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1

Sayre, Jeff. "Propagation Protocol for American Lotus ( Nelumbo lutea Willd.)." Native Plants Journal 5, no. 1 (2004): 14–17. http://dx.doi.org/10.1353/npj.2004.0017.

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2

Schubert, Peter, Jutta Lorenz, and Dirk Ullrich. "Lotosblumen (Nelumbo Adans.) im heimischen Gartenteich?" Der Palmengarten 66, no. 2 (2018): 126–35. http://dx.doi.org/10.21248/palmengarten.391.

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Der indische (Nelumbo nucifera) und der amerikanische Lotos (Nelumbo lutea) sind außerordentlich attraktive Wasserpflanzen. Nelumbo nucifera hat hohen Symbolwert in den südostasiatischen Kulturen, wird in Asien aber auch als Nahrungspflanze sehr geschätzt. Die Oberfläche der Blätter hat wichtige Hinweise zur Entwicklung selbstreinigender Oberflächen von Kunststoffen und Lacken geliefert. Weil für die Blütenentwicklung Sommertemperaturen über 20 °C notwendig sind, ist Lotos im Freiland mitteleuropäischer Gärten kaum zu finden. Wenn man jedoch bestimmte Kulturmaßnahmen beachtet, ist seine Freilandkultur in sommerwarmen Gebieten Deutschlands durchaus zu empfehlen. Davon konnte man sich in den letzten Jahren im Botanischen Garten in Mainz und im Palmengarten in Frankfurt überzeugen.
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3

Dieringer, Gregg, R. Leticia Cabrera, and Mohammad Mottaleb. "Ecological relationship between floral thermogenesis and pollination in Nelumbo lutea (Nelumbonaceae)." American Journal of Botany 101, no. 2 (2014): 357–64. http://dx.doi.org/10.3732/ajb.1300370.

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4

Fu, Jie, Qiaoyan Xiang, Xianbao Zeng, Mei Yang, Ying Wang, and Yanling Liu. "Assessment of the Genetic Diversity and Population Structure of Lotus Cultivars Grown in China by Amplified Fragment Length Polymorphism." Journal of the American Society for Horticultural Science 136, no. 5 (2011): 339–49. http://dx.doi.org/10.21273/jashs.136.5.339.

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To assess the genetic diversity among lotus (Nelumbo) accessions and evaluate the correlation between genetic variation and morphological classification, we sampled 138 accessions: two of N. lutea, 112 of N. nucifera, 17 of hybrids between N. nucifera and N. lutea, and seven Japanese cultivars. The 11 selected combinations of amplified fragment length polymorphism (AFLP) primers produced 138 polymorphic loci, and the percentage of polymorphism was 28.7%. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram clustered all the accessions into two groups: Group I comprised N. lutea and its hybrids with N. nucifera; Group II included N. nucifera and its hybrids with N. lutea and Japanese cultivars. Population structure analysis identified four main clusters: N. lutea clustered mainly in C1, whereas N. nucifera clustered in C2, C3, and C4, which was consistent with the UPGMA and principal coordinate analysis results. The Japanese cultivars were related more closely to N. nucifera (genetic similarity coefficient = 0.74) than to N. lutea (0.46); hence, the Japanese cultivars can be classified as N. nucifera. Moreover, rhizome lotuses formed a separate subclade, whereas seed lotuses were interspersed among flower lotuses, which demonstrated that rhizome lotuses were distinct from flower and seed lotuses. Plant size, flower color, and other morphological criteria used commonly to classify lotuses were correlated with genetic variation to a certain extent but not sufficiently for accurate classification. It appears that it is necessary to use both DNA markers and morphological characteristics to classify lotus species and cultivars.
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5

Francko, David A. "Studies on Nelumbo lutea (Willd.) pers. I. Techniques for axenic liquid seed culture." Aquatic Botany 26 (January 1986): 113–17. http://dx.doi.org/10.1016/0304-3770(86)90009-4.

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6

Tian, Daike, Ken M. Tilt, Jeff L. Sibley, Fenny Dane, and Floyd M. Woods. "Response of Lotus (Nelumbo sp.) to Container Soil Volume." Journal of Environmental Horticulture 27, no. 2 (2009): 79–84. http://dx.doi.org/10.24266/0738-2898-27.2.79.

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Abstract The effect of soil volume on containerized lotus (Nelumbo) production has been underreported. American lotus (Nelumbo lutea Willd.) and three cultivars (‘Embolene’, ‘98 Seed’ and ‘Karizma’) of Asian lotus (N. nucifera Gaertn.) were investigated for growth response to container soil volume in this study. Electrical conductivity, pH, plant growth indices, and plant nutritional content were influenced by container soil volume. Differences in some plant growth indices were significant between treatments with ½ and higher (½ and ¾) container height soil (CHS) in 21 or 29 liter (#5 or #7) containers. However, plant growth indices were generally not different between treatments with ½ and ¾ CHS. Lotus planted in containers with ¼ CHS usually produced the greatest plant height and underground fresh weight, while the largest number of propagules often occurred in containers with ½ or ¾ CHS. The highest number of emerging leaves was observed in plants with ¼ or ½ CHS treatments, with no significant difference in emerging leaf number between lotus grown in containers with ½ and ¾ CHS. Flower number generally decreased as soil level increased. The ¼ and ½ CHS were more efficient than ¾ CHS for lotus production in containers.
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7

Kubo, Nakao, Masashi Hirai, Akio Kaneko, Daizo Tanaka, and Kumaji Kasumi. "Classification and diversity of sacred and American Nelumbo species: the genetic relationships of flowering lotus cultivars in Japan using SSR markers." Plant Genetic Resources 7, no. 03 (2009): 260–70. http://dx.doi.org/10.1017/s1479262109356580.

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The water lotus, genusNelumbo, contains two species, the sacred (Nelumbo nucifera) and American lotuses (Nelumbo lutea). Hundreds of flowering lotus cultivars are currently known. However, their classification is unclear. For the classification ofNelumbocultivars, in addition to 35 simple sequence repeat (SSR) markers recently developed, we have developed 17 and 16 of newNelumboSSR markers from SSR-enriched genomic libraries and expressed sequence tag (EST) data, respectively. Out of these 68 SSRs, along with SSRs recently published by others, 52 showed clear polymorphisms in 98Nelumbosamples. A total of 300 alleles were observed, ranging from 2 to 11 alleles per locus, with an average of 5.77. Alleles specific for the American lotus-derived cultivars and a cluster of the American lotus-derived cultivars on a neighbour-joining tree confirmed genetic differences betweenN. luteaandN. nucifera. In addition, a possible differentiation between Chinese and Japanese cultivars was also suggested. Parentage analysis using the SSR markers confirmed four known parentages and predicted currently-unknown parentages of six cultivars. The present data have demonstrated that site-specific, co-dominant SSR markers enable more accurate classification, identification and comparison ofNelumbospecies.
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8

Zhang, Dasheng, Qing Chen, Qingqing Liu, et al. "Histological and Cytological Characterization of Anther and Appendage Development in Asian Lotus (Nelumbo nucifera Gaertn.)." International Journal of Molecular Sciences 20, no. 5 (2019): 1015. http://dx.doi.org/10.3390/ijms20051015.

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The lotus (Nelumbo Adans.) is a perennial aquatic plant with important value in horticulture, medicine, food, religion, and culture. It is rich in germplasm and more than 2000 cultivars have been cultivated through hybridization and natural selection. Microsporogenesis and male gametogenesis in the anther are important for hybridization in flowering plants. However, little is known about the cytological events, especially related to the stamen, during the reproduction of the lotus. To better understand the mechanism controlling the male reproductive development of the lotus, we investigated the flower structure of the Asian lotus (N. nucifera). The cytological analysis of anther morphogenesis showed both the common and specialized cytological events as well as the formation of mature pollen grains via meiosis and mitosis during lotus anther development. Intriguingly, an anatomical difference in anther appendage structures was observed between the Asian lotus and the American lotus (N. lutea). To facilitate future study on lotus male reproduction, we categorized pollen development into 11 stages according to the characterized cytological events. This discovery expands our knowledge on the pollen and appendage development of the lotus as well as improving the understanding of the species differentiation of N. nucifera and N. lutea.
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9

Swan, Daniel C. "The North American Lotus (Nelumbo lutea Willd Pers.) - Sacred Food of the Osage People." Ethnobotany Research and Applications 8 (November 23, 2010): 249. http://dx.doi.org/10.17348/era.8.0.249-253.

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10

Francko, David A. "Studies on Nelumbo lutea (Willd.) Pers. II. Effects of pH on photosynthetic carbon assimilation." Aquatic Botany 26 (January 1986): 119–27. http://dx.doi.org/10.1016/0304-3770(86)90010-0.

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11

Islam, Md Rabiul, Ying Zhang, Zhi-Zhong Li, Hong Liu, Jin-Ming Chen, and Xing-Yu Yang. "Genetic diversity, population structure, and historical gene flow of Nelumbo lutea in USA using microsatellite markers." Aquatic Botany 160 (January 2020): 103162. http://dx.doi.org/10.1016/j.aquabot.2019.103162.

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12

Li, Chun, Hai-bo Mo, Dai-ke Tian, Yu-xian Xu, Jing Meng, and Ken Tilt. "Genetic diversity and structure of American lotus (Nelumbo lutea Willd.) in North America revealed from microsatellite markers." Scientia Horticulturae 189 (June 2015): 17–21. http://dx.doi.org/10.1016/j.scienta.2015.03.026.

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13

Al-Hamdani, Safaa, and David A. Francko. "Effect of light and temperature on photosynthesis, elongation rate and chlorophyll content of Nelumbo lutea (Willd.) Pers. seedlings." Aquatic Botany 44, no. 1 (1992): 51–58. http://dx.doi.org/10.1016/0304-3770(92)90080-3.

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14

Ishizuna, Fumiko, and Nobuhiro Tsutsumi. "Flower Bud Formation of Sacred Lotus (Nelumbo nucifera Gaertn.): A Case Study of ‘Gyozankouren’ Grown in a Container." HortScience 49, no. 4 (2014): 516–18. http://dx.doi.org/10.21273/hortsci.49.4.516.

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The genus Nelumbo consists of two species, N. nucifera and N. lutea. N. nucifera is an ornamental and edible plant that is widely cultivated. Earlier studies of sacred lotus (N. nucifera) flowers focused mainly on morphology, phyllotaxis, leaf arrangements, and flower development. During the growing season, sacred lotus produces one foliage leaf at each node. Flower buds emerge from the abaxial side of the basal part of the foliage leaf. However, the number of blooming flowers is much less than the number of foliage leaves. Little is known concerning flower bud formation during lotus plant development. This is the first experimental study to reveal that every node has one flower bud even in the dormant shoot apex and that most of the formed flower buds aborted in the course of floral development. Our results suggest that flower bud formation of sacred lotus is independent of daylength. On the other hand, whether a formed bud reaches blooming may depend on environmental factors.
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15

Lee, Jessica F., Francis E. Durbian, Ron Bell, and Michael Voltz. "Pied-Billed Grebe Nesting Ecology on Squaw Creek National Wildlife Refuge,1996–2007." Transactions of the Missouri Academy of Science 42, no. 2008 (2008): 23–29. http://dx.doi.org/10.30956/0544-540x-42.2008.23.

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Pied-billed Grebes (Podilymbus podiceps) are secretive water-birds that build floating nests in smaller wetlands. Our study focused on the nesting ecology, including phenology, of Pied-billed Grebes at Squaw Creek National Wildlife Refuge in northwest Missouri during an 11 year period. We located 956 Pied-billed Grebe nests in wetlands from April to July 1996–2007 and documented the number of eggs in the nest, water depth at the nest, and surrounding vegetation. Using this information, the mean clutch size, mean nest initiation date, mean hatching date, mean water depth at nesting sites, and most common vegetation types surrounding nests were determined and analyzed for differences among years. The mean clutch size for all nests was 7.18 eggs/nest (SE = 0. 09, n = 249) although clutch size significantly varied among years (F=3.51, df=7, P=0.0013). Mean nest initiation date from 414 nests was 18 May while the mean hatching date was 11 June. Both dates were significantly different among years (F=31.86, df=11, P<0.0001 and F=31.93, df=11, P<0.0001). Mean water depth at nest sites was 62.03 cm (SE=0.45, n=561) and varied significantly across years (F=46.50, df= 7, P<0.0001). River bulrush (Schoenoplectus fluviatilis) was the most common vegetation type found at 72.2% of 573 nesting sites followed by arrowhead (Sagitarria latifolia) (23.2%), smartweed (Polygonum sp.) (20.8%), and American lotus (Nelumbo lutea) (19.5%). All Pied-billed Grebe nesting data from Squaw Creek NWR falls within the ranges reported in other nesting studies. It appears current management on the refuge is adequate to support quality Pied-billed Grebe nesting habitat.
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