Log in

Relevant bibliographies by topics / Nigrosine / Journal articles

To see the other types of publications on this topic, follow the link: Nigrosine.

Journal articles on the topic 'Nigrosine'

Author: Grafiati

Published: 4 June 2021

Last updated: 27 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Nigrosine.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Mitxelena, J., A. Aguirre, J. M. Vicente, N. Landa, J. A. Raton, and J. L. Diaz-Perez. "Positive patch tests to nigrosine." Contact Dermatitis 38, no. 3 (1998): 175. http://dx.doi.org/10.1111/j.1600-0536.1998.tb05694.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Kohn, J. "Nigrosine the forgotten protein stain." Clinica Chimica Acta 166, no. 2-3 (1987): 335–36. http://dx.doi.org/10.1016/0009-8981(87)90438-4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Gacem, Sabrina, Jaime Catalán, Iván Yánez-Ortiz, Carles Soler, and Jordi Miró. "New Sperm Morphology Analysis in Equids: Trumorph® Vs Eosin-Nigrosin Stain." Veterinary Sciences 8, no. 5 (2021): 79. http://dx.doi.org/10.3390/vetsci8050079.

Full text

Abstract:

The evaluation of the male fertility potential is based on the analysis of the basic spermatic characteristics of concentration, motility and morphology. Thus, the study of sperm morphology is a fundamental element in the seminal analysis, but its real meaning has been biased by the techniques used for its evaluation. These techniques involve dehydration phases and subsequent staining, which involves the production of artifacts. The aim of the study is to compare two methods for equid semen morphology evaluation, Trumorph® using living sperm vs. eosin-nigrosine stain. A total of 49 ejaculates

APA, Harvard, Vancouver, ISO, and other styles

4

Kumawat, Srishti, Kiran Meghwal, Sudhish Kumar, Rakshit Ameta, and Chetna Ameta. "Kinetics of sonophotocatalytic degradation of an anionic dye nigrosine with doped and undoped zinc oxide." Water Science and Technology 80, no. 8 (2019): 1466–75. http://dx.doi.org/10.2166/wst.2019.396.

Full text

Abstract:

Abstract The current research focuses on the photocatalytic, sonocatalytic and sonophotocatalytic degradation of nigrosine dye with nitrogen-doped and undoped zinc oxide powders. The sonophotocatalytic degradation of dye was found to occur at a higher rate than during photo- or sonocatalytic processes. Nitrogen-doped and undoped zinc oxide powders were synthesized by a wet chemical method. Further, scanning electron microscopy (FESEM), electron dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), UV-VIS spectroscopy (UV-VIS) and transmission electron

APA, Harvard, Vancouver, ISO, and other styles

5

Radhi, Ibtighaa Kadhim, Mouayed Abdulaali Hussein, and Zaki Naser Kadhim. "Investigation of nigrosine, alizarin, indigo and acid fuchsin removal by modification of CaO derived from eggshell with AgI: Adsorption, kinetic and photocatalytic studies." European Journal of Chemistry 10, no. 1 (2019): 64–71. http://dx.doi.org/10.5155/eurjchem.10.1.64-71.1820.

Full text

Abstract:

Successful removal of nigrosine, alizarin, indigo and acid fuchsin dyes from aqueous solutions using modified CaO nanoparticles has been investigated. The CaO was obtained from eggshells and modified with AgI. The adsorbents were characterized using X-ray diffraction, energy dispersive X-ray spectroscopy, scanning electron microscopy and transmission electron microscopy. The kinetic studies were also investigated, the results showed that the adsorption of alizarin dye follows the pseudo-first-order model, while the adsorption of the nigrosine, indigo, and acid fuchsin follow the pseudo-second-

APA, Harvard, Vancouver, ISO, and other styles

6

Bharadwaj, Leena. "An Analysis of TiO₂ Assisted Photocatalytic Degradation of Nigrosine Dye." Chemical Reports 5, no. 1 (2025): 291–97. https://doi.org/10.25082/cr.2024.01.004.

Full text

Abstract:

This study examined the photocatalytic degradation of Nigrosine Dye (25 ppm) in aqueous solution using TiO2 nanoparticles under sunlight irradiation. The optimal degradation efficiency of 70.23% was achieved at pH 6 after 4 hours of treatment. Comprehensive water quality analysis revealed significant alterations in parameters including alkalinity, turbidity, hardness, nitrate, calcium, magnesium, COD, BOD, sulfate, temperature and pH, with water hardness exceeding WHO drinking water standards. HPTLC analysis demonstrated successful dye degradation through reduced peak numbers in densitograms.

APA, Harvard, Vancouver, ISO, and other styles

7

Goodland, F. C. "Detection of albumin by nigrosine stain using standard Helena electrophoresis equipment." Clinica Chimica Acta 161, no. 3 (1986): 357. http://dx.doi.org/10.1016/0009-8981(86)90023-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Santos, Patrícia Andrade dos, Vânia Maria França Ribeiro, Augusto Luiz Faino Alves, et al. "Morphology, morphometry, and membrane integrity of epididymal spermatozoa of spotted pacas (Cuniculus paca, Linnaeus 1766)." Semina: Ciências Agrárias 41, no. 1 (2020): 181. http://dx.doi.org/10.5433/1679-0359.2020v41n1p181.

Full text

Abstract:

The objective of this study was to evaluate the morphology, morphometry, and membrane integrity of epididymal spermatozoa of spotted pacas using spermatic cells collected from the epididymal tails of five animals. The flotation method using the ACP-123® and Botusemen Special® extenders was performed, and samples were stained in Diff-Quick and eosin-nigrosine. Descriptive statistics of data were obtained and Student’s t-test was performed. The morphology of 200 Diff-Quick-stained spermatozoa showed that they had an oval head with three vesicles in the acrosomal region, a midpiece, an elongated

APA, Harvard, Vancouver, ISO, and other styles

9

Sukata, Kazuaki, Hiroshi Takeuchi, Masayuki Shimada, and Yasuyuki Agari. "Influence of the nigrosine dye on the thermal behavior of polyamide 66." Journal of Applied Polymer Science 101, no. 5 (2006): 3270–74. http://dx.doi.org/10.1002/app.23715.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Sharp, R. R., A. B. Cunningham, J. Komlos, and J. Billmayer. "Observation of thick biofilm accumulation and structure in porous media and corresponding hydrodynamic and mass transfer effects." Water Science and Technology 39, no. 7 (1999): 195–201. http://dx.doi.org/10.2166/wst.1999.0359.

Full text

Abstract:

A series of flat plate, porous media reactor studies was performed to characterize the development and structure of thick biofilms in porous media and the subsequent effects on porous media hydrodynamics and mass transport variables including average pore velocity, hydrodynamic dispersivity, and (dye tracer) breakthrough curve features. Biofilms composed of either a mucoid strain of Pseudomonas aeruginosa or a non-mucoid strain of Ps. aeruginosa were established in the reactors over a 2-3 week period. Analysis of porous media biofilms was performed using a combination of image analysis, photog

APA, Harvard, Vancouver, ISO, and other styles

11

Vinotha, S., and A. Leema Rose. "Congo Red and Nigrosine Dye Degradation using Dimethyl Dioxirane as an Oxidising Agent." Oriental Journal Of Chemistry 37, no. 5 (2021): 1221–28. http://dx.doi.org/10.13005/ojc/370529.

Full text

Abstract:

The advanced oxidation of Congo red (CR) and Nigrosine (NI) using the combined action of dimethyl dioxirane as an oxidising agent is described in this study. The effects of several parameters, such as the concentration of the oxidising agent, the initial dye concentration, and the pH, have been investigated. At room temperature, the oxidising agent dimethyl dioxirane was employed to test the degradation of CR and NI dyes. On the degradation efficiency of CR and NI, pH’s effects, oxidising agent, and initial dye concentration were examined. The absorbance of CR and NI dyes before and after degr

APA, Harvard, Vancouver, ISO, and other styles

12

Li, Zhao-Qing, Xiao-Yan Zhang, Hai-Lin Cong, Bing Yu, and Lin Guo. "Synthesis of monodisperse cross-linked P(St-MMA-SPMAP) nanoparticles encapsulating dye nigrosine." Integrated Ferroelectrics 181, no. 1 (2017): 9–13. http://dx.doi.org/10.1080/10584587.2017.1352300.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Zhang, Shuai, Xiang-Wei Zhao, Hua Xu, Rong Zhu, and Zhong-Ze Gu. "Fabrication of photonic crystals with nigrosine-doped poly(MMA-co-DVB-co-MAA) particles." Journal of Colloid and Interface Science 316, no. 1 (2007): 168–74. http://dx.doi.org/10.1016/j.jcis.2007.07.069.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Hogmire, H. W., M. J. Wimmer, V. L. Crim, R. M. Welker, E. Wentz, and R. R. Smith. "Influence of tree growth on spray chemical deposits on peach leaves." Journal of Agricultural Science 118, no. 1 (1992): 77–81. http://dx.doi.org/10.1017/s0021859600068039.

Full text

Abstract:

SUMMARYA 50–fold increase in peach tree growth over a 98-day spraying season resulted in a 64 and 96% reduction in nigrosine dye deposit in the periphery and centre of the tree, respectively. Drift from spraying of adjacent rows made a significant contribution (18–39%) to the total chemical deposit in the tree periphery in four of six applications. Drift between rows was not a factor in centre tree deposit, except for a 38% contribution in the initial application. A variable reduced application rate of methyl parathion, calculated using a regression equation, resulted in a more uniform deposit

APA, Harvard, Vancouver, ISO, and other styles

15

Yang, Seung Hun, Sang Hun Cha, Jae Ik Kim, Min Ho Lee, Sang Gu Kim, and Sung Hun Ryu. "Change of thermal properties of polyamide-6,6/glass fiber composite by the addition of nigrosine." Polymer Bulletin 74, no. 12 (2017): 5083–94. http://dx.doi.org/10.1007/s00289-017-2005-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Jaafar, Mohammed Thamer. "UV-A Activated ZnO Mediated Photocatalytic Decolorization of Nigrosine (Acid Black 2) Dye in Aqueous Solution." Journal of Geoscience and Environment Protection 05, no. 09 (2017): 138–47. http://dx.doi.org/10.4236/gep.2017.59010.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Allchin, J. P., and G. O. Evans. "A simple rapid method for the detection of rat urinary proteins by agarose electrophoresis and nigrosine staining." Laboratory Animals 20, no. 3 (1986): 202–5. http://dx.doi.org/10.1258/002367786780865566.

Full text

Abstract:

The method described is sufficiently sensitive to detect major changes in the protein excretion patterns of rat urine, and the short time required for technical procedures makes the method suitable for screening large numbers of rat urine samples. The patterns observed for normal adult male rats are similar to previously published data, and the method may also be used to identify pseudoproteinuria.

APA, Harvard, Vancouver, ISO, and other styles

18

Kabil, Doaa, Adel y. El-Bagour, Mousa Sand, and Mohamed Kelany. "Administration of pickled olive fruits treated with acid black nigrosine dye induced hepato-renal dysfunction in mice." Biological and Biomedical Journal 2, no. 1 (2024): 76–83. http://dx.doi.org/10.21608/bbj.2024.341813.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Cekic, Bogdan, Hristiyana Kanzova, Georgi Petrov, Nevena Maksimovic, Ivan Cosic, and Aleksandar Milovanovic. "Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams." Biotehnologija u stocarstvu 38, no. 1 (2022): 31–39. http://dx.doi.org/10.2298/bah2201031c.

Full text

Abstract:

The current study aimed to examine the percentage changes of viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For this purpose, five rams were examined, and two ejaculates were obtained from each ram. Ejaculates are collected by the method of artificial vagina, during the insemination campaign. All ejaculates were diluted with a 6AG extender and frozen by the Cassou?s sequin method. Sperm viability was determined by eosin and nigrosine smears, and enzyme activity was examined spectrophotometri

APA, Harvard, Vancouver, ISO, and other styles

20

Flores, J. M., R. Z. Bar-Or, N. Bluvshtein, et al. "Absorbing aerosols at high relative humidity: closure between hygroscopic growth and optical properties." Atmospheric Chemistry and Physics Discussions 12, no. 1 (2012): 1019–52. http://dx.doi.org/10.5194/acpd-12-1019-2012.

Full text

Abstract:

Abstract. The extinction coefficient and growth factor of humidified aerosols, at 80% and 90% RH, and at 532 nm and 355 nm wavelengths were measured for size-selected particles for ammonium sulfate, IHSS Pahokee peat (a lightly absorbing humic-like substance proxy), nigrosine (a black dye to model highly absorbing substances), and a mixture of AS and nigrosine. The ratio of the humidified extinction coefficients to the dry (fRHext(%RH, Dry)) was explored. The measured fRHext(%RH, Dry) was compared to theoretical calculations based on Mie theory, using the measured growth factors and assuming h

APA, Harvard, Vancouver, ISO, and other styles

21

Khaeruddin, Khaeruddin, Hermawansyah Hermawansyah, Bahri Syamsuryadi, and Junaedi Junaedi. "STUDI MORFOMETRIK DAN MORFOLOGI SPERMATOZOA ENAM RUMPUN AYAM LOKAL INDONESIA." JURNAL KAJIAN VETERINER 9, no. 3 (2021): 123–34. http://dx.doi.org/10.35508/jkv.v9i3.5171.

Full text

Abstract:

This study aims to determine the morphometric comparison and identify abnormal sperm morphology in six types of Indonesian local chickens. Sperm observed came from six types of local chickens: red jungle fowl, Sentul chicken, Gaga’ chicken, Kampung Unggul Balitnak (KUB) chicken, Arab Silver chicken, and Bangkok chicken. Semen was collected by sequencing technique, then fresh semen was blotted on glass slides with eosin-nigrosine dye. Morphometrics and morphology of sperm were observed using a light microscope with a magnification of 100x16. ImageJ software was used for sperm morphometric obser

APA, Harvard, Vancouver, ISO, and other styles

22

Moore, Richard H., Elizabeth B. Wiggins, Adam T. Ahern, et al. "Sizing response of the Ultra-High Sensitivity Aerosol Spectrometer (UHSAS) and Laser Aerosol Spectrometer (LAS) to changes in submicron aerosol composition and refractive index." Atmospheric Measurement Techniques 14, no. 6 (2021): 4517–42. http://dx.doi.org/10.5194/amt-14-4517-2021.

Full text

Abstract:

Abstract. We evaluate the sensitivity of the size calibrations of two commercially available, high-resolution optical particle sizers to changes in aerosol composition and complex refractive index (RI). The Droplet Measurement Technologies Ultra-High Sensitivity Aerosol Spectrometer (UHSAS) and the TSI, Inc. Laser Aerosol Spectrometer (LAS) are two commonly used instruments for measuring the portion of the aerosol size distribution with diameters larger than nominally 60–90 nm. Both instruments illuminate particles with a laser and relate the single-particle light scattering intensity and coun

APA, Harvard, Vancouver, ISO, and other styles

23

Liu, Zoulin, and Stephen M. J. Moysey. "The Dependence of Electrical Resistivity-Saturation Relationships on Multiphase Flow Instability." ISRN Geophysics 2012 (October 17, 2012): 1–10. http://dx.doi.org/10.5402/2012/270750.

Full text

Abstract:

We investigate the relationship between apparent electrical resistivity and water saturation during unstable multiphase flow. We conducted experiments in a thin, two-dimensional tank packed with glass beads, where Nigrosine dyed water was injected uniformly along one edge to displace mineral oil. The resulting patterns of fluid saturation in the tank were captured on video using the light transmission method, while the apparent resistivity of the tank was continuously measured. Different experiments were performed by varying the water application rate and orientation of the tank to control the

APA, Harvard, Vancouver, ISO, and other styles

24

Tonelli, Fernanda Maria Policarpo, Christopher Santos Silva, Geicielly da Costa Pinto, et al. "Synthesis of Antioxidant Nano Zero-Valent Iron Using FeCl2 and Leucaena leucocephala Leaves’ Aqueous Extract and the Nanomaterial’s Potential to Promote the Adsorption of Tartrazine and Nigrosine." International Journal of Molecular Sciences 26, no. 12 (2025): 5751. https://doi.org/10.3390/ijms26125751.

Full text

Abstract:

Synthetic dyes are commonly present in industrial wastewater and when discharged in water bodies without receiving a treatment capable of removing or destroying them, they turn into concerning water pollutants. These organic contaminants threaten living beings due to their toxicity, and some of them can even damage DNA. Consequently, in order to achieve sustainable development, it is necessary to develop eco-friendly tools that can efficiently manage this kind of pollution. In the present study the aqueous extract from the leaves of Leucaena leucocephala (an invasive plant species native to Me

APA, Harvard, Vancouver, ISO, and other styles

25

Basioura, Athina, Georgia D. Brellou, Ioannis Tsakmakidis, Ioannis Vlemmas, Constantin M. Boscos, and Nektarios Giadinis. "Varicocele in an Adult Ram: Histopathological Examination and Sperm Quality Evaluation." Veterinary Sciences 9, no. 2 (2022): 86. http://dx.doi.org/10.3390/vetsci9020086.

Full text

Abstract:

Varicocele is a common pathological condition of testis that is related to male fertility problems. A 3-year age Chios ram had an abnormally enlarged scrotal area, was excluded from reproductive duties, and was euthanized with the owners’ permission. The main pathological finding was the presence of bilateral multinodular spermatic cord enlargement with laminated vascular thrombi. Histopathological examination revealed commonly mineralized thrombi within the lumen of veins of the pampiniform plexus, inflammation and testicular degeneration. The epididymides were transported to the laboratory a

APA, Harvard, Vancouver, ISO, and other styles

26

Pessoa, G. A., A. P. Martini, J. M. Trentin, T. Minela, and M. I. B. Rubin. "Effect of plasma separation techniques and sperm selection on sperm recovery and viability of cooled pony stallion semen for 48h at 5°C." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 72, no. 6 (2020): 2017–26. http://dx.doi.org/10.1590/1678-4162-11986.

Full text

Abstract:

ABSTRACT Separation techniques of seminal plasma [centrifugation (SC) and Sperm Filter® (SF)] and sperm selection [Androcoll-E (SCA) and filtration glass wool (GW)] were used in 24 ejaculates from 6 stallions. In experiment 1, the ejaculates were allocated into control (no spin), centrifugation at 600 g x 10min, SF and GW. In experiment 2, semen was submitted to SC, SGA and filtered through GW. Following the treatments in both experiments, samples were kept chilled at 5°C to 50 x 106 sperm/ml for 48h. The variables measured on fresh and cooling semen were pH, motility, membrane viability funct

APA, Harvard, Vancouver, ISO, and other styles

27

Beran, Jan, Ondřej Šimoník, Luděk Stádník, et al. "Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 61, no. 6 (2013): 1575–81. http://dx.doi.org/10.11118/actaun201361061575.

Full text

Abstract:

The objectives of this study were to determine and evaluate the effect of bull, diluter and addition of LDL in different concentration on the percentage rate of spermatozoa survival after cold shock. In total, four bulls were collected during a period of eight weeks. A total of 8 samples of fresh semen with required quality were processed. Three extenders were used for dilution of each sample; AndroMed®, Bioxcell® and Triladyl®, each in standard and LDL enriched variants. In the case of AndroMed® and Bioxcell®, 4, 6 and 8% of LDL were simply added. In Triladyl®, 6, 8 and 10% of LDL replaced th

APA, Harvard, Vancouver, ISO, and other styles

28

Beran, Jan, Ondřej Šimoník, Radko Rajmon, et al. "Effect of LDL Addition Into Selected Bull Sperm Diluters on Resistance of Spermatozoa Against Cold Shock." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 64, no. 2 (2016): 395–99. http://dx.doi.org/10.11118/actaun201664020395.

Full text

Abstract:

The aim of work was to determine the effect of LDL cholesterol addition into selected diluters on the resistance of spermatozoa against cold shock and on their short-term survivability during cold test. The hypothesis was that the addition of LDL cholesterol will positively affects sperm resistance to cold shock and ensures a higher survivability of spermatozoa during short-term cold survival test. Four bulls of different breeds and ages, from the same sire insemination center were used. A total of eight semen collections were processed. Each ejaculate was divided into 6 portions (3 controls a

APA, Harvard, Vancouver, ISO, and other styles

29

Baharun, Abdullah, Pirda Pertiwi, Annisa Rahmi, et al. "<b>Frozen semen quality of Pasundan bulls with different individual variations</b>." Open Veterinary Journal 15, no. 1 (2025): 244. https://doi.org/10.5455/ovj.2025.v15.i1.22.

Full text

Abstract:

Background: Pasundan bulls are one of Indonesia's indigenous cattle breeds. The quality of frozen semen is essential for the success of artificial insemination (AI), as individual variations in parameters such as sperm motility, viability, and membrane integrity greatly influence reproductive outcomes. Aim: The objective of this study was to evaluate the quality of frozen semen from Pasundan bulls with different individual variations. Methods: Frozen semen from eight Pasundan bulls were obtained from the West Java Regional Artificial Insemination Center (RAIC). Sperm motility was assessed usin

APA, Harvard, Vancouver, ISO, and other styles

30

Karmakar, Ms Tithi. "Comparative Study of Semen Cryopreservation Techniques and Their Impact on Post-Thaw Sperm Quality." International Journal for Research in Applied Science and Engineering Technology 13, no. 2 (2025): 1190–98. https://doi.org/10.22214/ijraset.2025.67073.

Full text

Abstract:

Semen cryopreservation is critical for assisted reproductive technologies (ART) and fertility preservation, yet cryoinjury during freezing-thawing remains a significant challenge. This study conducted a comparative analysis of slow freezing and vitrification techniques to evaluate their impact on post-thaw sperm quality parameters. Thirty normozoospermia samples from healthy donors were processed using slow freezing (10% glycerol, controlled-rate cooling) and vitrification (15% glycerol + 0.5M sucrose, ultra-rapid cooling). Post-thaw assessments included motility (computer-assisted sperm analy

APA, Harvard, Vancouver, ISO, and other styles

31

Pardede, Berlin Pandapotan, Wiwied Sawitri, Anny Amaliya, et al. "Protamine-2 protein abundance in Ongole Grade mature spermatozoa is unrelated to semen quality and DNA fragmentation." BIO Web of Conferences 162 (2025): 00006. https://doi.org/10.1051/bioconf/202516200006.

Full text

Abstract:

This study aimed to analyze the abundance of protein protamine-2 in mature spermatozoa of Ongole Grade bulls and its relation to semen quality and DNA fragmentation. A total of 120 frozen semen straws from six Ongole Grade bulls aged four to five years were used in this study. The bulls were then divided into two groups, group A (post-thawing motility >45%) and group B (post-thawing motility >45%). The semen quality parameters used in this study included progressive motility, viability, plasma membrane integrity, sperm head defects, and DNA fragmentation. Progressive motility was analyze

APA, Harvard, Vancouver, ISO, and other styles

32

Srivastava, Jyoti Prakash. "Pathophysiologic Mechanism of Erythrocyte Morphological Alteration in Freshwater Fish Channa punctatus Under Exposure of Nigrosine Black (Acid Black-2 a Tanning Industry Dye)." International Journal of Environment, Agriculture and Biotechnology 7, no. 1 (2022): 162–67. http://dx.doi.org/10.22161/ijeab.71.19.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Kumar, Pramod, J. S. Mehta, S. K. Ravi, et al. "Protective Efficacy of Cholesterol-Loaded Cyclodextrin (CLC) Against Sperm Morphological Abnormalities in Marwari Stallions." Animal Reproduction Update 4, no. 1 (2024): 3–10. http://dx.doi.org/10.48165/aru.2023.4.1.2.

Full text

Abstract:

The present study aimed to examine the protective potential of cholesterol loaded cyclodextrin (CLC) at varying concentrations against sperm morphological abnormalities of cooled and frozen thawed semen of Marwari stallions. A total of forty eight ejaculates (six stallions and eight ejaculates per stallion) were promptly processed following semen collection and subjected to gross assessment of semen quality attributes. The assessment of sperm morphologies in fresh equine semen was conducted using Eosin and Nigrosine staining technique. The semen samples from individual Marwari stallions were e

APA, Harvard, Vancouver, ISO, and other styles

34

Nikitkina, E., A. Musidray, S. Bogdanova, and A. Krutikova. "Search for associations of bull sperm quality with <i>ESR1</i> gene polymorphism." Genetics and breeding of animals, no. 2 (August 4, 2023): 14–19. http://dx.doi.org/10.31043/2410-2733-2023-2-14-19.

Full text

Abstract:

Materials and methods. The semen of 53 bulls was collected at OJSC Nevskoe. A total of 110 bull semen samples were analyzed. Sperm quality was determined using Argus-CASA (ArgusSoft, Russia). Membrane integrity was determined by staining the samples with nigrosine-eosin dye (Diam, Russia) and a Motic BA 410 microscope. Spermatozoa respiration was determined using an Expert-001 instrument. The functional state of the energy system was assessed by the reaction of respiration to the addition of the uncoupler of respiration and phosphorylation, 2,4 dinitrophenol (2,4-DNF). DNA for genetic analysis

APA, Harvard, Vancouver, ISO, and other styles

35

Pardede, Berlin Pandapotan, Muhammad Agil, Ni Wayan Kurniani Karja, Cece Sumantri, Iman Supriatna, and Bambang Purwantara. "PRM1 Gene Expression and Its Protein Abundance in Frozen-Thawed Spermatozoa as Potential Fertility Markers in Breeding Bulls." Veterinary Sciences 9, no. 3 (2022): 111. http://dx.doi.org/10.3390/vetsci9030111.

Full text

Abstract:

Functional genes and proteins in sperm play an essential role in bulls’ reproductive processes. They are more accurate in determining bull fertility than conventional semen quality tests. Protamine-1 (PRM1) is a gene or protein crucial for packaging and protecting sperm DNA until fertilization affects normal sperm function. This study analyzes the genes and proteins potential from PRM1 as fertility markers for different breeds of bulls utilized in the artificial insemination programs, expected to be an accurate tool in interpreting bull fertility in Indonesia. This study used Limousin, Holstei

APA, Harvard, Vancouver, ISO, and other styles

36

Rodrigues, Jeane, Raquel Santos dos Santos, Jhennifer Gomes Cordeiro, et al. "Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)." Zygote 28, no. 6 (2020): 453–58. http://dx.doi.org/10.1017/s0967199420000325.

Full text

Abstract:

SummaryThe seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of g

APA, Harvard, Vancouver, ISO, and other styles

37

Carreira, Janaina Torres, Loïc Lesobre, Sylvain Boullenger, Toni Chalah, Frédéric Lacroix, and Yves Hingrat. "Assisted Reproduction Techniques to Improve Reproduction in a Non-Model Species: The Case of the Arabian Bustard (Ardeotis arabs) Conservation Breeding Program." Animals 12, no. 7 (2022): 851. http://dx.doi.org/10.3390/ani12070851.

Full text

Abstract:

Artificial reproductive technologies are highly valuable for ex situ conservation. While Arabian bustard populations are declining and extinct in some parts of the range, the International Fund for Houbara Conservation in the United Arab Emirates implemented a conservation breeding program. Since 2012, a total of 1253 eggs were laid through natural reproduction, 1090 were incubated and 379 of these were fertile (fertility rate of 34.8%), leading to the production of 251 chicks. To improve fertility and acquire crucial knowledge for other endangered large birds, artificial reproduction was impl

APA, Harvard, Vancouver, ISO, and other styles

38

Stádník, Luděk, Radko Rajmon, Jan Beran, et al. "Influence of selected factors on bovine spermatozoa cold shock resistance." Acta Veterinaria Brno 84, no. 2 (2015): 125–31. http://dx.doi.org/10.2754/avb201584020125.

Full text

Abstract:

The objectives of this study were to determine the effects of sire, extender, and addition of Low Density Lipoprotein (LDL) to extenders used on the percentage rate of spermatozoa survival after cold shock. Two groups of extenders were compared: without LDL addition (control variants) and LDL enriched (experimental variants). Three extenders were used: AndroMed®, Bioxcell®, and Triladyl®. Experimental variants included 4–8% LDL addition into the AndroMed® and Bioxcell® extenders, and 6–10% LDL addition into the Triladyl® extender. In total, 12 samples of fresh semen were collected from 4 bulls

APA, Harvard, Vancouver, ISO, and other styles

39

Ducha, Nur, Isnawati Isnawati, Fitriari Izzatunnisa Muhaimin, Sisca Desi Prastyaningtias, Fardha Ad Durun Nafis, and Rochmatul Fitri. "Sperm quality profile of Bali bull as local Indonesian cattle in liquid storage in different diluents with the addition of synthetic antioxidants." BIO Web of Conferences 162 (2025): 00012. https://doi.org/10.1051/bioconf/202516200012.

Full text

Abstract:

Bali cattle are one of Indonesia’s local cattle, which require efforts to preserve genetic material from superior males by storing sperm at low temperatures so that it does not become extinct and can be implemented for artificial insemination. The simplest storage method is liquid storage, at 4-5°C. This study aimed to examine the quality profile of Bali bull sperm during storage at 4-5°C in CEP diluent with and without synthetic antioxidants (alpha-tocopherol, glutathione) and to compare it with the diluent media usually used by Indonesian Artificial Insemination centers for freezing bovine s

APA, Harvard, Vancouver, ISO, and other styles

40

MILOVAN, JOVIČIN, PETRUJKIĆ BRANKO, JOCIĆ ALEKSANDRA, et al. "Morphological analysis of boar spermatozoa by age and breed." Contemporary Agriculture (2012) 61, no. 1-2 (2012): 84–94. https://doi.org/10.5281/zenodo.7260966.

Full text

Abstract:

The total of 56 boars from 12 farm units (3 to 7 boars per farm) were used for cytological and morphological examination of semen. Large White (LW, n = 18); Swedish Landrace (SL, n = 11), Duroc (OA, n = 12); German Landrace (NL, n = 6), crossbreeds (OST, n = 9) boars were used for examination. Sperm was stained with eosin/nigrosine in one step. According to the findings of spermatozoa with protoplasmic droplets (PPD), boars were divided into groups with ≤ 10% of the PPD and the group with > 10% of the PPD. The impact of the PPD rate to number of live born piglets per litter and correlati

APA, Harvard, Vancouver, ISO, and other styles

41

Šichtař, J., A. Nehasilová, O. Šimoník, and F. Bubeníčková. "Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions – A pilot study." Czech Journal of Animal Science 62, No. 6 (2017): 227–33. http://dx.doi.org/10.17221/76/2016-cjas.

Full text

Abstract:

The aim of the study was to evaluate the effect of two different extenders on sperm characteristic before equilibration and post-thaw in the endangered Old Kladruber stallions. Also, the response of individual stallions to the extenders used was tested. Semen was collected from six stallions every other day within one week. After centrifugation of the collected sperm-rich fraction, the supernatant was removed and sperm pellets were divided to two aliquots; these were diluted either with Gent (Minitube, Germany) or privately manufactured lactose-EDTA-egg yolk extender (Lact). Three cryopreserve

APA, Harvard, Vancouver, ISO, and other styles

42

Parera, Hermilinda, and Victor Lenda. "Evaluation of Motility, Viability and Abnormality of Boar Spermatozoa in Various Modified Extenders." JURNAL ILMIAH PETERNAKAN TERPADU 11, no. 1 (2023): 13. http://dx.doi.org/10.23960/jipt.v11i1.p13-33.

Full text

Abstract:

The purpose of this study was to conduct an assessment on the quality of pig semen spermatozoa during storage and determine which diluent material should be used and how long pig semen can be stored. Freshly ejaculated boar semen was obtained from a two-years-old Duroc male and was diluted with Beltsville Thawing Solution (BTS) and Tris Citrate Fructose (TCF) supplemented with 5% egg yolk (KT) and extract of Borassus flabellifer Linn mesocarp (EM) 0.01% and stored at 13°C for up to 4 days. An experimental study with a completely randomized design with four groups and five repeats was applied.

APA, Harvard, Vancouver, ISO, and other styles

43

Souza, Anne K., Luiz Guilherme C. Trautwein, Cristiane S. Paranzini, et al. "Effect of refrigeration at -1°C on spermatozoa quality of domestic cats." Pesquisa Veterinária Brasileira 40, no. 4 (2020): 306–14. http://dx.doi.org/10.1590/1678-5150-pvb-6517.

Full text

Abstract:

ABSTRACT: The objective of this study was to evaluate the sperm quality obtained of domestic cats by electroejaculation and recovery of the tail of the epididymis after cooling at -1°C and 4°C for 24 and 48 hours. Twenty-nine adult cats (2 to 6kg) were used. Sperm collection was performed by electroejaculation (EEJ), and after 48 hours, the cats were orchiectomized, and sperm sample was obtained from the vas deferens and epididymis tail (EPD). The samples were diluted in ACP-117® extender, and the sperm characteristics were evaluated at three different moments: when still fresh, 24 and 48 hour

APA, Harvard, Vancouver, ISO, and other styles

44

Raseona, A. M., O. A. Ajao, L. D. Nethengwe, L. R. Madzhie, T. L. Nedambale, and D. M. Barry. "163 EVALUATION OF VIABILITY OF BULL SEMEN COLLECTED BY ELECTRO-EJACULATION USING COMMERCIAL SEMEN EXTENDER AND 2 CULTURE MEDIA AT CONTROLLED ROOM TEMPERATURE." Reproduction, Fertility and Development 29, no. 1 (2017): 190. http://dx.doi.org/10.1071/rdv29n1ab163.

Full text

Abstract:

Preservation of semen is an important process to ensure that semen quality is sufficient for assisted reproductive technologies. The aim of this study was to evaluate the viability of bull semen collected by electro-ejaculation using commercial semen extender and 2 modified culture media stored at controlled RT (24°C) for 72 h. Two Nguni bulls were used for semen collection; after collection, the semen was evaluated macroscopically for volume, pH, and colour, and microscopically for sperm motility, viability, and morphology. Uncontaminated semen samples with progressive motility >70% and mo

APA, Harvard, Vancouver, ISO, and other styles

45

Solopchuk, Mariya S., Nelya S. Grigoryan, Naum A. Asnis, Tigran A. Vagramyan, Polina O. Shmelkova, and Olga I. Bardina. "STABILIZERS OF SOLUTIONS OF CHEMICAL COPPER PLATING." ChemChemTech 66, no. 3 (2023): 100–107. http://dx.doi.org/10.6060/ivkkt.20236603.6708.

Full text

Abstract:

The work is devoted to the study of the effect of various compounds on the stability of electroless copper plating solution containing Trilon B. as a chelating agent. The stability of electroless copper plating solutions was estimated by the accelerated method as the time before the start of intensive decomposition of the solution after the introduction of 0.15 ml of colloidal palladium activator solution heated to 75 °C in 250 ml. As stabilizers, both known, currently used, and newly selected compounds were investigated. Sulfur- and/or nitrogen-containing organic and inorganic compounds such

APA, Harvard, Vancouver, ISO, and other styles

46

Muñoz-Hernandez, Nadia Carolina, Claudia Karina Lagunes-Moreno, Manuel Barrientos-Villeda, et al. "Environmental Influences on Frozen Epididymal Sperm Cell Viability in White-Tailed Deer." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 13 (2024): 301–11. http://dx.doi.org/10.56557/upjoz/2024/v45i134157.

Full text

Abstract:

The objective of this study was to evaluate the association between Temperature and Humidity Index (THI), age, and post-collection time with post-thawing sperm cell parameters such as progressive motility, viability, morphology, and cytoplasmic droplets. Samples were collected from 4 male white-tailed deer after regulated hunting. THI was calculated on collection days. Two THI categories were determined, ≥70 and <70. Thawed semen was placed on a slide and observed under a phase-contrast microscope to evaluate the progressively motile sperm cells. Morphology, viability, and cytoplasmic dropl

APA, Harvard, Vancouver, ISO, and other styles

47

Kim, Eung Yeop, Young Hee Sung, and Jongho Lee. "Nigrosome 1 imaging: technical considerations and clinical applications." British Journal of Radiology 92, no. 1101 (2019): 20180842. http://dx.doi.org/10.1259/bjr.20180842.

Full text

Abstract:

A pathological study by Damier et al demonstrated that nigrosome 1, a dopaminergic neuron-rich region in the substantial nigra, is the most severely affected region in idiopathic Parkinson’s disease. Since then, researchers have identified the location of nigrosome 1 in the dorsal aspect of the substantia nigra using susceptibility-weighted imaging in MRI. Although this observation was reconfirmed by various imaging techniques and imaging planes, non-standardized imaging methods may rather limit the generalized use of this imaging finding. The aim of this review is to revisit the anatomical de

APA, Harvard, Vancouver, ISO, and other styles

48

Radhakrishna, D., D. Suneetha, and P. Shyamala. "Cloud Point Extraction and Spectrophotometric Studies for the Extraction of Nigrosin and Aniline Blue Dyes using Mixed Micelles of TBAB and Triton X-114." Asian Journal of Chemistry 33, no. 6 (2021): 1351–56. http://dx.doi.org/10.14233/ajchem.2021.23198.

Full text

Abstract:

The extractions of two anionic dyes viz. nigrosin and aniline blue by cloud point extraction method using mixed micelles of tetrabutylammonium bromide (TBAB) and Triton X-114 were conducted. To get the greatest extraction efficiency, pH of the solution, surfactant concentration, concentration of electrolyte, equilibrium temperature and time were optimized. A linear calibration curve in the range of 0.567-9.07 mg/L (nigrosin) and 0.982-19.645 mg/L (aniline blue) was obtained. Limit of detection were found to be 2.45 ng/L and 2.008 ng/L for nigrosin and aniline blue dyes, respectively. Thermodyn

APA, Harvard, Vancouver, ISO, and other styles

49

Kondracki, Stanisław, Anna Wysokińska, Magdalena Kania, and Krzysztof Górski. "Application of two staining methods for sperm morphometric evaluation in domestic pigs." Journal of Veterinary Research 61, no. 3 (2017): 345–49. http://dx.doi.org/10.1515/jvetres-2017-0045.

Full text

Abstract:

Abstract Introduction: The effect of two smear staining methods on the dimensions and shape of sperm cells in the semen of domestic pigs was evaluated. Material and Methods: The studies were carried out on 30 ejaculates collected from 15 boars, which included five Duroc boars, five Pietrain boars, and five hybrid Duroc × Pietrain boars. Each ejaculate was next sampled to make two microscopic slides, of which one was stained with eosin-nigrosin and the other with eosin-gentian dye. In total, 600 measurements of sperm cells were made. Each sperm was measured for the following morphometric parame

APA, Harvard, Vancouver, ISO, and other styles

50

Ardhani, Fikri, Julinda R. Manullang, and Bryta Mbincar Boangmanalu. "Abnormalitas Morfologi Spermatozoa Ayam Nunukan Asal Ejakulat." Jurnal Pertanian Terpadu 7, no. 1 (2019): 122–30. http://dx.doi.org/10.36084/jpt..v7i1.188.

Full text

Abstract:

Ayam nunukan merupakan plasma nutfah Indonesia yang berkembang di wilayah Kalimantan terutama bagian Utara dan Timur. Tingginya persentase abnormalitas morfologi spermatozoa akan menurunkan nilai fertilitas. Penelitian ini bertujuan untuk mengidentifikasi abnormalitas bentuk dan akan dikembangkan bahan identifikasi abnormalitas melalui pengecatan menggunakan kombinasi eosin nigrosin. Sepuluh ekor ayam nunukan jantan diatas satu tahun dengan bobot 2-2,5 kg dikoleksi semennya dengan metode massage atau pengurutan bagian punggung (dorsal) ayam jantan. Pembuatan preparat ulas dilakukan dengan meng

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!