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Journal articles on the topic 'Neon electroporation system'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Molla, Shahan, Lauren Gentles, Bindhu Hosuru, Namritha Ravinder, and Arseny Smirnov. "Abstract LB362: Optimizing non-viral genome editing workflow for primary activated T cells with advanced electroporation technology." Cancer Research 85, no. 8_Supplement_2 (2025): LB362. https://doi.org/10.1158/1538-7445.am2025-lb362.

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Abstract CAR-T therapy has shown remarkable success in treating hematologic cancers such as leukemia and lymphoma. The Neon™ NxT Electroporation System with 8-Channel Pipette is a powerful tool for streamlined optimization of electroporation programs and other electroporation conditions for primary cells and cell lines. Here, we showcase the system's performance in efficiently optimizing CRISPR/Cas9 based non-viral genome editing of primary activated T cells.Using the Neon™ NxT Electroporation System with 8-Channel Pipette, we generate CAR-T cells by delivering CD19 donor DNA, a TRAC gRNA, and

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2

Chang, Sean, Nektaria Andronikou, and Xavier de Mollerat du Jeu. "Optimization of Gene Editing in Human Primary T Cells with Neon® Transfection System." Journal of Immunology 198, no. 1_Supplement (2017): 73.24. http://dx.doi.org/10.4049/jimmunol.198.supp.73.24.

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Abstract The successful cases of autologous CAR-T cell therapy in leukemia have highlighted the promising future of cell therapy. However, most studies in this field have been using viruses to engineer T cells, which lead to safety concerns. On the other hand, there has been a growing interest in developing allogeneic immune cell therapies to tackle the challenges of production scale up. Hence, there is a need for a better delivery system to maximize gene editing efficiency in immune cells. Neon® Transfection System has been widely used in a variety of cell models and especially hard-to-transf

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3

Chang, Sean, Xin Yu, Yongchang Ji, Xiquan Liang, Nektaria Andronikou, and Xavier de Mollerat du Jeu. "Non-viral and viral delivery solutions for next generation cell therapy." Journal of Immunology 200, no. 1_Supplement (2018): 179.14. http://dx.doi.org/10.4049/jimmunol.200.supp.179.14.

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Abstract The successes of chimeric antigen receptor (CAR) T cells in treating blood cancers have highlighted the cell therapy era. However, the difficulty of delivering molecules into immune cells has been an obstacle to more rapid advancement. Here we present an innovative large-scale Lentivirus (LV) production system as a solution to lower the cost and time of viral production. On the other hand, the next generation cell therapy will rely heavily on gene editing, especially in a safer non-viral integration manner. We have demonstrated that our novel non-viral all-in-one electroporation metho

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4

Dey, Isha, Xiaoyu Jenny Yang, Jacob Delgadillo, et al. "Abstract 5193: Optimization of tumoroid size and payload delivery methods for the engineering of patient-derived tumoroid models." Cancer Research 85, no. 8_Supplement_1 (2025): 5193. https://doi.org/10.1158/1538-7445.am2025-5193.

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Abstract Tumoroids, also known as cancer organoids, are three-dimensional (3D) in vitro cell cultures established from primary tissue samples. These next-generation cancer cell models largely recapitulate the spatial organization, gene expression, and mutational profiles of patient tumors. It may be beneficial to engineer tumoroids to generate reporter lines, model specific mutations involved with tumor progression, or modulate gene expression to help understand the mechanism of action of therapeutics. Here, we explore methods for genetic engineering of tumoroids established and cultured in Gi

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5

Erwin, Nina, Xiaoshu Pan, Nikee Awasthee, et al. "Abstract LB002: Extracellular vesicle loading of proteolysis targeting chimeras for targeted therapeutic delivery." Cancer Research 83, no. 8_Supplement (2023): LB002. http://dx.doi.org/10.1158/1538-7445.am2023-lb002.

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Abstract Breast cancer is a significant public health issue that remains the second leading cause of cancer death among women. Current treatment strategies for breast cancer can contribute to severe side effects and insufficient efficacy. For example, chemotherapy, radiotherapy, and hormone receptor and HER2 targeted therapies can lead to healthy cell damage, a partial, delayed effect, and cardiac toxicity and acquired immune resistance, respectively. An emerging alternative targeted therapy approach is proteolysis targeting chimeras (PROTAC), which triggers proteasomal degradation of target p

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6

Basar, Rafet, May Daher, Nadima Uprety, et al. "Next Generation CRISPR Gene-Edited and Off-the-Shelf Virus-Specific T-Cells for the Immunocompromised Patient." Blood 134, Supplement_1 (2019): 1944. http://dx.doi.org/10.1182/blood-2019-125276.

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Introduction: A number of Clinical trials have demonstrated the feasibility, safety and efficacy of cell and gene therapy for cancer, autoimmune disorders and infectious disease. Strategies that enhance the function and survival of immune cells are critical for the success of immunotherapy. We have developed a strategy for the ex vivo expansion of off-the-shelf viral-specific T cells (VSRs) from healthy donor buffy coat which have been extremely effective in eradicating refractory cytomegalovirus (CMV), polyomavirus and adenovirus infections in immunocompromised patients. Glucocorticoids commo

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7

Kusanagi, A., J. Yamasaki, C. Iwatani, H. Tsuchiya, and R. Torii. "220 NONHUMAN PRIMATE EMBRYONIC STEM CELLS SIMILAR TO THE BIOLOGICAL PROPERTIES OF MOUSE EMBRYONIC STEM CELLS." Reproduction, Fertility and Development 24, no. 1 (2012): 222. http://dx.doi.org/10.1071/rdv24n1ab220.

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Human and mouse embryonic stem (ES) cells are derived from the inner cell mass of preimplantation blastocysts and human ES cells were long thought to be equivalent to mouse ES cells, despite clear morphological difference and different signalling pathways to maintain their pluripotency between these two ES cell types. Mouse ES cells depend on leukemia inhibitory factor (LIF) and bone morphogenic protein 4 (BMP4) signalling, whereas their human counterparts rely on basic fibroblast growth factor (bFGF) and activin A signalling. The biggest difference of two ES cells is the ability of chimera fo

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8

Nakamura, Takayuki, Shinichi Mizuno, Hidetoshi Ozawa, et al. "A Functional Analysis of SNP on Anthracycline-Induced Cardiotoxicity in a Uniform Genetic Background Using CRISPR/Cas9 Mediated Single-Base-Edited iPSCs." Blood 134, Supplement_1 (2019): 5748. http://dx.doi.org/10.1182/blood-2019-123932.

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[Introduction] Anthracycline-induced cardiotoxicity (ACT) has been a major problem in leukemia therapy, and reported to be associated with several candidates of single nucleotide polymorphisms (SNPs). C242T polymorphism (rs4673) in the cytochrome b-245 alpha chain (CYBA) gene, which encodes superoxide-generating nicotinamide adenine dinucleotide phosphate (NADPH) oxidase light chain subunit, is focused on as one of ACT related SNPs. Several clinical studies indicated that rs4673 T allele increases the risk of ACT, however, it is unclear how rs4673 affects ACT. Here, we established a series of

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9

Chen, Hong, Bailin He, Suzhen Zhong, et al. "Disruption of SOCS1 Promotes the Antitumor Activity of CD19-Specific CAR NK Cells Coexpressing IL-15." Blood 142, Supplement 1 (2023): 6829. http://dx.doi.org/10.1182/blood-2023-182516.

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Background Chimeric antigen receptor (CAR) expressing NK cells exhibit robust antitumor responses in patients with hematologic malignancies but have limited persistent without cytokines supplement. Interleukin-15 (IL-15) promotes NK cells expansion and survival, while IL-15 signaling is often inhibited by the suppressor of cytokine signaling (SOCS) proteins (CIS, SOCS1-7), which is rapidly induced in response to IL-15 and negatively regulates cytokine signaling through the JAK/STAT pathway in NK cells. We hypothesized that SOCS1 would be a logical checkpoint to target in human NK cells to incr

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10

Ulrich, MJ, and TJ Ley. "Function of normal and mutated gamma-globin gene promoters in electroporated K562 erythroleukemia cells." Blood 75, no. 4 (1990): 990–99. http://dx.doi.org/10.1182/blood.v75.4.990.990.

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Abstract We examined the importance of cis-acting regulatory elements of the human gamma-globin gene promoter in a cell line (K562) where this gene normally functions. A gamma-Globin promoter fragments were fused to the neomycin phosphotransferase (neoR) gene in a plasmid-based vector and transiently transfected by electroporation into K562 cells. Correctly initiated “A gamma-neo” transcripts were detected with an S1 nuclease protection assay that was internally controlled for transfection efficiency and RNA content. We first optimized the conditions for electroporation, and then determined in

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11

Ulrich, MJ, and TJ Ley. "Function of normal and mutated gamma-globin gene promoters in electroporated K562 erythroleukemia cells." Blood 75, no. 4 (1990): 990–99. http://dx.doi.org/10.1182/blood.v75.4.990.bloodjournal754990.

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We examined the importance of cis-acting regulatory elements of the human gamma-globin gene promoter in a cell line (K562) where this gene normally functions. A gamma-Globin promoter fragments were fused to the neomycin phosphotransferase (neoR) gene in a plasmid-based vector and transiently transfected by electroporation into K562 cells. Correctly initiated “A gamma-neo” transcripts were detected with an S1 nuclease protection assay that was internally controlled for transfection efficiency and RNA content. We first optimized the conditions for electroporation, and then determined input DNA c

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12

Hurtado López, Ana M., Tzu Hua Chen-Liang, María Zurdo, et al. "Notch1 mutation in B Chronic Lymphocytic Lymphocytes Induces Proliferation through Cell Cycle G1/S Phase-Specific Cyclin Overexpression." Blood 134, Supplement_1 (2019): 851. http://dx.doi.org/10.1182/blood-2019-127960.

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Background and Aim: Chronic lymphocytic leukemia (CLL) is an incurable neoplasm with considerable clinical heterogeneity that is underpinned by distinct genetic changes. This poses a great challenge to the development of effective treatment, as responses to novel therapies may vary depending upon the biological subtype of disease. NOTCH1 has emerged as the most commonly mutated gene in CLL at diagnosis, its frequency rises with progression, and the variant c.7541_7542delCT accounts for approximately 80% of cases. NOTCH1-mutated (NOTCH1mut) CLL subset represents a subtype that responds poorly t

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13

Brown, Elizabeth, Ling Felce, Gillian Farnie, and Alex Bullock. "Optimisation of the delivery of ribonucleoprotein (RNP) complexes into SU-DIPG-IV cells by electroporation." August 19, 2019. https://doi.org/10.5281/zenodo.3990047.

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A report/protocol for the optimisation of delivery of ribonucleoprotein (RNP) complexes into the patient derived DIPG cell line (SU-DIPG-IV) using electroporation. Electroporation was performed with the Neon electroporation system. RNPs consisting of sgRNA and AlexaFluor488 anti-mouse antibodies were used as a proxy for the Cas9/sgRNA RNPs to be delivered for CRISPR/Cas9 mediated genome editing.

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14

Subburaj, Saminathan, and Sarah Zanon Agapito-Tenfen. "Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro−transfection." Frontiers in Plant Science 14 (September 29, 2023). http://dx.doi.org/10.3389/fpls.2023.1255819.

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The soybean (Glycine max L.) is an important crop with high agronomic value. The improvement of agronomic traits through gene editing techniques has broad application prospects in soybean. The polyethylene glycol (PEG)-mediated cell transfection has been successfully used to deliver the CRISPR/Cas9-based ribonucleoprotein (RNP) into soybean protoplasts. However, several downstream analyses or further cell regeneration protocols might be hampered by PEG contamination within the samples. Here in this study, we attempted to transfect CRISPR/Cas9 RNPs into trifoliate leaf-derived soybean protoplas

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15

Schomberg, Dominic, and Dhanansayan Shanmuganayagam. "Optimization of Schwann Cell Differentiation from Porcine Stem Cells." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.807.15.

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Swine as a translational animal model of cancer is becoming increasingly recognized for its value in closing the gap between basic research and the development of clinically effective therapies. While rodent models have helped elucidate the complexities of many mechanistic pathways of cancer, they often have physiological and genetic differences that greatly limit the translatability of results to humans. The recognition of the value of swine models has also created a need for swine‐based in vitro cell culture platforms for mechanistic studies and drug screening. Such platforms are particularl

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16

Datsomor, Alex K., Ragnhild Wilberg, Jacob S. Torgersen, Simen R. Sandve, and Thomas N. Harvey. "Efficient transfection of Atlantic salmon primary hepatocyte cells for functional assays and gene editing." G3 Genes|Genomes|Genetics, February 14, 2023. http://dx.doi.org/10.1093/g3journal/jkad039.

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Abstract The expansion of genomic resources for Atlantic salmon over the past half decade has enabled efficient interrogation of genetic traits by large-scale correlation of genotype to phenotype. Moving from correlation to causation will require genotype-phenotype relationships to be tested experimentally in a cost-efficient and cell context relevant manner. To enable such future experiments, we have developed a method for the isolation and genetic manipulation of primary hepatocytes from Atlantic salmon for use in heterologous expression, reporter assay, and gene editing experiments. We chos

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