Relevant bibliographies by topics / Non-specific binding

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Non-specific binding'

Author: Grafiati
Published: 4 June 2021
Last updated: 28 January 2023

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Journal articles on the topic "Non-specific binding"

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Flintoft, Louisa. "Surveying non-specific binding." Nature Reviews Genetics 14, no. 10 (September 18, 2013): 676. http://dx.doi.org/10.1038/nrg3586.

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Chen, Shi-Jie. "Site-Specific Binding of Non-Site-Specific Ions." Biophysical Journal 116, no. 12 (June 2019): 2237–39. http://dx.doi.org/10.1016/j.bpj.2019.04.038.

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Zenei, Taira, and Terada Hiroshi. "Specific and non-specific ligand binding to serum albumin." Biochemical Pharmacology 34, no. 11 (June 1985): 1999–2005. http://dx.doi.org/10.1016/0006-2952(85)90322-3.

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Shimizu, Seishi, Steven Abbott, Katarzyna Adamska, and Adam Voelkel. "Quantifying non-specific interactionsvialiquid chromatography." Analyst 144, no. 5 (2019): 1632–41. http://dx.doi.org/10.1039/c8an02244e.

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Mendel, C. M., and D. B. Mendel. "‘Non-specific’ binding. The problem, and a solution." Biochemical Journal 228, no. 1 (May 15, 1985): 269–72. http://dx.doi.org/10.1042/bj2280269.

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The concept of ‘non-specific’ binding, as it relates to studies of the binding of hormones to their receptors, is reviewed. It is concluded that the most widely used operational definition, namely binding that is not displaceable by an excess of unlabelled ligand, is often inaccurate, resulting either in overestimation of the number of high-affinity receptors and underestimation of the affinity of a given hormone for its receptor, or in a curvilinear Scatchard plot suggesting (artifactually) the presence of negative co-operativity or multiple classes of binding sites. The general use of an alt
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Oda, Masayuki, Koji Furukawa, Kazuhiro Ogata, Akinori Sarai, and Haruki Nakamura. "Thermodynamics of specific and non-specific DNA binding by the c-myb DNA-binding domain." Journal of Molecular Biology 276, no. 3 (February 1998): 571–90. http://dx.doi.org/10.1006/jmbi.1997.1564.

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de Bruijn, Peter, Inge M. Ghobadi Moghaddam-Helmantel, Walter J. Loos, Ron H. J. Mathijssen, and Erik A. C. Wiemer. "The issue of non-specific binding of cabazitaxel." Journal of Chromatography B 932 (August 2013): 74–75. http://dx.doi.org/10.1016/j.jchromb.2013.06.016.

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Taylor, Thomas J., Gerald W. Parker, Abram B. Fajer, and Kay A. Mereish. "Non-specific binding of palytoxin to plastic surfaces." Toxicology Letters 57, no. 3 (August 1991): 291–96. http://dx.doi.org/10.1016/0378-4274(91)90203-i.

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Smith, Phil M., Indorica Sutradhar, Maxwell Telmer, Rishikesh Magar, Amir Barati Farimani, and B. Reeja-Jayan. "Isolating Specific vs. Non-Specific Binding Responses in Conducting Polymer Biosensors for Bio-Fingerprinting." Sensors 21, no. 19 (September 22, 2021): 6335. http://dx.doi.org/10.3390/s21196335.

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A longstanding challenge for accurate sensing of biomolecules such as proteins concerns specifically detecting a target analyte in a complex sample (e.g., food) without suffering from nonspecific binding or interactions from the target itself or other analytes present in the sample. Every sensor suffers from this fundamental drawback, which limits its sensitivity, specificity, and longevity. Existing efforts to improve signal-to-noise ratio involve introducing additional steps to reduce nonspecific binding, which increases the cost of the sensor. Conducting polymer-based chemiresistive biosens
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Fowers, K. D., and J. Kopeček. "Development of a fibrinolytic surface: specific and non-specific binding of plasminogen." Colloids and Surfaces B: Biointerfaces 9, no. 6 (September 1997): 315–30. http://dx.doi.org/10.1016/s0927-7765(97)00034-9.

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Dissertations / Theses on the topic "Non-specific binding"

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Shedge, Hemangi Y. "Specific and non-specific binding of proteins and nucleic acids on chemically modified reticulated vitreous carbon electrodes." Connect to this title online, 2009. http://etd.lib.clemson.edu/documents/1246559605/.

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McLure, James Alexander, and james mclure@flinders edu au. "Physicochemical determinants of the non-specific binding of drugs to human liver microsomes." Flinders University. Medicine, 2008. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20081102.165952.

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Accurate determination of the in vitro kinetic parameters Km (Michaelis constant) and Ki (inhibition constant) is critical for the quantitative prediction of in vivo drug clearance and the magnitude of inhibitory drug interactions. A cause of inaccuracy in vitro arises from the assumption that all drug added to an incubation mixture is available for metabolism or inhibition. Many drugs bind non-specifically to the membrane of the in vitro enzyme source. The aims of this thesis were to: 1) investigate the comparative importance of lipophilicity (as log P), and pKa as determinants of the non-sp
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Slowski, Kathryn Johanna. "Identification of a novel microRNA involved in non-specific binding to a decoy transcript." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/59879.

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MicroRNAs are known to be upregulated or downregulated in various types of cancer, leading to changes in the expression of genes involved in cellular proliferation, anti-apoptosis, migration, and invasion. To study the effects of microRNA loss or gain in different neoplasms, numerous models have been described to decrease or increase expression of microRNAs, but the off-target effects of different methods have not been well investigated. I investigated the possibility of off-target effects in a model of miR-143 knockdown in myeloid leukemia cell lines that implemented a microRNA sponge, or dec
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Child, Chloe Rose. "Determing structure-activity relationships between novel PET radiotracers and their non-specific binding properties." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9576.

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The non-invasive imaging modality positron emission tomography (PET) is used extensively in clinical settings and is increasingly being used by the pharmaceutical industry in drug development. Molecules of biological interest are labelled with positron emitting isotopes e.g. 11C, allowing their biodistribution and kinetics to be followed in vivo. A major factor in the failure of radioligands is the magnitude of unwanted background signal, non-specific binding (NSB) obscuring binding to the desired target. Assumptions have previously been made as to the physiochemical and pharmacological proper
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Westerhult, David. "Weak Affinity Chromatography : Evaluation of Different Silica Supports for Protein Immobilizationand Effect of Mobile Phases Regarding Retentionand Non-specific Binding." Thesis, Linnéuniversitetet, Institutionen för naturvetenskap, NV, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-17968.

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Fragment based lead discovery (FBLD), where libraries of small fragments are screened andlater on developed to lead compounds, is an alternative to the classical drug discovery methods such as high trough-put screening. Weak affinity chromatography (WAC) is a new promising approach to the screening process of FBLD. WAC is performed by injections of fragments onto a high performance liquid chromatography (HPLC) column in which a protein is immobilized to a silica support. The retention of the injected fragments is correlated to the binding affinity of the fragments towards the immobilized prote
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Fouquier, d'Herouel Aymeric. "Statistical models of TF/DNA interaction." Licentiate thesis, Stockholm : Numerisk analys och datalogi, Numerical Analysis and Computer Science, Kungliga Tekniska högskolan, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4633.

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Cowsill, Benjamin James. "The physics of pregnancy tests : a biophysical study of interfacial protein adsorption." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/the-physics-of-pregnancy-tests-a-biophysical-study-of-interfacial-protein-adsorption(538b8e9c-9111-4eb9-ac7d-e5e75110e315).html.

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Pregnancy tests and related immunoassays are heavily dependent on specific and non-specific protein adsorption. These interfacial processes are affected by many factors that influence the in situ conformations of interfacially immobilised antibodies. This thesis examines a number of representative features with dual polarisation interferometry (DPI) and neutron reflection (NR), thus combining real-time dynamic monitoring with high interfacial structural resolution. Bovine serum albumin (BSA) was initially used as a model system to compare the surface coverage and thickness measurements of DPI
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Richardson, Mandek. "Theoretical and Experimental Investigations to Improve the Performance of Surface Acoustic Wave (SAW) Biosensors." Scholar Commons, 2014. https://scholarcommons.usf.edu/etd/5566.

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The objective of this dissertation is to improve the performance of surface acoustic wave (SAW) biosensors for use in point-of-care-testing (POCT) applications. SAW biosensors have the ability to perform fast, accurate detection of an analyte in real time without the use of labels. However, the technology suffers from the inability to differentiate between specific and non-specific binding. Due to this limitation, direct testing of bodily fluids using SAW sensors to accurately determine an analyte's concentration is difficult. In addition, these sensors are challenged by the need to detect sma
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Vasquez, Colins. "Allosteric effects of the GTP-specific binding site and a benzimidazole-derivative non-nucleoside inhibitor on the hepatitis C virus RNA-dependent RNA polymerase." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40750.

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The hepatitis C virus (HCV) is a positive-strand RNA virus that belongs to the Flaviviridae family. HCV expresses the non-structural protein, NS5B, an RNA-dependent RNA polymerase required for genome replication. Here, we describe the allosteric properties of both the GTP-specific binding site (G-site) of the HCV polymerase and VIR-1327, a benzimidazole-derivative non-nucleoside inhibitor (NNI). Using biochemical assays, we identified certain G-site residues that affect NS5B activity, including serine-29, proline-495 and valine-499. We also demonstrated that VIR-1327 is a potent inhibitor with
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Engqvist, Martin. "A generic capture assay for immunogenicity, using Biacore." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-198767.

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The purpose of this investigation was to create and optimise a capture assay for the detectionof anti-drug antibodies (ADA) in human plasma, using Biacore. We also dealt with the nonspecificplasma binding to mouse-derived anti-biotin which may occur in the capture assay.By paying attention to these things we aimed at reaching as high sensitivity as possible for theADA detection. The capture assay also benefited and gained flexibility from using the same regenerationsolution irrespective of drug and from having a composition that minimises the risk ofdamaging drug epitopes.
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Books on the topic "Non-specific binding"

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Jörres, Achim, Dietrich Hasper, and Michael Oppert. Non-dialytic management of the patient with acute kidney injury. Edited by Norbert Lameire. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199592548.003.0228.

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The main focus in the non-dialytic management of patients with acute kidney injury (AKI) is the prevention and treatment of complications.Nutritional support is an important aspect as many patients tend to be hypercatabolic, thus requiring adequate caloric intake, yet without administration of excessive fluid volumes. Inadequate nutrition in AKI may lead to enhanced production of urea nitrogen and azotaemia. However, hyperglycaemia is a frequent complication in these patients, often requiring continuous insulin therapy to achieve the recommended blood glucose target range of 110–150 mg/dL (6.1
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Pauwelyn, Joost. Sources of International Trade Law. Edited by Samantha Besson and Jean d’Aspremont. Oxford University Press, 2018. http://dx.doi.org/10.1093/law/9780198745365.003.0048.

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This chapter argues that the World Trade Organization (WTO) approach to sources of law is legal-positivist, non-teleological, and focused predominantly on the text of WTO covered agreements as explicitly agreed to by WTO members. This approach places heavy reliance on a de facto rule of precedent and an increasing role for non-binding instruments, with little or no reference to academic writings and a limited role for non-WTO rules of international law other than mainly procedural rules of general international law. Moreover, the WTO’s sources doctrine remains relatively traditional or mainstr
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Roy, Goode, Kronke Herbert, and McKendrick Ewan, eds. Part III Harmonization of General Contract Law, 16 Restatements of Contract Law. Oxford University Press, 2015. http://dx.doi.org/10.1093/law/9780198735441.003.0017.

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This chapter examines non-binding restatements of contract law, in particular the UNIDROIT Principles of International Commercial Contracts and the Principles of European Contract Law. It considers the nature, purposes, scope, sphere of application, and substantive content of these Principles (including freedom of contract, pacta sunt servanda, good faith, interpretation, adequate assurance of performance, specific performance, and other remedies and hardship and change of circumstances). The chapter considers the extent to which these Principles can be used in litigation and in arbitration an
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Berger, Tobias. Between the State and the Shalish. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780198807865.003.0004.

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This chapter reconstructs the background knowledge that underpins all processes of conflict resolution in rural Bangladesh. It starts with the in-depth analysis of an attempted murder case and shows how state and non-state courts are intricately intertwined in what is conceptualized as the logic of non-enforcement. According to this logic, people frequently file cases with the state court, but not to get binding and enforceable verdicts. Instead, they do so to alter the dynamics of non-state courts. Although non-state justice institutions respond to representations of the state (for example in
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Papadopoulos, Yannis. Multilevel Governance and Depoliticization. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780198748977.003.0007.

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‘Multilevel’ governance (MLG) refers to the fact that, in contemporary established democracies, collectively binding decisions are frequently formulated or implemented in a cooperative manner by networks composed of public actors attached to different jurisdictional levels (from the local to the supranational) and of non-public actors such as experts, interest representatives, and members of cause groups. This chapter develops the expectation that the occurrence and magnitude of depoliticization in MLG depend on a number of its defining traits, and that the presence and intensity of these trai
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James, Harrison. 1 Introduction. Oxford University Press, 2017. http://dx.doi.org/10.1093/law/9780198707325.003.0001.

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Chapter 1 provides an introduction to both the anthropogenic threats facing the marine environment at the beginning of the twenty-first century and the role that international law plays in regulating humankind’s impacts on the oceans. It argues that the protection of the marine environment is a common concern of humankind, requiring the cooperation of all States in adopting appropriate international rules and standards to address the main threats to the marine environment and collective efforts to ensure the enforcement of those norms. The chapter then explains the key sources of international
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Das, Onita, and Aneaka Kellayv. Private Security Companies and Other Private Security Service Providers (PSCs) and Environmental Protection in Jus Post Bellum. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780198784630.003.0014.

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A challenge to environmental protection and the jus post bellum framework is the rise in Private Security Companies and other Private Security Service Providers (PSCs). The marked increase in the outsourcing of vast amounts of operational and logistical work to PSCs have caused key issues around PSC oversight, regulation, and concern around civilian protection linked to environmental issues to arise. Using the Iraq (2003–11) and Afghanistan (2001–14) conflicts as examples, this chapter explores the growth of PSCs, their environmental performance, and reviews the adequacy of legal and policy fr
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Kohn, Robert. Human Rights and the Elderly. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780199374656.003.0042.

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Attention to the human rights of the elderly has gained increased international attention. This chapter provides an overview of some international agencies’ efforts to address human rights of elders, as well as related efforts in different regions of the world. The UN does not have a specific treaty or convention regarding human rights of the elderly; only in 1995 was comprehensive legal analysis of the rights of the elderly addressed. The 2002 Madrid International Action Plan on Aging was a non-legally binding declaration by participating governments. Article 14 addresses equal access to heal
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Faarlund, Jan Terje. The Syntax of Mainland Scandinavian. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198817918.001.0001.

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The term Mainland Scandinavian covers the North Germanic languages spoken in Denmark, Norway, Sweden, and parts of Finland. There is a continuum of mutually intelligible standard languages, regional varieties, and dialects stretching from southern Jutland to Eastern Finland. Linguistically, Danish, Norwegian, and Swedish are thus to be considered one language. Most syntactic patterns and features are shared among the national and regional varieties, but there are also interesting differences. This book presents the main syntactic structures of this language, with the focus on the standard lang
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Gill, Steven J., and Michael H. Nathanson. Central nervous system pathologies and anaesthesia. Edited by Philip M. Hopkins. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780199642045.003.0081.

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Anaesthesia induces changes in many organ systems within the body, though clearly none more so than the central nervous system. The physiology of the normal central nervous system is complex and the addition of chronic pathology and polypharmacy creates a significant challenge for the anaesthetist. This chapter demonstrates a common approach for the anaesthetist and specific considerations for a wide range of neurological conditions. Detailed preoperative assessment is essential to gain understanding of the current symptomatology and neurological deficit, including at times restrictions on mov
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Book chapters on the topic "Non-specific binding"

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Meijer, Ellen A., Sacco C. de Vries, Peter Sterk, Dorus W. J. Gadella, Karel W. A. Wirtz, and Theo Hendriks. "Characterization of the non-specific lipid transfer protein EP2 from carrot (Daucus carota L.)." In Cellular Fatty Acid-Binding Proteins II, 159–66. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-3096-1_21.

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Wangkam, Thidarat, and Toemsak Srikhirin. "Design Sensor Chip for Repelling the Non-specific Binding for Its Application in Biomedical Sensor." In IFMBE Proceedings, 857–60. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-02913-9_221.

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Vorwerk, P., K. Mohnike, W. F. Blum, U. Kluba, V. Aumann, and U. Mittler. "Insulin-Like Growth Factors and Their Specific Binding Proteins in Leukemia (ALL) and Non-Hodgkin Lymphoma." In Acute Leukemias VI, 161–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60377-8_28.

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Korth, Ruth-Maria. "Non-Specific PAF Binding to Embryonal F9 Cells and Prostacyclin Synthesis in Human Umbilical Vein Endothelial Cells." In Advances in Experimental Medicine and Biology, 291–95. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-0179-8_46.

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Allfrey, Vincent G., Akira Inoue, Jonathan Karn, Edward M. Johnson, Robert A. Good, and John W. Hadden. "Sequence-Specific Binding of DNA by Non-Histone Proteins and Their Migration from Cytoplasm to Nucleus During Gene Activation." In Novartis Foundation Symposia, 199–228. Chichester, UK: John Wiley & Sons, Ltd, 2008. http://dx.doi.org/10.1002/9780470720103.ch12.

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Levitan, Irena, Sang Joon Ahn, Ibra Fancher, and Avia Rosenhouse-Dantsker. "Physiological Roles and Cholesterol Sensitivity of Endothelial Inwardly-Rectifying K+ Channels: Specific Cholesterol-Protein Interactions Through Non Annular Binding Sites." In Vascular Ion Channels in Physiology and Disease, 327–47. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-29635-7_15.

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Prakash, Om, and Feroz Khan. "CoSSDb: A Database of Co-crystallized Ligand Sub-structures for Anticancer Lead Designing & Optimization." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022), 133–41. Dordrecht: Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_14.

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AbstractThe Discovery of the novel optimized structures of small molecules for selective targeting is one of the challenging tasks in drug designing. Bioisosteres are the key components of the lead compound, which provide hidden power to the compound scaffold for selective targeting. We are presenting a database, named CoSSDb which stands for Co-crystallized Sub-Structure Database. The CoSSDb contains ligand sub-structures as possible bioisosteres. extracted from PDB files, available in Protein Data Bank. Sub-structures were extracted through an algorithm, which utilizes the location of atoms in the 3D domain of the complex ligand & protein. It processes the relative positioning of atoms for demarcation of the influential part of the ligand, which interacts with macromolecule and provides potency to that ligand for binding with a specific binding pocket of the protein. The algorithm was used to extract sub-structures from the ligands co-crystallized with proteins involved in cancer. About 7721 x-ray crystallography PDB files were processed, and 654 non-redundant substructures were identified. These sub-structures will be useful during designing & optimization of novel ligands for selective targets. The database is freely accessible at ‘https://opticket49.wixsite.com/substructdb’.
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Arnoldi, Michele, Giulia Zarantonello, Stefano Espinoza, Stefano Gustincich, Francesca Di Leva, and Marta Biagioli. "Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation." In Methods in Molecular Biology, 63–87. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_4.

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AbstractSINEUP is a new class of long non-coding RNAs (lncRNAs) which contain an inverted Short Interspersed Nuclear Element (SINE) B2 element (invSINEB2) necessary to specifically upregulate target gene translation. Originally identified in the mouseAS-Uchl1 (antisense Ubiquitin carboxyl-terminal esterase L1) locus, natural SINEUP molecules are oriented head to head to their sense protein coding, target gene (Uchl1, in this example). Peculiarly, SINEUP is able to augment, in a specific and controlled way, the expression of the target protein, with no alteration of target mRNA levels. SINEUP is characterized by a modular structure with the Binding Domain (BD) providing specificity to the target transcript and an effector domain (ED)—containing the invSINEB2 element—able to promote the loading to the heavy polysomes of the target mRNA. Since the understanding of its modular structure in the endogenous AS-Uchl1 ncRNA, synthetic SINEUP molecules have been developed by creating a specific BD for the gene of interest and placing it upstream the invSINEB2 ED. Synthetic SINEUP is thus a novel molecular tool that potentially may be used for any industrial or biomedical application to enhance protein production, also as possible therapeutic strategy in haploinsufficiency-driven disorders.Here, we describe a detailed protocol to (1) design a specific BD directed to a gene of interest and (2) assemble and clone it with the ED to obtain a functional SINEUP molecule. Then, we provide guidelines to efficiently deliver SINEUP into mammalian cells and evaluate its ability to effectively upregulate target protein translation.
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Magraw, Kendra. "Trends and ISDS Backlash Related to Non-Disputing Treaty Party Submissions." In Public Actors in International Investment Law, 79–96. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-58916-5_5.

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AbstractSome international investment agreements (IIAs) allow states that are parties to a treaty, but are not party to a specific dispute under that treaty, to intervene on a limited basis in order to make submissions on matters of treaty interpretation. Such mechanisms have proved to be highly valued by treaty parties, as evident by the many recently-concluded IIAs containing increasingly sophisticated non-disputing treaty party (NDTP) provisions. This chapter: (1) provides the background on NDTPs mechanisms, with a focus on the North American Free Trade Agreement (NAFTA) (the first-known IIA to contain such a provision); (2) examines the possible connection between tribunals failing to give due regard to treaty parties’ interpretive positions (again focusing on NAFTA) and the current backlash against investor-state dispute settlement (ISDS); and (3) analyses trends in recently-concluded IIAs. It is argued that the apparent lack of deference given by tribunals to NDTP submissions may be contributing to the current backlash against ISDS, based on two discernible trends: (1) an increase in the number of IIAs containing NDTPs provisions; and (2) provisions that now state that not only are treaty interpretations made by treaty parties binding on tribunals (such provisions also have their genesis in NAFTA), but that, in addition, tribunals’ decisions must be consistent with such agreed interpretations (the latter an innovation of a NAFTA party in 2003). Such trends are also visible at the institutional and multilateral levels, such as the revision of the Arbitration Rules of the International Centre for Settlement of Investment Disputes (ICSID) and the Mauritius Convention on Transparency in ISDS of the United Nations Commission on International Trade Law (UNCITRAL), and show no sign of slowing down.
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Danisi, Carmelo, Moira Dustin, Nuno Ferreira, and Nina Held. "Why Sexual Orientation and Gender Identity Asylum?" In IMISCOE Research Series, 3–21. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-69441-8_1.

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AbstractForced migration – no matter how we wish to define it – has been high in the political agendas and debates across the world for several decades. Forced migrants become claimants of international protection, or ‘asylum claimants’, and then find themselves trapped in convoluted, constraining and highly politicised systems. Often accused of being ‘bogus’ asylum claimants, they are also regularly accused of abusing the hospitality of the host country, violating countries’ borders and territorial sovereignty, and simply seeking economic benefits (Ford 2009; UNHCR 2007). Conversely, asylum legal instruments have been repeatedly criticised for inadequately addressing the rights and needs of asylum claimants, therefore preventing those with legitimate claims from being granted protection. These debates have more recently been rehashed in the context of the negotiations behind the Global Compact for Safe Orderly and Regular Migration, a non-legally binding agreement negotiated under the aegis of the United Nations (UN) and endorsed by the UN General Assembly. In this atmosphere of permanent politicised and humanitarian ‘crisis’ (McAdam 2014), a group warranting specific attention is constituted by those asylum claimants presenting a claim based on their sexual orientation or gender identity (SOGI).
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Conference papers on the topic "Non-specific binding"

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King, Benjamin C., Michael Clark, Thomas Burkhead, Palaniappan Sethu, Shesh Rai, Goetz Kloecker, and Balaji Panchapakesan. "Electrical detection of specific versus non-specific binding events in breast cancer cells." In SPIE NanoScience + Engineering, edited by Hooman Mohseni, Massoud H. Agahi, and Manijeh Razeghi. SPIE, 2012. http://dx.doi.org/10.1117/12.929875.

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Tsukiiwa, Mirano, Hironaga Noguchi, and Yuhei Hayamizu. "Self-assembled peptides for suppression of non-specific binding of biomolecules on graphene." In 2020 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2020. http://dx.doi.org/10.7567/ssdm.2020.g-8-02.

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Zainudin, Nor Syuhada, Nor Azura Malini Ahmad Hambali, Mohamad Halim Abd Wahid, Vithyacharan Retnasamy, and Mukhzeer Mohamad Shahimin. "The study of non-fouling and non-specific cellular binding on functionalized surface for mammalian cell identification and manipulation." In ADVANCED MATERIALS ENGINEERING AND TECHNOLOGY V: International Conference on Advanced Material Engineering and Technology 2016. Author(s), 2017. http://dx.doi.org/10.1063/1.4981840.

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Wang, Qian, Min Zhang, Ling Wang, and Yueqing Gu. "Effective reduction of non-specific binding by bovine serum albumin modified quantum dot surface for cell targeted imaging." In Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), edited by Qingming Luo, Lihong V. Wang, and Valery V. Tuchin. SPIE, 2014. http://dx.doi.org/10.1117/12.2068693.

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Zheng, Xiangjun, Siu Lun Cheung, Lian Wang, Joyce A. Schroeder, Ronald L. Heimark, James C. Baygents, Roberto Guzman, and Yitshak Zohar. "Specific Binding of Cancer Cells Using a Microchamber Array Functionalized With Antibodies." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-13217.

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Specific binding of target suspended metastatic cancer cells to an antibody-functionalized surface utilizing a microfluidic device has experimentally been investigated under various conditions. The microfluidic devices, fabricated in silicon using DRIE process, consisted of a 5×5 micochamber array; each 1mm×1mm in area, and 50μm in depth. The oxide surface of the microchammber array was functionalized with various antibodies immobilized on a protein G layer. The microfluidic device design allows accurate counting of cells loaded into each microchamber and, thus, enabling a reliable counting of
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Wirth, Rachel, Sangeetha Rajagopalan, Erin Willert, William Null, Jennifer Erdman, Jen-Sing Liu, and Jack P. Higgins. "Abstract 5477: Engineered toxin body demonstrating CD20-specific binding and cell kill in B-cell non-Hodgkin's lymphoma cells." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-5477.

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Bowry, S. K., and M. Tepel. "NON-REVERSIBLE BINDING OF 5'-p-FLUOROSULFONYLBENZOYL ADENOSINE TO WASHED INTACT PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643506.

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ADP is an important in vivo mediator of platelet aggregation. The identification and estimation of a finite number of receptor sites has been made difficult by the reversible nature of the action of ADP on platelets and because ADP can be degraded by enzymes on the platelet surface. Analogues of ADP have therefore been commonly used to study the ADP receptor. We investigated the validity of using 5'-p-fluorosulfonylbenzoyl adenosine (FSBA), an affinity analogue of ADP that inhibits ADP-induced aggregation, for studying the ADP receptor on intact washed platelets. Binding of labelled FSBA, like
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Bowry, S. X. "ENHANCED ASSOCIATION OF FIBRINOGEN WITH ITS PLATELET RECEPTOR DUE TO SODIUM CITRATE INDICATION FOR ONE FIBRINOGEN RECEPTOR ONLY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643701.

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Washed platelet suspensions are almost always prepared from blood anticoagulated with sodium citrate. As citrate has been implicated to affect platelet function and as estimates of the number of fibrinogen (Fbg) bindingsites on platelets range from 4,700 -82,500, we examined the involvement of citrate on the platelet-fibrinogen interaction. The binding of 125-1-fibrinogen to washed platelets from citrated blood (PCB) and thosefrom gel-filtered non-anticoagulated blood (PNB) from the same donor showed linear Scatchard plots for PNB and curvilinear plots for PCB. Assuming the presence of two cla
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Lian, E. C. Y., and F. A. Siddigui. "BINDING OF 37-DKa PLATELET AGGLUTINATING PROTEIN TO HUMAN PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643976.

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We have previously reported the purification of a 37-KDa platelet agglutinating protein (PAP p37) from the plasma of a patient with thrombotic thrombocytopenic purpura. using 125I-labeled p37, the properties of its binding to platelets were studied. The binding of p37 to washed human platelets from 4 normal subjects and two TTP patients after recovery was specific, concentration dependent and saturable. The Scatchard analysis revealed that the binding sites for p37 was about 100,000 per platelet with a dissociation constant of 48 × 10−9 M. The binding of p37 to erythrocytes was very little and
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Cho, Hongkwan, Abdul Sheikh, and Daria A. Narmoneva. "Non-Specific Endothelial Cell Interactions With the Substrate Result in Cell Activation and Angiogenesis In Vitro." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19094.

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Vascularization is critical for success of tissue engineering applications. Previous studies by us and others have shown that self-assembling peptide nanoscaffold RAD16-II promotes de novo capillary formation (angiogenesis) in vitro and neovascularization in vivo, and is a promising material for tissue engineering applications [1, 2]. However, the molecular mechanisms for cell interactions with this material are not known. Angiogenesis is mediated via interactions between integrins, which are expressed on the surface of activated endothelial cells (ECs), and extracellular matrix proteins. Amon
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Reports on the topic "Non-specific binding"

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Gurevitz, Michael, Michael E. Adams, Boaz Shaanan, Oren Froy, Dalia Gordon, Daewoo Lee, and Yong Zhao. Interacting Domains of Anti-Insect Scorpion Toxins and their Sodium Channel Binding Sites: Structure, Cooperative Interactions with Agrochemicals, and Application. United States Department of Agriculture, December 2001. http://dx.doi.org/10.32747/2001.7585190.bard.

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Integrated pest management in modern crop protection may combine chemical and biological insecticides, particularly due to the risks to the environment and livestock arising from the massive use of non-selective chemicals. Thus, there is a need for safer alternatives, which target insects more specifically. Scorpions produce anti-insect selective polypeptide toxins that are biodegradable and non-toxic to warm-blooded animals. Therefore, integration of these substances into insect pest control strategies is of major importance. Moreover, clarification of the molecular basis of this selectivity
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Rafaeli, Ada, and Russell Jurenka. Molecular Characterization of PBAN G-protein Coupled Receptors in Moth Pest Species: Design of Antagonists. United States Department of Agriculture, December 2012. http://dx.doi.org/10.32747/2012.7593390.bard.

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The proposed research was directed at determining the activation/binding domains and gene regulation of the PBAN-R’s thereby providing information for the design and screening of potential PBAN-R-blockers and to indicate possible ways of preventing the process from proceeding to its completion. Our specific aims included: (1) The identification of the PBAN-R binding domain by a combination of: (a) in silico modeling studies for identifying specific amino-acid side chains that are likely to be involved in binding PBAN with the receptor and; (b) bioassays to verify the modeling studies using mut
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Altstein, Miriam, and Ronald Nachman. Rationally designed insect neuropeptide agonists and antagonists: application for the characterization of the pyrokinin/Pban mechanisms of action in insects. United States Department of Agriculture, October 2006. http://dx.doi.org/10.32747/2006.7587235.bard.

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The general objective of this BARD project focused on rationally designed insect neuropeptide (NP) agonists and antagonists, their application for the characterization of the mechanisms of action of the pyrokinin/PBAN (PK-PBAN) family and the development of biostable, bioavailable versions that can provide the basis for development of novel, environmentally-friendly pest insect control agents. The specific objectives of the study, as originally proposed, were to: (i) Test stimulatory potencies of rationally designed backbone cyclic (BBC) peptides on pheromonotropic, melanotropic, myotropic and
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Kelly, Luke. Characteristics of Global Health Diplomacy. Institute of Development Studies (IDS), June 2021. http://dx.doi.org/10.19088/k4d.2021.09.

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This rapid review focuses on Global Health Diplomacy and defines it as a method of interaction between the different stakeholders of the public health sector in a bid to promote representation, cooperation, promotion of the right to health and improvement of health systems for vulnerable populations on a global scale. It is the link between health and international relations. GHD has various actors including states, intergovernmental organizations, private companies, public-private partnerships and non-governmental organizations. Foreign policies can be integrated into national health in vario
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Gurevitz, Michael, William A. Catterall, and Dalia Gordon. face of interaction of anti-insect selective toxins with receptor site-3 on voltage-gated sodium channels as a platform for design of novel selective insecticides. United States Department of Agriculture, December 2013. http://dx.doi.org/10.32747/2013.7699857.bard.

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Voltage-gated sodium channels (Navs) play a pivotal role in excitability and are a prime target of insecticides like pyrethroids. Yet, these insecticides are non-specific due to conservation of Navs in animals, raising risks to the environment and humans. Moreover, insecticide overuse leads to resistance buildup among insect pests, which increases misuse and risks. This sad reality demands novel, more selective, insect killers whose alternative use would avoid or reduce this pressure. As highly selective insect toxins exist in venomous animals, why not exploit this gift of nature and harness t
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Diop, Ahmed. Country Diagnostic Study – Senegal. Islamic Development Bank Institute, October 2021. http://dx.doi.org/10.55780/rp21003.

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The Country Diagnostic Study (CDS) for Senegal uses the Hausmann-Rodrik-Velasco growth diagnostics model to identify the binding constraints being faced in its quest for higher and more sustained economic growth and make recommendations to relax these constraints. Hence, the findings of the CDS can help the Islamic Development Bank in identifying areas where it can have a greater impact and provide an evidence-basis to support the development of the Member Country Partnership Strategy. After decades of subdued and highly volatile economic growth due to heavy dependence on primary commodities a
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Grumet, Rebecca, and Benjamin Raccah. Identification of Potyviral Domains Controlling Systemic Infection, Host Range and Aphid Transmission. United States Department of Agriculture, July 2000. http://dx.doi.org/10.32747/2000.7695842.bard.

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Potyviruses form one of the largest and most economically important groups of plant viruses. Individual potyviruses and their isolates vary in symptom expression, host range, and ability to overcome host resistance genes. Understanding factors influencing these biological characteristics is of agricultural importance for epidemiology and deployment of resistance strategies. Cucurbit crops are subject to severe losses by several potyviruses including the highly aggressive and variable zucchini yellow mosaic virus (ZYMV). In this project we sought to investigate protein domains in ZYMV that infl
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Barg, Rivka, Erich Grotewold, and Yechiam Salts. Regulation of Tomato Fruit Development by Interacting MYB Proteins. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7592647.bard.

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Background to the topic: Early tomato fruit development is executed via extensive cell divisions followed by cell expansion concomitantly with endoreduplication. The signals involved in activating the different modes of growth during fruit development are still inadequately understood. Addressing this developmental process, we identified SlFSM1 as a gene expressed specifically during the cell-division dependent stages of fruit development. SlFSM1 is the founder of a class of small plant specific proteins containing a divergent SANT/MYB domain (Barg et al 2005). Before initiating this project,
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Morrison, Mark, Joshuah Miron, Edward A. Bayer, and Raphael Lamed. Molecular Analysis of Cellulosome Organization in Ruminococcus Albus and Fibrobacter Intestinalis for Optimization of Fiber Digestibility in Ruminants. United States Department of Agriculture, March 2004. http://dx.doi.org/10.32747/2004.7586475.bard.

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Improving plant cell wall (fiber) degradation remains one of the highest priority research goals for all ruminant enterprises dependent on forages, hay, silage, or other fibrous byproducts as energy sources, because it governs the provision of energy-yielding nutrients to the host animal. Although the predominant species of microbes responsible for ruminal fiber degradation are culturable, the enzymology and genetics underpinning the process are poorly defined. In that context, there were two broad objectives for this proposal. The first objective was to identify the key cellulosomal component
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Landau, Sergei Yan, John W. Walker, Avi Perevolotsky, Eugene D. Ungar, Butch Taylor, and Daniel Waldron. Goats for maximal efficacy of brush control. United States Department of Agriculture, March 2008. http://dx.doi.org/10.32747/2008.7587731.bard.

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Background. Brush encroachment constitutes a serious problem in both Texas and Israel. We addressed the issue of efficacy of livestock herbivory - in the form of goat browsing - to change the ecological balance to the detriment of the shrub vegetation. Shrub consumption by goats is kept low by plant chemical defenses such as tannins and terpenes. Scientists at TAES and ARO have developed an innovative, cost-effective methodology using fecal Near Infrared Spectrometry to elucidate the dietary percentage of targeted, browse species (terpene-richredberry and blueberry juniper in the US, and tanni
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