Academic literature on the topic 'Non-radioactive molecular hybridization'

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Journal articles on the topic "Non-radioactive molecular hybridization"

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Cremers, A. F. M., N. Jansen in de Wal, J. Wiegant, et al. "Non-radioactive in situ hybridization." Histochemistry 86, no. 6 (1987): 609–15. http://dx.doi.org/10.1007/bf00489555.

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Durrant, Ian. "Fluorescent Molecules as Direct and Indirect Labels for in situ Hybridization." Proceedings, annual meeting, Electron Microscopy Society of America 54 (August 11, 1996): 24–25. http://dx.doi.org/10.1017/s0424820100162582.

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In situ hybridization has traditionally been performed with radioactive probes. Over recent years effort has been spent in elucidating non-radioactive alternatives for a variety of molecular biology systems. Non-radioactive in situ hybridization is possible, but to date the systems available have, in general, delivered lower sensitivity and higher backgrounds than traditional methods. We have studied a variety of alternative labelling and detection processes; different systems may be required for different applications.Fluorescein has been developed as a new label for all aspects of non-radioa
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Wiegant, J. C. A. G., A. K. Raap, and M. van der Ploeg. "7 Methods for non-radioactive in situ hybridization." Cancer Genetics and Cytogenetics 38, no. 2 (1989): 155. http://dx.doi.org/10.1016/0165-4608(89)90535-9.

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KUMMERT, J., D. COLINET, and P. LEPOIVRE. "Detection of plant viruses by molecular hybridization using non-radioactive probes." EPPO Bulletin 25, no. 1-2 (1995): 301–13. http://dx.doi.org/10.1111/j.1365-2338.1995.tb01471.x.

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Khan, S. A., M. S. Nawaz, A. A. Khan, and C. E. Cerniglia. "Direct in-gel hybridization of digoxigenin-labelled non-radioactive probes." Molecular and Cellular Probes 13, no. 3 (1999): 233–37. http://dx.doi.org/10.1006/mcpr.1999.0241.

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Quibriac, M., J. Petitjean, V. Thiers, P. Tiollais, C. Brechot, and F. Freymuth. "Comparison of a non-radioactive hybridization assay for detection of hepatitis B virus DNA with the radioactive method." Molecular and Cellular Probes 3, no. 3 (1989): 209–12. http://dx.doi.org/10.1016/0890-8508(89)90001-7.

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Asinari, F., E. E. Cafrune, F. A. Guzman, L. R. Conci, and V. C. Conci. "Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry." AgriScientia 33, no. 1 (2016): 39–45. http://dx.doi.org/10.31047/1668.298x.v33.n1.16570.

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The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesi
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Bochenek, Birgit, and Ann M. Hirsch. "In-situ hybridization of nodulin mRNAs in root nodules using non-radioactive probes." Plant Molecular Biology Reporter 9, no. 3 (1991): 219. http://dx.doi.org/10.1007/bf02672070.

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Bochenek, Birgit, and Ann M. Hirsch. "In-Situ hybridization of nodulin mRNAs in root nodules using non-radioactive probes." Plant Molecular Biology Reporter 8, no. 4 (1990): 237–48. http://dx.doi.org/10.1007/bf02668761.

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Larsson, L. I., and D. M. Hougaard. "Optimization of non-radioactive in situ hybridization: image analysis of varying pretreatment, hybridization and probe labelling conditions." Histochemistry 93, no. 4 (1990): 347–54. http://dx.doi.org/10.1007/bf00315849.

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Dissertations / Theses on the topic "Non-radioactive molecular hybridization"

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Habeebu, Sahibu Sultan Murtaza. "The development of non-radioactive detection systems for molecular in situ hybridization." Thesis, University of Cambridge, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359750.

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Book chapters on the topic "Non-radioactive molecular hybridization"

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Bustos-Sanmamed, Pilar, Carole Laffont, Florian Frugier, Christine Lelandais-Brière, and Martin Crespi. "Analyzing Small and Long RNAs in Plant Development Using Non-radioactive In Situ Hybridization." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-221-6_20.

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Mullink, H., W. Vos, N. M. Jiwa, A. Horstman, E. Rieger, and C. J. L. M. Meijer. "Combination of non-radioactivein situ hybridization andimmunocytochemistry." In Non-isotopic Methods in Molecular Biology. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780199634552.003.0005.

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Abstract i>In situ hybridization is a powerful tool for the detection and localization of nucleic acid sequences in cells and tissue sections. In this way, the presence of specific DNA fragments as well as (messenger) RNA products can be demonstrated with preservation of morphology (1-3). As morphology may be impaired during in situ hybridization, additional information regarding the cells involved may be required, depending on the scientific or diagnostic problem being studied. Since a particular cellular phenotype is characterized by the presence of specific proteins or antigens, immu
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Carvajal-Valdecasas, Jaime. "DNA radiolabelling and detection." In Gene Probes 1. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780199634019.003.0003.

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Abstract The hybridization process, whereby a target nucleotide sequence can be detected by the complementary binding of a labelled nucleic acid (DNA or RNA probe) is one of the most fundamental techniques used in molecular biology. Based on the procedure originally described by Southern (1), electro phoretic separation of DNA according to molecular weight, usually after endonuclease restriction, is followed by transfer and immobilization on a solid support such as a cellulose membrane. The target sequence is detected by hybridization (2) with a radiolabelled probe and subsequently visualized
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Puvion-Dutilleul, Francine. "Electron microscope in situ hybridization: the non-isotopic post-embedding procedure." In In Situ Hybridization. Oxford University PressOxford, 1998. http://dx.doi.org/10.1093/oso/9780199636594.003.0006.

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Abstract Although the advent of in situ hybridization for light microscopy goes back almost three decades (1, 2) and its extention to the electron microscope (EM) level occurred shortly after, it is only recently that this technique revolution¬ ized our knowledge about the spatial distribution of defined nucleic acid molecules in the cell. Binder et al. (3) broke new ground in this field by con¬ comitantly using for the first time hydrosoluble resin-embedded material, non-isotopic probes, and immunogold labelling of hybrids. This post embedding non-radioactive procedure was immediately adopted
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