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Journal articles on the topic 'Nodal culture'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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1

Humberto Estrella-Maldonado, Jesús R. Mora Solís, and Cynthia G. Rodríguez-Quibrera. "Disinfection procedure for stem cuttings and in vitro production of axillary buds for the Persian lime sanitation." International Journal of Science and Research Archive 7, no. 1 (2022): 470–76. http://dx.doi.org/10.30574/ijsra.2022.7.1.0223.

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The research work covered the in vitro establishment of Persian lime nodal segments using a twig disinfection protocol, and their culture on Murashige and Skoog (MS) culture media supplemented with antibiotics (Estrepto-Ler® and Rifampicin) and fungicide (Chlorothalonil). The axillary buds production was performed cultured the nodal segments without contamination signs on MS culture media enriched with two cytokinins (6-BAP and Kinetin) at different concentrations. Results showed that 52 % of the nodal segments did not show signs of in vitro contamination when the twig submerged in 20% chlorin
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2

Silveira, S. S., R. Cordeiro-Silva, J. Degenhardt-Goldbach, and M. Quoirin. "Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments." Brazilian Journal of Biology 76, no. 3 (2016): 656–63. http://dx.doi.org/10.1590/1519-6984.23714.

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Abstract Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenhouse. Mercury chloride and Plant Preservative Mixture™ were used in the surface sterilizing stage, better results being achieved with Plant Preservative Mixture™ incorporation in culture medium,
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3

Stamp, James A., Sheila M. Colby, and Carole P. Meredith. "Improved Shoot Organogenesis from Leaves of Grape." Journal of the American Society for Horticultural Science 115, no. 6 (1990): 1038–42. http://dx.doi.org/10.21273/jashs.115.6.1038.

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Adventitious shoots developed within 3 weeks from the petiolar stub and, less often, from wounded lamina tissues when leaves excised from nodal cultures of Vitis vinifera L. cvs. French Colombard and Thompson Seedless were cultured on solid Nitsch and Nitsch medium containing BAP at 2 mg·liter-1. The youngest leaf that could be excised, from 1 to 8 mm long, was the most responsive (90% of explants producing shoots compared to 16% for leaf 6). Removal of the lamina from the petiolar stub within the first 3 weeks of culture reduced shoot production. Increase in nodal culture age, without transfe
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Phongtongpasuk, Siriporn, and Phitchayakon Piemthongkham. "Shikimic Acid Production from Ginkgo biloba via Callus Culture." Advanced Materials Research 931-932 (May 2014): 1524–28. http://dx.doi.org/10.4028/www.scientific.net/amr.931-932.1524.

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Shikimic acid is a very important precursor for industrial synthesis of oseltamivir (Tamiflu®) which is used for the antiviral treatment. In this study, callus culture of Ginkgo biloba for shikimic acid production was reported. Callus induced from either leaves or nodal stems of sterilized ginkgo was grown on MS medium supplemented with a combination of plant growth regulators as followed: MS+KD, MS+BD, MS+KN and MS+BN for 90 days. Morphological changes, fresh weight and shikimic acid content of callus in each medium were monitored every 30 days. The result showed that callus cultures from eac
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5

Melyan, Gayane, Andranik Barsegyan, Narek Sahakyan, Kima Dangyan, and Yuri Martirosyan. "Development of in vitro culture establishment conditions and micropropagation of grapevine rootstock cultivar ‘Ruggeri-140’." BIO Web of Conferences 39 (2021): 03002. http://dx.doi.org/10.1051/bioconf/20213903002.

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Optimization of in vitro culture conditions of grapevine phylloxera-resistant rootstock cultivar ‘Ruggeri-140’(Vitisberlandieri x Vitisrupestris) was carried out. Among the different sterilization treatments, maximum aseptic cultures were obtained for both explants apical tips and nodal segments when treated with Ca(ClO)2 at concentration of 1.5 % for 10 minutes plus 70 % ethanol for 30 s (T7). The maximum shoot proliferation was observed both in apical and nodal meristems cultured on MS medium supplemented with 1.0 mg/l BAP. MS/2 medium containing 1.0 mg/l indole-3-butric acid (IBA) gave the
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6

Zhang, Zhen, and Zong-Ming Cheng. "The Effect of Jasmonic Acid on in Vitro Nodal Culture of Three Potato Cultivars." HortScience 31, no. 4 (1996): 631a—631. http://dx.doi.org/10.21273/hortsci.31.4.631a.

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Jasmonates are a group of native plant bioregulators that occur widely in the plant kingdom and exert various physiological activities when applied exogenously to plants. We investigated the effect of free jasmonic acid (JA) on stem and root growth and tuberization of potato in vitro nodal culture. Nodal cuttings of three potato cultivars, Norchip, Red Pontiac, and Russet Burbank, were cultured in 2.5 × 15 cm test tubes containing either nodal culture (MS with 2% sucrose) or tuber-inducing (MS with 8% sucrose and 11.5 μm kinetin) medium. The media were supplemented with JA at 0, 0.1, 0.5 1.0,
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7

Alves, André, and Wyatt Niehaus. "The Nodal Closet." International Journal of Art, Culture and Design Technologies 1, no. 2 (2011): 38–43. http://dx.doi.org/10.4018/ijacdt.2011070104.

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The synthesis of this text comes from a contemporary appraisal of the term “the closet” and its current place in culture since the advent of cybernetics. The authors’ assertion is that this metaphor, one that is rooted in the iconography of the late-capitalist home, is in a moment of transition and constant redefinition. In this paper, the authors explore and assess three basic modern manifestations of the homosexual rite of passage and how they have been rendered obsolete, adapted, or in some respects, catalyzed by new media and mobile technology. The charting of these changes is evaluated as
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8

Palla, Kaitlin J., Rochelle R. Beasley, and Paula M. Pijut. "In Vitro Culture and Rooting of Diospyros virginiana L." HortScience 48, no. 6 (2013): 747–49. http://dx.doi.org/10.21273/hortsci.48.6.747.

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The hard, strong, very close-grained wood of common persimmon (Diospyros virginiana L.; Ebenaceae) is desirable for specialty products such as golf club heads, percussion sticks, billiard cues, and for wood turnery. The edible fruit of cultivated varieties is sold as pulp for use in puddings, cookies, cakes, and custards. Persimmon is usually propagated by grafting. Own-rooted clonal persimmon could offer several advantages to specialty fruit growers such as elimination of grafting, graft incompatibility issues, and improved rootstocks for variety testing. Four mature, grafted (male and female
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9

Marin, M. L., and N. Duran-Vila. "Conservation of Citrus Germplasm in Vitro." Journal of the American Society for Horticultural Science 116, no. 4 (1991): 740–46. http://dx.doi.org/10.21273/jashs.116.4.740.

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A study was conducted to evaluate the potential of in vitro techniques for genetic conservation of citrus. A tissue culture system was developed using explants of juvenile `Pineapple' sweet orange. It consisted of: a) establishment of primary cultures from nodal stem segments followed by the recovery of plants in vitro; and b) successive cycles of secondary cultures consisting of the culture of nodal stem segments from in vitro-grown plants, rooting of shoots obtained from nodal stem segments, and recovery of whole plantlets. Two parameters, K and K', based on the multiplication factors of the
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10

Hashim, Saiyidah Nafisah, Siti Zulaiha Ghazali, Norrizah Jaafar Sidik, Tay Chia-Chay, and Azani Saleh. "Surface sterilization method for reducing contamination of Clinacanthus nutans nodal explants intended for in-vitro culture." E3S Web of Conferences 306 (2021): 01004. http://dx.doi.org/10.1051/e3sconf/202130601004.

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Surface sterilization is a vital step in preparation of healthy and viable explants in tissue culture. Most surface contaminants can be eliminated by surface sterilization with a suitable sterilizing agent. The study aimed to present an effective disinfection method for Clinacanthus nutans shoot regeneration using nodal segments. A total of four different sterilization approaches were conducted by treating nodal explants with various concentrations of sterilizing agent. Sterilizing agents used were Rhizophora apiculata Pyroligneous acid (PA), sodium hypochlorite (Clorox) thiophanate-methyl (fu
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11

Sharma, Vandana, SK Gupta, and Manjul Dhiman. "Regeneration of Plants from Nodal and Internodal Segment Cultures of Ephedra gerardiana using Thidiazuron." Plant Tissue Culture and Biotechnology 22, no. 2 (2013): 153–61. http://dx.doi.org/10.3329/ptcb.v22i2.14205.

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Ephedra gerardiana is an important medicinal plant used as Soma in Indian Ayurvedic system of medicine and as Traditional Chinese Medicine since several thousand years. Excessive use of this plant has led to decline in its natural population. Present report describes the use of TDZ in MS for induction of somatic embryos and shoot buds in internodal and nodal segment cultures of E. gerardiana. Somatic embryos were obtained from internodal segment culture onto MS + 0.5 to 1 ?M TDZ. In case of nodal segment culture, lower concentrations of TDZ induced only shoot buds in 63.33 - 88.88 per cent cul
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12

Yan, Yu-Ting, Jan-Jan Liu, Yi Luo, et al. "Dual Roles of Cripto as a Ligand and Coreceptor in the Nodal Signaling Pathway." Molecular and Cellular Biology 22, no. 13 (2002): 4439–49. http://dx.doi.org/10.1128/mcb.22.13.4439-4449.2002.

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ABSTRACT The EGF-CFC gene Cripto encodes an extracellular protein that has been implicated in the signaling pathway for the transforming growth factor beta (TGFβ) ligand Nodal. Although recent findings in frog and fish embryos have suggested that EGF-CFC proteins function as coreceptors for Nodal, studies in cell culture have implicated Cripto as a growth factor-like signaling molecule. Here we reconcile these apparently disparate models of Cripto function by using a mammalian cell culture assay to investigate the signaling activities of Nodal and EGF-CFC proteins. Using a luciferase reporter
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13

Rey, H. Y., and L. A. Mroginski. "Regeneration of Plants from Apical Meristem Tips and Nodal Segments of Arachis pintoi." Peanut Science 30, no. 2 (2003): 75–79. http://dx.doi.org/10.3146/pnut.30.2.0001.

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Abstract The in vitro regeneration potential of shoot apical tips (2 to 3 mm in length), meristems (0.3 to 0.5 mm in length), and nodal segments (4 to 7 mm long with an axillary bud) of diploid (2n = 2x = 20) and triploid (2n = 3x = 30) cytotypes of Arachis pintoi was evaluated. Explants were cultured on MS medium supplemented with different concentrations and combinations of naphthaleneacetic acid (NAA) and benzyladenine (BA). In one experiment the effect of gibberellic acid was tested. The cultures were done in liquid and solid media. Plant regeneration can be readily achieved from all expla
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14

Klčová, L., and M. Gubišová. "Evaluation of different approaches to buckwheat (Fagopyrum esculentum Moench.) micropropagation." Czech Journal of Genetics and Plant Breeding 44, No. 2 (2008): 66–72. http://dx.doi.org/10.17221/2677-cjgpb.

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Plant regeneration by different techniques was evaluated in three buckwheat cultivars: indirect regeneration from cotyledons and hypocotyls, direct regeneration by nodal segment cultivation and induction of multiple shoots from seedling apices. Regenerated shoots were obtained in all procedures. The effects of BA (6-benzylaminopurine), media composition and gelling agent were tested. The regeneration efficiency of shoot apex culture was 2.65–3.33 nodal segments/explant. Cotyledon and hypocotyl segments produced 1.25–2.44 shoots per explant plated. Nodal segment cultivation
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15

King, Sandra M., and A. L. Morehart. "Tissue Culture of Osage-orange." HortScience 23, no. 3 (1988): 613–15. http://dx.doi.org/10.21273/hortsci.23.3.613.

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Abstract The influence of explant (shoot-tip, node, or internode), growth regulators, liquid pretreatment, and Murashige and Skoog (MS) salt concentrations on callus, shoot, and root production was determined for Osage-orange [Maclura pomifera (Raf.) Schneid]. Shoots proliferated from both shoot-tip (three shoots/explant) and nodal (two shoots/explant) sections, but not from internodes. Optimum growth regulator concentrations for shoot proliferation were 0.5 µm IBA, 6 µm BA, and 3 µm GA. A 48-hr pretreatment of liquid-modified MS medium (MMS) did not enhance shoot proliferation. Internode sect
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16

BANSAL, Yogendra K., and Tarun CHIBBAR. "Micropropagation of Madhuca latifolia Macb. through nodal culture." Plant Biotechnology 17, no. 1 (2000): 17–20. http://dx.doi.org/10.5511/plantbiotechnology.17.17.

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17

Khan, Salim, Barna Goswami, Shahina Akter, et al. "In vitro mass propagation of Piper betle L." Bangladesh Journal of Botany 48, no. 3 (2019): 559–66. http://dx.doi.org/10.3329/bjb.v48i3.47917.

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An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best respons
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18

Moreira, C. B., S. S. Lima, M. A. Esquibel, and A. Sato. "Solasodine accumulation in regenerated plants of Solanum torvum Sw." Revista Brasileira de Plantas Medicinais 12, no. 1 (2010): 73–79. http://dx.doi.org/10.1590/s1516-05722010000100011.

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A nodal segment culture was developed in order to assess Solanum torvum Sw. regeneration and solasodine levels. The influence of auxins (indoleacetic acid, 1-Naphthaleneacetic acid) and benzyl adenine on S. torvum growth in micropropagation was investigated. A nodal segment culture was initiated with seeds germinated in MS basal medium added of GA3 and grown in different concentrations of IAA, IAA + BAP and NAA + BAP. Sixty-day-old plants from the in vitro culture were collected, frozen and lyophilized; then, the methyl orange method was used to quantify solasodine for the spectrophotometric a
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19

Chamandoosti, Firoozeh. "Citrus TISSUE CULTURE WITH TWO DIFFERENT APPROACHES." International Journal of Biosciences and Biotechnology 8, no. 1 (2020): 19. http://dx.doi.org/10.24843/ijbb.2020.v08.i01.p03.

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Citrus is an important genius for economy and human health but a susceptible genius against biotic and abiotic stresses, so it needs to improved programs. Different basal media (MS, ½MA, ?MS and DKW) and different kind and concentrations of plant growth regulators i.e. BA, KIN, 2ip, ZE and TDZ (0 – 2 mg/l) and NAA, IAA and IBA (0 – 2 mg/l) added with 30 g/l sucrose, 3 g/l active charcoal and 7.5 g/l bacteriological agar] were used for organogenesis include shooting and rooting, also callusing from nodal explant of Citrus latifolia. MS medium supplemented with 1 mg/l BA, and 0.01 mg/l NAA is th
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Galvão, António, Dariusz Skarzynski та Graça Ferreira-Dias. "Nodal Promotes Functional Luteolysis via Down-Regulation of Progesterone and Prostaglandins E2 and Promotion of PGF2α Synthetic Pathways in Mare Corpus Luteum". Endocrinology 157, № 2 (2015): 858–71. http://dx.doi.org/10.1210/en.2015-1362.

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Abstract In the present work, we investigated the role of Nodal, an embryonic morphogen from the TGFβ superfamily in corpus luteum (CL) secretory activity using cells isolated from equine CL as a model. Expression pattern of Nodal and its receptors activin receptor A type IIB (ACVR2B), activin receptor-like kinase (Alk)-7, and Alk4, as well as the Nodal physiological role, demonstrate the involvement of this pathway in functional luteolysis. Nodal and its receptors were immune localized in small and large luteal cells and endothelial cells, except ACVR2B, which was not detected in the endothel
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Stein, Vanessa Cristina, Renato Paiva, Daiane Peixoto Vargas, Fernanda Pereira Soares, Eduardo Alves, and Gabriela Ferreira Nogueira. "Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn." Revista Árvore 34, no. 5 (2010): 789–96. http://dx.doi.org/10.1590/s0100-67622010000500004.

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Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin w
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Mantovani, Nilton César, Magali F. Grando, Aloisio Xavier, and Wagner C. Otoni. "In vitro shoot induction and multiplication from nodal segments of adult Ginkgo biloba plants." Horticultura Brasileira 31, no. 2 (2013): 184–89. http://dx.doi.org/10.1590/s0102-05362013000200003.

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The in vitro performance of herbaceous and woody nodal segments from adult plants and the effect of hydrolyzed casein (HC 500 mg L-1), kinetin (KIN; 6-furfurylaminopurine 0.46 and 4.65 µM) and activated charcoal (AC 1.5 g L-1) were evaluated upon new shoots induction and development, and to establish a system of in vitro propagation from adult plants of Ginkgo biloba. Woody nodal segments did not produce axillary shoots and presented 100% of bacterial and fungal contamination in culture. However, nodal segments from herbaceous shoots were successfully disinfected and displayed high in vitro mo
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Souquet, Benoit, Sophie Tourpin, Sébastien Messiaen, Delphine Moison, René Habert, and Gabriel Livera. "Nodal Signaling Regulates the Entry into Meiosis in Fetal Germ Cells." Endocrinology 153, no. 5 (2012): 2466–73. http://dx.doi.org/10.1210/en.2011-2056.

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The mechanisms regulating the entry into meiosis in mammalian germ cells remain incompletely understood. We investigated the involvement of the TGF-β family members in fetal germ cell meiosis initiation. Nodal, a member of the TGF-β family, and its target genes are precociously expressed in embryonic gonads and show sexual dimorphism in favor of the developing testis. Nodal receptor genes, Acvr2a and Acvr2b, Alk4, and Tdgf1/Cripto, were identified in male germ cells. Nodal itself, Tdgf1, and Lefty1 and Lefty2 are targets of Nodal signaling and were all found specifically expressed in male germ
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Singh, BM, C. Wawrosch, SD Joshi, and B. Kopp. "Micropropagation of Bauhinia variegata L. from Tissue Culture." Nepal Journal of Science and Technology 13, no. 1 (2013): 39–41. http://dx.doi.org/10.3126/njst.v13i1.7397.

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Bauhinia variegate L. is a multipurpose tree and its micropropagation holds great promise in agroforestry. The sterilized seeds were first inoculated on Murashige and Skoog (MS) medium. Nodal cuttings from these seedlings grown in vitro were used as explants for micropropagation. Nodal cutting inoculated on the medium with various concentrations of BAP (benzyl-aminopurine) and NAA (£- naphthalene acetic acid) and separately generated varied results. Best propagation was recorded on MS medium supplemented with 1.0 ?M BAP with 0.05 ?M NAA. Propagated plants were successfully acclimatized and roo
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Lee, Zun Yip, Amir Hamzah Ahmad Ghazali, Bee Lynn Chew, and Sreeramanan Subramaniam. "An Effective Disinfection Protocol for The Development of Tissue Culture Mango Plant (Mangifera indica L.) cv. Harumanis." Malaysian Applied Biology 54, no. 1 (2025): 12–23. https://doi.org/10.55230/mabjournal.v54i1.2936.

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Harumanis mango is one of the highest demand mango cultivars in Malaysia due to its exceptional sweetness and fabulous fragrance. However, the production of this mango cannot meet the market demand due to limited grafting activity and the limited number of seeds (only one harvest season per year). These make vegetative and non-vegetative propagation of Harumanis time and labour intensive. Micropropagation using tissue culture techniques is a reliable and effective alternative for mass in vitro propagation of Harumanis at a consistent and faster rate, producing clones of the mother plants. Neve
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Rawat, Megha, Tina, Manu Pant, and Gaurav Pant. "In vitro propagation of commercial rose variety using nodal explant culture." Environment Conservation Journal 26, no. 1 (2025): 39–44. https://doi.org/10.36953/ecj.30243159.

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The clonal propagation strategy of plant tissue culture can produce a large quantity of high-quality planting material in a relatively short time. The study focuses on mass propagation of roses using tissue culture techniques. The method is suitable for rose varieties with high commercial value, such as cut flowers and potted plants. Standardization of surface sterilization techniques involved treatment of explants with fungicides, antibiotics, and mercuric chloride solutions. We found that antibiotic treatment was essential to remove the bacterial infection, which could be due to endophytic b
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Carvalho, Cristina Paiva da Silveira, Diva Correia, Abdellatif Kemaleddine Benbadis, José Magno Queiroz Luz, and Adroaldo Guimarães Rossetti. "In vitro culture of Spondias mombin L. nodal segments." Revista Brasileira de Fruticultura 24, no. 3 (2002): 776–77. http://dx.doi.org/10.1590/s0100-29452002000300054.

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Spondias mombin L. shoot cultures were initiated from nodal explants taken from plants propagated by seeds. Explants coming from 4-6 months old plants, previously disinfected, were cultivated on WPM medium supplemented with a wide range of concentrations of BAP (0.0, 0.22, 0.44, 2.22 and 4.44 muM) and NAA (0.0, 0.27 and 2.70 muM). After four weeks, the responses obtained were axillary shoot and root formation. The first response were preferentially induced with the medium containing only BAP, regardless of the BAP concentration. The addition of NAA on medium reduced significantly axillary shoo
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Gheytasi, Sajjad, and Mohsen Hanif. "A Theory for Cultural Resistance: The Cases of L. M. Silko’s Ceremony and L. Erdrich’s Tracks." Interdisciplinary Literary Studies 25, no. 3 (2023): 378–402. http://dx.doi.org/10.5325/intelitestud.25.3.0378.

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ABSTRACT One critical issue facing subaltern cultures is how they react to the process of cultural assimilation. This article aims to analyze the characters in Leslie Marmon Silko’s Ceremony and Louise Erdrich’s Tracks, to see how they interpret and react to the “social norms.” The novels offer counter discourses that try to resist, negate, and put in suspense the nodal points of the dominant culture. When exposed to the hegemonic discourse, the subalterns also construct and re-territorialize the significance of the nodal points. The two novelists use elements from their own cultures in the fo
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Mangal, Anupam K., Arun M. Gurav, Ritu Sinha, Archana G. Mhase, and Gajendra Rao. "Conservation of Manjishtha—Rubia cordifolia L. through Nodal Culture." Journal of Drug Research in Ayurvedic Sciences 2, no. 4 (2017): 267–73. http://dx.doi.org/10.5005/jp-journals-10059-0022.

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Hirimburegama, Kshanika, and Niranjini Gamage. "Propagation ofBambusa vulgaris(yellow bamboo) through nodal bud culture." Journal of Horticultural Science 70, no. 3 (1995): 469–75. http://dx.doi.org/10.1080/14620316.1995.11515317.

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Jazib, Ahshan, Mohd Talim Hossain, and Raihan I. Raju. "Clonal propagation of Dracaena fragrans cv. Victoria through tissue culture technology." Jahangirnagar University Journal of Biological Sciences 8, no. 2 (2020): 1–11. http://dx.doi.org/10.3329/jujbs.v8i2.49833.

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Micropropagation of Dracaena fragrans cv.Victoria was conducted using the young, tender and disease-free leaves and nodal segments as explants collected from the local market of Savar, Dhaka. Surface sterilization of the explants pretreated with a liquid detergent and then 0.2% HgCl2 for 4-5 minutes produces maximum contamination free explants without any toxicity. After surface sterilization, different explants were inoculated on gelrite gelled MS medium supplemented with different concentrations of 2,4-D for callus induction and with different concentrations and combinations of BAP and NAA f
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Hayes, Kevin, Yun-Kyo Kim, and Martin F. Pera. "A Case for Revisiting Nodal Signaling in Human Pluripotent Stem Cells." Stem Cells 39, no. 9 (2021): 1137–44. http://dx.doi.org/10.1002/stem.3383.

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Abstract Nodal is a transforming growth factor-β (TGF-β) superfamily member that plays a number of critical roles in mammalian embryonic development. Nodal is essential for the support of the peri-implantation epiblast in the mouse embryo and subsequently acts to specify mesendodermal fate at the time of gastrulation and, later, left-right asymmetry. Maintenance of human pluripotent stem cells (hPSCs) in vitro is dependent on Nodal signaling. Because it has proven difficult to prepare a biologically active form of recombinant Nodal protein, Activin or TGFB1 are widely used as surrogates for NO
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Tan, Boon Chin, Chiew Foan Chin, and Peter Alderson. "An Improved Plant Regeneration of Vanilla planifolia Andrews." Plant Tissue Culture and Biotechnology 21, no. 1 (2012): 27–33. http://dx.doi.org/10.3329/ptcb.v21i1.9560.

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Multiple shoots of Vanilla planifolia were induced from nodal explants under influence of different concentrations of plant growth regulators and coconut water (CW). A comparison of shoot regeneration between semi solid and liquid culture media was also investigated. Ninety seven per cent of the explants produced a mean number of 9.6 shoots with a mean shoot length of 4.70 cm when cultured on liquid MS supplemented with 1.0 mg/l BAP in combination of 15% CW. Shoots generated were rooted with frequency of 93% on MS supplemented with 1.0 mg/l NAA with a mean number of 2.9 roots per shoot and mea
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Demidenko, Danaya V., Nataliya V. Varlamova, Taisiya M. Soboleva, Aleksandra V. Shitikova, and Marat R. Khaliluev. "An Efficient and Rapid Protocol for Somatic Shoot Organogenesis from Juvenile Hypocotyl-Derived Callus of Castor Bean cv. Zanzibar Green." BioTech 13, no. 3 (2024): 25. http://dx.doi.org/10.3390/biotech13030025.

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Aseptic seedlings of different ages derived from surface-sterilized mature seeds were applied as an explant source. Various explants such as 7- and 21-day-old hypocotyl fragments, 42-day-old nodal stem segments, and transverse nodal segments of stem, as well as leaf petioles, were cultured on the agar-solidified Murashige and Skoog (MS) basal medium supplemented with 0.1 mg/L IAA, 5 mg/L AgNO3 and different types and concentrations of cytokinin (1 mg/L zeatin, 0.25 mg/L thidiazuron (TDZ), and 5 mg/L 6-benzylaminopurine (6-BAP)). Consequently, it was found that 7- and 21-day-old hypocotyl fragm
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35

Markovic, Marija, Marija Popovic, and Dragica Vilotic. "Micropropagation of Dianthus deltoides L. through shoot tip and nodal cuttings culture." Archives of Biological Sciences 65, no. 1 (2013): 17–22. http://dx.doi.org/10.2298/abs1301017m.

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Micropropagation (shoot tip and nodal cuttings culture) was used for the rapid propagation of the non-invasive, decorative, native plants of maiden pink (Dianthus deltoides L.) in order to preserve their genetic diversity. In vitro culture was successfully established on Murashige and Skoog medium (MS) using seeds as the initial material. In the shoot multiplication phase, the explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA). The highest multiplication rate was achieved on a medium containing 0.1 mgL-1
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36

Biswas, Animesh, M. A. Bari, Mohashweta Roy, and S. K. Bhadra. "In vitro Propagation of Stemona tuberosa Lour. - A Rare Medicinal Plant through High Frequency Shoot Multiplication using Nodal Explants." Plant Tissue Culture and Biotechnology 21, no. 2 (2011): 151–59. http://dx.doi.org/10.3329/ptcb.v21i2.10238.

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Nodal segments of Stemona tuberosa Lour. were found to proliferated without any differentiation on to MS supplemented with 3.0 mg/l BAP and 0.5 mg/l NAA under continuous dark condition. After 20 days of inoculation under dark condition the cultures were transferred to a daily cycle of 16/8 hrs light/dark photoperiod and there the proliferated nodal segments underwent direct organogenesis producing huge number of shoot buds (25.87/culture). The shoot buds underwent rapid elongation on a range of BAP (0.1 - 1.5 mg/l) and IBA (0.1 - 1.0 mg/l) supplemented MS. Rooting of elongated shoot buds was s
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37

Downey, Cassandra D., Gregory Golenia, Ekaterina A. Boudko, and Andrew Maxwell P. Jones. "Cryopreservation of 13 Commercial Cannabis sativa Genotypes Using In Vitro Nodal Explants." Plants 10, no. 9 (2021): 1794. http://dx.doi.org/10.3390/plants10091794.

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Cannabis has developed into a multi-billion-dollar industry that relies on clonal propagation of elite genetics with desirable agronomic and chemical phenotypes. While the goal of clonal propagation is to produce genetically uniform plants, somatic mutations can accumulate during growth and compromise long-term genetic fidelity. Cryopreservation is a process in which tissues are stored at cryogenic temperatures, halting cell division and metabolic processes to facilitate high fidelity germplasm preservation. In this study, a series of experiments were conducted to optimize various stages of cr
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38

Iriondo, José M., Carmen Moreno, and César Pérez. "Micropropagation of Six Rockrose (Cistus) Species." HortScience 30, no. 5 (1995): 1080–81. http://dx.doi.org/10.21273/hortsci.30.5.1080.

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Micropropagation methods for six rockrose species (Cistus albidus L., C. clusii Dunal, C. ladanifer L., C. laurifolius L., C. psilosepalus L., and C. salvifolius L.) were established. Cultures, initiated from nodal segments of seedlings, were grown on MS medium, alone, or supplemented with 0.88 μm BAP or 0.93 μm Kin. Multiple shoot formation was obtained after the first subculture (30 days) from which new nodal segments were taken and grown on the same culture medium to maintain proliferation. Shoots obtained at the third subculture were rooted alone or supplemented with different concentratio
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39

PUNYARANI, Kshetrimayum, and Jitendra G. SHARMA. "Micropropagation of Costus speciosus (Koen.) Sm. Using Nodal Segment Culture." Notulae Scientia Biologicae 2, no. 1 (2010): 58–62. http://dx.doi.org/10.15835/nsb213552.

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40

Bello, Oluwakemi A., Edward B. Esan, and Olawole O. Obembe. "Establishing surface sterilization protocol for nodal culture of Solanecio biafrae." IOP Conference Series: Earth and Environmental Science 210 (December 6, 2018): 012007. http://dx.doi.org/10.1088/1755-1315/210/1/012007.

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41

Kausar, M., S. Parvin, ME Haque, M. Khalekuzzaman, B. Sikdar, and MA Islam. "Efficient Direct Organogenesis from Shoot Tips and Nodal Segments of Ash Gourd (Benincasa Hispida L.)." Journal of Life and Earth Science 8 (August 22, 2014): 17–20. http://dx.doi.org/10.3329/jles.v8i0.20135.

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The effect of external application of phytohormone on explants viz., shoot tips and nodal segments of ash gourd (Benincasa hispida L.) was tested on the frequency of shoot and root induction. Shoot tips and nodal segments were cultured on MS medium supplemented with different concentrations and combinations of cytokinins (BAP, Kinetin), auxin (IBA, NAA) and gibberellic acid (GA3) for multiple shoot formation and root induction. The highest number (up to 90%) of multiple shoot formation was obtained from the shoot tips in MS medium fortified with 1.5 mg/l BAP + 0.2 mg/l GA3, where average numbe
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42

Diez, J., and L. Gil. "Micropropagation of Ulmus minor and U. minor x U. pumila from 4-year-old ramets." Forest Systems 13, no. 1 (2004): 249–54. http://dx.doi.org/10.5424/829.

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As part of the Spanish breeding programme against Dutch elm disease, investigations were undertaken on reproduction of Ulmus minor by means of tissue culture. Microshoots were obtained from nodal segments, collected from terminal twigs of 4-year-old ramets of selected clones of this species and of U. minor x U. pumila, and cultured on a modified Murashige & Skoog medium containing 6-Benzylaminopurine (BA). When subcultured, the microshoots or nodal segments excised from these, formed new shoots, their maximum numbers per explant (1.94 to 2) being obtained with the hybrid clone M-TC2 on med
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43

Giles, Kenneth L., Kenneth Friesen, and Debra Novakovski. "THE USE OF IN VITRO MIST CULTIVATION FOR THE PROPAGATION AND HARDENING-OFF OF VIRUS-FREE POTATO PLANTLETS." HortScience 27, no. 6 (1992): 698b—698. http://dx.doi.org/10.21273/hortsci.27.6.698b.

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The use of an in vitro mist culture systems for the growth of virus free shoots of several potato cultivars has been demonstrated using the “Mystifier®”, several different media and growth regulator concentrations have been used to monitor the efficiency of shoot multiplication and growth. Nodal cuttings of sterile in vitro virus free cultures were used as inocula and the mist was applied at various rates in order to assess optimum sucrose concentrations and mineral concentrations. Murashige and Skoog medium was used and diluted to half and quarter strength. Hardening off the resulting shoots
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44

Cui, Jin, Juanxu Liu, Min Deng, Jianjun Chen, and Richard J. Henny. "Plant Regeneration through Protocorm-like Bodies Induced from Nodal Explants of Syngonium podophyllum ‘White Butterfly’." HortScience 43, no. 7 (2008): 2129–33. http://dx.doi.org/10.21273/hortsci.43.7.2129.

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Syngonium podophyllum ‘White Butterfly’, one of the most popular ornamental foliage plants, is propagated almost exclusively through in vitro shoot culture. Ex vitro rooting, however, has been associated with severe Myrothecium leaf spot (Myrothecium roridum Tode ex Fr.). The objective of this study was to establish a method for regenerating well-rooted plantlets before ex vitro transplanting. Leaf and petiole explants were cultured on a Murashige and Skoog (MS) basal medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU), N-phenyl-N′-1,2,3-thiadiazol-5-ylurea (TDZ), 6-benzyladen
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45

Jahan, I., N. Alam, and PK Roy. "Micropropagation of yardlong bean (Vigna unguiculata (L.) Walsp. ssp. Sesquipedalis L. Verdc.) through in vitro culture." Bangladesh Journal of Botany 44, no. 2 (2018): 345–50. http://dx.doi.org/10.3329/bjb.v44i2.38529.

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An in vitro propagation protocol has been established for yardlong bean (Vigna unguiculata ssp. sesquipedalis L. Walp. Verdc.) using shoot tip, nodal and leaf segment explants of the superior plant type obtained from crossing of two selected parental lines. Explants were cultured on MS medium supplemented with different concentrations and combinations of BAP, Kn and Zeatin and NAA, IAA, IBA. The highest percentage (86) of shoot regeneration with maximum shoots per culture (12 ± 0.43) was obtained from nodal explants on MS medium supplemented with 0.5 mg/l BAP and 0.2 mg/l NAA. Addition of 10%
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46

Rumeesa, Habib, Seema Singh Dr., and Bashir Iram. "Effect of cytokinins on in vitro propagation of Petunia hybrida Hort. Vilm." International Journal of Trend in Scientific Research and Development 2, no. 4 (2018): 1149–52. https://doi.org/10.31142/ijtsrd14163.

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An efficient in vitro propagation protocol has been developed in Petunia hybrida using nodes as explants. Both direct as well as indirect shoot regeneration was achieved. Direct shoot regeneration was obtained on MS basal medium after 7 days of inoculation with 50 culture response. Medium containing BAP 0.5 mg l resulted in direct shoot regeneration after 13 days of inoculation with 50 culture response. Best results were obtained on MS medium supplemented with BAP 1 mg l which resulted in indirect shoot regeneration in 70 cultures after 5 average number of days. Indirect shooting with 20 cultu
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47

Hassan, AKMS, CK Roy, R. Sultana, and R. Khatun. "Callus induction and plant regeneration of paederia foetida L., a widely used medicinal vine in Bangladesh." Bangladesh Journal of Scientific and Industrial Research 47, no. 4 (2013): 373–78. http://dx.doi.org/10.3329/bjsir.v47i4.14066.

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An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obtained when the callus was sub cultured on MS medium with 0.5 mg/L BAP. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/L IBA. The survival rate of plantlets was found to be 85%. Regen
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48

Hasan, MF, MS Rahman, MS Hossain, and M. Rahman. "Studies on In vitro Callus Induction and Regeneration from Nodal Explants of Cassia alata L." Progressive Agriculture 19, no. 1 (2013): 39–44. http://dx.doi.org/10.3329/pa.v19i1.17105.

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A successful protocol for adventitious shoot regeneration was developed from the nodal explant derived callus tissue of Cassia alata. Greenish friable callus was induced from the cut surface of the nodal explants on MS medium supplemented with 1.5 mgl-1 2,4-D within twenty days of inoculation. The calli differentiated into adventitious shoots when they were cultured on MS medium supplemented with 1.5 mgl-1 2,4-D and 0.5 mgl-1 Kn. In this treatment, the highest number of shoot induction per callus was 6.0 ±1.0. The callus derived shoots rooted on MS medium containing 1.0 mgl-1 IBA within ten da
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Moriguchi, Takaya, and Shohei Yamaki. "Prolonged Storage of Grape Nodal Culture Using a Low Concentration of Ammonium Nitrate." HortScience 24, no. 2 (1989): 372–73. http://dx.doi.org/10.21273/hortsci.24.2.372.

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Abstract A convenient method of conserving grape (Vitis labrusca L. cv. Delaware; F. thunbergii; V. vinifera L. cv. Rizamat) was developed by culturing nodal segments in Murashige and Skoog’s medium, but at a lower than normal concentration of ammonium nitrate under standard tissue culture conditions consisting of a 16-hr photo-period at 28C. The cultures had a higher rate of survival after 262 to 290 days of storage under low ammonium nitrate condition than under low temperature (5 or 10C).
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50

KANCHANAPOOM, Kantamaht, Suttinee JINGJIT, and Kamnoon KANCHANAPOOM. "In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39, no. 1 (2011): 84. http://dx.doi.org/10.15835/nbha3914994.

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A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium s
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