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Dissertations / Theses on the topic 'Mycosen'

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Published: 4 June 2021
Last updated: 11 February 2022

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1

Levesque, Dominique. "Les mycoses des équides." Paris 5, 1989. http://www.theses.fr/1989PA05P127.

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2

Juddet, Josette. "Drogues et mycoses." Paris 5, 1989. http://www.theses.fr/1989PA05P098.

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3

Mercier, Nathalie. "Les dérivés de l'imidazole dans le traitement des mycoses : chimie, dosages, applications thérapeutiques." Bordeaux 2, 1997. http://www.theses.fr/1997BOR2P025.

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4

JEAN, MARIE-JOSEPHE. "Mycoses oesophagiennes." Saint-Etienne, 1988. http://www.theses.fr/1988STET6028.

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5

Baylet, Sandrine. "Techniques appliquées au diagnostic biologique des mycoses." Paris 5, 1999. http://www.theses.fr/1999PA05P205.

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6

SALMI, MARYSE. "Mycoses et sida." Lyon 1, 1990. http://www.theses.fr/1990LYO1M288.

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7

Vuillemard, Catherine. "Traitement des mycoses ophtalmiques, stratégie thérapeutique des kératomycoses et des endophtalmies à candida et aspergillus." Bordeaux 2, 1994. http://www.theses.fr/1994BOR2P074.

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8

Fourel, Didier. "Manifestations viscérales extraganglionnaires du mycosis fongoi͏̈de et de la maladie de Sezary." Bordeaux 2, 1989. http://www.theses.fr/1989BOR25123.

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9

Nguyen, Christiane. "Les mycoses : modes d'action et effets indésirables des antifongiques." Paris 5, 2001. http://www.theses.fr/2001PA05P039.

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10

Roche, Annie. "L'apport des nouveaux dérivés imidazoliques dans les mycoses." Bordeaux 2, 1995. http://www.theses.fr/1995BOR2P083.

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11

Bosc, Philippe, and PASCAL LEDAIN. "Contribution a l'etude des mycoses buccales." Rennes 1, 1987. http://www.theses.fr/1987REN12042.

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12

Grosicki, Rachel. "Les principales maladies d'origine hydrique." Paris 5, 1997. http://www.theses.fr/1997PA05P231.

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13

Fialaire, Anne. "Liposomes d'amphotéricine B : étude pharmacotoxicologique, mise en forme et contrôle d'une suspension liposomale." Paris 5, 1991. http://www.theses.fr/1991PA05P213.

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14

Shankland, Gillian Sheana. "Diagnostic, epidemiological and pathological aspects of opportunistic mycoses." Thesis, University of Glasgow, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293455.

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15

Camu, Karine. "Principales affections parasitaires et mycosiques des chiens et chats transmissibles à l'homme : conseil en officine." Bordeaux 2, 2000. http://www.theses.fr/2000BOR2P038.

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16

SENE, JEAN-MARC. "Les mycoses necrosantes des fosses nasales et des sinus." Nantes, 1994. http://www.theses.fr/1994NANT207M.

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17

Lam, M. F., and 林文輝. "Meta-analysis of different anti-fungal prophylactic treatments in neutropenic patients." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B3197062X.

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18

Vanetti, Annick Le Pape Patrice. "Mycoses superficielles cutanéo- muqueuses et enquête auprès de pharmaciens d'officine." [S.l.] : [s.n.], 2009. http://castore.univ-nantes.fr/castore/GetOAIRef?idDoc=57526.

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19

Catteau, Benoit. "Manifestations cutanees des zoonoses d'origine feline : a l'exception des mycoses." Lille 2, 1994. http://www.theses.fr/1994LIL2M078.

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20

Lam, M. F. "Meta-analysis of different anti-fungal prophylactic treatments in neutropenic patients." Click to view the E-thesis via HKUTO, 2002. http://sunzi.lib.hku.hk/hkuto/record/B3197062X.

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21

Hui, Wai-ting. "Serodiagnosis of Penicilliosis marneffei in HIV & non-HIV patients using a recombinant antigen Mp1p." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22029746.

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22

Delahousse, Géraldine Le Pape Patrice. "Les plantes à propriétés antifongiques." [S.l.] : [s.n.], 2003. http://theses.univ-nantes.fr/thesemed/PHdelahousse.pdf.

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23

Anezo, Julie Pagniez Fabrice. "La sporotrichose cas autochtones en Nouvelle-Calédonie /." [S.l.] : [s.n.], 2009. http://castore.univ-nantes.fr/castore/GetOAIRef?idDoc=53006.

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24

Ho, Yiu-cheung Timothy. "Biotyping in Penicillium marneffei." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22050310.

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25

OURGAUD, OLGA. "Les infections fongiques de la cornee : a propos de 4 observations." Clermont-Ferrand 1, 1991. http://www.theses.fr/1991CLF13816.

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26

OLLIER, ANNE. "Miscellanea mycologica ; dermatophytes isoles au chu de montpellier de 1990 a 1992 ; incidence du vih sur les mycoses." Montpellier 1, 1994. http://www.theses.fr/1994MON11137.

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27

VIGNEAUD, JABOT HELENE. "Evolution sur 23 ans (1965-1988) des epidermomycoses et de leurs agents en region lyonnaise." Lyon 1, 1989. http://www.theses.fr/1989LYO1M230.

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28

Seneau-Garreau, Claire Lacassin-Beller Flore. "Mycose profonde à Fusarium solani à propos d'un cas de hyalohyphomycose pulmonaire et médullaire chez un immunocompétent au Centre Hospitalier de Nouméa /." [S.l.] : [s.n.], 2004. http://theses.univ-nantes.fr/thesemed/MEDseneau.pdf.

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29

Fang, Zhuo. "Elucidation of the substrates of mycosin 3, an essential protease of Mycobacterium tuberculosis." Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/6752.

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Thesis (MScMedSc)--University of Stellenbosch, 2011.
ENGLISH ABSTRACT: Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), infects one third of the world’s population and kills 1.7 million people per year. The increasing prevalence of multi- and extensively drug resistant M. tuberculosis strains means that there is an urgent need to develop new anti-TB drugs. The genome of M. tuberculosis has five copies of the ESAT-6 gene clusters (ESX-1, -2, -3, -4 and -5), which are essential for the survival (ESX-3) and pathogenicity (ESX-1 and ESX-5) of the bacterium. The ESX clusters encode for proteins which form a novel secretion system which has been shown to secreted small T-cell antigens of the esx gene family, as well as other proteins such as the PE and PPE’s. The mycosins are a family of genes situated in the ESX clusters which encode for putative subtilisin-like serine proteases. These proteins are the most conserved proteins within the five clusters. Apart from their conserved protein sequence, mycosin-3 is also an essential protein specific to the mycobacteria, which makes it an attractive potential drug target. Identifying the substrate(s) of mycosin-3 could help to understand the function of this enzyme and discover novel inhibitors from which new drugs could be designed. We hypothesize that the secreted products of the ESX system could be potential substrates for the mycosins. Specifically, we hypothesize that PE5, PPE4, esxG and esxH (all found in ESX-3) might be the substrates for mycosin-3. Mycosin-3, PE5, PPE4, esxG and esxH were thus cloned, expressed and purified respectively. The four substrates were used for protease assays using mycosin-3 as the protease. The protease-substrate mixture were subsequently separated on 2-D SDS-PAGE gels to check whether there were any cleavage of the four substrates. Although all the target fusion proteins were cloned and expressed successfully, the protease assay results showed no cleavage for any of the four substrates. Possible explanations for the failure of cleavage were: (1) impure enzyme and substrate(s); (2) inappropriate buffer conditions; (3) the hypothesized substrates might not be the substrates of mycosin-3; and (4) incorrect folding or modification of the target fusion proteins might have taken place. Future research will aim to address these possible limitations in order to fully elucidate the function and substrate specificity of mycosin-3 and to use this information for the design of novel drugs against M. tuberculosis.
AFRIKAANSE OPSOMMING: Mycobacterium tuberculosis, die organisme wat tuberkulose (TB) veroorsaak, infekteer `n derde van die wêreld se bevolking en veroorsaak die dood van 1.7 miljoen mense per jaar. Die verhoogde voorkoms van multi- en ekstensiewe middelweerstandige stamme van M. tuberculosis beteken dat daar `n ernstige nodigheid is om nuwe anti-TB middels te ontwikkel. Die genoom van M. tuberculosis het vyf kopieë van die ESAT-6 geengroepe (ESX-1, -2, -3, -4 en -5), wat essensieel is vir die oorlewing (ESX-3) en patogenisiteit (ESX-1 and ESX-5) van die bakterium. Die ESX groepe enkodeer vir proteïene wat `n nuwe uitskeidingssisteem vorm wat bewys is om klein T-sel antigene van die esx geenfamilie, sowel as ander proteïene soos die PE en PPE proteïene uit te skei. Die mycosins is `n familie gene wat in die ESX geengroepe voorkom en wat waarskynlik enkodeer vir subtilisin-agtige serine proteases. Hierdie proteïene is die mees gekonserveerde proteïene in die vyf geengroepe. Mycosin-3 is `n essensiële protein wat spesifiek in die mikobakteriëe voorkom, sodat dit `n aantreklike teiken vir die ontwikkeling van middels is. Die identifisering van die substrate van mycosin-3 kan moontlik help om die funksie van die ensiem te verstaan en om nuwe inhibeerders vir die ensiem te ontdek, wat kan lei tot die onwikkeling van nuwe middels. Ons hipotese is dat die uitgeskeide proteïene van die ESX sisteem moontlik die substrate van die mycosin proteïene kan wees. Meer spesifiek, ons hipnotiseer dat die proteïene PE5, PPE4, esxG en esxH (wat almal in ESX-3 voorkom) die substrate vir mycosin-3 kan wees. Mycosin-3, PE5, PPE4, esxG en esxH is afsonderlik gekloneer, uitgedruk en gesuiwer. Die vier substrate is gebruik vir protease proewe met mycosin-3 as die protease. Die protease-substraat mengsel is hierna deur middel van 2-D SDS-PAGE geanaliseer om te kyk of daar enige kliewing van die vier substrate voorgekom het. Alhoewel al die teiken fusieproteïene suksesvol gekloneer, uitgedruk en gesuiwer is, het die protease proewe geen kliewing getoon vir enige van die vier potensiële substrate nie. Moontlike verklarings vir hierdie negatiewe resultaat is die volgende: (1) ensiem en substrate was moontlik onsuiwer; (2) bufferkondisies was moontlik nie korrek nie; (3) gehipotiseerde substrate mag moontlik nie substrate van mycosin-3 wees nie; en (4) nie-korrekte vouing of modifisering van die teiken proteïene kon moontlik plaasgevind het. Toekomstige navorsing sal daarop gemik wees om hierdie beperkinge aan te spreek om sodoende die funksie en substrate van mycosin-3 te kan ontdek en nuwe middels teen M. tuberculosis te ontwerp.
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30

Xia, Yuxian. "Fungal hydrolases in the haemolymph of mycosed insects and their roles in pathogenesis." Thesis, University of Bath, 2001. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341170.

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31

Bismuth, Anne. "Mycoses et parasitoses du tégument et des branchies chez les poissons d'aquarium dulçaquicoles." Paris 5, 1997. http://www.theses.fr/1997PA05P196.

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32

Nivoix, Yasmine. "Infections fongiques invasives : épidémiologie et optimisation thérapeutique." Strasbourg, 2009. http://www.theses.fr/2009STRA2006.

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33

Scarisbrick, Julia Jane. "Molecular genetics of mycosis fungoides and Sezary syndrome." Thesis, King's College London (University of London), 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271821.

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34

CLAUDE, PIGNON CATHERINE. "Les mycoses oesophagiennes : considerations etiologiques, cliniques, diagnostiques et therapeutiques : a propos d'une etude retrospective de 87 observations." Reims, 1990. http://www.theses.fr/1990REIMM007.

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35

Houn, Sarath. "Les anevrysmes mycotiques vrais : a propos d'un cas." Aix-Marseille 2, 1988. http://www.theses.fr/1988AIX20416.

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36

Dumaine, Pierre. "Les maladies parasitaires en inde." Aix-Marseille 2, 1990. http://www.theses.fr/1990AIX20057.

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37

Chan, Hui Min Ravis William R. "Pharmacokinetics of Voriconazole in horses and alpacas." Auburn, Ala, 2008. http://repo.lib.auburn.edu/EtdRoot/2008/SPRING/Pharmacal_Sciences/Dissertation/Chan_Hui_20.pdf.

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38

何耀祥 and Yiu-cheung Timothy Ho. "Biotyping in Penicillium marneffei." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31969732.

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39

顔鴻儀 and Hung-yee Ngan. "Molecular diagnosis of penicilliosis marneffei." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970035.

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40

Bergeau, Caroline Ballereau Françoise. "Place du voriconazole et de la caspofungine dans le traitement des infections fongiques invasives au CHU de Nantes." [S.l.] : [s.n.], 2005. http://theses.univ-nantes.fr/thesemed/PHbergeau.pdf.

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41

Ngan, Hung-yee. "Molecular diagnosis of penicilliosis marneffei." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23595978.

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42

David, Dominique. "Une alternariose cutanée d'évolution prolongée chez un patient non immuno-déprimé." Bordeaux 2, 1990. http://www.theses.fr/1990BOR25267.

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43

Puygauthier-Toubas, Dominique. "Contribution à l'étude des isotypes spécifique et des antigènes fonctionnels dans les parasitoses et mycoses." Reims, 1990. http://www.theses.fr/1990REIMM201.

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44

CAZAUX, RASPAUD DOMINIQUE. "Etude d'une nouvelle technique immunoelectrophoretique appliquee au diagnostic des parasitoses, des mycoses et des pneumopathies immunologiques." Toulouse 3, 1988. http://www.theses.fr/1988TOU31315.

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45

林瑋熙 and Wai-hei Lam. "X-ray crystallographic studies of two virulence factors from two fungal pathogens, P. marneffei and C. neoformans." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/193057.

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Mycoses refer to infections caused by different fungal infections. Some mycoses can be defeated by the hosts themselves attributed to the functional immune systems before severe symptoms appear. However, in immune-compromised patients, including those suffering from AIDS or receiving chemotherapies, those mycoses become lethal. They are called opportunistic systemic mycoses. Among them, two types of the most deadly mycoses, especially for AIDS patients in Southeast Asia, are cryptococcsis and penicillosis, caused by Cryptococcus neoformans (C. neoformans) and Penicillium marneffei (P. marneffei), respectively. Both of them have their own virulence factors to enhance their pathogenicities and survival in hosts. Active research to explore these virulence factors in these two funguses is ongoing. Two proteins from these two pathogens were found to be putative novel virulence factors, MP1p from P. marneffei, and CPL1 from C. neoformans. Collaborators have successfully found that MP1p strongly bound arachidonic acids (AA), the sole precursor of paracrine signaling molecules essential to the onset of inflammatory responses, by various functional studies. This led to the hypothesis that MP1p might be able to suppress inflammatory responses and subsequent immune responses via removal of AA from macrophages engulfed P. marneffei. In this work, X-ray crystal structures of MP1p’s ligand-binding domain 2 (LBD2) from P. marneffei (strain MP1) overexpressed in E. coli, in complex with one and two AA molecules, were successfully solved by molecular replacement method. The resolutions were up to 1.45 Å and 1.50 Å respectively. These structures revealed detailed interactions between MP1p-LBD2 and AA.A possible ligands-dependent dimer-monomer transition in LBD2 was also revealed by both analytical size exclusion chromatography and crystallography. Full length CPL1 overexpressed in yeast was also successfully purified and crystallized. A 3.0 Å native dataset was collected. Heavy atoms derivatives of the crystals would be produced in order to solve the structure via experimental phasing methods. The structural determination of these virulence factors may provide molecular bases at atomic resolution for the developments of drugs targeting MP1p and CPL1 by structure-based drug design to treat, particularly, penicillosis and cryptococcsis in immune-compromised patients.
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46

Talarmin, Jean-Philippe Apaire-Marchais Véronique. "Développement d'une méthode de diagnostic moléculaire (PCR-TGGE) pour l'identification des champignons associés à la mucoviscidose." [S.l.] : [s.n.], 2007. http://castore.univ-nantes.fr/castore/GetOAIRef?idDoc=25126.

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47

Cognée, Grégory Le Pape Patrice. "L'anidulafungine nouvel antifongique de la classe des échinocandines /." [S.l.] : [s.n.], 2009. http://castore.univ-nantes.fr/castore/GetOAIRef?idDoc=57651.

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48

Leung, Sau-man Sally. "Serodiagnostic utility of an ELISA assay based on a recombinant antigen MP1 of penicillium marneffei." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2337309X.

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49

Wahbi, Youssef. "Synthèse et évaluation de l'activité antifongique de nouveaux analogues du miconazole." Toulouse 3, 1995. http://www.theses.fr/1995TOU30048.

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Les derives azoles constituent une famille importante d'antifongiques utilises en therapeutique humaine. Dans ce travail une quarantaine d'analogues du miconazole ont ete prepares par remplacement de l'un ou de l'autre des deux noyaux aromatiques presents dans cette molecule et ce par differents autres motifs aromatiques ou par des chaines aliphatiques saturees ; le noyau imidazole a egalement ete remplace par le noyau 1,2,4-triazole. Une etude spectrometrique de resonance magnetique nucleaire du proton a permis de supposer l'existence de structures figees pour les produits obtenus et pour les alcools intermediaires correspondants. Les parametres de lipophilie ont ete determines par chromatographie sur couche mince et par chromatographie liquide haute performance. L'activite antifongique evaluee in vitro sur differentes souches du genre candida a ete reliee a la structure et aux parametres de lipophilie. Le remplacement de l'un ou de l'autre des deux noyaux aromatiques n'a pas le meme effet sur l'activite, bien que les parametres de lipophilie restent voisins. Par contre, l'introduction d'une chaine aliphatique saturee montre une relation etroite entre lipophilie et activite ; certains composes ayant des chaines a 5 ou 6 carbones ont des activites et des parametres de lipophilie analogues a ceux du modele
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50

Martin-Monier, Isabelle. "L'itraconazole." Paris 5, 1994. http://www.theses.fr/1994PA05P007.

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