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Journal articles on the topic 'N6-benzylaminopurine'

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Published: 3 June 2025

Last updated: 31 July 2025

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1

Chen, Yongqin, and Chitrapan Piluek. "Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis." Plant Growth Regulation 16, no. 1 (1995): 99–101. http://dx.doi.org/10.1007/bf00040513.

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2

Wickremesinhe, E. R. M., W. J. Blackmon, and B. D. Reynolds. "In Vitro Propagation of Apios americana." HortScience 25, no. 11 (1990): 1439–40. http://dx.doi.org/10.21273/hortsci.25.11.1439.

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Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

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3

Blahut-Beatty, Laureen M., Peta C. Bonham-Smith, and Vipen K. Sawhney. "Induction of "filamentous structures" in wild type Antirrhinum majus flowers by benzylaminopurine." Canadian Journal of Botany 76, no. 11 (1998): 1828–34. http://dx.doi.org/10.1139/b98-152.

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The cytokinin, N6-benzylaminopurine (BAP), when applied to young inflorescences of wild-type Antirrhinum majus L., resulted in the formation of chimeric "filamentous structures" in the dorsal region of the third whorl, the position where a stamen primordium is suppressed in wild-type flowers. In addition, BAP induced similar filamentous structures in between the first and second whorls, and this response was concentration dependent. The basal region of the filamentous structures was similar to the filament of a stamen, while the distal portion resembled a petal. These observations suggest that

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4

McLaughlin, J., and D. F. Karnosky. "Controlling vitrification in Larixdecidua via culture media manipulation." Canadian Journal of Forest Research 19, no. 10 (1989): 1334–37. http://dx.doi.org/10.1139/x89-205.

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Vitrification rates obtained from our inverted embryo system were significantly decreased by lowering the cytokinin concentrations in Brown and Lawrence medium containing 10 mM L-glutamine from 10 to 1 mg/L, or by replacing 10 mM L-glutamine with equimolar concentrations of Ca(NO3)2 or by adding 1 g/L Gelrite to the normal 10 g/L Difco–Bacto agar in the culture media. In all treatments, decreased vitrification was accompanied with decreased adventitious bud production. With reduced N6-benzylaminopurine, vitrification decreased from 77 to 29%, but bud production decreased from 61 to 17 buds per

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5

Zaluzec, Eugene J., Marianne M. Zaluzec, Kenneth W. Olsen, and Stephen F. Pavkovic. "Crystallization and preliminary X-ray analysis of peanut agglutinin-N6-benzylaminopurine complex." Journal of Molecular Biology 219, no. 2 (1991): 151–53. http://dx.doi.org/10.1016/0022-2836(91)90556-l.

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6

Burger, David W. "In Vitro Micropropagation of Eucalyptus sideroxylon." HortScience 22, no. 3 (1987): 496–97. http://dx.doi.org/10.21273/hortsci.22.3.496.

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Abstract Nodes from field-grown coppice regrowth and mature trees of Eucalyptus sideroxylon were cultured on a modified Murashige and Skoog medium containing BAP and NAA. BAP (2-4 μm) and NAA (0.5-1.0 μm) were most effective in promoting axillary shoot proliferation from coppice and adult tissues. IBA was more effective than NAA in stimulating the formation of adventitious roots from in vitro-derived shoots. Plantlets from tissue culture were easily transferred to the greenhouse environment. Chemical names used: N6-benzylaminopurine (BAP); 1-naphthaleneacetic acid (NAA); 1H-indole-3-butanoic a

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7

Prins, Claudia Lopes, Silvério de Paiva Freitas, Mara de Menezes de Assis Gomes, Ivo José Curcino Vieira, and Geraldo de Amaral Gravina. "Citral accumulation in Cymbopogon citratus plant as influenced by N6-benzylaminopurine and light intensity." Theoretical and Experimental Plant Physiology 25, no. 2 (2013): 159–65. http://dx.doi.org/10.1590/s2197-00252013000200008.

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8

Raghavan, V., and R. Nagmani. "Cytokinin effects on pollen embryogenesis in cultured anthers of Hyoscyamus niger." Canadian Journal of Botany 67, no. 1 (1989): 247–57. http://dx.doi.org/10.1139/b89-034.

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Addition of the cytokinins, benzylaminopurine, N6-Δ-(2-isopentenyl)-adenine, kinetin, zeatin, and zeatin riboside to a basal medium containing mineral salts and sucrose induced characteristic changes in pollen embryogenesis in cultured anthers and anther segments of Hyoscyamus niger. In anthers cultured in media containing 0.01–10.0 mg/L of each cytokinin, there was a progressive delay in the appearance of plantlets outside the anther locule and in the morphology of plantlets formed. Among the cytokinins tested, only zeatin riboside promoted anther efficiency; however, all compounds reduced po

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9

Zhang, Ping, Li M. Wang, Da W. Zheng, et al. "Surface-enhanced Raman spectroscopic analysis of N6-benzylaminopurine residue quantity in sprouts with gold nanoparticles." Journal of Environmental Science and Health, Part B 53, no. 9 (2018): 561–66. http://dx.doi.org/10.1080/03601234.2018.1473954.

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10

Letham, David S., Noel G. Smith, and David A. Willcocks. "Cytokinin metabolism in Narcissus bulbs: chilling promotes acetylation of zeatin riboside." Functional Plant Biology 30, no. 5 (2003): 525. http://dx.doi.org/10.1071/fp02097.

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The levels of the cytokinins zeatin, dihydrozeatin and N6-(isopent-2-enyl)adenine and the corresponding 9-ribosides were determined in whole Narcissus pseudonarcissus L. bulbs after onset of summer dormancy, and after storage of the dormant bulbs at 4 or 15°C under moist conditions. The cytokinin level was increased at both temperatures; the dominant cytokinin at 15°C was zeatin riboside but at 4°C the free base, zeatin, was the principal cytokinin. The metabolism of 3H-labelled zeatin, zeatin riboside and 6-benzylaminopurine was studied in scales and base plates excised from bulbs previously

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11

Liew, T. K., and C. K. H. Teo. "Multiple Shoot Production In Vitro of the Tropical Timber Tree, Sentang (Azadirachta excelsa Linn.)." HortScience 33, no. 6 (1998): 1073–75. http://dx.doi.org/10.21273/hortsci.33.6.1073.

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Axillary buds from 5-month-old seedlings of Azadirachta excelsa Linn. were surface-sterilized twice with 1.35% (m/v) and 1.05% (m/v) of sodium hypochlorite for 25 and 15 minutes, respectively, before culturing on Murashige and Skoog (MS) medium containing combinations of BA and NAA. A combination of 4.4 μM BA + 0.5 μM NAA induced the most axillary buds to grow (eight per explant). Subsequent proliferation of the micropropagated shoots on this medium yielded abnormal shoots. The best medium for maximum proliferation of these micropropagated shoots contained 3.3 μM BA and 0.27 μM NAA. On this me

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12

Maloň, Michal, Zdeněk Trávnı́ček, Miroslav Maryško, et al. "Metal complexes as anticancer agents 2. Iron(III) and copper(II) bio-active complexes with N6-benzylaminopurine derivatives." Inorganica Chimica Acta 323, no. 1-2 (2001): 119–29. http://dx.doi.org/10.1016/s0020-1693(01)00611-9.

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13

Gambley, RL, JD Bryant, NP Masel, and GR Smith. "Cytokinin-Enhanced Regeneration of Plants From Microprojectile Bombarded Sugarcane Meristematic Tissue." Functional Plant Biology 21, no. 5 (1994): 603. http://dx.doi.org/10.1071/pp9940603.

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Sugarcane plants expressing the firefly luciferase gene were regenerated from microprojectile-bombarded meristematic tissue. The concentration and type of cytokinin added to the basal media, both prior to bombardment and during regeneration, significantly affected the number of plantlets produced and the percentage of plantlets expressing luciferase. Maximum shoot initiation from meristematic tissue of sugarcane cultivar Q137 was achieved on media supplemented with N6-benzylaminopurine (BAP). There were differences in the numbers of shoots initiated from different sugarcane cultivars, and some

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14

PALEE, Jiraporn. "In vitro Shoot Cultures of Tupistra albiflora K. Larsen." Walailak Journal of Science and Technology (WJST) 17, no. 5 (2018): 405–11. http://dx.doi.org/10.48048/wjst.2020.4182.

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To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the

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15

Gill, Ravinder, Jean M. Gerrath, and Praveen K. Saxena. "High-frequency direct somatic embryogenesis in thin layer cultures of hybrid seed geranium (Pelargonium ×hortorum)." Canadian Journal of Botany 71, no. 3 (1993): 408–13. http://dx.doi.org/10.1139/b93-045.

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Thin layer explants from the hypocotyls of young seedlings of geranium (Pelargonium ×hortorum Bailey) produced somatic embryos in response to thidiazuron (TDZ) or a combination of indoleacetic acid and N6-benzylaminopurine, but the number of embryos was much less in the latter than with TDZ. The development of somatic embryos in cultures of thin layers was rapid and the number of embryos was about eightfold higher than in cultures of whole hypocotyl explants. Thin layer explants of 1-week-old seedlings produced a high number of somatic embryos than those obtained from older seedlings. The cult

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16

Shi, Guomin, Lina Yang, and Tao He. "Plant regeneration from protoplasts of Gentiana straminea Maxim." Open Life Sciences 11, no. 1 (2016): 55–60. http://dx.doi.org/10.1515/biol-2016-0007.

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AbstractA protocol is described for plant regeneration from protoplasts of Gentiana straminea Maxim. via somatic embryogenesis. Protoplasts were isolated from embryogenic calli in an enzyme solution composed of 2% Cellulase Onozuka R-10, 0.5% Macerozyme R-10, 0.5% Hemicellulase, and 0.5 M sorbitol with a yield of 3.0 × 106 protoplasts per gram of fresh weight. Liquid, solid-liquid double layer (sLD) and agar-pool (aPL) culture systems were used for protoplast culture. The aPL culture was the only method that produced embryogenic, regenerative calli. With aPL culture, the highest frequencies of

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17

Gill, R., and Praveen K. Saxena. "Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron." Canadian Journal of Botany 70, no. 6 (1992): 1186–92. http://dx.doi.org/10.1139/b92-147.

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An efficient procedure has been developed for inducing direct somatic embryogenesis, organogenesis, and regeneration of plants from tissue cultures of peanut (Arachis hypogaea L.). Thin transverse sections of the cotyledons and juvenile leaves were cultured on Murashige and Skoog medium supplemented with N6-benzylaminopurine (BAP) or a substituted phenylurea, thidiazuron (TDZ). Somatic embryos or shoot buds differentiated from cut surfaces of the cotyledons and midrib region of the leaves. The application of BAP induced differentiation of shoot buds whereas the treatment with TDZ resulted in t

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18

Tan, Meimei, Ruoxin Chen, Xingran Chen, Muhammad Qasim Shahid, Xiangdong Liu, and Jinwen Wu. "In Vitro Induction of Interspecific Hybrid and Polyploidy Derived from Oryza officinalis Wall." Plants 12, no. 16 (2023): 3001. http://dx.doi.org/10.3390/plants12163001.

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Oryza officinalis Wall is a potential genetic resource for rice breeding; however, its distant genome limits its crossing ability with cultivated rice. The interspecific hybridization of O. officinalis and cultivated rice, establishment of its tissue culture, and induction of polyploidy are ways to improve O. officinalis’s poor crossability. We developed an interspecific hybrid and studied its reproductive pollen development process in this work, and the results showed that abortive pollens (81.94%) and embryo sac abnormalities (91.04%) were the key causes of its high sterility. In order to in

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19

Yu, Rongpei, Ying Cheng, Yanfei Pu, Fan Li, and Shugang Lu. "In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants." HortScience 56, no. 3 (2021): 313–17. http://dx.doi.org/10.21273/hortsci15546-20.

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The resurrection plant Selaginella pulvinata (Hook. & Grev.) Maxim is used as an ornamental and medicinal plant. It is also a good candidate for exploring the desiccation tolerance of resurrection plants. However, there is not an efficient propagation method for S. pulvinata. In the present study, we evaluated the establishment of in vitro propagation of S. pulvinata using frond tips as explants. The original shoot induction, adventitious shoot proliferation and plantlet growth media, and substrate type of plantlet acclimatization were investigated. The highest induction rate of original s

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20

Catalano, Caterina, Loredana Abbate, Antonio Motisi, et al. "Autotetraploid Emergence via Somatic Embryogenesis in Vitis vinifera Induces Marked Morphological Changes in Shoots, Mature Leaves, and Stomata." Cells 10, no. 6 (2021): 1336. http://dx.doi.org/10.3390/cells10061336.

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Polyploidy plays an important role in plant adaptation to biotic and abiotic stresses. Alterations of the ploidy in grapevine plants regenerated via somatic embryogenesis (SE) may provide a source of genetic variability useful for the improvement of agronomic characteristics of crops. In the grapevine, the SE induction process may cause ploidy changes without alterations in DNA profile. In the present research, tetraploid plants were observed for 9.3% of ‘Frappato’ grapevine somatic embryos regenerated in medium supplemented with the growth regulators β-naphthoxyacetic acid (10 µM) and N6-benz

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21

Owen, Henry R., and Louis H. Aung. "Genotypic and Chemical Influences on Fruit Growth of Tomato." HortScience 25, no. 10 (1990): 1255–57. http://dx.doi.org/10.21273/hortsci.25.10.1255.

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A relationship between ovary size at anthesis and final fruit diameter of 12 tomato (Lycopersicon esculentum Mill.) cultivars with a range of fruit sizes, shapes, and maturation rates was determined. `Fireball', `Michigan/Ohio Hybrid', and `New Yorker' produced nonfasciated, spherical fruits of intermediate maturation rate and showed a significantly higher correlation between ovary diameter at anthesis and final fruit diameter than `Small Fry', `Roma VF', `Early Cascade', `Campbell 1327', or `Ponderosa'. A linear regression of final fruit diameter at maturity on ovary diameter at anthesis of t

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22

Depetris, Mara B., Adam M. Dimech, and Kathryn M. Guthridge. "Digitally Quantifying Growth and Verdancy of Lolium Plants In Vitro." Plants 14, no. 10 (2025): 1499. https://doi.org/10.3390/plants14101499.

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The image analysis of plants provides an opportunity to measure changes in growth and physiology quantitatively, and non-destructively, over time providing significant advantages over traditional methods of assessment which often rely on qualitative and subjective measures to distinguish between different treatments or genotypes in an experiment. Image analysis techniques are commonly deployed for the analysis of plants in the field or glasshouse, but few studies have demonstrated the use of image analysis to phenotype plants grown under aseptic conditions in culture media. Lolium × hybridum H

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23

Varisai Mohamed, Shamsudeen, Jih-Min Sung, Toong-Long Jeng, and Chang-Sheng Wang. "Organogenesis of Phaseolus angularis L.: high efficiency of adventitious shoot regeneration from etiolated seedlings in the presence of N6-benzylaminopurine and thidiazuron." Plant Cell, Tissue and Organ Culture 86, no. 2 (2006): 187–99. http://dx.doi.org/10.1007/s11240-006-9107-1.

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24

M, Manokari, Jayaprakash K, Cokul Raj M, et al. "In vitro micro-morphometric growth modulations induced by N6 cytokinins (Meta-Topolin and 6-benzylaminopurine) in Ceropegia juncea Roxb. – A rare medicinal climber." South African Journal of Botany 157 (June 2023): 656–66. http://dx.doi.org/10.1016/j.sajb.2023.04.045.

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25

Nasri, Fardin, Seyed Najmedin Mortazavi, Nasser Ghaderi, and Taimoor Javadi. "Propagation in vitro of alstroemeria ligtu hybrid through direct organogenesis from leaf base." Journal of Horticultural Research 21, no. 2 (2013): 23–30. http://dx.doi.org/10.2478/johr-2013-0018.

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ABSTRACT In the present study, multiplication efficiency of Alstroemeria ligtu hybrid was investigated. Bases of the first seedling leaves grown in vitro were used as initial explants. The explants were cultured in the MS media containing 3% sucrose, 0.7% agar at pH 5.8, five N6-benzylaminopurine (BAP) concentrations (0, 0.5, 1, 1.5 and 2 mg·1-1) and three indole-3-butyric acid (IBA) concentrations (0, 0.1 and 0.2 mg·1-1). The cultures were incubated at 21 ± 2 °C under photoperiod 16/8. After three subculturings (3 weeks-long each) the number of rhizome, shoots, buds, leaves, and roots, length

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26

Gonzales-Alvarado, Antony Cristhian, and Jean Carlos Cardoso. "Development, Chlorophyll Content, and Nutrient Accumulation in In Vitro Shoots of Melaleuca alternifolia under Light Wavelengths and 6-BAP." Plants 13, no. 20 (2024): 2842. http://dx.doi.org/10.3390/plants13202842.

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In vitro cultivation of Melaleuca could contribute to the cloning of superior genotypes. Studies of factors affecting micropropagation are needed, such as the interaction with light-emitting diodes (LEDs) and plant growth regulators added to the culture media. This study aimed at better understanding the effects of spectra on the development and physiology of melaleuca cultivated in vitro, as well as the interaction of LEDs with the main cytokinin used in micropropagation, N6-Benzylaminopurine (6-BAP). 6-BAP, spectra, and their interaction had a significant effect on most of the variables anal

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27

Ricci, Angela, Luca Capriotti, Bruno Mezzetti, Oriano Navacchi, and Silvia Sabbadini. "Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536." Plants 9, no. 6 (2020): 755. http://dx.doi.org/10.3390/plants9060755.

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In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP).

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28

Percy, Olivera-Gonzales, M. Yldefonzo Edson, M. Mestanza Elder, and Tamariz-Angeles Carmen. "In vitro Propagation of Oreocallis grandiflora (Lam.) R. Br., A Medicinal Threatened Plant." Annual Research & Review in Biology 14, no. 3 (2017): 1–9. https://doi.org/10.9734/ARRB/2017/34724.

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<strong>Aims: </strong>To promote the conservation and biotechnology approach of <em>Oreocallis grandiflora</em>, a promising wild, medicinal and agroforestry Peruvian plant, a methodology for its <em>in vitro</em> propagation was performed. <strong>Study Design:</strong> Disinfection and germination of seed, followed by shoot multiplication and rooting using <em>in vitro</em> techniques. <strong>Place and Duration of Study:</strong> Follicles were collected in Yupa - Huaraz (9°30'26.76"S, 77°28'21.33"W) and the experimental procedure was done between January 2013 and December 2016. <strong>Me

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29

Tasheva, Krasimira, and Georgina Kosturkova. "Bulgarian golden root in vitro cultures for micropropagation and reintroduction." Open Life Sciences 5, no. 6 (2010): 853–63. http://dx.doi.org/10.2478/s11535-010-0092-3.

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AbstractRhodiola rosea is an endangered medicinal plant used for cancer, cardiovascular, and nervous system diseases therapy. Due to its limited distribution and sustainability alternative methods for production of its valuable substances are under investigation. Using in vitro techniques apical and rhizome buds, leaf nodes, stem and radix segments from wild plants and in vitro seedlings were plated on 24 modified Murashige and Skoog (1962) media. Decontamination of plant material was successful only in 21% of the schemes. The best shoot induction was obtained from seedling explants on media c

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30

Drew, R. A. "Rapid Clonal Propagation of Papaya in Vitro from Mature Field-grown Trees." HortScience 23, no. 3 (1988): 609–11. http://dx.doi.org/10.21273/hortsci.23.3.609.

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Abstract Rapid development of axillary branches on rooted cuttings from mature field-grown papaya (Carica papaya L.) trees was promoted by removal of stem apices and application to the cut surface of a mixture containing 225 mg BAP/liter of lanolin. Small axillary buds were removed from these branches and cultured, initially on solid medium, and then in liquid medium on a roller drum at 3 rpm in Drew and Smith (DS) medium containing 1 µm BAP + 1 µm NAA. After two or three subcultures on the roller drum with alternate periods on hormone-free basal medium, apically dominant shoots were produced.

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31

PRAMANIK, Dewi, Muhammad PRAMA YUFDY, Herni SHINTIAVIRA, and Budi WINARTO. "In Vitro Secondary Embryogenesis Derived from Meta-Topoline Treatment on Mass Propagation of Phalaenopsis ‘AMP 17’." Notulae Scientia Biologicae 8, no. 1 (2016): 62–68. http://dx.doi.org/10.15835/nsb819692.

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Phalaenopsis ‘AMP 17’ is an important orchid commodity in Indonesia with high market demand; however, scaling up the orchid commercially is constrained by the availability and sustainability of qualified seedlings. To overcome the problem, a reliable in vitro propagation protocol, especially via secondary embryogenesis, was undertaken. In the present study, in vitro secondary embryogenesis derived from meta-topoline (mT) treatment on mass propagation of Phalaenopsis ‘AMP 17’ was successfully established. Embryos, as explant sources, were prepared by culturing meristem tips of flower stalk shoo

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32

TORRES, GUSTAVO RUBENS DE CASTRO, FLAVIA PAIVA COUTINHO, BIANCA GALÚCIO PEREIRA ARAÚJO, GIOVANNA PEREIRA DE ABREU, and RUI SALES JÚNIOR. "THERMOTHERAPY AS A MICROBIAL CONTAMINANT-REDUCING AGENT IN MICROPROPAGATION OF BAMBOO." Revista Caatinga 32, no. 3 (2019): 690–97. http://dx.doi.org/10.1590/1983-21252019v32n313rc.

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ABSTRACT The aim of the research was to evaluate the effectiveness of thermotherapy by humid heat of nodal segments to reduce the incidence of microbial contaminants in the introduction phase of the in vitro propagation of Bambusa vulgaris. Two independent experiments were carried out in a completely randomized design using a 4 × 2 factorial arrangement, four levels of temperature (25, 30, 40 and 50 °C) versus two treatment time intervals (5 or 10 min). Each treatment was composed of nine replications, corresponding to one single-nodal segment obtained from primary or secondary branches, in su

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33

Lai, I.-Ling, Chih-Wan Lin, Tsai-Yu Chen, and Wei-Hsin Hu. "Micropropagation Shortens the Time to Blooming of Begonia montaniformis × Begonia ningmingensis var. bella F1 Progeny." HortScience 53, no. 12 (2018): 1855–61. http://dx.doi.org/10.21273/hortsci13376-18.

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Begonia montaniformis × Begonia ningmingensis var. bella hybrids have high ornamental potential. Hence, the aim of this study was to determine the optimal conditions for the micropropagation of a Begonia montaniformis × Begonia ningmingensis var. bella F1 progeny by using various concentrations of plant growth regulators (PGRs) and varying light spectra in half-strength Murashige and Skoog (1/2 MS) medium. The results showed that the explant regeneration was optimal when the lamina was incubated in a medium supplemented with 2.0 μM N6-benzylaminopurine and 0.8 μM α-naphthaleneacetic acid (NAA)

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34

Akram, Muhammad, and Faheem Aftab. "Callus induction and chemical characterization of cell suspension cultures of jojoba (Simmondsia chinensis L.)." World Journal of Biology and Biotechnology 6, no. 3 (2021): 5. http://dx.doi.org/10.33865/wjb.006.03.0437.

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Jojoba (Simmondsia chinensis L.) oil is also known as liquid wax or fixed oil. It is an important metabolite of jojoba having commercial importance in cosmetics as well as a potential biofuel source. We presented an efficient system for in vitro establishment of cell suspension cultures (CSC) from proliferating friable calluses. For this purpose, cotyledon, internode, and leaf explants were cultured on MS medium + 1, 2, 4, 6, 8 or 10 µM 2, 4-Dichlorophenoxyacetic acid (2, 4-D), α-Naphthalene acetic acid (NAA) alone or in combination with 1 or 2 µM N6-benzylaminopurine (BAP) or Kinetin. Results

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35

Yan, Hoo Kah, Lee Ping Chin, Mariam A. Latip, Noumie Surugau, and Zaleha A. Aziz. "CALLUS FORMATION AND PLANT REGENERATION FROM IMMATURE EMBRYOS OF PIGMENTED UPLAND RICE (Oryza sativa cv. Tadong)." Jurnal Teknologi 83, no. 4 (2021): 91–100. http://dx.doi.org/10.11113/jurnalteknologi.v83.14518.

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The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot r

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Bahrudin, Nurul Fairuz Diyana, Umar Hamzah, and Wan Zuhairi Wan Yaacob. "ANALISIS SESAR-SESAR MAJOR SEMENANJUNG MALAYSIA DENGAN TEKNIK GRAVITI DEKONVOLUSI EULER 3D." Jurnal Teknologi 83, no. 4 (2021): 137–50. http://dx.doi.org/10.11113/jurnalteknologi.v83.13874.

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The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot r

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Kıymaz, Gizem, and Arda Acemi. "In vitro Propagation of Ornamental Snapdragon (Antirrhinum majus L.) Revisited: An Analysis on the Effects of Plant Growth Regulators." Commagene Journal of Biology 8, no. 1 (2024): 37–45. http://dx.doi.org/10.31594/commagene.1450579.

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The effects of N6-(2-isopentenyl) adenosine (IP) and p-chlorophenoxyacetic acid (CPA) on the propagation of Antirrhinum majus have yet to be elucidated. This study compares plant growth regulators’ effects on enhancing propagation efficiency in snapdragon. In the seedling development phase, IP at 1.0 mg L-1 provided the highest germination ratio (91.11 ± 9.30%). The 6-benzylaminopurine (BAP) treatment at 1.0 mg L-1 gave the highest number of shoots (4.42 ± 0.30) whereas the medium with 0.50 mg L-1 IP triggered shoot elongation (3.80 ± 0.28 cm) from germinating seeds. The medium with 1.0 mg L-1

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BRIAND, CHRISTOPHER H., and GARY S. HICKS. "MICROPROPAGATION OF THE COLD-HARDY APPLE ROOTSTOCK KSC-3: A MORPHOLOGICAL ANALYSIS." Canadian Journal of Plant Science 69, no. 2 (1989): 555–64. http://dx.doi.org/10.4141/cjps89-068.

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The cold-hardy apple rootstock KSC-3 (Malus × domestica Borkh.) was micropropagated on modified Murashige and Skoog (MS) medium using single node explants as the starting material. Explants grown on media containing BAP (0.5–5.0 mg L−1) and zeatin (5.0 mg L−1) produced multiple shoots. The largest number of shoots (≥ 5.0 mm) were obtained with 1.0 mg L−1 BAP. On the basis of useable shoots (≥ 15.0 mm), however, there was no difference in shoot number between 0.5 and 1.0 mg L−1 BAP. With increasing BAP concentration, shoot length decreased, while stem diameter increased. The longest shoots were

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39

Mahakur, Bhaswatımayee, Arpıta Moharana, Sanjay Kumar Madkamı, Soumendra Kumar Naık, and Durga Prasad Barık. "Optimization of factors affecting Agrobacterium-mediated hairy root induction in Vitex negundo L. (Lamiaceae)." International Journal of Secondary Metabolite 11, no. 2 (2024): 244–54. http://dx.doi.org/10.21448/ijsm.1368677.

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Vitex negundo L. is an aromatic, woody, blooming shrub in the Verbenaceae family which can grow into a small tree. Traditionally, V. negundo root has been used to cure diabetes, colic, boils, leprosy, and rheumatism. Keeping the importance of its roots in mind, an attempt has been taken for development of a protocol for efficient hairy root proliferation system. The Agrobacterium rhizogenes strains (MTCC 532 and MTCC 2364) were used for hairy root induction. For A. rhizogenes infection, both in vitro and in vivo leaves as well as internodes were used as explant. In vitro leaves and internodal

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Piotrowska, Alicja, Romuald Czerpak, Joanna Adamowicz, Alicja Biedrzycka, and Marta Potocka. "Comparison of stimulatory effect of cytokinins adenine and urea derivatives on the level of some components in Wolffia arrhiza (L.) Wimm. (Lemnaceae)." Acta Societatis Botanicorum Poloniae 74, no. 2 (2011): 111–18. http://dx.doi.org/10.5586/asbp.2005.015.

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The effect of cytokinins with different chemical structures, adenine-type: N6-benzylaminopurine (BAP), N6-furfurylaminopurine (FAP) and derivative of urea - 1,3-diphenylurea (DPU) applied in optimal physiological concentrations of 10<sup>-6</sup>-10<sup>-4</sup> M on the mixotrophic plant <em>Wolffia arrhiza</em> during the period of 20 days of culture, was examined. The cytokinin highest influence on the metabolites level in <em>W. arrhiza</em> fresh weight was demonstrated in the range of concentrations 10<sup>-6</sup>-10<sup>

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Ghareb, Heba El-Sayed, Shafik Darwish Ibrahim, and Ghada Abd El-Moneim Hegazi. "Micropropagtion and DNA-barcoding of the endangered endemic Phlomis aurea plant of Saint Katherine." MAY 2020, no. 13(01): 2020 (May 20, 2020): 65–77. http://dx.doi.org/10.21475/poj.13.01.20.p2398.

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Saint Katherine is considered a “biodiversity hotspot” because of the high level of endemism of reported plant species. In this study, conservation of the endangered endemic plant; Phlomis aurea of Saint Katherine, Southern Sinai, Egypt, was carried out through micropropagation and DNA barcoding. The first efficient micropropagation protocol for Phlomis aurea was established as a mean of ex situ conservation of the plant. Shoot tips and nodal segments of in vitro germinated seedlings were established on Murashige and Skoog medium supplemented with 0.54 µM β-naphthalene acetic acid (NAA) and 2.

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Palta, J. A., and C. Ludwig. "Pod set and seed yield as affected by cytokinin application and terminal drought in narrow-leafed lupin." Australian Journal of Agricultural Research 48, no. 1 (1997): 81. http://dx.doi.org/10.1071/a96042.

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An individual flower-painting technique that utilises N6-benzylaminopurine (BAP) to increase pod set was used on an indeterminate cultivar of narrow-leafed lupin (Lupinus angustifolius L. cv. Merrit) and on a breeding line with restricted branching (84A/241) to examine the interaction between pod set, water deficit, and seed yield. Petals and sepals of each flower on each inflorescence were painted with a 0·002 M solution of BAP every day from the first day the flower opened to the day it senesced. A water deficit was induced, after flowering on the first-order apical branch, in half of the pl

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Palta, J. A., and C. Ludwig. "Yield response of narrow-leafed lupin plants to variations in pod number." Australian Journal of Agricultural Research 49, no. 1 (1998): 63. http://dx.doi.org/10.1071/a97092.

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The effect of pod number on the seed yield and components of seed yield was examined for narrow-leafed lupin (Lupinus angustifolius L.) grown at a plant density of 36 plants/m2 in both the glasshouse and the field. Diflerent numbers of pods per plant in the glasshouse-grown lupin were generated by the application of N6-benzylaminopurine (BAP) to a specific number of flowers to ensure artificially that they set pods, and the subsequent removal of the remaining untreated flowers. Pod number ranged from 6 to 65 pods/plant in the glasshouse and was naturally distributed from 2 to 22 pods/plant in

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Labidi, Rahma, Chokri Bayoudh, Afifa Majdoub, Margarita Lopez-Corrales, and Messaoud Mars. "In vitro propagation of caprifig and figs (Ficus carica L.) through various explants." Journal of Applied Horticulture 25, no. 03 (2023): 263–68. http://dx.doi.org/10.37855/jah.2023.v25i03.47.

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Fig (Ficus carica L.) cultivation is affronting serious problems caused by biotic and abiotic stresses. Application of in vitro techniques became necessary for plant material sanitation, rapid propagation and conservation. The present study aims the regeneration of in vitro plants of local fig cultivars using different explant types. For that purpose, shoot tips, meristems and leaf segments of 3 local fig cultivars (Soltani, Zidi, Bither Abiadh) and a caprifig (Assafri) were cultivated in vitro. MS media with different concentrations of growth hormones: ?-naphthaleneacetic acid (NAA), indole-3

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Cui, Jin, Juanxu Liu, Jianjun Chen, and Richard J. Henny. "Regeneration of Chlorophytum amaniense ‘Fire Flash’ Through Indirect Shoot Organogenesis." HortScience 46, no. 3 (2011): 466–69. http://dx.doi.org/10.21273/hortsci.46.3.466.

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Chlorophytum amaniense Engl. ‘Fire Flash’ is a popular exotic ornamental foliage plant as a result of its unique coral-colored midribs and petioles and tolerance to interior low light levels. Currently, demand for propagative materials exceeds the availability of seeds. This study was intended to develop an in vitro culture method for rapid propagation of this cultivar. Leaf and sprouted seed explants were cultured on a Murashige and Skoog basal medium supplemented with different cytokinins with 1.1 μM α-naphthalene acetic acid (NAA) or 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Leaf expla

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Kahia, Jane, Peter Kanze Sallah, Lucien Diby, et al. "A Novel Regeneration System for Tamarillo (Cyphomandra betacea) via Organogenesis from Hypocotyl, Leaf, and Root Explants." HortScience 50, no. 9 (2015): 1375–78. http://dx.doi.org/10.21273/hortsci.50.9.1375.

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Cyphomandra betacea (Cav.) is commonly known as Tamarillo or tree tomato. This species is mainly used for its edible fruits which have a high nutritional value and contain relatively high amounts of proteins, vitamins B6, C, E, and provitamin A. The cultivation of Tamarillo in Rwanda is facing major challenges caused mainly by viral diseases such as Tamarillo mosaic virus (TaMV). These diseases are difficult to control and are transferred through vegetative propagation, often resulting in heavy productivity losses and poor-quality fruits. Thus, this study was conducted to evaluate the possibil

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Kahia, Jane, Margaret Kirika, Hudson Lubabali, and Sinclair Mantell. "High-frequency Direct Somatic Embryogenesis and Plantlet Regeneration from Leaves Derived from In Vitro-Germinated Seedlings of a Coffea arabica Hybrid Cultivar." HortScience 51, no. 9 (2016): 1148–52. http://dx.doi.org/10.21273/hortsci10771-16.

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Breeding work carried out during the period 1971–85 by the Coffee Research Institute, Ruiru, Kenya resulted in the release of a new improved hybrid Coffea arabica named Ruiru 11. The cultivar combines resistance to coffee berry disease (CBD) and leaf rust, with high yield and good cup quality attributes. The propagation by F1 hybrid seeds production, cuttings, and tip grafting do not produce enough planting materials. There was a need to explore alternative methods and tissue culture offers potential options. The objective of the study was to evaluate the effect of explant sources and cytokini

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Palta, J. A., and C. Ludwig. "Elevated CO2 during pod filling increased seed yield but not harvest index in indeterminate narrow-leafed lupin." Australian Journal of Agricultural Research 51, no. 2 (2000): 279. http://dx.doi.org/10.1071/ar99099.

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Indeterminate narrow-leafed lupin (Lupinus angustifolius L. cv. Merrit) was exposed to enriched atmospheric CO2 during pod-filling to enhance the availability of carbon resources for pod-filling in order to determine whether or not seed-filling, yield, and harvest index are limited by the availability of photosynthetic assimilate. Plants were grown in a glasshouse and the flowers painted with an aqueous solution containing either N6-benzylaminopurine (BAP) or no BAP to generate 2 different numbers of pods per plant. From the time when pods began to fill seeds (≥5 mg/seed) until maturity, plant

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McAvoy, Richard J., and Bernard B. Bible. "Poinsettia Bract Necrosis Is Affected by Scion/Rootstock Combinations and Arrested by a Benzyladenine Spray." HortScience 33, no. 2 (1998): 242–46. http://dx.doi.org/10.21273/hortsci.33.2.0242.

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Poinsettia (Euphorbia pulcherrima Willd. ex. Klotzch) cultivars vary widely in susceptibility to bract necrosis, a disorder that has been associated with localized calcium deficiency. When `Supjibi Red' (SR) poinsettia shoots were grafted on `SR' stocks, a higher incidence of bract necrosis was observed (26.4% of bracts) 28 days after initial anthesis than on corresponding `SR' shoots grafted on `Annette Hegg Dark Red' (AH) stocks (13.8% of bracts). `AH' shoots developed few bract necrosis symptoms, regardless of rootstock. Calcium (Ca) concentrations were higher in whole bracts of `AH' than o

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50

Atkins, CA, and A. Pigeaire. "Application of cytokinins to flowers to increase pod set in Lupinus angustifolius L." Australian Journal of Agricultural Research 44, no. 8 (1993): 1799. http://dx.doi.org/10.1071/ar9931799.

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Exogenous application of a 2 mol m-3 buffered solution of N6 benzylaminopurine (BAP) to flowers on the main stem inflorescence of Lupinus angustifolius L, cv. Danja profoundly altered reproductive development by reducing post-anthesis abscission of flowers and small pods. The same effect of BAP was recorded for a mutant (abs-) of cv. Danja, in which organ abscission was completely absent, indicating that localized application of cytokinin enhanced reproductive development rather than reduced pedicel abscission per se in the parent line. Application to pedicel and sepals at the open flower stag

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