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Journal articles on the topic 'Nested RT-PCR'

Author: Grafiati

Published: 5 June 2025

Last updated: 16 July 2025

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1

Ishchenko, L. M., V. V. Nedosekov, V. D. Ishchenko, et al. "Improving of the Nested PCR for Detection of Bovine Leukemia Virus." Mikrobiolohichnyi Zhurnal 83, no. 3 (2021): 56–65. http://dx.doi.org/10.15407/microbiolj83.03.056.

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Enzootic bovine leukosis caused by a bovine leukemia virus has a significant economic impact and is reported in World Organization for Animal Health(OIE). Aim. The purpose of our work was to improve the nested polymerase chain reaction (PCR) recommended by the OIE conducting it second-stage in real-time (RT) PCR. Such modification does not require the stage of gel electrophoresis and consequently reduces contamination risks and prevents false positive results. Methods. Primers that are recommended by the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (OIE) were used for the fi

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Lee, Si Won, Yong-Gil Shin, Jin-Young Lee, Young-Suk Kim, Mi Hee Yang, and In-Cheol Choi. "Development of a diagnostic system to detect potato virus T using RT-PCR and nested PCR." CNU Journal of Agricultural Science 42, no. 2 (2015): 99–103. http://dx.doi.org/10.7744/cnujas.2015.42.2.099.

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3

Cook, Marcia, and William H. Lynch. "A Sensitive Nested Reverse Transcriptase PCR Assay To Detect Viable Cells of the Fish Pathogen Renibacterium salmoninarum in Atlantic Salmon (Salmo salarL.)." Applied and Environmental Microbiology 65, no. 7 (1999): 3042–47. http://dx.doi.org/10.1128/aem.65.7.3042-3047.1999.

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ABSTRACT A nested reverse transcriptase (RT) PCR assay detected mRNA of the salmonid pathogen Renibacterium salmoninarum in samples of RNA extracts of between 1 and 10 cells. Total RNA was extracted from cultured bacteria, Atlantic salmon (Salmo salar L.) kidney tissue and ovarian fluid seeded with the pathogen, and kidney tissue from both experimentally challenged and commercially raised fish. Following DNase treatment, extracted RNA was amplified by both RT PCR and PCR by using primers specific for the gene encoding the major protein antigen of R. salmoninarum. A 349-bp amplicon was detected

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4

Umthun, Angela R., and William L. Mengeling. "Restriction fragment length polymorphism analysis of strains of porcine reproductive and respiratory syndrome virus by use of a nested-set reverse transcriptase-polymerase chain reaction." American Journal of Veterinary Research 60, no. 7 (1999): 802–6. http://dx.doi.org/10.2460/ajvr.1999.60.07.802.

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Abstract Objective To increase the timeliness and sensitivity of a procedure that uses viral nucleic acid amplification followed by restriction fragment length polymorphism (RFLP) analysis for identifying strains of porcine reproductive and respiratory syndrome virus (PRRSV). Sample Population 24 strains of PRRSV. Procedure A nested-set reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and compared with a nonnested-set RT-PCR for sensitivity in amplifying known quantities of infective PRRSV. Once reaction conditions were optimized, the nested-set RT-PCR was tested for effe

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Moreli, Marcos L�zaro, Victor Hugo Aquino, Ana Cec�lia R. Cruz, and Luiz Tadeu M. Figueiredo. "Diagnosis of Oropouche virus infection by RT-nested-PCR." Journal of Medical Virology 66, no. 1 (2001): 139–42. http://dx.doi.org/10.1002/jmv.2122.

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6

Cavalcante, Francisco Roger Aguiar, Alice Andrioli, Raymundo Rizaldo Pinheiro, et al. "Detecção do vírus da Artrite Encefalite Caprina por nested PCR e nested RT-PCR em ovócitos e fluido uterino." Arquivos do Instituto Biológico 80, no. 4 (2013): 381–86. http://dx.doi.org/10.1590/s1808-16572013000400002.

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A Artrite Encefalite Caprina (CAE) é uma enfermidade infectocontagiosa causada por um vírus pertencente ao gênero lentivírus, denominado de vírus da Artrite Encefalite Caprina (CAEV). O CAEV é encontrado em vários tecidos, como o nervoso, o pulmonar, o da glândula mamária e do trato genital masculino e feminino. Desta forma, objetivou-se com este trabalho identificar a presença do CAEV, pelas técnicas de diagnóstico moleculares, em ovócitos e fluido uterino, visando avaliar a possibilidade de transmissão do CAEV pela reprodução. Foram selecionadas 13 cabras comprovadamente infectadas, as quais

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PFEFFER, M., B. LINSSEN, M. D. PARKER, and R. M. KINNEY. "Specific Detection of Chikungunya Virus Using a RT-PCR/Nested PCR Combination." Journal of Veterinary Medicine Series B 49, no. 1 (2002): 49–54. http://dx.doi.org/10.1046/j.1439-0450.2002.00535.x.

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Bernal-Vera, Cynthia Magdalena, Cardozo-Segovia Fátima María, and Laura Patricia Mendoza-Torres. "Standardization of a nested RT-PCR technique for alphavirus detection." Memorias del Instituto de Investigaciones en Ciencias de la Salud 15, no. 2 (2017): 30–36. http://dx.doi.org/10.18004/mem.iics/1812-9528/2017.015(02)30-036.

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9

Green, J., K. Henshilwood, C. I. Gallimore, D. W. G. Brown, and D. N. Lees. "A Nested Reverse Transcriptase PCR Assay for Detection of Small Round-Structured Viruses in Environmentally Contaminated Molluscan Shellfish." Applied and Environmental Microbiology 64, no. 3 (1998): 858–63. http://dx.doi.org/10.1128/aem.64.3.858-863.1998.

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ABSTRACT We describe the evaluation of a nested reverse transcriptase PCR (RT-PCR) procedure for the detection of small round-structured viruses (SRSVs) in molluscan shellfish and the application of this assay for the detection of SRSVs in commercially produced shellfish and in shellfish implicated in outbreaks of gastroenteritis. The range of virus strains detected and the sensitivity of detection were evaluated by using a representative panel of 21 well-characterized SRSV strains. The nested RT-PCR detected 15 of 21 SRSVs, demonstrating that the assay detects a broad range of SRSVs including

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Maux, M., I. Bertrand, C. Gantzer, and J. Schwartzbrod. "Estimating Giardia cyst viability using RT-PCR." Water Supply 2, no. 3 (2002): 107–15. http://dx.doi.org/10.2166/ws.2002.0092.

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The aim of this work was to evaluate the use of molecular techniques for the detection of viable Giardia cysts in the environment to assess public health issues. Three target genes were selected: the heat shock protein gene, HSP70, which is expressed in response to stress; the giardin gene, which encodes a structural protein; and, alcohol dehydrogenase E (ADHE), a novel gene encoding an enzyme involved in the metabolism of energy. We tested the efficiency of five protocols for the extraction of either genomic DNA or total RNA from Giardia cysts: two of these protocols were previously cited in

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Lee, Siwon, Eun-Ha Kang, Yong-Gil Shin, and Su-Heon Lee. "Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus." Research in Plant Disease 19, no. 3 (2013): 220–25. http://dx.doi.org/10.5423/rpd.2013.19.3.220.

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Scholl, Claudia, Karina Eiwen, Stefan Frohling, Richard F. Schlenk, Hartmut Dohner, and Konstanze Dohner. "MLL/AF9 Fusion Transcript Quantification in t(9;11)-Positive AML Identify Patients with High Risk of Relapse." Blood 104, no. 11 (2004): 3015. http://dx.doi.org/10.1182/blood.v104.11.3015.3015.

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Abstract Detection of minimal residual disease (MRD) in acute myeloid leukemia (AML) with specific gene fusions is an important tool for the assessment of response to treatment and the individual risk of relapse. We evaluated the predictive value of MLL/AF9 fusion-transcript quantification using real-time RT-PCR for disease relapse and verified the results with nested RT-PCR. A t(9;11) was identified in 27 younger AML patients (16–60 years) entered into the multicenter trials AML HD93 and AML HD98-A of the AML Study Group Ulm. One hundred seventy-eight samples (bone marrow [BM], n=84; peripher

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Mourya, Devendra T., Pragya D. Yadav, Rajeev Mehla, et al. "Diagnosis of Kyasanur forest disease by nested RT-PCR, real-time RT-PCR and IgM capture ELISA." Journal of Virological Methods 186, no. 1-2 (2012): 49–54. http://dx.doi.org/10.1016/j.jviromet.2012.07.019.

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GONÇALES, Neiva S. L., Fernando F. COSTA, José VASSALLO, and Fernando L. GONÇALES JR. "Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay." Revista do Instituto de Medicina Tropical de São Paulo 42, no. 5 (2000): 263–67. http://dx.doi.org/10.1590/s0036-46652000000500005.

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Screening blood donations for anti-HCV antibodies and alanine aminotransferase (ALT) serum levels generally prevents the transmission of hepatitis C virus (HCV) by transfusion. The aim of the present study was to evaluate the efficiency of the enzyme immunoassay (EIA) screening policy in identifying potentially infectious blood donors capable to transmit hepatitis C through blood transfusion. We have used a reverse transcriptase (RT)-nested polymerase chain reaction (PCR) to investigate the presence of HCV-RNA in blood donors. The prevalence of HCV-RNA positive individuals was compared with th

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Khamsingnok, Piyamat, Witsanu Rapichai, Amonpun Rattanasrisomporn, Oumaporn Rungsuriyawiboon, Kiattawee Choowongkomon, and Jatuporn Rattanasrisomporn. "Comparison of PCR, Nested PCR, and RT-LAMP for Rapid Detection of Feline Calicivirus Infection in Clinical Samples." Animals 14, no. 16 (2024): 2432. http://dx.doi.org/10.3390/ani14162432.

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Feline calicivirus (FCV) is a highly contagious virus that causes upper respiratory tract disease, commonly known as cat flu. It is widely distributed worldwide and poses a major threat to feline health. Therefore, it is essential to find an efficient and rapid method for detecting FCV. In this study, the colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay, using neutral red as an indicator, was developed and validated to target the ORF2 gene of FCV for the first time. Additionally, the study compared the diagnostic abilities of polymerase chain reaction (

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Dandale, M. "Intravitam Diagnosis of Rabies from Saliva by Nested RT-PCR." IOSR Journal of Agriculture and Veterinary Science 1, no. 1 (2012): 08–11. http://dx.doi.org/10.9790/2380-0110811.

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Baier, Gottfried, David Telford, Erich Gulbins, Nobuo Yamada, Toshiaki Kawakami, and Amnon Altman. "Improved specificity of RT-PCR amplifications using nested cDNA primers." Nucleic Acids Research 21, no. 5 (1993): 1329–30. http://dx.doi.org/10.1093/nar/21.5.1329.

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Bertolini, Edson, Antonio Olmos, María M. López, and Mariano Cambra. "Multiplex Nested Reverse Transcription-Polymerase Chain Reaction in a Single Tube for Sensitive and Simultaneous Detection of Four RNA Viruses and Pseudomonas savastanoi pv. savastanoi in Olive Trees." Phytopathology® 93, no. 3 (2003): 286–92. http://dx.doi.org/10.1094/phyto.2003.93.3.286.

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A multiplex nested reverse transcription-polymerase chain reaction (RT-PCR) in a single closed tube was developed for the simultaneous detection of four RNA viruses: Cucumber mosaic virus, Cherry leaf roll virus, Strawberry latent ringspot virus, and Arabis mosaic virus, and the bacterium Pseudomonas savastanoi pv. savastanoi. The method enabled, for the first time, the sensitive and simultaneous detection of RNA and DNA targets from plant viruses and a bacterium, saving time, decreasing risks of contamination, and reducing costs compared with conventional monospecific nested amplifications. T

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Vikrant, Sharma, Kumar Deepak, Dhull Divya, Kaushik Sulochana, Parkash Yadav Jaya, and Kaushik Samander. "Molecular Detection of Hepatitis C Virus (HCV) by Conventional One-step RT-PCR Coupled with Nested PCR." International Blood Research & Reviews 7, no. 2 (2017): 1–6. https://doi.org/10.9734/IBRR/2017/34463.

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<strong>Aims:</strong> HCV causes both acute and chronic infections and can be easily transmitted through contaminated blood or other body fluids. The present study deals with the molecular detection of HCV with help of one-step RT-PCR assay followed by nested PCR and agarose gel electrophoresis. <strong>Study Design:</strong> RNA extracted from the confirmed positive samples of HCV was utilized for the standardization of the one-step RT-PCR assay and nested PCR assay for diagnosis of HCV. <strong>Place and Duration of Study:</strong> Centre for Biotechnology, Maharshi Dayanand University, Roh

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Chapron, Christopher D., Nicola A. Ballester, Justin H. Fontaine, Christine N. Frades, and Aaron B. Margolin. "Detection of Astroviruses, Enteroviruses, and Adenovirus Types 40 and 41 in Surface Waters Collected and Evaluated by the Information Collection Rule and an Integrated Cell Culture-Nested PCR Procedure." Applied and Environmental Microbiology 66, no. 6 (2000): 2520–25. http://dx.doi.org/10.1128/aem.66.6.2520-2525.2000.

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ABSTRACT We evaluated the use of an integrated cell culture-reverse transcription-PCR (ICC-RT-PCR) procedure coupled with nested PCR to detect human astroviruses, enteroviruses, and adenovirus types 40 and 41 in surface water samples that were collected and evaluated by using the Information Collection Rule (ICR) method. The results obtained with the ICC-RT-PCR–nested PCR method were compared to the results obtained with the total culturable virus assay–most-probable-number (TCVA-MPN) method, the method recommended by the U.S. Environmental Protection Agency for monitoring viruses in surface a

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Trujillo, Esperanza, Alejandra Collazos, and Mauricio Camargo. "PCR for equine infectious anemia virus detection in whole blood from naturally infected horses in Colombia." Actualidades Biológicas 23, no. 75 (2017): 41–46. http://dx.doi.org/10.17533/udea.acbi.329598.

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The equine infectious anemia virus is associated with a variety of clinical symptoms that include anorexia, weight loss, pirexia, and anemia. Serological tests and reverse transcriptase nested polymerase chain reaction (RT-nPCR) have been used to detect EIAV in infected horses. In the present study, the use of non-nested PCR for the detection of EIAV gag gene sequences in peripheral blood cells DNA from horses bred in farms around Medellín (Colombia) is reported. EIAV DNA was detected by PCR in blood cells from fifteen horses, which had been found positive in an agar gel immunodiffusion test,

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Wang, Lih-Chiann, Wei-En Hsu, Wei Thong, Ting-Yen Chao, and Ching-Ho Wang. "LOW SPECIFICITY OF A NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION TO DETECT AVIAN INFLUENZA VIRUS NUCLEOPROTEIN GENE." Taiwan Veterinary Journal 43, no. 02 (2016): 75–79. http://dx.doi.org/10.1142/s168264851550033x.

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Reverse transcription polymerase chain reaction (RT-PCR) was used routinely to detect the avian influenza virus (AIV) nucleoprotein (NP) gene. The purpose of the present study was to compare the correctness of a nested RT-PCR (nRT-PCR), one conventional RT-PCR with its outer primer (oRT-PCR) and the other conventional RT-PCR with its inner primer (iRT-PCR) to detect AIV NP gene. A total of 365 AI-free fecal swabs (73 pools), 7 tracheal swabs and anllantoic fluid from 25 chicken embryos were used to determine the analytic specificities of those tests. Compared with the iRT-PCR, the nRT-PCR was

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Manuel, Clyde S., Cassandra Suther, Matthew D. Moore, and Lee-Ann Jaykus. "Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization." PLOS ONE 16, no. 4 (2021): e0248581. http://dx.doi.org/10.1371/journal.pone.0248581.

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Human norovirus (NoV) is the leading cause of acute viral gastroenteritis and a major source of foodborne illness. Detection of NoV in food and environmental samples is typically performed using molecular techniques, including real-time reverse transcription polymerase chain reaction (RT-PCR) and less frequently, nested real-time PCR. In this study, we conducted a controlled comparison of two published NoV detection assays: a broadly reactive one-step real-time RT-PCR and a two-step nested real-time PCR assay. A 20% human fecal suspension containing a genogroup II human NoV was serially dilute

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Asano, Karen M., Sibele P. Souza, Sheila O. S. Silva, Leonardo J. Richtzenhain, and Paulo E. Brandão. "Rapid detection of bovine coronavirus by a semi-nested RT-PCR." Pesquisa Veterinária Brasileira 29, no. 11 (2009): 869–73. http://dx.doi.org/10.1590/s0100-736x2009001100001.

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Bovine coronavirus (BCoV) is a member of the group 2 of the Coronavirus (Nidovirales: Coronaviridae) and the causative agent of enteritis in both calves and adult bovine, as well as respiratory disease in calves. The present study aimed to develop a semi-nested RT-PCR for the detection of BCoV based on representative up-to-date sequences of the nucleocapsid gene, a conserved region of coronavirus genome. Three primers were designed, the first round with a 463bp and the second (semi-nested) with a 306bp predicted fragment. The analytical sensitivity was determined by 10-fold serial dilutions of

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Cortez, Adriana, Vanessa Souza, Telma Fernandes, and Jane Megid. "Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper." Brazilian Journal of Veterinary Research and Animal Science 52, no. 4 (2015): 363. http://dx.doi.org/10.11606/issn.1678-4456.v52i4p363-365.

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Foi comparado o valor diagnóstico das técnicas de RT-PCR e heminested RT-PCR (hnRT-PCR) em amostras de cérebro de cães com sintomatologia nervosa compatível com cinomose. Fragmentos do sistema nervoso central (SNC) colhidos de 68 animais foram testados pela Imunofluorescência direta (IFD) e, independentemente do resultado, foram armazenados a -20°C por pelo menos três anos. Após esse período, foram submetidos a RT-PCR e a hnRT-PCR com oligonucleotídeos iniciadores direcionados ao gene codificador da nucleoproteína N. As proporções de resultados positivos/examinados foram: 59/68 para a IFD, 40/

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Jang, Seong Sik, Ji Yeong Noh, Van Thi Lo, et al. "The Epidemiological Characteristics of the Korean Bat Paramyxovirus between 2016 and 2019." Microorganisms 8, no. 6 (2020): 844. http://dx.doi.org/10.3390/microorganisms8060844.

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Bats are considered reservoirs of severe emerging human pathogens. Notably, bats host major mammalian paramyxoviruses from the family Paramyxoviridae, order Mononegavirales. In this study, paramyxoviruses were investigated by reverse transcription semi-nested polymerase chain reaction (RT-semi-nested PCR) and reverse transcription polymerase chain reaction (RT-PCR), based on the RT-semi-nested PCR using the consensus paramyxovirus primers targeting the RNA dependent-RNA-polymerase (RdRp) region. In addition, RT-PCR was performed using newly designed primers targeting regions of the fusion prot

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Szemes, Marianna, Miklós Kálmán, Arben Myrta, et al. "Integrated RT–PCR/nested PCR diagnosis for differentiating between subgroups of plum pox virus." Journal of Virological Methods 92, no. 2 (2001): 165–75. http://dx.doi.org/10.1016/s0166-0934(00)00284-6.

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LAURSEN, ALEX L., JØRGEN INGERSLEV, PAUL L. ANDERSEN, and LARS ØTERGAARD. "Viremia in chronic hepatitis C patients evaluated by the Amplicor RT-PCR, a nested RT-PCR, and transaminase levels." APMIS 106, no. 1-6 (1998): 334–38. http://dx.doi.org/10.1111/j.1699-0463.1998.tb01354.x.

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Bimantara, Arif, Nosa Septiana Anindita, Taofani Rizal Al Amin, Wantonoro Wantonoro, and Heni Setianah. "Detection of Tilapia Lake Virus (TiLV) in Tilapia (Oreochromis niloticus) by Semi-Nested Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Method." International Journal of Health Science and Technology 5, no. 2 (2023): 159–65. http://dx.doi.org/10.31101/ijhst.v5i2.3316.

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Tilapia productivity is influenced by many factors, one of which is Tilapia Lake Virus (TiLV) disease, which can cause a mortality rate due to infection reaching 90%. The development of nested PCR or semi-nested PCR methods can increase the sensitivity of detection of this virus. This study aims to detect the spread of TiLV attacking tilapia early using the semi-nested PCR method with primers designed in this study. Tilapia with TiLV symptoms were taken from a tilapia farmer in Sleman Regency, Yogyakarta Special Region Province. The fish is dissected, and the kidney and lymph target organs are

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Cecílio, A. B., A. L. Cândido, M. Resende, E. D. Bontempo, and A. S. Martins. "Detection of mouse hepatitis virus in mouse colonies using the nested polymerase chain reaction." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 52, no. 4 (2000): 307–12. http://dx.doi.org/10.1590/s0102-09352000000400003.

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A reverse transcriptase polymerase chain reaction (RT-PCR) to detect mouse hepatitis virus (MHV) in hepatic tissue was developed. To circumvent possible failures in RT-PCR amplifications, a second round of PCR with internal primers was used to confirm the specificity and increase the sensitivity of the test. Using this method specific amplification of MHV sequences was observed in 18 out of 20 mouse colonies examined.

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Varveri, C. "DIRECT NESTED RT-PCR FOR THE DETECTION OF PLUM POX VIRUS." Acta Horticulturae, no. 657 (September 2004): 155–58. http://dx.doi.org/10.17660/actahortic.2004.657.20.

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Ré, Viviana, Lorena Spinsanti, Adrián Farías, et al. "Reliable detection of St. Louis encephalitis virus by RT-nested PCR." Enfermedades Infecciosas y Microbiología Clínica 26, no. 1 (2008): 10–15. http://dx.doi.org/10.1157/13114389.

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Happ, George M., Elizabeth Aquilla, Monika Martick, Christine Yuncker, Janet Wojciechowski, and Leslie Fox. "DLA-DRB1 histocompatibility genotyping using RT-nested PCR and cycle sequencing." Veterinary Immunology and Immunopathology 69, no. 2-4 (1999): 93–100. http://dx.doi.org/10.1016/s0165-2427(99)00045-8.

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Salem, Abid Nabil Ben, A. Chupin Sergei, P. Bjadovskaya Olga, G. Andreeva Olga, Aouni Mahjoub, and B. Prokhvatilova Larissa. "Multiplex nested RT-PCR for the detection of porcine enteric viruses." Journal of Virological Methods 165, no. 2 (2010): 283–93. http://dx.doi.org/10.1016/j.jviromet.2010.02.010.

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Watzka, M., A. Waha, A. Koch, et al. "An Optimized Protocol for mRNA Quantification Using Nested Competitive RT-PCR." Biochemical and Biophysical Research Communications 231, no. 3 (1997): 813–17. http://dx.doi.org/10.1006/bbrc.1997.6175.

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Loric, S., F. Dumas, P. Eschwege, et al. "Enhanced detection of hematogenous circulating prostatic cells in patients with prostate adenocarcinoma by using nested reverse transcription polymerase chain reaction assay based on prostate-specific membrane antigen." Clinical Chemistry 41, no. 12 (1995): 1698–704. http://dx.doi.org/10.1093/clinchem/41.12.1698.

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Abstract We report the development of a new sensitive nested reverse transcription-polymerase chain reaction (RT-PCR) assay, using primers derived from the prostate-specific membrane antigen (PSM) cDNA sequence, to detect an hematogenous spread of prostate adenocarcinoma cells. In 60 patients with a biopsy-proven prostate cancer, PSM and PSA RT-PCR detected circulating prostate cells in 40 and 20 patients, respectively. In pT4 M+ and pT3 M+ disease patients, nested PSM primers detected cells in 28 of 33 patients (85%), whereas nested PSA primers detected cells in 17 of 33 (51%). In patients wi

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Menschikowski, Mario, Margot Vogel, Rolf Eckey, Gerd Dinnebier, and Werner Jaross. "In SituReverse Transcriptase-Nested Polymerase Chain Reaction to Identify Intracellular Nucleic Acids without the Necessity of Dnase Pretreatment and Hybridisation." Analytical Cellular Pathology 22, no. 3 (2001): 151–58. http://dx.doi.org/10.1155/2001/654016.

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In the present study a protocol of in situ reverse transcriptase‐nested polymerase chain reaction (in situ RT‐nested PCR) was examined based on the following modifications. (i) To exclude false positive signals caused by “DNA repair mechanisms” and “endogenous priming”, a two‐step PCR was applied after reverse transcription. The first step was performed in the presence of extrinsic primers and unlabeled nucleotides with a maximum of PCR cycles possible without destroying the cell morphology. The second step consisted of only one annealing/elongation reaction, the target sequence marked by addi

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Andeweg, Arno C., Theo M. Bestebroer, Martijn Huybreghs, Tjeerd G. Kimman, and Jan C. de Jong. "Improved Detection of Rhinoviruses in Clinical Samples by Using a Newly Developed Nested Reverse Transcription-PCR Assay." Journal of Clinical Microbiology 37, no. 3 (1999): 524–30. http://dx.doi.org/10.1128/jcm.37.3.524-530.1999.

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This paper describes the development and evaluation of a new nested reverse transcription (RT)-PCR for the detection of rhinovirus in clinical samples. The nucleotide sequences of the 5′ noncoding regions of 39 rhinoviruses were determined in order to map the most conserved subregions. We designed a set of rhinovirus-specific primers and probes directed to these subregions and developed a new nested RT-PCR. The new assay includes an optimal RNA extraction method and amplicon identification with probe hybridization to discriminate between rhinoviruses and the closely related enteroviruses. It p

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Weidmann, M., P. Schmidt, M. Vackova, K. Krivanec, P. Munclinger, and F. T. Hufert. "Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT)-PCR and TaqMan RT-PCR." Journal of Clinical Microbiology 43, no. 2 (2005): 808–12. http://dx.doi.org/10.1128/jcm.43.2.808-812.2005.

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Kim, Yong Hwan, Kyu Woan Cho, Hwa Young Youn, Han Sang Yoo, and Hong Ryul Han. "Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR." Journal of Veterinary Science 2, no. 1 (2001): 59. http://dx.doi.org/10.4142/jvs.2001.2.1.59.

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Koutná, M., T. Vesely, I. Psikal, and J. Hulová. "Identification of spring viraemia of carp virus (SVCV) by combined RT-PCR and nested PCR." Diseases of Aquatic Organisms 55 (2003): 229–35. http://dx.doi.org/10.3354/dao055229.

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Halabi, Susan, Eric J. Small, Daniel F. Hayes, Nicholas J. Vogelzang, and Philip W. Kantoff. "Prognostic Significance of Reverse Transcriptase Polymerase Chain Reaction for Prostate-Specific Antigen in Metastatic Prostate Cancer: A Nested Study Within CALGB 9583." Journal of Clinical Oncology 21, no. 3 (2003): 490–95. http://dx.doi.org/10.1200/jco.2003.04.104.

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Purpose: To determine whether reverse transcriptase polymerase chain reaction (RT-PCR) to detect circulating prostate-specific antigen (PSA)-positive cells is a prognostic factor for survival in hormone refractory prostate cancer and to validate the prognostic importance of this test in relation to other known prognostic factors. Patients and Methods: A single centralized laboratory received and analyzed whole blood for RT-PCR for PSA for a subset of patients enrolled on two Cancer and Leukemia Group B (CALGB) randomized trials (CALGB 9583 and CALGB 9480). Using 9583, a prognostic model was de

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Matsuda, Yoshinori, Takeshi Sameshima, Nobuyuki Moriura, et al. "Identification of Individual Powdery Mildew Fungi Infecting Leaves and Direct Detection of Gene Expression by Single Conidium Polymerase Chain Reaction." Phytopathology® 95, no. 10 (2005): 1137–43. http://dx.doi.org/10.1094/phyto-95-1137.

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Greenhouse-grown tomato seedlings were inoculated naturally with two genera of powdery mildew conidia forming appressorial germ tubes that could not be differentiated by length alone. For direct identification, single germinated conidia were removed from leaves by means of a glass pipette linked to the manipulator of a high-fidelity digital microscope. This microscope enabled in vivo observation of the fungi without leaf decoloration or fungal staining. The isolated conidia were subjected to PCR amplification of the 5.8S rDNA and its adjacent internal transcribed spacer sequences followed by n

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Lin, Johnson, and Atheesha Singh. "Detection of human enteric viruses in Umgeni River, Durban, South Africa." Journal of Water and Health 13, no. 4 (2015): 1098–112. http://dx.doi.org/10.2166/wh.2015.238.

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The prevalence of adenovirus (AdV), rotaviruses (RV) and enteroviruses (EV) in Umgeni River waters of Durban, South Africa was assessed qualitatively and quantitatively during April 2011 to January 2012 using polymerase chain reaction (PCR)/reverse transcription-polymerase chain reaction (RT-PCR), nested PCR and quantitative PCR (qPCR), as well as nested integrated cell culture PCR (nested ICC-PCR). The phylogenetic analysis of the adenovirus and enterovirus amplicons was also performed. The nested PCR results effectively detected the presence of AdV and EV in all water samples. The results of

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Souza, Sibele Pinheiro de, Karen Miyuki Asano, Laura Yaneth Villarreal Buitrago, Sheila De Oliveira Souza Silva, Leonardo José Richtzenhain, and Paulo Eduardo Brandão. "Reação de hemi-nested RT-PCR dirigida ao gene da hemaglutinina-esterase do Coronavírus Bovino." Brazilian Journal of Veterinary Research and Animal Science 47, no. 4 (2010): 323. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2010.26832.

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O coronavírus bovino (BCoV) é um vírus RNA simples fita, de sentido positivo, não segmentado com envelope constituído de uma camada dupla de lipídios com quatro proteínas (HE, S, E e M) que resultam no aspecto de coroa dos vírions. Como a HE (hemaglutinina-esterase) é uma proteína polimórfica com uma função de receptor aglutinante secundária, estudos sobre a diversidade do gene HE podem possibilitar maiores informações sobre a evolução e interação hospedeiro-parasita do BCoV. Uma reação de hemi-nested RT-PCR foi desenvolvida para a amplificação de um produto de 441pb do gene HE do BCoV (nt 543

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NIITSUMA, Hirofumi, Motoyasu ISHII, Tomoo KOBAYASHI, Chiaki SUZUKI, Koju KOBAYASHI, and Takayoshi OYOTA. "Development of RT-nested PCR for specific detection of hepatitis G virus." Kanzo 37, no. 11 (1996): 620–25. http://dx.doi.org/10.2957/kanzo.37.620.

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Moreli, Marcos Lázaro, Victor Hugo Aquino, and Luiz Tadeu M. Figueiredo. "Identification of Simbu, California and Bunyamwera serogroup bunyaviruses by nested RT-PCR." Transactions of the Royal Society of Tropical Medicine and Hygiene 95, no. 1 (2001): 108–13. http://dx.doi.org/10.1016/s0035-9203(01)90354-2.

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Wu, Qingfa, Zuyuan Xu, Tian Wei, et al. "Development of Taqman RT-nested PCR system for clinical SARS-CoV detection." Journal of Virological Methods 119, no. 1 (2004): 17–23. http://dx.doi.org/10.1016/j.jviromet.2004.02.011.

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Seilner, L. N., R. J. Coelen, and J. S. Mackenzie. "Sensitive detection of Ross River virus — a one-tube nested RT-PCR." Journal of Virological Methods 49, no. 1 (1994): 47–58. http://dx.doi.org/10.1016/0166-0934(94)90054-x.

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Bansal, K. "Sensitivity Comparison of Nested RT-PCR with Immunofluorescence for Diagnosis of Rabies." IOSR Journal of Pharmacy and Biological Sciences 2, no. 6 (2012): 9–11. http://dx.doi.org/10.9790/3008-0260911.

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