Relevant bibliographies by topics / NGS

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'NGS'

Author: Grafiati
Published: 4 June 2021
Last updated: 29 March 2025

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Journal articles on the topic "NGS"

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Tatebale, Rivaldo, Orbanus Naharia, and Helen J. Lawalata. "Isolation and Identification of Lactic Acid Bacteria from Red Dragon Fruit (Hylocereus polyrhicus) as Exopolysaccharide Producers." Indonesian Biodiversity Journal 5, no. 1 (April 28, 2024): 8–19. https://doi.org/10.53682/ibj.v5i1.7730.

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Cherry tomatoes are a type of Red Dragon Fruit that has various benefits, including lowering cholesterol levels, preventing colon cancer, and strengthening the working power of muscles. Isolation of LAB isolated from Dragon Fruit as a production material for EPS. This study aims to isolate and identify LAB as a producer of EPS from red dragon fruit (Hylocereus polyrhiruz), which can produce exopolysaccharides. This research uses a descriptive research method. Data from experimental research in the laboratory obtained 10 LAB isolates, namely isolates NG1, NG2, NG3, NG4, NG5, NG6, NG7, NG8, NG9 and NG10. Based on the identification results, isolate NG1 has similarities with the genus Lactococcus (spherical, gram-positive, nonmotile, non-spore). While isolates NG2, NG3, NG4, NG5, NG6, NG7, NG8, NG9 and NG10 have similarities with the genus Lactobacillus (rod form, nonmotile, gram-positive). Based on the morphological characteristics, which are gram-positive, catalase-negative and non-spore. The ten isolates of lactic acid bacteria are capable of producing EPS. these are the results of EPS acquisition, namely: NG1(152.1 mg/L), NG2(127.9 mg/L), NG3(134.6 mg/L), NG4(130.9 mg/L), NG5(137 mg/L), NG6(139.2 mg/L), NG7(204.9 mg/L), NG8(156.2 mg/L), NG9(136.4 mg/L), and NG10 (157, 3 mg/L). The highest amount of EPS was isolated NG7 at 204.9 mg/L. Meanwhile, the lowest EPS was isolated NG4 at 130.9 mg/L.
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Tunis, Sean. "Reimbursing NGS Testing." Clinical OMICs 2, no. 1 (January 2015): 21–23. http://dx.doi.org/10.1089/clinomi.02.01.08.

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Zemlo, Tamara. "Democratizing NGS Platforms." Genetic Engineering & Biotechnology News 32, no. 5 (March 2012): 16–18. http://dx.doi.org/10.1089/gen.32.5.05.

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Tunis, Sean. "Reimbursing NGS Testing." Genetic Engineering & Biotechnology News 35, no. 3 (February 2015): 5–6. http://dx.doi.org/10.1089/gen.35.03.03.

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Kim, Annette S., Angela N. Bartley, Julia A. Bridge, Kelly Devereaux, A. John Iafrate, Lawrence Jennings, Suzanne Kamel-Reid, et al. "31. The PT alphabet soup: LDT, FDA, NGS, non-NGS, @#$!%." Cancer Genetics 233-234 (April 2019): S13. http://dx.doi.org/10.1016/j.cancergen.2019.04.037.

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Szabo, Kathrin, Burkhard Malorny, and Manfred Stoyke. "Etablierung der § 64 LFGB Arbeitsgruppen „NGS – Bakteriencharakterisierung“ und „NGS – Speziesidentifizierung“." Journal of Consumer Protection and Food Safety 15, no. 1 (October 22, 2019): 85–89. http://dx.doi.org/10.1007/s00003-019-01255-z.

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Zusammenfassung Das Next-Generation Sequencing (NGS) besitzt großes Potential im Bereich der Lebensmittelsicherheit und der Authentizitätsprüfung von Lebensmitteln. Die Gesamtgenomsequenzierung mikrobieller Genome kombiniert mit bioinformatischen Auswerteprogrammen ersetzt zunehmend die klassischen Typisierungsmethoden und gilt aufgrund ihres außerordentlichen Auflösungsvermögen mittlerweile als Methode der Wahl im Rahmen von Ausbruchsuntersuchungen. Zur Authentizitätskontrolle z. B. von Fleisch- und Fischproben finden NGS-Methoden als Metabarcoding immer häufiger Anwendung, um Täuschung und Irreführung bis hin zu Lebensmittelbetrug aufzudecken. Einige Untersuchungsbehörden verfügen bereits über die NGS-Technologie und setzen diese auch erfolgreich ein, weitere Einrichtungen werden folgen. Um den mit der Lebensmittelüberwachung betrauten Behörden validierte, leistungsfähige und standardisierte NGS-Methoden zur Verfügung zu stellen, ist eine Aufnahme dieser Methoden in die „Amtliche Sammlung von Verfahren zur Probenahme und Untersuchung von Lebensmitteln“ (ASU) durch die Gründung zweier neuer § 64 LFGB Arbeitsgruppen mit unterschiedlichen thematischen Schwerpunkten vorgesehen. Die Arbeitsgruppe „NGS – Bakteriencharakterisierung“ bearbeitet NGS-Verfahren für die Sequenzierung bakterieller Erreger im Rahmen von Ausbruchsuntersuchungen. Die Arbeitsgruppe „NGS – Speziesidentifizierung“ beschäftigt sich mit NGS-Methoden zur Tierartendifferenzierung in Lebensmitteln. Am 6. März 2019 fand das erste Treffen der Arbeitsgruppe „NGS – Speziesidentifizierung“ und am folgenden Tag, dem 7. März 2019 das der Arbeitsgruppe „NGS – Bakteriencharakterisierung“ auf Einladung des Bundesamts für Verbraucherschutz und Lebensmittelsicherheit (BVL) in Berlin statt. Auf den Sitzungen wurden durch die Mitglieder der Gruppen NGS-Methoden zur Bakteriencharakterisierung bzw. zur Tierartendifferenzierung in Lebensmitteln vorgestellt. Anschließend diskutierten die Mitglieder die ersten thematischen Schwerpunkte der Methodenentwicklung, Validierungskonzepte, Qualitätskontrollmaßnahmen und den Einsatz dieser Methoden in der Lebensmittelüberwachung. Es wurde beschlossen, durch laborübergreifende Vorringversuche die Vergleichbarkeit der verschiedenen NGS-Technologien zu ermitteln sowie die entsprechenden Auswerteparameter, Qualitätskriterien und Validierungsparameter für eine laborübergreifende Validierungsstudie zu erarbeiten.
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Veldman, Abigail, Mensiena B. G. Kiewiet, Margaretha Rebecca Heiner-Fokkema, Marcel R. Nelen, Richard J. Sinke, Birgit Sikkema-Raddatz, Els Voorhoeve, et al. "Towards Next-Generation Sequencing (NGS)-Based Newborn Screening: A Technical Study to Prepare for the Challenges Ahead." International Journal of Neonatal Screening 8, no. 1 (February 24, 2022): 17. http://dx.doi.org/10.3390/ijns8010017.

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Newborn screening (NBS) aims to identify neonates with severe conditions for whom immediate treatment is required. Currently, a biochemistry-first approach is used to identify these disorders, which are predominantly inherited meta1bolic disorders (IMD). Next-generation sequencing (NGS) is expected to have some advantages over the current approach, for example the ability to detect IMDs that meet all screening criteria but lack an identifiable biochemical footprint. We have now designed a technical study to explore the use of NGS techniques as a first-tier approach in NBS. Here, we describe the aim and set-up of the NGS-first for the NBS (NGSf4NBS) project, which will proceed in three steps. In Step 1, we will identify IMDs eligible for NGS-first testing, based on treatability. In Step 2, we will investigate the feasibility, limitations and comparability of different technical NGS approaches and analysis workflows for NBS, eventually aiming to develop a rapid NGS-based workflow. Finally, in Step 3, we will prepare for the incorporation of this workflow into the existing Dutch NBS program and propose a protocol for referral of a child after a positive NGS test result. The results of this study will be the basis for an additional analytical route within NBS that will be further studied for its applicability within the NBS program, e.g., regarding the ethical, legal, financial and social implications.
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Ardeshirdavani, Amin, Erika Souche, Luc Dehaspe, Jeroen Van Houdt, Joris Vermeesch, and Yves Moreau. "NGS-Logistics : Federated analysis of NGS sequence variants across multiple locations." Genome Medicine 6, no. 9 (2014): 71. http://dx.doi.org/10.1186/preaccept-3696327041308731.

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May, Ali, Sanne Abeln, Mark J. Buijs, Jaap Heringa, Wim Crielaard, and Bernd W. Brandt. "NGS-eval: NGS Error analysis and novel sequence VAriant detection tooL." Nucleic Acids Research 43, W1 (April 15, 2015): W301—W305. http://dx.doi.org/10.1093/nar/gkv346.

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Ravasio, Viola, Marco Ritelli, Andrea Legati, and Edoardo Giacopuzzi. "GARFIELD-NGS: Genomic vARiants FIltering by dEep Learning moDels in NGS." Bioinformatics 34, no. 17 (April 14, 2018): 3038–40. http://dx.doi.org/10.1093/bioinformatics/bty303.

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Dissertations / Theses on the topic "NGS"

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Toledo, Rodrigo Atique Ferraz de. "Identificação e análise funcional de mutação associadas às craniossinostoses." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/41/41131/tde-20122016-090151/.

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As craniossinostoses são malformações craniofaciais caracterizadas pelo fechamento precoce de uma ou mais suturas cranianas. Elas são doenças congênitas e são causadas por mutações em diversos genes devido ao grande número de vias envolvidas na formação e manutenção das suturas cranianas. Embora mutações em 53 genes já tenham sido descritas o conhecimento da genética e da patofisiologia das craniossinostoses ainda é incompleto. Nesse trabalho tivemos como objetivo a identificação de novas mutações associadas às craniossinostoses bem como o aprofundamento do conhecimento sobre a atuação dessas mutações em células humanas por meio de estudos funcionais. Para identificarmos novas mutações utilizamos metodologias de sequenciamento em larga escala conhecidas como sequenciamento de noiva geração (NGS). Identificamos a mutação causal em uma paciente proveniente de um casamento consanguíneo portadora da síndrome de Raine (p.P496L em FAM20C). Também delimitamos a poucas mutações candidatas outros onze casos atípicos de craniossinostose. Por fim estudamos os efeitos de diferentes FGFs sobre o comportamento de células com a mutação mais comum causadora da S. de Apert, p.S252W em FGFR2. Descobrimos que os FGFs10 e 19 têm ações distintas sobre o perfil transcricional e sobre a taxa de proliferação de células mutantes. Também descobrimos que as células tronco mesenquimais e as células fibroblastóides têm comportamentos distintos ao serem tratadas com FGF19. Os resultados aqui apresentados serão de grande serventia para o melhor delineamento da biologia das suturas cranianas e da patofisiologia das craniossinostoses
Craniosynostosis are craniofacial malformations defined by early closure of the cranial sutures. They are congenital diseases caused by mutations in several genes due to the diversity of pathways involved in the development and maintenance of the cranial sutures. Even though 53 genes have already been linked to various forms of craniosynostosis, the knowledge about the genetics and pathophysiology is incomplete. In this work we aimed to identify new mutations associated with craniosynostosis as well as to further the knowledge of how those mutations act in human cells. To identify new variants associated with craniosynostosis we used large scale sequencing techniques known as next generation sequencing (NGS). We were able to identify the causal mutation in one patient from a consanguineous marriage with Raine syndrome (p.P496L in FAM20C). We also were able to elect candidate mutations in other eleven cases of atypical craniosynostosis. Lastly, we studied the effects of different FGFs over the behavior of human cells harboring the most common Apert syndrome mutation, p.S252W in FGFR2. We discovered that FGFs 10 and 19 have different effects over the transcriptional profile and proliferation rate of mutant cells. We also found that FGF19 have opposite effects in mesenchymal stem cells and fibroblastoid cells osteogenic differentiation. The results shown here will be of great service to better understand the biology of cranial suture and the pathophysiology of craniosynostosis
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Riou, Raphaelle. "Contribution à l'étude du rôle des cellules Natural Killer dans le contrôle de l'infection à cytomégalovirus." Thesis, Nantes, 2016. http://www.theses.fr/2016NANT1002/document.

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Les cellules NK, membres de l’immunité innée, sont impliquées dans le contrôle des infections virales et notamment l’infection à cytomégalovirus (CMV). Généralement bien tolérée chez l’individu immunocompétent, l’infection à CMV demeure associée à une forte morbidité chez les individus dont le système immunitaire est compromis (patients transplantés, coinfectés par le VIH) ou immature (fœtus et nouveaux nés). Lors de l’infection par le CMV, une relation étroite s’établit entre le système immunitaire et le virus. Celle-ci génère en effet une forte mobilisation, associée à un profond remodelage, de différents compartiments immuns. Au cours de ce travail de thèse, nous nous sommes intéressées à l’équilibre qui s’instaure entre le virus et le système immunitaire. Dans une première approche menée in vitro, nous avons exploré le rôle de différentes sous-populations NK dont les cellules NK NKG2C+, caractéristiques de l’infection à CMV, en réponse à des cellules endothéliales, isolées à partir de donneurs de rein, infectées par le CMV. Ensuite, une cohorte rare d’adultes immunocompétents souffrant d’une primo-infection symptomatique à CMV nous a permis d’étudier la course naturelle de l’infection à CMV. Cette approche ex vivo a constitué d’une part en l’analyse du polymorphisme de la réponse de l’hôte au virus, par l’étude phénotypique et transcriptomique non seulement de la réponse NK mais aussi d’autres effecteurs lymphocytaires. D’autre part, nous avons exploré l’impact du polymorphisme génétique viral, par le séquençage par NGS d’isolats cliniques du CMV, sur le pouvoir pathogène du virus. L’ensemble de ces travaux devrait contribuer à la meilleure compréhension du rôle des cellules NK dans le contrôle de l’infection à CMV
NK cells are innate lymphocyte effectors involved in the control of viral infections and particularly cytomegalovirus (CMV) infection. Usually well tolerated in immunocompetent individuals, CMV infection remains life life-threatening in immunosuppressed patients, as transplant recipients or HIV-infected patients, or for fetuses in case of congenital infection. Upon primary infection, CMV establishes a close relationship with the immune system. CMV infection is known to drive an important immune response and to deeply imprint several immune compartments. In this present work, we focused on the host-virus balance that takes place upon infection. Through a first in vitro approach, we investigated the role of different NK cell subpopulations, including NKG2C+ NK cells which represent one of the hallmarks of CMV infection, in response to CMVinfected endothelial cells isolated from kidney donors. Then, an ex vivo approach was conducted in a cohort of immunocompetent adults diagnosed with symptomatic primary CMV infection. On one hand, our aim was to explore the host immune response polymorphism, through phenotypic and transcriptomic analyses of lymphocyte responses. On the other hand, we investigated the viral genome polymorphism, through NGS sequencing of clinical CMV isolates, which could modulate the viral pathogenicity. Taken together, these findings should contribute to the better understanding of the role of NK cells during the course of CMV infection
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Prieto, Barja Pablo 1986. "NGS applications in genome evolution and adaptation : A reproducible approach to NGS data analysis and integration." Doctoral thesis, Universitat Pompeu Fabra, 2017. http://hdl.handle.net/10803/565601.

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In this PhD I have used NGS technologies in different organisms and scenarios such as in ENCODE, comparing the conservation and evolution of long non-coding RNA sequences between human and mouse, using experimental evidences from genome, transcriptome and chromatin. A similar approach was followed in other organisms such as the mesoamerican common bean and in chicken. Other analysis carried with NGS data involved the well known parasite, Leishmania Donovani, the causative agent of Leishmaniasis. I used NGS data obtained from genome and transcriptome to study the fate of its genome in survival strategies for adaptation and long term evolution. All this work was approached while working in tools and strategies to efficiently design and implement the bioinformatics analysis also known as pipelines or workflows, in order to make them easy to use, easily deployable, accessible and highly performing. This work has provided several strategies in order to avoid lack of reproducibility and inconsistency in scientific research with real biological applications towards sequence analysis and genome evolution.
En aquest doctorat he utilitzat tecnologies NGS en diferents organismes i projectes com l'ENCODE, comparant la conservació i evolució de seqüències de RNA llargs no codificant entre el ratolí i l'humà, utilitzant evidències experimentals del genoma, transcriptoma i cromatina. He seguit una estratègia similar en altres organismes com són la mongeta mesoamericana i el pollastre. En altres anàlisis he hagut d'utilitzar dades NGS en l'estudi del conegut paràsit leishmània Donovani, l'agent causatiu de la malaltia Leishmaniosis. Utilitzant dades NGS obtingudes del genoma i transcriptoma he estudiat les conseqüències del genoma en estratègies d'adaptació i evolució a llarg termini. Aquest treball es va realitzar mentre treballava en eines i estratègies per dissenyar eficientment i implementar els anàlisis bioinformàtics coneguts com a diagrames de treball, per tal de fer-los fàcils d'utilitzar, fàcilment realitzables, accessibles i amb un alt rendiment. Aquest treball present diverses estratègies per tal d'evitar la falta de reproductibilitat i consistència en la investigació científica amb aplicacions reals a la biologia de l'anàlisi de seqüències i evolució de genomes.
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Matocha, Petr. "Efektivní hledání překryvů u NGS dat." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2017. http://www.nusl.cz/ntk/nusl-363811.

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The main theme of this work is the detection of overlaps in NGS data. The work contains an overview of NGS sequencing technologies that are the source of NGS data. In the thesis, the problem of overlapping detection is generally defined. Next, an overview of the available algorithms and approaches for detecting overlaps in NGS data is created. Principles of these algorithms are described herein. In the second part of this work a suitable tool for detecting approximate overlaps in NGS data is designed and its implementation is described herein. In conclusion, the experiments performed with this tool and the conclusions that follow are summarized and described.
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Alic, Andrei Stefan. "Improved Error Correction of NGS Data." Doctoral thesis, Universitat Politècnica de València, 2016. http://hdl.handle.net/10251/67630.

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[EN] The work done for this doctorate thesis focuses on error correction of Next Generation Sequencing (NGS) data in the context of High Performance Computing (HPC). Due to the reduction in sequencing cost, the increasing output of the sequencers and the advancements in the biological and medical sciences, the amount of NGS data has increased tremendously. Humans alone are not able to keep pace with this explosion of information, therefore computers must assist them to ease the handle of the deluge of information generated by the sequencing machines. Since NGS is no longer just a research topic (used in clinical routine to detect cancer mutations, for instance), requirements in performance and accuracy are more stringent. For sequencing to be useful outside research, the analysis software must work accurately and fast. This is where HPC comes into play. NGS processing tools should leverage the full potential of multi-core and even distributed computing, as those platforms are extensively available. Moreover, as the performance of the individual core has hit a barrier, current computing tendencies focus on adding more cores and explicitly split the computation to take advantage of them. This thesis starts with a deep analysis of all these problems in a general and comprehensive way (to reach out to a very wide audience), in the form of an exhaustive and objective review of the NGS error correction field. We dedicate a chapter to this topic to introduce the reader gradually and gently into the world of sequencing. It presents real problems and applications of NGS that demonstrate the impact this technology has on science. The review results in the following conclusions: the need of understanding of the specificities of NGS data samples (given the high variety of technologies and features) and the need of flexible, efficient and accurate tools for error correction as a preliminary step of any NGS postprocessing. As a result of the explosion of NGS data, we introduce MuffinInfo. It is a piece of software capable of extracting information from the raw data produced by the sequencer to help the user understand the data. MuffinInfo uses HTML5, therefore it runs in almost any software and hardware environment. It supports custom statistics to mould itself to specific requirements. MuffinInfo can reload the results of a run which are stored in JSON format for easier integration with third party applications. Finally, our application uses threads to perform the calculations, to load the data from the disk and to handle the UI. In continuation to our research and as a result of the single core performance limitation, we leverage the power of multi-core computers to develop a new error correction tool. The error correction of the NGS data is normally the first step of any analysis targeting NGS. As we conclude from the review performed within the frame of this thesis, many projects in different real-life applications have opted for this step before further analysis. In this sense, we propose MuffinEC, a multi-technology (Illumina, Roche 454, Ion Torrent and PacBio -experimental), any-type-of-error handling (mismatches, deletions insertions and unknown values) corrector. It surpasses other similar software by providing higher accuracy (demonstrated by three type of tests) and using less computational resources. It follows a multi-steps approach that starts by grouping all the reads using a k-mers based metric. Next, it employs the powerful Smith-Waterman algorithm to refine the groups and generate Multiple Sequence Alignments (MSAs). These MSAs are corrected by taking each column and looking for the correct base, determined by a user-adjustable percentage. This manuscript is structured in chapters based on material that has been previously published in prestigious journals indexed by the Journal of Citation Reports (on outstanding positions) and relevant congresses.
[ES] El trabajo realizado en el marco de esta tesis doctoral se centra en la corrección de errores en datos provenientes de técnicas NGS utilizando técnicas de computación intensiva. Debido a la reducción de costes y el incremento en las prestaciones de los secuenciadores, la cantidad de datos disponibles en NGS se ha incrementado notablemente. La utilización de computadores en el análisis de estas muestras se hace imprescindible para poder dar respuesta a la avalancha de información generada por estas técnicas. El uso de NGS transciende la investigación con numerosos ejemplos de uso clínico y agronómico, por lo que aparecen nuevas necesidades en cuanto al tiempo de proceso y la fiabilidad de los resultados. Para maximizar su aplicabilidad clínica, las técnicas de proceso de datos de NGS deben acelerarse y producir datos más precisos. En este contexto es en el que las técnicas de comptuación intensiva juegan un papel relevante. En la actualidad, es común disponer de computadores con varios núcleos de proceso e incluso utilizar múltiples computadores mediante técnicas de computación paralela distribuida. Las tendencias actuales hacia arquitecturas con un mayor número de núcleos ponen de manifiesto que es ésta una aproximación relevante. Esta tesis comienza con un análisis de los problemas fundamentales del proceso de datos en NGS de forma general y adaptado para su comprensión por una amplia audiencia, a través de una exhaustiva revisión del estado del arte en la corrección de datos de NGS. Esta revisión introduce gradualmente al lector en las técnicas de secuenciación masiva, presentando problemas y aplicaciones reales de las técnicas de NGS, destacando el impacto de esta tecnología en ciencia. De este estudio se concluyen dos ideas principales: La necesidad de analizar de forma adecuada las características de los datos de NGS, atendiendo a la enorme variedad intrínseca que tienen las diferentes técnicas de NGS; y la necesidad de disponer de una herramienta versátil, eficiente y precisa para la corrección de errores. En el contexto del análisis de datos, la tesis presenta MuffinInfo. La herramienta MuffinInfo es una aplicación software implementada mediante HTML5. MuffinInfo obtiene información relevante de datos crudos de NGS para favorecer el entendimiento de sus características y la aplicación de técnicas de corrección de errores, soportando además la extensión mediante funciones que implementen estadísticos definidos por el usuario. MuffinInfo almacena los resultados del proceso en ficheros JSON. Al usar HTML5, MuffinInfo puede funcionar en casi cualquier entorno hardware y software. La herramienta está implementada aprovechando múltiples hilos de ejecución por la gestión del interfaz. La segunda conclusión del análisis del estado del arte nos lleva a la oportunidad de aplicar de forma extensiva técnicas de computación de altas prestaciones en la corrección de errores para desarrollar una herramienta que soporte múltiples tecnologías (Illumina, Roche 454, Ion Torrent y experimentalmente PacBio). La herramienta propuesta (MuffinEC), soporta diferentes tipos de errores (sustituciones, indels y valores desconocidos). MuffinEC supera los resultados obtenidos por las herramientas existentes en este ámbito. Ofrece una mejor tasa de corrección, en un tiempo muy inferior y utilizando menos recursos, lo que facilita además su aplicación en muestras de mayor tamaño en computadores convencionales. MuffinEC utiliza una aproximación basada en etapas multiples. Primero agrupa todas las secuencias utilizando la métrica de los k-mers. En segundo lugar realiza un refinamiento de los grupos mediante el alineamiento con Smith-Waterman, generando contigs. Estos contigs resultan de la corrección por columnas de atendiendo a la frecuencia individual de cada base. La tesis se estructura por capítulos cuya base ha sido previamente publicada en revistas indexadas en posiciones dest
[CAT] El treball realitzat en el marc d'aquesta tesi doctoral se centra en la correcció d'errors en dades provinents de tècniques de NGS utilitzant tècniques de computació intensiva. A causa de la reducció de costos i l'increment en les prestacions dels seqüenciadors, la quantitat de dades disponibles a NGS s'ha incrementat notablement. La utilització de computadors en l'anàlisi d'aquestes mostres es fa imprescindible per poder donar resposta a l'allau d'informació generada per aquestes tècniques. L'ús de NGS transcendeix la investigació amb nombrosos exemples d'ús clínic i agronòmic, per la qual cosa apareixen noves necessitats quant al temps de procés i la fiabilitat dels resultats. Per a maximitzar la seua aplicabilitat clínica, les tècniques de procés de dades de NGS han d'accelerar-se i produir dades més precises. En este context és en el que les tècniques de comptuación intensiva juguen un paper rellevant. En l'actualitat, és comú disposar de computadors amb diversos nuclis de procés i inclús utilitzar múltiples computadors per mitjà de tècniques de computació paral·lela distribuïda. Les tendències actuals cap a arquitectures amb un nombre més gran de nuclis posen de manifest que és esta una aproximació rellevant. Aquesta tesi comença amb una anàlisi dels problemes fonamentals del procés de dades en NGS de forma general i adaptat per a la seua comprensió per una àmplia audiència, a través d'una exhaustiva revisió de l'estat de l'art en la correcció de dades de NGS. Esta revisió introduïx gradualment al lector en les tècniques de seqüenciació massiva, presentant problemes i aplicacions reals de les tècniques de NGS, destacant l'impacte d'esta tecnologia en ciència. D'este estudi es conclouen dos idees principals: La necessitat d'analitzar de forma adequada les característiques de les dades de NGS, atenent a l'enorme varietat intrínseca que tenen les diferents tècniques de NGS; i la necessitat de disposar d'una ferramenta versàtil, eficient i precisa per a la correcció d'errors. En el context de l'anàlisi de dades, la tesi presenta MuffinInfo. La ferramenta MuffinInfo és una aplicació programari implementada per mitjà de HTML5. MuffinInfo obté informació rellevant de dades crues de NGS per a afavorir l'enteniment de les seues característiques i l'aplicació de tècniques de correcció d'errors, suportant a més l'extensió per mitjà de funcions que implementen estadístics definits per l'usuari. MuffinInfo emmagatzema els resultats del procés en fitxers JSON. A l'usar HTML5, MuffinInfo pot funcionar en gairebé qualsevol entorn maquinari i programari. La ferramenta està implementada aprofitant múltiples fils d'execució per la gestió de l'interfície. La segona conclusió de l'anàlisi de l'estat de l'art ens porta a l'oportunitat d'aplicar de forma extensiva tècniques de computació d'altes prestacions en la correcció d'errors per a desenrotllar una ferramenta que suport múltiples tecnologies (Illumina, Roche 454, Ió Torrent i experimentalment PacBio). La ferramenta proposada (MuffinEC), suporta diferents tipus d'errors (substitucions, indels i valors desconeguts). MuffinEC supera els resultats obtinguts per les ferramentes existents en este àmbit. Oferix una millor taxa de correcció, en un temps molt inferior i utilitzant menys recursos, la qual cosa facilita a més la seua aplicació en mostres més gran en computadors convencionals. MuffinEC utilitza una aproximació basada en etapes multiples. Primer agrupa totes les seqüències utilitzant la mètrica dels k-mers. En segon lloc realitza un refinament dels grups per mitjà de l'alineament amb Smith-Waterman, generant contigs. Estos contigs resulten de la correcció per columnes d'atenent a la freqüència individual de cada base. La tesi s'estructura per capítols la base de la qual ha sigut prèviament publicada en revistes indexades en posicions destacades de l'índex del Journal of Citation Repor
Alic, AS. (2016). Improved Error Correction of NGS Data [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/67630
TESIS
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6

Mohamed, Bashir. "NGS-baserad metod för fetal blodgruppstypning." Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-106208.

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Hemolytic disease of fetus or newborn (HDFN) är en komplikation där foster eller nyföddas erytrocyter förstörs för tidigt. HDFN uppstår när det föreligger blodgruppsinkompatibilitet mellan moder och barnet. Komplikationerna/ symptomen kan variera allt från mildare symptom till fosterdöd. HDFN orsakas framförallt av antikropp D (RhD-immunisering) och på grund av detta utförs det typning av fetalt RhD i maternell plasma. Utöver RhD-immuniseringar kan svåra fall av HDFN ibland orsakas av andra blodgruppssystem som c (Rh) och K (Kell). Fetal RhD-typning görs idag som screening på alla RhD-negativa gravida mödrar och utförs i Stockholm och Lund. Metoden som används är baserad på realtids-PCR och har använts sedan 2009. Fetal typning av c och K görs när höga titrar av anti-c respektive anti-K påvisas i mammans plasma. För fetal typning av c och K skickas prover från Huddinge till Lund respektive Amsterdam. Motsvarande immunisering mot erytrocytantigen kan även bildas mot trombocytantigen främst Human Platelet Antigene (HPA-1a) som uttrycks hos fostret och leda till fetal neonatal alloimmun trombocytopeni (FNAIT). En next generation sequencing (NGS) baserad metod för genomisktypning av alla kliniskt relevanta blodgruppssystem finns idag på Karolinska universitetssjukhuset. Denna metod är dock inte anpassad för fetal blodgruppstypning där cell-fritt foster- DNA (cffDNA) analyseras. Syftet med det här projektet var att optimera designade oligonukleotider (oligos) anpassade för fetal blodgruppstypning med NGS-metodik. Design av oligos utfördes innan examensarbetet påbörjades. Dessa inkluderade primer- par som amplifierar upp alla blodgruppssystem som kan orsaka HDFN och FNAIT. Utöver det inkluderade designade oligos markörer som amelogenin-XY (Amel-XY) som är könsspecifika markörer och insertion/deletion markörer (Indel-markörer). Med Amel-XY markörerna kan könsspecifika Y-genen påvisas i provet, vilket kommer att fungera som en kontroll på att foster-DNA har analyserats (fungerar endast vid manligt foster). Designade Indel-markörer består av 2 - 3 bp insertioner/deletioner där olika markörerna används till att skilja DNA från foster och modern i framtiden. De designade oligonukleotiderna undersöktes för om de ger tillräckligt bra amplifiering av sökta sekvenser med NGS genom att analysera två slumpmässigt valda försöksprover (genomisk DNA 2 ng/ μL) från en man och en kvinna. Resultatet visade att tillräckligt bra amplifieringar/signaler erhålls från samtliga markörer som ingår i studien. Den totala signalen för samtliga markörer blev 114234 läsningar (”reads”) vilket gav ett medelvärde på 3939 läsningar. För att mäta amplifieringsförmågan hos varje enskild markör beräknades procent av medelvärdet på 3939 läsningar. En signal på 20 – 180 % av medelvärdet ansågs vara godkänt för varje markör. Alla undersökta primer-markörer uppvisade en signal på minst 45 % av medelvärdet vilket var godkänt (godkänt intervall 20 – 180 %). Efter optimering av oligos som ger tillräckligt bra amplifiering genotypades 32 slumpmässigt valda DNA prover från 21 män och 11 kvinnor med hjälp av NGS. Efter NGS-körning och analys av fastQ-filer med hjälp av mjukvaruprogrammet R erhölls resultat i form av signaler. Signaler för respektive markör över 50 läsningar ansågs vara en positiv signal det vill säga att den sökta genen har påvisats. Signal-värde under 50 innebar att det förekom bakgrundssignal då önskat signal-värde vid avsaknad av sökt gensekvens är 0 (åsatt gränsvärde <1 % av högst uppmätta positiva signalen för varje markör). Samtliga undersökta markörer visade låg bakgrundssignal (<1 % ). Bakgrundssignalen beräknades för att veta vilken tillförlitlig nivå varje markör ska ligga på för att kunna detektera känsligt foster-DNA i framtiden. Resultaten i denna studie visade att de optimerade oligos kan användas till genotypning av cffDNA i framtiden eftersom markörerna uppvisade låga bakgrundssignaler vilket indikerar att metoden är tillräckligt effektiv för att kunna detektera känsligt foster-DNA i framtiden.
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7

Gorgé, Olivier. "Diagénèse de l’ADN bactérien et analyses métagénomiques de pathologies bactériennes du passé." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLS572/document.

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Cette étude a pour objet la mise en évidence de traces d'ADN bactérien pathogène dans des échantillons animaux et humains anciens, et ainsi améliorer les connaissances sur l'évolution des maladies au cours du temps. En parallèle, nous avons étudié les phénomènes de dégradation de l'ADN dans le sol sur des cadavres de souris enterrées après avoir été contaminées par des bactéries non pathogènes. Cette étude des processus taphonomiques s'est étalée sur trois ans et a permis de montrer une disparition rapide des bactéries simulantes, remplacé par l'ADN des bactéries du sol, qui colonisent rapidement la dépouille et dégradent tant l'ADN endogène (murin) qu'exogène (bactérien). Cette disparition rapide explique la grande difficulté à mettre en évidence des pathogènes dans des échantillons anciens, à de rares exceptions près. Notre étude n'a pas permis de détecter d'agents pathogènes particuliers dans les échantillons que nous avons étudié, mais nous avons mis en évidence l'intérêt d'analyser certains types de restes pour accéder à une information génétique préservée. Le tartre dentaire indique est un bon indicateur de la flore buccale de l'hôte et les kystes calcifiés assurent une bonne préservation de l'ADN endogène, moins soumis à contamination et digestion par les bactéries de l'environnement. Les kystes présentent en règle générale une teneur en ADN endogène supérieure à tous les autres tissus étudiés
The aim of this study was the identification of pathogenic bacterial DNA traces in ancient animal and human samples, and thus improve knowledge of past diseases that affect humankind over time. In parallel, we studied the DNA degradation phenomena in the soil on the buried corpses of mice after being contaminated by non-pathogenic bacteria. This study of taphonomic processes was spread over three years and has shown a rapid disappearance of simulant bacteria, replaced with the DNA of soil bacteria that colonize the body quickly after burial and degrade both the endogenous DNA (murine) that exogenous (bacteria). This quick degradation can explain the high difficulty to detect and identify bacterial pathogens in old samples, with very few exceptions. Despite the fact in our study we were not able to detect specific pathogens in the samples we have studied, we have shown the interest to analyze certain types of remnants to access preserved and informative genetic data. Dental calculus is a good indicator of the oral flora of the host and calcified cysts ensure good preservation of the endogenous DNA, less subject to contamination and digestion by bacteria from the environment. Cysts generally have an endogenous DNA content higher than all other tissues examined
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8

Hammami, Ali. "La sécurité des futures architectures convergentes pour des services personnalisés : aspect architectural et protocolaire." Thesis, Paris, ENST, 2013. http://www.theses.fr/2013ENST0039/document.

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L’émergence et l’évolution des réseaux de nouvelles génération (NGN) a soulevé plusieurs défis surtout en termes d’hétérogénéité, de mobilité et de sécurité. En effet, l’utilisateur est capable, dans un tel environnement, d’avoir accès à plusieurs réseaux, à travers différents terminaux, avec un choix vaste de services fournis par différents fournisseurs. De plus, les utilisateurs finaux demandent à être constamment connectés n’importe où, n’importe quand et n’importe comment. Ils désirent également avoir un accès sécurisé à leurs services à travers une session dynamique, seamless et continue selon leurs préférences et la QoS demandée. Dans ce contexte, la sécurité représente une composante majeure. Face à cette session user-centric sécurisée, plusieurs défis se posent. L’environnement est de plus en plus ouvert, de multiples services ne sont pas connus d’avance et nous avons une diversité de communications entre les services et les utilisateurs. L’hétérogénéité des ressources (terminaux, réseaux et services) impliquées dans la session de l’utilisateur accentue la complexité des tâches de sécurité. Les différentes déclinaisons de mobilité (mobilité de l’utilisateur, mobilité du terminal, mobilité du réseau et mobilité du service) modifient la session user-centric que l’on veut unique, sécurisée et seamless avec la délivrance d’un service continu
The emergence and evolution of Next Generation Networks (NGN) have raised several challenges mainly in terms of heterogeneity, mobility and security. In fact, the user is able, in such environment, to have access to many networks, via multiple devices, with a vast choice of services offered by different providers. Furthermore, end-users claim to be constantly connected anywhere, anytime and anyhow. Besides, they want to have a secure access to their services through a dynamic, seamless and continuous session according to their preferences and the desired QoS. In this context, security represents an important concern. In fact, this user-centric session should obviously be secured. However, many challenges arise. In such environment, system boundaries, which were well delimited, become increasingly open. Indeed, there are multiple services which are unknown in advance and multiple communications between services and with users. Besides, heterogeneity of involved resources (terminals, networks and services) in the user session increases the complexity of security tasks. In addition, the different types of mobility (user, terminal, network and service mobility) affect the user-centric session that should be unique, secure and seamless and ensure continuity of services
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9

Nowoshilow, Sergej. "Transcriptome analysis of axolotl spinal cord and limb regeneration." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-205953.

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Regeneration is a relatively widespread phenomenon in nature, although different organisms exhibit different abilities to reconstitute missing structures. Due to the diversity in the extent of damage the organisms can repair it has been debated for a long time whether those abilities are evolutionary traits that arose independently in multiple organisms or whether they represent a by-product of more basic processes. To date, due to constant increase in the amount of available genomic information this question can be approached by means of comparative genomics by comparing several taxa that have different regenerative capabilities. Two relatively closely related salamander species, newt, Notophthalmus viridescens, and the Mexican axolotl, Ambystoma mexicanum, offer a unique opportunity to compare two organisms with well-known regenerative capabilities. Despite their importance for regeneration research, relatively little sequence information was available until recently, owing mainly to the large sizes of the respective genomes. In this work I aimed to create a comprehensive transcriptome assembly of the axolotl by sequencing and then assembling the sequence data from a number of tissues and developmental stages. I also incorporated available sequence information that mostly comes from cDNA libraries sequenced previously. I assessed the completeness of the transcriptome by comparing it to a set of available axolotl sequences and found that 96% of those have homologs in the assembly. Additionally, I found that 7,568 of 7,695 protein families common to vertebrates are also represented in the transcriptome. In order to turn the assembly from a merely collection of sequences into a valuable and useful resource for the entire research community I first annotated the sequences, predicted the open reading frames and protein domains and additionally put together multiple bits of information available for each sequence including but not limited to time-course and tissue- specific expression data and in situ hybridization results. The assembly was thereafter made available for the entire axolotl research community through a web portal I developed. Not only does the web portal provide access to the transcriptome data, it is also equipped with an engine for automated data retrieval, which could facilitate automated cross-species bioinformatics analyses. The study crossed the boundary between pure bioinformatics and biology as the transcriptome allowed for computational comparison of the axolotl and the newt in order to identify salamander-specific genes possibly implicated in regeneration and subsequent functional analysis thereof in the lab. Since regeneration closely resembles embryonic development in terms of genes involved in both processes, I first identified approximately 200 homologous contigs in axolotl and newt, which had a predicted open reading frame, but did not have homologs in non-regenerating species. The expression profile of one of those candidate genes suggested that it had a role in regeneration. I studied the molecular function of that gene using CRISPR/Cas system to confirm that it was protein-coding and to create knock-out animals to study the effect of gene knock-down and knock-out. Knock-out animals exhibited significant delays in both, limb development and tail regeneration. The exact mechanism causing this delay is currently being investigated.
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10

SAGGESE, IGOR. "NGS data analysis approaches for clinical applications." Doctoral thesis, Università del Piemonte Orientale, 2017. http://hdl.handle.net/11579/86924.

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Books on the topic "NGS"

1

Sablok, Gaurav, Sunil Kumar, Saneyoshi Ueno, Jimmy Kuo, and Claudio Varotto, eds. Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-17157-9.

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R, Floyd M., and International Conference on CANDU Fuel (3rd : 1992 : Pembroke, Ont.), eds. Behaviour of Bruce NGS-A fuel irradiated to a burnup of ̃ 500 MWh/kgU. Chalk River, Ont: Fuel Materials Branch, Chalk River Laboratories, 1992.

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Kippenberger, Martin. Martin Kippenberger: The happy end of Franz Kafka's 'Amerika' : afb. = [Abb.] = [fig.] : Sollicitatiegesprekken = [Einstell u ngs gesprärche] = job interviews. Rotterdam: Museum Boymans-van Beuningen, 1994.

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Stern, Hal. NFS und NIS: Managing von UNIX-Netzwerken. Bonn: Addison-Wesley, 1993.

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R, Floyd M., Chalk River Laboratories. Fuel Materials Branch., Atomic Energy of Canada Limited., and International Conference on CANDU Fuel (3rd : 1992 : Pembroke, Ont.), eds. An overview of the examination of fuel as follow-up to the 1988 November overpower transient in Pickering NGS-A unit 1. Chalk River, Ont: Chalk River Laboratories, 1992.

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National Genealogical Society. Conference in the States. Pennsylvania, cradle of a nation: NGS, GSP, 7-10 May, 1997, Valley Forge : National Genealogical Society, 1997 Conference in the States : program syllabus. Arlington, VA: The Society, 1997.

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Sentā, Kokusai Kyōryoku NGO. NGO dēta bukku, 2011: Sūji de miru Nihon no NGO = Data book on Japanese NGOs, 2011. [Tokyo]: Gaimushō Kokusai Kyōryokukyoku Minkan Enjo Renkeishitsu Gaimushō Shusai Heisei 22-nendo NGO ni Yoru Tēma Betsu Nōryoku Kōjō Puroguramu "NGO no Soshiki, Katsudō ni Kakawaru Dēta Bukku Sakusei", 2011.

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Sentā, Kokusai Kyōryoku NGO. NGO dēta bukku, 2011: Sūji de miru Nihon no NGO = Data book on Japanese NGOs, 2011. [Tokyo]: Gaimushō Kokusai Kyōryokukyoku Minkan Enjo Renkeishitsu Gaimushō Shusai Heisei 22-nendo NGO ni Yoru Tēma Betsu Nōryoku Kōjō Puroguramu "NGO no Soshiki, Katsudō ni Kakawaru Dēta Bukku Sakusei", 2011.

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Yŏng-jin, Sŏ, ed. UN, NGO, kŭllobŏl simin sahoe: United Nations, NGOs, and global civil society. Sŏul Tʻŭkpyŏlsi: Hanyang Taehakkyo Chʻulpʻanbu, 2000.

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Shah, Gulzar H. The role of NGOs in community health in Pakistan: A NGO pulse report. Lahore: Lahore University of Management Sciences, 2005.

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Book chapters on the topic "NGS"

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Kappelmann-Fenzl, Melanie. "NGS Data." In Next Generation Sequencing and Data Analysis, 79–104. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-62490-3_7.

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Eisele, Marius, and Melanie Kappelmann-Fenzl. "NGS Technologies." In Next Generation Sequencing and Data Analysis, 47–58. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-62490-3_4.

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Arnemann, J. "Next-Generation-Sequencing (NGS)." In Springer Reference Medizin, 1746–47. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_3542.

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Teng, Shaolei. "NGS for Sequence Variants." In Advances in Experimental Medicine and Biology, 1–20. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-1503-8_1.

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Arnemann, J. "Next-Generation-Sequencing (NGS)." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_3542-1.

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Wang, Min. "Next-Generation Sequencing (NGS)." In Clinical Molecular Diagnostics, 305–27. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-1037-0_23.

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Kappelmann-Fenzl, Melanie. "Library Construction for NGS." In Next Generation Sequencing and Data Analysis, 39–45. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-62490-3_3.

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Benoit, Gaetan, Claire Lemaitre, Guillaume Rizk, Erwan Drezen, and Dominique Lavenier. "De Novo NGS Data Compression." In Algorithms for Next-Generation Sequencing Data, 91–115. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-59826-0_4.

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Wang, Xinkun. "Next-Generation Sequencing (NGS) Technologies." In Next-Generation Sequencing Data Analysis, 57–79. 2nd ed. New York: CRC Press, 2023. http://dx.doi.org/10.1201/9780429329180-6.

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Mukhopadhyay, Chandra Sekhar, and Bhawanpreet Kaur. "NGS-Based Biomarkers in Livestock." In Livestock Diseases and Management, 107–48. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-2209-3_7.

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Conference papers on the topic "NGS"

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Yao, Jian, Rick Bennett, and Andria Bilich. "GPS Clock Products for NGS." In 56th Annual Precise Time and Time Interval Systems and Applications Meeting, 49–53. Institute of Navigation, 2025. https://doi.org/10.33012/2025.19951.

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LaSalle, Anita. "Workshop 10 introduction: The Next Generation Software (NGS) workshop - NGS 2008." In Distributed Processing Symposium (IPDPS). IEEE, 2008. http://dx.doi.org/10.1109/ipdps.2008.4536095.

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Lamas, Gustavo Manginelli, Yves Glauber Silva dos Santos, and Marcondes Cavalcante França Jr. "Diagnostic yield of a NGS panel in Brazilian patients with sporadic peripheral neuropathy." In XIV Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s1.327.

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Introduction: The etiology of sporadic peripheral neuropathy (PN) remains unclear in a significant proportion of patients. In European and North American surveys, genetic causes have been increasingly recognized in these patients. Little is known about Latin American populations. Objective: We assessed the diagnostic yield of a comprehensive next generation sequencing (NGS) panel in a cohort of Brazilian patients with PN without family history. Potential predictors of genetic etiology in this group were further explored. Methods: We evaluated 41 consecutive adult patients regularly followed with PN and 1. Negative family history of PN or other neurological disorders and 2. Unclear etiology despite extensive laboratorial/neurophysiological (Nerve Conduction Study+Electromyography [NCS+EMG]) workup. All patients then underwent genetic testing using a comprehensive (NGS) panel that included 72 genes known to cause PN. Only pathogenic or likely pathogenic variants according to American College of Medical Genetics and Genomics criteria were retrieved. For each subject, we recorded demographic, clinical and NCS+EMG data. Such variables were then compared between positive vs negative NGS subgroups using Fisher exact test (P < 0.05). Results: Fifteen patients had diagnostic NGS results (8 men, median age = 43 years old), whereas 26 patients (15 men, median age = 47 years old) had negative or unconclusive results. Diagnostic yield of the PN panel was 15/41 = 36.5%. Seventeen distinct variants were found in 5 different genes; PMP22 and SH3TC2 were the most frequently identified. Earlier age of PN onset (P = 0.013), demyelinating pattern on NCS+EMG (P = 0.021) and presence of distal atrophy on clinical examination (P= 0.043) were associated with positive NGS results. Conclusion: A significant proportion of Brazilian patients with sporadic PN has genetic etiology. NGS emerges as a diagnostically useful tool for idiopathic PN, particularly when there is earlier age at onset and demyelinating features.
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La Penna, Paolo. "ELT Pre-Focal Station NGS Sensor Arm." In Adaptive Optics for Extremely Large Telescopes 5. Instituto de Astrofísica de Canarias (IAC), 2017. http://dx.doi.org/10.26698/ao4elt5.0018.

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Kchouk, Mehdi, Jean-Francois Gibrat, and Mourad Elloumi. "An Error Correction Algorithm for NGS Data." In 2017 28th International Workshop on Database and Expert Systems Applications (DEXA). IEEE, 2017. http://dx.doi.org/10.1109/dexa.2017.33.

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Atashpaz-Gargari, Esmaeil, Mohammad Shahrokh Esfahani, Richard Metz, and Charles D. Johnson. "OBGene: Optimal barcode generator for NGS experiments." In 2012 IEEE International Workshop on Genomic Signal Processing and Statistics (GENSIPS). IEEE, 2012. http://dx.doi.org/10.1109/gensips.2012.6507734.

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Riccardi, A., J. Antichi, F. Quirós-Pacheco, S. Esposito, L. Carbonaro, G. Agapito, V. Biliotti, et al. "The NGS Pyramid wavefront sensor for ERIS." In SPIE Astronomical Telescopes + Instrumentation, edited by Enrico Marchetti, Laird M. Close, and Jean-Pierre Véran. SPIE, 2014. http://dx.doi.org/10.1117/12.2057920.

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Honisch, E., R. Fröhlich, AS Vesper, I. Beyer, T. Fehm, and D. Niederacher. "NGS-basierte Multigenpanel-Analyse BRCA1-assoziierter TNBCs." In 39. Jahrestagung der Deutschen Gesellschaft für Senologie. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1687985.

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Budati, Krishnaveni, Jinoh Kim, Abhishek Chandra, and Jon Weissman. "NGS: Service Adaptation in Open Grid Platforms." In 2007 IEEE International Parallel and Distributed Processing Symposium. IEEE, 2007. http://dx.doi.org/10.1109/ipdps.2007.370504.

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Esposito, S., P.-M. Gori, and G. Brusa. "Single NGS for Extremely Large Telescope MCAO." In 1st AO4ELT conference - Adaptive Optics for Extremely Large Telescopes. Les Ulis, France: EDP Sciences, 2010. http://dx.doi.org/10.1051/ao4elt/201004003.

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Reports on the topic "NGS"

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Cieśla, Marek. NGS Bladder cancer. University of Rzeszow, 2024. http://dx.doi.org/10.15584/ngs.rd.2024.

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The study group comprises 46 patients with urothelial bladder cancers referred for transurethral resection of the tumour. A molecular study on tumour-derived DNA was performed using next-generation sequencing. Somatic mutations were screened in 50 genes involved in cancerogenesis
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Vuyisich, Momchilo. Next generation sequencing (NGS)technologies and applications. Office of Scientific and Technical Information (OSTI), September 2012. http://dx.doi.org/10.2172/1050518.

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Fondeur, Fernando, Kathryn Taylor-Pashow, Daniel Jones, and Thomas Peters. Analysis and Testing of Parsons NGS Solvent Formulation. Office of Scientific and Technical Information (OSTI), April 2021. http://dx.doi.org/10.2172/1784922.

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Peters, T. SAMPLE RESULTS FROM ROUTINE SAMPLES AFTER THE NGS CHANGEOVER. Office of Scientific and Technical Information (OSTI), December 2014. http://dx.doi.org/10.2172/1165535.

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Peters, T., and M. Williams. RESULTS OF ANALYSIS OF NGS CONCENTRATE DRUM SAMPLES [Next Generation Solvent]. Office of Scientific and Technical Information (OSTI), September 2013. http://dx.doi.org/10.2172/1093873.

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Moyer, Bruce A., Joseph F. Birdwell, Jr, Peter V. Bonnesen, Stephanie H. Bruffey, Laetitia Helene Delmau, Nathan C. Duncan, Dale Ensor, et al. Next Generation Solvent (NGS): Development for Caustic-Side Solvent Extraction of Cesium. Office of Scientific and Technical Information (OSTI), March 2014. http://dx.doi.org/10.2172/1237596.

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Agarwal, Rahul. NGS based molecular characterization of a region affecting 5alphaandrost-16-en-3-one. Cold Spring Harbor Laboratory, October 2016. http://dx.doi.org/10.1101/079855.

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งามประเสริฐวงศ์, ธงชัย, อาจอง ประทัตสุนทรสาร, and พชรพล จุ่มศรี. สถานภาพและการใช้ทรัพยากรของค้างคาวคุณกิตติ Craseonycteris thonglongyai : รายงานผลการดำเนินงาน. ภาควิชาชีววิทยา คณะวิทยาศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2019. https://doi.org/10.58837/chula.res.2019.28.

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ค้างคาวกินแมลงมีบทบาทสำคัญต่อระบบนิเวศในการควบคุมประชากรแมลงในธรรมชาติ ในงานวิจัยนี้ได้ทำการศึกษาองค์ประกอบอาหารของค้างคาวคุณกิตติจากมูลค้างคาวที่เก็บจากถ้ำพระ ในพื้นที่โครงการอนุรักษ์พันธุกรรมพืชอันเนื่องมาจากพระราชดำริฯ อำเภอไทรโยค จังหวัดกาญจนบุรี โดยใช้ดีเอ็นเอบาร์โค้ดในการระบุชนิดของแมลง และใช้ไพร์เมอร์ที่มีความจำเพาะต่อแมลง (ZBJ-ArtF1c และ ZBJ-ArtR2c) ในปฏิกิริยาลูกโซ่โพลีเมอเรส (PCR) เพื่อเพิ่มจำนวนดีเอ็นเอของยีน cytochrome c oxidase subunit I (COI) ผลจากการวิเคราะห์ลำดับดีเอ็นเอด้วยเทคนิค next-generation sequencing (NGS) พบว่า อาหารของค้างคาวคุณกิตติส่วนใหญ่เป็นแมลงในอันดับผีเสื้อ (Lepidoptera) ในวงศ์ Noctuidae, Hyblaeidae, Geometridae, Depressariidae และ Oecophori นอกจากนั้นยังประกอบด้วยแมลงจำพวกจิ้งหรีด (อันดับ Orthoptera วงศ์ Gryllidae) และมวนง่าม (อันดับ Hemiptera วงศ์ Pentatomidae) ซึ่งพบว่าแมลงบางชนิดนั้นจัดว่าเป็นแมลงศัตรูพืชที่สำคัญ เช่น ผีเสื้อกินใบสัก Hyblaea puera ดังนั้นค้างคาวคุณกิตติแมลงจึงมีบทบาทสำคัญในการควบคุมแมลงศัตรูพืชในธรรมชาติอีกด้วย
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Sloop Jr, Frederick {Fred} V. A GC-FID-Based Method for Quantifying the Breakdown of TiDG in NGS Solvent. Office of Scientific and Technical Information (OSTI), September 2018. http://dx.doi.org/10.2172/1491325.

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Washington, A. L. II, and T. B. Peters. Sample Results From The Extraction, Scrub, And Strip Test For The Blended NGS Solvent. Office of Scientific and Technical Information (OSTI), March 2014. http://dx.doi.org/10.2172/1123835.

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