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Academic literature on the topic 'Nanophotometer'
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Journal articles on the topic "Nanophotometer"
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Nisha, Rana, and Jain Seema. "Analysis of DNA Content of Wild and Cultured Labeo Calbasu Hamilton, 1822 Using Nanophotometer." International Journal of Trend in Scientific Research and Development 3, no. 1 (2018): 906–9. https://doi.org/10.31142/ijtsrd19105.
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Labeo calbasu is an important food fish and commonly known as the "calbasu" or "Black Rohu". The present study was conducted on DNA extraction and determination of DNA quantity of both male and female of wild and cultured Labeo calbasu using Nanophotometer. DNA isolation was done and gel electrophoresis was carried out. Extracted DNA was analyzed using nanophotometer Nanophotometer P330 Implen, Germany to determine the concentration of DNA and its purity level. Several different methods have been employed in the quantification of nuclear DNA over the past 50 years. The ease of use of this technique not only makes it a feasible option for small volume analysis of DNA but also a practical alternative for spectrophotometric measurement. Significant progress was made to measure micro volume liquid samples 1µl in biotechnology and pharmaceutical applications. For the present study both male and female of wild Labeo calbasu from middle Ganga Region and cultured Labeo calbasu from culture ponds and hatchery were taken. The value of DNA concentration in female of wild Labeo calbasu was in between 58 62 ng µl and of male was in between range of 64 68 ng µl. The value of DNA concentration in female of cultured Labeo calbasu was 60 66 ng µl and of male 68 74 ng µl. Nisha Rana | Seema Jain "Analysis of DNA Content of Wild and Cultured Labeo Calbasu (Hamilton, 1822) Using Nanophotometer" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-1 , December 2018, URL: https://www.ijtsrd.com/papers/ijtsrd19105.pdf
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Sophian, Alfi, and Andi Syukur. "Analysis of Purity and Concentration of Isolated DNA in Making Raw DNA of Rat Species." Eruditio : Indonesia Journal of Food and Drug Safety 1, no. 2 (2021): 1–5. http://dx.doi.org/10.54384/eruditio.v1i2.75.
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Analysis of the purity and concentration of isolated DNA in the manufacture of standard rat DNA was carried out to see whether the isolation carried out could produce good quality DNA. The purpose of this study is to provide information on the manufacture of raw DNA in species DNA testing where the raw material that has been purchased so far made from synthetic materials can be more economical if using DNA material derived from the meat raw material of the target species. The DNA extraction method used is the column spin method or column centrifuge using the Intron Patho Gene-Spin (Viral DNA/RNA) extraction kit. Analysis method of concentration and purity of isolated DNA was analyzed based on the average value of concentration and purity which was read using a nanophotometer. Based on the results of the research conducted, the results of the isolated DNA concentration values were in the concentration range of 41,250 ng/
 mL to 42,300 ng/mL, with the average concentration of isolated DNA was 41,777 ng/mL. For the value of the purity of the isolated DNA whose absorbance was read using a nanophotometer at a wavelength of A260/A280, the results were between 2,301 to 2,384 with the average value of purity being at 2,326. This study concludes that all the extracted samples that showed the results of the DNA analysis produced were included in the good DNA category.
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Rana, Nisha, and Seema Jain. "Analysis of DNA Content of Wild and Cultured Labeo Calbasu Hamilton, 1822 Using Nanophotometer." International Journal of Trend in Scientific Research and Development Volume-3, Issue-1 (2018): 906–9. http://dx.doi.org/10.31142/ijtsrd19105.
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Utaminingsih, Sri, Sofia Dyah Utami, and Alfi Sophian. "Isolasi DNA pada Produk Otak-Otak Ikan Bandeng." Muhammadiyah Journal of Nutrition and Food Science (MJNF) 3, no. 1 (2022): 36. http://dx.doi.org/10.24853/mjnf.3.1.36-41.
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Latar belakang: Analisis mutu DNA hasil isolasi merupakan teknik analisis yang digunakan untuk mengetahui kualitas DNA hasil isolasi sehingga meminimalkan kegagalan proses amplifikasi dalam analisis molekuler. Tujuan: Tujuan dilakukan penelitian ini adalah untuk melihat mutu DNA hasil isolasi pada produk pangan olahan ikan berupa otak-otak ikan bandeng. Prosedur ekstraksi DNA mengikuti protokol ekstraksi manual kit yang digunakan. Analisis mutu DNA hasil isolasi diukur menggunakan nanophotometer, dimana analisis mutu berdasarkan nilai konsentrasi dan kemurnian DNA hasil isolasi. Analisis data dilakukan menggunakan uji rata-rata nilai konsentrasi dan kemurnian. Hasil: Berdasarkan hasil analisis mutu DNA diperoleh hasil konsentrasi DNA berada pada kisaran 9.6 – 18.5 dengan nilai rata-rata DNA hasil isolasi berada pada nilai 13.4 ng/µL, sedangkan nilai kemurnian yang dibaca pada panjang gelombang A260/A28 berada pada kisaran 1.64 – 1.87, dengan nilai rata-rata berada pada nilai 1.79. Simpulan: Sehingga dapat disimpulkan bahwa DNA hasil isolasi yang dilakukan pada sampel produk pangan olahan otak-otak ikan bandeng menunjukkan nilai konsentrasi dan kemurnian yang masuk dalam kategori hasil isolasi DNA yang baik.
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Utami, Sofia Dyah, Sri Utaminingsih, and Alfi Sophian. "Analisis DNA Hasil Isolasi Pada Produk Pangan Olahan Ikan (Surimi Ikan) Menggunakan Nano Photometer." JRST (Jurnal Riset Sains dan Teknologi) 7, no. 1 (2023): 9. http://dx.doi.org/10.30595/jrst.v7i1.15180.
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Analisis mutu DNA untuk melihat kualitas DNA hasil isolasi merupakan sebuah teknik analisis yang dapat digunakan untuk mendukung pengujian bidang biologi molekuler yang menggunakan instrumen PCR atau real-time PCR. Tujuan dari penelitian ini adalah untuk menganalisis kualitas DNA hasil isolasi pada sampel produk pangan olahan ikan. Manfaat penelitian ini diharapkan dapat menjadi sumber informasi yang mendukung penelitian di bidang molekuler pada ruang lingkup pengawasan mutu dan autentifikasi pada produk pangan olahan ikan. Analisis DNA hasil isolasi dianalisis menggunakan NanoPhotometer untuk mengukur nilai konsentrasi dan kemurnian dengan panjang gelombang A260/A280. Data yang diperoleh dari hasil pengukuran kemudian diinterpretasikan menggunakan nilai uji rata-rata untuk menganalisis mutu DNA hasil isolasi. Data hasil penelitian menunjukan nilai konsentrasi DNA berada pada kisaran 14,5 – 17,8 dengan rata-rata nilai konsentrasi berada pada 16,1. Untuk nilai kemurnian DNA hasil isolasi berada pada kisaran 1,89 – 2.07 dengan rata-rata nilai kemurnian berada pada 1,97. Dengan hasil tersebut maka dapat disimpulkan bahwa hasil isolasi DNA yang diukur menggunakan nano fotometer menunjukkan nilai kualitas DNA hasil isolasi yang baik.
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Sophian, Alfi, Sri Utaminingsih, and Sofia Dyah Utami. "Analysis of the Quality of Isolated DNA in the Making of Guinea Pig DNA Test Standards." Sainstek : Jurnal Sains dan Teknologi 15, no. 2 (2023): 74. http://dx.doi.org/10.31958/js.v15i2.7732.
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Analysis of the quality of DNA isolated from the manufacture of species DNA standards is one way to determine the quality of independently made species-specific DNA used in testing species DNA detection. This research was conducted to assess the quality of DNA isolated from the production of guinea pig DNA test standards. The benefit of this research is to provide an alternative reference for manufacturing species DNA standards. Moreover, this research is expected to enrich the methods of testing the quality of DNA isolated from different existing methods, using various test matrices. The DNA isolation method was carried out using the Dneasy Mericon Food kit. The quality of the isolated DNA was analyzed using a nanophotometer, measuring the parameters of concentration and purity values at the A260/A280 ratio. The results of DNA isolation indicated a concentration value of the isolated DNA ranging from 230.2 ng/µL to 238.5 ng/µL, with an average concentration value of 233.7 ng/µL. The purity value, read at the A260/A280 ratio, falls within the range of 2.04 – 2.11. Based on the study's results, it was found that the isolated DNA exhibited good DNA quality, making the DNA test standard suitable for use as a benchmark in species DNA testing.
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Emilia, Emilia, and Ashabul Anhar. "Optimalisasi Metode Ekstraksi DNA Daun, Kulit Kayu dan Kayu Pinus merkusii." Jurnal Ilmiah Mahasiswa Pertanian 6, no. 4 (2021): 766–78. http://dx.doi.org/10.17969/jimfp.v6i4.18233.
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Abstrak. Keberhasilan ekstraksi DNA sangatlah penting dalam proses investigasi genetika molekuler. Penggunaan sampel biasanya berupa organ tanaman muda karena diketahui dapat menghasilkan ekstrak DNA yang bagus. Kayu dan kulit kayu juga berpotensi sebagai sumber DNA karena sifatnya yang mudah didapat. Namun pengujian efektifitasnya untuk spesies Pinus merkusii belum pernah dilaporkan. Tujuan penelitian untuk membandingkan hasil ekstraksi DNA dari tiga jenis sampel dari spesies Pinus merkusii menggunakan tiga metode ekstraksi yaitu metode CTAB, CTAB dengan mercaptoethanol dan DNeasy Plant Mini Kit. Hasil ekstraksi dikuantifikasi menggunakan alat nanophotometer dan divisualisasi dalam gel agarosa. Dari ketiga metode ekstraksi DNA, metode CTAB memperoleh hasil kemurnian DNA yang lebih bagus baik pada uji kuantitas maupun uji kualitas. Semua metode memperoleh nilai optical density ratios di luar rentang kemurnian (λ260/280 = 1,8 – 2,0 ng/µl dan λ260/230 = 2,0 ng/µl) pada sampel kayu, kulit kayu dan sampel daun khusus metode DNeasy Plant Mini Kit dan memperoleh nilai optical density ratios mencapai 1,8-2,0 ng/µl pada sampel daun dari metode CTAB dan metode CTAB dengan mercaptoethanol. Hasil ekstraksi DNA pada metode CTAB dan metode CTAB dengan menggunakan mercaptoethanol tidak menunjukkan hasil pita DNA yang dapat diinterpretasikan sama sekali, sedangkan pada metode DNeasy Plant Mini Kit menunjukkan pita DNA pada sampel KK3 dan D3 dan pada sampel lainnya juga tidak menunjukkan pita DNA yang dapat diinterpretasikan.Kata Kunci: CTAB, Ekstraksi, DNA, DNeasy Plant Mini KitAbstract. The success of DNA extraction is very important in the process of genetic investigations molecular. The use of samples is usually in the form of young plant organs because it is known can produce a good DNA extract. Wood and bark are also potential as source of DNA because it is easy to obtain. However, testing its effectiveness for species of Pinus merkusii has never been reported. The aim of the study is to compare the results of DNA extraction from three types of samples from the Pinus merkusii species using three the extraction methods are the CTAB method, CTAB with mercaptoethanol and DNeasy Plant Mini Kits. The extraction result were quantified using a nanophotometer and visualized in agarose gel. Of the three DNA extraction methods, the CTAB. Method obtain better DNA purity results in both the quantity test and the test quality. All methods obtain optical density ratios outside the purity range (λ260/280 = 1.8-2.0 ng/µl and λ260/230 = 2.0 ng/µl) on wood, bark and leaf samples from specifically for the DNeasy Plant Mini Kit method and obtained optical dencity values ratios reached 1.8-2.0 ng/ µl in leaf samples from the CTAB method and the CTAB. Method with mercaptoethanol. The result of DNA extraction on the CTAB method and the CTAB. Method using mercaptoethanol did not show the result of DNA bands that cound interpreted at all, whereas in the DNeasy Plant Mini Kit method, showsan interpretable DNA band.Keywords: CTAB, Extraction, DNA, DNeasy Plant Mini Kit
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Sophian, Alfi, Sri Utaminingsih, Tirta Setya Bhakti, and Dewi Rahmawati. "Detection of porcine species DNA on meat processed food samples (shredded meat) using Real-Time PCR." Journal of halal product and research 5, no. 1 (2022): 32–37. http://dx.doi.org/10.20473/jhpr.vol.5-issue.1.32-37.
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DNA detection of porcine species in processed meat samples (shredded meat) was carried out as an effort to enrich the literature and reference alternative testing methods in the control of halal products circulating in Indonesia. The purpose of this study was to detect the DNA of porcine species in samples using real-time PCR. The research method used is a qualitative technique. This technique analyzes the CT value of the sample compared to the positive control by looking at the formation of the sigmoid curve if amplification occurs. The controls used consisted of negative control, positive control, other DNA control, and LOD control. The extraction technique used is the column centrifuge technique. The extraction results were then measured using a nano photometer to see the purity and concentration of the extracted DNA. Based on the research, it is known that the extraction results read on a nanophotometer at a wavelength of A260 / A280 show a concentration of 24,350 with a purity value of 21,164. For sample testing, the results of real-time PCR analysis showed the sample was detected at CT 39.77, positive control at CT 31.66, and LOD at 28.32. for negative control and specificity using other DNA was not detected. The conclusion of this research is that the sample used can be detected by the presence of porcine DNA.
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Ismail, Yulia Sari, Cut Yulvizar, Risa Riani Ramlan, and Mahyuddin Mahyuddin. "DNA isolation of potentially probiotic lactic acid bacteria from durian arilus fermentation (jruek drien)." Current Research on Biosciences and Biotechnology 1, no. 1 (2019): 34–38. http://dx.doi.org/10.5614/crbb.2019.1.1/uejb8271.
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Jruek drien is an Acehnese traditional fermented food containing lactic acid bacteria (LAB) from durian arilus fermentation. Lactic acid bacteria from jruek drien were considered as potential probiotics. The LAB from jruek drien were first identified by phenotyping and genotyping analysis. The aim of the research is to isolate DNA from lactic acid bacteria (JD-2 and JD-3) from jruek drien for identification based on 16S rDNA gene sequence analysis. The research steps consist of samples regeneration in MRSA medium with temperature of incubation at 37°C, samples culturing in TSB medium, DNA isolation, and measurement of the DNA concentration and DNA purity using nanophotometer. The result showed that the JD-2 and the JD-3 isolates grow well on selective media MRSA by incubation temperature at 37°C. JD-2 has a DNA concentration of 40.0 ng/µl with a purity level of 2.0, and JD-3 has a DNA concentration of 32.5 ng/µl with a purity level of 1.8 at the A260/A280 ratio. The 16S rRNA gene of JD-2 isolate was successfully amplified at 1426 bp and JD-3 isolate at 1435 bp. The JD-2 isolate was identified as Bacillus subtilis because it has the highest similarity with Bacillus subtilis strain WA3-4. The JD-3 isolate was identified as Lactobacillus plantarum because it has the highest similarity with L. plantarum strain CSI9 and strain CSI3.
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Sophian, Alfi, Utaminingsih Sri, and Utami Dyah Sofia. "DNA isolation in processed chicken meat products (nugget) using modified DNeasy Mericon Food kit (Qiagen)." HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY 12, no. 2 (2022): 15–21. http://dx.doi.org/10.46223/hcmcoujs.tech.en.12.2.2463.2022.
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DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets). The novelty of this research lies in the DNA isolation technique modified from the manual kit which is used to produce good-quality DNA isolation results. The extraction method used was the modified extraction method and the standard extraction method of the DNeasy Mericon Food kit. Analysis of DNA quality was measured using a nanophotometer, where the analysis of DNA quality was analyzed based on the parameters of concentration values and purity values. Based on research data, it is known that the modified DNA extraction method produces a concentration value of DNA isolation with an average concentration value of 1295.1 ng/µL. Meanwhile, the value of the purity of the isolated DNA was 2.10 on average. while the standard DNA extraction method resulted in an average DNA isolation concentration value of 111.53 ng/µL. While the value purity of the isolated DNA was at an average value of 1.926. From the research data analyzed, it was found that the modified DNA isolation method has the advantage that the concentration of isolated DNA obtained is higher than the standard method, while the purity value of the DNA isolated from the standard method has better purity when compared to the modified method.