Log in

Relevant bibliographies by topics / Non-radioactive molecular hybridization / Journal articles

To see the other types of publications on this topic, follow the link: Non-radioactive molecular hybridization.

Journal articles on the topic 'Non-radioactive molecular hybridization'

Author: Grafiati

Published: 5 June 2025

Last updated: 16 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Non-radioactive molecular hybridization.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Cremers, A. F. M., N. Jansen in de Wal, J. Wiegant, et al. "Non-radioactive in situ hybridization." Histochemistry 86, no. 6 (1987): 609–15. http://dx.doi.org/10.1007/bf00489555.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Durrant, Ian. "Fluorescent Molecules as Direct and Indirect Labels for in situ Hybridization." Proceedings, annual meeting, Electron Microscopy Society of America 54 (August 11, 1996): 24–25. http://dx.doi.org/10.1017/s0424820100162582.

Full text

Abstract:

In situ hybridization has traditionally been performed with radioactive probes. Over recent years effort has been spent in elucidating non-radioactive alternatives for a variety of molecular biology systems. Non-radioactive in situ hybridization is possible, but to date the systems available have, in general, delivered lower sensitivity and higher backgrounds than traditional methods. We have studied a variety of alternative labelling and detection processes; different systems may be required for different applications.Fluorescein has been developed as a new label for all aspects of non-radioactive in situ hybridization. Separate systems have been investigated for DNA, RNA and oligonucleotide probes (see Figure 1). These probes can be applied to frozen sections, paraffin sections, whole mounts, cells and chromosomes. The standard non-radioactive detection system utilizes alkaline phosphatase as an end point to produce a coloured reaction product precipitated at the point of hybridization. This gives both good resolution and sensitivity. Other options becoming available, based on different enzymes and other formats of detection, include peroxidase and gold in conjunction with colour, chemiluminescence and direct and indirect fluorescence. These alternatives have been investigated using the fluorescein based probe labelling systems.

APA, Harvard, Vancouver, ISO, and other styles

3

Wiegant, J. C. A. G., A. K. Raap, and M. van der Ploeg. "7 Methods for non-radioactive in situ hybridization." Cancer Genetics and Cytogenetics 38, no. 2 (1989): 155. http://dx.doi.org/10.1016/0165-4608(89)90535-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

KUMMERT, J., D. COLINET, and P. LEPOIVRE. "Detection of plant viruses by molecular hybridization using non-radioactive probes." EPPO Bulletin 25, no. 1-2 (1995): 301–13. http://dx.doi.org/10.1111/j.1365-2338.1995.tb01471.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Khan, S. A., M. S. Nawaz, A. A. Khan, and C. E. Cerniglia. "Direct in-gel hybridization of digoxigenin-labelled non-radioactive probes." Molecular and Cellular Probes 13, no. 3 (1999): 233–37. http://dx.doi.org/10.1006/mcpr.1999.0241.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Quibriac, M., J. Petitjean, V. Thiers, P. Tiollais, C. Brechot, and F. Freymuth. "Comparison of a non-radioactive hybridization assay for detection of hepatitis B virus DNA with the radioactive method." Molecular and Cellular Probes 3, no. 3 (1989): 209–12. http://dx.doi.org/10.1016/0890-8508(89)90001-7.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Asinari, F., E. E. Cafrune, F. A. Guzman, L. R. Conci, and V. C. Conci. "Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry." AgriScientia 33, no. 1 (2016): 39–45. http://dx.doi.org/10.31047/1668.298x.v33.n1.16570.

Full text

Abstract:

The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3'UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA extraction protocols were evaluated, and the modified cetyltrimethylammonium bromide (CTAB) method showed the highest sensitivity level. Leaves at different phenological stages and petioles were evaluated; the highest reaction was observed in old leaves and in petioles.

APA, Harvard, Vancouver, ISO, and other styles

8

Bochenek, Birgit, and Ann M. Hirsch. "In-situ hybridization of nodulin mRNAs in root nodules using non-radioactive probes." Plant Molecular Biology Reporter 9, no. 3 (1991): 219. http://dx.doi.org/10.1007/bf02672070.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Bochenek, Birgit, and Ann M. Hirsch. "In-Situ hybridization of nodulin mRNAs in root nodules using non-radioactive probes." Plant Molecular Biology Reporter 8, no. 4 (1990): 237–48. http://dx.doi.org/10.1007/bf02668761.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Larsson, L. I., and D. M. Hougaard. "Optimization of non-radioactive in situ hybridization: image analysis of varying pretreatment, hybridization and probe labelling conditions." Histochemistry 93, no. 4 (1990): 347–54. http://dx.doi.org/10.1007/bf00315849.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Lazarov, Nikolai E., Ulrike Schmidt, Ina Wanner, and C. Pilgrim. "Mapping of D 1 dopamine receptor mRNA by non-radioactive in situ hybridization." Histochemistry and Cell Biology 109, no. 3 (1998): 271–79. http://dx.doi.org/10.1007/s004180050227.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Donovan, Richard M., Charlene E. Bush, William R. Peterson, et al. "Comparison of non-radioactive DNA hybridization probes to detect human immunodeficiency virus nucleic acid." Molecular and Cellular Probes 1, no. 4 (1987): 359–66. http://dx.doi.org/10.1016/0890-8508(87)90017-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Paranavitana, C. M. "Non-radioactive detection of K-rasmutations by nested allele specific PCR and oligonucleotide hybridization." Molecular and Cellular Probes 12, no. 5 (1998): 309–15. http://dx.doi.org/10.1006/mcpr.1998.0185.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Olenik, Claudia, and Dieter K. Meyer. "Development of proenkephalin gene expression in rat neocortex: A non-radioactive in situ hybridization study." Molecular Brain Research 44, no. 1 (1997): 83–91. http://dx.doi.org/10.1016/s0169-328x(96)00190-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Fajardo, Thor Vinícius Martins, and Osmar Nickel. "Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe." Ciência Rural 44, no. 10 (2014): 1711–14. http://dx.doi.org/10.1590/0103-8478cr20131629.

Full text

Abstract:

The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was sufficiently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specificity was confirmed by reaction with homologous viral cDNA, individually cloned for each virus.

APA, Harvard, Vancouver, ISO, and other styles

16

Hopman, A. H. N., J. Wiegant, A. K. Raap, J. E. Landegent, M. van der Ploeg, and P. van Duijn. "Bi-color detection of two target DNAs by non-radioactive in situ hybridization." Histochemistry 85, no. 1 (1986): 1–4. http://dx.doi.org/10.1007/bf00508646.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Multhaupt, H., G. Gross, P. Fritz, and K. Kohler. "Cellular localization of induced human interferon-? mRNA by non-radioactive in situ hybridization." Histochemistry 91, no. 4 (1989): 315–19. http://dx.doi.org/10.1007/bf00493007.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Chevrier, Danièle, Søren Richard Rasmussen, and Jean-Luc Guesdon. "PCR product quantification by non-radioactive hybridization procedures using an oligonucleotide covalently bound to microwells." Molecular and Cellular Probes 7, no. 3 (1993): 187–97. http://dx.doi.org/10.1006/mcpr.1993.1028.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Viegas-Péquignot, E., M. Jeanpierre, B. Dutrillaux, F. Apiou, H. Magdelenat, and M. Coppey-Moisan. "9 In situ hybridization of non-radioactive repeated and unique sequences: Application to cancer cytogenetics." Cancer Genetics and Cytogenetics 38, no. 2 (1989): 155. http://dx.doi.org/10.1016/0165-4608(89)90537-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Woodroofe, M. N., and M. L. Cuzner. "Cytokine mRNA expression in inflammatory multiple sclerosis lesions: Detection by non-radioactive in situ hybridization." Cytokine 5, no. 6 (1993): 583–88. http://dx.doi.org/10.1016/s1043-4666(05)80008-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Hougaard, D. M., Henrik Hansen, and Lars-Inge Larsson. "Non-radioactive in situ hybridization for mRNA with emphasis on the use of oligodeoxynucleotide probes." Histochemistry and Cell Biology 108, no. 4-5 (1997): 335–44. http://dx.doi.org/10.1007/s004180050174.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

McRobert, Tracey L., Christina Rudduck, Ursula R. Kees, and O. Margaret Garson. "Detection of MYCN amplification in three neuroblastoma cell lines by non-radioactive chromosomal in situ hybridization." Cancer Genetics and Cytogenetics 59, no. 2 (1992): 128–34. http://dx.doi.org/10.1016/0165-4608(92)90206-n.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Hayashi, Mirian A. F., Raquel S. Pires, Nancy A. Rebouças, Luiz R. G. Britto, and Antonio C. M. Camargo. "Expression of endo-oligopeptidase A in the rat central nervous system: a non-radioactive in situ hybridization study." Molecular Brain Research 89, no. 1-2 (2001): 86–93. http://dx.doi.org/10.1016/s0169-328x(01)00066-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Baumgart, E., Arno Schad, Alfred Völkl, and H. Dariush Fahimi. "Detection of mRNAs encoding peroxisomal proteins by non-radioactive in situ hybridization with digoxigenin-labelled cRNAs." Histochemistry and Cell Biology 108, no. 4-5 (1997): 371–79. http://dx.doi.org/10.1007/s004180050178.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Grudzińska, M., and E. Solarska. "COMPARISON OF NON-RADIOACTIVE MOLECULAR HYBRIDIZATION METHODS AND RT-PCR FOR THE DETECTION OF HOP LATENT VIROID ON HOPS." Acta Horticulturae, no. 656 (September 2004): 187–91. http://dx.doi.org/10.17660/actahortic.2004.656.30.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Giwercman, A., AHN Hopman, FCS Ramaekers, and NE Skakkebæk. "Detection of malignant germ cells in seminal fluid by means of non-radioactive DNA in situ hybridization." Cancer Genetics and Cytogenetics 52, no. 2 (1991): 271. http://dx.doi.org/10.1016/0165-4608(91)90569-g.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Hemmati-Brivanlou, A., D. Frank, M. E. Bolce, B. D. Brown, H. L. Sive, and R. M. Harland. "Localization of specific mRNAs in Xenopus embryos by whole-mount in situ hybridization." Development 110, no. 2 (1990): 325–30. http://dx.doi.org/10.1242/dev.110.2.325.

Full text

Abstract:

We have adapted a non-radioactive technique to detect localized mRNAs in whole-mount Xenopus embryos. Synthetic antisense RNA transcribed in the presence of digoxygenin-UTP is used as a probe and is detected via an anti-digoxygenin antibody. We show that localized mRNAs can be detected from late gastrula to tadpole stages and that high as well as low abundance RNAs can be detected. The method was tested on muscle actin and alpha-globin RNAs, whose localization has previously been characterized. In addition, we used the method to determine the distribution of XA-1 RNA, an anterior ectoderm-specific RNA, which we show is expressed in the periphery of the cement gland as well as in the region of the hatching gland. The sequence of an XA-1 cDNA predicts a protein rich in proline and histidine.

APA, Harvard, Vancouver, ISO, and other styles

28

Kishimoto, J., H. Cox, E. B. Keverne, and P. C. Emson. "Cellular localization of putative odorant receptor mRNAs in olfactory and chemosensory neurons: a non radioactive in situ hybridization study." Molecular Brain Research 23, no. 1-2 (1994): 33–39. http://dx.doi.org/10.1016/0169-328x(94)90208-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Kiyama, Hiroshi, Piers C. Emson, and Masaya Tohyama. "Recent progress in the use of the technique of non-radioactive in situ hybridization histochemistry: new tools for molecular neurobiology." Neuroscience Research 9, no. 1 (1990): 1–21. http://dx.doi.org/10.1016/0168-0102(90)90041-c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

SÁNCHEZ‐NAVARRO, J. A., E. A. CANO, and V. PALLÁS. "Non‐radioactive molecular hybridization detection of carnation mottle virus in infected carnations and its comparison to serological and biological techniques." Plant Pathology 45, no. 2 (1996): 375–82. http://dx.doi.org/10.1046/j.1365-3059.1996.d01-1.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Sakamoto, Hiroshi, François Traincard, Tuyen Vo-Quang, Thérèse Ternynck, Jean-Luc Guesdon, and Stratis Avrameas. "5-Bromodeoxyuridine in vivo labelling of M13 DNA, and its use as a non-radioactive probe for hybridization experiments." Molecular and Cellular Probes 1, no. 1 (1987): 109–20. http://dx.doi.org/10.1016/0890-8508(87)90011-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Shibata, Y., S. Fujita, H. Takahashi, A. Yamaguchi, and T. Koji. "Assessment of decalcifying protocols for detection of specific RNA by non-radioactive in situ hybridization in calcified tissues." Histochemistry and Cell Biology 113, no. 3 (2000): 153–59. http://dx.doi.org/10.1007/s004180050434.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Mullink, H., J. M. M. Walboomers, A. K. Raap, and C. J. L. M. Meyer. "Two colour DNA in situ hybridization for the detection of two viral genomes using non-radioactive probes." Histochemistry 91, no. 3 (1989): 195–98. http://dx.doi.org/10.1007/bf00490132.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Kiyama, Hiroshi, Eileen M. Mc Gowan, and Piers C. Emson. "Co-expression of cholecystokinin mRNA and tyrosine hydroxylase mRNA in populations of rat substantia nigra cells; a study using a combined radioactive and non-radioactive in situ hybridization procedure." Molecular Brain Research 9, no. 1-2 (1991): 87–93. http://dx.doi.org/10.1016/0169-328x(91)90133-i.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Baumgartner, M., E. Viegas-Pequignot, F. Hoffschir, M. Ricoul, A. Bravard, and B. Dutrillaux. "Detection of translocations of 10p by non-radioactive in situ hybridization of VIM gene in SV40-transformed human cell lines." Cancer Genetics and Cytogenetics 56, no. 1 (1991): 23–29. http://dx.doi.org/10.1016/0165-4608(91)90358-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Suijkerbuijk, R., B. de Leeuw, B. de Jong, et al. "Title: Application of non-radioactive in situ hybridization in the detection of chromosome 12 aberrations in human germ cell tumors." Cancer Genetics and Cytogenetics 52, no. 2 (1991): 279. http://dx.doi.org/10.1016/0165-4608(91)90583-g.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Sorg, U. R., J. Enczmann, R. V. Sorg, and P. Wernet. "ONE-STEP MOLECULAR HLA-DR PRESCREENING EMPLOYING A SET OF 14 SEQUENCE SPECIFIC OLIGONUCLEOTIDES IN A NON-RADIOACTIVE TETRAMETHYLAMMONIUM CHLORIDE HYBRIDIZATION PROTOCOL." European Journal of Immunogenetics 19, no. 6 (1992): 391–401. http://dx.doi.org/10.1111/j.1744-313x.1992.tb00082.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Casadémont, I., C. Bizet, D. Chevrier, and J. L. Guesdon. "Rapid detection of Campylobacter fetus by polymerase chain reaction combined with non-radioactive hybridization using an oligonucleotide covalently bound to microwells." Molecular and Cellular Probes 14, no. 4 (2000): 233–40. http://dx.doi.org/10.1006/mcpr.2000.0312.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Ichimiya, Yosuke, Piers C. Emson, Chris Christodoulou, Michael J. Gait, and Jerry L. Ruth. "Simultaneous Visualization of Vasopressin and Oxytocin mRNA- Containing Neurons in the Hypothalamus Using Non-Radioactive in situ Hybridization Histochemistry." Journal of Neuroendocrinology 1, no. 2 (1989): 73–75. http://dx.doi.org/10.1111/j.1365-2826.1989.tb00081.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Haupt, Corinna, Else A. Tolner, Uwe Heinemann, Otto W. Witte, and Christiane Frahm. "The combined use of non-radioactive in situ hybridization and real-time RT-PCR to assess gene expression in cryosections." Brain Research 1118, no. 1 (2006): 232–38. http://dx.doi.org/10.1016/j.brainres.2006.08.037.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Zamora-Macorra, Erika Janet, Daniel Leobardo Ochoa-Martínez, Guadalupe Valdovinos-Ponce, et al. "Simultaneous detection of Clavibacter michiganensis subsp. michiganensis, Pepino mosaic virus and Mexican papita viroid by non-radioactive molecular hybridization using a unique polyprobe." European Journal of Plant Pathology 143, no. 4 (2015): 779–87. http://dx.doi.org/10.1007/s10658-015-0729-1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Speel, E. J., B. Schutte, F. C. Ramaekers, and A. H. Hopman. "The effect of avidin-biotin interactions in detection systems for in situ hybridization." Journal of Histochemistry & Cytochemistry 40, no. 1 (1992): 135–41. http://dx.doi.org/10.1177/40.1.1729352.

Full text

Abstract:

The effect of avidin-biotin interactions in several detection systems for the non-radioactive in situ hybridization (ISH) technique was studied in a model system using a transitional cell carcinoma line and a biotinylated DNA probe. We performed fluorescence ISH to unravel the individual steps in a sensitive and frequently used amplification method which makes use of the alternating cytochemical detection layers of fluorescein isothiocyanate-conjugated avidin (AvFITC) and biotinylated goat anti-avidin (BioGAA) antibodies to detect the hybridized and biotinylated probe. Our experiments revealed that BioGAA antibodies bind with their antigen binding sites and not with their biotin moieties to avidin molecules that have already interacted with the DNA probe. The probable working mechanism of this amplification method is presented in a model. Furthermore, we used a peroxidase staining technique to compare with each other the sensitivity of several other detection systems in which avidin-biotin interactions play an important role, e.g., the avidin-biotinylated peroxidase complex (ABC) system. The experiments show that avidin molecules can not be efficiently used to interconnect two biotinylated molecular layers, since their introduction leads to firmly closed cytochemical networks. Such a closed network is already formed between the hybridized and biotinylated DNA probe and a first detection layer of avidin molecules, as appears from the finding that biotinylated molecules could hardly be coupled to these avidin molecules in a following detection layer. Therefore, the results presented here provide us with new insight into the molecular basis of cytochemical network formation. This will enable us to choose the proper procedures for increasing the sensitivity of ISH detection systems.

APA, Harvard, Vancouver, ISO, and other styles

43

Larsson, L. I., and D. M. Hougaard. "Detection of gastrin and its messenger RNA in Zollinger-Ellison tumors by non-radioactive in situ hybridization and immunocytochemistry." Histochemistry 97, no. 2 (1992): 105–10. http://dx.doi.org/10.1007/bf00267299.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

KOCAN, KATHERINE M., NIE-LIN GE, EDMOUR F. BLOUIN, and GEORGE L. MURPHY. "Development of a Non-radioactive DNA Probe and in Situ Hybridization for Detection of Anaplasma marginale in Ticks and Cattlea." Annals of the New York Academy of Sciences 849, no. 1 (1998): 137–45. http://dx.doi.org/10.1111/j.1749-6632.1998.tb11042.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Heppelmann, Bernd, and Piers C. Emson. "Distribution of calretinin mRNA in rat spinal cord and dorsal root ganglia: a study using non-radioactive in situ hybridization histochemistry." Brain Research 624, no. 1-2 (1993): 312–16. http://dx.doi.org/10.1016/0006-8993(93)90095-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Bradford, Debbie, Camilla Christensson, Nicole Jakab, and Linda L. Blackall. "Molecular biological methods to detect “Microthrix parvicella” and to determine its abundance in activated sludge." Water Science and Technology 37, no. 4-5 (1998): 37–45. http://dx.doi.org/10.2166/wst.1998.0575.

Full text

Abstract:

Molecular biological methods were evaluated in attempts to detect and quantitate levels of “Microthrix parvicella” in activated sludges. Approximately 66% of the 23S rRNA gene sequence of a strain (Ben43) of the Gram positive bulking and foaming organism “Microthrix parvicella” was determined, while a lesser amount was determined for “M. parvicella” strain RN1. The high mol%G+C Gram positive bacteria (HGCGPBs) possess two powerfully diagnostic regions in the 23S rDNA and these were investigated in both strains. Firstly, the 18 nucleotide HGCGPB probe sequence (HGC69a) varied in at least two nucleotides with the sequence from both strains of “M. parvicella”. Secondly, an approximately 100 nucleotide stable insert between helices 54 and 55 in the 23S rRNA of HGCGPBs was discovered to be present in “M. parvicella”, but in both strains it was unique in length (79 nucleotides) and sequence. The region of the 23S rDNA with the stable insert was exploited to develop a polymerase chain reaction assay in which amplicons from “M. parvicella” were larger than those from nonHGCGPBs (i.e. all Bacteria except the HGCGPBs), and smaller than those from HGCGPBs. This assay was evaluated with DNAs extracted from activated sludges but although “M. parvicella” was morphologically identified, and was a dominant filament in at least one of the samples, no “M. parvicella” specific sized amplicons could be recovered from it. Amplicons of sizes generated by nonHGCGPBs and HGCGPBs were routinely produced in the stable insert PCR with DNAs from activated sludges where the highest yield was of amplicons from nonHGCGPBs. A second series of experiments were undertaken with the objective of evaluating the use of a non-radioactive hybridization method, based on extraction of bacterial RNA, for quantifying “M. parvicella” in activated sludge samples. Total nucleic acids were extracted from activated sludge samples and immobilized on nylon membranes. Probing with 16S rRNA-directed DIG-labelled oligonucleotide probes, detection of chemiluminescent signals on membranes and densitometry allowed hybridization signals to be quantified. The relationship between the amount of nucleic acid hybridized and the hybridization signal intensity observed was found to be linear over a specified range of signal intensities. A range of activated sludge samples were analysed for “M. parvicella” and variations in levels could be distinguished.

APA, Harvard, Vancouver, ISO, and other styles

47

FERNANDEZ-PRADA, C. M., M. M. VENKATESAN, A. A. FRANCO, et al. "Molecular epidemiology of Shigella flexneri in a diarrhoea-endemic area of Lima, Peru." Epidemiology and Infection 132, no. 2 (2004): 303–16. http://dx.doi.org/10.1017/s0950268803001560.

Full text

Abstract:

A year-long community-based study of diarrhoeal diseases was conducted in Canto Grande, a periurban community in Lima, Peru. In 109 (34%) houses out of 323 that were visited, at least one individual was detected with shigellosis. The frequency of the 161 shigella isolates obtained was as follows: 117 S. flexneri (73%), 21 S. boydii (13%), 15 S. dysenteriae (9%), and 8 S. sonnei (5%). Using a non-radioactive ipaH gene probe as a molecular epidemiological tool, a total of 41 S. flexneri strains were shown to be distributed in 25 intra-family comparisons by pairs (icp). Further subdivision, based on a comparison of the serotype, plasmid profile, antibiotic resistances and ipaH hybridization patterns indicated that Group I, with 11 icp (44%), had strains that were identical, Group II with 8 icp (32%), had strains that were different and Group III with 6 icp (24%), had strains with the same serotype and identical ipaH profiles but with differences in other markers. This data indicates that a diversity of shigella clones circulated in this community resulting from both clonal spread and horizontal transfer of genetic elements. Furthermore, ipaH profiling of isolates can be used not only to differentiate between closely related shigella strains but also with other parameters, help to understand the dynamics of the generation of new clones of pathogenic bacteria.

APA, Harvard, Vancouver, ISO, and other styles

48

Umesha, D., P. P. Srivastava, and S. D. Singh. "Molecular Characterization in Tor khudree Using Dot-Blot Analysis and Cross Hybridization Through Species-Specificity of the Non-Radioactive Digoxigenin (DIG) Labeled Specific DNA Probe." National Academy Science Letters 35, no. 1 (2012): 19–26. http://dx.doi.org/10.1007/s40009-011-0004-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Voliva, C. F., and K. Paigen. "Isolation of the mouse cytochrome P450J (CYP2E1) cDNA and its reciprocal testosterone regulation in kidney and liver." Journal of Molecular Endocrinology 7, no. 2 (1991): 155–66. http://dx.doi.org/10.1677/jme.0.0070155.

Full text

Abstract:

ABSTRACT A hybridization probe that is homologous to the B2 short interspersed repetitive element detects an mRNA in mouse kidney and liver that is regulated by androgen. Administration of testosterone induces this mRNA in kidney and represses it in liver. The mRNA was cloned by first using the B2 probe to select 48 cDNA clones from an androgen-induced kidney library. These clones were then tested for their androgen response by hybridizing them with probes made by reverse transcription of basal and testosterone-treated kidney poly(A)+ RNA. Any homology to the B2 sequence was masked by prehybridizing the filters to an excess of non-radioactive RNA synthesized from a B2 sequence cloned into a riboprobe vector. A unique sequence was subcloned from the largest androgen-responsive cDNA clone. A radioactive riboprobe generated from the unique sequence subclone detected an androgen-responsive mRNA in Northern blots with the same electrophoretic mobility as the predominant androgen-responsive mRNA detected with the B2 homologous riboprobe. The riboprobe also detected a unique sequence in Southern blots of genomic DNA. This subclone was then used as the probe to isolate a full-length cDNA clone from a second androgen-induced kidney library. When sequenced, this full-length cDNA of an androgen-responsive, B2-containing mRNA showed strong homology to the rat and human cytochrome P450J and the rabbit cytochrome P450 3a genes (CYP2E1). It showed only weak homology to the mouse testosterone 15 α-hydroxylase gene (CYP2A3) which is also regulated reciprocally by androgen in kidney and liver. The sequence of mouse P450J is identical to the B2 homologous mRNA previously named B2+mRNAx which is abundant in mouse liver.

APA, Harvard, Vancouver, ISO, and other styles

50

Foster, J. A., S. J. Rush, and I. R. Brown. "Localization of constitutive and hyperthermia-inducible heat shock mRNAS (hsc70 and hsp70) in the rabbit cerebellum and brainstem by non-radioactive in situ hybridization." Journal of Neuroscience Research 41, no. 5 (1995): 603–12. http://dx.doi.org/10.1002/jnr.490410506.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!