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1

Purba, Elida, and Kenjiro Siburian. "THE DETERMINATION OF SALINITY AND NUTRITION (NaH2PO4) PROFILE IN Nannochloropsis oculata CULTIVATION TO GAIN MAXIMUM LIPID." Reaktor 14, no. 2 (August 14, 2012): 135. http://dx.doi.org/10.14710/reaktor.14.2.135-142.

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The determination of salinity and nutrition (NaH2PO4) profile in Nannochloropsis oculata cultivationto gain maximum lipid was investigated. The purpose of this research was to determine the optimumsalinity and nutrition (NaH2PO4) to gain maximum lipid in Nannochloropsis oculata. Phosphor canbe obtained by adding NaH2PO4 and a salinity enhancement can be applied by adding NaCl to theculture. This research was run by matching each variable of salinity (33, 34, 35, and 36 ppt) to eachvariable of NaH2PO4 (5, 10 and 15 ppm) randomly. The results show that the percentage of maximumlipid in Nannochloropsis oculata was obtained at 35 ppt and 5 ppm NaH2PO4 (37.68 %). Neverthelessthe maximum lipid percentage is low and it can be caused by some factors, such as the dryingtemperature for preparing the dry microalgae to extract, the cell disruption method and the extractionsolvent used. By seeing the results, salinity can affect the total lipid in Nannochloropsis oculata. Thegreater the salinity in culture the greater the lipid content in Nannochloropsis oculata. Otherwise adifferent response was obtained by adding NaH2PO4. If NaH2PO4 concentration is greater, the lipidcontained Nannochloropsis oculata will be lower Penentuan profilsalinitas dan nutrisi (NaH2PO4) pada pembiakan Nannochloropsis oculata untuk memperoleh lipidmaksimum diteliti. Tujuan penelitian ini adalah untuk menentukan salinitas dan nutrisi (NaH2PO4)optimum untuk memperoleh lipid maksimum pada Nannochloropsis oculata. Fosfor dapat diperolehdengan menambahkan NaH2PO4 pada kultur dan peningkatan salinitas dapat dilakukan denganmenambahkan NaCl pada kultur. Penelitian ini dijalankan dengan mencocokkan setiap variabelsalinitas (33, 34, 35, dan 36 ppt) dengan setiap variabel NaH2PO4 (5, 10 dan 15 ppm) secara acak.Hasil menunjukkan bahwa persentase lipid maksimum pada Nannochloropsis oculata diperoleh padasalinitas 35 ppt dan 5 ppm NaH2PO4 (37,68 %). Walaupun demikian, persentase lipid maksimummasih tergolong rendah dan hal ini dapat disebabkan oleh beberapa faktor, seperti temperaturpengeringan yang diterapkan, metode cell disruption dan pelarut ekstraksi yang digunakan. Denganmempelajari hasil yang telah didapat, salinitas dapat mempengaruhi lipid total padaNannochloropsis oculata. Semakin tinggi salinitas pada biakan, semakin tinggi kandungan lipid padaNannochloropsis oculata. Sebaliknya didapatkan respon yang berbeda saat menambahkan NaH2PO4.Apabila konsentrasi NaH2PO4 semakin tinggi, maka lipid yang terkandung pada Nannochloropsisoculata akan menjadi lebih rendah.
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Yanuhar, Uun. "Pengaruh Pemberian Bahan Aktif Ekstrak Nannochloropsis oculata Terhadap Kadar Radikal Bebas Pada Ikan Kerapu Tikus (Cromileptes altivelis) Yang Terinfeksi Bakteri Vibrio alginolyticus [The Influence Of Supply Of Bioactive Extract Nannochloropsis oculata To Dose Of Free Radicals In The Humpback Grouper (Cromileptes altivelis) That Was Infected By Vibrio alginolyticus]." Jurnal Ilmiah Perikanan dan Kelautan 1, no. 2 (January 26, 2019): 185. http://dx.doi.org/10.20473/jipk.v1i2.11687.

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AbstractNanochloropsis oculata is one of kind green algae that overload in Indonesia ocean. The purpose of this research was to know the potency antioxidant of Nannochloropsis oculata extract as in the Humback Grouper. The research was conducted by experimently and used direct random sampling as design with 4 experiments and 3 repeats i.e control fish, fish was added Nannochloropsis oculata extract, Vibrio alginolyticus - infected fish, and Vibrio alginolyticus - infected fish that was added Nannochloropsis oculata extract. The result showed, fish that added by Nannchloropsis oculata extract made a different influence which was very clear to the decreasing of free radical in the body. Free radical (malonaldehid) in the Vibrio alginolyticus - infected fish was 4, 04 µg/100 mg which fish was added by Nannochloropsis oculata extract, the free radical level was decrease into 3, 29 µg/100 mg massa.
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3

SP Negara, Bertoka Fajar, Irfandi Irfandi, Nining Nursalim, and Nurlaila Ervina Herliany. "POTENSI Nannochloropsis oculata DAN Tetraselmis chuii SEBAGAI BAHAN BAKU BIOETANOL." Jurnal Laot Ilmu Kelautan 1, no. 2 (October 8, 2019): 23. http://dx.doi.org/10.35308/jlaot.v1i2.2315.

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Bahan bakar fosil yang semakin menipis menimbulkan permasalahan baru dalam mewujudkan ketahanan energi didunia. Bioetanol merupakan salah satu bahan bakar alternatif yang ramah lingkungan dan bersifat terbarukan. Penelitian ini bertujuan untuk memperoleh jumlah bioetanol yang dihasilkan dari fermentasi biomassa Nannochloropsis oculata dan Tetraselmis chuii. Kultur Nannochloropsis oculata dan Tetraselmis chuii selama 5 hari, hidrolisis asam dilakukan dengan menggunakan H2SO4 0,2 M dengan suhu 121 oC dan tekanan 1 atm selama 30 menit, fermentasi menggunakan Saccaromyces cereviciae selama 5 hari. hasil hidrolisis Nannochloropsis oculata memiliki kadar gula sebesar 4%, hasil fermentasi Nannochloropsis oculata mengandung etanol sebanyak 12 mL dengan konsentrasi 4%. Hasil hidrolisisyang dilakukan menghasilkan kadar gula 4%. Hasil fermentasi menghasilkan 1% bioetaniol.
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4

Ardika, Kadek, Muhdiat Muhdiat, and I. Nyoman Restiada. "PENGGUNAAN JENIS PAKAN BERBEDA PADA KULTUR ROTIFER (Brachionus rotundiformis)." Buletin Teknik Litkayasa Akuakultur 11, no. 1 (December 9, 2016): 57. http://dx.doi.org/10.15578/blta.11.1.2013.57-60.

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Keberhasilan kultur rotifer akan sangat tergantung dari jenis dan mutu pakan yang diberikan. Beberapa jenis fitoplankton yang dapat digunakan sebagai pakan rotifer adalah Nannochloropsis oculata dan Tetraselmis sp. Kegiatan ini bertujuan untuk mengetahui pengaruh penggunaan jenis pakan berbeda terhadap pertumbuhan populasi rotifer, yaitu jenis fitoplankton Nannochloropsis oculata dan Tetraselmis tetrahele, dengan dosis pemberian pakan sebagai berikut: Nannochloropsis oculata 250.000 sel/ind./hari, sedangkan Tetraselmis tetrahele 25.000 sel/ind./hari diberikan dua kal i sehari , kepadatan awal rotifer sebanyak 20 ind./mL, wadah kultur menggunakan bak fiber glas volume 30 liter sebanyak 6 buah dengan menerapkan tiga ulangan pada masing-masing perlakuan. Hasil pengamatan pertumbuhan rotifer selama 4 hari, menunjukkan bahwa kepadatan populasi rotifer tertinggi diperoleh pada pemberian jenis pakan Tetraselmis tetrahele dengan kepadatan 436 ind./mL, sedangkan pada pemberian jenis pakan Nannochloropsis oculata hanya 168 ind./mL.
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5

Oanh, Nguyen Thi Tu, Le Nguyen Thanh, Tran Huu Giap, Vu Thi Kim Oanh, Dang Diem Hong, Ngo Thi Hoai Thu, Nguyen Van Hung, Chau Van Minh, and Nguyen Thi Minh Hang. "Chemical Constituents of Nannochloropsis oculata." Chemistry of Natural Compounds 55, no. 3 (May 2019): 589–91. http://dx.doi.org/10.1007/s10600-019-02753-0.

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Boedi Setya Rahardja, Indah Permata Sari, Laksmi Sulmartiwi,. "Pengaruh Pemberian Nata De Nanno Dan Nannochloropsis oculata Terhadap Populasi Brachionus plicatilis [Effect Of Giving Nata De Nanno And Nannochloropsis oculata On Population Of Brachionus plicatilis]." Jurnal Ilmiah Perikanan dan Kelautan 5, no. 1 (January 20, 2019): 85. http://dx.doi.org/10.20473/jipk.v5i1.11430.

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Abstract This study aims to determine the effect of Nata De Nanno and Nannochloropsis oculata for Brachionus plicatilis population. The research method used was experimental with a Completely Randomized Design (CRD) with six treatments and four replications. The treatments used were: (A) Nata de Nanno 5x109 cells/L N. oculata, (B) Nata de Nanno 4x109 cells/L N. oculata, (C) Nata de Nanno 3x109 cells/L N. oculata, (D) N. oculata 5x109 cells/L, (E) N. oculata 4x109 cells/L and (F) N. oculata 3x109 cells/L. Analysis of data using variant analysis (ANOVA) and to determine the differences between treatments performed tests Distance Regression Duncan. The results showed that administration of Nata De Nanno and Nannochloropsis oculata for Brachionus plicatilis populations exert a significantly different (p<0,05) in each treatment delivery Nata De Nanno and Nannochloropsis oculata with the highest peak population of young B. plicatilis achieved on the fifth day of treatment D (N. oculata) with a concentration of 5x109 cells/L produce a population young B. plicatilis as much as 98,5 individuals/ml. The lowest peak population of young B. plicatilis on the fifth in treatment C (Nata de Nanno) with a concentration of 3x109 cells/L N. oculata 76,75 produce a population of young B. plicatilis 76,75 individuals/ml. Water quality parameters during the study was the temperature ranges between 27-31°C, a salinity of 31 ppt, pH value 7, DO (Dissolved oxygen) ranged between 5-8 mg/L, and ammonia levels ranged from 0-0.25 mg/L.
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7

Ismi, Suko. "PERKEMBANGAN POPULASI Nannochloropsis oculota PADA SUHU DAN SALINITAS YANG BERBBDA." Jurnal Penelitian Perikanan Indonesia 2, no. 2 (April 10, 2017): 68. http://dx.doi.org/10.15578/jppi.2.2.1996.68-72.

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Mengingat pentingnya bibit baru untuk kultur NNannochloropsis oculota secara massal maka perlu adanya stok murni fitoplankton ini di laboratorium. Percobaan telah dilakukan untuk mengetahui pengaruh suhu dan salinitas terhadap perkembangan populasi N. oculota. N. oculata dikultur pada botol volume satu liter dengan kepadatan awal 3 juta seUml dan intensitas cahaya1500-3000 lux dengan penyinaran selama 24 jam/hari.
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8

Shen, Y., Y. Cui, and W. Yuan. "Flocculation Optimization of Microalga Nannochloropsis oculata." Applied Biochemistry and Biotechnology 169, no. 7 (January 30, 2013): 2049–63. http://dx.doi.org/10.1007/s12010-013-0123-4.

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9

Dianursanti, Zenitha Lintang Agustin, and Dwini Normayulisa Putri. "Increased lipids production of Nannochloropsis oculata and Chlorella vulgaris for biodiesel synthesis through the optimization of growth medium composition arrangement by using bicarbonate addition." MATEC Web of Conferences 154 (2018): 01009. http://dx.doi.org/10.1051/matecconf/201815401009.

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Chlorella vulgaris and Nannochloropsis oculata are a highly potential microalgae to be used in pilot-scale of biodiesel synthesis. The essential content from these microalgae is the fatty acid of lipid which is the main target for the feed and biodiesel industries. One of the key factor in improving lipid microalgae are the arrangemment of nutrients in the growth medium. Research on the regulation of nutrients using bicarbonate (HCO3-) as an additional inorganic carbon source has been done by many studies, but the yield of lipids obtained has not been much. The aim of the study was to improve the lipid yield of Chlorella vulgaris and Nannochloropsis oculata. Variation of [HCO3-] which added to Walne medium were 25 ppm and 75 ppm, while the Walne medium without the addition of bicarbonate acts as control. The results showed that [HCO3-] 75 ppm could increase Chlorella vulgaris biomass by 0.9162 g/l with 17.0% wt, while Nannochloropsis oculata produced the greatest lipid content in [HCO3-] 25 ppm of 20.3% wt and the largest biomass on [HCO3-] 75 ppm of 1.7233 g/l.
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Sukardi, Purnama, Ferisa Wahyunika, Tjahyo Winanto, Norman Arie Prayogo, Taufan Harisam, Arif Mahdiana, and Sri Marnani. "Effect of Land Agricultural Fertilizer on Growth of Marine Single Cell Protein, Spirulina platensis, Chlorella vulgaris and Nannochloropsis." Omni-Akuatika 15, no. 2 (December 25, 2019): 69. http://dx.doi.org/10.20884/1.oa.2019.15.2.764.

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This study aimed to determine the growth rate and protein content in Nannochloropsis oculata, Chlorella vulgaris, Spirulina platensis were cultured using agricultural fertilizers. The agricultural fertilizers used were Urea, ZA and TSP. Each single cell protein was cultured using the three types of fertilizers with a ratio of Urea: ZA and TSP as follows (A) 1: 2: 1 [(10:20:10 g / L)], (B) 2: 2: 1 [(20:20:10 g / L)] and (C) 3: 2: 1 [(30:20:10 g / L)], respectively. The results showed that the best ratio of Urea, ZA and TSP fertilizers to growth of Spirulina, Nannochloropsis oculata and Chlorella vulgaris was C [30:20:10 (g / L)] treatment. However, phyto-protein content in Nannochlorophsis differed significantly between fertilization treatments, C fertilization yielded the highest protein content (28.75±0.05%), when compared with A (25.13±0.01%) and B (25.14±0.02%), respectively. In Chlorella vulgaris, all fertilization treatments showed very significant differences, B fertilization (28.24%±00.006) yielded the highest phyto-protein content, if compared to A (23.63% ± 0.003) and C (19.74% ±0.006 ), respectively. All fertilization treatments showed very significant differences (P <0.05) on Spirulina platensis. The highest content of phyto-protein (62.68 + 0.05%) was present in treatment C, when compared to A (52.18 + 0.05%) and B (62.37 + 0.01%) treatments, respectively.
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Park, Hyun-Jin, Young-Hwa Kim, and Jae-Hwa Lee. "Effect of Alginate on the Growth of Nannochloropsis oculata NIES-2145." KSBB Journal 26, no. 3 (June 30, 2011): 206–10. http://dx.doi.org/10.7841/ksbbj.2011.26.3.206.

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12

Kurnia, Agus Rivaldy, Sakha Abdussalam, and Elida Purba. "Batas Toleransi Laju Alir dari Mikroalga (Nannochloropsis Oculata dan Botrycoccus Braunii) dengan Konsentrasi CO2 Konstan dalam Fotobioreaktor." Inovasi Pembangunan : Jurnal Kelitbangan 5, no. 02 (August 1, 2017): 146–55. http://dx.doi.org/10.35450/jip.v5i02.42.

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Penelitian ini dilakukan untuk mengabsorbsi kandungan CO2 di udara dengan memanfaatkan mikroalga sebagai agen penyerap CO2. Dalam melakukan penelitian, digunakan dua jenis mikroalga yaitu Nannochloropsis oculata dan Botrycoccus braunii. Tujuan dari penelitian ini untuk mendapatkan batas toleransi laju alir dengan konsentrasi CO2 yang konstan didalam fotobioreaktor serta membandingkan kedua mikroalga yang digunakan dari data penyerapan CO2 dan pertumbuhan biomassa. Pada penelitian ini digunakan variasi laju alir umpan yaitu 1 l/menit, 1,5 l/menit, dan 2 l/menit dengan konsentrasi CO2 tetap sebesar 33 %. Penelitian dilakukan dengan menggunakan volume kultur sebanyak 4 l (1 l mikroalga dan 3 l air laut) selama 6 hari kultur. Hasil penelitian menunjukkan bahwa laju alir optimum untuk Nannochloropsis oculata adalah pada laju alir 2 l/menit dapat menghasilkan persentase penyerapan sebesar 71,54 %. Sedangkan laju alir optimum untuk Botrycoccus braunii adalah 1 l/menit menghasilkan persentase penyerapan sebesar 70,16 % . Dari data hasil penelitian tersebut didapatkan titik toleransi maksimum laju alir umpan untuk Botrycoccus braunii adalah 1 l/menit dengan konsentrasi 33 %, sedangkan untuk Nannochloropsis oculata belum didapatkan titik toleransi laju alir maksimum karena pada variasi laju alir 2 l/menit dengan konsentrasi CO233 % masih memiliki kemampuan yang baik dalam proses biofiksasi CO2. Oleh karena itu perlu diberikan variasi laju alir yang lebih besar lagi untuk mikroalga jenis Nannochloropsis oculataagar didapatkan titik toleransi laju alir.
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Qiu, Changyang, Yongjin He, Zicheng Huang, Weisang Qiu, Jian Huang, Mingzi Wang, and Bilian Chen. "Biosafety evaluation of Nannochloropsis oculata and Schizochytrium sp. oils as novel human milk fat substitutes." Food & Function 12, no. 7 (2021): 2972–84. http://dx.doi.org/10.1039/d0fo03000g.

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14

Kagan, Michael L., and Ray A. Matulka. "Safety assessment of the microalgae Nannochloropsis oculata." Toxicology Reports 2 (2015): 617–23. http://dx.doi.org/10.1016/j.toxrep.2015.03.008.

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15

Huertas, I. Emma, and Luis M. Lubián. "Comparative study of dissolved inorganic carbon utilization and photosynthetic responses in Nannochloris (Chlorophyceae) and Nannochloropsis (Eustigmatophyceae) species." Canadian Journal of Botany 76, no. 6 (June 1, 1998): 1104–8. http://dx.doi.org/10.1139/b98-068.

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Four species of marine microalgae with similar morphology and life cycle, namely Nannochloris atomus Butcher, Nannochloris maculata Butcher, Nannochloropsis gaditana Lubian, and Nannochloropsis oculata (Droop) Hibberd, have been examined with respect to their affinity for different sources of dissolved inorganic carbon. External carbonic anhydrase activity was not found in any of these species, but the cell affinity for dissolved inorganic carbon (DIC) in Nannochloris species was affected by the inhibitor acetazolamide at a concentration of 400 µM. Measurement of photosynthetic rates and CO2 compensation points at different pH values showed that the Nannochloris species had a greater capacity for CO2 rather than HCO3- utilization. In contrast, the observed rates of photosynthetic oxygen evolution in Nannochloropsis species were greater than could be accounted for by the theoretical rate of CO2 supply from the spontaneous dehydration of bicarbonate in the external medium. This indicates that these algae were able to transport bicarbonate across the plasmalemma. Furthermore, the K0.5 (DIC) value at acidic pH showed that Nannochloropsis oculata could also use CO2 as an exogenous carbon source for photosynthesis. Although the species of marine phytoplankton used in this study possess similar morphological characteristics and life cycle, there exist many differences in the mode of inorganic carbon utilization between these microalgae.Key words: Nannochloris, Nannochloropsis, inorganic carbon utilization, bicarbonate transport, CO2 compensation point, photosynthesis.
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Ismi, Suko, and Wardoyo Wardoyo. "PENGGUNAAN Nannochloropsis oculato AWETAN DAN YANG DIPERKAYA VITAMIN 812 UNTUK KULTUR ROTIFER." Jurnal Penelitian Perikanan Indonesia 3, no. 4 (April 7, 2017): 67. http://dx.doi.org/10.15578/jppi.3.4.1997.67-72.

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Percobaan ini dilakukan untuk mengetahui pengaruh pemberian Nannochloropsis oculata yang dipekatkan kemudian disimpan dalam bentuk dingin dan beku serta telah diperkaya dengan vitamin B12, terhadap pertumbuhan populasi rotifer, Brachionus plicatilis.
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Ghafari, Mohammad Aulia, Indyaswan Tegar Suryaningtyas, Defri Yona, Serly Sapulete, Nurhalis Tarmin, La Ali, Ramli Marzuki, and Ratih Pangestuti. "Effects of F/2 media modification on growth and antioxidant activity of microalga Nannochloropsis oculata." Marine Research in Indonesia 45, no. 1 (July 27, 2020): 33–38. http://dx.doi.org/10.14203/mri.v45i1.560.

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Nannochloropsis oculata is a common microalga that can be found in marine water. One of the most commonly used media for microalga culture is F/2 media; however, the optimum F/2 media composition for N. oculata growth is rarely informed. Therefore, the aims of this study are to determine the effect of F/2 medium modification on the growth of N. oculata and the antioxidant activity of N. oculata. This study consists of several procedures including; cultivation, growth observation (cell density and biomass), extraction, and antioxidant activity of N. oculata extracts. The highest growth (cell density and biomass) of N. oculata was found in media F/2 + 0.5 ml, while the lowest was in media F/2 - 0.5 ml. The strongest antioxidant activity of N. oculata was found in media F/2 + 1 ml, and the lowest was in the media F/2 - 0.5 ml. Media modification gave different results on cell density, biomass, and antioxidant activity of microalgae N. oculata.
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Jeong, U.-Cheol, Jong-Cheol Han, Byeong-Dae Choi, and Seok-Joong Kang. "Lipid and Fatty Acid Composition in Nannochloropsis oculata Cultured in Varying Salinities." Korean Journal of Fisheries and Aquatic Sciences 46, no. 3 (June 30, 2013): 252–58. http://dx.doi.org/10.5657/kfas.2013.0252.

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Archibeque, S., A. Ettinger, and B. Willson. "Nannochloropsis oculata as a source for animal feed." Acta Agronomica Hungarica 57, no. 2 (June 1, 2009): 245–48. http://dx.doi.org/10.1556/aagr.57.2009.2.16.

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Sidharthan, M., Kim Shin Young, Lee Hoe Woul, Park Kwan Soon, and Hyun Woung Shin. "TBT toxicity on the marine microalga Nannochloropsis oculata." Marine Pollution Bulletin 45, no. 1-12 (September 2002): 177–80. http://dx.doi.org/10.1016/s0025-326x(01)00283-1.

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21

Poncet, Jean-Marc, and Beno^ıt Véron. "Cryopreservation of the unicellular marine alga,Nannochloropsis oculata." Biotechnology Letters 25, no. 23 (December 2003): 2017–22. http://dx.doi.org/10.1023/b:bile.0000004395.04116.45.

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Gwo, Jin-Chywan, Ju-Yu Chiu, Chin-Cheng Chou, and Hsien-Yu Cheng. "Cryopreservation of a marine microalga, Nannochloropsis oculata (Eustigmatophyceae)." Cryobiology 50, no. 3 (June 2005): 338–43. http://dx.doi.org/10.1016/j.cryobiol.2005.02.001.

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23

Shen, Y., C. Chen, W. Chen, and X. Xu. "Attached culture of Nannochloropsis oculata for lipid production." Bioprocess and Biosystems Engineering 37, no. 9 (February 21, 2014): 1743–48. http://dx.doi.org/10.1007/s00449-014-1147-z.

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Restiada, I. Nyoman, Feri Priatna, Muhdiat Muhdiat, and Kadek Ardika. "PENGGUNAAN FORMULA PUPUK YANG BERBEDA TERHADAP KANDUNGAN AMONIAK (NH3) Nannochloropsis oculata PADA KULTUR SKALA MASSAL." Buletin Teknik Litkayasa Akuakultur 14, no. 2 (November 15, 2016): 87. http://dx.doi.org/10.15578/blta.14.2.2016.87-90.

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Nannochloropsis oculata adalah alga bersel satu yang termasuk dalam kelas Eustigmatophyceae. N. oculata berperan penting dalam kegiatan pembenihan khususnya larva ikan. Namun penggunaan N. oculata sebagai green water dapat menyebabkan dampak negatif terhadap media pemeliharaan larva, satu di antaranya adalah meningkatkan kandungan nitrogen (N) dalam bentuk NH3 (amoniak). Keberadaan NH3 pada media kultur disebabkan oleh penggunaan pupuk kimia sebagai nutrien dalam proses berkembang biak N. oculata. Salah satu cara untuk mengurangi kandungan NH3 pada media pemeliharaan larva bisa dilakukan dengan cara mengatur penggunaan formula pupuk pada media kultur. Tujuan dari percobaan ini adalah untuk mendapatkan informasi tentang pengaruh penggunaan formula pupuk terhadap kandungan NH3 (amoniak) pada media kultur N. oculata. Kegiatan ini menggunakan empat unit bak beton berbentuk persegi empat dengan ukuran 4 m x 2,5 m x 1 m dan beberapa jenis pupuk pertanian. Kultur massal N. oculata menggunakan persentase formula pupuk 100%, 75%, 50%, dan 25%. Hasil pengamatan menunjukkan bahwa dengan formula pupuk 25% menghasilkan kandungan NH3 yang paling rendah dibandingkan formula pupuk yang lain
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Suprayudi, Muhammad Agus. "Effect of Kind and Dosage of Enrichment Materials on the Nutritional Quality of Rotifers Especially n3-HUFA." Jurnal Akuakultur Indonesia 2, no. 1 (May 1, 2007): 21. http://dx.doi.org/10.19027/jai.2.21-25.

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<p>This experiment was conducted to evaluate the quality of rotifer enriched with four kinds of enrichement materials from the stand point of essensial fatty acids. Rotifer was enriched at 24 - 25<sup>o</sup>C for 18 hours at a density of 1000 ind/ml. Rotifers were treated by four kinds of enrichment materials such as oleic acid (R-OA), two different density of <em>Nannochloropsis oculata</em>, (4x10<sup>7</sup> and 16x10<sup>7</sup> cell/ml; R-N18 and R-N42) two different levels of eicosapentaenoic acids (EPA) triglyceride type (EPA-TAG) (20 and 40 ml/ml; R-E20, R-E40) and two different level of EPA ethyl ester (EPA-EE) (R-EE25 and R-EE50%) respectively. Rotifers enriched with <em>Nannochloropsis</em> <em>oculata</em> and EPA-EE type have a similar profile of essensial fatty acid especially on n3-HUFA that dominated by EPA, while DHA was in a trace amount or not detected. In addition <em>Nannochloropsis</em> <em>oculata</em> as an enrichment material showed the highest population density of rotifers during enrichment periods. Rotifer enriched with EPA-TAG has a more complete of essential fatty acid profile compared to other enrichment materials due to their contained both of EPA and DHA. We conclude that rotifer enriched with EPA-TAG as enrichment material showed the best nutritional quality of rotifers from the stand point of essential fatty acid.</p> <p>Key words : Rotifers, enrichment, eicosapentaenoic acid, docosaheksaenoic acid, n3-HUFA</p> <h2> </h2> <h2>ABSTRAK</h2> <p>Penelitian ini dilakukan untuk mengetahui profil asam lemak rotifera yang diperkaya dengan berbagai macam jenis dan dosis pengkaya. Rotifera dengan kepadatan 1000 ind./ml diperkaya dengan bebagai bahan pengkaya seperti asam oleat (R-OA); <em>Nannochloropsis oculata </em>dengan kepadatan 4x10<sup>7</sup> dan 16x10<sup>7</sup> sel/ml (R-N18 dan R-N42); eicosapentaenoic acid (EPA) tipe triglicerida (EPA-TAG) dengan dosis 20 dan 40 μl (R-E20 dan R-E40) dan EPA tipe ethyl esther (EPA-EE) dengan dosis 25 dan 50 μl per liter (R-EE25 and R-EE50%). Rotifera diperkaya selama 18 jam pada suhu 24-25<sup>o</sup>C. Rotifera yang diperkaya dengan <em>Nannochloropsis oculata</em> serta EPA-EE memiliki kesamaan profil asam lemak terutama pada incorporasi EPA, sedangkan DHA terkandung pada jumlah yang kecil atau tidak terdeteksi. Adapun rotifera yang diperkaya dengan EPA-TAG memiliki profil asam lemak yang lebih lengkap terutama ditinjau dari kandunga EPA dan docosahexaenoic acid (DHA) nya. Selanjutnya rotifera yang diperkaya dengan <em>Nannochloropsis</em> <em>oculata</em> memiliki populasi yang tertinggi dibanding perlakuan lainnya. Dari penelitian ini dapat disimpulkan bahwa pengkayaan dengan EPA-TAG memiliki kualitas rotifera yang terbaik ditinjau dari sisi kelengkapan kandungan asam lemaknya.</p> <p>Kata kunci : Kualitas rotifera, pengkayaan, asam eikosapentaenoik, asam dokosaheksaenoik, n3-HUFA</p>
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DURMAZ, Yaşar, and Gökhun Çağatay ERBİL. "Effects of myo-inositol concentration on growth and pigments of Nannochloropsis oculata culture." Ege Journal of Fisheries and Aquatic Sciences 37, no. 2 (June 15, 2020): 195–99. http://dx.doi.org/10.12714/egejfas.37.2.11.

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Inositols are used as growth promoting agents over plants. But microalgae are different from higher plant especially photosynthetic efficiency and productivity. According to the results of this study, myo-inositol addition to the culture medium of Nannochloropsis oculata provides higher cell densities. 100 mg L-1 myo-inositol added experimental group was reached to 1.42 fold cell mL-1, while the 500 mg L-1 myo-inositol added group was reached to 1.28 fold cell mL-1 than the control group. Mean chlorophyll a per cell amounts were calculated for experimental groups and control groups as 0.052 pg cell-1 and 0.053 pg cell-1, respectively. Mean total carotene per cell amounts were calculated for all groups as 0.016 pg cell-1. These results show that no difference was occurred between all groups by chlorophyll a and total carotene amounts per cell. This study shows that myo-inositol use in microalgae production may provide higher yields.
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Sofiyah, Evi Siti, and I. Wayan Koko Suryawan. "Cultivation of Spirulina platensis and Nannochloropsis oculata for nutrient removal from municipal wastewater." Rekayasa 14, no. 1 (March 19, 2021): 93–97. http://dx.doi.org/10.21107/rekayasa.v14i1.8882.

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Domestic wastewater contains a high average nutrient ammonia-N (NH3-N) and total phosphate (PO43--P). This nutrient content has the potential to cause eutropication in water bodies. To prevent this eutropication, it is necessary to treat domestic wastewater. Currently, processing technology is needed that is useful for improving the quality of processed wastewater and a small amount of byproduct. One of these technologies is processing with a microalgae system, where the algae can be used to become biodiesel. Two types of microalgae that have the potential to produce biodiesel are Spirulina platensis and Nannochloropsis oculate. The cultivation of the two types of microalgae was carried out in the domestic wastewater media of Jakarta City by providing 24-hour lighting with UV-A and UV-B. The specific growth rates of Spirulina platensis and Nannochloropsis oculate were not much different, namely 0.0279 h-1 and 0.0282 h-1. The microalgae Spirulina platensis and Nannochloropsis oculate respectively reduced NH3-N nutrients by 82% and 80%, while PO43-P was 65.2% and 63.7%. The pH value during processing shows in the normal pH range. Total dissolved solids (TDS) in the processing process also decreased in a span of 48 hours.
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Zainal Abidin, Aisamuddin Ardi, Chotika Yokthongwattana, and Zetty Norhana Balia. "Carotenogenesis in Nannochloropsis oculata under Oxidative and Salinity Stress." Sains Malaysiana 50, no. 2 (February 28, 2021): 327–37. http://dx.doi.org/10.17576/jsm-2021-5002-05.

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Nannochloropsis oculata is a unicellular microalgae which is vastly found throughout the environment and have been widely studied due to its high productivity of secondary metabolites and oil content. It is majorly cultured in the aquaculture sector as fish feed and for industries for its polyunsaturated fatty acids. This work aims to study the impact of salinity and oxidative stress on the expression of carotenoid biosynthesis genes and the accumulation of their products in N. oculata via qPCR and HPLC analyses. Three genes responsible for production of high value carotenoids namely lycopene beta-cyclase (CrTL-B/LCYB), beta-carotene oxygenase (CrTO)and beta-carotene hydroxylase (CrTR) under different stresses and time points were identified and quantified, and the amount of their products namely β-carotene, zeaxanthin, canthaxanthin, and astaxanthin was measured. N. oculata was treated with different concentrations of Cu2+ ion (1, 2, and 5 ppm) and NaCl (50, 150, 250 mM) which resembles conditions of oxidative and salinity stress, respectively. RNA and carotenoids extraction, RT-PCR, qPCR and HPLC was carried out in order to identify the correlation of carotenogenesis genes expression with carotenoids production. Under exposure of both treatments, the carotenoids biosynthesis genes were upregulated up to 6-fold compared to control and targeted carotenoids were overexpressed up to 7-fold. Results from this study gave insights which are beneficial in understanding microalgae’s responses towards abiotic stress via the synthesis of carotenoids.
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Arihanda, Dea Davita Putri, Suryono Suryono, and Gunawan Widi Santosa. "Kadar Total Lipid Mikroalga Nannochloropsis oculata Hibberd, 1981 (Eustigmatophyceae : Eustigmataceae) Berdasarkan Perbedaan Salinitas dan Intensitas Cahaya." Journal of Marine Research 8, no. 3 (August 29, 2019): 229–36. http://dx.doi.org/10.14710/jmr.v8i3.25263.

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Nannochloropsis oculata merupakan alga yang memiliki kadar lipid tinggi dan mudah dibudidayakan karena hanya bantuan cahaya matahari, karbon dioksida dan air laut mampu berkembang biak dengan baik. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan salinitas dan intensitas cahaya terhadap pertumbuhan dan kadar total lipid pada mikroalga N. oculata. Penelitian ini dilaksanakan secara laboratoris, dengan Rancangan Faktorial. Perlakuan yang diuji cobakan yaitu A1( Salinitas 33 ‰), A2 (Salinitas 31 ‰), dan A3 (Salinitas 35 ‰), serta B1 (Intensitas Cahaya 500 lux), B2 (Intensitas Cahaya 1500 lux), B3 (Intensitas Cahaya 3000 lux), yang diperbandingkan dengan Perlakuan Kontrol. Setiap perlakuan dilakukan pengulangan sebanyak 3 kali. Hasil analisis pertumbuhan mikroalga N. oculata dan kandungan lipid dilakukan dengan Analisis Sidik Ragam dan dilakukan uji lanjutan Analisis Duncan Multiple Range Test dilaksanakan untuk mengetahui perbedaan perlakuan. Hasil penelitian menunjukkan bahwa pertumbuhan mikroalga N. oculata tertinggi dicapai oleh perlakuan A1B3 (Salinitas 33 ‰ dan Intensitas Cahaya 3000 lux) sebesar 19,927x103±5,454x103 sel/mLdan A3B1 (Salinitas 35 ‰ dan Intensitas Cahaya 500 lux) sebesar 20,779x103 ± 5,874x103 sel/mL. Pola pertumbuhan mikroalga N. oculata untuk semua perlakuan berbentuk kuadratik. Kandungan lipid mikroalga N. oculata tertinggi dicapai oleh perlakuan A3B1 (Salinitas 35 ‰ dan Intensitas Cahaya 500 lux) dengan nilai sebesar 66,5767±1,5257 mg/g dan terendah pada perlakuan A3B3 (Salinitas 35 ‰ dan Intensitas Cahaya 3000 lux) dengan nilai sebesar 38,2010±3,1507 mg/g.Nannochloropsis oculata is one of algae that has high lipid content compared to other algae and is easily cultivated because only the help of sunlight, carbon dioxide and sea water can reproduce well. This aims of the study was to determine the effect of varions salinity and light intensity on the growth and total lipid contents of microalgae N. oculata. The method used was an experimental laboratory method with a factorial design. The treatments applied were A1 (Salinity 33 ‰), A2 (Salinity 31 ‰), and A3 (Salinity 35 ‰), and B1 (Light Intensity 500 lux), B2 (Light Intensity 1500 lux), B3 (Light Intensity 3000 lux), which compare to the Control. The Interaction Treatments were applied on three replicates. The microalga N. oculata and Lipid Contents were analysed using Factorial Approach on Variance Analysis. The Duncan Multiple Range Test was applied to show the treatment which influence the difference result. The highest microalga N. oculata growth showed by the treatment of A1B3 (Salinity 33 ‰ and Light Intensity 3000 lux) with the number 19,927x103 cells/mL ± 5,454x103 cells/mL and A3B1(Salinity 35 ‰ and Light Intensity 500 lux) with the number 20,779x10 cells/mL ± 5,874x103 cells/mL. The growth model of N. oculata for all treatments showed a model of quadratic.The highest lipid content of N. oculata showed by the treatment ofA3B1 (Salinity 35 ‰ and Light Intensity 500 lux) with the number 66,5767±1,5257 mg/g and the lowest on A3B3 (Salinity 35 ‰ and Light Intensity 3000 lux) with the number38,2010±3,1507 mg/g.
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Sales, Rafael, and Lília Pereira de Souza-Santos. "Production of concentrated inocula from the microalgae Nannochloropsis oculata." Aquaculture International 28, no. 4 (April 10, 2020): 1609–20. http://dx.doi.org/10.1007/s10499-020-00546-9.

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Abdul Manan, Indah Permata Sari,. "Pola Pertumbuhan Nannochloropsis oculata Pada Kultur Skala Laboratorium, Intermediet, Dan Massal [Patterns Growth Of Nannochloropsis oculata In Culture Scale Laboratory, Intermediate, And Bulk]." Jurnal Ilmiah Perikanan dan Kelautan 4, no. 2 (January 23, 2019): 123. http://dx.doi.org/10.20473/jipk.v4i2.11562.

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Abstract One type of phytoplankton that are used in marine fish hatchery activities namely Nannochloropsis oculata. The purpose of study case is to know patterns growth of N. oculata in culture scale laboratory, intermediate, and bulk. Study was held in Situbondo Brackishwater Aquaculture Center of East Java in January-February 2011. Study case methods used is descriptive method. N. oculata culture techniques performed by multilevel methods, beginning with the isolation of phytoplankton in the media for/liquid, culture in test tubes 10 ml and elenmeyer 50-100 ml, culture on the bottle 100-1000 ml, culture in karboy of 20 liters, intermediate scale culture 100 - 1000 liters and a mass-scale culture with a volume of more than 1000 liters. Laboratory-scale use Walne for fertilizer. Intermediate-scale use of fertilizers FeCl3 1-2 ppm, 10 ppm EDTA, Na2HPO4 10-15 ppm, 100-150 ppm KNO3, and Tracemetals/vitmix 5 ml/m3, mass-scale culture use of fertilizers FeCl3 1 ppm, 5 ppm EDTA, TSP 20 ppm, ZA 40 ppm and 50 ppm urea. Water quality measurement results obtained by laboratory scale temperature is 23 - 25°C, intermediates and mass scale is 30°C, pH in the culture laboratory scale and intermediate is 7.7 to 7.8, mass culture pH is 7.9, salinity laboratory scale 29-30 ppt, intermediates and mass scale 30 ppt. During mass culture of N. oculata showed that the growth pattern in accordance with the general pattern of phytoplankton growth. Mass-scale culture produce 1504 x 104 cells/ml for six days.
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SILVA, José William Alves, Glacio Souza ARAÚJO, Anderson Alan da Cruz COELHO, João Henrique Cavalcante BEZERRA, Dilliani Naiane Mascena LOPES, and Wladimir Ronald Lobo FARIAS. "Effect of nitrate depletion on lipid accumulation by the marine microalga Nannochloropsis oculata." Boletim do Instituto de Pesca 41, especial (September 30, 2015): 811–16. http://dx.doi.org/10.20950/1678-2305.2015v41nep811.

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Puspita, Lani. "Struktur Komunitas Plankton Pada Muara Sungai Enam, Kabupaten Bintan, Provinsi Kepulauan Riau." SIMBIOSA 7, no. 1 (July 30, 2018): 55. http://dx.doi.org/10.33373/sim-bio.v7i1.1314.

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Kelurahan Sei Enam, Kecamatan Bintan Timur merupakan salah satu daerah pertambangan bauksit di Kabupaten Bintan. Salah satu dampak dari kegiatan pertambangan bauksit adalah penurunan kualitas air di badan air penerima karena adanya limbah tailing (khususnya apabila tailing tidak dikelola dengan baik). Penelitian ini bertujuan untuk mengetahui struktur komunitas fitoplankton dan zooplankton di Muara Sungai Enam, yang meliputi jumlah taxa, komposisi jenis, keanekaragaman jenis, keseragaman jenis, dan dominansi jenis. Hasil penelitian ini diharapkan dapat dijadikan informasi bermanfaat untuk mengambarkan kondisi lingkungan perairan di Muara Sungai Enam Kabupaten Bintan. Berdasarkan hasil penelitian, dapat disimpulkan bahwa keanekaragaman jenis plankton di Muara Sungai Enam tergolong rendah. Hal ini karena muara sungai merupakan daerah peralihan (ekoton) antara ekosistem darat dengan ekosistem laut. Salinitas di perairan ekosistem ekoton ini diprediksi sangat bervariasi, kondisi ini membuat hanya spesies-spesies dengan daya toleransi cukup tinggi saja yang dapat hidup daerah ini. Walaupun demikian produktivitasnya cukup baik karena fitoplankton yang mendominasi adalah Nannochloropsis oculata. Nannochloropsis oculata pada perairan berperan sebagai pakan alami larva-larva ikan. Sejalan dengan fitoplankton, zooplankton yang merupakan herbivora primer juga memiliki keanekaragaman jenis yang rendah.
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Galina, Daiana, Vinícius Molini Benedito, Rodrigo Randow de Freitas, and Paulo Sérgio da Silva Porto. "Análise da influência da temperatura e do tempo na transesterificação direta da Nannochloropsis Oculata para produção de biodiesel." Research, Society and Development 9, no. 7 (May 30, 2020): e655974648. http://dx.doi.org/10.33448/rsd-v9i7.4648.

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O biodiesel possui um grande potencial para substituir o diesel de petróleo. Apesar de sua produção a partir de microalgas gerar várias vantagens ambientais (consumo de dióxido de carbono atmosférico e efluentes podem ser usados como fonte de nutrientes), ele apresenta dificuldades para ser implementado na matriz energética de diversos países devido ao seu elevado custo associado às muitas etapas do processo de produção. Deste modo, o presente trabalho tem como objetivo principal analisar a influência das variáveis temperatura e tempo na produção do biodiesel via transesterificação direta de biomassa de microalgas da espécie Nannochloropsis oculata, identificando as condições que proporcionam rendimento máximo do extrato rico em biodiesel. Por meio do método experimental realizado foi possível cultivar microalgas da espécie Nannochloropsis oculata e, a partir da biomassa obtida, produzir biodiesel através da reação de transesterificação direta. A formação de biodiesel foi verificada por meio da análise de FTIR que apontou a presença de ésteres no produto final, com os pontos de rendimento máximo sendo obtidos nas condições 70 ºC e 60 min e a 110 ºC e 60 min.
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Hsu, Wei-Hsuan, Yin-Hsuan Chien, and Hung-Yin Tsai. "Experimental and Modeling Analysis of the Cell-Wall Fracture of Nannochloropsis Oculata." Journal of Mechanics 36, no. 6 (June 29, 2020): 789–97. http://dx.doi.org/10.1017/jmech.2020.22.

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ABSTRACTIn this study, a spherical indenter mounted on an atomic force microscope (AFM) was used to compress a Nannochloropsis oculata (N. oculata) cell on a poly-l-lysine coated slide. A mathematical model of the cell, which was derived by considering a fluid-filled spherical shell with axisymmetric compression between a sphere and an infinite flat plate, is proposed. In the construction of this mathematical model, the spherical shell was assumed to be a homogenous, isotropic, and elastic material. Thin-film theory was applicable to the spherical shell because the thickness of the shell was nearly negligible compared with its diameter. The governing equations of the contact and noncontact regions were converted from a boundary condition problem to an initial value problem. Then, the fourth-order Runge–Kutta method was applied to solve the transformed governing equations. The force curve obtained from the compression experiment was compared with the theoretical results derived from the proposed model. Furthermore, the numerical solution of the proposed model was verified to be consistent with the experimental data. The mechanical properties of cell walls were confirmed by applying the least square error method. Subsequently, the contact radius, inner pressure and tension distribution of the cell wall could be determined using the proposed model. The models proposed in other studies are suitable for analyzing the compression characteristics of cells whose size is of the order of tens of micrometers and millimeters. By contrast, the model proposed in this study can analyze the compression characteristics of N. oculata, which is only a few micrometers in diameter. Furthermore, a force curve that accurately describes the deformation behavior of N. oculata under strain levels of 25% was established.
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Mamdouh, Alzahraa, Eman Zahran, Fatma Mohamed, and Viola Zaki. "Protective effect of Nannochloropsis Oculata against mercuric-induced histopathological alterations in the kidney of Nile tilapia." Mansoura Veterinary Medical Journal 21, no. 3 (September 29, 2020): 67–73. http://dx.doi.org/10.35943/mvmj.2020.21.312.

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Objective: The present study was designed to evaluate the toxic effect of sublethal concentration of mercuric chloride (0.3 mg/L HgCl2) on histopathological lesions in the kidney of Nile tilapia (O. niloticus) and the protective effect of microalgae, Nannochloropsis oculata (N. oculata) against the induced tissue alterations for 3 weeks. Design: Randomized controlled study Animals: Nile tilapia Procedures: Fish were randomly assigned to 4 groups, group1: control (basal diet), group 2 (Hg/ exposed to HgCl2 at a dose of 0.3 mg/L (1/4 of LC50), and fed basal diet), group 3: (Hg+N5, similar to group2, but fed diet supplemented with N. oculata 5% and group 4 (Hg+N10, similar to group2, but fed diet supplemented with N. oculata 10 %. Two fish from each aquarium tank (6 fish/group) were sampled at weeks 1, 2, and 3 of the experiment. The posterior kidney was dried out in a graded ethanol series and then embedded in paraffin. Each block of tissue was cut into serial sections (5 μm thick) and stained with hematoxylin and eosin (H&E). Results: Histopathological alterations were induced following mercuric exposure in a time-dependent manner. The kidney showed congestion, hemosiderosis, and hemorrhage with vacuolated tubular epithelium, hyaline droplet degeneration, and necrosis of the tubular epithelium. Supplementation with N. oculata, particularly at 10 %, succeeded in alleviating the histopathological induced lesions in the kidney. Conclusion and clinical relevance: Our findings demonstrate that HgCl2 has nephrotoxic properties that led to severe histopathological alterations in the kidney of Nile tilapia, while dietary supplementation with N. oculata was able to alleviate the induced kidney alterations.
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Cavonius, L. R., E. Albers, and I. Undeland. "In vitro bioaccessibility of proteins and lipids of pH-shift processed Nannochloropsis oculata microalga." Food & Function 7, no. 4 (2016): 2016–24. http://dx.doi.org/10.1039/c5fo01144b.

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38

Kholif, Ahmed E., Gouda A. Gouda, and Hatem A. Hamdon. "Performance and Milk Composition of Nubian Goats as Affected by Increasing Level of Nannochloropsis oculata Microalgae." Animals 10, no. 12 (December 21, 2020): 2453. http://dx.doi.org/10.3390/ani10122453.

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Fat supplementation affects the lactational performance of goats and dramatically changes milk nutritive value. In the present experiment, two levels of Nannochloropsis oculata microalgae, a natural source of rumen-protected eicosapentaenoic acid (EPA), were studied in the diet of Nubian goats. Using quintuplicated 3 × 3 Latin square design, fifteen lactating goats, (14 ± 2 months old and 33.0 ± 1.3 kg) after kidding, were randomly assigned into three treatments in an 84-d assay. Goats were offered a basal diet comprising berseem clover, wheat straw and concentrates in 3:2:5, respectively, (control treatment-no supplementation). The other two treatments were supplemented with N. oculata microalgae at 5 g (NOM5 treatment) or 10 g (NOM10 treatment)/doe/d. Without affecting intake, treatments improved (p < 0.01) nutrient digestibility. Supplementations had no effect on ruminal pH and ammonia-nitrogen, however, NOM5 and NOM10 linearly improved (p < 0.05) total volatile fatty acids and propionic acids. N. oculata supplementation linearly increased (p < 0.01) milk yield and lactose content. Supplementation reduced atherogenic index (p = 0.004) and enhanced the concentrations of unsaturated fatty acids and C20:5n3 (EPA). Conclusively, feeding Nubian goats on diet supplemented with N. oculata at 5 and 10 g improved milk production and the nutritive value. No improvements in the performance were observed when N. oculata dose was increased from 5 g to 10 g/doe; thus, 5 g dose is recommended for use.
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Chu, Feng-Jen, Terng-Jou Wan, His Chen, Chih-Hung Wu, and Po-Min Kao. "Magnetophoretic Harvesting of Nannochloropsis oculata Using Iron Oxide Immobilized Beads." Water 12, no. 1 (January 15, 2020): 236. http://dx.doi.org/10.3390/w12010236.

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In this work, the harvesting of Nannochloropsis oculata microalgae through the use of nanosized Fe3O4 immobilized in polyvinyl alcohol (PVA)/sodium alginate (SA) as a flocculant (Fe3O4/PS) is investigated. Using the Fe3O4/PS immobilized beads could reduce the amount of soluble ferrous ions (Fe2+) released from naked Fe3O4 in acid treatment, leading to easy recovery. The characterization was performed under different dosages and pH values of Fe3O4/PS. The results show that the Fe3O4/PS, when applied to the algae culture (500 mg dry cell weight/L), achieves a 96% harvesting efficiency under conditions of a pH of 4 with 200 mT magnetic field intensity. Fe3O4/PS can be directly reused without adjusting the pH value. The recycled Fe3O4/PS shows stability in terms of its surface properties, maintaining more than 80% harvesting efficiency after five recycles. Magnetophoretic harvesting, using immobilized magnetic iron oxide as a particle-based flocculant, is a potential method to reduce challenges related to the cost-effective microalgae-harvesting method.
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Lima, Kárita Fernanda Fontes, Paulo Sergio da Silva Porto, and Rodrigo Randow de Freitas. "Extraction of bio-oil via wet based from Nannochloropsis oculata." International Journal of Advanced Engineering Research and Science 6, no. 7 (2019): 116–28. http://dx.doi.org/10.22161/ijaers.6715.

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41

Crampon, Christelle, Adil Mouahid, Sid-Ali Amine Toudji, Olivier Lépine, and Elisabeth Badens. "Influence of pretreatment on supercritical CO2 extraction from Nannochloropsis oculata." Journal of Supercritical Fluids 79 (July 2013): 337–44. http://dx.doi.org/10.1016/j.supflu.2012.12.022.

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42

Heng, Ri-Liang, and Laurent Pilon. "Time-dependent radiation characteristics of Nannochloropsis oculata during batch culture." Journal of Quantitative Spectroscopy and Radiative Transfer 144 (September 2014): 154–63. http://dx.doi.org/10.1016/j.jqsrt.2014.04.008.

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Hashimoto, Haruki. "Mitochondrion-dividing Ring in an Alga Nannochloropsis oculata (Eustigmatophyceae, Heterokonta)." CYTOLOGIA 69, no. 3 (2004): 323–26. http://dx.doi.org/10.1508/cytologia.69.323.

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Sukarni, Sudjito, Nurkholis Hamidi, Uun Yanuhar, and I. N. G. Wardana. "Thermogravimetric kinetic analysis of Nannochloropsis oculata combustion in air atmosphere." Frontiers in Energy 9, no. 2 (February 6, 2015): 125–33. http://dx.doi.org/10.1007/s11708-015-0346-x.

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Pandeirada, Carolina O., Élia Maricato, Sónia S. Ferreira, Viviana G. Correia, Benedita A. Pinheiro, Dmitry V. Evtuguin, Angelina S. Palma, et al. "Structural analysis and potential immunostimulatory activity of Nannochloropsis oculata polysaccharides." Carbohydrate Polymers 222 (October 2019): 114962. http://dx.doi.org/10.1016/j.carbpol.2019.06.001.

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Zhu, Yan, and Nurhan Turgut Dunford. "Growth and Biomass Characteristics of Picochlorum oklahomensis and Nannochloropsis oculata." Journal of the American Oil Chemists' Society 90, no. 6 (March 16, 2013): 841–49. http://dx.doi.org/10.1007/s11746-013-2225-0.

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47

Genc Polat, Derya, Yasar Durmaz, Nevzat Konar, Omer Said Toker, Ibrahim Palabiyik, and Murat Tasan. "Using encapsulated Nannochloropsis oculata in white chocolate as coloring agent." Journal of Applied Phycology 32, no. 5 (July 24, 2020): 3077–88. http://dx.doi.org/10.1007/s10811-020-02205-1.

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48

Hong, Seong-Joo, Narae Yim, Mi-Ae Han, Danbee Yoo, and Choul-Gyun Lee. "Effect of Nitrogen Deficiency on Cell Growth and Fatty Acids Production of Nannochloropsis oculata K-1281." Journal of Marine Bioscience and Biotechnology 8, no. 2 (December 31, 2016): 45–53. http://dx.doi.org/10.15433/ksmb.2016.8.2.045.

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49

Salarzadeh, Alireza, and Elham Nahidi. "Evaluation of Growth and Survival of Artemia franciscana fed with Nannochloropsis oculata and Chlorella capsulata." International Journal of Life Sciences 10, no. 1 (February 10, 2016): 35–39. http://dx.doi.org/10.3126/ijls.v10i1.14507.

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Abstract:
Artemia franciscana was fed for seven days with 11.7, 23.4 and 46.8 mg l-1. d-1 (ash-free dry weight) of Nannochloropsis oculata and Chlorella capsulata, to evaluate the food value of these two microalgae. Survival was similar, close to or greater than 90% in all cases, but there were significant differences in growth beginning on the third day. The final ash-free dry weight of A. franciscana fed with N. oculata was seven times greater than with C. capsulata and the respective developmental stages were between instar 14 and 16 and from 8 to 9. Food ingestion was similar with the two diets, but the mean gross growth efficiencies were 80% with N. oculata and 7% with C. capsulata, indicating a poor assimilation of C. capsulata. Therefore, C. capsulata cannot be considered as a suitable diet for A. franciscana, because the amount of energy obtained is not sufficient to sustain normal growth.International Journal of Life Sciences 10 (1) : 2016; 35-39
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Kim, Junseong, H. H. Chaminda Lakmal, Ji-Hyeok Lee, WonWoo Lee, and You-Jin Jeon. "Anti-inflammatory and Anti-cancer Effects of Sterol-rich Fraction from Nannochloropsis oculata by using Saponification." Korean Journal of Fisheries and Aquatic Sciences 47, no. 6 (December 31, 2014): 770–75. http://dx.doi.org/10.5657/kfas.2014.0770.

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