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Relevant bibliographies by topics / Nitrate reductase assay

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Academic literature on the topic 'Nitrate reductase assay'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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Journal articles on the topic "Nitrate reductase assay"

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Schild, Johannes, and Jobst-Heinrich Klemme. "Enzymatic Nitrate Assay by a Kinetic Method Employing Escherichia coli Nitrate Reductase." Zeitschrift für Naturforschung C 40, no. 1-2 (1985): 134–37. http://dx.doi.org/10.1515/znc-1985-1-226.

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Abstract An enzymatic assay system for nitrate employing the membrane-bound nitrate reductase (EC 1.7.99.4) of E. coli is described. Contrary to previous enzymatic assay systems, the present method is a kinetic one, i.e. the substrate, nitrate, is assayed by measuring the reaction rate of the nitrate reductase-catalyzed reaction. Based on the observation that the nitrate reductase-catalyzed reaction obeys pseudo-first order kinetics, a test system is described allowing the assay of nitrate at a concentration as low as 1 ppm. The relatively high M ichaelis-M enten constant for nitrate (0.3 mᴍ)

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Giovannoni, G., J. M. Land, G. Keir, E. J. Thompson, and S. J. R. Heales. "Adaptation of the Nitrate Reductase and Griess Reaction Methods for the Measurement of Serum Nitrate plus Nitrite Levels." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 34, no. 2 (1997): 193–98. http://dx.doi.org/10.1177/000456329703400212.

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Nitrite and nitrate determinations in biological fluids are increasingly being used as markers of nitric oxide production. We have modified a nitrate reductase and Griess reaction method for the measurement of serum nitrate and nitrite in ultrafiltrated samples using a microtitre plate. The recoveries of nitrate and nitrite were 95% (range = 86–113%) and 100% (range = 92–109%), respectively. The intra and inter assay coefficients of variation for nitrate plus nitrite in the concentration range 40–50μM were 9·1% and 7·8%, and in the concentration range of 2·5–10μM 23·4% and 25·5%, respectively.

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Gomes, Dalila, Betânia Leite, and Fabiana Araujo Figueiredo da Mata. "PD64 Diagnostic Accuracy Of The Nitrate Reductase Assay Technique." International Journal of Technology Assessment in Health Care 34, S1 (2018): 153. http://dx.doi.org/10.1017/s0266462318003252.

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Introduction:The conventional drug sensitivity test is traditionally used in Brazil to diagnose drug-resistant tuberculosis. However, the test can take up to 60 days to return a diagnosis, which is considered too long for certain vulnerable populations. Therefore, this study analyzed the available scientific evidence on the accuracy and time to diagnosis of the nitrate reductase assay for diagnosing resistant tuberculosis, compared with the conventional drug sensitivity test.Methods:We searched MEDLINE, Embase, and The Cochrane Library for systematic reviews with meta-analyses. The articles we

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Jones, Alison M., and Roger Knowles. "Denitrifïcation in Flexibacter canadensis." Canadian Journal of Microbiology 36, no. 6 (1990): 430–34. http://dx.doi.org/10.1139/m90-075.

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Denitrifïcation was studied in pure cultures of Flexibacter canadensis (ATCC 29591), a Gram-negative gliding bacterium found in soil. Flexibacter canadensis was capable of using nitrate, nitrite, and nitrous oxide as terminal electron acceptors for growth. Sodium sulfide (200 μM) inhibited all of the nitrogen oxide reductases, but only temporarily. Acetylene (4 kPa) inhibited nitrous oxide reduction but did not affect the reduction of either nitrate or nitrite. However, sulfide (100 and 200 μM) alleviated the acetylene block and permitted reduction of nitrous oxide in the presence of 4 kPa ace

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Malm, Sven, Yvonne Tiffert, Julia Micklinghoff, et al. "The roles of the nitrate reductase NarGHJI, the nitrite reductase NirBD and the response regulator GlnR in nitrate assimilation of Mycobacterium tuberculosis." Microbiology 155, no. 4 (2009): 1332–39. http://dx.doi.org/10.1099/mic.0.023275-0.

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Mycobacterium tuberculosis can utilize various nutrients including nitrate as a source of nitrogen. Assimilation of nitrate requires the reduction of nitrate via nitrite to ammonium, which is then incorporated into metabolic pathways. This study was undertaken to define the molecular mechanism of nitrate assimilation in M. tuberculosis. Homologues to a narGHJI-encoded nitrate reductase and a nirBD-encoded nitrite reductase have been found on the chromosome of M. tuberculosis. Previous studies have implied a role for NarGHJI in nitrate respiration rather than nitrate assimilation. Here, we show

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Karlov, G. I., D. Y. Litvinov, P. N. Kharchenko, et al. "Adaptation of nitrate reductase activity assay for high throughput screening of crops." Siberian Herald of Agricultural Science 49, no. 6 (2020): 23–33. http://dx.doi.org/10.26898/0370-8799-2019-6-3.

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The possibility of freeze drying of plant material and its grinding in a shaking bead mill to determine the activity of nitrate reductase (NR) was studied. The effectiveness of applying this approach to high throughput mass screening of crops was shown. The assay was carried out on seedlings of common wheat (Triticum aestivum) of the following cultivars: Altigo, Vassa, Grom, Doka, Soberbash, Starshina, Fisht and spring wheat Novosibirskaya 67. The crops were grown during 4-5 weeks on substrate without nitrogen and on substrate supplemented with 50 millimol / l (mM) of potassium nitrate. Nitrat

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McNally, Niamh, Xiang Yang Liu, and Prabhakara V. Choudary. "A high-throughput assay format for determination of nitrate reductase and nitrite reductase enzyme activities." Applied Biochemistry and Biotechnology 62, no. 1 (1997): 29–36. http://dx.doi.org/10.1007/bf02787981.

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Hochman, Ayala, Aliza Nissany, David Wynne, Bina Kaplan, and Thomas Berman. "Nitrate reductase: an improved assay method for phytoplankton." Journal of Plankton Research 8, no. 2 (1986): 385–92. http://dx.doi.org/10.1093/plankt/8.2.385.

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Soussana, J. F., A. Gojon, L. Passama, R. Wakrim, and P. Robin. "Critical evaluation of thein situ nitrate reductase assay." Plant and Soil 120, no. 2 (1989): 243–51. http://dx.doi.org/10.1007/bf02377074.

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Singh, Sarman, Parveen Kumar, Shreya Sharma, Francis Mumbowa, Anandi Martin, and Nicolas Durier. "Rapid Identification and Drug Susceptibility Testing of Mycobacterium tuberculosis: Standard Operating Procedure for Non- Commercial Assays: Part 2: Nitrate Reductase Assay v1.3.12." Journal of Laboratory Physicians 4, no. 02 (2012): 112–19. http://dx.doi.org/10.4103/0974-2727.105593.

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ABSTRACTIn the previous part, we presented the standard operating procedure (SOP) of the microscopic observation drug susceptibility assay drug susceptibility testing (DST) for Mycobacterium tuberculosis. The present SOP is devoted to another non-commercial culture and DST method known as nitrate reductase assay (NRA). As the name implies, the NRA detects the ability of M. tuberculosis to reduce nitrate to nitrite. In the assay, the presence of nitrite is detected by the addition of p-nitrobenzoate into the growth yield. The reaction is detected by the naked eye. The incorporation of drugs in

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Book chapters on the topic "Nitrate reductase assay"

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Notton, B. A., P. N. Whitford, and R. J. Fido. "Quantitation of Nitrate Reductase Protein by Enzyme-Linked Immunosorbent Assay (ELISA) with Monoclonal Antibodies." In Inorganic Nitrogen Metabolism. Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-71890-8_15.

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Chow, Fungyi. "Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor." In Applied Photosynthesis. InTech, 2012. http://dx.doi.org/10.5772/26947.

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Conference papers on the topic "Nitrate reductase assay"

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Zhu, Xiang Y., Alvin Ayala, Hetal Modi, and John J. Kilbane. "Application of Quantitative, Real-Time PCR in Monitoring Microbiologically-Influenced Corrosion (MIC) in Gas Pipelines." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05493.

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Abstract Quantitative PCR (qPCR) methods (fluorogenic 5′-nuclease assay and SYBR Green I assay) were developed to detect and quantify bacteria and some critical sub-populations related to gas and oil pipeline corrosion. 16S rRNA genes were targeted for the detection and quantification of total bacteria using fluorogenic 5′-nuclease assays. The functional genes, dissimilatory sulfite reductase gene (dsrAB), nitrite reductase gene (nirS), and methyl-coenzyme M reductase gene (mcrA), were targeted for sulfate reducing bacteria (SRB), denitrifying bacteria, and methanognes, respectively. The metho

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Devasia, RA, A. Blackman, S. May, et al. "Fluoroquinolone Susceptibility Testing inM. tuberculosis: Comparison of Nitrate Reductase Assay and Agar Proportion Method." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a3198.

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