Relevant bibliographies by topics / Mycobacterial Mid-log Phase Cells

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Academic literature on the topic 'Mycobacterial Mid-log Phase Cells'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 September 2023

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Journal articles on the topic "Mycobacterial Mid-log Phase Cells"

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Vijay, Srinivasan, Nagaraja Mukkayyan, and Parthasarathi Ajitkumar. "Highly Deviated Asymmetric Division in Very Low Proportion of Mycobacterial Mid-log Phase Cells." Open Microbiology Journal 8, no. 1 (2014): 40–50. http://dx.doi.org/10.2174/1874285801408010040.

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In this study, we show that about 20% of the septatingMycobacterium smegmatisandMycobacterium xenopicells in the exponential phase populationdivideasymmetrically, with an unusually high deviation (17 ± 4%) in the division site from the median, to generate short cells and long cells, thereby generating population heterogeneity. This mode of division is very different from the symmetric division of themajority (about 80%) of the septating cells in theMycobacterium smegmatis,Mycobacterium marinum, andMycobacterium bovisBCG exponential phase population, with 5-10% deviation in the division site fr
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Pradhan, Atul, Nagaraja Mukkayyan, Kishor Jakkala, and Parthasarathi Ajitkumar. "Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium." Cells 10, no. 5 (2021): 1160. http://dx.doi.org/10.3390/cells10051160.

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Twenty to thirty percent of the septating mycobacterial cells of the mid-log phase population showed highly deviated asymmetric constriction during division (ACD), while the remaining underwent symmetric constriction during division (SCD). The ACD produced short-sized cells (SCs) and normal/long-sized cells (NCs) as the sister–daughter cells, but with significant differential susceptibility to antibiotic/oxidative/nitrite stress. Here we report that, at 0.2% glycerol, formulated in the Middlebrook 7H9 medium, a significantly high proportion of the cells were divided by SCD. When the glycerol c
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Dong, Xu, Suresh Bhamidi, Michael Scherman, Yi Xin, and Michael R. McNeil. "Development of a Quantitative Assay for Mycobacterial Endogenous Arabinase and Ensuing Studies of Arabinase Levels and Arabinan Metabolism in Mycobacterium smegmatis." Applied and Environmental Microbiology 72, no. 4 (2006): 2601–5. http://dx.doi.org/10.1128/aem.72.4.2601-2605.2006.

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ABSTRACT Treatment of either Mycobacterium tuberculosis or M. smegmatis with ethambutol results both in inhibition of arabinan synthesis and in copious loss of previously formed arabinan from the cell wall. The loss of arabinan has been shown to be due to the action of an endogenous arabinase. To better understand this phenomenon, a quantitative assay for endogenous arabinase was developed. Using the assay it was determined that various subcellular fractions of M. smegmatis showed significant amounts of endogenous arabinase activity. Surprisingly, treatment with ethambutol yielded only minor c
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Khan, Arshad, Arunmani Mani, Shen-An Hwang, et al. "A Bovine Adenovirus (BAdv) Expressing Mycobacterial Secreted Ag85B and a TLR-2 Stimulating Adjuvant Peptide Induces Robust Antigen Presentation In vitro and Improved Protection against Tuberculosis in Mice." Journal of Immunology 200, no. 1_Supplement (2018): 125.5. http://dx.doi.org/10.4049/jimmunol.200.supp.125.5.

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Abstract Human Adenovirus (HAdv) vectors expressing Mycobacterium tuberculosis (Mtb) derived antigens, have been tested as candidate boosters for BCG vaccine in animal models and Phase I human studies but failed to provide significant additional protection. Presumably, pre-existing immunity to HAdv among humans has been suggested to limit their efficacy as vectors. We earlier reported that novel bovine adenovirus (BAdv)-vectors induced robust T cell mediated immunity in mice and carried less risk of interference by human innate immunity. Thus, we evaluated BAdv vectors expressing Mtb derived a
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Udou, Takezo. "Extracellular hemolytic activity in rapidly growing mycobacteria." Canadian Journal of Microbiology 40, no. 4 (1994): 318–21. http://dx.doi.org/10.1139/m94-052.

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Little is known about virulence factors associated with rapidly growing mycobacteria. We evaluated 42 clinical isolates of Mycobacterium fortuitum and Mycobacterium chelonae and 4 reference strains of Mycobacterium smegmatis for the production of hemolysin (or hemolytic substance) as a possible contributor to the pathogenesis of disease caused by these organisms. All the strains tested possessed extracellular hemolytic activity that was stable after heating and proteinase treatment, and the active substance had a molecular weight less than 10 000. The activity accumulated in culture medium dur
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Falkinham, Joseph O. "Factors Influencing the Chlorine Susceptibility of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum." Applied and Environmental Microbiology 69, no. 9 (2003): 5685–89. http://dx.doi.org/10.1128/aem.69.9.5685-5689.2003.

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ABSTRACT The susceptibility of representative strains of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (the MAIS group) to chlorine was studied to identify factors related to culture conditions and growth phase that influenced susceptibility. M. avium and M. intracellulare strains were more resistant to chlorine than were strains of M. scrofulaceum. Transparent and unpigmented colony variants were more resistant to chlorine than were their isogenic opaque and pigmented variants (respectively). Depending on growth stage and growth rate, MAIS strains differed
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7

Sekar, Gomathi, R. Lakshmi, and N. Selvakumar. "The Assessment of Viability of M. Tuberculosis after Exposure to CPC Using Different Methods." International Journal of Bacteriology 2014 (January 28, 2014): 1–5. http://dx.doi.org/10.1155/2014/564109.

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Settings. National Institute for Research in Tuberculosis, Chennai. Objective. To assess the proportion of metabolically active cells of Mycobacterium tuberculosis after exposed to CPC using FDA-EB vital staining and viable counts on LJ medium. Mycolic acid content in M. tuberculosis after exposure to CPC was estimated using HPLC. Methods. Clinical isolates of M. tuberculosis and standard reference strain M. tuberculosis H37Rv were used for FDA-EB, viable count, and HPLC. Results. FDA/EB consistently stained 70–90% of log phase cells as green and the remaining cells as red-orange. After CPC tr
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8

Pham, Phuong, Yijun Shao, Michael M. Cox, and Myron F. Goodman. "Genomic landscape of single-stranded DNA gapped intermediates in Escherichia coli." Nucleic Acids Research 50, no. 2 (2021): 937–51. http://dx.doi.org/10.1093/nar/gkab1269.

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Abstract Single-stranded (ss) gapped regions in bacterial genomes (gDNA) are formed on W- and C-strands during replication, repair, and recombination. Using non-denaturing bisulfite treatment to convert C to U on ssDNA, combined with deep sequencing, we have mapped gDNA gap locations, sizes, and distributions in Escherichia coli for cells grown in mid-log phase in the presence and absence of UV irradiation, and in stationary phase cells. The fraction of ssDNA on gDNA is similar for W- and C-strands, ∼1.3% for log phase cells, ∼4.8% for irradiated log phase cells, and ∼8.5% for stationary phase
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Mallinson, D. J., and G. H. Coombs. "Biochemical characteristics of the metacyclic forms ofLeishmania majorandL. mexicana mexicana." Parasitology 98, no. 1 (1989): 7–15. http://dx.doi.org/10.1017/s0031182000059631.

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SummaryMetacyclic forms ofLeishmania majorand putative metacyclics ofL. mexicana mexicanawere found to occur in abundance in stationary phase cultures. These forms have been compared in several ways with promastigotes from mid-log phase cultures and, in the case ofL. m. mexicana, amastigotes. Metacyclics are smaller, contain less protein and appear more active than other promastigotes. Both forms of promastigote respire at a high rate in the absence of exogenous substrate. The free amino-acid contents of the various forms of the two species have been analysed. They differ in detail but alanine
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Sellmer, Jim, Dave Ellis, Brent McCown, Dennis McCabe, Dave Russell, and Brian Martinell. "ENHANCING CELL RECEPTIVITY TO GENE TRANSFER BY ELECTRIC DISCHARGE PARTICLE ACCELERATION." HortScience 25, no. 9 (1990): 1130e—1130. http://dx.doi.org/10.21273/hortsci.25.9.1130e.

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Successful recovery of plants transformed by direct gene transfer techniques relies on 3 factors: 1) a regenerable cell/tissue culture system, 2) a foreign DNA delivery system which can be fine tuned, and 3) a cell population receptive to the transfer and integration of foreign DNA into its genome. Cell receptivity to foreign DNA incorporation is being determined by bombarding poplar cells with gold particles coated with plasmid DNA containing a CaMV35s pro–meted β-glucuronidase chimeric gene construct. Histochemical GUS expression assays conducted after bombardment show that early log phase c
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Dissertations / Theses on the topic "Mycobacterial Mid-log Phase Cells"

1

Mukkayyan, Nagaraja. "Identification, Structural and Functional Characterisation of the Molecule that Induces Asymmetric Cell Division in Mycobacteria." Thesis, 2016. http://etd.iisc.ernet.in/handle/2005/2855.

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Phenotypic heterogeneity in terms of cell size, morphology, and metabolic status, which are believed to help the population survive under stress conditions, is known in mycobacterial populations. Such population heterogeneity had been observed in in vitro cultures, TB patients, and in animal models. Our laboratory had earlier shown that about 20-30% of the 15% septating cells of Mycobacterium smegmatis, Mycobacterium tuberculosis and Mycobacterium xenopi mid-log phase cultures divide by highly deviated asymmetric cell division (ACD), generating subpopulations of short cells and normal-sized/lo
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