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Journal articles on the topic 'Nanoparticle biogenesis'

Author: Grafiati

Published: 11 March 2023

Last updated: 31 July 2025

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1

Kousalya, Lavudi, Kokkanti Rekha Rani, Patnaik Srinivas, and Penchalaneni Josthna. "Biogenesis of Melia Azedarach silver nanoparticles using leaves and fruits in breast and ovarian cancer cell lines." Archive of Urological Research 7, no. 1 (2023): 008–16. http://dx.doi.org/10.17352/aur.000044.

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Folk medicine has been considered one of the novel remedies for treating cancers. Women's cancers are increasing worldwide, and disease recurrence has been a major threat all over the world. Our current study focused on the formation of silver nanoparticles (AgNPs) by organic methods and their chemo-preventive capacity against the breast (MCF-7) and ovarian cancer (PA-1) cell lines from humans by employing MTT, Flow cytometry, and migration assays. Plant extracts in organic nanoparticle production have become more common in recent years due to their benefits, including affordability, effective

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Hurwitz, Stephanie N., Meghan M. Conlon, Mark A. Rider, Naomi C. Brownstein, and David G. Meckes. "Nanoparticle analysis sheds budding insights into genetic drivers of extracellular vesicle biogenesis." Journal of Extracellular Vesicles 5, no. 1 (2016): 31295. http://dx.doi.org/10.3402/jev.v5.31295.

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Kodiha, Mohamed, Hicham Mahboubi, Dusica Maysinger, and Ursula Stochaj. "Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells." Nanobiomedicine 3 (January 1, 2016): 3. http://dx.doi.org/10.5772/62337.

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Cancer cells can take up gold nanoparticles of different morphologies. These particles interact with the plasma membrane and often travel to intracellular organelles. Among organelles, the nucleus is especially susceptible to the damage that is inflicted by gold nanoparticles. Located inside the nucleus, nucleoli are specialized compartments that transcribe ribosomal RNA genes, produce ribosomes and function as cellular stress sensors. Nucleoli are particularly prone to gold nanoparticle-induced injury. As such, small spherical gold nanoparticles and gold nanoflowers interfere with the transcr

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SHARMA, Bhawana, and Afroz ALAM. "Biogenesis of silver nanoparticles from the shoot extract of Delonix regia its characterization (UV–Vis spectroscopy and SEM) and evaluation for antimicrobial potential." Notulae Scientia Biologicae 15, no. 1 (2023): 11199. http://dx.doi.org/10.55779/nsb15111199.

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The current research work explores the production of silver nanoparticles using aqueous extracts of Delonix regia (Boj. ex Hook.) Raf. (Angiosperms; Fabaceae) shoots for the bioreduction of Ag metal and its antimicrobial activity. Fourier transform infrared spectroscopy (FTIR), Zeta potential, ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD) have been used to evaluate the produced silver nanoparticles (AgNPs). Both antibacterial and antifungal activity were examined against bacterial and fungal pathogens, viz., Escherichia coli and Bacillus subt

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Roychoudhury, Piya, Aleksandra Golubeva, Przemysław Dąbek, et al. "Diatom Mediated Production of Fluorescent Flower Shaped Silver-Silica Nanohybrid." Materials 14, no. 23 (2021): 7284. http://dx.doi.org/10.3390/ma14237284.

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Fabrication of flower-like nanostructures are gaining attention because of their high surface/volume ratio and extensive adsorption capacity. In the present investigation, flower-shaped, autofluorescent silver-silica (Ag-SiO2) hybrid nanoparticles have been fabricated exploiting diatoms as a source of nanosilica. Two different species of Gedaniella including G. flavovirens and G. mutabilis showed their efficacy in synthesizing fluorescent Ag-SiO2 nanoflowers (NFs) and nanospheres (NSs) against 9 mM silver nitrate solution, respectively. The biogenic nanoconjugate (Ag-SiO2) was characterized by

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Anghileri, G., W. DeVoogt, CS Seinen, et al. "DETERMINING THE FUNCTION OF MATRIX-BOUND AND SECRETED VESICLES IN MINERALIZATION." Orthopaedic Proceedings 106-B, SUPP_1 (2024): 144. http://dx.doi.org/10.1302/1358-992x.2024.1.144.

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Matrix-bound vesicles (MBVs) are embedded within osteoid and function as the site of initial mineral formation. However, they remain insufficiently characterised in terms of biogenesis, composition and function while their relationship with secreted culture medium EVs (sEVs) such as exosomes remains debated. We aimed to define the biogenesis and pro-mineralisation capacity of MBVs and sEVs to understand their potential in regenerative orthopaedics.sEVs and MBVs isolated from conditioned medium (differential ultracentrifugation) and ECM (collagenase digestion and differential ultracentrifugatio

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Amar kumar, Madhusudan Mahto, Shalu Priya, et al. "A comprehensive review of green synthesis, characterization and biomedical applications of silver nanoparticle synthesized using plant extracts." World Journal of Advanced Research and Reviews 22, no. 3 (2024): 1950–77. http://dx.doi.org/10.30574/wjarr.2024.22.3.1899.

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In recent years, the biogenesis of Nanoparticles has gained significant interest of the researchers. It can be attributed to the fact that Nanoparticles have varieties of application due to their unique physicochemical properties size surface morphology, charge, distribution pattern and capacity to act as a carrier of varieties of drug molecules.The present review provides a comprehensive survey of plant-mediated synthesis of AgNPs with specific focus on their applications, e.g., antimicrobial, anticancer, wound healing, drug delivery etc. as well as the characterization of nanoparticles using

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Amar, kumar, Mahto Madhusudan, Priya Shalu, et al. "A comprehensive review of green synthesis, characterization and biomedical applications of silver nanoparticle synthesized using plant extracts." World Journal of Advanced Research and Reviews 22, no. 3 (2024): 1950–77. https://doi.org/10.5281/zenodo.14765281.

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In recent years, the biogenesis of Nanoparticles has gained significant interest of the researchers. It can be attributed to the fact that Nanoparticles have varieties of application due to their unique physicochemical properties size surface morphology, charge, distribution pattern and capacity to act as a carrier of varieties of drug molecules.The present review provides a comprehensive survey of plant-mediated synthesis of AgNPs with specific focus on their applications, e.g., antimicrobial, anticancer, wound healing, drug delivery etc. as well as the characterization of nanoparticles using

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9

Murray, Matthew, Yazhe Wang, Ranjini K. Sundaram, Jason Beckta, W. Mark Saltzman, and Ranjit S. Bindra. "Abstract 294: Exploiting mutant PPM1D-induced metabolic defects with nanoparticle-encapsulated NAMPT inhibitors." Cancer Research 82, no. 12_Supplement (2022): 294. http://dx.doi.org/10.1158/1538-7445.am2022-294.

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Abstract Diffuse Intrinsic Pontine Glioma (DIPG) is a leading cause of death in pediatric cancer, with an abysmal &lt;1% 5-year survival rate due to lack of effective treatment options. A significant effort is needed to understand the genetic landscape of this disease to develop novel therapeutic strategies and modalities. Recently, the Bindra laboratory found that a truncation mutation in the gene PPM1D, found in ~30% of DIPG cases, causes global epigenetic alterations that lead to therapeutic vulnerabilities. They found that mutant PPM1D activity results in loss of the NAD+ biogenesis pr

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Selvarajan, Dr R., Ms Vaishnavi M, Ms Raksha B, Ms Deepikaa R, and Dr Durga M. "Green Synthesis of Nickel Oxide (Nio) Nanoparticles with Brassica oleracea Var. Capitata F. Rubra, Its Characterisation and Phytochemical Investigation." Saudi Journal of Medical and Pharmaceutical Sciences 11, no. 01 (2025): 58–64. https://doi.org/10.36348/sjmps.2025.v11i01.011.

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Most nanoparticles have diameters that lie within 1 to 100 nm. A variety of metal oxide nanoparticles can be synthesised chemically as well as biologically. NiO nanoparticles, which are synthesised chemically, tend to be highly toxic in their effects. In contrast, synthesis by the route of biogenesis or biomimetics is much more favourable than chemical synthesis. The pre-existing properties of purple cabbage are remarkably enhanced by NiO nanoparticles. Nickel oxide (NiO) nanoparticles were synthesised from the leaves of Brassica oleracea var. capitata. f. rubra (purple cabbage) by reducing Ni

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Arasi, Maria Beatrice, Francesca Pedini, Sonia Valentini, Nadia Felli, and Federica Felicetti. "Advances in Natural or Synthetic Nanoparticles for Metastatic Melanoma Therapy and Diagnosis." Cancers 12, no. 10 (2020): 2893. http://dx.doi.org/10.3390/cancers12102893.

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Advanced melanoma is still a major challenge in oncology. In the early stages, melanoma can be treated successfully with surgery and the survival rate is high, nevertheless the survival rate drops drastically after metastasis dissemination. The identification of parameters predictive of the prognosis to support clinical decisions and of new efficacious therapies are important to ensure patients the best possible prognosis. Recent progress in nanotechnology allowed the development of nanoparticles able to protect drugs from degradation and to deliver the drug to the tumor. Modification of the n

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Sancho-Albero, María, Maria del Mar Encabo-Berzosa, Manuel Beltrán-Visiedo, et al. "Efficient encapsulation of theranostic nanoparticles in cell-derived exosomes: leveraging the exosomal biogenesis pathway to obtain hollow gold nanoparticle-hybrids." Nanoscale 11, no. 40 (2019): 18825–36. http://dx.doi.org/10.1039/c9nr06183e.

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13

Babu, B. Hari, and G. Vijaya Lakshmi. "Antibacterial, Anticancer, Catalytic and Antioxidant Activities of Green Synthesized Silver Nanoparticles Derived from Alternanthera sessilis Leaf Extract." Asian Journal of Chemistry 34, no. 12 (2022): 3286–92. http://dx.doi.org/10.14233/ajchem.2022.23980.

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The current study evaluates the biogenesis of silver nanoparticles (Ag NPs) utilizing an aqueous extract of Alternanthera sessilis (Linn.) leaf. Biological nanoparticle production has recently gained appeal due to its eco-friendliness, simplicity, cost-effectiveness, non-hazardous nature and difficult circumstances. Aqueous extract of Alternanthera sessilis leaf contains terpenoids, carbohydrates and flavonoids to convert metal ions into metal and so stabilize the resultant nanoparticles. The UV-visible spectrophotometer obtained a distinctive peak at 420 nm, the XRD validated the crystalline

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Shaw, S., P. Singh, R. Mishra, R. Singh, R. Nayak, and S. Bose. "Cancer therapeutics strategy using nano-carrier mediated natural drugs." Journal of Achievements in Materials and Manufacturing Engineering 114, no. 1 (2022): 32–41. http://dx.doi.org/10.5604/01.3001.0016.1481.

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Nucleolin is a multifactorial protein, having a significant role in chromatin remodelling, mRNA stability, ribosome biogenesis, stemness, angiogenesis, etc., thus, it is potential therapeutic target in cancer. The purpose of this paper is to study porous silicon (pSi) nanocarrier-based natural drug delivery system targeting dysregulated nucleolin expression for cancer therapeutics. Quercetin was loaded in pre-synthesized and characterized pSi nanoparticles, and release kinetics was studied. The study compared the inhibitory concentration (IC50) of quercetin, synthetic drug doxorubicin, and que

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15

Liu, Shan, Angelica Ortiz, Juan Iovanna, and Max Costa. "Abstract 324: Nickel exposure alters extracellular vesicles biogenesis to carry NUPR1 and mediate cancer progression." Cancer Research 83, no. 7_Supplement (2023): 324. http://dx.doi.org/10.1158/1538-7445.am2023-324.

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Abstract Nickel (Ni), a group I human carcinogen by the International Agency for Research on Cancer (IARC) alters expression of various oncogenic molecules in cells resulting in adoption of cancer cell phenotypes, such as anchorage-independent growth, migration, and invasion. Cancer cell phenotypes are altered by internal genetic or epigenetic changes or in response to external signals mediated by soluble proteins or extracellular vesicles. However, few studies have linked extracellular vesicles (EVs) in response to environmental pollutants, such as Ni. Nuclear Protein 1 (NUPR1) is a stress re

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Kumar, Sanjay, Brennetta J. Crenshaw, Sparkle D. Williams, Courtnee’ R. Bell, Qiana L. Matthews, and Brian Sims. "Cocaine‐Specific Effects on Exosome Biogenesis in Microglial Cells." Neurochemical Research 46, no. 4 (2021): 1006–18. http://dx.doi.org/10.1007/s11064-021-03231-2.

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AbstractCocaine is a highly addictive stimulant and a well-known drug, with multiple effects on physiology. Cocaine can have direct effects on all cell types in the brain, including microglia. Microglia can be activated by other conditions, such as infection, inflammation, or injury. However, how cocaine regulates microglia and the influence of cocaine on microglial-derived exosomes remains unknown. Exosomes are nanovesicles that are responsible for intercellular communications, signaling, and trafficking necessary cargo for cell homeostasis. In this study, we hypothesized that cocaine affects

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Khaled, Annette R., Carolyn Dang, Lam Truong, et al. "Abstract 6584: Slamming the breaks on extracellular vesicles that cause breast cancer." Cancer Research 85, no. 8_Supplement_1 (2025): 6584. https://doi.org/10.1158/1538-7445.am2025-6584.

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Abstract Breast cancer (BC) recurrence and metastasis are significant causes of death, mainly due to the emergence of therapy-resistant cells. Secreted factors known as extracellular vesicles (EVs), especially small EVs (SEVs) or exosomes less than 100 nanometers in size, have been implicated in the plasticity associated with drug resistance, BC recurrence, and metastasis. EVs derived from BC cells can transform local or distal non-tumor cells, induce the epithelial-to-mesenchymal transition, and trigger uncontrolled proliferation, angiogenesis, and immune suppression of tumor cells. EVs also

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Huang, Di, Naagarajan Narayanan, Mario A. Cano-Vega, et al. "Nanoparticle-Mediated Inhibition of Notch Signaling Promotes Mitochondrial Biogenesis and Reduces Subcutaneous Adipose Tissue Expansion in Pigs." iScience 23, no. 6 (2020): 101167. http://dx.doi.org/10.1016/j.isci.2020.101167.

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Mustafa, Nurulhuda, Muhamad Irfan Azaman, and Wee-Joo Chng. "Daratumumab Resistant Natural Killer/T-Cell Lymphoma Exhibit an Addiction to the Exosome Biogenesis Pathway for Survival." Blood 138, Supplement 1 (2021): 2256. http://dx.doi.org/10.1182/blood-2021-151812.

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Abstract A phase 2 clinical trial has demonstrated that daratumumab monotherapy was safe and well tolerated in relapsed/refractory Natural Killer/T-Cell Lymphoma (NKTL). However, no patients achieved complete response, and duration of response was short. Similarly, in Multiple Myeloma (MM), while daratumumab based combinations are approved for front line treatment, responses are heterogeneous and development of treatment resistance inevitable. Therefore, elucidation of mechanisms which can overcome daratumumab resistance is essential for the optimization of therapeutic response in patients. To

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Ipinmoroti, Ayodeji O., Brennetta J. Crenshaw, Rachana Pandit, Sanjay Kumar, Brian Sims, and Qiana L. Matthews. "Human Adenovirus Serotype 3 Infection Modulates the Biogenesis and Composition of Lung Cell-Derived Extracellular Vesicles." Journal of Immunology Research 2021 (December 9, 2021): 1–19. http://dx.doi.org/10.1155/2021/2958394.

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Adenovirus (Ad) is a major causal agent of acute respiratory infections. However, they are a powerful delivery system for gene therapy and vaccines. Some Ad serotypes antagonize the immune system leading to meningitis, conjunctivitis, gastroenteritis, and/or acute hemorrhagic cystitis. Studies have shown that the release of small, membrane-derived extracellular vesicles (EVs) may offer a mechanism by which viruses can enter cells via receptor-independent entry and how they influence disease pathogenesis and/or host protection considering their existence in almost all bodily fluids. We proposed

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Lee, Young-Hee, Jeong-Seok Kim, Ji-Eun Kim та ін. "Nanoparticle mediated PPARγ gene delivery on dental implants improves osseointegration via mitochondrial biogenesis in diabetes mellitus rat model". Nanomedicine: Nanotechnology, Biology and Medicine 13, № 5 (2017): 1821–32. http://dx.doi.org/10.1016/j.nano.2017.02.020.

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22

Velázquez-Cervantes, Manuel Adrián, Arturo Flores-Pliego, Yazmín Rocío Benitez-Zeferino, et al. "Zika Virus Infection Modulates Extracellular Vesicle Biogenesis and Morphology in Human Umbilical Cord Endothelial Cells: A Proteomic and Microscopic Analysis." Microorganisms 13, no. 6 (2025): 1402. https://doi.org/10.3390/microorganisms13061402.

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Infection with Zika virus (ZIKV) is a perinatal health problem and a vertical infection that promotes neurological fetal damage. ZIKV infects different cellular components at the maternal–fetal interface, including umbilical cord endothelial cells (HUVECs). Extracellular vesicles (EVs) are cellular components that mediate extracellular communication. Viruses have the capacity to hijack and modify the biogenesis machinery of EVs for their own benefit. The present work provides proteomic results (2D electrophoresis) that show the regulation of the expression of proteins involved in autophagy, ox

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Wang, Yuting, Xian Shu, Jinyan Hou, et al. "Selenium Nanoparticle Synthesized by Proteus mirabilis YC801: An Efficacious Pathway for Selenite Biotransformation and Detoxification." International Journal of Molecular Sciences 19, no. 12 (2018): 3809. http://dx.doi.org/10.3390/ijms19123809.

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Selenite is extremely biotoxic, and as a result of this, exploitation of microorganisms able to reduce selenite to non-toxic elemental selenium (Se0) has attracted great interest. In this study, a bacterial strain exhibiting extreme tolerance to selenite (up to 100 mM) was isolated from the gut of adult Monochamus alternatus and identified as Proteus mirabilis YC801. This strain demonstrated efficient transformation of selenite into red selenium nanoparticles (SeNPs) by reducing nearly 100% of 1.0 and 5.0 mM selenite within 42 and 48 h, respectively. Electron microscopy and energy dispersive X

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Kumar, Ashish, Pawan Kumar, Mitu Sharma, et al. "Role of extracellular vesicles secretion in paclitaxel resistance of prostate cancer cells." Cancer Drug Resistance 5, no. 3 (2022): 612–24. http://dx.doi.org/10.20517/cdr.2022.26.

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Aim: The development of chemotherapy resistance is the major obstacle in the treatment of advanced prostate cancer (PCa). Extracellular vesicles (EVs) secretion plays a significant role among different mechanisms contributing to chemoresistance. Hence, inhibition of EVs release may increase the efficacy of chemotherapeutic drugs against PCa. Methods: Paclitaxel (PTX) resistant PCa cells (PC3-R and DU145-R) were treated with GW4869, a known exosome biogenesis inhibitor. EVs were isolated from the conditioned media by ExoQuick-based precipitation method and characterized for concentration and si

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Sanchez, Julio, Timothy Pierpont, Dariana Argueta-Zamora, Kristin Wilson, Avery August, and Richard Cerione. "CSIG-16. PTEN LOSS IN GLIOMA CELL LINES FACILITATES IMMUNE EVASION BY UPREGULATING EXTRACELLULAR VESICLE BIOGENESIS AND PD-L1 CARGO." Neuro-Oncology 25, Supplement_5 (2023): v43. http://dx.doi.org/10.1093/neuonc/noad179.0172.

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Abstract Phosphatase and tensin homologue (PTEN) is deleted or mutated in over 50% of all glioblastoma (GBM) cases. Recently, PTEN loss has been implicated as a prognostic biomarker for patients likely to be non-responders treated with immune checkpoint inhibitor (ICI) therapy. Indeed, a growing body of evidence has highlighted the loss of PTEN with immuno-modulatory functions including the upregulation of the programmed death ligand-1 (PD-L1) and the development of an immunosuppressive tumor immune microenvironment (TIME), likely the result of an altered secretome. This raised the question of

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Gul, Rukhsana, Mushtaq A. Dar, Shahid Nawaz, and Assim A. Alfadda. "Protective Effects of Nanoceria against Mitochondrial Dysfunction and Angiotensin II-Induced Hypertrophy in H9c2 Cardiomyoblasts." Antioxidants 12, no. 4 (2023): 877. http://dx.doi.org/10.3390/antiox12040877.

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Mitochondrial dysfunction triggered by increased reactive oxygen species (ROS) generation is involved in the pathogenesis and development of cardiac hypertrophy. Nanoceria (cerium oxide nanoparticle) has powerful ROS-scavenging properties and is considered a potential therapeutic option for curbing ROS-related disorders. Here, we explored the signaling mechanism underlying the protective effects of nanoceria against angiotensin (Ang) II-stimulated pathological response in H9c2 cardiomyoblasts. Our data revealed that pretreatment of H9c2 cardiomyoblasts with nanoceria significantly prevented An

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Verta, Roberta, Cristina Grange, Renata Skovronova, et al. "Generation of Spike-Extracellular Vesicles (S-EVs) as a Tool to Mimic SARS-CoV-2 Interaction with Host Cells." Cells 11, no. 1 (2022): 146. http://dx.doi.org/10.3390/cells11010146.

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Extracellular vesicles (EVs) and viruses share common features: size, structure, biogenesis and uptake. In order to generate EVs expressing the SARS-CoV-2 spike protein on their surface (S-EVs), we collected EVs from SARS-CoV-2 spike expressing human embryonic kidney (HEK-293T) cells by stable transfection with a vector coding for the S1 and S2 subunits. S-EVs were characterized using nanoparticle tracking analysis, ExoView and super-resolution microscopy. We obtained a population of EVs of 50 to 200 nm in size. Spike expressing EVs represented around 40% of the total EV population and co-expr

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Skryabin, G. O., A. V. Komelkov, P. B. Kopnin, I. I. Nikishin, S. A. Kuzmichev, and E. M. Tchevkina. "Effect of caveolin-1 knockdown on the protein composition of extracellular vesicles secreted by non-small cell lung cancer cells." Advances in Molecular Oncology 8, no. 1 (2021): 41–46. http://dx.doi.org/10.17650/2313-805x2021-8-1-41-46.

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Background. Recent data show evidence that lipid rafts (LR) proteins could be involved in the formation of exosomes and the sorting of proteins that make up the exosomal cargo. Such data are available for flotillins, structural and functional components of flatted rafts. The presence of the main component of caveolar rafts, caveolin-1 (Cav-1), has been shown in exosomes produced by some cancer cells; however, its possible participation in the regulation of the protein composition of exosomes has not been studied previously.Materials and methods. Knockdown of Cav-1 by transduction of a lentivir

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Gurunathan, Sangiliyandi, Min-Hee Kang, Muniyandi Jeyaraj, and Jin-Hoi Kim. "Palladium Nanoparticle-Induced Oxidative Stress, Endoplasmic Reticulum Stress, Apoptosis, and Immunomodulation Enhance the Biogenesis and Release of Exosome in Human Leukemia Monocytic Cells (THP-1)." International Journal of Nanomedicine Volume 16 (April 2021): 2849–77. http://dx.doi.org/10.2147/ijn.s305269.

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Gurunathan, Sangiliyandi, Muniyandi Jeyaraj, Min-Hee Kang, and Jin-Hoi Kim. "Melatonin Enhances Palladium-Nanoparticle-Induced Cytotoxicity and Apoptosis in Human Lung Epithelial Adenocarcinoma Cells A549 and H1229." Antioxidants 9, no. 4 (2020): 357. http://dx.doi.org/10.3390/antiox9040357.

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Palladium nanoparticles (PdNPs) are increasingly being used in medical and biological applications due to their unique physical and chemical properties. Recent evidence suggests that these nanoparticles can act as both a pro-oxidant and as an antioxidant. Melatonin (MLT), which also shows pro- and antioxidant properties, can enhance the efficacy of chemotherapeutic agents when combined with anticancer drugs. Nevertheless, studies regarding the molecular mechanisms underlying the anticancer effects of PdNPs and MLT in cancer cells are still lacking. Therefore, we aimed to investigate the potent

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Valentino, Taylor R., Blake D. Rule, C. Brooks Mobley, Mariana Nikolova-Karakashian, and Ivan J. Vechetti. "Skeletal Muscle Cell Growth Alters the Lipid Composition of Extracellular Vesicles." Membranes 11, no. 8 (2021): 619. http://dx.doi.org/10.3390/membranes11080619.

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We sought to characterize the lipid profile of skeletal muscle cell-derived Extracellular Vesicles (EVs) to determine if a hypertrophic stimulus would affect the lipid composition of C2C12 myotube-derived EVs. Analyses included C2C12 murine myoblasts differentiated into myotubes and treated with Insulin-Like Growth Factor 1 (IGF-1) for 24 h to induce hypertrophic growth. EVs were isolated from cell culture media, quantified using Nanoparticle Tracking Analysis (NTA) and analyzed using Transmission Electron Microscopy (TEM). EVs were homogenized and lipids extracted for quantification by Mass S

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Shekhawat, Mahipal S., M. Manokari, N. Kannan, J. Revathi, and R. Latha. "Synthesis of silver nanoparticles using Cardiospermum halicacabum L. leaf extract and their characterization." Journal of Phytopharmacology 2, no. 5 (2013): 15–20. http://dx.doi.org/10.31254/phyto.2013.2503.

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Present study reports the biogenesis of silver nanoparticles from the leaves of Cardiospermum halicacabum L. and their characterization. C. halicacabum has been used in traditional medicines but so far it has not been tested for synthesis of silver nanoparticles (AgNPs). The aqueous silver ions exposed to the herbal extract, which were reduced and the nanoparticles were synthesized. The presence of nanoparticles was confirmed by the formation of brown color of the reaction mixture. The brown color was observed after 25 minutes. The silver nanoparticules qualitatively characterized by UV-Visibl

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Warnier, Geoffrey, Estelle De Groote, Florian A. Britto, et al. "Effects of an acute exercise bout in hypoxia on extracellular vesicle release in healthy and prediabetic subjects." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 322, no. 2 (2022): R112—R122. http://dx.doi.org/10.1152/ajpregu.00220.2021.

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The purpose of this study is to investigate exosome-like vesicle (ELV) plasma concentrations and markers of multivesicular body (MVB) biogenesis in skeletal muscle in response to acute exercise. Seventeen healthy [body mass index (BMI): 23.5 ± 0.5 kg·m−2] and 15 prediabetic (BMI: 27.3 ± 1.2 kg·m−2) men were randomly assigned to two groups performing an acute cycling bout in normoxia or hypoxia ([Formula: see text] 14.0%). Venous blood samples were taken before (T0), during (T30), and after (T60) exercise, and biopsies from m. vastus lateralis were collected before and after exercise. Plasma EL

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Wang, Yanhong, Ying Song, Lijie Zhou, et al. "The Overexpression of TOB1 Induces Autophagy in Gastric Cancer Cells by Secreting Exosomes." Disease Markers 2022 (April 12, 2022): 1–10. http://dx.doi.org/10.1155/2022/7925097.

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We previously confirmed that transducer of ERBB2, 1 (TOB1) gene, can induce autophagy in gastric cancer cells. Studies have shown the biogenesis of exosomes overlaps with different autophagy processes, which helps to maintain the self-renewal and homeostasis of body cells. This study is aimed at verifying whether overexpressing TOB1 induces autophagy by secreting exosomes in gastric cancer cells and its underlying mechanisms. Differential ultracentrifugation was used to extracted the exosomes from the culture medium of gastric cancer cell line AGS-TOB1 ectopically overexpressing TOB1 (exo-AGS-

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Nguyen, Nghi M., Daniel Meyer, Luke Meyer, et al. "Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development." Cells 12, no. 6 (2023): 966. http://dx.doi.org/10.3390/cells12060966.

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Recently, the long-term use of sedative agents in the neonatal intensive care unit (NICU) has raised concerns about neurodevelopmental outcomes in exposed neonates. Midazolam (MDZ), a common neonatal sedative in the NICU, has been suggested to increase learning disturbances and cognitive impairment in children. However, molecular mechanisms contributing to such outcomes with long-term MDZ use during the early stages of life remain unclear. In this study, we for the first time elucidate the role of brain-derived extracellular vesicles (BDEVs), including mining the BDEV proteome post long-term M

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Pooja, Bansal, Singh Duhan Joginder, and Kumar Gahlawat Suresh. "Biogenesis of nanoparticles: A review." African Journal of Biotechnology 13, no. 28 (2014): 2778–85. http://dx.doi.org/10.5897/ajb2013.13458.

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Singh, Pushpendra Pratap, Nitai Debnath, Tripti Bhatnagar, Sumistha Das, and Sarika Chaturvedi. "Comparative Evaluation of Biogenesis of ZnO Nanoparticles Using Leaf Extracts of Bacopa monnieri, Acacia arabica and Catharanthus roseus." Indian Journal Of Science And Technology 16, no. 37 (2023): 3034–42. http://dx.doi.org/10.17485/ijst/v16i37.2050.

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Trivedi, Rucha, and Jamboor K. Vishwanatha. "Abstract 1314: Molecular contribution of Annexin A2 in triple negative breast cancer metastasis via tumor-derived extracellular vesicles." Cancer Research 83, no. 7_Supplement (2023): 1314. http://dx.doi.org/10.1158/1538-7445.am2023-1314.

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Abstract Triple negative breast cancer (TNBC) is the most lethal subtype of all breast cancers leading to high mortality rates. Tumor-derived extracellular vesicles (TEV) along with their cell-type specific cargo are greatly implicated in tissue-specific metastasis. In addition to metastasis, TEV play a crucial role in interacting with the distant microenvironment & shaping a pre-metastatic niche (PMN) for tumor cells to home. Understanding the mechanisms by which TEV promote PMN development has great potential to improve the diagnosis, prognosis, & treatment of TNBC. Annexin A2 (AnxA2

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Singh, Kiran, Olusola B. Sokefun, and Shweta Yadav. "A Compendious Prospective about Biogenesis of Nanoparticles and their Persuasions." Asian Journal of Chemistry 34, no. 4 (2022): 793–806. http://dx.doi.org/10.14233/ajchem.2022.23523.

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Nanotechnology cogitates manufacturing and exploitation of materials, in which all components are present at the nanoscale and their size ranges up to 100 nm. Nanoparticles can be synthesized chemically or biologically; nevertheless, biologically synthesized nanoparticles are less toxic to the environment and human health. Biogenesis of nanoparticles is acquiring momentum due to the use of biocompatible precursors’ viz., fungi, algae and various plant extracts. Nanoparticles are being useful in various branches of industrial products including energy storage and daily applications namely cosme

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Hoagstrom, Kristine V., Ying Huang, Gloria E. Borgstahl, and Michael A. Hollingsworth. "Abstract A075: Establishing the interaction mechanism of oncoprotein, Mucin1, and extracellular vesicle marker protein, Alix, in pancreatic cancer." Cancer Research 84, no. 17_Supplement_2 (2024): A075. http://dx.doi.org/10.1158/1538-7445.pancreatic24-a075.

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Abstract Pancreatic ductal adenocarcinoma (PDAC) remains an aggressive disease with devastating mortality rates largely due to a poor understanding of its progression mechanism. Small extracellular vesicles (sEVs) are used by cancer cells to aid in their progression, and tumor-derived EVs (TEVs) show a distinct protein cargo composition potentially contributing to this rapid progression and metastasis of PDAC. To investigate this phenomenon, we isolated sEVs from conditioned media of pancreatic cancer cell lines using ultracentrifugation and characterized them by transmission electron microsco

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Batool, Madiha, Walid M. Daoush, and Muhammad Khalid Hussain. "Dye Sequestration Using Biosynthesized Silver Nanoparticles Adsorbent in Aqueous Solutions." Crystals 12, no. 5 (2022): 662. http://dx.doi.org/10.3390/cryst12050662.

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Nanomaterials have gained much attention in the field of environmental remediation, largely due to their high surface area-to-volume ratio and other unique physical, chemical, and biological characteristics that emerge due to its size effects. Metallic nanoparticles are traditionally manufactured using wet chemical processes; however, the chemicals utilized are generally hazardous and combustible. The biosynthesis of nanoparticles using a variety of plant resources is considered a green technology because it does not use toxic chemicals. This work focuses on the green synthesis of biogenetic s

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Priya F., Janeeta, Leema Rose A., Vidhya S., Arputharaj A., Manimegalai V., and Durgadevi S. "Biogenesis of Metal Nanoparticles and its Potential Targeted Drug Delivery Systems for Urolithiasis." International Journal of Zoological Investigations 08, Spl 1 (2022): 23–28. http://dx.doi.org/10.33745/ijzi.2022.v08i0s1.003.

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Urinary stones are a biggest issue that people experience worldwide. Urinary tract infections that lead to struvite urinary stones are the result of improper organ function, particularly kidney. The present study concentrated on an economical and environmentally friendly method for producing copper nanoparticles utilizing Eclipta prostrata leaves extract as a reducing and capping agent. UV-Visible spectroscopy shows SPR band at 256 nm which was used to validate the synthesis of copper nanoparticles. According to FT-IR Spectroscopic investigations, phytoconstituents have a major role in the red

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Radtsig, M. A., O. A. Koksharova, V. A. Nadtochenko, and I. A. Khmel’. "Production of gold nanoparticles by biogenesis using bacteria." Microbiology 85, no. 1 (2016): 63–70. http://dx.doi.org/10.1134/s0026261716010094.

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Suganya, P., T. Pavithra, and G. Singaravelu. "Biogenesis of Hematite Nanoparticles Employing Prosopis cineraria and their Antioxidant Property." Asian Journal of Chemistry 34, no. 9 (2022): 2269–73. http://dx.doi.org/10.14233/ajchem.2022.23780.

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Interaction of green principles and metallic ions is a fascinating field of research to accomplish synthesis of biocompatible nanoparticles. In present study, Fe2O3 nanoparticles were fabricated utilizing phytochemical of wonder tree, Prosopis cineraria a natural herbal infusion. The leaf constituents of P. cineraria acted as reductant and stabilizer. The UV-vis spectrum showed characteristic SPR at 270 nm depicts the formation of iron oxide nanoparticles. Fourier transform infrared (FTIR) spectroscopy suggested that primary carboxylic acid, esters have involved in the synthesis. Further high-

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Hosseini, Mohammad Raouf, Mahin Schaffie, Mohammad Pazouki, Majid Lotfalian, Axel Schippers, and Mohammad Ranjbar. "Biogenesis of Nanoparticles with Potential Applications as Semiconductor from Chalcopyrite Concentrate." Advanced Materials Research 825 (October 2013): 92–95. http://dx.doi.org/10.4028/www.scientific.net/amr.825.92.

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Nanostructure forms of semiconductor materials are of great interest. Among these compounds, copper sulfide as a variable stoichiometric composition attracts considerable attention. In the present study, copper sulfide nanoparticles were synthesized biologically from a chalcopyrite concentratemainly containing chalcopyrite (46%) and pyrite (23%). Firstly, the copper contents of the concentrate were bioleached using thermophile bacteria, then the grownFusarium oxysporumwas added to the prepared solution and the biosynthesized nanoparticles collected and their characteristics compared with the p

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Anand, Shashi, Kunwar Somesh Vikramdeo, Mohammad Aslam Khan, Seema Singh, Moh'd Khushman, and Ajay Pratap Singh. "Abstract 1189: MYB-driven extracellular vesicle-associated microRNAs in pancreatic cancer: potential significance in pathobiology and biomarker development." Cancer Research 85, no. 8_Supplement_1 (2025): 1189. https://doi.org/10.1158/1538-7445.am2025-1189.

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Abstract The oncogenic transcription factor MYB is aberrantly expressed in pancreatic cancer (PC), where it plays a key role in tumor progression and metastasis. Our previous studies have demonstrated that MYB supports hypoxic survival through metabolic reprogramming and modulates the cellular secretome, highlighting its potential role in shaping the pancreatic tumor microenvironment (TME). Extracellular vesicles (EV), secreted by nearly all cell types, carry a wide range of biological molecules and serve as crucial mediators of intercellular communication. microRNAs (miRNAs) are among the mos

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Hublikar, Leena V., Sharanabasava V. Ganachari, Narasimha Raghavendra, et al. "Biogenesis of Silver Nanoparticles and Its Multifunctional Anti-Corrosion and Anticancer Studies." Coatings 11, no. 10 (2021): 1215. http://dx.doi.org/10.3390/coatings11101215.

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In the present research, silver nanoparticles were synthesized using ground nutshell and characterized using UV-visible, FTIR and PXRD. The SEM and HR-TEM aided in confirming the nano size, surface morphology and phase purity of the AgNPs. The quantum chemical, electrochemical, and structural studies were carried out to understand electrochemical properties. In addition, biological study such as anti-cancerous activity was carried out, and IC-50 values 80.25 µg/mL for A549 lung cancer cell lines. The effective electrochemical anti-corrosion activities were also studied. The majority constituen

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Anu, Kasi, Sandhanasamy Devanesan, Ramesh Prasanth, Mohamad S. AlSalhi, Singaravelu Ajithkumar, and Ganesan Singaravelu. "Biogenesis of selenium nanoparticles and their anti-leukemia activity." Journal of King Saud University - Science 32, no. 4 (2020): 2520–26. http://dx.doi.org/10.1016/j.jksus.2020.04.018.

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Singh, Vivek, Raina Singhmar, Meenu Rathi, Divya Bhatia, and Deepak Kumar Malik. "Biogenesis of Bacterial and Fungal Endophytic Mediated Silver Nanoparticles." Asian Journal of Biological and Life sciences 12, no. 1 (2023): 130–37. http://dx.doi.org/10.5530/ajbls.2023.12.18.

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Qi, Yi, Ru Ma, Xueyan Li, et al. "Disturbed mitochondrial quality control involved in hepatocytotoxicity induced by silica nanoparticles." Nanoscale 12, no. 24 (2020): 13034–45. http://dx.doi.org/10.1039/d0nr01893g.

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SiNPs triggered hepatocytotoxicity through interfering mitochondrial quality control process, including imbalanced mitochondrial dynamics, disturbed mitophagy and suppressed biogenesis, leading to mitochondrial dysfunction and ensuing cell damage.

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