Academic literature on the topic 'Nanozymes'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Nanozymes.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Nanozymes"
1
Liu, Jinjin, and Xiangheng Niu. "Rational Design of Nanozymes Enables Advanced Biochemical Sensing." Chemosensors 10, no. 10 (September 23, 2022): 386. http://dx.doi.org/10.3390/chemosensors10100386.
Full textAbstract:
In comparison with bioenzymes, nanozymes exhibit excellent robustness against extreme conditions, a low production cost, and easy-to-adjust properties, as well as potential versatility. These superiorities have attracted abundant interest in the last 15 years, to develop various nanozymes for applications including analytical sensing, environmental engineering, and biomedicine. In particular, for analytical sensing, a lot of nanozyme-involved principles and methods have been explored and applied to clinical diagnosis, environmental monitoring, food safety detection, and forensic analysis. Moreover, rational exploitation and use of nanozyme materials promote the performance of analytical methods. To highlight the latest progress in this attractive field, recent design concepts of nanozymes for advanced biochemical sensing are summarized. The development of single-atom nanozymes, self-cascade nanozymes, structurally biomimetic nanozymes, molecularly imprinted nanozymes, nanozymes breaking the pH limit, and multifunctional nanozymes is discussed in detail, to enhance detection sensitivity and selectivity, as well as expand application scenarios. Finally, some challenges and trends related to nanozyme-based sensors are reported, to satisfy the increasing needs of biochemical analysis with nanozymes.
2
Wang, Xin, Yuancong Xu, Nan Cheng, Xinxian Wang, Kunlun Huang, and Yunbo Luo. "Recent Advances in Nucleic Acid Modulation for Functional Nanozyme." Catalysts 11, no. 5 (May 17, 2021): 638. http://dx.doi.org/10.3390/catal11050638.
Full textAbstract:
Nanozymes have the potential to replace natural enzymes, so they are widely used in energy conversion technologies such as biosensors and signal transduction (converting biological signals of a target into optical, electrical, or metabolic signals). The participation of nucleic acids leads nanozymes to produce richer interface effects and gives energy conversion events more attractive characteristics, creating what are called “functional nanozymes”. Since different nanozymes have different internal structures and external morphological characteristics, functional modulation needs to be compatible with these properties, and attention needs to be paid to the influence of nucleic acids on nanozyme activity. In this review, “functional nanozymes” are divided into three categories, (nanozyme precursor ion)/ (nucleic acid) self-assembly, nanozyme-nucleic acid irreversible binding, and nanozyme-nucleic acid reversible binding, and the effects of nucleic acids on modulation principles are summarized. Then, the latest developments of nucleic acid-modulated nanozymes are reviewed in terms of their use in energy conversion technology, and their conversion mechanisms are critically discussed. Finally, we outline the advantages and limitations of “functional nanozymes” and discuss the future development prospects and challenges in this field.
3
Li, Dan, Ling Xia, and Gongke Li. "Recent Progress on the Applications of Nanozyme in Surface-Enhanced Raman Scattering." Chemosensors 10, no. 11 (November 7, 2022): 462. http://dx.doi.org/10.3390/chemosensors10110462.
Full textAbstract:
Nanozymes are nanomaterial with natural enzyme-like activity and can catalyze specific reactions for analyte identification and detection. Compared to natural enzymes, they have several benefits, including being steady, low-cost, easy to prepare and store. Based on the promising development of nanozymes in surface-enhanced Raman scattering (SERS), this paper reviews the classification of different types of nanozymes in SERS, including metal-based nanozyme, carbon-based nanozyme, metal-organic framework (MOF)/covalent organic framework (COF)-based nanozyme, and semiconductor-based nanozyme, followed by a detailed overview of their SERS applications in disease diagnosis, food safety, and environmental safety. Finally, this paper discusses the practical shortcomings of nanozymes in SERS applications and makes some suggestions for further research.
4
Luo, Yaying, Haiming Luo, Sijia Zou, Jing Jiang, Demin Duan, Lei Chen, and Lizeng Gao. "An In Situ Study on Nanozyme Performance to Optimize Nanozyme-Strip for Aβ Detection." Sensors 23, no. 7 (March 24, 2023): 3414. http://dx.doi.org/10.3390/s23073414.
Full textAbstract:
The nanozyme-strip is a novel POCT technology which is different from the conventional colloidal gold strip. It primarily utilizes the catalytic activity of nanozyme to achieve a high-sensitivity detection of target by amplifying the detection signal. However, previous research has chiefly focused on optimizing nanozyme-strip from the perspective of increasing nanozyme activity, little is known about other physicochemical factors. In this work, three sizes of Fe3O4 nanozyme and three sizes of CoFe2O4 nanozyme were used to investigate the key factors of nanozyme-strip for optimizing and improving its detection performance. We found that three sizes of Fe3O4 nanozyme all gather at the bottom of the nitrocellulose (NC) membrane, and three sizes of CoFe2O4 nanozyme migrate smoothly on the NC membrane, respectively. After color development, the surface of NC membranes distributed with CoFe2O4 peroxidase nanozymes had significant color change. Experimental results show that CoFe2O4 nanozymes had better dispersity than Fe3O4 nanozymes in an aqueous solution. We observed that CoFe2O4 nanozymes with smaller particle size migrated to the middle of the NC membrane with a higher number of particles. According to the results above, 55 ± 6 nm CoFe2O4 nanozyme was selected to prepare the nanozyme probe and achieved a highly sensitive detection of Aβ42Os on the nanozyme-strip. These results suggest that nanozyme should be comprehensively evaluated in its dispersity, the migration on NC membrane, and the peroxidase-like activity to determine whether it can be applied to nanozyme-strip.
5
Kurup, Chitra Padmakumari, and Minhaz Uddin Ahmed. "Nanozymes towards Personalized Diagnostics: A Recent Progress in Biosensing." Biosensors 13, no. 4 (April 5, 2023): 461. http://dx.doi.org/10.3390/bios13040461.
Full textAbstract:
This review highlights the recent advancements in the field of nanozymes and their applications in the development of point-of-care biosensors. The use of nanozymes as enzyme-mimicking components in biosensing systems has led to improved performance and miniaturization of these sensors. The unique properties of nanozymes, such as high stability, robustness, and surface tunability, make them an attractive alternative to traditional enzymes in biosensing applications. Researchers have explored a wide range of nanomaterials, including metals, metal oxides, and metal–organic frameworks, for the development of nanozyme-based biosensors. Different sensing strategies, such as colorimetric, fluorescent, electrochemical and SERS, have been implemented using nanozymes as signal-producing components. Despite the numerous advantages, there are also challenges associated with nanozyme-based biosensors, including stability and specificity, which need to be addressed for their wider applications. The future of nanozyme-based biosensors looks promising, with the potential to bring a paradigm shift in biomolecular sensing. The development of highly specific, multi-enzyme mimicking nanozymes could lead to the creation of highly sensitive and low-biofouling biosensors. Integration of nanozymes into point-of-care diagnostics promises to revolutionize healthcare by improving patient outcomes and reducing costs while enhancing the accuracy and sensitivity of diagnostic tools.
6
Zhu, Weisheng, Luyao Wang, Qisi Li, Lizhi Jiao, Xiaokan Yu, Xiangfan Gao, Hao Qiu, Zhijun Zhang, and Wei Bing. "Will the Bacteria Survive in the CeO2 Nanozyme-H2O2 System?" Molecules 26, no. 12 (June 19, 2021): 3747. http://dx.doi.org/10.3390/molecules26123747.
Full textAbstract:
As one of the nanostructures with enzyme-like activity, nanozymes have recently attracted extensive attention for their biomedical applications, especially for bacterial disinfection treatment. Nanozymes with high peroxidase activity are considered to be excellent candidates for building bacterial disinfection systems (nanozyme-H2O2), in which the nanozyme will promote the generation of ROS to kill bacteria based on the decomposition of H2O2. According to this criterion, a cerium oxide nanoparticle (Nanoceria, CeO2, a classical nanozyme with high peroxidase activity)-based nanozyme-H2O2 system would be very efficient for bacterial disinfection. However, CeO2 is a nanozyme with multiple enzyme-like activities. In addition to high peroxidase activity, CeO2 nanozymes also possess high superoxide dismutase activity and antioxidant activity, which can act as a ROS scavenger. Considering the fact that CeO2 nanozymes have both the activity to promote ROS production and the opposite activity for ROS scavenging, it is worth exploring which activity will play the dominating role in the CeO2-H2O2 system, as well as whether it will protect bacteria or produce an antibacterial effect. In this work, we focused on this discussion to unveil the role of CeO2 in the CeO2-H2O2 system, so that it can provide valuable knowledge for the design of a nanozyme-H2O2-based antibacterial system.
7
Stasyuk, Nataliya, Oleh Smutok, Olha Demkiv, Tetiana Prokopiv, Galina Gayda, Marina Nisnevitch, and Mykhailo Gonchar. "Synthesis, Catalytic Properties and Application in Biosensorics of Nanozymes and Electronanocatalysts: A Review." Sensors 20, no. 16 (August 12, 2020): 4509. http://dx.doi.org/10.3390/s20164509.
Full textAbstract:
The current review is devoted to nanozymes, i.e., nanostructured artificial enzymes which mimic the catalytic properties of natural enzymes. Use of the term “nanozyme” in the literature as indicating an enzyme is not always justified. For example, it is used inappropriately for nanomaterials bound with electrodes that possess catalytic activity only when applying an electric potential. If the enzyme-like activity of such a material is not proven in solution (without applying the potential), such a catalyst should be named an “electronanocatalyst”, not a nanozyme. This paper presents a review of the classification of the nanozymes, their advantages vs. natural enzymes, and potential practical applications. Special attention is paid to nanozyme synthesis methods (hydrothermal and solvothermal, chemical reduction, sol-gel method, co-precipitation, polymerization/polycondensation, electrochemical deposition). The catalytic performance of nanozymes is characterized, a critical point of view on catalytic parameters of nanozymes described in scientific papers is presented and typical mistakes are analyzed. The central part of the review relates to characterization of nanozymes which mimic natural enzymes with analytical importance (“nanoperoxidase”, “nanooxidases”, “nanolaccase”) and their use in the construction of electro-chemical (bio)sensors (“nanosensors”).
8
Wang, Lijun, Hong Zhou, Haixia Hu, Qin Wang, and Xianggui Chen. "Regulation Mechanism of ssDNA Aptamer in Nanozymes and Application of Nanozyme-Based Aptasensors in Food Safety." Foods 11, no. 4 (February 14, 2022): 544. http://dx.doi.org/10.3390/foods11040544.
Full textAbstract:
Food safety issues are a worldwide concern. Pathogens, toxins, pesticides, veterinary drugs, heavy metals, and illegal additives are frequently reported to contaminate food and pose a serious threat to human health. Conventional detection methods have difficulties fulfilling the requirements for food development in a modern society. Therefore, novel rapid detection methods are urgently needed for on-site and rapid screening of massive food samples. Due to the extraordinary properties of nanozymes and aptamers, biosensors composed of both of them provide considerable advantages in analytical performances, including sensitivity, specificity, repeatability, and accuracy. They are considered a promising complementary detection method on top of conventional ones for the rapid and accurate detection of food contaminants. In recent years, we have witnessed a flourishing of analytical strategies based on aptamers and nanozymes for the detection of food contaminants, especially novel detection models based on the regulation by single-stranded DNA (ssDNA) of nanozyme activity. However, the applications of nanozyme-based aptasensors in food safety are seldom reviewed. Thus, this paper aims to provide a comprehensive review on nanozyme-based aptasensors in food safety, which are arranged according to the different interaction modes of ssDNA and nanozymes: aptasensors based on nanozyme activity either inhibited or enhanced by ssDNA, nanozymes as signal tags, and other methods. Before introducing the nanozyme-based aptasensors, the regulation by ssDNA of nanozyme activity via diverse factors is discussed systematically for precisely tailoring nanozyme activity in biosensors. Furthermore, current challenges are emphasized, and future perspectives are discussed.
9
WANG, Erkang. "(Keynote, Digital Presentation) A Study of Nanozyme-Based Biosensor." ECS Meeting Abstracts MA2022-01, no. 53 (July 7, 2022): 2193. http://dx.doi.org/10.1149/ma2022-01532193mtgabs.
Full textAbstract:
Nanozymes have attracted significant research interest over the world due to their simple preparation, storage, as well as the low-cost compared with natural enzymes. We started to study nanozymes over 10 years ago [1-8]. A few examples of novel nanozymes from our laboratory are shown: 1, NiPd hNPs exhibited triple-enzyme mimetic activities (oxidase-like, peroxidase-like and catalase-like activities). 2, Fe3O4 NPs on 3D porous graphene exhibited enhanced nanozyme activity and used for glucose determination. 3, GOx@ZIF-8(NiPd) nanoflower exhibiting tandem catalysis has been firstly proposed. Recently, a new nanozyme based on a bionic zeolitic imidazolate framework-8 (ZIF-8) has the active center similar to hCAII, showing hCA as well as esterase and acetylcholinesterase-like activities. A new class of single-atom nanozymes, as FeN5 SA/CNF, with atomically dispersed enzyme-like active sites in nanomaterials has been discovered. The defined single-atom nanozymes provide a new perspective to the catalytic mechanism and rational design of nanozymes and exhibit great potential to become the next-generation nanozymes. References H. Wei, E. K. Wang, Chem. Soc. Rev., 2013, 42, 6060-6093. H. Wei, E. K. Wang, Anal. Chem., 2008, 80, 2250-2254. Q. Wang, X. Zhang, L. Huang, Z. Zhang, S. J. Dong, Angew. Chem. Int. Ed., 2017, 56, 16082. S. L. Rong, Y. C. Huang, J. W. Liu, E. K. Wang, H. Wei, Prog. Biochem. Biophys. 2018, 45, 129-147. Q. Wang, H. Wei, Z. Zhang, E. K. Wang, S. J. Dong, Trends Anal. Chem., 2018, 105, 218-224. WW. Wu, L. Huang, EK. Wang, SJ. Dong, Chem. Sci. 2020 11, 9741-9756. J. X. Chen, L. Huang, Q. Q. Wang, W. W. Wu, H. Zhang, Y. X. Fang, S. J. Dong, Nanoscale, 2019, 11, 5960-5966. L. Huang, J. X. Chen, L. F. Gan, J. Wang, S. J. Dong , Sci. Adv. 2019, 5, eaav5490. Acknowledgment This work was supported by the National Natural Science Foundation of China and The Ministry of Science and Technology of China All coworkers in this laboratory are appreciated for their effort in this area Key words Nanozymes, NiPd NPs, GOx@ZIF-8(NiPd) nanoflower, ZIF-8, acetylcholinesterase-like nanozyme , single-atom nanozyme- FeN5 SA/CNF
10
Khramtsov, Pavel, Maria Kropaneva, Maria Bochkova, Valeria Timganova, Dmitriy Kiselkov, Svetlana Zamorina, and Mikhail Rayev. "Synthesis and Application of Albumin Nanoparticles Loaded with Prussian Blue Nanozymes." Colloids and Interfaces 6, no. 2 (May 8, 2022): 29. http://dx.doi.org/10.3390/colloids6020029.
Full textAbstract:
Prussian blue nanozymes exhibit peroxidase-like catalytic activity and are therefore considered a stable and inexpensive alternative to natural peroxidases in the enzyme-linked immunosorbent assay (ELISA). In this work, we propose a robust method of Prussian blue nanozyme functionalization, which relies on the entrapment of nanozymes into albumin nanoparticles. The principle of the method is the addition of ethanol to a solution that contains albumin and nanozymes. At a high ethanol concentration solubility of albumin decreases, resulting in the formation of albumin nanoparticles loaded with nanozymes. The hydrodynamic diameter of nanoparticles was between 120 and 230 nm and depended on the nanozyme-to-BSA ratio. Encapsulation efficiency of nanozymes reached 96–99% and up to 190 μg of nanozymes were loaded per 1 mg of nanoparticles. Nanoparticles were stable at pH 5.5–7.5 and upon long-term storage in deionized water. Excellent reproducibility of the synthesis procedure was confirmed by the preparation of three individual batches of Prussian-blue-loaded BSA nanoparticles with almost identical properties. Nanoparticles were functionalized with monoclonal antibodies using glutaraldehyde cross-linking. The resulting conjugates were applied as labels in an ELISA-like assay of tumor marker prostate-specific antigen (PSA). The lower limit of detection was below 1 ng/mL, which enables measurement of PSA in the range of clinically relevant concentrations.
Dissertations / Theses on the topic "Nanozymes"
1
Mikolajczak, Dorian Jamal [Verfasser]. "Peptide-gold nanozymes as catalysts for green chemistry applications ranging from cascade catalysis to carbon capture / Dorian Jamal Mikolajczak." Berlin : Freie Universität Berlin, 2020. http://d-nb.info/1213294916/34.
Full text
2
Bonomi, Renato. "Catalizzatori idrolitici cooperativi: dai sistemi biomimetici ai nanozimi." Doctoral thesis, Università degli studi di Padova, 2010. http://hdl.handle.net/11577/3426893.
Full textAbstract:
This work aimed, firstly, at the production of hydrolitic catalysts, active towards organic phosphorous diesters. This was achieved through the cooperative interaction of metal ions and organic functional groups. Secondly, gold nanoparticles coated with metal-ions-bringing thiols were realized and used for catalisys.Realizzazione di catalizzatori idrolitici attivi nei confronti di diesteri fosforici organici mediante la cooperazione di ioni metallici e gruppi funzionali organici. In seconda istanza realizzazione di nanoparticelle d'oro ricoperte con tioli recanti complessi di ioni metallici per catalisi.
3
DONATI, PAOLO. "Colorimetric nanodiagnostics for Point-Of-Care applications: detection of salivary biomarkers and environmental contaminants." Doctoral thesis, Università degli studi di Genova, 2021. http://hdl.handle.net/11567/1046324.
Full textAbstract:
Nanomaterials offer many unique opportunities for the development of effective and rapid point-of-care (POC) devices to be exploited in many fields, including early diagnosis, health monitoring, and pollutant detection. In particular, gold nanoparticles (AuNPs) exhibit tunable catalytic and plasmonic properties, which are key enabling tools to design and develop innovative detection schemes in several sensing applications. The aim of this PhD project was the development of AuNPs-based colorimetric POCs to detect heavy metal ion contaminations and specific biomarkers in non-invasive biological fluids. First, we developed a novel strategy that exploits the combination of the plasmonic and catalytic properties of AuNPs to achieve an ultrafast (1 min) and sensitive colorimetric sensor for highly toxic methyl mercury. Taking advantage of the AuNP nanocatalyst to promote the rapid reduction of methyl mercury with nucleation on the particle surface and consequent aggregation-induced plasmonic shift, we were able to detect by naked-eye mercury contaminations as low as 20 ppb, which is relevant for food contaminations or biological fluid assessment. Moreover, an innovative and versatile platform, based on multibranched AuNPs, was developed for the detection of salivary biomarkers. Coupling etching and growing reactions in a reshaping process onto the nanostars surface, we created a customizable platform with boosted color change readout for fast detection of salivary glucose at low concentrations. The nanosensor performance was validated on samples from patients with diabetes, proving its potential as a novel non-invasive tool for frequent monitoring of glycaemia. As side project we also investigated the platinum nanoparticles enzymatic activity in a colorimetric sensor for inorganic mercury contamination monitoring in water sources.
4
Xu, Chang. "A Novel Mass Spectrometry Method to Study Reaction Intermediates and Development of AuTeCDs for Scavenging ROS in Live Cells." Ohio University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1597326111937675.
Full text
5
MASTRONARDI, VALENTINA. "Size- and shape-controlled platinum and palladium nanoparticles for catalytic and biomedical applications." Doctoral thesis, Università degli studi di Genova, 2021. http://hdl.handle.net/11567/1046749.
Full textAbstract:
The aim of this doctoral project has been centered on the development of innovative green synthetic methods able to govern the key physico-chemical properties of noble metal nanoparticles (NPs). To fully uncover the potential of metallic NPs, it is necessary to finely control the shape of nanomaterials while keeping the ultra-small characteristics, in order to achieve superior efficiency (per mass unit), selectivity and enhanced activity in catalytic processes. Size and shape of the nanomaterial together with the capping agents govern the surface properties and all the processes happening at the surface, such as catalysis. Different shapes offer great versatility to tune the nanocrystal (NCs) catalytic properties, which are dictated by surface facets. Green synthetic procedures have been developed to obtain pure, monodisperse, citrate-capped Pt and Pd NPs with accurate control on their size and the shape, without the use of polymers, surfactants and organic solvents. For Pd NPs, different geometrical shapes were achieved, such as icosahedrons, cubes, rods and wires while maintaining the thickness of 7 nm and length ranging from 38 to 470 nm (in the case of rods and wires) and the size (in the case of cubes and icosahedron) below 10 nm. These engineered nanomaterials exhibited good biocompatibility along with interesting enzymatic and catalytic properties, due to the absence of sticky molecules, high quality of the surface and the removal of toxic reagents. Moreover, a green synthetic procedure has been developed to obtain ultra-small Pt NCs by combining a strong and a weak reducing agent in aqueous environment in a single reaction vessel in only 10 minutes. NCs with size as low as 2.8 nm and high percentage of {111} surface domains have been achieved. These NCs have been physico-chemically and electrochemically characterized, disclosing significant perspectives for their use as innovative electrocatalysts or nanozymes in portable diagnostics.
6
Vernekar, Amit A. "Bio-inspired Materials : Antioxidant and Phosphotriesterase Nanozymes." Thesis, 2014. http://etd.iisc.ac.in/handle/2005/3026.
Full textAbstract:
Bio-inspired or biomimetic chemistry deals with the replication of the nature’s fundamental processes, which can help in understanding the functioning of biological systems and develop novel applications. Although a large number of researchers worked towards the replication of natural synthetic pathways through biogenetic syntheses, enzyme mimicry by the small organic molecules and inorganic complexes emerged in leaps and bounds over the years. The development of biomimetic chemistry then continued in designing the molecules that can function like enzymes. And now, with the advent of nanotechnology, nanostructured materials have been shown to exhibit enzyme-like activities (nanozymes). Interestingly, the two distinct fields, biology and materials science, have been integrated to form an entirely new area of research that has captured a great attention. Along with the pronounced application of nanomaterials as drug delivery vehicles, anticancer agents, antimicrobials, etc., research is also focused on designing nanomaterials for the biomimetic applications.
The thesis consists of five chapters. The first chapter provides a general overview of the recently discovered nanozymes that mimic heme-peroxidase, oxidase, superoxide dismutase, catalase, haloperoxidase and phosphatase. This chapter also deals with the nanozymes’ application in sensing and immunoassay, and as antioxidants, neuroprotective agents. The factors affecting the nanozymes’ activity and the challenges associated with them is also covered in this chapter. Chapter 2 is divided into two parts and it deals with the biomimetic properties of graphene-based materials. In part A, the remarkable peroxynitrite (PN) reductase and isomerase activities of hemin-functionalized reduced graphene oxide (rGO) is discussed. In part B, the activity of graphene oxide (GO) as peroxide substrate for the glutathione peroxidase (GPx) enzyme is discussed. In chapter 3, the oxidant material, V2O5, is shown to exhibit significant GPx-like antioxidant activity in its nano-form. Chapter 4 deals with the oxidase-like activity of MnFe2O4 nanooctahedrons for the antibody-free detection of major oxidative stress biomarker, carbonylated proteins. In chapter 5, the phosphotriesterase mimetic role of vacancy engineered nanoceria is discussed. instead of H2O2 for glutathione peroxidase (GPx) enzyme. As partial reduction of GO was observed when treated with GPx enzyme due to the fact that large sheet-like structures cannot be accessible to the active site, we studied the reaction with some GPx mimetics (Fig. 2). Varying the concentration of cofactor glutathione (GSH) required for the reaction, GPx mimic, ditelluride, could accomplish the reduction of GO following Michaelis-Menten kinetics. As the structure of GO is elusive and under active investigation, our study highlights the presence of peroxide linkages as integral part of GO other than hydroxyl, epoxy and carboxylic groups. This study also highlights an important fact that the modification of GO by biologically relevant compounds such as redox proteins must be taken into account when using GO for biomedical applications because such modifications can alter the fundamental properties of GO.
Figure 2. The GO reductase and decarboxylase activities of GPx mimetic ditelluride compound, suggesting the presence of peroxide linkages on GO.
In chapter 3, we have discussed about the novel antioxidant nanozyme that combats oxidative stress. During our attempts in the investigation of antioxidant nanozymes, we surprisingly noticed that the oxidant material, V2O5, shows significant GPx-like antioxidant activity in its nano-form. The Vn readily internalize in the cells and exhibit remarkable protective effects when challenged against reactive oxygen species (ROS). Although Vn has been shown to protect cells from ROS-induced damage, cells treated with bulk V2O5 and few vanadium complexes resulted in generation of ROS and severe toxicity. Detailed investigation on the mechanism of this interesting phenomenon
Chapter 4 deals with the development of novel methodology for detection of biomarkers. Inspired by the use of antibodies and enzymes for detection of a specific antigen, we have shown for the first time that the nanozymes can entirely replace antibodies and enzymes in Enzyme-linked Immunosorbent Assays (ELISA). As a specific example, we focused on the antibody-free detection of chief oxidative stress biomarker, carbonylated proteins, as our target. To achieve this, we designed MnFe2O4 nanooctahedrons that can function as oxidase enzyme and form signaling point of detection. We functionalized MnFe2O4 nanooctahedrons with hydrazide terminating groups so that carbonylated proteins can be linked to nanozymes by hydrazone linkage (Fig. 4a). Treatment of various carbonylated proteins (hemoglobin (Hb), Myoglobin (Mb), Cytochrome c (Cyt c), RNase and BSA) coated in well plate with hydrazide-terminated MnFe2O4 nanooctahedrons and then with 3,3’,5,5’-tetramethylbenzidine substrate, resulted in instantaneous detection by well plate reader (Fig. 4b). Considering the challenges and difficulties associated with the conventional methods used to detect such modified proteins, this methodology opens up a new avenue for the simple, cost-effective, instantaneous and entirely antibody-free ELISA-type detection of carbonylated proteins. Our results provide a cumulative application of nanozymes’ technology in oxidative stress associated areas and pave a new way for direct early detection of post translational modification (PTM) related diseases.
Figure 4. a) Nanozyme linked to the carbonylated protein coated on a plate through hydrazone linkage. b) General bar diagram showing detection of oxidized (carbonylated) proteins by nanozymes.
Synopsis
Figure 5. a) A cartoon view of surface of ceria showing vacancy. b) Zoomed portion of high resolution transmission electron microscopic image showing few vacancies on the surface of nanoceria. c) Catalytic mechanism of detoxification of paraoxon at the defect site.
In the final chapter, chapter 5, we have discussed about the nanomaterial that can function as phosphotriesterase enzyme. Phosphotriesterase enzyme is a bacterial enzyme that is involved in the rapid hydrolysis of sarin gas-related deadly nerve agents such as paraoxon, parathion and malathion. When encountered with these orgnaophospatetriesters, living beings tend to undergo nerve shock to cause paralysis by inhibiting an extremely important enzyme called acetylcholine esterase. They are also known to cause severe oxidative stress problems and are associated with neurodegenerative disorders. Therefore, curbing the toxic effects and detoxification of these nerve agents is a world-wide concern and many research teams have focused their attention to address this important problem. Working on the development of nanozymes for important problems, we found that nanoceria, especially the vacancy engineered one (Fig. 5a,b), can serve as active mimic of phosphotriesterase enzyme in the presence of N-methylmorpholine (acting as a distal base histidine). Vacancy engineered nanoceria has been shown to catalyze the hydrolysis of high amounts of paraoxon quiet efficiently and within few minutes with very low activation energy and high kcat. Detailed mechanistic investigation revealed that the presence of both Ce(III) and Ce(IV) is very essential for detoxification activity (Fig. 5b). The vacancies on the surface of nanoceria, were the buried Ce(III) ions are directly exposed to the reaction environment, behave as hotspots or enzyme active sites for detoxification reaction (Fig. 5b).
7
Vernekar, Amit A. "Bio-inspired Materials : Antioxidant and Phosphotriesterase Nanozymes." Thesis, 2014. http://hdl.handle.net/2005/3026.
Full textAbstract:
Bio-inspired or biomimetic chemistry deals with the replication of the nature’s fundamental processes, which can help in understanding the functioning of biological systems and develop novel applications. Although a large number of researchers worked towards the replication of natural synthetic pathways through biogenetic syntheses, enzyme mimicry by the small organic molecules and inorganic complexes emerged in leaps and bounds over the years. The development of biomimetic chemistry then continued in designing the molecules that can function like enzymes. And now, with the advent of nanotechnology, nanostructured materials have been shown to exhibit enzyme-like activities (nanozymes). Interestingly, the two distinct fields, biology and materials science, have been integrated to form an entirely new area of research that has captured a great attention. Along with the pronounced application of nanomaterials as drug delivery vehicles, anticancer agents, antimicrobials, etc., research is also focused on designing nanomaterials for the biomimetic applications.
The thesis consists of five chapters. The first chapter provides a general overview of the recently discovered nanozymes that mimic heme-peroxidase, oxidase, superoxide dismutase, catalase, haloperoxidase and phosphatase. This chapter also deals with the nanozymes’ application in sensing and immunoassay, and as antioxidants, neuroprotective agents. The factors affecting the nanozymes’ activity and the challenges associated with them is also covered in this chapter. Chapter 2 is divided into two parts and it deals with the biomimetic properties of graphene-based materials. In part A, the remarkable peroxynitrite (PN) reductase and isomerase activities of hemin-functionalized reduced graphene oxide (rGO) is discussed. In part B, the activity of graphene oxide (GO) as peroxide substrate for the glutathione peroxidase (GPx) enzyme is discussed. In chapter 3, the oxidant material, V2O5, is shown to exhibit significant GPx-like antioxidant activity in its nano-form. Chapter 4 deals with the oxidase-like activity of MnFe2O4 nanooctahedrons for the antibody-free detection of major oxidative stress biomarker, carbonylated proteins. In chapter 5, the phosphotriesterase mimetic role of vacancy engineered nanoceria is discussed. instead of H2O2 for glutathione peroxidase (GPx) enzyme. As partial reduction of GO was observed when treated with GPx enzyme due to the fact that large sheet-like structures cannot be accessible to the active site, we studied the reaction with some GPx mimetics (Fig. 2). Varying the concentration of cofactor glutathione (GSH) required for the reaction, GPx mimic, ditelluride, could accomplish the reduction of GO following Michaelis-Menten kinetics. As the structure of GO is elusive and under active investigation, our study highlights the presence of peroxide linkages as integral part of GO other than hydroxyl, epoxy and carboxylic groups. This study also highlights an important fact that the modification of GO by biologically relevant compounds such as redox proteins must be taken into account when using GO for biomedical applications because such modifications can alter the fundamental properties of GO.
Figure 2. The GO reductase and decarboxylase activities of GPx mimetic ditelluride compound, suggesting the presence of peroxide linkages on GO.
In chapter 3, we have discussed about the novel antioxidant nanozyme that combats oxidative stress. During our attempts in the investigation of antioxidant nanozymes, we surprisingly noticed that the oxidant material, V2O5, shows significant GPx-like antioxidant activity in its nano-form. The Vn readily internalize in the cells and exhibit remarkable protective effects when challenged against reactive oxygen species (ROS). Although Vn has been shown to protect cells from ROS-induced damage, cells treated with bulk V2O5 and few vanadium complexes resulted in generation of ROS and severe toxicity. Detailed investigation on the mechanism of this interesting phenomenon
Chapter 4 deals with the development of novel methodology for detection of biomarkers. Inspired by the use of antibodies and enzymes for detection of a specific antigen, we have shown for the first time that the nanozymes can entirely replace antibodies and enzymes in Enzyme-linked Immunosorbent Assays (ELISA). As a specific example, we focused on the antibody-free detection of chief oxidative stress biomarker, carbonylated proteins, as our target. To achieve this, we designed MnFe2O4 nanooctahedrons that can function as oxidase enzyme and form signaling point of detection. We functionalized MnFe2O4 nanooctahedrons with hydrazide terminating groups so that carbonylated proteins can be linked to nanozymes by hydrazone linkage (Fig. 4a). Treatment of various carbonylated proteins (hemoglobin (Hb), Myoglobin (Mb), Cytochrome c (Cyt c), RNase and BSA) coated in well plate with hydrazide-terminated MnFe2O4 nanooctahedrons and then with 3,3’,5,5’-tetramethylbenzidine substrate, resulted in instantaneous detection by well plate reader (Fig. 4b). Considering the challenges and difficulties associated with the conventional methods used to detect such modified proteins, this methodology opens up a new avenue for the simple, cost-effective, instantaneous and entirely antibody-free ELISA-type detection of carbonylated proteins. Our results provide a cumulative application of nanozymes’ technology in oxidative stress associated areas and pave a new way for direct early detection of post translational modification (PTM) related diseases.
Figure 4. a) Nanozyme linked to the carbonylated protein coated on a plate through hydrazone linkage. b) General bar diagram showing detection of oxidized (carbonylated) proteins by nanozymes.
Synopsis
Figure 5. a) A cartoon view of surface of ceria showing vacancy. b) Zoomed portion of high resolution transmission electron microscopic image showing few vacancies on the surface of nanoceria. c) Catalytic mechanism of detoxification of paraoxon at the defect site.
In the final chapter, chapter 5, we have discussed about the nanomaterial that can function as phosphotriesterase enzyme. Phosphotriesterase enzyme is a bacterial enzyme that is involved in the rapid hydrolysis of sarin gas-related deadly nerve agents such as paraoxon, parathion and malathion. When encountered with these orgnaophospatetriesters, living beings tend to undergo nerve shock to cause paralysis by inhibiting an extremely important enzyme called acetylcholine esterase. They are also known to cause severe oxidative stress problems and are associated with neurodegenerative disorders. Therefore, curbing the toxic effects and detoxification of these nerve agents is a world-wide concern and many research teams have focused their attention to address this important problem. Working on the development of nanozymes for important problems, we found that nanoceria, especially the vacancy engineered one (Fig. 5a,b), can serve as active mimic of phosphotriesterase enzyme in the presence of N-methylmorpholine (acting as a distal base histidine). Vacancy engineered nanoceria has been shown to catalyze the hydrolysis of high amounts of paraoxon quiet efficiently and within few minutes with very low activation energy and high kcat. Detailed mechanistic investigation revealed that the presence of both Ce(III) and Ce(IV) is very essential for detoxification activity (Fig. 5b). The vacancies on the surface of nanoceria, were the buried Ce(III) ions are directly exposed to the reaction environment, behave as hotspots or enzyme active sites for detoxification reaction (Fig. 5b).
8
Lien, Chia-Wen, and 連嘉文. "Synthesis of Nanozymes for Sensing of Proteins, Heavy Metal Ions, and Anions." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/8b7888.
Full textAbstract:
博士國立臺灣大學
化學研究所
105
Nanozymes can catalyze specific reactions. In addition, nanozymes possess unique features such as large surface area for further conjugation of multiple molecules for biorecognition. In this study, we synthesized nanozymes and applied them in the detection of biomolecules and heavy metal ions. This dissertation is structured in four chapters. In Chapter 1, the background of nanozymes including their classification, developments, and applications is reviewed. In Chapter 2, Bismuth–gold nanoparticles (Bi–Au NPs) were prepared through self-deposition of bismuth ions (Bi3+) on Au NPs as a result of their aurophilic interactions. Bi–Au NPs possess intrinsic peroxidase-like activity, which in the prescence of hydrogen peroxide (H2O2) could catalytically oxidize Amplex Red (AR) to produce highly fluorescent resorufin, applicable for the detection of H2O2. Then, the as-prepared Bi–Au NPs were further modified with fibrinogen (Fib) to form fibrinogen-adsorbed Bi–Au NPs (Fib-Bi–Au NPs). In the presence of thrombin, soluble fibrinogen converts into insoluble strands of fibrin on Bi–Au NPs'' surfaces, causing a decrease in peroxidase-like activity of Bi–Au NPs, which can be employed in the detection of thrombin and further screening of anticoagulant drugs for thrombin. In Chapter 3, we have successfully demonstrated that Au NPs can be programmed to regulate their peroxidase (POX)-, oxidase (OX)- and catalase (CAT)-like activities through deposition of various metal ions (Ag+, Bi3+, Pb2+, Pt4+, Hg2+). Furthermore, we used metal ions (i.e., Hg2+/Bi3+, Pt4+/Hg2+, Pb2+/Hg2+, and Ag+/Bi3+, respectively) as inputs and the enzyme-like activity of the Au NPs as the output for the construction of OR, AND, INHIBIT, and XOR logic gates. In Chapter 4, we used as constructed “Pt4+/Pb2+(AND)Au NPPOX” and “Bi3+/Hg2+(INHIBIT)Au NPPOX” logic gates for the selective detection of Pb2+ and Hg2+. When Pt4+ and Pb2+ co-exist, strong metallophilic interactions (between Pt and Pb atoms/ions) and aurophilic interactions (between Au and Pb/Pt atoms/ions) result in significant enhanced peroxidase-like activity (AND logic gate) which were then employed in the detection of Pb2+. High peroxidase-like activity of Au NPs in the presence of Bi3+ is a result of the various valence (oxidation) states of Bi3+ and Au (Au+/Au0) atoms on the nanoparticle’s surface. When Bi3+ and Hg2+ co-exist, strong Hg–Au amalgamation results in a large decrease in the peroxidase-like activity (INHIBIT logic gate) of the Au NPs which were then employed in the detection of Hg2+. In addition, an integrated logic circuit based on the color change (formation of reddish resorufin product) and generation of O2 bubbles from these two probes has been constructed, allowing visual detection of Pb2+ and Hg2+ in aqueous solution. In Chapter 5, we used a simple one-step synthesis of well-dispersed amorphous cobalt hydroxide/oxide-modified graphene oxide (CoOxH-GO) possessing peroxidase-like catalytic activity. Interestingly, cyanide ions (CN–) significantly inhibited the catalytic activity of CoOxH-GO nanocomposite, which allows for the construction of a probe for the detection of CN– in water samples and laboratory wastes. We fabricated a paper-based CoOxH-GO probe for the visual detection of CN– by preparing a thin film of CoOxH-GO on a positively charged and porous nylon membrane (N+M), which operates on the principle that CN– inhibited CoOxH-GO catalyzed H2O2 mediated oxidation of AR to reddish resorufin on membrane. The intensity of the red color of membrane decreases with the increase in CN– concentration, which can be easily observed with naked eye in nanomolar concentrations.
9
Singh, Namrata. "Development of Nanomaterials as Antioxidant Enzyme Mimetics for Cellular Redox Homeostasis." Thesis, 2018. https://etd.iisc.ac.in/handle/2005/5344.
Full textAbstract:
Nanomaterials possess a myriad of potential in various biomedical fields such as cancer diagnostics, biosensing, imaging, immunoassay, drug delivery and therapeutics etc. In particular, the ability of the nanozymes to modulate the catalytic activities and biological functions of natural enzymes attracts burgeoning interest for various biomimetic applications. There is a significant relevance of nanozymes which can regulate the reactive oxygen species (ROS) levels in cells, mimicking the cellular antioxidant enzymes due to their possible therapeutic potential in various oxidative stress related disorders. However, there are certain drawbacks associated with their selectivity, limited surface area due to functionalization and biocompatibility. Further, the mechanistic insights revealing the enzymatic role of nanozymes in a cellular environment are not well explored. Our study introduces novel enzyme-mimetics as artificial antioxidants for further development as a potential therapy against oxidative stress-mediated pathological conditions.
10
Kuo, Po-Chih, and 郭柏志. "Characterization of spermine oxidase in zebrafish and developing a method to detect spermine in biological samples by using silver-gold/silver chloride nanozymes." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/4e4cc9.
Full textBooks on the topic "Nanozymes"
1
Gunasekaran, Sundaram. Nanozymes. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228.
Full text
2
Daima, Hemant Kumar, Navya PN, and Eric Lichtfouse, eds. Nanozymes in Medicine. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-20581-1.
Full text
3
Daima, Hemant Kumar, Navya PN, and Eric Lichtfouse, eds. Nanozymes for Environmental Engineering. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-68230-9.
Full text
4
Wang, Xiaoyu, ed. Nanozymes: Design, Synthesis, and Applications. Washington, DC: American Chemical Society, 2022. http://dx.doi.org/10.1021/bk-2022-1422.
Full text
5
Wang, Xiaoyu, Wenjing Guo, Yihui Hu, Jiangjiexing Wu, and Hui Wei. Nanozymes: Next Wave of Artificial Enzymes. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-53068-9.
Full text
8
Gunasekaran, Sundaram. Nanozymes: Advances and Applications. Taylor & Francis Group, 2021.
Find full text
9
Daima, Hamant Kumar, Eric Lichtfouse, and Navya PN. Nanozymes for Environmental Engineering. Springer International Publishing AG, 2021.
Find full text
10
Lichtfouse, Eric, Hemant Kumar Daima, and Navya PN. Nanozymes for Environmental Engineering. Springer International Publishing AG, 2022.
Find full textBook chapters on the topic "Nanozymes"
1
Erkmen, Cem, Sevinc Kurbanoglu, and Bengi Uslu. "Nanozymes in Electrochemical Analysis." In Nanozymes, 159–98. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-9.
Full text
2
Li, Xin, Peng Liu, Mengzhu Wang, and Xiangheng Niu. "Nanozymes in Detecting Environmental Pollutants." In Nanozymes, 225–51. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-11.
Full text
3
Wang, Xianwen, Xiaoyan Zhong, Haisheng Qian, and Zhengbao Zha. "Nanozymes as Photothermal-Catalytic Agents." In Nanozymes, 199–224. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-10.
Full text
4
Luo, Mai, Ting Wang, Ling Chen, Zehua Cheng, Sundaram Gunasekaran, Jinchao Wei, and Peng Li. "Nanozymes in Pesticides Detection." In Nanozymes, 253–73. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-12.
Full text
5
Ahmed, Syed Rahin, Ana Gomez Cardoso, Satish Kumar, Greter A. Ortega, Seshasai Srinivasan, and Amin Reza Rajabzadeh. "Nanozymes in Biosensing and Bioimaging." In Nanozymes, 115–43. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-7.
Full text
6
Wang, Weizheng, and Sundaram Gunasekaran. "Potential Toxicology of Nanozymes." In Nanozymes, 377–401. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-17.
Full text
7
Liu, Biwu, and Juewen Liu. "Metal Oxide Nanozymes." In Nanozymes, 29–46. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-3.
Full text
8
Kip, Çiğdem, Kadriye Özlem Hamaloğlu, and Ali Tuncel. "Bioaffinity-Based Nanozymes." In Nanozymes, 97–113. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-6.
Full text
9
Alaei, Loghman, Zhila Izadi, Samira Jafari, Alireza Lotfabadi, Ebrahim Barzegari, Mehdi Jaymand, and Hossein Derakhshankhah. "Nanozymes—An Overview." In Nanozymes, 15–28. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-2.
Full text
10
Singh, Smriti, and Seema Nara. "Gold Nanozymes in Therapeutics." In Nanozymes, 145–57. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003109228-8.
Full textConference papers on the topic "Nanozymes"
1
Costa, Lúcia A. A., Marta Mateus, João Paulo Borges, Jorge Carvalho Silva, Susana Barreiros, and Paula I. P. Soares. "Superparamagnetic Iron Oxide Nanozymes for Synergistic Cancer Treatment." In Materiais 2022. Basel Switzerland: MDPI, 2022. http://dx.doi.org/10.3390/materproc2022008003.
Full text
2
Pecina, Adam, Paolo Scrimin, Fabrizio Mancin, and Marco De Vivo. "Mechanistic Insight into the Phosphodiester Bond Hydrolysis of Nanozymes." In 5th International Conference on Theoretical and Applied Nanoscience and Nanotechnology (TANN'21). Avestia Publishing, 2021. http://dx.doi.org/10.11159/tann21.115.
Full text
3
Hu, Xinyu. "GO-PdNi composite nanozyme for detection of ascorbic acid." In 2nd International Conference on Testing Technology and Automation Engineering (TTAE 2022), edited by Yang Yue. SPIE, 2022. http://dx.doi.org/10.1117/12.2660644.
Full text
4
Othman, Ali, Akhtar Hayat, and Silvana Andreescu. "Europium-Doped Ceria Nanocrystals as Nanozyme Fluorescent Probes for Biosensing." In CSAC2021. Basel Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/csac2021-10549.
Full text
5
Dinu, Livia Alexandra, Angela Mihaela Baracu, and Oana Brincoveanu. "The non-enzymatic detection of the pollutant bisphenol A using S-graphene as nanozyme material." In 2022 International Semiconductor Conference (CAS). IEEE, 2022. http://dx.doi.org/10.1109/cas56377.2022.9934301.
Full text
6
Pandey, Indu, and Jai Deo Tiwari. "Shape-printed nanozyme coated wet tissue paper based sensor for electrochemical sensing of 8-Hydroxy-2’ -deoxyguanosine." In 2020 International Conference on Electrical and Electronics Engineering (ICE3). IEEE, 2020. http://dx.doi.org/10.1109/ice348803.2020.9122854.
Full text
7
Bansal, Payal, Mohammad Javed Ansari, Maruthi Rohit Ayyagari, Ramji Kalidoss, Abhishek Madduri, and Rahul Kanaoujiya. "Carbon quantum dots based nanozyme as bio-sensor for enhanced detection of glutathione (U) from cancer cells." In PROCEEDING OF INTERNATIONAL CONFERENCE ON ENERGY, MANUFACTURE, ADVANCED MATERIAL AND MECHATRONICS 2021. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0126126.
Full text
8
Kulah, Jonathan, and Ahmet Aykaç. "Synthesis and Characterization of Graphene Quantum Dots Functionalized Silver Nanoparticle from Moringa Oleifera Extracts." In 6th International Students Science Congress. Izmir International Guest Student Association, 2022. http://dx.doi.org/10.52460/issc.2022.050.
Full textAbstract:
Graphene quantum dots (GQDs) are famously known for large surface area, good dispersibility, good conductivity, and high transparency with good photochemical, electrochemical, and optical properties that are utilized in many biomedical and biotechnological applications. Interestingly, GQDs were reported to serve as an excellent reducing reagent in the synthesis of noble metal nanoparticles such as silver nanoparticles (AgNPs). Moreover, GQDs eradicate the limitation of impurities of AgNPs synthesized using plant extracts as a stabilizer and reducing agents. Therefore, we experimented GQDs synthesis from moringa oleifera (MO) plant extracts compared to citric and urea synthesized GQDs. And used the synthesized GQDs to synthesize, reduce and functionalize AgNPs. MO contains about 110 compounds, high nutrients, vitamins, oleic oil, and phytoconstituents such as alkaloids, flavonoids, glucosinolates, saponins, tannins, terpenes, steroids, phenolic acids, which suggested to us that, MO extracts can serve as a capping agent in the synthesis of nanoparticles. Initially, MO leaves and seeds water phase extracts were obtained by overnight distillation and lyophilized to create a stock solution of 1mg/ml. Next, following Das, R. et al and slightly modifying the followed method by varying the MO extract concentration from 20µL to 60 µL, AgNPs were synthesized by hydrothermal method. GQDs were separately synthesized adopting Tran, H.V. et al method and later added to the AgNPs forming a more stable hybrid structure that was characterized using the UV-vis spectroscopy (UV-Vis), Nano zeta sizer, Raman spectroscopy, and the Fourier Infrared transmission resonance (FTIR). As the concentration of MO extract increased, the color change intensity increased symbolizing the formation of AgNPs while the luminous bright solutions under the UV light symbolized the formation of GQDs. This study lay the foundation for further research and analysis to be done on the nanozyme or biosensor application of enhanced functionalized and stable hybrid AgNPs with GQDs.