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Relevant bibliographies by topics / Nickase

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Academic literature on the topic 'Nickase'

Author: Grafiati

Published: 4 June 2021

Last updated: 27 April 2022

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Journal articles on the topic "Nickase"

1

He, Yan, and Tao Jiang. "Nickase-dependent isothermal DNA amplification." Advances in Bioscience and Biotechnology 04, no. 04 (2013): 539–42. http://dx.doi.org/10.4236/abb.2013.44070.

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Wu, Haibo, Yongsheng Wang, Yan Zhang, Mingqi Yang, Jiaxing Lv, Jun Liu, and Yong Zhang. "TALE nickase-mediatedSP110knockin endows cattle with increased resistance to tuberculosis." Proceedings of the National Academy of Sciences 112, no. 13 (March 2, 2015): E1530—E1539. http://dx.doi.org/10.1073/pnas.1421587112.

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Transcription activator-like effector nuclease (TALEN)-mediated genome modification has been applied successfully to create transgenic animals in various species, such as mouse, pig, and even monkey. However, transgenic cattle with gene knockin have yet to be created using TALENs. Here, we report site-specific knockin of the transcription activator-like effector (TALE) nickase-mediated SP110 nuclear body protein gene (SP110) via homologous recombination to produce tuberculosis-resistant cattle. In vitro and in vivo challenge and transmission experiments proved that the transgenic cattle are able to control the growth and multiplication ofMycobacterium bovis, turn on the apoptotic pathway of cell death instead of necrosis after infection, and efficiently resist the low dose ofM.bovistransmitted from tuberculous cattle in nature. In this study, we developed TALE nickases to modify the genome of Holstein–Friesian cattle, thereby engineering a heritable genome modification that facilitates resistance to tuberculosis.

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Kim, Do Yon, Su Bin Moon, Jeong-Heon Ko, Yong-Sam Kim, and Daesik Kim. "Unbiased investigation of specificities of prime editing systems in human cells." Nucleic Acids Research 48, no. 18 (September 17, 2020): 10576–89. http://dx.doi.org/10.1093/nar/gkaa764.

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Abstract Prime editors (PEs) enable targeted precise editing, including the generation of substitutions, insertions and deletions, in eukaryotic genomes. However, their genome-wide specificity has not been explored. Here, we developed Nickase-based Digenome-seq (nDigenome-seq), an in vitro assay that uses whole-genome sequencing to identify single-strand breaks induced by CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 (CRISPR-associated protein 9) nickase. We used nDigenome-seq to screen for potential genome-wide off-target sites of Cas9 H840A nickase, a PE component, targeted to nine human genomic sites. Then, using targeted amplicon sequencing of off-target candidates identified by nDigenome-seq, we showed that only five off-target sites showed detectable PE-induced modifications in cells, at frequencies ranging from 0.1 to 1.9%, suggesting that PEs provide a highly specific method of precise genome editing. We also found that PE specificity in human cells could be further improved by incorporating mutations from engineered Cas9 variants, particularly eSpCas9 and Sniper Cas9, into PE.

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Jansson, Vuokko, and Kristian Jansson. "A bioluminescent DNA nickase assay of deoxyribonuclease I." Analytical Biochemistry 333, no. 2 (October 2004): 402–4. http://dx.doi.org/10.1016/j.ab.2004.05.060.

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Yunusova, A. K., E. A. Rogulin, R. I. Artyukh, L. A. Zheleznaya, and N. I. Matvienko. "Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex." Biochemistry (Moscow) 71, no. 7 (July 2006): 815–20. http://dx.doi.org/10.1134/s0006297906070157.

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Davis, Luther, and Nancy Maizels. "Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair." Proceedings of the National Academy of Sciences 111, no. 10 (February 20, 2014): E924—E932. http://dx.doi.org/10.1073/pnas.1400236111.

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DNA nicks are the most common form of DNA damage, and if unrepaired can give rise to genomic instability. In human cells, nicks are efficiently repaired via the single-strand break repair pathway, but relatively little is known about the fate of nicks not processed by that pathway. Here we show that homology-directed repair (HDR) at nicks occurs via a mechanism distinct from HDR at double-strand breaks (DSBs). HDR at nicks, but not DSBs, is associated with transcription and is eightfold more efficient at a nick on the transcribed strand than at a nick on the nontranscribed strand. HDR at nicks can proceed by a pathway dependent upon canonical HDR factors RAD51 and BRCA2; or by an efficient alternative pathway that uses either ssDNA or nicked dsDNA donors and that is strongly inhibited by RAD51 and BRCA2. Nicks generated by either I-AniI or the CRISPR/Cas9D10A nickase are repaired by the alternative HDR pathway with little accompanying mutagenic end-joining, so this pathway may be usefully applied to genome engineering. These results suggest that alternative HDR at nicks may be stimulated in physiological contexts in which canonical RAD51/BRCA2-dependent HDR is compromised or down-regulated, which occurs frequently in tumors.

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Ren, Xingjie, Zhihao Yang, Decai Mao, Zai Chang, Huan-Huan Qiao, Xia Wang, Jin Sun, et al. "Performance of the Cas9 Nickase System in Drosophila melanogaster." G3: Genes|Genomes|Genetics 4, no. 10 (August 15, 2014): 1955–62. http://dx.doi.org/10.1534/g3.114.013821.

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Christensen, Jesper, Susan F. Cotmore, and Peter Tattersall. "Minute Virus of Mice Initiator Protein NS1 and a Host KDWK Family Transcription Factor Must Form a Precise Ternary Complex with Origin DNA for Nicking To Occur." Journal of Virology 75, no. 15 (August 1, 2001): 7009–17. http://dx.doi.org/10.1128/jvi.75.15.7009-7017.2001.

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ABSTRACT Parvoviral rolling hairpin replication generates palindromic genomic concatemers whose junctions are resolved to give unit-length genomes by a process involving DNA replication initiated at origins derived from each viral telomere. The left-end origin of minute virus of mice (MVM), oriL, contains binding sites for the viral initiator nickase, NS1, and parvovirus initiation factor (PIF), a member of the emerging KDWK family of transcription factors. oriL is generated as an active form, oriLTC, and as an inactive form, oriLGAA, which contains a single additional nucleotide inserted between the NS1 and PIF sites. Here we examined the interactions on oriLTC which lead to activation of NS1 by PIF. The two subunits of PIF, p79 and p96, cooperatively bind two ACGT half-sites, which can be flexibly spaced. When coexpressed from recombinant baculoviruses, the PIF subunits preferentially form heterodimers which, in the presence of ATP, show cooperative binding with NS1 on oriL, but this interaction is preferentially enhanced on oriLTC compared to oriLGAA. Without ATP, NS1 is unable to bind stably to its cognate site, but PIF facilitates this interaction, rendering the NS1 binding site, but not the nick site, resistant to DNase I. Varying the spacing of the PIF half-sites shows that the distance between the NS1 binding site and the NS1-proximal half-site is critical for nickase activation, whereas the position of the distal half-site is unimportant. When expressed separately, both PIF subunits form homodimers that bind site specifically to oriL, but only complexes containing p79 activate the NS1 nickase function.

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Nüesch, Jürg P. F., Jesper Christensen, and Jean Rommelaere. "Initiation of Minute Virus of Mice DNA Replication Is Regulated at the Level of Origin Unwinding by Atypical Protein Kinase C Phosphorylation of NS1." Journal of Virology 75, no. 13 (July 1, 2001): 5730–39. http://dx.doi.org/10.1128/jvi.75.13.5730-5739.2001.

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ABSTRACT Minute virus of mice nonstructural protein NS1 is a multifunctional protein that is involved in many processes necessary for virus propagation. To perform its distinct activities in timely coordinated manner, NS1 was suggested to be regulated by posttranslational modifications, in particular phosphorylation. In fact, NS1 replicative functions are dependent on protein kinase C (PKC) phosphorylation, most likely due to alteration of the biochemical profile of the viral product as determined by comparing native NS1 with its dephosphorylated counterpart. Through the characterization of NS1 mutants at individual PKC consensus phosphorylation sites for their biochemical activities and nickase function, we were able to identify two target atypical PKC phosphorylation sites, T435 and S473, serving as regulatory elements for the initiation of viral DNA replication. Furthermore, by dissociating the energy-dependent helicase activity from the ATPase-independent trans esterification reaction using partially single-stranded substrates, we could demonstrate that atypical PKC regulation of NS1 nickase activity occurs at the level of origin unwinding prior to trans esterification.

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Liebendorfer, Adam. "Novel Cas9-nickase technique repairs mutation without off-target modifications." Scilight 2018, no. 49 (December 3, 2018): 490001. http://dx.doi.org/10.1063/1.5082920.

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Dissertations / Theses on the topic "Nickase"

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Schiml, Simon [Verfasser], and H. [Akademischer Betreuer] Puchta. "Untersuchungen zur Gentechnologie und DNA-Reparatur in Pflanzen mithilfe der Cas9 Nickase / Simon Schiml. Betreuer: H. Puchta." Karlsruhe : KIT-Bibliothek, 2016. http://d-nb.info/1110969546/34.

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Satomura, Atsushi. "Studies on the adaptational strategies to the heat stress in Saccharomyces cerevisiae and the reconstruction of thermotolerance." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225664.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第20439号
農博第2224号
新制||農||1049(附属図書館)
学位論文||H29||N5060(農学部図書室)
京都大学大学院農学研究科応用生命科学専攻
(主査)教授植田充美, 教授間藤徹, 教授喜多恵子
学位規則第4条第1項該当

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Ramirez, Cherie Lynn. "Improving Zinc Finger Nucleases - Strategies for Increasing Gene Editing Activities and Evaluating Off-Target Effects." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10319.

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Zinc finger nucleases (ZFNs) induce double-strand DNA breaks at specific recognition sites. ZFNs can dramatically increase the efficiency of incorporating desired insertions, deletions, or substitutions in living cells. These tools have revolutionized the field of genome engineering in several model organisms and cell types including zebrafish, rats, and human pluripotent stem cells. There have been numerous advances in ZFN engineering and characterization strategies, some of which are detailed in this work. The central theme of this dissertation is improving the activity and specificity of engineered zinc finger nucleases with the ultimate goal of increasing the safety and efficacy of these tools for human therapy. As a first step, I undertook a large-scale effort to demonstrate that the modular assembly method of ZFN synthesis has a significantly higher failure rate than previously reported in the literature. This strongly suggested that engineering of ZFNs should better account for context-dependent effects among zinc fingers. The second advance reported in this dissertation is a method for biasing repair of zinc finger protein-induced DNA breaks toward homology-driven rather than error-prone repair in the presence of a donor template. Catalytically inactivating one monomer of a ZFN dimer results in a zinc finger nickase (ZFNickase) whose cleavage preference is directed at only one DNA strand. In human cell reporter assays, these ZFNickases exhibit a higher likelihood of repair by homology-driven processes, albeit with reduced absolute rates of correction. With further optimization, zinc finger nickases could provide a safer alternative to ZFNs in the context of gene correction therapies. Third, realizing there was no robust method for determining off-target cleavage sites of ZFNs in a genome-wide manner, I validated a collaborator’s novel in vitro selection system in human cells by identifying eight new potential off-target cleavage sites for a ZFN pair currently being used in clinical trials. Although it is unlikely these low-frequency mutations would be deleterious to patients, these results demonstrated that ZFNs induced more off-target effects than had been appreciated by previous work in the field. Collectively, the findings of this dissertation have contributed to more robust strategies for designing and evaluating ZFNs.

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Gabsalilow, Lilia [Verfasser]. "Entwicklung hochspezifischer Nickasen für gene targeting-Anwendungen / Lilia Gabsalilow." Gießen : Universitätsbibliothek, 2014. http://d-nb.info/1068773006/34.

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Al-Khyatt, Ala'a Ismael Mohammed. "High temperature chloride induced corrosion of nickle and nickle based alloys." Thesis, Cranfield University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305267.

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Robbertse, Nicolaas Johannes. "Hersiening van KVBA-beslissings / deur Nickus Robbertse." Thesis, North-West University, 2010. http://hdl.handle.net/10394/4239.

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Nickles, Hannah Theresa [Verfasser]. "Quantitative Analyse des Totraums unter mechanischer Beatmung / Hannah Theresa Nickles." Berlin : Freie Universität Berlin, 2015. http://d-nb.info/107747833X/34.

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Nicklas, Jan-Peter Georg [Verfasser]. "Ansatz für ein modellbasiertes Anforderungsmanagement für Unternehmensnetzwerke / Jan-Peter Georg Nicklas." Aachen : Shaker, 2016. http://d-nb.info/1104047926/34.

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Nicklas, Andrew Bliss. "Ah!" Thesis, Montana State University, 2009. http://etd.lib.montana.edu/etd/2009/nicklas/NicklasA0509.pdf.

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Ah! This show is a physical explanation of discovery - an attempt to better understand the role of contemporary hand thrown pottery and what it means to make it. A hand thrown pot can contain nourishment as well as it can provide it. Pottery can infuse artful practice and a direct relationship to the producer into everyday objects. It is the potter's responsibility to continue to push form and surface to make the best pots possible. Well crafted handmade objects support the argument for locality and autonomy, which give them greater value in a contemporary context rooted in ambiguous commodity consumption. It is our responsibility to craft the things we consume.

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Nicklas, Jörg [Verfasser]. "Vergleich nationaler und internationaler Standards der Unternehmensbewertung : Ein kontingenztheoretischer Ansatz / Jörg Nicklas." Aachen : Shaker, 2008. http://d-nb.info/1162792965/34.

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Books on the topic "Nickase"

1

Jennings, Sharon. Franklin's nickname. New York: Scholastic Inc. with arrangement with Kids Can Press Ltd., 2004.

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Rose, Maddy. A nickname. [London]: Red Fox, 2007.

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Anastasio, Dina. The best nickname. New York: Golden Book, 1986.

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Philip, Webb, ed. Nick's glasses. Wellington, New Zealand: Learning Media, 1997.

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Nick's trip. New York: Back Bay Books, 2011.

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Blatchford, Claire H. Nick's mission. Minneapolis: Lerner Publications, 1995.

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Campbell, Robert. Plugged nickle. New York: Pocket Books, 1988.

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John, Harvey. Nick's blues. Rearsby: W F Howes, 2009.

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John, Harvey. Nick's blues. Paris: Syros-Jeunesse, 2005.

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Nick's trip. London: Serpent's Tail, 1998.

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Book chapters on the topic "Nickase"

1

Kuroda, Kouichi, and Mitsuyoshi Ueda. "CRISPR Nickase-Mediated Base Editing in Yeast." In Methods in Molecular Biology, 27–37. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0868-5_3.

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Koletzko, B., K. Dokoupil, and U. v. Schenck. "Nicks Lieblingsrezepte." In Hast Du auch hohes Cholesterin?, 51–56. Heidelberg: Steinkopff, 1996. http://dx.doi.org/10.1007/978-3-642-61213-8_6.

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Canossa, Alessandro. "Interview with Nicklas “Nifflas” Nygren." In Game Analytics, 471–73. London: Springer London, 2013. http://dx.doi.org/10.1007/978-1-4471-4769-5_20.

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Wang, Cheng, Wenyuan Yu, Wenxin Li, and Zhuoqun Xu. "A Novel Method of Automobiles’ Chinese Nickname Recognition." In Computer Processing of Oriental Languages. Language Technology for the Knowledge-based Economy, 68–78. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-00831-3_7.

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Pardasani, R. T., and P. Pardasani. "Magnetic properties of nickle(II) phthalocyanine polymer." In Magnetic Properties of Paramagnetic Compounds, Magnetic Susceptibility Data, Volume 3, 285–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 2021. http://dx.doi.org/10.1007/978-3-662-62470-8_113.

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Mater, Nadire. "Our Nickname Among the Terrorists Was “Battalion of Bastards”." In Voices from the Front, 189–98. New York: Palgrave Macmillan US, 1998. http://dx.doi.org/10.1007/978-1-4039-8188-2_27.

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Zhao, Lilin, and Jianghua Sun. "Pinewood Nematode Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle." In Biological Invasions and Its Management in China, 3–21. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3427-5_1.

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Schiml, Simon, Friedrich Fauser, and Holger Puchta. "CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases." In Methods in Molecular Biology, 111–22. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-4931-1_8.

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Seeman, Nadrian C., Chengde Mao, Furong Liu, Ruojie Sha, Xiaoping Yang, Lisa Wenzler, Xiaojun Li, et al. "Nicks, Nodes, and New Motifs for DNA Nanotechnology." In Frontiers of Nano-Optoelectronic Systems, 177–97. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-010-0890-7_12.

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Gallori, Corinna Tania. "L’immagine del Nome di Gesù presso i gesuati." In Le vestigia dei gesuati, 227–48. Florence: Firenze University Press, 2020. http://dx.doi.org/10.36253/978-88-5518-228-7.16.

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Given that the Jesuati were thus called because of their frequent invocation of “Jesus”, it would stand to reason that they frequently displayed depictions of this name, the IHS, in their churches and settlements, as a testament to their devotion as well as a reference to their own nickname. And yet, their adoption of the Holy Name was not as straightforward as it first appears.

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Conference papers on the topic "Nickase"

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Heraty, Laura, and Vincent Dion. "C03 FAN1 prevents crispr-CAS9 nickase-induced contractions of CAG/CTG repeats." In EHDN Abstracts 2021. BMJ Publishing Group Ltd, 2021. http://dx.doi.org/10.1136/jnnp-2021-ehdn.27.

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Xiaofeng Lin and Yang Guo. "A dynamic model of single flow Zinc-Nickle battery." In 2015 Chinese Automation Congress (CAC). IEEE, 2015. http://dx.doi.org/10.1109/cac.2015.7382705.

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Bobrova, Maria V. "Modern nicknames of residents of the Perm region: problems of lexicographic description." In Lexicography of the digital age. TSU Press, 2021. http://dx.doi.org/10.17223/978-5-907442-19-1-2021-61.

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Multi-aspect problems of lexicographic description of modern nicknames of residents of the Perm region are identified: the volume, sources and methods of collecting nickname materials, the writing of nicknames, and the ethical aspect of their lexicography. The principles of description of nicknames, which are determined by them and accepted by the author in the projected dictionary, are specified.

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Oueslati, Basma, Taha Ben Salah, Chiraz Larbi Aguili, and Taoufik Aguili. "Planar antenna miniaturization by inserting nicks method." In 2014 World Congress on Computer Applications and Information Systems (WCCAIS). IEEE, 2014. http://dx.doi.org/10.1109/wccais.2014.6916619.

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Yuming, Qiu, Yu Deqin, Cao Wenjie, Xiao Tianjin, He Zhibin, Liu Wei, Jing Xubin, Fang Jingxun, and Albert Pang. "Species selection of pre-amorphous implantation in Nickle Silicide process." In 2015 China Semiconductor Technology International Conference (CSTIC). IEEE, 2015. http://dx.doi.org/10.1109/cstic.2015.7153336.

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Pearson, Aric M., Randy D. Curry, Kelli M. Noel, Sarah A. Mounter, and Kevin A. O'Connor. "High voltage breakdown analysis of nickle-zinc ferrite double-positive metamaterials." In 2015 IEEE Pulsed Power Conference (PPC). IEEE, 2015. http://dx.doi.org/10.1109/ppc.2015.7296891.

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Wenyuan Xu, Wei Long, and Ruihuan Du. "Theoretical research of methane reforming with activ-carbon dioxide over Nickle." In 2011 International Conference on Computer Science and Service System (CSSS). IEEE, 2011. http://dx.doi.org/10.1109/csss.2011.5972084.

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Sing, Deobrat, and Y. A. Sonvane. "Theoretical investigation of structural and electronic properties of ultrathin nickle nanowire." In 5TH NATIONAL CONFERENCE ON THERMOPHYSICAL PROPERTIES: (NCTP‐09). American Institute of Physics, 2016. http://dx.doi.org/10.1063/1.4945254.

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Inoue, Tomoya, Yuuki Takano, Shinsuke Miwa, and Yoichi Shinoda. "A design and implementation of nickname-based sockets for applications inside NATed network." In the 7th Asian Internet Engineering Conference. New York, New York, USA: ACM Press, 2011. http://dx.doi.org/10.1145/2089016.2089025.

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Zhaoli Liu, Tao Qin, Dan Zhao, Xiaohong Guan, and Tao Yang. "A new model for nickname detection based on network structure and similarity propagation." In 2016 IEEE Symposium on Computers and Communication (ISCC). IEEE, 2016. http://dx.doi.org/10.1109/iscc.2016.7543883.

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Reports on the topic "Nickase"

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Zhai, H., T. Senevirathne, R. Perlman, M. Zhang, and Y. Li. Transparent Interconnection of Lots of Links (TRILL): Pseudo-Nickname for Active-Active Access. RFC Editor, February 2016. http://dx.doi.org/10.17487/rfc7781.

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Zhang, M., D. Eastlake, R. Perlman, M. Cullen, and H. Zhai. Single Nickname for an Area Border RBridge in Multilevel Transparent Interconnection of Lots of Links (TRILL). RFC Editor, February 2022. http://dx.doi.org/10.17487/rfc9183.

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Health hazard evaluation report: HETA-93-0797-2624, Nicks Silica Company, Jackson, Tennessee. U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, January 1997. http://dx.doi.org/10.26616/nioshheta9307972624.

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