Relevant bibliographies by topics / NifHDK genes

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Academic literature on the topic 'NifHDK genes'

Author: Grafiati
Published: 11 December 2022
Last updated: 25 January 2023

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Journal articles on the topic "NifHDK genes"

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Minerdi, Daniela, Renato Fani, Romina Gallo, Alessandra Boarino, and Paola Bonfante. "Nitrogen Fixation Genes in an EndosymbioticBurkholderia Strain." Applied and Environmental Microbiology 67, no. 2 (February 1, 2001): 725–32. http://dx.doi.org/10.1128/aem.67.2.725-732.2001.

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ABSTRACT In this paper we report the identification and characterization of a DNA region containing putative nif genes and belonging to a Burkholderia endosymbiont of the arbuscular mycorrhizal fungus Gigaspora margarita. A genomic library of total DNA extracted from the fungal spores was also representative of the bacterial genome and was used to investigate the prokaryotic genome. Screening of the library with Azospirillum brasilense nifHDKgenes as the prokaryotic probes led to the identification of a 6,413-bp region. Analysis revealed three open reading frames encoding putative proteins with a very high degree of sequence similarity with the two subunits (NifD and NifK) of the component I and with component II (NifH) of nitrogenase from different diazotrophs. The three genes were arranged in an operon similar to that shown by most archaeal and bacterial diazotrophs. PCR experiments with primers designed on theBurkholderia nifHDK genes and Southern blot analysis demonstrate that they actually belong to the genome of the G. margarita endosymbiont. They offer, therefore, the first sequence for the nif operon described for Burkholderia. Reverse transcriptase PCR experiments with primers designed on theBurkholderia nifH and nifD genes and performed on total RNA extracted from spores demonstrate that the gene expression was limited to the germination phase. A phylogenetic analysis performed on the available nifK sequences placed the endosymbioticBurkholderia close to A. brasilense.
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Al-Saffar, F. A. K., and M. Z. S. Al-Khayyat. "Molecular and Biochemical Characterization of nifHDK Genes in Klebsiella pneumoniae." International Journal Bioautomation 25, no. 2 (June 30, 2021): 133–44. http://dx.doi.org/10.7546/ijba.2021.25.2.000721.

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Nitrogen fixation is carried by an enzyme complex called nitrogenase which consists of two main components, a dinitrogenase that is encoded by nifD and nifK and an iron containing reductase, also called Fe protein which is encoded by nifH. Nitrogen-free medium was used to detect the ability of nitrogen fixation by Klebsiella pneumonia, then DNA was extracted and overlap extension polymerase chain reaction of nifH, nifD and nifK. To obtain nucleotide sequences of these genes, sequencing of the PCR products was one. The reverse sequence of nifH and the forward sequences of nifD and nifK were converted into amino acids using online translation tool. Homology modeling was carried out using SWISS-MODEL. The modeled amino acids sequences was validated using ERRAT and PROCHECK. The modeled sequences were reliable and of quality higher than 90%. The two subunits of Fe protein were constructed and tertiary structure was predicted together with the binding sites for prosthetic group and ADP molecule in Fe protein. The following amino acids Asp11, Lys13, Asn157, Ser158, Val183, Pro184, Arg185, Asp186, Val189, Gln190 and Glu193 seem to participate in the ADP binding. The complexity of this enzyme makes it difficult to be cloned in plants.
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Kasap, Murat, and Jiann-Shin Chen. "Clostridium pasteurianum W5 synthesizes two NifH-related polypeptides under nitrogen-fixing conditions." Microbiology 151, no. 7 (July 1, 2005): 2353–62. http://dx.doi.org/10.1099/mic.0.27931-0.

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Previous studies identified five nifH-like genes (nifH2 through nifH6) in Clostridium pasteurianum (strain W5), where the nifH1 gene encodes the nitrogenase iron protein. Transcripts of these nifH genes, with the exception of nifH3, were detected in molybdenum-sufficient nitrogen-fixing cells. However, the size of the transcripts, the level of transcription and the presence of polypeptides encoded by the nifH-like genes were not reported. The nifH2 and nifH6 genes were extremely similar, as they seemed to differ by only two bases in a span of 2481 bp, one in the coding region and another in the upstream region. Re-examination of the DNA sequences revealed that the coding region of nifH2 and nifH6 was identical, whereas the difference in the upstream region was confirmed. Results from the authors' ongoing study of the nif genes of single-colony isolates of C. pasteurianum suggest that the nifH6 designation should be eliminated. Here the size of mRNA from nifH2 and the detection of the NifH2 polypeptide in nitrogen-fixing cells of C. pasteurianum are reported. Northern blot analysis of periodically collected nitrogen-fixing cells showed that the nifH1 and nifH2 mRNAs were present throughout growth. Addition of ammonium acetate repressed the transcription of both these genes similarly. Using an antiserum raised against NifH of Azotobacter vinelandii, two NifH-related bands were detected by Western blot analysis after electrophoretic separation of proteins in extracts of nitrogen-fixing C. pasteurianum cells. After separation of proteins by preparative SDS-PAGE, the NifH polypeptides were characterized by MALDI-TOF-MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) and by ES-MS/MS (electrospray tandem mass spectrometry) analyses. The results confirmed the presence of NifH2, in addition to NifH1, in nitrogen-fixing C. pasteurianum cells.
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Sibold, Lionel, Dominique Pariot, Lakshmi Bhatnagar, Marc Henriquet, and Jean-Paul Aubert. "Hybridization of DNA from methanogenic bacteria with nitrogenase structural genes (nifHDK)." Molecular and General Genetics MGG 200, no. 1 (June 1985): 40–46. http://dx.doi.org/10.1007/bf00383310.

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Ioannidis, I., and M. Buck. "Nucleotide sequence of the Klebsiella pneumoniae nifD gene and predicted amino acid sequence of the α-subunit of nitrogenase MoFe protein." Biochemical Journal 247, no. 2 (October 15, 1987): 287–91. http://dx.doi.org/10.1042/bj2470287.

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The nucleotide sequence of the Klebsiella pneumoniae nifD gene is presented and together with the accompanying paper [Holland, Zilberstein, Zamir & Sussman (1987) Biochem. J. 247, 277-285] completes the sequence of the nifHDK genes encoding the nitrogenase polypeptides. The K. pneumoniae nifD gene encodes the 483-amino acid-residue nitrogenase alpha-subunit polypeptide of Mr 54156. The alpha-subunit has five strongly conserved cysteine residues at positions 63, 89, 155, 184 and 275, some occurring in a region showing both primary sequence and potential structural homology to the K. pneumoniae nitrogenase beta-subunit. A comparison with six other alpha-subunit amino acid sequences has been made, which indicates a number of potentially important domains within alpha-subunits.
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Hirsch, A. M., H. I. McKhann, A. Reddy, J. Liao, Y. Fang, and C. R. Marshall. "Assessing horizontal transfer of nifHDK genes in eubacteria: nucleotide sequence of nifK from Frankia strain HFPCcI3." Molecular Biology and Evolution 12, no. 1 (January 1, 1995): 16–27. http://dx.doi.org/10.1093/oxfordjournals.molbev.a040184.

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Egener, Tanja, Thomas Hurek, and Barbara Reinhold-Hurek. "Use of Green Fluorescent Protein to Detect Expression of nif Genes of Azoarcus sp. BH72, a Grass-Associated Diazotroph, on Rice Roots." Molecular Plant-Microbe Interactions® 11, no. 1 (January 1998): 71–75. http://dx.doi.org/10.1094/mpmi.1998.11.1.71.

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A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in association with plant roots. The bacteria expressed nitrogenase genes (nifHDK) in the rhi-zosphere, on root tips, and in epidermal cells of rice seedlings. Green fluorescent protein fusions also visualized promoter activity of single cells in soil.
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Koirala, Amrit, and Volker S. Brözel. "Phylogeny of Nitrogenase Structural and Assembly Components Reveals New Insights into the Origin and Distribution of Nitrogen Fixation across Bacteria and Archaea." Microorganisms 9, no. 8 (August 4, 2021): 1662. http://dx.doi.org/10.3390/microorganisms9081662.

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The phylogeny of nitrogenase has only been analyzed using the structural proteins NifHDK. As nifHDKENB has been established as the minimum number of genes necessary for in silico prediction of diazotrophy, we present an updated phylogeny of diazotrophs using both structural (NifHDK) and cofactor assembly proteins (NifENB). Annotated Nif sequences were obtained from InterPro from 963 culture-derived genomes. Nif sequences were aligned individually and concatenated to form one NifHDKENB sequence. Phylogenies obtained using PhyML, FastTree, RapidNJ, and ASTRAL from individuals and concatenated protein sequences were compared and analyzed. All six genes were found across the Actinobacteria, Aquificae, Bacteroidetes, Chlorobi, Chloroflexi, Cyanobacteria, Deferribacteres, Firmicutes, Fusobacteria, Nitrospira, Proteobacteria, PVC group, and Spirochaetes, as well as the Euryarchaeota. The phylogenies of individual Nif proteins were very similar to the overall NifHDKENB phylogeny, indicating the assembly proteins have evolved together. Our higher resolution database upheld the three cluster phylogeny, but revealed undocumented horizontal gene transfers across phyla. Only 48% of the 325 genera containing all six nif genes are currently supported by biochemical evidence of diazotrophy. In addition, this work provides reference for any inter-phyla comparison of Nif sequences and a quality database of Nif proteins that can be used for identifying new Nif sequences.
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Wongdee, Jenjira, Pongpan Songwattana, Nico Nouwen, Rujirek Noisangiam, Joel Fardoux, Clémence Chaintreuil, Neung Teaumroong, Panlada Tittabutr, and Eric Giraud. "nifDK Clusters Located on the Chromosome and Megaplasmid of Bradyrhizobium sp. Strain DOA9 Contribute Differently to Nitrogenase Activity During Symbiosis and Free-Living Growth." Molecular Plant-Microbe Interactions® 29, no. 10 (October 2016): 767–73. http://dx.doi.org/10.1094/mpmi-07-16-0140-r.

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Bradyrhizobium sp. strain DOA9 contains two copies of the nifDK genes, nifDKc, located on the chromosome, and nifDKp, located on a symbiotic megaplasmid. Unlike most rhizobia, this bacterium displays nitrogenase activity under both free-living and symbiotic conditions. Transcriptional analysis using gusA reporter strains showed that both nifDK operons were highly expressed under symbiosis, whereas nifDKc was the most abundantly expressed under free-living conditions. During free-living growth, the nifDKp mutation did not affect nitrogenase activity, whereas nitrogenase activity was drastically reduced with the nifDKc mutant. This led us to suppose that nifDKc is the main contributor of nitrogenase activity in the free-living state. In contrast, during symbiosis, no effect of the nifDKc mutation was observed and the nitrogen-fixation efficiency of plants inoculated with the nifDKp mutant was reduced. This suggests that nifDKp plays the main role in nitrogenase enzyme activity during symbiosis. Together, these data suggest that Bradyrhizobium sp. strain DOA9 contains two functional copies of nifDK genes that are regulated differently and that, depending on their lifestyle, contribute differently to nitrogenase activity.
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Dobritsa, S. V., and A. Yu Tomashevskii. "Homology between structural nitrogenase genes (nifHDK) from Klebsiella pneumoniae and extrachromosomal dnas from microsymbiont vesicles of Alnus glutinosa." Biopolymers and Cell 4, no. 1 (January 20, 1988): 44–48. http://dx.doi.org/10.7124/bc.000211.

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Dissertations / Theses on the topic "NifHDK genes"

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Corbett, Melissa Kaye. "Leptospirillum: a study of the nitrogen fixing capabilities." Thesis, Curtin University, 2011. http://hdl.handle.net/20.500.11937/81.

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This project sought to determine the response of Leptospirillum species subject to conditions without soluble nitrogen. The most significant outcome of this work was the identification of the nifHDK genes in L. ferriphilum and observations of its continued ability to survive in environments without soluble nitrogen. The sustained proliferation of all three Leptospirillum species studied, combined with the structural analysis of these genes, provides evidence of functional nitrogen fixation in each of these species.
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Sevilla, Myrna Quijano, and Myrna Quijano Sevilla. "Acetobacter diazotrophicus, a nitrogen-fixing bacterial endophyte of sugarcane: Analysis of nifHDK genes, plant colonization, and growth promotion." Diss., The University of Arizona, 1999. http://hdl.handle.net/10150/284150.

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Acetobacter diazotrophicus, a nitrogen-fixing bacterial endophyte, is believed to be responsible for biological nitrogen fixation (BNF) in sugarcane. However, no monocot has yet been unequivocally shown to receive fixed N through BNF. The main question addressed in this dissertation is whether A. diazotrophicus promotes plant growth, and if so, whether growth promotion is via BNF. Another question is whether the growth benefits can be extended to other grasses. To answer these questions, the nifHDK genes encoding the protein subunits of the nitrogenase enzyme were first isolated and sequenced. Secondly, Nif⁻ mutant strains were constructed by inserting a gene cassette in nifD. The growth of sugarcane plants inoculated with A. diazotrophicus wild type and Nif⁻ mutant strains were compared in growth chamber, greenhouse, and field experiments. A. diazotrophicus was also tagged with marker genes to investigate the colonization process in sugarcane and other grasses. The effect of A. diazotrophicus on the growth of other grasses was also determined. Analysis of the A. diazotrophicus NifHDK sequence revealed features typical of proteobacterial nifHDK genes and gene products. Phylogenetic analysis established the close relationship of A. diazotrophicus with the α-proteobacteria and the β-proteobacterium, Herbaspirillum seropedicae, another sugarcane endophyte. Nif⁻ mutant strains established endophytically in sugarcane plants equally well as wild type strains. ¹⁵N₂ incorporation experiments demonstrated that wild type strains but not the Nif⁻ mutants fixed N inside sugarcane plants with decreased fixation when plants were grown in medium with fixed N. In N-deficient conditions, sugarcane inoculated with wild type strains grew better and had higher total N content than either uninoculated or plants inoculated with Nif⁻ mutants. When N was not limiting, growth enhancement was observed in plants inoculated with either wild type or the Nif⁻ mutants. These results suggest that depending on the nitrogen condition, A. diazotrophicus promotes sugarcane growth via nitrogen fixation and other growth promoting factor. The results also indicated a possible strain-cultivar specificity in growth promotion. A. diazotrophicus colonized other grasses through different entry sites but was limited in the root. Under N-deficient conditions, wild type strain but not the Nif-- mutant promoted rice seedling growth indicating the beneficial effects of A. diazotrophicus to other grasses.
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Perroud, Bertrand. "Organisation transcriptionnelle des gènes de structure nifHDK de la nitrogénase chez Azospirillum brasilense Sp7 et recherche d'autres gènes impliqués dans la fixation de l'azote." Grenoble 2 : ANRT, 1986. http://catalogue.bnf.fr/ark:/12148/cb37600362r.

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Rapley, Joanne. "Phylogenetic diversity of nifH genes in Marion Island soil." Thesis, University of the Western Cape, 2006. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1001_1223535337.

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The microbial life of sub-Antarctic islands plays a key role in the islands ecosystem, with microbial activities providing the majority of nutrients available for primary production. Knowledge of microbial diversity is still in its infancy and this is particularly true regarding the diversity of micro-organisms in the Antarctic and sub-Antarctic regions. One particularly important functional group of micro-organisms is the diazotrophs, or nitrogen-fixing bacteria and archaea. This group have not been well studied in the sub-Antarctic region, but play an important role in the nutrient cycling of the island. This thesis explored the diversity of nitrogen-fixing organisms in the soil of different ecological habitats on the sub-Antarctic Marion Island.

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Salem, Hassan Samy. "Phylogenetic Analysis of the Symbiotic Nostoc Cyanobacteria as Assessed by the Nitrogen Fixation (Nifd) Gene." Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1282030524.

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Angeloni, Stephen V. "Characterization of the nifUHD cluster and a new myoglobin-like gene from Nostoc commune UTEX 584." Diss., Virginia Tech, 1992. http://hdl.handle.net/10919/37418.

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Sequence analysis of the entire 3.5 kb HindIII genomic DNA fragment previously isolated from Nostoc commune UTEX 584 (Defrancesco and Potts 1988), determined the exact locations of the nifU, nifH, and nifD genes and identified two potential stem loop structures, a direct repeat, and an ORF that codes for a protein with a predicted amino acid sequence similar to that of myoglobin from Paramecium caudatum. The N. commune UTEX 584 myoglobin-like protein has a predicted length of 118 amino acids and molecular mass of 12,906 Da. A PCR copy of the gene (glbN) was cloned for overexpression of the protein. The recombinant protein was purified and used for spectral analysis and for the production of polyclonal antisera. Treatment of the recombinant protein with dithionite and CO resulted in spectral shifts characteristic of hemoproteins that bind oxygen. While some of the spectral characteristics are unique to the protein, in general the spectra were more like those of globins than cytochromes. Based on these characteristics and the sequence similarity to the P. caudatum mnyoglobin, we proposed the name cyanoglobin, with the gene designation glbN and the protein designation GlbN. Western analysis of GlbN expression was performed on N. commune UTEX 584 and two species of Anabaena (Anabaena sp. PCC 7120 and Anabaena variabilis). In N. commune UTEX 584 a protein with a molecular mass similar to that predicted for GlbN was detected. This protein was produced in increased amounts under the same growth conditions that resulted in increased production of nitrogenase reductase (the nifH gene product). No proteins of similar size to GlbN were detected in Anabaena sp. PCC 7120 or A. variabilis. A possible function of GlbN may be for oxygen storage, transport, or protection of the nitrogenase system. These functions as well as those of the direct repeat and the potential stem loop structures and their relationship to nitrogen fixation or other physiological processes in N. commune UTEX 584 require further analysis.
Ph. D.
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Toledo, Bethânia Figueiredo Barbosa de [UNESP]. "Identificação de estirpes de rizóbios por seqüenciamento parcial dos genes 16S rDNA e nifH." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/92670.

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Made available in DSpace on 2014-06-11T19:26:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-10-29Bitstream added on 2014-06-13T18:54:07Z : No. of bitstreams: 1 toledo_bfb_me_jabo.pdf: 494396 bytes, checksum: 73007bbe5afed3d92412924b6ebe8b81 (MD5)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A crescente demanda de alimentos causada pelo aumento populacional, aliado a alto custo de fertilizantes industrializados e impacto ambiental causado por eles leva, mundialmente, à utilização em grande escala de inoculantes de bactérias fixadoras de nitrogênio. Os inoculantes utilizados no Brasil (coleção SEMIA- IPAGRO) ainda não estão suficientemente caracterizados geneticamente. O objetivo deste trabalho foi avaliar e confrontar as seqüências parciais dos genes 16S rDNA e nifH de 26 estirpes padrões já classificadas, com 70 estirpes de rizóbios recomendadas e autorizadas para a produção de inoculantes no Brasil. Para esta finalidade, a partir das amostras de DNA extraídos destas bactérias foram realizadas reações de PCR com oligonucleotídeos iniciadores relativos à região codificadora do gene 16S rDNA e do nifH, sendo então seqüenciadas com o objetivo de detectar diferenças nucleotídicas entre as diferentes bactérias estudadas. Para a comparação dos resultados do seqüenciamento com a consulta de similaridade de nucleotídeos no BLASTn, foram geradas árvores filogenéticas através de ferramentas de bioinformática. Foi observado que a classificação taxonômica das estirpes SEMIA recomendadas para inoculação de leguminosas previamente disponível na FEPAGRO, com base em propriedades morfológicas e especificidade hospedeira, não foi confirmada em todas as estirpes pelos sequenciamentos parciais dos genes estudados. Sugerimos revisão da classificação destas estirpes. Concluímos também que a consulta de similaridade ao BLASTn pelo seqüenciamento parcial dos genes 16S rDNA e nifH é, na maioria dos casos, consistente com a classificação proposta pela construção de árvores filogenéticas destas sequências. Estas ferramentas apresentaram-se muito confiáveis para obtenção de classificação em nível de gênero das estirpes estudadas.
The growing demand for food caused by population growth, combined with high cost of fertilizers and industrial environmental impacts caused by them leading, worldwide, the large scale use of inoculants different nitrogen-fixing bacteria. The inoculants used in Brazil (SEMIA-IPAGRO collection) are not yet sufficiently characterized genetically. The purpose of this study was to evaluate and compare the sequences of partial 16S rDNA and nifH of 26 strains already classified, with 70 strains of rhizobia recommended and authorized for the production of inoculants in Brazil. For this purpose, from DNA samples taken from these bacteria were performed with PCR reactions with primers on the coding region of the gene 16S rDNA and nifH, and sequencing with the aim of detecting nucleotide differences between different bacteria studied. To compare the results of the consultation of similarity of sequences of nucleotides in BLASTn, phylogenetic trees were generated through bioinformatics tools. It was observed that the taxonomic classification of strains SEMIA recommended for inoculation of legumes previously available in FEPAGRO, based on morphological properties and host specificity, it wasn’t confirmed in all strains by partial sequencing of the genes studied. We suggest reviewing the classification of these strains. We concluded that the similarity of the consultation BLASTn by partial sequencing of 16S rDNA and nifH is, in most cases, consistent with the classification proposed by the construction of phylogenetic trees of these sequences. These tools were very reliable for obtaining classified in the genus level of strains studied.
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Toledo, Bethânia Figueiredo Barbosa de. "Identificação de estirpes de rizóbios por seqüenciamento parcial dos genes 16S rDNA e nifH /." Jaboticabal : [s.n.], 2008. http://hdl.handle.net/11449/92670.

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Orientadora: Eliana Gertrudes de Macedo Lemos
Banca: Maria José Valarini
Banca: Jaime Maia dos Santos
Resumo: A crescente demanda de alimentos causada pelo aumento populacional, aliado a alto custo de fertilizantes industrializados e impacto ambiental causado por eles leva, mundialmente, à utilização em grande escala de inoculantes de bactérias fixadoras de nitrogênio. Os inoculantes utilizados no Brasil (coleção SEMIA- IPAGRO) ainda não estão suficientemente caracterizados geneticamente. O objetivo deste trabalho foi avaliar e confrontar as seqüências parciais dos genes 16S rDNA e nifH de 26 estirpes padrões já classificadas, com 70 estirpes de rizóbios recomendadas e autorizadas para a produção de inoculantes no Brasil. Para esta finalidade, a partir das amostras de DNA extraídos destas bactérias foram realizadas reações de PCR com oligonucleotídeos iniciadores relativos à região codificadora do gene 16S rDNA e do nifH, sendo então seqüenciadas com o objetivo de detectar diferenças nucleotídicas entre as diferentes bactérias estudadas. Para a comparação dos resultados do seqüenciamento com a consulta de similaridade de nucleotídeos no BLASTn, foram geradas árvores filogenéticas através de ferramentas de bioinformática. Foi observado que a classificação taxonômica das estirpes SEMIA recomendadas para inoculação de leguminosas previamente disponível na FEPAGRO, com base em propriedades morfológicas e especificidade hospedeira, não foi confirmada em todas as estirpes pelos sequenciamentos parciais dos genes estudados. Sugerimos revisão da classificação destas estirpes. Concluímos também que a consulta de similaridade ao BLASTn pelo seqüenciamento parcial dos genes 16S rDNA e nifH é, na maioria dos casos, consistente com a classificação proposta pela construção de árvores filogenéticas destas sequências. Estas ferramentas apresentaram-se muito confiáveis para obtenção de classificação em nível de gênero das estirpes estudadas.
Abstract: The growing demand for food caused by population growth, combined with high cost of fertilizers and industrial environmental impacts caused by them leading, worldwide, the large scale use of inoculants different nitrogen-fixing bacteria. The inoculants used in Brazil (SEMIA-IPAGRO collection) are not yet sufficiently characterized genetically. The purpose of this study was to evaluate and compare the sequences of partial 16S rDNA and nifH of 26 strains already classified, with 70 strains of rhizobia recommended and authorized for the production of inoculants in Brazil. For this purpose, from DNA samples taken from these bacteria were performed with PCR reactions with primers on the coding region of the gene 16S rDNA and nifH, and sequencing with the aim of detecting nucleotide differences between different bacteria studied. To compare the results of the consultation of similarity of sequences of nucleotides in BLASTn, phylogenetic trees were generated through bioinformatics tools. It was observed that the taxonomic classification of strains SEMIA recommended for inoculation of legumes previously available in FEPAGRO, based on morphological properties and host specificity, it wasn't confirmed in all strains by partial sequencing of the genes studied. We suggest reviewing the classification of these strains. We concluded that the similarity of the consultation BLASTn by partial sequencing of 16S rDNA and nifH is, in most cases, consistent with the classification proposed by the construction of phylogenetic trees of these sequences. These tools were very reliable for obtaining classified in the genus level of strains studied.
Mestre
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Santos-Caton, Ingrid R. Schneegurt Mark A. "Abundance of nifH genes in urban, agricultural, and pristine prairie streams exposed to different levels of nitrogen loading." Diss., A link to full text of this thesis in SOAR, 2007. http://soar.wichita.edu/dspace/handle/10057/1118.

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Thesis (M.S.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of Biological Sciences.
"May 2007." Title from PDF title page (viewed on Dec. 4, 2007). Thesis adviser: Mark A. Schneegurt. Includes bibliographic references (leaves 63-72).
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Zhang, Lei [Verfasser], Barbara [Akademischer Betreuer] Reinhold-Hurek, and Anke [Akademischer Betreuer] Becker. "Design and application of an oligonucleotide microarray (nifH-phylochip) for nifH gene-based detection of nitrogen-fixing prokaryotes / Lei Zhang. Gutachter: Barbara Reinhold-Hurek ; Anke Becker. Betreuer: Barbara Reinhold-Hurek." Bremen : Staats- und Universitätsbibliothek Bremen, 2005. http://d-nb.info/1072302217/34.

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Book chapters on the topic "NifHDK genes"

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Klassen, G., F. O. Pedrosa, E. M. Souza, M. G. Yates, and L. U. Rigo. "Nitrogen Fixation Genes Contiguous with the nifHDK Genes of Herbaspirillum seropedicae." In Nitrogen Fixation: From Molecules to Crop Productivity, 118. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-47615-0_51.

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Schipani, C., M. Bazzicalupo, A. Bussotti, R. Fani, E. Gallori, A. Grifoni, R. Pastorelli, and M. Polsinelli. "Regulation of the nifHDK Genes Transcription in Azospirillum Brasilense." In Nitrogen Fixation, 127–32. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3486-6_24.

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de Araujo, Elza F., Arnaldo Zaha, Irene S. Schrank, and Diógenes S. Santos. "Characterization of DNA Segments Adjacent to the nifHDK Genes of Azospirillum Brasilense Sp7 by Tn5 Site-Directed Mutagenesis." In Azospirillum IV, 16–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73072-6_3.

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Milcamps, A. "In Vitro Construction of Lacz Gene Fusions with the Nifhdk Operon of Azospirillum Brasilense." In Nitrogen Fixation, 289–90. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3486-6_50.

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Somasegaran, Padma, and Heinz J. Hoben. "Using a Nonradioactively Labeled nifKDH Gene Probe to Locate Complementary Sequences of Rhizobial DNA Immobilized on Membranes." In Handbook for Rhizobia, 313–17. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4613-8375-8_38.

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Smith, B. E., W. A. Filler, R. A. Dixon, R. M. Kemp, J. C. Ng, and T. R. Hawkes. "The nifH Gene Product is Essential for the Production of the Active FeMoco of Klebsiella pneumoniae Nitrogenase." In Inorganic Nitrogen Metabolism, 198. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71890-8_39.

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Prévost, D., V. Macheret, and G. Laguerre. "Phylogenetic Comparison of Symbiotic (NodC and NifH) and 16S rRNA Genes in Strains of Rhizobium, Mesorhizobium and Bradyrhizobium Isolated from Astragalus, Oxytropis and Onobrychis Spp." In Nitrogen Fixation: From Molecules to Crop Productivity, 205. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-47615-0_107.

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Short, Steven M., and Jonathan P. Zehr. "Quantitative Analysis of nifH Genes and Transcripts from Aquatic Environments." In Methods in Enzymology, 380–94. Elsevier, 2005. http://dx.doi.org/10.1016/s0076-6879(05)97023-7.

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