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1

Yuan, Yuan, Lin Zheng, and Chun Ying Zhou. "Acute Toxicological Effects of Suspended Solids on the Nitzschia closterium." Applied Mechanics and Materials 675-677 (October 2014): 120–25. http://dx.doi.org/10.4028/www.scientific.net/amm.675-677.120.

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In this paper, the acute toxicity test were used to study the effects of different concentrations of suspended solids on the growth of Nitzschia closterium. The research result showed that the effects of different concentration of suspended solids on the growth of Nitzschiaclosterium were very significantly (P <0.01);The relationship between concentration of suspended solids and the inhibition rate of Nitzschia closterium growth were linear, the half inhibition concentration of Nitzschia closterium 48h-EC50 was 4.27g/L,r=0.9329, 96 h-EC50 was 4.47g/L,r = 0.9388.
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2

Vardy, Suzanne, and Phillipa Uwins. "Fourier Transform Infrared Microspectroscopy as a Tool to Differentiate Nitzschia Closterium and Nitzschia longissima." Applied Spectroscopy 56, no. 12 (2002): 1545–48. http://dx.doi.org/10.1366/000370202321115788.

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Nitzschia closterium and Nitzschia longissima are two species of diatom that are extremely difficult to differentiate using light microscopy. This paper details an investigation into the use of FT-IR microscopy combined with discriminant analysis to differentiate between these species. Spectra were taken from unidentified samples and classified against a training set using either Mahalanobis distances or principal component analysis combined with canonical discriminant analysis. Unidentified samples were classified with a 100% accuracy using both mathematical techniques.
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3

Riouchi, Ouassila, Faid El Madani, Eric Abadie, Ali Skalli, and Mourad Baghour. "The spatio-temporal evolution of the genus Nitzschia Longissima at the level of the lagoon in Nador, Morocco." E3S Web of Conferences 234 (2021): 00081. http://dx.doi.org/10.1051/e3sconf/202123400081.

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This work aims to study the spatio-temporal evolution of the genus Nitzschia longissima, one of the most important genera of marine plankton diatoms, from 3 sampling stations in the Nador lagoon and during 2 seasons (spring and summer 2018), Using Nitzschia longissima, as a study system, one of the most diverse and abundant genera among marine planktonic diatoms. This species counts, in addition to the form Nitzschia longissima forma parva Grunow, three varieties namely Nitzschia longissima var. closterium (W. Smith) Van Heurck, Nitzschia longissima var. longissima (Breb.) Ralfs and Nitzschia longissima var. reversa Grunow. Nitzschia Longissima genus density was high during the warm season (Summer 2018) with a value of 8000 cells/liter, and low during the cold seasons (Spring 2018), which may be caused by water temperature and zooplankton community structure; and underwater light intensity was an important factor influencing the spatial distribution of Nitzschia density.
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4

Wang, Zhenyu, Bin Xia, Bijuan Chen, et al. "Trophic transfer of TiO2 nanoparticles from marine microalga (Nitzschia closterium) to scallop (Chlamys farreri) and related toxicity." Environmental Science: Nano 4, no. 2 (2017): 415–24. http://dx.doi.org/10.1039/c6en00365f.

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In the present study, the trophic transfer of TiO<sub>2</sub> NPs from marine microalga Nitzschia closterium to scallop Chlamys farreri was investigated. over the potential risk and environmental impact of manufactured nanoparticles (NPs) have increased in marine environments.
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5

Shi, Juan, KeHou Pan, XiaoQing Wang, et al. "Hierarchical recognition on the taxonomy of Nitzschia closterium f. minutissima." Chinese Science Bulletin 53, no. 2 (2008): 245–50. http://dx.doi.org/10.1007/s11434-008-0080-4.

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6

Florence, T. M., and J. L. Stauber. "Toxicity of copper complexes to the marine diatom Nitzschia closterium." Aquatic Toxicology 8, no. 1 (1986): 11–26. http://dx.doi.org/10.1016/0166-445x(86)90069-x.

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7

Li, Pei-Feng, Gui-Peng Yang, Jing Zhang, et al. "Impacts of elevated pCO2 on trace gas emissions in two microalgae: Phaeocystis globosa and Nitzschia closterium." Environmental Chemistry 14, no. 7 (2017): 425. http://dx.doi.org/10.1071/en17130.

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Environmental contextOcean acidification can affect marine microalgae, which can produce climate-active trace gases such as dimethylsulfide and various halocarbons. We conducted monoculture experiments simulating future ocean acidification, and showed that trace gas emissions are affected by elevated pCO2 to different degrees. The responses of trace gases to elevated pCO2 are compound- and species-specific. AbstractThe potential impacts of seawater acidification on the concentrations of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), dissolved acrylic acid (AAd) and various volatile halocarbons, including CH3Cl, CHBr3, CH2Br2, CHBr2Cl, CHBrCl2 and CH3I, were examined during a laboratory CO2 perturbation experiment for the microalgae Phaeocystis globosa and Nitzschia closterium. The microalgae were exposed to ambient CO2 conditions (390–540 µatm; 1 µatm = 0.1 Pa) and to projected concentrations for the end of the century (760–1000 µatm, high carbon (HC)). The growth rate of the two species remained unaffected by elevated CO2. Results showed a 48 and 37 % decline in the DMS concentration normalised to cell density in P. globosa and N. closterium cultures in the HC treatment compared with the ambient treatment. No significant difference was observed for DMSPp and DMSPd in the two microalgae cultures between the two CO2 levels. The mean AAd concentrations in the P. globosa culture showed a 28 % decline in the HC treatment. By contrast, the cell-normalised concentrations of AAd in the HC treatment were 45 % lower than in the ambient treatment in N. closterium cultures. No CO2-induced effects were observed for CH3Cl, CHBr3, CHBr2Cl, CHBrCl2 and CH3I, but cell-normalised concentrations of CH2Br2 in N. closterium cultures showed a 32 % decline in the HC treatment relative to the ambient level. These results show that the metabolism processes responsible for the production of climate-active gases in phytoplankton may be affected by high CO2 levels. There may be a potential delay in the responses of trace gas emissions to elevated pCO2.
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8

Stauber, J. L., and T. M. Florence. "Reversibility of copper-thiol binding in Nitzschia closterium and Chlorella pyrenoidosa." Aquatic Toxicology 8, no. 4 (1986): 223–29. http://dx.doi.org/10.1016/0166-445x(86)90074-3.

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9

Su, Xiaoling, Jilin Xu, Xiaojun Yan, et al. "Lipidomic changes during different growth stages of Nitzschia closterium f. minutissima." Metabolomics 9, no. 2 (2012): 300–310. http://dx.doi.org/10.1007/s11306-012-0445-1.

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10

Liu, Shu Xia, Yi Wan, and Dun Zhang. "Studies on Adhesion of Nitzschia closterium and Chlorella vulgaris to 316L Stainless Steel." Advanced Materials Research 485 (February 2012): 385–88. http://dx.doi.org/10.4028/www.scientific.net/amr.485.385.

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Microalgae can cause damages to the marine engineering material by adhering to the surface of the material. In this study the adhesion of Nitzschia closterium (bacillariophyceae) and Chlorella vulgaris (chlorophyceae) to 316L stainless steel were investigated. The influences of surface roughness and culture pH value on adhesion were performed. The results showed that the adhesion of N. closterium increased significantly with the increasing roughness (F = 17; p &lt; 0.001). And both species of algae were prone to adhesion to 316L stainless steel immerged in alkalescent medium. Impedimetric method was applied to analyze the process of microalgal adhesion on the surface of stainless steel. A kinetic model (Y = A + Be-t/C) based on the sensitive variation of double layer capacitance (Cdl) of the electrode abstracted from impedance spectroscopy using equivalent circuit was developed for the algal adhesion.
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11

Pablo, F., J. L. Stauber, and R. T. Buckney. "Toxicity of cyanide and cyanide complexes to the marine diatom Nitzschia closterium." Water Research 31, no. 10 (1997): 2435–42. http://dx.doi.org/10.1016/s0043-1354(97)00094-8.

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12

Yan, Xiaojun, Haiying Li, Jilin Xu, and Chengxu Zhou. "Analysis of phospholipids in microalga Nitzschia closterium by UPLC-Q-TOF-MS." Chinese Journal of Oceanology and Limnology 28, no. 1 (2010): 106–12. http://dx.doi.org/10.1007/s00343-010-9263-3.

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13

Engel, A., and M. Schartau. "Influence of transparent exopolymer particles (TEP) on sinking velocity of Nitzschia closterium aggregates." Marine Ecology Progress Series 182 (1999): 69–76. http://dx.doi.org/10.3354/meps182069.

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14

Yang, Rujun, Hongjie Tang, Yu Xin, and Xiulin Wang. "Size-dependent growth rate of Nitzschia closterium at different concentrations of petroleum hydrocarbon." Journal of Ocean University of China 8, no. 1 (2009): 45–50. http://dx.doi.org/10.1007/s11802-009-0045-5.

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15

Stewart, Michael, Craig Depree, and Karen J. Thompson. "Antifouling Sesterterpenes from the New Zealand Marine Sponge Semitaspongia Bactriana." Natural Product Communications 4, no. 3 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400306.

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Three antifouling (AF) sesterterpenes have been isolated from the New Zealand marine sponge Semitaspongia bactriana with toxicity against the diatom Nitzschia closterium and bryozoan Bugula neritina. The three metabolites have been characterised by spectroscopic techniques as 7 E, 12 E, 20 Z-variabilin (1), cavernosolide (2) and lintenolide A (3) (also called spongianolide C) and have low micromolar activity against our two test species. The γ-hydroxybutenolide containing sesterterpenes (2 and 3) show the most promise, with relative stability and suitable lipophilicity for incorporation of either these metabolites, or synthetic analogues, as biocides to produce paints or plastics with AF properties.
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16

Hernández -Almeida, O. U., and J. A. Herrera -Silva. "FIRST RECORD OF Reimerothrix floridensis (FRAGILARIACEAE: BACILLARIOPHYTA) FOR MÉXICO." CICIMAR Oceánides 29, no. 1 (2014): 29. http://dx.doi.org/10.37543/oceanides.v29i1.134.

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Primer registro de Reimerothrix floridensis (Fragilariaceae: Bacillariophyta) para México Reimerothrix floridensis forma parte de un grupo de diatomeas con forma arqueada que generalmente son confundidas con Cylindrotheca closterium, Psammosynedra closterioides y Nitzschia longissima var. reversa. En este trabajo se presenta el primer registro de la diatomea arqueada Reimerothrix floridensis , recolectada en Dzilam de Bravo (costa norte de la Península de Yucatán) en el extremo sur del Golfo de México. Este hallazgo se realizó en el marco de la identificación de las especies de diatomeas que conformaron una proliferación de fitoplancton suscitada en las costas de la Península de Yucatán durante 2011, y en donde C. closterium y N. longissima var. reversa fueron especies dominantes. Las características de Reimerothrix floridensis coinciden con la descripción original de la especie, excepto por las areolas asociadas al sternum. Dado que esta especie solo había sido registrada en la Bahía de Florida, su observación en México en la costa norte de la Península de Yucatán representa su registro más sureño, lo cual extiende el rango geográfico de esta especie. Por otra parte, debido a que la observación de Reimerothrix floridensis se realizó durante la presencia de una proliferación de C. closterium y N. longissima, especies con las que generalmente es confundida, se pone de manifiesto la importancia de realizar determinaciones taxonómicas cuidadosas acompañadas de descripciones detalladas que den certeza a los estudios ecológicos
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17

Li, Yan, Ya Hui Gao, Xue Song Li, Jin Ying Yang, and Guo He Que. "Influence of surface free energy on the adhesion of marine benthic diatom Nitzschia closterium MMDL533." Colloids and Surfaces B: Biointerfaces 75, no. 2 (2010): 550–56. http://dx.doi.org/10.1016/j.colsurfb.2009.09.026.

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18

Hogan, A. C., J. L. Stauber, F. Pablo, M. S. Adams, and R. P. Lim. "The Development of Marine Toxicity Identification Evaluation (TIE) Procedures Using the Unicellular Alga Nitzschia closterium." Archives of Environmental Contamination and Toxicology 48, no. 4 (2005): 433–43. http://dx.doi.org/10.1007/s00244-003-0137-y.

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19

Li, Na, Yu Liu, Zhengyu Liang, Yuxin Liu, and Yadi Lou. "Effect of petroleum hydrocarbons on the carbon and nitrogen stable isotope composition of Nitzschia closterium." IOP Conference Series: Earth and Environmental Science 446 (March 21, 2020): 032039. http://dx.doi.org/10.1088/1755-1315/446/3/032039.

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20

Xu, Wenjing, Liju Tan, Ting Zhao, Xiaolin Zhu, and Jiangtao Wang. "Toxicity assessments of acrylamide in aquatic environment using two algae Nitzschia closterium and Scenedesmus quadricauda." Environmental Science and Pollution Research 27, no. 16 (2020): 20545–53. http://dx.doi.org/10.1007/s11356-020-08581-y.

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21

Qi, Yarong, Xin Wang, and Jay Jiayang Cheng. "Preparation and characteristics of biosilica derived from marine diatom biomass of Nitzschia closterium and Thalassiosira." Chinese Journal of Oceanology and Limnology 35, no. 3 (2016): 668–80. http://dx.doi.org/10.1007/s00343-017-5329-9.

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22

Sinoir, Marie, Andrew R. Bowie, Mathieu Mongin, Edward C. V. Butler, and Christel S. Hassler. "Zinc requirement for two phytoplankton strains of the Tasman Sea." Marine and Freshwater Research 68, no. 2 (2017): 361. http://dx.doi.org/10.1071/mf15323.

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Zinc has been proposed as a limiting, or co-limiting, micronutrient for phytoplankton. In the Tasman Sea, extremely low zinc concentrations have been reported, raising the possibility there of limitation of phytoplankton growth by zinc. The pennate diatom Nitzschia closterium (CS-1) and the coccolithophorid Emiliania huxleyi (CS-812) were cultured in two low zinc concentrations (Zn2+ = 1.5 pmolL–1 and Zn2+ = 1.5 nmolL–1) mimicking conditions found in coastal and pelagic Tasman Sea. To monitor phytoplankton health and productivity, the maximum quantum yield (Fv/Fm), growth rate and cell size were analysed. These parameters showed that both strains were able to adapt and still grow. Short-term uptake experiments revealed an effect on Zn biological transport, with consequences for its bioavailability. When grown at low Zn2+ concentrations, E. huxleyi showed an induction of a two-transporter system, highly dependent on photosynthetic energy for Zn uptake. N. closterium was able to survive without inducing a higher-affinity Zn transporter. Its Zn uptake was also highly dependent on cellular energy and the ability to potentially access labile complexed forms of Zn. This strategy, thus, represented an advantage over E. huxleyi. Results are discussed in the context of the conditions found in the Tasman Sea.
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23

Gu, Xingyan, Keqiang Li, Kai Pang, Yunpeng Ma, and Xiulin Wang. "Effects of pH on the growth and NH4-N uptake of Skeletonema costatum and Nitzschia closterium." Marine Pollution Bulletin 124, no. 2 (2017): 946–52. http://dx.doi.org/10.1016/j.marpolbul.2017.01.048.

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24

Lou, Yadi, Yu Liu, Haixia Wang, et al. "Effects of CO2 restriction on growth of Nitzschia closterium: Evidence from stable isotopes and fatty acids." Ecotoxicology and Environmental Safety 177 (August 2019): 7–17. http://dx.doi.org/10.1016/j.ecoenv.2019.03.110.

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25

Li, Keqiang, Min Li, Yunfeng He, et al. "Effects of pH and nitrogen form on Nitzschia closterium growth by linking dynamic with enzyme activity." Chemosphere 249 (June 2020): 126154. http://dx.doi.org/10.1016/j.chemosphere.2020.126154.

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26

Stauber, J. L., and T. M. Florence. "The influence of iron on copper toxicity to the marine diatom, Nitzschia closterium (ehrenberg) W. Smith." Aquatic Toxicology 6, no. 4 (1985): 297–305. http://dx.doi.org/10.1016/0166-445x(85)90025-6.

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27

Zhou, Xiaojian, Jie Meng, Zhaowei Yu, Li Miao, and Cuili Jin. "The Alterations of Biofilm Formation and EPS Characteristics of a Diatom by a Sponge-Associated Bacterium Psychrobacter sp." Scientifica 2018 (June 24, 2018): 1–10. http://dx.doi.org/10.1155/2018/1892520.

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A sponge-associated bacterium, which was identified as Psychrobacter sp. in this study, was found with high activity against biofilm formation of benthic diatoms, including Amphora sp., Nitzschia closterium, Nitzschia frustulum, and Stauroneis sp. The activity against diatom biofilm formation by the tested strain was confirmed mostly in the culture supernatant and could be extracted using organic solvents. Treatment with its supernatant crude extract significantly reduced the cells of Stauroneis sp. forming biofilm and slightly increased the cells floating in the culture medium, which results in the ratio of biofilm cell/floating cell altering from 0.736 in control to 0.414 in treatment. Use of the supernatant crude extract led to increased production of extracellular polymeric substances (EPSs) by diatom Stauroneis sp. from 16.66 to 41.59 (g/g cell dry weight). The increase in EPS production was mainly contributed by soluble EPS (SL-EPS) and followed by the EPS that was tightly bound to biofilm cells (BF-TB-EPS). In addition, the supernatant crude extract caused significant changes in the monosaccharides composition of the EPS of Stauroneis sp. Specifically, glucuronic acid (Glc-A) and N-acetyl-D-glucosamine (Glc-NAc) in BF-TB-EPS were 55% fold decreased and 1219% fold increased, respectively. Based on our findings, we proposed that these changes in monosaccharides composition might lead to a decreased biofilm formation efficiency of diatom.
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28

Xia, Bin, Bijuan Chen, Xuemei Sun, Keming Qu, Feifei Ma, and Meirong Du. "Interaction of TiO 2 nanoparticles with the marine microalga Nitzschia closterium : Growth inhibition, oxidative stress and internalization." Science of The Total Environment 508 (March 2015): 525–33. http://dx.doi.org/10.1016/j.scitotenv.2014.11.066.

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29

Kuhnhenn, Verena, Jürgen Krägel, Ulrich Horstmann, and Reinhard Miller. "Surface shear rheological studies of marine phytoplankton cultures—Nitzschia closterium, Thalassiosira rotula, Thalassiosira punctigera and Phaeocystis sp." Colloids and Surfaces B: Biointerfaces 47, no. 1 (2006): 29–35. http://dx.doi.org/10.1016/j.colsurfb.2005.11.021.

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30

Liu, Wenhua, Zhongwen Huang, Ping Li, Jinfeng Xia, and Bin Chen. "Formation of triacylglycerol in Nitzschia closterium f. minutissima under nitrogen limitation and possible physiological and biochemical mechanisms." Journal of Experimental Marine Biology and Ecology 418-419 (May 2012): 24–29. http://dx.doi.org/10.1016/j.jembe.2012.03.005.

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31

FLORENCE, T., H. POWELL, J. STAUBER, and R. TOWN. "Toxicity of lipid-soluble copper(II) complexes to the marine diatom Nitzschia closterium: Amelioration by humic substances." Water Research 26, no. 9 (1992): 1187–93. http://dx.doi.org/10.1016/0043-1354(92)90179-8.

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32

Lei, Yanli, Chengchun Li, Tiegang Li, and Zhimin Jian. "The Effect of Temperature and Food Concentration On Ingestion Rates of Quinqueloculina Seminula On the Diatom Nitzschia Closterium." Journal of Foraminiferal Research 49, no. 1 (2019): 3–10. http://dx.doi.org/10.2113/gsjfr.49.1.3.

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Abstract The majority of sediment-dwelling foraminifera are thought to be deposit feeders. They use their reticulopodia to gather sediment with associated algae, organic detritus, and bacteria. Uptake of diatoms by foraminifera have been observed but rarely quantified. We measured the clearance (gathering) rate and ingestion rate of diatoms by the common benthic foraminifer Quinqueloculina seminula using Nitzschia closterium as prey under laboratory culture conditions. Grazing experiments were performed to evaluate the effects of temperature (at 12, 15, 18, 21, and 24°C) and food availability (10 to 800 cells mm−2) on uptake rates of diatoms. The clearance rates, estimated from the disappearance of food items, were variable (0.59–4.4 mm2 foram−1 h−1) and did not show a clear relationship with food availability. The maximum clearance rates increased from 1.80 ± 0.21 to 2.69 ± 0.32 mm2 foram−1 h−1 when temperature increased from 12 to 18°C and decreased to 2.28 ± 0.25 mm2 foram−1 h−1 at 24°C. Ingestion rates varied from 1.0 to 43 × 103 diatoms foram−1 h−1, following a hyperbolic response to food concentrations at all experimental temperatures. The maximum individual ingestion rates increased from 842 ± 180 to 1648 ± 480 (mean ± SE) cells foram−1 h−1 and then decreased to 316 ± 54 cells foram−1 h−1 as temperature increased from 12 to 24°C. Experimental results revealed that 12–18°C was the optimal temperature range for Q. seminula feeding for specimens adapted to local conditions. Our study indicates that Q. seminula plays an ecological role by feeding upon benthic diatoms in marine benthic ecosystems.
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Wang, Guizhong, Jie Xu, Qilong Jia, Chaoshu Zeng, Lisheng Wu, and Dingxun Wu. "Effects of microalgae as diets on the survival, development and fecundity of a pelagic cyclopoid copepod Apocyclops borneoensis." Journal of the Marine Biological Association of the United Kingdom 97, no. 6 (2016): 1251–57. http://dx.doi.org/10.1017/s0025315416000692.

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It has been proposed that the feeding habit of cyclopoids is different from that of calanoid copepods in that they feed mainly on microalgae during early development but become carnivorous later. However, a different view also exists, believing that microalgae are the prime food for some cyclopoid copepods. In the present study, microalgae from various taxonomic groups, including a dinoflagellate (Prorocentrum micans), three diatoms (Chaetoceros muelleri, Skeletonema costatum and Nitzschia closterium f. minutissima), and a prymnesiod (Isochrysis galbana), were offered at different concentrations to the cyclopoid copepod, Apocyclops borneoensis, with survival, development and reproduction of the copepod closely monitored. The results showed that A. borneoensis is capable of utilizing any of the microalgae species tested for development and reproduction, but significant differences in survival, development rates of both nauplii and copepodites, and fecundity were detected among species. The results also showed that within a same algal species, food concentration also significantly affected various biological parameters measured. Overall, C. muelleri and I. galbana were the better diets for A. borneoensis and their optimal food concentration ranged from 8.50 to 17.00 µg C ml−1. The optimal food concentration of P. micans was also found to be 8.50–17.00 µg C ml−1, however for the other two algae, S. costatum and N. closterium f. minutissima, it was lower at 1.70–8.50 µg C ml−1. The present study provides novel information on the feeding habit of A. borneoensis and the effects of both quality and quantity of microalgae diets on a range of biological parameters are described.
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Ryan, K. G., E. N. Hegseth, A. Martin, et al. "Comparison of the microalgal community within fast ice at two sites along the Ross Sea coast, Antarctica." Antarctic Science 18, no. 4 (2006): 583–94. http://dx.doi.org/10.1017/s0954102006000629.

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Diverse microbial communities survive within the sea ice matrix and are integral to the energy base of the Southern Ocean. Here we describe initial findings of a four season survey (between 1999–2004) of community structure and biomass of microalgae within the sea ice and in the underlying water column at Cape Evans and Cape Hallett, in the Ross Sea, Antarctica as part of the Latitudinal Gradient Project. At Cape Evans, bottom-ice chlorophyll a levels ranged from 4.4 to 173 mg Chl a m−2. Dominant species were Nitzschia stellata, N. lecointei, and Entomoneis kjellmanii, while the proportion of Berkeleya adeliensis increased steadily during spring. Despite being obtained later in the season, the Cape Hallett data show considerably lower standing stocks of chlorophyll ranging from 0.11 to 36.8 mg Chl a m−2. This difference was attributed to a strong current, which may have ablated much of the bottom ice biomass and provided biomass to the water below. This loss of algae from the bottom of the ice may explain why the ice community contributed only 2% of the standing stock in the total water column. Dominant species at Cape Hallett were Nitzschia stellata, Fragilariopsis curta and Cylindrotheca closterium. The low biomass at Cape Hallett and the prevalence of smaller-celled diatoms in the bottom ice community indicate that the ice here is more typical of pack ice than fast ice. Further data will allow us to quantify and model the extent to which ice-driven dynamics control the structure and function of the sea ice ecosystem and to assess its resilience to changing sea ice conditions.
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Li, Na, Yu Liu, Yuxin Liu, Yadi Lou, and Xinda Zhao. "Effect of oil spill on compound-specific stable carbon isotope composition of amino acid in Nitzschia closterium and Heterosigma akashiwo." Science of The Total Environment 653 (February 2019): 1095–104. http://dx.doi.org/10.1016/j.scitotenv.2018.11.021.

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36

Renaud, S. M., H. C. Zhou, D. L. Parry, Luong-Van Thinh, and K. C. Woo. "Effect of temperature on the growth, total lipid content and fatty acid composition of recently isolated tropical microalgae Isochrysis sp., Nitzschia closterium, Nitzschia paleacea, and commercial species Isochrysis sp. (clone T.ISO)." Journal of Applied Phycology 7, no. 6 (1995): 595–602. http://dx.doi.org/10.1007/bf00003948.

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37

Stauber, Jennifer L., Rodney J. Benning, Leigh T. Hales, Ruth Eriksen, and Barbara Nowak. "Copper bioavailability and amelioration of toxicity in Macquarie Harbour, Tasmania, Australia." Marine and Freshwater Research 51, no. 1 (2000): 1. http://dx.doi.org/10.1071/mf99010.

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The 100-year operation of the Mount Lyell Mining and Railway Company Limited’s copper mine in Queenstown, Australia, has resulted in the deposition of over 100 million cubic metres of mine tailings, smelter slag and topsoil into the King River and Macquarie Harbour. Apreliminary risk assessment, together with chemical measurements of dissolved copper, ASV-labile copper, copper complexing capacity and resin-adsorbed copper, suggested that copper in mid-salinity harbour waters was potentially bioavailable. However, toxicity tests based on inhibition of microalgal (Nitzschia closterium) growth showed that copper in these waters was not toxic, even though labile copper concentrations (6–24 µg L−1) exceeded the concentration of lowest observable effect for algae of 5 mg L−1. Measurements of intracellular and membrane-bound copper confirmed that cell division was not affected because copper was not taken up intracellularly. Amelioration of copper toxicity was due to binding of dissolved organic matter and/or other metals at the cell membrane, preventing copper binding and uptake. An understanding of the mechanism of copper toxicity and its amelioration is vital to assessing various clean-up options for the harbour.
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38

Harford, A. J., A. C. Hogan, J. J. Tsang, et al. "Effects of alumina refinery wastewater and signature metal constituents at the upper thermal tolerance of: 1. The tropical diatom Nitzschia closterium." Marine Pollution Bulletin 62, no. 3 (2011): 466–73. http://dx.doi.org/10.1016/j.marpolbul.2011.01.013.

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39

Stauber, J. L., and T. M. Florence. "The effect of culture medium on metal toxicity to the marine diatom Nitzschia closterium and the freshwater green alga Chlorella pyrenoidosa." Water Research 23, no. 7 (1989): 907–11. http://dx.doi.org/10.1016/0043-1354(89)90016-x.

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40

Stauber, J. L., and T. M. Florence. "Interactions of copper and manganese: A mechanism by which manganese alleviates copper toxicity to the marine diatom, Nitzschia closterium (Ehrenberg) W. Smith." Aquatic Toxicology 7, no. 4 (1985): 241–54. http://dx.doi.org/10.1016/0166-445x(85)90042-6.

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41

Koening, Maria Luise, Enide Eskinazi Leça, Sigrid Neumann-Leitão, and Silvio José de Macêdo. "Impacts of the construction of the Port of Suape on phytoplankton in the Ipojuca River estuary (Pernambuco-Brazil)." Brazilian Archives of Biology and Technology 46, no. 1 (2003): 73–82. http://dx.doi.org/10.1590/s1516-89132003000100012.

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In order to address the impact on phytoplankton, sampling was conducted monthly at 4 fixed stations, from April/86 to March/87 at diurnal low and high tide using a plankton net (65 mum mesh size) and a 1 L Van Dorn bottle. Among the 133 taxa identified, marine littoral euryhaline species were most common, outranking Gyrosigma balticum (Ehrenberg) Rabenhorst, Nitzschia sigma (Kützing) Wm. Smith, Licmophora abbreviata Agardh, Climacosphenia moniligera Ehrenberg, Surirella febigerii Lewis, Terpsinoe musica Ehrenberg and Cylindrotheca closterium (Ehrenberg) Reiman and Lewis. The port construction caused significant changes to the phytoplankton community with a strong influence of marine species (mainly dinoflagellate) because of the opening of the reef near the river mouth in 1983. The shallow depth and hydrodynamic brought many littoral species to the water columm. The community was composed by marine euryhaline and limnetic organisms, influenced by the salinity, rain and tide. Species diversity was high (&gt; 3 bits.cel-1) owing to the high environmental heterogeneity (marine, freshwater and benthic interactions). After the port implantation, a strong decrease occurred in phytoplankton density owing to high loads of suspended matter. Lowest values (121,00 cells.l-1) were registered during rainy season. During dry season, when light intensity was higher, phytoplankton presented highest density ( 1,789,000 cells.l-1).
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42

Wulff, Angela, Katharina Zacher, Dieter Hanelt, Adil Al-Handal, and Christian Wiencke. "UV radiation - a threat to Antarctic benthic marine diatoms?" Antarctic Science 20, no. 1 (2007): 13–20. http://dx.doi.org/10.1017/s0954102007000739.

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AbstractThis investigation was motivated by the lack of ultraviolet radiation (UVR, 280–400 nm) studies on Antarctic benthic marine microalgae. The objective was to estimate the impact of UV-B (280–315 nm) and UV-A (315–400 nm), on photosynthetic efficiency, species composition, cell density and specific growth rate in a semi-natural soft-bottom diatom community. In both experiments, cell density increased over time. The most frequently observed species were Navicula cancellata, Cylindrotheca closterium, Nitzschia spp., and Petroneis plagiostoma. For both experiments, a shift in species composition and a decreased photosystem II (PSII) maximum efficiency (Fv/Fm) over time was observed, irrespective of treatment. UVR significantly reduced Fv/Fm on days 3 and 10 (Expt 1), disappearing on the last sampling date. A similar trend was found in Expt 2. A significant UV effect on cell density was observed in Expt 1 (day 10) but not in Expt 2. No treatment effects on species composition or specific growth rate were found. Thus, the UV effects were transient (photosynthetic efficiency and cell density) and the growth of the benthic diatoms was generally unaffected. Overall, according to our results, UVR does not seem to be a threat to benthic marine Antarctic diatoms.
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43

Hu, Biyang, Qi Li, and Hong Yu. "RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation in Crassostrea gigas." International Journal of Molecular Sciences 22, no. 11 (2021): 6120. http://dx.doi.org/10.3390/ijms22116120.

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Porphyrins are a widespread group of pigments in nature which are believed to contribute to shell colors in mollusks. Previous studies have provided candidate genes for porphyrin shell coloration, however, the linkage analysis between functional genes and porphyrin pigmentation remains unclear in mollusks. RNA interference is a powerful molecular tool for analyzing the loss of functions of genes in vivo and alter gene expression. In this study, we used unicellular alga Platymonas subcordiformis and Nitzschia closterium f. minutissima as vectors to feed oysters with Escherichia coli strain HT115 engineered to express double-stranded RNAs targeting specific genes involved in porphyrin synthesis. A strain of Crassostrea gigas with orange shell was used to target key haem pathway genes expression using the aforementioned approach. We show here that feeding the oysters with E. coli, containing dsRNA targeting pigmentation genes, can cause changes in the color of the newly deposited shell. For example, the RNAi knockdown of CgALAS and CgPBGD resulted in the loss of uroporphyrin pigmentation from the shell due to the accumulation of the pigment in the oyster’s mantle. The study probed the crucial role of ALAS and PBGD genes potential functions of uroporphyrin production and shell color pigmentation in C. gigas.
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Sulistiono, A. Furkon, and R. Affandi. "(Study on Stomach Contents of Opudi Fish (Telmatherina celebensis) in Towuti Lake, South Sulawesi)." Jurnal Akuakultur Indonesia 5, no. 2 (2007): 149. http://dx.doi.org/10.19027/jai.5.149-156.

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&lt;p&gt;Study on food habits of opudi fish (&lt;em&gt;T. celebensis&lt;/em&gt;) was conducted in Towuti Lake, South Sulawesi, from October 2002 to April 2003. The objective of this study was to investigate food habits of the fish. Samples were collected by using experimental gill net, mesh size 0,75; 1; 1,25 and 1,5 inches. The fish (N=273) was consisted of 141 males and 132 females, varied in total body length (55.8-94.7 mm). Stomach content was analyzed using Index of preponderance. Stomach contents of the fish was consisted of 4 kinds of foods such as insect, part of organism, debris and zooplankton. Main food of the fish was insect (IP 51.22% for male, 43.26% for female, suplement food was part of insect (IP 26.99% for male, 30.27% for female) and debris (IP 14.06% for male, 15.83% for female), while additional food was zooplankton consisted of &lt;em&gt;Closterium&lt;/em&gt; (IP 2.93% for male, 4.62% for female), &lt;em&gt;Pinnularia&lt;/em&gt; (IP1.79% for male, IP 1.39% for female), &lt;em&gt;Navicula&lt;/em&gt; (IP 1.19%, for male, 1.41% for female) and &lt;em&gt;Nitzschia&lt;/em&gt; (IP 0.74% for male, 1.08% for female). Food kind between male and female fish was similar.&lt;/p&gt; &lt;p&gt;Keywords : Stomach contents, opudi fish (&lt;em&gt;T.celebensis&lt;/em&gt;), Towuti Lake.&lt;/p&gt; &lt;p&gt; &lt;/p&gt; &lt;p&gt;ABSTRAK&lt;/p&gt; &lt;p&gt;Penelitian makanan ikan opudi (&lt;em&gt;T.celebensis&lt;/em&gt;) dilakukan di Danau Towuti, Sulawesi Selatan pada bulan Oktober 2002-April 2003. Tujuan dari penelitian ini adalah untuk mengetahui isi lambung ikan opudi. Ikan contoh diambil dengan &lt;em&gt;experimental gill&lt;/em&gt; net dengan ukuran mata jaring 0,75; 1; 1,25 dan 1,5 inchi. Ikan yang tertangkap (N=273) terdiri atas Jantan (141) dan betina (132), yang memiliki variasi ukuran panjang tubuh (55,8-94,7 mm). Isi lambung dianalisis dengan menggunakan &lt;em&gt;Index of preponderance &lt;/em&gt;(IP). Isi lambung terdiri dari 4 jenis makanan yaitu insekta, potongan tubuh insekta, serasah dan zooplankton. Makanan utama ikan opudi adalah insekta (IP 51,22% untuk jantan, 43,26% untuk betina), makanan pelengkap adalah potongan insekta (IP 26,99% untuk jantan, 30,27% untuk betina) dan serasah (IP 14,06% untuk jantan, 15,83% untuk betina), sedangkan makanan tambahan berupa zooplankton yang terdiri atas&lt;em&gt; Closterium&lt;/em&gt; (2,93% untuk jantan, 4,62% untuk betina), &lt;em&gt;Pinnularia&lt;/em&gt; (1,79% untuk jantan, 1,39% untuk betina), &lt;em&gt;Navicula&lt;/em&gt; (1,19% untuk jantan, 1,41% untuk betina) and &lt;em&gt;Nitzschia&lt;/em&gt; (0,74% untuk jantan, 1,08% untuk betina). Berdasarkan indeks similaritas, jenis makanan untuk ikan jantan dan ikan betina adalah mirip.&lt;/p&gt; &lt;p&gt;Kata kunci : Isi lambung, ikan opudi (&lt;em&gt;T.celebensis&lt;/em&gt;), Danau Towuti.&lt;/p&gt;
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45

Huang, Jim Jun-hui, and Peter Chi-Keung Cheung. "+UVA treatment increases the degree of unsaturation in microalgal fatty acids and total carotenoid content in Nitzschia closterium (Bacillariophyceae) and Isochrysis zhangjiangensis (Chrysophyceae)." Food Chemistry 129, no. 3 (2011): 783–91. http://dx.doi.org/10.1016/j.foodchem.2011.05.021.

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46

Gan, Tingting, Nanjing Zhao, Gaofang Yin, et al. "Optimal chlorophyll fluorescence parameter selection for rapid and sensitive detection of lead toxicity to marine microalgae Nitzschia closterium based on chlorophyll fluorescence technology." Journal of Photochemistry and Photobiology B: Biology 197 (August 2019): 111551. http://dx.doi.org/10.1016/j.jphotobiol.2019.111551.

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47

周, 蜜., 娟. 石, 克厚 潘 та ін. "对小新月菱形藻(<italic>Nitzschia closterium </italic>f. minutissima)分类地位的重新认识". Chinese Science Bulletin 53, № 2 (2008): 197–202. http://dx.doi.org/10.1360/csb2008-53-2-197.

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48

Jones, GJ, SI Blackburn, and NS Parker. "A toxic bloom of Nodularia spumigena Mertens in Orielton Lagoon, Tasmania." Marine and Freshwater Research 45, no. 5 (1994): 787. http://dx.doi.org/10.1071/mf9940787.

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A bloom of Nodularia spumigena Mertens occurred in Orielton Lagoon, Tasmania, a shallow, eutrophic coastal embayment, between December 1992 and March 1993. The N. spumigena bloom was preceded by a eustigmatophyte bloom and was followed in March-April 1993 by a bloom of the diatoms Nitzschia closterium (Ehrenb.) Smith and Chaetoceros socialis Lauder. The Nodularia spumigena bloom may have been stimulated by low salinity (15-20 g kg-1) in the lagoon during December and January. Culture experiments with N. spumigena strains isolated from the lagoon showed best growth at salinities between 0 and 24 g kg-1 and less optimal growth at a salinity of 35 g kg-1. Akinete production in culture was positively correlated (P &lt; 0.001) with increasing salinity of growth media. The collapse of the N. spumigena population may have been triggered by decreasing water temperature in March, although this cannot be conclusively proven with the limited physico-chemical data available. High-performance liquid chromatographic (HPLC) analyses of bloom samples showed high concentrations (2000-3500 �g g-1 dry weight) of the cyclic pentapeptide hepatotoxin nodularin in samples collected during the peak of the N. spumigena bloom in January and February. Nodularin content of the bloom decreased as the population declined, owing to the decrease in abundance of N. spumigena and the release of nodularin by dying cells. A culture of N. spumigena isolated from Orielton Lagoon produced nodularin at concentrations comparable to those observed in field samples. A second HPLC peak, eluting very close to nodularin and with a similar ultraviolet spectrum, was observed in some field samples. This compound may be the ADDA-C8 stereoisomer of nodularin.
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49

Lavoie, I., F. V. Warwick, P. Reinhard, and J. Painchaud. "Effet du débit sur la dynamique temporelle des algues périphytiques dans une rivière influencée par les activités agricoles." Revue des sciences de l'eau 16, no. 1 (2005): 55–77. http://dx.doi.org/10.7202/705498ar.

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Le périphyton de la rivière Boyer Nord, une rivière affectée par les activités agricoles dans le sud du Québec (Canada), a été échantillonné toutes les deux semaines de la mi-mai à la fin septembre 1999 afin d'évaluer son évolution temporelle et d'identifier les variables potentielles qui le contrôlent. Les résultats montrent la grande variabilité temporelle de la biomasse périphytique (poids sec organique et chlorophylle a) et de la structure de la communauté de diatomées. La communauté d'algues benthiques dans la rivière Boyer était principalement composée de diatomées (Nitzschia, Cocconeis, Cymbella, Cyclotella), d'algues vertes (Scenedesmus, Pediastrum, Cosmarium, Closterium) et de cyanobactéries (Phormidium, Oscillatoria, Merismopedia). La pointe de débit observée durant la semaine précédant l'échantillonnage était fortement corrélée à plusieurs variables physico-chimiques (N-total, NH3-N, NO3-N, P-total, turbidité) et était le plus fortement corrélée aux changements temporels de la biomasse. La biomasse (chlorophylle a et poids sec organique) était négativement corrélée au phosphore total, ce qui reflète la relation avec le débit. Les changements temporels dans la composition spécifique des diatomées étaient régis par différentes variables physico-chimiques, selon les limites de tolérances et la valence écologique des espèces. Les algues périphytiques de cette communauté ont réagi aux variations de l'environnement à l'intérieur d'une période de 2 semaines puisque des changements majeurs dans la structure de l'assemblage de diatomées ont été observés lors de chaque échantillonnage. Ces observations montrent la forte variabilité de la biomasse et de la structure de la communauté périphytique dans les rivières enrichies par les éléments nutritifs et souligne l'influence majeure du débit dans ce type d'environnement.
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Ma, Qingwei, Ying Chen, Fanghui Wang, and Haowen Li. "Responses of Primary Productivity and Phytoplankton Community to the Atmospheric Nutrient Deposition in the East China Sea." Atmosphere 12, no. 2 (2021): 210. http://dx.doi.org/10.3390/atmos12020210.

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Atmospheric deposition of nutrients to the surface seawater may significantly affect marine phytoplankton growth. Two in situ bioassay experiments were performed in the East China Sea (ECS) by adding nutrients (N, P, and Si) and atmospheric aerosols into the surface seawater. Chlorophyll a (Chl a) concentrations were largely enhanced by simultaneous input of N and P with the maximal increase of 0.68–0.78 μg Chl a per μmol N addition. This Chl a increment was significantly lower (0.19–0.47 μg) in aerosol treatments as a result of initial N-replete condition (N/P ratio ~50) and extremely high N/P ratio in aerosols (&gt;300). Among the multiple influencing factors, atmospheric dry flux of NH4+ + NO3− (AN) was found to be an effective predictor for springtime Chl a in the ECS with a time lag of three days and were strongly correlated with Chl a concentrations on day 3 (r = 0.81, p &lt; 0.001), which might be partly explained by the asynchronous supplies of N (atmospheric deposition) and P (subsurface water). Although dinoflagellates dominated the phytoplankton community in both initial seawaters, additions of P and N + P + Si profoundly enhanced the cell densities and dominance of diatom species Thalassiosira sp. and Nitzschia closterium in the 2012 and 2014 bioassay experiments, respectively. Moreover, the percentage of dinoflagellates were promoted by adding higher NH4+/NO3− ratio (6/4 vs. 1/9) when silicate was at a low concentration (~2 μmol L−1). Atmospheric deposition is likely to be an important N source supporting the high primary production in the ECS and its supply of excess N relative to P may influence dominant phytoplankton groups.
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