Agricultural and Biological Engineers, 2023. http://dx.doi.org/10.13031/soil.23045.
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Perera, TVRC, K. Pakeerathan, and A. Nirosha. "ECO-FRIENDLY MANAGEMENT COMMON LAB CONTAMINANT Trichoderma spp IN OYSTER MUSHROOM PRODUCTION USING AGROBASED INDUSTRY’S BY-PRODUCTS." In The 5th International Conference on Climate Change 2021 – (ICCC 2021). The International Institute of Knowledge Management, 2021. http://dx.doi.org/10.17501/2513258x.2021.5105.
Full textAbstract:
An abundant supply of low-cost substrate and management of green mold disease-causing fungus Trichoderma are the major hurdles in successful mushroom production. This study aimed to identify the best Agro-based industry’s by-products as a substitute for oyster mushroom production (Pleurotus ostreatus) while managing fungal contaminants eco-friendly. Two sets of In-Vitro [containing 20% extracts, from agro-based industries, such as coffee waste powder, tea dust and Mahua oil cake] and In-Vivo experiments [four substrates such as paddy straw, wood sawdust, paddy husk and banana leaves were incorporated with coffee powder, tea dust and Mahua oil cake] were prepared separately. All the experiments were conducted using a complete randomized design with three replicates. The In-Vitro data [mycelial growth and sporulation of both fungi], In-Vivo data [mycelial mushroom run, pinhead formation and yield] were subjected to ANOVA and DMRT mean separation using SAS 9.1 statistical package at P <0.05. In-Vitro results showed that the Trichoderma mycelial growth was significantly minimum in Mahua (2.5 cM) and coffee (3.6 cM) in comparison to control, whereas, with decreasing concentration of coffee, tea, and Mahua extract P. ostreatus showed enhanced growth. Trichoderma sporulation had significantly affected coffee treatment, and even not sporulate in Mahua treated plants. The In-Vivo experiment proved that spawn run was consistent and significant among the treatments when mixed tea (20 days) and coffee (21 days), respectively, at P <0.05. Treatment wise coffee treated spawn bags took an average of 32.5 days, whereas, in tea-treated substrates, it was more than 36 days to form pinhead. Mahua treated trials showed poor spawn run in all substrates, longer days of pinhead formation, and lower yield. In contrast, the paddy straw + coffee treatment produced a significantly highest yield of 200.67g. When sawdust was the substrate, the addition of tea showed a significantly higher yield of 185.00g than coffee (145.00g). In conclusion, coffee and tea extracts have a significant effect on yield with paddy straw and sawdust while minimizing the growth of Trichoderma. Keywords: Pleurotus ostreatus, eco-friendly, plant extract, substrate, coffee, paddy straw
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Nguyen, Giang Van, Bich Thuy Thi Nguyen, Lan Huong Bui, et al. "Effects of nutrient sources and culture conditions on the mycelial growth of Ganoderma Lucidum strain Ga-TB." In INTERNATIONAL CONFERENCE “SUSTAINABLE DEVELOPMENT: VETERINARY MEDICINE, AGRICULTURE, ENGINEERING AND ECOLOGY” (VMAEE2022). AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0149073.
Full textReports on the topic "Mycelial growth and mycelial colonization"
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Lichter, Amnon, Gopi K. Podila, and Maria R. Davis. Identification of Genetic Determinants that Facilitate Development of B. cinerea at Low Temperature and its Postharvest Pathogenicity. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7592641.bard.
Full textAbstract:
Botrytis cinerea is the postharvest pathogen of many agricultural produce with table grapes, strawberries and tomatoes as major targets. The high efficiency with which B. cinerea causes disease on these produce during storage is attributed in part due to its exceptional ability to develop at very low temperature. Our major goal was to understand the genetic determinants which enable it to develop at low temperature. The specific research objectives were: 1. Identify expression pattern of genes in a coldenriched cDNA library. 2. Identify B. cinerea orthologs of cold-induced genes 3. Profile protein expression and secretion at low temperature on strawberry and grape supplemented media. 4. Test novel methods for the functional analysis of coldresponsive genes. Objective 1 was modified during the research because a microarray platform became available and it allowed us to probe the whole set of candidate genes according to the sequence of 2 strains of the fungus, BO5.10 and T4. The results of this experiment allowed us to validate some of our earlier observations which referred to genes which were the product of a SSH suppression-subtraction library. Before the microarray became available during 2008 we also analyzed the expression of 15 orthologs of cold-induced genes and some of these results were also validated by the microarray experiment. One of our goals was also to perform functional analysis of cold-induced genes. This goal was hampered for 3 years because current methodology for transformation with ‘protoplasts’ failed to deliver knockouts of bacteriordopsin-like (bR) gene which was our primary target for functional analysis. Consequently, we developed 2 alternative transformation platforms, one which involves an air-gun based technique and another which involves DNA injection into sclerotia. Both techniques show great promise and have been validated using different constructs. This contribution is likely to serve the scientific community in the near future. Using these technologies we generated gene knockout constructs of 2 genes and have tested there effect on survival of the fungus at low temperature. With reference to the bR genes our results show that it has a significant effect on mycelial growth of the B. cinerea and the mutants have retarded development at extreme conditions of ionic stress, osmotic stress and low temperature. Another gene of unknown function, HP1 is still under analysis. An ortholog of the yeast cold-induced gene, CCH1 which encodes a calcium tunnel and was shown to be cold-induced in B. cinerea was recently cloned and used to complement yeast mutants and rescue them from cold-sensitivity. One of the significant findings of the microarray study involves a T2 ribonuclease which was validated to be cold-induced by qPCR analysis. This and other genes will serve for future studies. In the frame of the study we also screened a population of 631 natural B. cinerea isolates for development at low temperature and have identified several strains with much higher and lower capacity to develop at low temperature. These strains are likely to be used in the future as candidates for further functional analysis. The major conclusions from the above research point to specific targets of cold-induced genes which are likely to play a role in cold tolerance. One of the most significant observations from the microarray study is that low temperature does not induce ‘general stress response in B. cinerea, which is in agreement to its exceptional capacity to develop at low temperature. Due to the tragic murder of the Co-PI Maria R. Davis and GopiPodila on Feb. 2010 it is impossible to deliver their contribution to the research. The information of the PI is that they failed to deliver objective 4 and none of the information which relates to objective 3 has been delivered to the PI before the murder or in a visit to U. Alabama during June, 2010. Therefore, this report is based solely on the IS data.