Academic literature on the topic 'Neutrophils and Red hepatization'
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Journal articles on the topic "Neutrophils and Red hepatization"
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Adedeji, S. O., E. O. Ogunba, and O. O. Dipeolu. "Synergistic effect of migrating Ascaris larvae and Escherichia coli in piglets." Journal of Helminthology 63, no. 1 (1989): 19–24. http://dx.doi.org/10.1017/s0022149x00008671.
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ABSTRACTThe effect of intestinal flora on the establishment, development and pathogenicity of Ascaris suum larvae in piglets (Large White breed) was investigated. The infected piglets with Ascaris and Escherichia coli showed signs of pneumonia, cough with respiratory difficulties initially even though these moderated with time. They lost appetite and showed signs of unthriftiness with loss of weight. The packed cell volume was normal but the differential leucocyte counts of the pigs infected with Ascaris larvae and bacteria had high neutrophils, unlike the very high lymphocyte count observed in piglets with ascarids only. The piglets had generalized serous atrophy of body fat. The pericardial and perirenal fats were gelatinous. There was a firm and nodular grey and red hepatization with abscess pockets in the intermediate and anterior one third of the diaphragmatic lobes of the lungs. The liver contained greyish-white and depressed focus immediately dorsal to the area of attachment to the gall bladder with multifocal areas. There was no significant gross lesion in the control animals. Cultural and microscopic examinations of some internal organs of the infected animals showed that bacteria were carried to the lungs by the migrating Ascaris larvae. The combined synergistic effect of Ascaris larvae and E. coli was also investigated and it was concluded that the two agents (A. suum larvae and E. coli) worked together synergistically.
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Muralinath, E., D.V. Ramanjaneyulu, Pragna K. Sravani, et al. "An Essential Parameters of Pathology of Pneumonia Include Etiology, Epidemiology, Patho Physiology, Histo Pathology, Diagnosis, Differential Diagnosis and Treatment." Journal of Advanced Research and Reviews in Virology & Microbiology 2, no. 1 (2025): 40–48. https://doi.org/10.5281/zenodo.15486599.
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<em>Pneumonia is the general name for a collection of illnesses caused by different microorganisms that result in lung parenchymal infection. Treatment recommendations for effective therapy in both inpatient and outpatient settings have been developed with the use of categorization schemata, which have also helped identify the common pathogens specifically responsible for each kind of pneumonia. Enhance interprofessional team tactics to promote communication and care coordination in order to improve outcomes for patients affected by pneumonia.</em>
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Biswas, Deblina, Anshu Kumari, George C. K. Chen, et al. "Quantitative Differentiation of Pneumonia from Normal Lungs: Diagnostic Assessment Using Photoacoustic Spectral Response." Applied Spectroscopy 71, no. 11 (2017): 2532–37. http://dx.doi.org/10.1177/0003702817708320.
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Pneumonia is an acute lung infection that takes life of many young children in developing countries. Early stage (red hepatization) detection of pneumonia would be pragmatic to control mortality rate. Detection of this disease at early stages demands the knowledge of pathology, making it difficult to screen noninvasively. We propose photoacoustic spectral response (PASR), a noninvasive elasticity-dependent technique for early stage pneumonia detection. We report the quantitative red hepatization detection of pneumonia through median frequency, spectral energy, and variance. Significant contrast in spectral parameters due to change in sample elasticity is found. The tissue-mimicking phantom study illustrates a 39% increase in median frequency for 1.5 times the change in density. On applying to formalin-fixed pneumonia-affected goat lungs, it provides a distinct change in spectral parameters between pneumonia affected areas and normal lungs. The obtained PASR results were found to be highly correlating to standard histopathology. The proposed technique therefore has potential to be a regular diagnostic tool for early pneumonia detection.
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Aoshiba, Kazutetsu, Yuri Nakajima, Shuji Yasui, Jun Tamaoki, and Atsushi Nagai. "Red Blood Cells Inhibit Apoptosis of Human Neutrophils." Blood 93, no. 11 (1999): 4006–10. http://dx.doi.org/10.1182/blood.v93.11.4006.411k18_4006_4010.
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Oxidative stress has been implicated in the triggering of apoptosis in neutrophils. Because red blood cells (RBCs) are well known to scavenge oxidants including H2O2, we tested the hypothesis that RBCs inhibit apoptosis of neutrophils by reducing intracellular oxidative stress. Apoptosis of neutrophils was evaluated by light microscopy and DNA gel electrophoresis. We found that coculture with RBCs protected against neutrophil apoptosis. Neither physical contact between RBCs and neutrophils nor the cellular integrity of RBCs was required to protect against neutrophil apoptosis. Neutrophil apoptosis was promoted by exogenous H2O2 but suppressed by catalase, indicating a role for H2O2 as a mediator of apoptosis. The protective effect of RBCs against apoptosis was due to catalase and glutathione metabolism because blocking of these antioxidant systems in RBCs attenuated the protective effect of RBCs. These results suggest that neutrophils are protected against apoptosis in the circulation.
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Aoshiba, Kazutetsu, Yuri Nakajima, Shuji Yasui, Jun Tamaoki, and Atsushi Nagai. "Red Blood Cells Inhibit Apoptosis of Human Neutrophils." Blood 93, no. 11 (1999): 4006–10. http://dx.doi.org/10.1182/blood.v93.11.4006.
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Abstract Oxidative stress has been implicated in the triggering of apoptosis in neutrophils. Because red blood cells (RBCs) are well known to scavenge oxidants including H2O2, we tested the hypothesis that RBCs inhibit apoptosis of neutrophils by reducing intracellular oxidative stress. Apoptosis of neutrophils was evaluated by light microscopy and DNA gel electrophoresis. We found that coculture with RBCs protected against neutrophil apoptosis. Neither physical contact between RBCs and neutrophils nor the cellular integrity of RBCs was required to protect against neutrophil apoptosis. Neutrophil apoptosis was promoted by exogenous H2O2 but suppressed by catalase, indicating a role for H2O2 as a mediator of apoptosis. The protective effect of RBCs against apoptosis was due to catalase and glutathione metabolism because blocking of these antioxidant systems in RBCs attenuated the protective effect of RBCs. These results suggest that neutrophils are protected against apoptosis in the circulation.
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Chin‐Yee, Keeney, Krueger, Dietz, and Moses. "Supernatant from stored red cells activates neutrophils." Transfusion Medicine 8, no. 1 (1998): 49–56. http://dx.doi.org/10.1046/j.1365-3148.1998.00125.x.
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Lautenschlager, Sueli de Oliveira Silva, Tehyung Kim, Danielle Lazarim Bidóia, Celso Vataru Nakamura, Hans-Joachim Anders, and Stefanie Steiger. "Plasma Proteins and Platelets Modulate Neutrophil Clearance of Malaria-Related Hemozoin Crystals." Cells 9, no. 1 (2019): 93. http://dx.doi.org/10.3390/cells9010093.
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Hemozoin is an insoluble crystalline pigment produced by the malaria parasite Plasmodia upon digesting host hemoglobin inside red blood cells. Red blood cell rupture releases hemozoin crystals into the circulation from where they are cleared by phagocytes such as neutrophils. We speculated that plasma proteins would affect the ability of neutrophils to clear hemozoin crystals. To test this, we cultured human blood neutrophils with hemozoin ex vivo and found that neutrophils ingested hemozoin (0.1–1 µm crystal size) in a dose-dependent manner into phagosomes and vesicles/vacuoles, resulting in morphological changes including nuclear enlargement, and vesicle formation, but not cell membrane rupture or release of neutrophil extracellular traps. The presence of human plasma significantly inhibited the ability of neutrophils to ingest hemozoin crystals. Platelet-poor plasma further inhibited the uptake of hemozoin by neutrophils. Selective exposure to fibrinogen completely replicated the plasma effect. Taken together, neutrophils cleared hemozoin crystals from the extracellular space via endocytosis into phagosomes and vesicles without inducing the release of neutrophil extracellular traps. However, human plasma components such as fibrinogen limited hemozoin clearance, whereas the presence of platelets augmented this process. These factors may influence the pro-inflammatory potential of hemozoin crystals in malaria.
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Ray, Debadrita, and Narender Kumar. "Red Blood Cells Garlanding Neutrophils: An Intriguing Observation." Journal of Postgraduate Medicine, Education and Research 59, no. 1 (2025): 39–40. https://doi.org/10.5005/jp-journals-10028-1677.
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Rhyan, J. C., and D. A. Saari. "A Comparative Study of the Histopathologic Features of Bovine Tuberculosis in Cattle, Fallow Deer (Dama dama), Sika Deer (Cervus nippon), and Red Deer and Elk (Cervus elaphus)." Veterinary Pathology 32, no. 3 (1995): 215–20. http://dx.doi.org/10.1177/030098589503200301.
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Sections of tuberculous lesions from 23 elk ( Cervus elaphus nelsoni) and red deer ( Cervus elaphus elaphus), 12 fallow deer ( Dama dama), 10 sika deer ( Cervus nippon), and 30 cattle were examined and compared. Lesions were scored for caseous necrosis, mineralization, neutrophils, macrophages, giant cells, and acid-fast bacilli. Some differences in lesion morphology between the species were noted. Elk/red deer lesions had marked variation and often differed from bovine lesions in several characteristics; elk/red deer lesions usually had scattered peripheral mineralization rather than central mineralization and contained more neutrophils and fewer giant cells than did bovine lesions. Fallow deer lesions contained more giant cells but were otherwise indistinguishable from elk lesions. Sika deer lesions had more giant cells and fewer neutrophils than did lesions from cattle or other cervid species. Sika deer giant cells were larger and contained more nuclei than did giant cells in the other species.
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Franco-Penteado, Carla Fernanda, Marcos André Cavalcanti Bezerra, Mariana R. B. Mello та ін. "Increased Adhesive Properties and Reactive Oxygen Species of Red Cells, Platelets and Leukocytes and Neutrophil Chemotaxis in IVS-1-6 (T → C) Homozygous β-Thalassemia Intermedia." Blood 112, № 11 (2008): 1873. http://dx.doi.org/10.1182/blood.v112.11.1873.1873.
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Abstract The pathophysiology of b-thalassemia intermedia (TI) is characterized by extravascular hemolysis, with the release into the peripheral circulation of damaged red blood cells and erythroid precursors. In these patients vascular complications and endothelial cell activation are more frequent and activation of vascular endothelium is considered an important aspect of inflammation, vasculitis and thrombosis in this disease. The mutation at position +6 of the IVS-1 5’ (donor) consensus sequence is particularly mild and is usually associated with TI owing to a relatively high level of normal mRNA splicing. Some authors have been suggesting that red blood cells (RBC) adhesion and reactive oxygen species (ROS) are significantly increased in b-thalassemia major, however few studies evaluated these alterations in TI. The aim of this study was to evaluate expression of adhesion molecules, adhesion proprieties of platelets, RBC and neutrophils, RBC and leukocytes ROS production and neutrophil chemotaxis in TI IVS-1–6 (T → C) homozygous. Neutrophils, RBC and platelets were isolated from the peripheral blood of healthy controls (n=16) and patients with TI (n=16). Basal adhesion was compared using static adhesion assays and surface protein expression using flow cytometry. Chemotaxis of control and TI neutrophils (4x106 cells/mL in RMPI medium) were assessed using static adhesion assays and a 96-well chemotaxis chamber assay (ChemoTX, Neuroprobe). For ROS measurement, the isolated cells were incubated with 2’-7’-dichlorofluorescin diacetate (DCF) and analyzed by flow cytometry. TI neutrophils demonstrate a greater ability to adhere to fibronectin (FN)-coated plates than control individual (13.2±1.2 % neutrophils compared to 7.7±0.7 %; P=0.003 Mann-Whitney test). Similar results were found with TI red cell adhesion to FN and TI platelet adhesion to fibrinogen compared to respective cells in control individual (7.24±0.6%, 21.2±3.3% RBC and platelets adhered compared to 3.97±0.5% and 9.5 ± 1.0%, p=0.001; p= 0.005, respectively). The integrin α-subunits (CD11a and CD11b) surface expression did not differ on neutrophils from TI patients and control individual. However, the VLA-4 integrin subunit CD49d surface expression was higher on TI neutrophils compared to controls individuals (4.94±0.71% vs 2.31±0.46%; p=0.013). The surface expressions of CD36, CD49d and CD71 on the RBC of TI patients were significantly higher than on these cells in control individuals (10.93±1.83%, 1.44±0.30%, 14.3±1.83%; 2.31±0.46%; 0.63±0.10% vs 0.13±0.02% and 1.95±0.37%, p&lt;0.0001, respectively). Chemotaxis assays showed that TI neutrophils demonstrate greater spontaneous migration when compared with control neutrophils (4.1±0.29x105/mL and 2.46±0.3x105/mL; p=0.012, respectively). ROS production was significantly increased in TI RBC, neutrophils and mononuclear cells compared to the same cells in control individuals (236.2±22.8, 2352±222.8, 1255.9±193.5 vs 53.7±5.3; 1459.8±143.8, 329.6±47.6 MFC, p&lt;0.0001; p=0.010; p&lt;0.0001, respectively). In addition, superoxide dismutase (SOD) plasma activity was significantly reduced in TI compared to control plasma (4.8±1.7 vs 12.4±0.8 P=0.003, respectively). Our results show that the adherence to extracellular matrix protein was markedly enhanced for red blood cells, neutrophils and platelets from TI. Leukocytes and red blood cells of TI patients produce higher baseline levels of free radicals and the anti-oxidant mechanism in plasma is diminished. These data suggest that enhanced adherence (neutrohils, RBC and platelets), neutrophil chemotaxis and ROS production (neutrophils, mononuclear cells and RBC) may contribute to several clinical complications of β-thalassemia intermedia, such as pulmonary hypertension.
Dissertations / Theses on the topic "Neutrophils and Red hepatization"
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Fredriksson, Karin. "The role of red blood cells in inflammation and remodeling /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-040-0/.
Full textBooks on the topic "Neutrophils and Red hepatization"
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Benoit, Elna. Understanding the complete blood count: The red blood cells : red blood cells, white blood cells or platelets. Concept Media, 2006.
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Benoit, Elna. Understanding the complete blood count: The white blood cells : red blood cells, white blood cells or platelets. Concept Media, 2006.
Find full textBook chapters on the topic "Neutrophils and Red hepatization"
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Zelter, Tamir, Zvi Granot, and Ron Dzikowski. "Assaying Interactions Between Neutrophils and Plasmodium falciparum-Infected Red Blood Cells." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2189-9_47.
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Aiuti, Alessandro, Serena Scala, and Christian Chabannon. "Biological Properties of Hematopoietic Stem Cells: Scientific Basis for Hematopoietic Cell Transplantation." In The EBMT Handbook. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-44080-9_7.
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AbstractHematopoiesis—from the Greek term for “blood making”—is the adaptive process by which mature and functional blood cells are continuously replaced over the entire lifetime of an individual. Erythrocytes, platelets, and the various subsets of leukocytes all have finite although different life spans. As a consequence, the daily production of red blood cells, platelets, and neutrophils under homeostatic conditions amounts to more than 300 billion cells.
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Daniels, G. "Blood groups on red cells, platelets and neutrophils." In Blood and Bone Marrow Pathology. Elsevier, 2011. http://dx.doi.org/10.1016/b978-0-7020-3147-2.00037-7.
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Knight, Gavin. "Blood cell genesis: red cell, white cell and platelet families." In Cell Structure and Function. Oxford University Press, 2014. http://dx.doi.org/10.1093/hesc/9780199652471.003.0004.
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This chapter evaluates the types of blood cell found within peripheral blood. It begins by explaining blood cell production and the structure of the bone marrow. The red colouration of our blood is derived from the red blood cells, also called erythrocytes, and in particular the intracellular respiratory pigment haemoglobin. Meanwhile, our capacity to fight infection comes from heterogeneous populations of white blood cells, also called leucocytes, with each population having a different function. Under the umbrella term of white blood cells are three types of cell characterized by the types of granule within their cytoplasm. These cells are broadly called granulocytes, and more specifically are neutrophils, eosinophils, and basophils. The chapter then looks at stem cells, haemopoiesis, erythropoiesis, thrombopoiesis, haemostasis, granulopoiesis, and monopoiesis.
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Wallace, Daniel J. "The Body’s Protection Plan." In The Lupus Book, 7th ed. Oxford University PressNew York, 2025. http://dx.doi.org/10.1093/oso/9780197689837.003.0005.
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Abstract Whole blood consists of red blood cells, white blood cells, platelets, and plasma. There are five types of white blood cells. These include the neutrophils, which are important in acute inflammation; lymphocytes, which help regulate chronic inflammatory processes; and monocytes, which are responsible for helping the body recognize foreign material. All these cells are derived from the bone marrow. In a normal adaptive and innate immune response, these elements all work together. Lymphocytes migrate to the thymus, where they are ultimately recognized as T cells or B cells. The T cells read antigenic signals present in monocytes or macrophages and are thus able to promote or turn off inflammation and the killing of specific cells. Some B cells transform themselves into plasma cells that make immunoglobulin, which then circulates in the plasma. Immunoglobulins G, A, M, D, and E also help to destroy foreign materials.
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Brierley, Charlotte K., and David P. Steensma. "Myelodysplastic syndromes." In Oxford Textbook of Medicine, edited by Chris Hatton and Deborah Hay. Oxford University Press, 2020. http://dx.doi.org/10.1093/med/9780198746690.003.0514.
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The myelodysplastic syndromes (MDS) are marrow failure syndromes characterized by cytopenias, blood cell dysmorphology, acquired clonal cytogenetic and molecular genetic mutations, and a risk of development of acute myeloid leukaemia. MDS may evolve in patients previously treated with cytotoxic chemotherapy or radiotherapy for a solid tumour, but most commonly arise de novo in patients over 60 years old. Most patients present with features of chronic anaemia or manifestations related to thrombocytopenia or infection. The diagnosis may be suggested by the presence of normocytic or macrocytic anaemia, with the peripheral blood smear showing dysplastic changes in red blood cells or neutrophils. Bone marrow aspirate and biopsy permits detailed cytogenetic study, which is critical for diagnostic classification and prognosis. Increasingly, molecular genetic assays (next-generation sequencing panels) aid in diagnosis and prognosis. The World Health Organization (WHO) classification recognizes various MDS subtypes based on the morphological appearance of the peripheral blood and bone marrow. These include MDS with excess blasts, MDS with deletion of the long arm of chromosome 5, and MDS with ring sideroblasts. Treatment is symptomatic in most cases. The only potentially curative treatment is allogeneic bone marrow transplantation, which is often precluded due to patients’ advanced age or comorbidity. Higher-risk patients may experience a survival benefit from treatment with the DNA hypomethylating agent azacitidine, and decitabine delays disease progression to acute myeloid leukaemia. Some patients with lower-risk disease may show a response to immunosuppression with antithymocyte globulin and ciclosporin. Patients with isolated chromosome 5q deletions and lower-risk disease may respond dramatically to lenalidomide, an immunomodulatory drug.
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Dolphin, Heather, and Fatima Ahmad. "Bacteriology Diagnostic Methods." In Tutorial Topics in Infection for the Combined Infection Training Programme. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198801740.003.0015.
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This is summarized in Table 8.1. a) Microscopy— A cell count is performed on sterile fluids and CSF samples using the Neubauer chamber or a similar device. The number of white blood cells (WBC) and red blood cells seen under the microscope are reported as well as the differential WBC count (i.e. the number or percentage of lymphocytes and neutrophils in the sample). A Gram stain is then done and the presence of any organism reported. b) Culture samples are plated onto the appropriate media and streaked out for single colonies as shown. Blood agar is normally used; however, other media are used depending on the site of the specimen, e.g. chocolate agar is used if a fastidious organism is a potential pathogen such as Haemophilus sp.; anaerobic agar for anaerobes; selective agar such as MacConkey can be used on non-sterile specimens to differentiate between the colony types. Plates are incubated for eighteen to forty-eight hours at the correct conditions; most plates being CO2, others at O2 and anaerobically. c) Identification plates are examined for growth. Potentially significant isolates are identified either by MALDI-TOF MS, by API, or other biochemical tests. d) Sensitivities are performed on significant organisms by manual and automated methods. This is summarized in Table 8.2. Selective agar is necessary when isolating pathogens from faeces, although further confirmatory tests are needed. ● Black or colourless colonies on xylose lysine deoxycholate (XLD) or other chromogenic agar plates are tested with oxidase reagent. ● Oxidase negative isolates are identified by MALDI-TOF, API and or biochemically using triple-sugar iron (TSI) tubes. ● Serology is then performed on suspicious isolates and sent to a reference laboratory for confirmation. ● Campylobacter is confirmed by testing grey flat colonies on campylobacter agar with oxidase reagent. Oxidase positive samples are Gram stained and if ‘seagull’-shaped gram-negative bacteria are observed under the microscope, campylobacter is confirmed. The catalase test is a simple biochemical test to differentiate between Staphylococcus species and Streptococcus species, with the use of hydrogen peroxide (H2O2). It tests for the presence of the enzyme catalase which is found in Staphylococcus species.
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Kumar, Neeraj. "Progressive Imbalance and Visual Impairment in a Patient With Diabetes." In Mayo Clinic Cases in Neuroimmunology, edited by Andrew McKeon, B. Mark Keegan, and W. Oliver Tobin. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780197583425.003.0081.
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A 72-year-old man with hypothyroidism and type 2 diabetes sought care for a 3-year history of slowly progressive, ascending lower limb paresthesias and imbalance. Three months earlier, he noted subacute onset of finger numbness and substantial worsening of imbalance with infrequent falls. He also had a 1-year history of progressive visual decline that persisted despite cataract surgery. Additional symptoms included intermittent light-headedness and confusion. Laboratory evaluations showed a decreased hemoglobin value and an increased mean corpuscular volume. Macrocytic red blood cells were noted on a peripheral blood smear. Serum vitamin B<sub>12</sub> level was less than 70 ng/L. Levels of plasma homocysteine and serum methylmalonic acid were markedly increased to 375 µmol/L and 143 nmol/L, respectively. Serum copper level was normal. Serum parietal cell antibodies were increased to 46 U, and intrinsic factor antibodies were absent. Serum gastrin was markedly increased. The clinical presentation in this patient suggested a myeloneuropathy. His vitamin B<sub>12</sub> level was undetectable and accompanied by a macrocytic anemia and increased methylmalonic acid and homocysteine levels. Even though intrinsic factor antibodies were negative, the clinical picture was supportive of subacute combined degeneration in the setting of pernicious anemia. The patient was started on vitamin B<sub>12</sub> replacement. At 6-month follow-up he had striking improvement in gait and vision. The light-headedness and confusion were no longer present. His examination was remarkable only for mild impairment, with tandem gait and a slightly positive Romberg sign. The lower limb reflexes were reduced. Impaired position perception at the toes persisted, but vibration perception in the lower limbs improved. Laboratory investigations showed normalization of the hemoglobin, vitamin B<sub>12</sub>, methylmalonic acid, and homocysteine levels. The serum gastrin level had improved but was still increased at 742 pg/mL. The best-characterized neurologic manifestations of vitamin B<sub>12</sub> deficiency include myelopathy and myeloneuropathy. Autonomic neuropathy, optic neuropathy, and neuropsychiatric manifestations have also been reported. Neurologic manifestations may occur without evidence of the characteristic hematologic derangement, megaloblastic anemia. Macrocytosis or hypersegmented neutrophils on peripheral blood smear may be clues.
Conference papers on the topic "Neutrophils and Red hepatization"
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Schumacher, Kristopher, Aleksander Popel, Bahman Anvari, William Brownell, and Alexander Spector. "Computational Analysis of the Tether Pulling Experiment to Probe Cellular Membranes." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206324.
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Membrane tethers can form naturally such as during leukocyte rolling (pre-adhesion) stage. Tethers can also be experimentally pulled from cellular membranes to estimate membrane mechanical and electromechanical properties, including bending modulus, adhesion energy of the membrane-cytoskeleton interaction, tension, and electromechanical forces produced by membranes. This technique has been effectively applied to a variety of cells such as red blood cells [1], neutrophils [2], and cochlear outer hair cells [3,4]. Fig. 1 presents the experiment where tethers are pulled from cellular membranes using optically trapped beads.
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Cincović, Marko, Jože Starič, Jožica Ježek, et al. "Complete blood count in cows in the peripartal period and the relationship to the indicators of metabolic stress." In Zbornik radova 26. medunarodni kongres Mediteranske federacije za zdravlje i produkciju preživara - FeMeSPRum. Poljoprivredni fakultet Novi Sad, 2024. http://dx.doi.org/10.5937/femesprumns24006c.
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Lactation and metabolic rearrangement significantly influence hematological parameters. The red bloodline of early lactation cows is characterized by hypochromic changes in erythrocytes and a reduced number of erythrocytes. The reduced concentration of hemoglobin is linked to milk production, since a higher hemoglobin concentration was found in heifers (which are not naturally lactating). The number of neutrophils was the highest in the first week after parturition, while the number of lymphocytes was the lowest in the same period, so the neutrophil:lymphocyte index was the highest in this period. The number of eosinophils increased slightly during the experimental period, while the number of monocytes was the highest in the first week and then decreased. Such changes in the differential white line occur as a consequence of the action of acute stress and a sudden jump in the concentration of cortisol, which has shown its effect on immune cells. In white cattle, NEFA as an indicator of metabolic stress leads to a drop in the total number of leukocytes, and cows classified according to the NEFA and cortisol criteria show an increased percentage of neutrophils, a decreased percentage of lymphocytes, an increased N:L ratio, a decreased percentage of eosinophils and an increased percentage of monocytes. The influence of cortisol on the parameters of the blood count only exists in the first week because the concentration of cortisol rises sharply and then sharply decreases in the first few days after calving, while the concentration of NEFA rises slightly and is often persistent in the first weeks of lactation, which indicates a negative energy balance . In the study of the influence of metabolic stress indicators on the value of the parameters of the blood count, it was found that the classification of cows according to the values of NEFA and cortisol gives significant differences in the red and white bloodlines, which confirms the importance of peripartum metabolic stress caused by parturition and lactation in the response of the blood count in cows.
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Beretta, Luiza de Lima, Arthur Costa Nascimento, and Diogo Fernandes dos Santos. "Community-acquired pneumococcal meningoencephalitis associated with neurosyphilis in an immunocompetent patient: case report." In XIV Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s1.482.
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Case report: A 28-year-old man with no comorbidities was admitted to our institution with a history of sudden holocranial headache, associated with fever, lowered level of consciousness and meningismus with the need for orotracheal intubation. Computed tomography of the brain was normal and the cerebrospinal fluid (CSF) on 05/28/2022 was yellowish, cloudy, glucose 6.0 mg/ dL, protein 752 mg/dL, cells 25,600 mm³ (neutrophils 92%, lymphocytes 5%), red blood cells 258 mm³, CSF Venereal Disease Research Laboratory (VDRL) 1/8, serum VDRL 1/32, treponemal test positive, human immunodeficiency virus (HIV) negative. Ceftriaxone, ampicillin, and acyclovir were empirically started. Pneumococcus was identified in the culture of CSF and blood cultures on admission and the antibiotic regimen was adequate, maintaining only ceftriaxone. Antibiotic therapy lasted 14 days, he was discharged after 16 days of hospitalization, for outpatient follow-up, with no neurological deficits. Control lumbar puncture on 12/23 revealed clear, colorless CSF, glucose 56 mg/dL, total protein 31.8 mg/dL, no cells or red blood cells, cultures negative. Discussion: Streptococcus pneumoniae is the most common cause of meningitis in adults, in older adults and in the current era, neurosyphilis, is most frequently seen in persons with HIV. There are no similar cases described in the literature. Despite the effectiveness of current antibiotics in clearing bacteria from the CSF, bacterial meningitis continues to cause significant morbidity and mortality worldwide. We describe a rare case of an immunocompetent patient with communityacquired pneumococcal meningoencephalitis associated with neurosyphilis treated with ceftriaxone who did not present sequelae or need for retreatment. Conclusion: It´s a rare cause of meningoencephalitis and has significant morbidity and mortality. More studies are needed regarding susceptibility to meningoencephalitis by multiple germs in immunocompetent patients.Case report: A 28-year-old man with no comorbidities was admitted to our institution with a history of sudden holocranial headache, associated with fever, lowered level of consciousness and meningismus with the need for orotracheal intubation. Computed tomography of the brain was normal and the cerebrospinal fluid (CSF) on 05/28/2022 was yellowish, cloudy, glucose 6.0 mg/ dL, protein 752 mg/dL, cells 25,600 mm³ (neutrophils 92%, lymphocytes 5%), red blood cells 258 mm³, CSF Venereal Disease Research Laboratory (VDRL) 1/8, serum VDRL 1/32, treponemal test positive, human immunodeficiency virus (HIV) negative. Ceftriaxone, ampicillin, and acyclovir were empirically started. Pneumococcus was identified in the culture of CSF and blood cultures on admission and the antibiotic regimen was adequate, maintaining only ceftriaxone. Antibiotic therapy lasted 14 days, he was discharged after 16 days of hospitalization, for outpatient follow-up, with no neurological deficits. Control lumbar puncture on 12/23 revealed clear, colorless CSF, glucose 56 mg/dL, total protein 31.8 mg/dL, no cells or red blood cells, cultures negative. Discussion: Streptococcus pneumoniae is the most common cause of meningitis in adults, in older adults and in the current era, neurosyphilis, is most frequently seen in persons with HIV. There are no similar cases described in the literature. Despite the effectiveness of current antibiotics in clearing bacteria from the CSF, bacterial meningitis continues to cause significant morbidity and mortality worldwide. We describe a rare case of an immunocompetent patient with communityacquired pneumococcal meningoencephalitis associated with neurosyphilis treated with ceftriaxone who did not present sequelae or need for retreatment. Conclusion: It´s a rare cause of meningoencephalitis and has significant morbidity and mortality. More studies are needed regarding susceptibility to meningoencephalitis by multiple germs in immunocompetent patients.
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Bussel, J. "FOR MODULATION AS A MEANS OF ELEVATING THE PLATELET COUNT IN ITP." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644761.
Full textAbstract:
ITP is an autoantibody-mediated disease which would logically be treated by decreasing the level of autoantibody. However, the most exciting developments in understanding the pathophysiology of the thrombocytopenia and its treatment involve a better understanding of the MPS FcR system and ways in which it can be modulated. This work has focussed on phagocytic paralysis or FcR blockade (FcRBl): the slowing of destruction of antibody-coated platelets despite the persistent presence of antibody on the surface of the platelet.Several areas have been explored in learning about the MPS system. Investigation by Kurlander among others have revealed that at least 2 FcR's exist on mononuclear phagocytes: one with high and one with low affinity for monomeric IgG. Study of the high affinity FcR expressed by circulating monocytes, by Schreiber among others, has explored the effect of Danazol to decrease the expression of this FcR. The clinical relevance of this receptor is uncertain however because it is saturated in vitro by physiologic concentrations of IgG. Unkeless defined the properties of the low affinity "immune complex" FcR, expressed on macrophages and neutrophils, via monoclonal antibody 3G8 (see below) which blocks ligand binding to this FcR. The exact roles of these two, and possibly more, FcR's are being explored. Another still unsolved controversy involves whether the interaction Fc portions of antibodies coating particles with FcR's is mediated by a conformational change of the Fc portion or by a multipoint attachment of several Fc parts.Studies by Mollison in the 60's demonstrated that the MPS had a limited capacity for removal of antibody-coated (red) cells. Shulman pursued MPS modulation by exploring the inhibition of thrombocytopenia caused by infusion of ITP plasma into normals. Kelton demonstrated that "compensated" ITP may be caused by a decreased clearance of antibody-coated cells and that the rate of clearance of antibody-coated cells may be correlated with rate of clearance of antibody-coated cells may be correlated with the intrinsic levels of IgG. Stossel investigated FcRBl as a mechanism of effect of corticosteroids and related it clinically. Subsequently intravenous gammaglobulin (IVGG) was introducedas a treatment of ITP and Fehr et al first demonstrated FcRBl as the mechanism of effect of IVGG. Exploration of the mechanism of the FcRBl caused by IVGGled Salama and Mueller-Eckhardt to demonstrate the therapeutic effect of I anti-D, which apparentlycoats RBC with antibody and causes their destruction atthe coats RBC with antibody and causes their destruction at the expense of antibody-coated platelets. A similar degree of FcRBl has been shown for aldometrelated to the development of antibody on RBC.Our studies, including Drs. Clarkson, Kimberly, Nachman, and Unkeless, have focussed on the role of the low affinity or "Immune complex" FcR by using monoclonal antibody 3G8 in vivo. An infusion of 1 mg/kg of 3G8 in chimpanzees caused a reproducible FcRBl demonstrable by a slowing of the destruction of antibody-coated RBC for > 10 days (JEM, 1986). Less effect of 3G8 to inhibit CIC removal was seen using DNA-anti-DNA as the immune complex. In view of the wel1-documented effects of IVGG infusion to create FcRBl, we infused 3G8 into 6 adults with refractory ITP (NEJM, 1986). Specifically these patients were refractory to all forms of conventional therapy including splenectomy, steroids, vinca alkaloid infusion, immunosuppressives and danazol . 3 of the 6 patients had peak platelet responses to >80,000/ul. The other 3 had short-lived platelet increases from 10 to 30,000/ul. These responses confirmed the effect of FcRBl, specifically of the low affinity FcR, to underlie a dramatic platelet increase in therapy of ITP. Surprisingly 3 of the patients had apparent longterm effects of this therapy demonstrable in 2 cases as a maintenance of the platelet count >20,0C0/ul without any further therapy and in 1 case as a clearly enhanced responsiveness to other therapies following 3G8 infusion. Since Natural Killer activity was (transiently) ablated by 3G8 infusion, we speculate that an alternation of regulation of (auto) antibody production by NK cells may be responsible for this effect and that FcR interactions include regulatory roles in addition to their primary function of removal of CIC.