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Journal articles on the topic 'N-Alkanes Biodegradation'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Wang, Minzhen, Mingzhu Ding, and Yingjin Yuan. "Bioengineering for the Microbial Degradation of Petroleum Hydrocarbon Contaminants." Bioengineering 10, no. 3 (2023): 347. http://dx.doi.org/10.3390/bioengineering10030347.

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Petroleum hydrocarbons are relatively recalcitrant compounds, and as contaminants, they are one of the most serious environmental problems. n-Alkanes are important constituents of petroleum hydrocarbons. Advances in synthetic biology and metabolic engineering strategies have made n-alkane biodegradation more designable and maneuverable for solving environmental pollution problems. In the microbial degradation of n-alkanes, more and more degradation pathways, related genes, microbes, and alkane hydroxylases have been discovered, which provide a theoretical basis for the further construction of

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2

Costa, Carlos, Anais Santos, and Milena A. Vega. "Kinetics of Arab Light Crude Oil Degradation by Pseudomonas and Bacillus Strains." Water 14, no. 23 (2022): 3802. http://dx.doi.org/10.3390/w14233802.

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The biodegradation of crude oil is a consequence of the presence of a specific enzymatic system in the microorganisms selected: the alkane hydroxylase (AlkH). The enzymatic biodegradation has been described since 1994, when the enzyme was first isolated from P. putida (formerly P. oleovorans), but the kinetics of microbial degradation has been weakly considered. We studied and described in this work the kinetics of Arab Light biodegradation, a light crude oil used for gasoline production (46.4% C7–C12 n-alkanes), using two oleophilic strains (Bacillus licheniformis and Pseudomonas putida). Alk

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3

Whyte, Lyle G., Jalal Hawari, Edward Zhou, Luc Bourbonnière, William E. Inniss, and Charles W. Greer. "Biodegradation of Variable-Chain-Length Alkanes at Low Temperatures by a Psychrotrophic Rhodococcussp." Applied and Environmental Microbiology 64, no. 7 (1998): 2578–84. http://dx.doi.org/10.1128/aem.64.7.2578-2584.1998.

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ABSTRACT The psychrotroph Rhodococcus sp. strain Q15 was examined for its ability to degrade individual n-alkanes and diesel fuel at low temperatures, and its alkane catabolic pathway was investigated by biochemical and genetic techniques. At 0 and 5°C, Q15 mineralized the short-chain alkanes dodecane and hexadecane to a greater extent than that observed for the long-chain alkanes octacosane and dotriacontane. Q15 utilized a broad range of aliphatics (C10 to C21 alkanes, branched alkanes, and a substituted cyclohexane) present in diesel fuel at 5°C. Mineralization of hexadecane at 5°C was sign

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4

Ali, Ramadan, Tatjana Solevic-Knudsen, Malisa Antic, et al. "Degradability of n-alkanes during ex situ natural bioremediation of soil contaminated by heavy residual fuel oil (mazut)." Journal of the Serbian Chemical Society 78, no. 7 (2013): 1035–43. http://dx.doi.org/10.2298/jsc120829106a.

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It is well known that during biodegradation of oil in natural geological conditions, or oil pollutants in the environment, a degradation of hydrocarbons occurs according to the well defined sequence. For example, the major changes during the degradation process of n-alkanes occur in the second, slight and third, moderate level (on the biodegradation scale from 1 to 10). According to previous research, in the fourth, heavy level, when intensive changes of phenanthrene and its methyl isomers begin, n-alkanes have already been completely removed. In this paper, the ex situ natural bioremediation

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5

Xia, Xuefeng, Wenjuan Jia, Kai Wang, and Aizhong Ding. "Seasonal Distribution of Microbial Community and n-Alkane Functional Genes in Diesel-Contaminated Groundwater: Influence of Water Table Fluctuation." Water 17, no. 11 (2025): 1710. https://doi.org/10.3390/w17111710.

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Water table fluctuation alters environment properties and n-alkane transformation, leading to shifts in the groundwater microbial community and functions. A diesel-contaminated aquifer column experiment of seasonal water table fluctuation was designed to explore the mechanisms. Temporal changes in geochemical parameters, n-alkane concentration, bacterial community and functional gene composition were investigated. The results showed that water table fluctuation accelerated the depletion of the diesel n-alkane leakage point. Owing to the variations in the water table, the electron donors (disso

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6

Al-Kanany‎, Fadhil N., Muntasser M. Al-Maliki‎, Rasha M. Othman‎, and Raghad S. Jaafar‎. "The importance of Pseudomonas aeruginosa plasmids for the n-alkanes biodegradation ‎ability." Mesopotamian Journal of Marine Sciences 38, no. 1 (2023): 47–54. http://dx.doi.org/10.58629/mjms.v38i1.327.

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The study was conducted to evaluate the importance of Pseudomonas aeruginosa plasmids in their efficiency for n-alkanes biodegradation. Pseudomonas aeruginosa was isolated and characterized using biochemical and genetic tests, then acridine orange was used as a chemical plasmid curing agent, the loss of plasmids was confirmed by profiling using agarose gel electrophoresis comparing with the wild type, the two bands appear in the gel which located between 3Kbp to 4Kbp refer to the two plasmids harbouring by wild type. The biodegradation efficiency of wild and plasmid cured bacteria was 85% and

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7

Thontowi, Ahmad, Elvi Yetti, and Yopi Yopi. "Medium Chain and Long Chain Alkanes Hydroxylase Producing Whole Cell Biocatalyst From Marine Bacteria." ANNALES BOGORIENSES 22, no. 1 (2018): 12. http://dx.doi.org/10.14203/ab.v22i1.329.

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Alkanes are major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. The are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of Pristane-C19H40). The study showed twenty-nine isolates hav

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8

Thontowi, Ahmad, Elvi Yetti, and Yopi Yopi. "Medium Chain and Long Chain Alkanes Hydroxylase Producing Whole Cell Biocatalyst From Marine Bacteria." ANNALES BOGORIENSES 22, no. 1 (2018): 12. http://dx.doi.org/10.14203/ann.bogor.2018.v22.n1.12-19.

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Alkanes are major component of crude oil that could be hydrolyzed by the enzyme of alkane hydroxylase. The are three types of alkane hydroxylase based on the chain length of alkane such as short-chain length/SCL (C2-C4), medium-chain length/MCL (C5-C17), and long-chain length/LCL (C>18). The aims of this study were to characterize and identify alkanes-degrading bacteria from these bacteria. The 30 strains from marine were grown on MCL (Pentane-C5H12, Decane-C10H22, and Pentadecane-C15H32) and LCL (n-Paraffin-C12H19C17 and branch of Pristane-C19H40). The study showed twenty-nine isolates hav

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9

Pedentchouk, Nikolai, Barry Bennett та Steve Larter. "δ13C and δD Values of n-Alkanes from In-Reservoir Biodegraded Oils: Implications for Understanding the Mechanisms of Biodegradation and for Petroleum Exploration". Geosciences 11, № 9 (2021): 365. http://dx.doi.org/10.3390/geosciences11090365.

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This study investigates the magnitude and direction of stable C and H isotope shifts of n-C15–30 alkanes from biodegraded oils sourced from Type II (Oil suite S) and Type II/III (Oil suite H) kerogens. Compound-specific isotope data show a 2.0‰ 13C-enrichment and no D-enrichment of n-alkanes in the most biodegraded oil from sample suite S. Similarly, there is a 1.5–2.5‰ 13C-enrichment and no D-enrichment in Oil suite H. Overall, there is a <2.5‰ δ13C and <20‰ δD variability among individual n-alkanes in the whole sequence of biodegradation. N-alkanes from the least biodegraded Oil H samp

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10

Solevic, Tatjana, Milan Novakovic, Mila Ilic, Malisa Antic, Miroslav Vrvic, and Branimir Jovancicevic. "Investigation of the bioremediation potential of aerobic zymogenous microorganisms in soil for crude oil biodegradation." Journal of the Serbian Chemical Society 76, no. 3 (2011): 425–38. http://dx.doi.org/10.2298/jsc100531033s.

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The bioremediation potential of the aerobic zymogenous microorganisms in soil (Danube alluvium, Pancevo, Serbia) for crude oil biodegradation was investigated. A mixture of paraffinic types of oils was used as the substrate. The laboratory experiment of the simulated oil biodegradation lasted 15, 30, 45, 60 and 75 days. In parallel, an experiment with a control sample was conducted. Extracts were isolated from the samples with chloroform in a separation funnel. From these extracts, the hydrocarbons were isolated by column chromatography and analyzed by gas chromatography-mass spectrometry (GC-

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11

Lindley, Nicholas D. "Bioconversion and biodegradation of aliphatic hydrocarbons." Canadian Journal of Botany 73, S1 (1995): 1034–42. http://dx.doi.org/10.1139/b95-354.

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Aliphatic hydrocarbons represent a substantial energy reserve but also constitute a useful feedstock for the biotechnological production of various alkane-derived commodity chemicals. In addition, the biodegradation of aliphatic hydrocarbons continues to pose problems for fuel stocks with associated corrosion and eventual motor filter blocking. A relatively high number of yeasts and filamentous fungi have been described that degrade n-alkanes, but relatively few have received thorough investigation. Early work exploiting hydrocarbons as a potential substrate for unicellular protein production,

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12

Smith, Christy A., Kirk T. O'Reilly, and Michael R. Hyman. "Cometabolism of Methyl tertiary Butyl Ether and Gaseous n-Alkanes by Pseudomonas mendocina KR-1 Grown on C5 to C8n-Alkanes." Applied and Environmental Microbiology 69, no. 12 (2003): 7385–94. http://dx.doi.org/10.1128/aem.69.12.7385-7394.2003.

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ABSTRACT Pseudomonas mendocina KR-1 grew well on toluene, n-alkanes (C5 to C8), and 1° alcohols (C2 to C8) but not on other aromatics, gaseous n-alkanes (C1 to C4), isoalkanes (C4 to C6), 2° alcohols (C3 to C8), methyl tertiary butyl ether (MTBE), or tertiary butyl alcohol (TBA). Cells grown under carbon-limited conditions on n-alkanes in the presence of MTBE (42μ mol) oxidized up to 94% of the added MTBE to TBA. Less than 3% of the added MTBE was oxidized to TBA when cells were grown on either 1° alcohols, toluene, or dextrose in the presence of MTBE. Concentrated n-pentane-grown cells oxidiz

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13

Liu, Zhe, Yang Zhang, Xiumin Li, et al. "The Application of Biochar Derived from Rice Husk Enhanced the Bioremediation of Petroleum-Contaminated Soil in Semi-Arid Areas." Agronomy 14, no. 9 (2024): 2015. http://dx.doi.org/10.3390/agronomy14092015.

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With the continuous expansion of petroleum exploitation, a large amount of petroleum hydrocarbons has leaked into the Earth’s soil, which seriously threatens ecological health. Hence, a series of experiments were conducted to evaluate the performance of natural attenuation (NA), rice husk (RH), and biochar derived from rice husk (RHBC) in enhancing the bioremediation of petroleum-contaminated soil. When the biodegradation time reached 100 d, the total petroleum hydrocarbons (TPHs) biodegradation amounts of NA, RH, and RHBC were 3688.2, 4557.9, and 5913.3 mg/kg, which was equal to the biodegrad

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14

Zetra, Yulfi, R. Y. Perry Burhan, Maulida Fitriyyah, and Zjahra Vianita Nugrahaeni. "Aliphatic Biomarker Signatures of Crude Oil from Tarakan Subbasin, Tarakan Basin, North Kalimantan, Indonesia." Indonesian Journal on Geoscience 10, no. 2 (2023): 139–50. http://dx.doi.org/10.17014/ijog.10.2.139-150.

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Organic geochemical studies of crude oil from Pamusian Field, Tarakan Subbasin, Tarakan Basin, North Kalimantan, have been done. Biomarker aliphatic hydrocarbon fractions were identified using a combined gas chromatography-mass spectrometer (GC - MS). Identified biomarkers consist of n-alkane groups, isoprenoids, bicyclic sesquiterpenoids, and pentacyclic triterpenoids. The most abundant aliphatic hydrocarbon biomarker is pristane followed by n-C19. The existence of n-alkanes shows a homologous (n-C16- n-C30) with a unimodal distribution type. The abundance of n-C19 is higher than other n-alka

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15

Peng, Wanli, Xiuli Wang, Qinchen Liu, et al. "The GntR/VanR transcription regulator AlkR represses AlkB2 monooxygenase expression and regulates n‐alkane degradation in Pseudomonas aeruginosa SJTD‐1." mLife 4, no. 2 (2025): 126–42. https://doi.org/10.1002/mlf2.70004.

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AbstractTransmembrane alkane monooxygenase (AlkB)‐type monooxygenases, especially AlkB2 monooxygenases, are crucial for aerobic degradation of the medium‐to‐long‐chain n‐alkanes in hydrocarbon‐utilizing microorganisms. In this study, we identified a GntR/VanR transcription regulator AlkR of Pseudomonas aeruginosa SJTD‐1 involved in the negative regulation of AlkB2 and deciphered its nature of DNA binding and ligand release. The deletion of alkR enhanced the transcription levels of the alkB2 gene and the utilization efficiency of the medium‐to‐long‐chain n‐alkanes by strain SJTD‐1. The dimer of

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Hamza, Khalifa, Ali Ahmed Abdussalam, Abusaediyah Ayiman, Yousuf Aejeeliyah, and Grimida Salheen. "GAS CHROMATOGRAPHY - MASS SPECTROMETRY (GC-MS) IN ORGANIC GEOCHEMICAL INVESTIGATION OF CRUDE OILS FROM KIKINDA AND VELEBIT FIELDS IN SERBIA." International Journal of Research - Granthaalayah 5, no. 6 (2017): 550–60. https://doi.org/10.5281/zenodo.823622.

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In this work two crude oil samples were investigated to present the difference between biodegraded (Velebit) and non-degraded (Kikinda) oil. Two samples are from the Velebit oil–gas field and the Kikinda oil-gas field. These are two of the largest oil and gas deposits in the Serbian part of the Pannonian Basin. In the experimental part of this work, two samples of crude oil were separated by column chromatography. Saturated hydrocarbons were analyzed by gas chromatography-mass spectrometry instruments. Based on the abundance and distribution of biomarkers, it could be conclude that the distrib

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17

Ueno, Ryohei, Shun Wada, and Naoto Urano. "Repeated batch cultivation of the hydrocarbon-degrading, micro-algal strain Prototheca zopfii RND16 immobilized in polyurethane foam." Canadian Journal of Microbiology 54, no. 1 (2008): 66–70. http://dx.doi.org/10.1139/w07-112.

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This study reports on the stability of the cells of a heterotrophic green micro-algal strain Prototheca zopfii RND16 immobilized in polyurethane foam (PUF) cubes during degradation of mixed hydrocarbon substrate, which was composed of n-alkanes and polycyclic aromatic hydrocarbons (PAHs), in 5 successive cycles of repeated batch cultivation at 30 °C. Both RND16 cells and mixed hydrocarbon substrate components had been entrapped in PUF cubes through cultivation. PUF-immobilized RND16 degraded n-alkanes almost completely, whereas the strain hardly degraded PAHs in PUFs, rather they accumulated i

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18

Sun, Yanyu, Hui Wang, Junde Li, Bin Wang, Cancan Qi, and Xiaoke Hu. "Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities." Journal of Oceanology and Limnology 36, no. 4 (2018): 1294–303. http://dx.doi.org/10.1007/s00343-018-6310-y.

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Hamamura, Natsuko, Sarah H. Olson, David M. Ward, and William P. Inskeep. "Microbial Population Dynamics Associated with Crude-Oil Biodegradation in Diverse Soils." Applied and Environmental Microbiology 72, no. 9 (2006): 6316–24. http://dx.doi.org/10.1128/aem.01015-06.

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ABSTRACT Soil bacterial population dynamics were examined in several crude-oil-contaminated soils to identify those organisms associated with alkane degradation and to assess patterns in microbial response across disparate soils. Seven soil types obtained from six geographically distinct areas of the United States (Arizona, Oregon, Indiana, Virginia, Oklahoma, and Montana) were used in controlled contamination experiments containing 2% (wt/wt) crude oil spiked with [1-14C]hexadecane. Microbial populations present during hydrocarbon degradation were analyzed using both 16S rRNA gene sequence an

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Yakabe, Yoshikuni, Ken-ichi Kusumoto, Shin-ich Takeuchi, and Etsuko Hasegawa. "INVESTIGATION OF THE NATURAL CLEANUP MECHANISM OF SPILLED OIL IN SEA." International Oil Spill Conference Proceedings 1997, no. 1 (1997): 669–74. http://dx.doi.org/10.7901/2169-3358-1997-1-669.

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ABSTRACT A field survey of the Wakaura Bay oil spill and a laboratory biodegradability test of weathered crude oil were carried out to understand the natural cleanup mechanism of oil spilled in the sea. The petroleum hydrocarbons were eliminated from the intertidal sediment to nearly the same level as the uncontaminated sediment within 1 year. The elimination rates of both n-alkanes and branched alkanes were faster than those of the aromatic compounds. More than 20 times higher concentrations of petroleum hydrocarbons than those in the uncontaminated organism were observed in the bivalve Septi

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Disnar, J. R., and Y. Héroux. "Dégradation et lessivage des hydrocarbures de la formation ordovicienne de Thumb Mountain encaissant le gîte Zn–Pb de Polaris (Territoires du Nord-Ouest, Canada)." Canadian Journal of Earth Sciences 32, no. 7 (1995): 1017–34. http://dx.doi.org/10.1139/e95-084.

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Carbonate rocks of the Ordovician Thumb Mountain Formation, host to the Zn–Pb Polaris deposit, contain hydrocarbons that show types of alteration classically attributed to biodegradation and water washing. The hydrocarbons of the upper part of this formation and of the overlying Irene Bay Formation indicate alterations due to water washing only. The hydrocarbons of the impermeable shales of the overlying Cape Phillips Formation display indices of only higher thermal maturity than the underlying units. Contrary to classical concepts of hydrocarbon biodegradation, n-alkanes, cyclohexylalkanes, e

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22

Dai, Xiaoli, Jing Lv, Wenxia Wei, and Shaohui Guo. "Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil." Processes 9, no. 11 (2021): 2025. http://dx.doi.org/10.3390/pr9112025.

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High-efficiency bioremediation technology for heavy oil pollution has been a popular research topic in recent years. Laccase is very promising for the remediation of heavy oil pollution because it can not only convert bio-refractory hydrocarbons into less toxic or completely harmless compounds, but also accelerate the biodegradation efficiency of heavy oil. However, there are few reports on the use of laccase to enhance the biodegradation of heavy oil. In this study, we investigated the effect of laccase on the bacterial consortia degradation of heavy oil. The degradation efficiencies of bacte

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23

Geng, Xiaolong, and Michel C. Boufadel. "Modeling Biodegradation of Subsurface Oil in Sand Beaches Polluted with Oil." International Oil Spill Conference Proceedings 2014, no. 1 (2014): 1113–25. http://dx.doi.org/10.7901/2169-3358-2014.1.1113.

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ABSTRACT In April 2010, the explosion of the Deepwater Horizon (DWH) drilling platform led to the release of nearly 4.9 million barrels of crude oil into the Gulf of Mexico. The oil was brought to the supratidal zone of beaches (landward of the high tide line) by waves during storms, and was buried during subsequent storms. The objective of this paper is to investigate the biodegradation of subsurface oil in a tidally influenced sand beach located at Bon Secour National Wildlife Refuge and polluted by the DWH oil spill. Two transects were installed perpendicular to the shoreline within the sup

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24

Li, Shanshan, Dan Wang, Dan Du, Keke Qian, and Wei Yan. "Characterization of co-metabolic biodegradation of methyl tert-butyl ether by a Acinetobacter sp. strain." RSC Advances 9, no. 67 (2019): 38962–72. http://dx.doi.org/10.1039/c9ra09507a.

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Acinetobacter sp. SL3 could co-metabolically degrade MTBE when grown on n-alkanes. An extremely low TBA accumulation were achieved on n-octane. The fed-batch reactor degradation revealed continuous MTBE degradation capacity by Acinetobacter sp. SL3.

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Khalifa, Hamza, Abdussalam Ali Ahmed, Ayiman Abusaediyah, Aejeeliyah Yousuf, and Salheen Grimida. "GAS CHROMATOGRAPHY - MASS SPECTROMETRY (GC-MS) IN ORGANIC GEOCHEMICAL INVESTIGATION OF CRUDE OILS FROM KIKINDA AND VELEBIT FIELDS IN SERBIA." International Journal of Research -GRANTHAALAYAH 5, no. 6 (2017): 550–60. http://dx.doi.org/10.29121/granthaalayah.v5.i6.2017.2073.

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In this work two crude oil samples were investigated to present the difference between biodegraded (Velebit) and non-degraded (Kikinda) oil. Two samples are from the Velebit oil–gas field and the Kikinda oil-gas field. These are two of the largest oil and gas deposits in the Serbian part of the Pannonian Basin. In the experimental part of this work, two samples of crude oil were separated by column chromatography. Saturated hydrocarbons were analyzed by gas chromatography-mass spectrometry instruments. Based on the abundance and distribution of biomarkers, it could be conclude that the distrib

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26

Stevanovic, Jelena, Anton Rakitin, Ivan Kojic, Nikola Vukovic, and Ksenija Stojanovic. "Significance of infrared spectroscopic branching factor for investigation of structural characteristics of alkanes, geochemical properties and viscosity of oils." Journal of the Serbian Chemical Society 87, no. 1 (2022): 41–55. http://dx.doi.org/10.2298/jsc210830091s.

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A detailed investigation of significance of the infrared (IR) spectroscopic branching factor (??2/??3; the ratio of methylene and methyl group peak heights at 2917?2921 and 2951?2954 cm-1, respectively in the IR spectra) for characterization of alkane structure, geochemical properties and viscosity of 76 oil samples was performed. These oils, originating from 13 Serbian oil fields in SE Pannonian Basin, differ according to source and depositional environment of organic matter (OM), as well as by thermal maturity and biodegradation stage. Methylene and methyl asymmetric stretching peak absorban

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Zhang, Y., and R. M. Miller. "Effect of Rhamnolipid (Biosurfactant) Structure on Solubilization and Biodegradation of n-Alkanes." Applied and environmental microbiology 61, no. 6 (1995): 2247–51. http://dx.doi.org/10.1128/aem.61.6.2247-2251.1995.

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Elshafie, Abdulkadir, Abdulaziz Yahya AlKindi, Sultan Al-Busaidi, Charles Bakheit, and S. N. Albahry. "Biodegradation of crude oil and n-alkanes by fungi isolated from Oman." Marine Pollution Bulletin 54, no. 11 (2007): 1692–96. http://dx.doi.org/10.1016/j.marpolbul.2007.06.006.

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Liu, Yuan, Yun Yang Wan, Chunjiang Wang, Zheyu Ma, Xiaoli Liu, and Shengjin Li. "Biodegradation of n-alkanes in crude oil by three identified bacterial strains." Fuel 275 (September 2020): 117897. http://dx.doi.org/10.1016/j.fuel.2020.117897.

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Samkov, A. A., N. N. Volchenko, T. N. Musorina, M. N. Kruglova, S. M. Samkova, and A. A. Khudokormov. "Biodegradation of n-Alkanes in Oil-Contaminated Bottom Sediments under Bioelectrochemical Stimulation." Microbiology 93, no. 3 (2024): 314–23. http://dx.doi.org/10.1134/s0026261723602804.

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Weise, Andrea Marga, and Kenneth Lee. "THE EFFECT OF CLAY-OIL FLOCCULATION ON NATURAL OIL DEGRADATION." International Oil Spill Conference Proceedings 1997, no. 1 (1997): 955–56. http://dx.doi.org/10.7901/2169-3358-1997-1-955.

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ABSTRACT Small-scale shaker flask experiments were conducted over 56 days with a weathered crude oil to investigate the significance of clay-oil flocculation processes on natural oil degradation rates. Clay-oil flocculation processes mediated the transfer of oil from solid surfaces into the aqueous phase where oil biodegradation rates are higher. As a result, within 7 days, 34% of the total n-alkanes (n-C12 to n-C35) were degraded in the oiled flasks containing mineral fines whereas no significant degradation was observed in flasks without mineral fines. After 56 days, only 25% and 48% of the

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Teramoto, Maki, Masahito Suzuki, Fumiyoshi Okazaki, Ariani Hatmanti, and Shigeaki Harayama. "Oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment." Microbiology 155, no. 10 (2009): 3362–70. http://dx.doi.org/10.1099/mic.0.030411-0.

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Petroleum-hydrocarbon-degrading bacteria were obtained after enrichment on crude oil (as a ‘chocolate mousse’) in a continuous supply of Indonesian seawater amended with nitrogen, phosphorus and iron nutrients. They were related to Alcanivorax and Marinobacter strains, which are ubiquitous petroleum-hydrocarbon-degrading bacteria in marine environments, and to Oceanobacter kriegii (96.4–96.5 % similarities in almost full-length 16S rRNA gene sequences). The Oceanobacter-related bacteria showed high n-alkane-degrading activity, comparable to that of Alcanivorax borkumensis strain SK2. On the ot

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Zhou, Zhuo, Cuijing Zhang, Pengfei Liu, et al. "Non-syntrophic Methanogenic Hydrocarbon Degradation by an Archaeal Species." ARPHA Conference Abstracts 6 (October 18, 2023): e111614. https://doi.org/10.3897/aca.6.e111614.

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Methanogenic hydrocarbon biodegradation alters the composition of many subsurface oil reservoirs (Jones et al. 2007). This process reduced the crude oil quality by removing alkanes and thus increasing the oil viscosity. The process has been described for syntrophic associations of hydrocarbon-degrading bacteria and methanogenic archaea (Zengler et al. 1999, Dolfing et al. 2007). However, recent culture-independent studies suggest that the archaeon '<em>Candidatus</em> Methanoliparum' may combine alkane degradation and methanogenesis (Laso-Pérez et al. 2019, Borrel et al. 2019). Here we culture

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Ji, Jia-Heng, Yi-Fan Liu, Lei Zhou, et al. "Methanogenic biodegradation of C13 and C14 n-alkanes activated by addition to fumarate." International Biodeterioration & Biodegradation 153 (September 2020): 104994. http://dx.doi.org/10.1016/j.ibiod.2020.104994.

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Suzuki, Miwa, Takuya Hayashi, Kohei Takahashi, Kyoko Nozaki, and Ken-ichi Kasuya. "Exploring biodegradation limits of n-alkanes as polyethylene models using multi-omics approaches." Science of The Total Environment 977 (May 2025): 179365. https://doi.org/10.1016/j.scitotenv.2025.179365.

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Mittal, Anupama, and Padma Singh. "Studies on biodegradation of crude oil by Aspergillus niger." South Pacific Journal of Natural and Applied Sciences 27, no. 1 (2009): 57. http://dx.doi.org/10.1071/sp09010.

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Hydrocarbon degrading microorganisms play a major role in the environment. In the present study crude oil degrading fungal strain Aspergillus niger was isolated from oil contaminated soil near crude oil production well (Lingala) Andhra Pradesh. The rate of reduction in some petroleum hydrocarbon fractions, such as n-alkanes, aromatics, nitrogen, sulfur and oxygen (NSO)-containing compounds and polycyclic aromatic hydrocarbons (PAHs), were monitored by means of gas chromatography. The nC17/Pristine and nC18/Phytane ratios, extrapolated from the GC profiles decreased from the initial value of 2.

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Musat, Florin, Carsten Vogt, and Hans H. Richnow. "Carbon and Hydrogen Stable Isotope Fractionation Associated with the Aerobic and Anaerobic Degradation of Saturated and Alkylated Aromatic Hydrocarbons." Journal of Molecular Microbiology and Biotechnology 26, no. 1-3 (2016): 211–26. http://dx.doi.org/10.1159/000442161.

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Saturated hydrocarbons (alkanes) and alkylated aromatic hydrocarbons are abundant environmental compounds. Hydrocarbons are primarily removed from the environment by biodegradation, a process usually associated with moderate carbon and significant hydrogen isotope fractionation allowing monitoring of biodegradation processes in the environment. Here, we review the carbon and hydrogen stable isotope fractionation associated with the cleavage of C-H bonds at alkyl chains of hydrocarbons. Propane, <i>n</i>-butane and ethylbenzene were used as model components for alkyl moieties of ali

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Samkov, A. A., N. N. Volchenko, T. N. Musorina, M. N. Kruglova, S. M. Samkova, and A. A. Khudokormov. "Biodegradation of <i>n</i>-Alkanes in Oil-Contaminated Bottom Sediments under Bioelectrochemical Stimulation." Microbiology 93, no. 3 (2024): 312–22. http://dx.doi.org/10.31857/s0026365624030066.

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Degradation of oil hydrocarbons artificially introduced into bottom sediments in a bioelectrochemical system of a membrane-free (silt) type was studied. Passive bioelectrochemical stimulation by means of electrodes connected by an external circuit with a resistance of 1 kΩ, with an average electric current of ~85 µA was found to cause an increase in degradation during two months from 23.0 to 57.9%. Contamination of bottom sediments with oil (1.32 g/kg) slightly decreased the current in the external circuit of the bioelectrochemical system. The relationship was revealed between the degree of oi

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39

Karimi, M., and D. Biria. "The synergetic effect of starch and alpha amylase on the biodegradation of n-alkanes." Chemosphere 152 (June 2016): 166–72. http://dx.doi.org/10.1016/j.chemosphere.2016.02.120.

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Östberg, Tomas L., Anders P. Jonsson, and Ulla S. Lundström. "Accelerated biodegradation of n-alkanes in aqueous solution by the addition of fermented whey." International Biodeterioration & Biodegradation 57, no. 3 (2006): 190–94. http://dx.doi.org/10.1016/j.ibiod.2006.01.006.

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Svarovskaya, L. I., and L. K. Altunina. "Biodestruction of Petroleum Hydrocarbons." Eurasian Chemico-Technological Journal 3, no. 2 (2017): 125. http://dx.doi.org/10.18321/ectj555.

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Biodegradation of light and high-viscosity oils by hydrocarbon-oxidizing microflora has been studied. Microflora was isolated from the formation waters recovered from West Siberian and “White Tiger” (Vietnam) oil fields. To activate microorganisms one used the solution of IKhN-KA system containing a multi-component nitrogenous nutrient substrate. Under the condition of active development of microorganisms during 5 days of biodegradation the concentration of n-alkanes C10-C32 in light oils decreased by 70-85 % and that of viscous oil – by 86-93 %. Destruction of mono-aromatic compounds accounte

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Burns, B. J., T. R. Bostwick, and J. K. Emmett. "GIPPSLAND TERRESTRIAL OILS — RECOGNITION OF COMPOSITIONAL VARIATIONS DUE TO MATURITY AND BIODEGRADATION EFFECTS." APPEA Journal 27, no. 1 (1987): 73. http://dx.doi.org/10.1071/aj86008.

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The Gippsland oils, though derived from a common terrestrial source, show considerable variation in their chemical compositions. They range from being very waxy and paraffinic to light, almost condensate-like, oils. Much of this variation can be explained as a function of increasing maturity at the time of generation, with the earliest generated oil being characterised by dominant n-alkanes in the C22-23 is range, remnant odd-over-even preference above C25 and general lack of lower molecular weight gasoline and kerosene range hydrocarbons. At peak generation, the oils show a trimodal distribut

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Burgan, Amer. "Organic Geochemical Evaluation of the Black Shale interbeds Within the Lithological Succession of the Al Aziziyah Formation, NW Libya." Journal of Earth Energy Engineering 12, no. 3 (2023): 96–113. http://dx.doi.org/10.25299/jeee.2023.14037.

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The samples under investigation represent the black shale in interbeds within the Al Aziziyah Formation, located in the NW part of Libya and were analysed using geochemical techniques method and a variety of organic geochemical parameters. The aim of this study is to assess the type of organic matter, thermal maturity, and environment of deposition, based on biomarker distributions and Rock-Eval pyrolysis. The Gas chromatograms of the saturated hydrocarbon fractions of the study samples display a smooth high-end member distribution of the n- n-alkanes extending beyond nC34. The sample displaye

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Bouchard, Daniel, Daniel Hunkeler, and Patrick Höhener. "Carbon isotope fractionation during aerobic biodegradation of n-alkanes and aromatic compounds in unsaturated sand." Organic Geochemistry 39, no. 1 (2008): 23–33. http://dx.doi.org/10.1016/j.orggeochem.2007.10.002.

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Mohanty, Gita, and Suparna Mukherji. "Biodegradation rate of diesel range n-alkanes by bacterial cultures Exiguobacterium aurantiacum and Burkholderia cepacia." International Biodeterioration & Biodegradation 61, no. 3 (2008): 240–50. http://dx.doi.org/10.1016/j.ibiod.2007.06.011.

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Margesin, Rosa, Christoph Moertelmaier, and Johannes Mair. "Low-temperature biodegradation of petroleum hydrocarbons (n-alkanes, phenol, anthracene, pyrene) by four actinobacterial strains." International Biodeterioration & Biodegradation 84 (October 2013): 185–91. http://dx.doi.org/10.1016/j.ibiod.2012.05.004.

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Nasir, Shagufta, and Tahira Fazeelat. "Diamondoid Hydrocarbons as Maturity Indicators for Condensates from Southern Indus Basin, Pakistan." Journal of Chemistry 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/636845.

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Diamondoid hydrocarbons have been examined in condensates reservoired in the Southern Indus Basin using GC-MS. Bulk properties reveal that samples are waxy and low sulfur with the exception of Pakhro and Gopang which are nonwaxy. TIC show bimodal distribution ofn-alkanes along with high abundance of C20+n-alkanes indicating substantial contribution of terrigeneous OM in these samples. CPI close to one is consistent with mature nature of oils. The samples show two ranges of Pr/Ph ratios. Those within the range of 2.2–2.7 reflect marine depositional settings for OM while others with Pr/Ph >3

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Langsa, Markus Heryanto. "Assessing Biodegradation Susceptibilities of Selected Petroleum Hydrocarbons at Contaminated Soils." JOURNAL OF TROPICAL SOILS 15, no. 1 (2018): 39. http://dx.doi.org/10.5400/jts.2010.v15i1.39-47.

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Assessing Biodegradation Susceptibilities of Selected Petroleum Hydrocarbons at Contaminated Soils (M.H. Langsa): The susceptibility to biodegradation of selected saturated hydrocarbons (SHCs), polycyclic aromatic hydrocarbons (PAHs) and asphaltenes in a Barrow crude oil and extracts isolated from soils contaminated with the Barrow crude oil at day 0 and 39 was determined. Soil samples were contaminated with a Barrow crude oil across the surface (5% w/w) as part of a mesocosm experiment in order to mimic similar conditions in the environment. The extent of biodegradation of the Barrow oil extr

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Alimzhanova, Mereke, and Bauyrzhan Abdykarimov. "Analysis of Kazakhstan Crude Oil Biomarkers by Gas Chromatography in Combination with Mass Spectrometry." Separations 10, no. 11 (2023): 561. http://dx.doi.org/10.3390/separations10110561.

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Kazakhstan ranks as the 12th largest oil producer globally and boasts a diverse range of crude oils. This research introduces a method for distinguishing between the different types of crude oils based on biomarker analysis of 28 crude oils from Western and Southern Kazakhstan using gas chromatography-mass spectrometry. Biomarkers serve as valuable tools, especially in forensic investigations of oil spills. These biomarkers effectively retain a significant portion of the original natural product’s carbon structure, providing crucial evidence regarding the origin and identity of the oils under

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Arabo, Abdulrahman Abdulhamid, Raji Arabi Bamanga, Mujiburrahman Fadilu, et al. "Isolation and Characterization of Biosurfactant-producing Alcaligenes sp. YLA11 and its Diesel Degradation Potentials." Bioremediation Science and Technology Research 9, no. 2 (2021): 7–12. http://dx.doi.org/10.54987/bstr.v9i2.619.

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This study aimed to isolate and identify biosurfactant producing and diesel alkanes degrading bacteria. For this reason, bacteria isolated from the diesel contaminated site were screened for their potential to produce biosurfactants and degrade diesel alkanes. Primary selection of diesel degraders was carried out by using conventional enrichment culture technique where 12 bacterial strains were isolated based on their ability to grow on minimal media supplemented with diesel as sole carbon source, which was followed by qualitative screening methods for potential biosurfactant production. Isola

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