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Journal articles on the topic 'NOS terminator'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Kushnir, G. V. "DISTRIBUTION OF TRANSGENIC PLANTS IN UKRAINE – CURRENT STATE." Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology 22, no. 2 (2021): 225–29. http://dx.doi.org/10.36359/scivp.2021-22-2.25.

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Scientific achievement in molecular biology and genetic engineering have made it possible to create new methods of selection k that are based on genome modification. Todays, more and more genetically modified plants are being intensively tested in the field, but their influence has not yet been fully studied on the environment, biodiversity, human health, and the animal body. The article analyzes the results of research on the presence of GM ingredients in plant raw materials, processed products, feed for productive and unproductive animals, etc. for the period 2019-2020. Studies on the

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Dan, Junhao, Huafeng Deng, Yumei Xia, et al. "Application of the FLP/LoxP-FRT recombination system to switch the eGFP expression in a model prokaryote." Open Life Sciences 17, no. 1 (2022): 172–79. http://dx.doi.org/10.1515/biol-2022-0019.

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Abstract In prokaryotes, few studies have applied the flippase (FLP)/P1-flippase recombination target (LoxP-FRT) recombination system to switch gene expression. This study developed a new method for switching gene expression by constructing an FLP/LoxP-FRT site-specific recombination system in Escherichia coli. To this end, we placed the Nos terminator flanked by a pair of LoxP-FRT in front of enhanced green fluorescent protein (eGFP). The Nos terminator was used to block the expression of the eGFP. When a plasmid expressing FLP was available, deletion of the Nos terminator would allow express

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3

Rashid, Rizwan. "Genetic Transformation of Tomato (Solanum Lycopersicum. L) Using Cry1Ac Gene to Impart Resistance Against Fruit Borer." Journal of Natural Science Review 2, Special.Issue (2024): 332–39. http://dx.doi.org/10.62810/jnsr.v2ispecial.issue.136.

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Particle gun-mediated genetic transformation was used to produce transgenic plants of tomato (Solanumlycopersicum L.) resistant to fruit borer. Cotyledon and leaf explants from invitro tomato seedlings of variety ‘Punjab Upma’ with Cry1Ac gene construct (pGEM-4Z) were driven by Ubi promoter and NOS terminator, and GUS gene construct (pWRG 1515) were driven by CaMV 35S promoter and NOS terminator. The constructs were maintained as plasmids in E. coli strain JM109. These results signify the successful introduction of the Cry1Ac gene into tomato plants. Further PCR analysis for the Cry1Ac gene of

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Osyanin, Konstantin A., Dinis A. Mirgazov, Inna A. Elizarova, Ekaterina A. Dodonova, and Nail A. Fakhrutdinov. "Identification of regulatory sequences of the 35S promoter and NOS terminator in agricultural products." Butlerov Communications 64, no. 12 (2020): 1–10. http://dx.doi.org/10.37952/roi-jbc-01/20-64-12-1.

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The pace of scientific and technological progress is steadily growing every day, in this regard much attention is paid to the problems of using genetically modified organisms (GMOs) in agricultural products, since there are risks of negative impact on human health and the environment. Thus, there is a need for constant monitoring of all agricultural products for the content of GMO regulatory sequences. This work is devoted to studying the frequency of occurrence of GMO fragments in the products of agricultural enterprises of the Republic of Tatarstan. The material describes the monitoring of a

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5

Hardegger, M., P. Brodmann, and A. Herrmann. "Quantitative detection of the 35S promoter and the NOS terminator using quantitative competitive PCR." European Food Research and Technology 209, no. 2 (1999): 83–87. http://dx.doi.org/10.1007/s002170050462.

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6

Oraby, Hanaa AS, Amal A. Hassan, and Ahlam A. Abou Mossallam. "Screening food products for the presence of CaMV 35S promoter and NOS 3′ terminator." Journal of the Science of Food and Agriculture 85, no. 12 (2005): 1974–80. http://dx.doi.org/10.1002/jsfa.2201.

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7

Mano, Junichi, Shuko Hatano, Yasuaki Nagatomi, Satoshi Futo, Reona Takabatake, and Kazumi Kitta. "Highly Sensitive GMO Detection Using Real-Time PCR with a Large Amount of DNA Template: Single-Laboratory Validation." Journal of AOAC INTERNATIONAL 101, no. 2 (2018): 507–14. http://dx.doi.org/10.5740/jaoacint.17-0197.

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Abstract Current genetically modified organism (GMO) detection methods allow for sensitive detection. However, a further increase in sensitivity will enable more efficient testing for large grain samples and reliable testing for processed foods. In this study, we investigated real-time PCR-based GMO detection methods using a large amount of DNA template. We selected target sequences that are commonly introduced into many kinds of GM crops, i.e., 35S promoter and nopaline synthase (NOS) terminator. This makes the newly developed method applicable to a wide range of GMOs, including some unauthor

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8

Lipp, Markus, Peter Brodmann, Klaus Pietsch, et al. "IUPAC Collaborative Trial Study of a Method To Detect Genetically Modified Soy Beans and Maize in Dried Powder." Journal of AOAC INTERNATIONAL 82, no. 4 (1999): 923–28. http://dx.doi.org/10.1093/jaoac/82.4.923.

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Abstract This paper presents results of a collaborative trial study (IUPAC project No. 650/93/97) involving 29 laboratories in 13 countries applying a method for detecting genetically modified organisms (GMOs) in food. The method is based on using the polymerase chain reaction to determine the 35S promoter and the NOS terminator for detection of GMOs. Reference materials were produced that were derived from genetically modified soy beans and maize. Correct identification of samples containing 2% GMOs is achievable for both soy beans and maize. For samples containing 0.5% genetically modified s

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Safaei, Payam, Ebrahim Molaee Aghaee, Gholamreza Jahed Khaniki, Setareh Agha Kuchak Afshari, and Sassan Rezaie. "A simple and accurate PCR method for detection of genetically modified rice." Journal of Environmental Health Science and Engineering 17, no. 2 (2019): 847–51. http://dx.doi.org/10.1007/s40201-019-00401-x.

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Abstract Background Legislation regulating for labeling and use of genetically modified (GM) crops are increased considerably worldwide in order to health and safety assurance of consumers. For this purpose, a polymerase chain reaction (PCR) method has been developed for detection of GM rice in people’s food diet. Methods In this study, eighty-one non-labeled rice samples were collected randomly from different market sites of Tehran, Iran. In order to analysis, rice genomic DNA was extracted using MBST DNA extraction kit and subsequently, sucrose phosphate synthase (SPS) gene was used to confi

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10

Kushnir, G. V. "Monitoring of plant raw materials and feed for animal in 2020 for the presence of GM-ingredients." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 23, no. 103 (2021): 141–44. http://dx.doi.org/10.32718/nvlvet10319.

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The creation and the use of genetically modified products has become a tendency in the development of agricultural and food technologies. The area of agricultural land under genetically modified plants is constantly growing. Todays the process of using GMOs and the expediency of their creation is a debatable issue. The modification of the genome of traditional agricultural cultures gives them resistance to pesticides, pests, diseases, which cause to the significant an increase of harvest and improved quality and taste characteristics. However, the effects of GMOs on the environment and the bod

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11

Khabibrakhmanov, I. K., D. A. Degenstein, and E. J. Llewellyn. "Mesospheric ozone: Determination from orbit with the OSIRIS instrument on Odin." Canadian Journal of Physics 80, no. 4 (2002): 493–504. http://dx.doi.org/10.1139/p02-022.

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The analysis of the data from the optical spectrograph and infrared imager system (OSIRIS) that will fly on the Odin satellite requires special attention as many of the measurements will be made in regions of the atmosphere that are relatively close to terminator. Under these conditions the photochemical processes in the upper atmosphere that are responsible for much of the oxygen infrared atmospheric band airglow emission are nonstationary. It is this latter aspect that complicates the retrieval of the mesospheric ozone profile from the OSIRIS observations. However, a tomographic analysis tec

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12

He, Yanhua, and Zhefeng Fan. "A novel biosensor based on DNA hybridization for ultrasensitive detection of NOS terminator gene sequences." Sensors and Actuators B: Chemical 257 (March 2018): 538–44. http://dx.doi.org/10.1016/j.snb.2017.10.183.

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13

Aarif, Ghaith Lotfi, Bilal Kamil Sulaiman, and Zahra M. Alkhafaji. "Screening of genetically modified Corn Zea mays in Iraqi market." Journal of Biotechnology Research Center 7, no. 1 (2013): 21–29. http://dx.doi.org/10.24126/jobrc.2013.7.1.236.

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Detection of the genetically modified crops could be done by screening certain markers usually used in modification. In this study polymerase chain reaction (PCR) technology was used to investigate the presence of the promoter P35s and nos terminator in the genetically modified corn Zea mays. 72 samples of the maize crop collected from inside Iraqi market from various sources, including imported crops and other local strains used for agriculture or for the production of animal feed. DNA extracted from the corn seeds by two methods, the efficiency of extraction was compared between the two proc

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14

Hồng, La Việt, Bùi Phương Thảo, Phạm Bích Ngọc та Chu Hoàng Hà. "NGHIÊN CỨU TĂNG CƯỜNG BIỂU HIỆN CỦA MIRACULIN TRONG CÂY CÀ CHUA VIỆT NAM". TNU Journal of Science and Technology 226, № 14 (2021): 276–83. http://dx.doi.org/10.34238/tnu-jst.4923.

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Miraculin là một protein tự nhiên có khả năng chuyển vị chua thành vị ngọt mặc dù đặc tính của nó không ngọt. Trong nghiên cứu này, mã di truyền của miraculin được tối ưu. Ngoài ra, nhằm tăng cường sự biểu hiện của miraculin trong cây cà chua chuyển gen, promoter E8 đặc hiệu ở quả từ cây cà chua và terminator HSP từ cây A.thaliana cũng được sử dụng để điều khiển hoạt động của gen miraculin tổng hợp. Kết quả phân tích bằng PCR cho thấy có 16 dòng cà chua mang cấu trúc E8-pro:Mir:HSP-ter và 11 dòng cà chua được chuyển cấu trúc 35S-pro:Mir:NOS-ter, hiệu suất chuyển gen tương ứng là 42,11% và 52,3

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15

Sun, Wei, Jianghua Zhong, Bo Zhang, and Kui Jiao. "Application of cadmium sulfide nanoparticles as oligonucleotide labels for the electrochemical detection of NOS terminator gene sequences." Analytical and Bioanalytical Chemistry 389, no. 7-8 (2007): 2179–84. http://dx.doi.org/10.1007/s00216-007-1661-9.

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16

Meric, Burcu, Kagan Kerman, Giovanna Marrazza, Ilaria Palchetti, Marco Mascini, and Mehmet Ozsoz. "Disposable genosensor, a new tool for the detection of NOS-terminator, a genetic element present in GMOs." Food Control 15, no. 8 (2004): 621–26. http://dx.doi.org/10.1016/j.foodcont.2003.10.004.

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17

Kianfar, Niloofar, and Maryam Heydarifard. "Rapid and accurate detection of genetically manipulated soybean using polymerase chain reaction." MOJ Current Research & Reviews 2, no. 2 (2019): 37–40. http://dx.doi.org/10.15406/mojcrr.2019.02.00053.

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Background: It is controversy over the GM products consumption around the world. However, the limitations of food supplies and population growth have forced the posterity to use transgenic products. Accurate strategies for consumption and use of proper diagnostic methods are crucial in this regard. Materials and methods: The soybean commercial samples were purchased as a control from supermarkets located in Tehran. Considerable attention was paid to collecting samples from different brands. In this investigation, in order to identify the soybean oil in products, randomly, 25 raw oil and soybea

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18

Singh, Monika, Deepa Pal, Payal Sood, and Gurinderjit Randhawa. "Construct-Specific Loop-Mediated Isothermal Amplification: Rapid Detection of Genetically Modified Crops with Insect Resistance or Herbicide Tolerance." Journal of AOAC INTERNATIONAL 103, no. 5 (2020): 1191–200. http://dx.doi.org/10.1093/jaoacint/qsaa043.

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Abstract Background Insect resistant and herbicide tolerant genetically modified (GM) events have been approved in many countries. Screening methods could facilitate preliminary testing to check the GM status, which may target control elements, transgenes, and marker genes or construct regions. Among these, methods targeting the construct region, i.e., the junction between two genetic elements of a transgenic cassette are more specific. Objective Loop-mediated isothermal amplification (LAMP) assays targeting three construct regions were developed; between Cauliflower Mosaic Virus 35S promoter

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19

Xu, Chao, Liang Li, Wujun Jin, and Yusong Wan. "Recombinase Polymerase Amplification (RPA) of CaMV-35S Promoter and nos Terminator for Rapid Detection of Genetically Modified Crops." International Journal of Molecular Sciences 15, no. 10 (2014): 18197–205. http://dx.doi.org/10.3390/ijms151018197.

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20

Wang, Yuling, Cheng Peng, Lin Ding, et al. "An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a." Foods 12, no. 3 (2023): 615. http://dx.doi.org/10.3390/foods12030615.

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CRISPR/Cas12a technology is used for nucleic acid detection due to its specific recognition function and non-specific single-stranded DNA cleavage activity. Here, we developed a fluorescence visualisation detection method based on PCR and CRISPR/Cas12a approaches. The method was used to detect the nopaline synthase terminator (T-nos) of genetically modified (GM) crops, circumventing the need for expensive instruments and technicians. For enhanced sensitivity and stability of PCR-CRISPR/Cas12a detection, we separately optimised the reaction systems for PCR amplification and CRISPR/Cas12a detect

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21

Su, Ailing, Ziqi Chen, Weiqing Xu, Chongyang Liang, Xiangguo Liu, and Shuping Xu. "CRISPR/dCas9-assisted SERS sensing for transgenic crops based on two-factor verification of 35S promoter and Nos terminator." Sensors and Actuators B: Chemical 429 (April 2025): 137293. https://doi.org/10.1016/j.snb.2025.137293.

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22

Xu, Chenshan, Xiaoli Zhu, Aihong Xu, Jian Song, and Shuxia Liang. "Construction and validation of co-expression vector for rice alpha tubulin and microtubule associated protein respectively fused with fluorescent proteins." PeerJ 12 (September 26, 2024): e18118. http://dx.doi.org/10.7717/peerj.18118.

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Microtubule (MT) consists of α-tubulin and β-tubulin. The dynamic instability regulated by various microtubule associated proteins (MAPs) is essential for MT functions. To analyze the interaction between tubulin/MT and MAP in vivo, we usually need tubulin and MAP co-expressed. Here, we constructed a dual-transgene vector expressing rice (Oryza sativa) α-tubulin and MAP simultaneously. To construct this vector, plant expression vector pCambia1301 was used as the plasmid backbone and Gibson assembly cloning technology was used. We first fused and cloned the GFP fragment, α-tubulin open reading f

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23

Singh, Chandra K., Abhishek Ojha, and Devendra N. Kachru. "Detection and Characterization of cry1Ac Transgene Construct in Bt Cotton: Multiple Polymerase Chain Reaction Approach." Journal of AOAC INTERNATIONAL 90, no. 6 (2007): 1517–25. http://dx.doi.org/10.1093/jaoac/90.6.1517.

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Abstract To comply with international labeling regulations for genetically modified (GM) crops and food, and to enable proper identification of GM organisms (GMOs), effective methodologies and reliable approaches are needed. The spurious and unapproved GM planting has contributed to crop failures and commercial losses. To ensure effective and genuine GM cultivation, a methodology is needed to detect and identify the trait of interest and concurrently evaluate the structural and functional stability of the transgene insert. A multiple polymerase chain reaction (PCR) approach was developed for d

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Spanea, Eleni, Theofania Tsironi, Efstathia Tsakali, et al. "Evaluation of a Real Time PCR Assay Method for the Detection of Genetically Modified Organisms in Food Products." Journal of Food Research 9, no. 2 (2020): 1. http://dx.doi.org/10.5539/jfr.v9n2p1.

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The objective of the study was to determine qualitatively by validated Real Time PCR method the occurrence of genetically modified maize and soybean in commercial food products from the Greek market. 70 independent samples were collected, including products from different categories (i.e. cereal based, biscuits and snacks) which declared either corn or soybean on the labelling. The result of the study indicated that 37.1% of maize and soy products (n=70) displayed in the Greek market have detectable levels of genetically modified maize or soy. These products were identified by specific primers

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Wardani, Agustin Krisna, Annisa Alirsyah, and Ana Fauziah. "Identifikasi Gen Transgenik pada Produk Susu Bubuk Kedelai dan Susu Formula Soya dengan Metode PCR (Polymerase Chain Reaction)." Agritech 37, no. 3 (2018): 237. http://dx.doi.org/10.22146/agritech.16656.

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The need of soybean that reached up to 2,2 million tons per year has made Indonesia imports this commodity 1,62 million tons from countries that adopt Genetically Modified (GM) soybean. By the presence of GM soybeans in Indonesia, Genetically Modified Product (GMP) labelling has need to be done. Detection of GMP can be done by using PCR. The aim of this study was to determine the presence of trangenic genetic material in soy milk powder and soy formula products to classify as GMP. Another goal was to determine the optimum annealing temperature of the primers used. Based on this study, the opti

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Barbau-Piednoir, Elodie, Antoon Lievens, Guillaume Mbongolo-Mbella, et al. "SYBR®Green qPCR screening methods for the presence of “35S promoter” and “NOS terminator” elements in food and feed products." European Food Research and Technology 230, no. 3 (2009): 383–93. http://dx.doi.org/10.1007/s00217-009-1170-5.

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Pospieszny, H., M. Cajza, and R. Plewa. "First Report of Zucchini yellow mosaic virus in Cucumber in Poland." Plant Disease 87, no. 11 (2003): 1399. http://dx.doi.org/10.1094/pdis.2003.87.11.1399a.

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In June 2002, mosaic and interveinal chlorosis were observed on two cucumber plants (Cucumis sativus) grown in one commercial greenhouse in the western region of Poland. Electron microscopic examination of leaf-dip preparations from infected plants showed flexuous filamentous virus particles typical of potyviruses (720 to 750 nm long). Chenopodium amaranticolor, Chenopodium quinoa, Citrullus lanatus, C. melo, C. sativus, Cucurbita maxima, Cucurbita pepo, Cucurbita pepo cv. Giromontiina, Cucurbita pepo cv. Patissoniana, Nicotiana benthamiana, and N. tabacum were mechanically inoculated with sap

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Ovesná, J., L. Dedičová, J. Horáček, E. Sadilová, L. Kučera, and L. Měsková. "Comparison of different PCR-based protocols for detection of Roundup Ready soybean." Czech Journal of Genetics and Plant Breeding 38, No. 1 (2012): 55–63. http://dx.doi.org/10.17221/6111-cjgpb.

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Genetically modified organisms have become a part of our environment and food chain. Roundup Ready soybean is at the moment the most frequent one that man can meet. National regulations require careful monitoring and detection of GMOs. We present in this investigation comparison of several protocols and individual steps, which are included in the whole detection procedure. Currently used CTAB based protocol is suitable for DNA isolation from the green plant tissue but also from the flour. Lectin coding sequence specific primers were suitable for soybean DNA detection unlike leu-tRNA gene speci

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Saienko, Artem, Mykyta Peka, Viktor Balatsky, Sergii Korinnyi, and Oleksandr Tsereniuk. "GMO analysis technique based on PCR and real-time PCR methods." Pig breeding the interdepartmental subject scientific digest, no. 75-76 (December 7, 2021): 58–67. http://dx.doi.org/10.37143/0371-4365-2021-75-76-06.

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The use of genetically modified organisms (GMOs) is promising for overcoming the shortage of food products in the world and solving the problem of hunger arising in various regions. At the same time, the use of GMOs has become a cause of debate, as the safety of consuming GMO products for human health remains unproven. The risks associated with GMOs cause public concern, which has led to the restriction of the use of GMOs and their products in many countries and the need for constant control of their content in food products. This study describes methods based on the polymerase chain reaction

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Bakhsh, Allah, Muhammad Aasim, Muhammad Abu Bakar Zia, et al. "First Report of Agrobacterium tumefaciens mediated genetic transformation of aquatic Rice paddy herb (Limnophila aromatica)." Turkish Journal of Agriculture - Food Science and Technology 4, no. 8 (2016): 642. http://dx.doi.org/10.24925/turjaf.v4i8.642-645.747.

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The study presents first report of Agrobacterium mediated genetic transformation in Rice paddy herb (Linmophila aromatica). A. tumefaciens strain C58C1 harboring pBin19 Plasmid containing β-glucuronidase (GUS) and neomycin phosphotransferase II (nptII) gene, under the control of 35S promoter and NOS terminator was used. Shoot tip explants were inoculated for 30 min followed by co-cultivation for 72 h and selected on agar semi solidified MS medium containing 100 mg/l Kanamycin and 1.0 mg/l BA; whereas total number of 78 putative transgenic shoots were obtained. The shoots were rooted on MS medi

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Gardner, Courtney M., Carley A. Gwin, and Claudia K. Gunsch. "A survey of crop-derived transgenes in activated and digester sludges in wastewater treatment plants in the United States." Water Science and Technology 77, no. 7 (2018): 1810–18. http://dx.doi.org/10.2166/wst.2018.051.

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Abstract The use of transgenic crops has become increasingly common in the United States over the last several decades. Increasing evidence suggests that DNA may be protected from enzymatic digestion and acid hydrolysis in the digestive tract, suggesting that crop-derived transgenes may enter into wastewater treatment plants (WWTPs) intact. Given the historical use of antibiotic resistance genes as selection markers in transgenic crop development, it is important to consider the fate of these transgenes. Herein we detected and quantified crop-derived transgenes in WWTPs. All viable US WWTP sam

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Liang, Qianqian, Xiaohui Wang, Qingqing Xie, et al. "Development of highly sensitive one-pot ERA-CRISPR assays for on-site detection of CaMV35S promoter and NOS terminator in genetically modified crops." Food Control 170 (April 2025): 111049. http://dx.doi.org/10.1016/j.foodcont.2024.111049.

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Ovesná, J., L. Kučera, J. Hodek, and K. Demnerová. "Reliability of PCR based screening for identification and quantification of GMOs." Czech Journal of Food Sciences 28, No. 2 (2010): 133–38. http://dx.doi.org/10.17221/67/2009-cjfs.

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Handling with genetically modified organisms (GMOs) is regulated namely in EC. Laboratories often use polymerase chain reaction (PCR) based screening methods to monitor the presence of GM particles in food commodities as a cost effective approach. The reliability was tested of such screening using 35S CaMV promoter as the target sequences. Soya grown from non-GM cultivar as declared by a seed company was investigated after the harvest, transport to the silo, and before processing. The results based on PCR and real-time PCR analysis clearly showed that, the contamination with debris of other sp

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Korwin-Kossakowska, A., K. Sartowska, A. Linkiewicz, G. Tomczyk, B. Prusak, and G. Sender. "Evaluation of the effect of genetically modified Roundup Ready soya bean and MON 810 maize in the diet of Japanese quail on chosen aspects of their productivity and retention of transgenic DNA in tissues." Archives Animal Breeding 56, no. 1 (2013): 597–606. http://dx.doi.org/10.7482/0003-9438-56-060.

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Abstract. The objective of the study was to evaluate the impact of genetically modified (GM) ingredients (soya bean meal and maize grain) used in poultry diets on birds' performance, as well as the accumulation of the transgenic DNA in eggs, breast muscle and internal organs. In the present experiment four generations of Japanese quails, which were subject to three different diets, two containing genetically modified organisms (GMO, soya and maize) and one GMO-free control, were analysed. Birds' performance traits were monitored along the trial. A screening molecular method – polymerase chain

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Faith, F. Kavishe, Chimwamurombe Percy, and Kennedy Jeya. "A Survey of Genetically Modified Foods and Feed in Namibian Supermarkets." Cognizance Journal of Multidisciplinary Studies 2, no. 8 (2022): 85–92. https://doi.org/10.47760/cognizance.2022.v02i08.003.

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<em>The current study was carried out to survey if genetically modified organisms (GMOs) are present in feeds and foods found in local supermarkets in Namibia. Little is known of the presence and prevalence of GMOs in the local supermarkets or information available on the labelling provided on some. The aim was to prove the absence or existence of GMOs in the foods and feeds by means of molecular extraction and screening procedures. Samples purposefully selected were nine (6 food and 3 chicken feed samples). DNA was extracted from them, and they were screened by means of polymerase chain react

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Hassan, L. "Successful genetic transformation in date palm (Phoenix dactylifera)." Journal of the Bangladesh Agricultural University 11, no. 2 (2014): 171–76. http://dx.doi.org/10.3329/jbau.v11i2.19841.

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The introduction of foreign genes into most of the Phoenix spp using recombinant DNA technology is not a straight forward task. In Phoenix spp application of this technology towards successful transformation proved to be a more difficult one so far no report on the successful regeneration of transgenic date palm plants has been published. We developed an efficient and reproducible variety-independent method for producing transgenic date palm (Phoenix spp) via Agrobacterium-mediated transformation. Agrobacterium rhizogenes strains LBA 9402 were used and for cotransformation experiments the st

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Liu, Hai-nan, Jian-rong Feng, Xiao-fang Liu, Wen-hui Li, Wen-juan Lv, and Ming Luo. "Cloning of the Self-incompatibility SFB Gene from Chinese Apricot ‘Xiaobaixing’ and Construction of the SFB Expression Vectors." Journal of the American Society for Horticultural Science 141, no. 5 (2016): 407–13. http://dx.doi.org/10.21273/jashs03722-16.

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Three kinds of expression vectors of a pollen-S determinant were constructed to provide a reference for molecular breeding of self-compatible (SC) Prunus species. An S-haplotype-specific F-box (SFB) protein gene from the ‘Xiaobaixing’ apricot (Prunus armeniaca) was cloned by reverse transcription polymerase chain reaction (RT-PCR) and 3′-rapid-amplification of cDNA ends (3′-RACE). A 1136-bp sequence complementary to the 3′-end of the cDNA (GenBank accession number KP938528.2) with a 912-bp complete open reading frame (ORF) was obtained. The deduced amino acid sequence contained an F-box domain

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Curticăpean, Manuela-Claudia. "ANALYSIS OF DIFFERENT TYPES OF HONEY TO EMPHASIZE GENETICALLY MODIFIED SEQUENCES." Acta Biologica Marisiensis 7, no. 1-2 (2024): 46–52. https://doi.org/10.62838/abmj-2024-0005.

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Food safety and consumer health protection is a particularly important aspect, always in the attention of researchers. The authentication of honey, but also its mislabelling, is one of the current challenges related to quality monitoring. Therefore, a rigorous monitoring of the different types of honey on the market is required, to ascertain whether they are properly labeled and to detect the possible presence of modified genes. The aim of this study was to analyze honey samples, to identify the possible presence of genetically modified genes, using the technique based on DNA analysis, called

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Yu, Yanbo, Rui Li, Zonghua Ma, et al. "Development and evaluation of a novel loop mediated isothermal amplification coupled with TaqMan probe assay for detection of genetically modified organism with NOS terminator." Food Chemistry 356 (September 2021): 129684. http://dx.doi.org/10.1016/j.foodchem.2021.129684.

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Park, Saet-Byul, Ji-Yeong Kim, Do-Geun Lee, Jae-Hwan Kim, Min-Ki Shin, and Hae-Yeong Kim. "Development of a Systematic qPCR Array for Screening GM Soybeans." Foods 10, no. 3 (2021): 610. http://dx.doi.org/10.3390/foods10030610.

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A screening method using the 35S promoter and nos terminator for genetically modified organisms (GMOs) is not sufficient to cover all GM soybean events. In this study, a real-time polymerase chain reaction (also known as quantitative polymerase chain reaction, qPCR) array targeting eight screening assays combined with a prediction system was developed for the rapid tracking of GM soybeans. Each assay’s specificity was tested and confirmed using 17 GM soybean events that have been approved in Korea. The sensitivity of each assay was determined to range from 0.01% to 0.05% using DNA mixtures wit

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Wang, Lishuan, Chunlei Wang, Xinye Liu та ін. "Peroxisomal β-oxidation regulates histone acetylation and DNA methylation in Arabidopsis". Proceedings of the National Academy of Sciences 116, № 21 (2019): 10576–85. http://dx.doi.org/10.1073/pnas.1904143116.

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Epigenetic markers, such as histone acetylation and DNA methylation, determine chromatin organization. In eukaryotic cells, metabolites from organelles or the cytosol affect epigenetic modifications. However, the relationships between metabolites and epigenetic modifications are not well understood in plants. We found that peroxisomal acyl-CoA oxidase 4 (ACX4), an enzyme in the fatty acid β-oxidation pathway, is required for suppressing the silencing of some endogenous loci, as well as Pro35S:NPTII in the ProRD29A:LUC/C24 transgenic line. The acx4 mutation reduces nuclear histone acetylation a

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Mukhammadiyeva, G. F., D. O. Karimov, E. R. Shaikhlislamova, et al. "Identification of genetically modified organisms in foods of plant origin as a way to control health risks for consumers." Health Risk Analysis, no. 3 (September 2022): 83–89. http://dx.doi.org/10.21668/health.risk/2022.3.07.

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Uncontrolled distribution of goods produced by genetically modified plants should be prevented by the state in order to secure food safety in the Russian Federation and to minimize health risks for consumers. We analyzed foods of plant origin for children to identify components of genetically modified organisms in them. It was done to ensure safety of such foods. The highest specific weight among the analyzed foods belonged to nectars (40.0 %) and juice-containing drinks (36.0 %). Juices and fruit drinks accounted for 12 % each. Genetically modified organisms were determined in foods by identi

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Randhawa, Gurinder Jit, Ruchi Sharma, and Monika Singh. "Qualitative and Event-Specific Real-Time PCR Detection Methods for Bt Brinjal Event EE-1." Journal of AOAC INTERNATIONAL 95, no. 6 (2012): 1733–39. http://dx.doi.org/10.5740/jaoacint.11-478.

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Abstract Bt brinjal event EE-1 with cry1Ac gene, expressing insecticidal protein against fruit and shoot borer, is the first genetically modified food crop in the pipeline for commercialization in India. Qualitative polymerase chain reaction (PCR) along with event-specific conventional as well as real-time PCR methods to characterize the event EE-1 is reported. A multiplex (pentaplex) PCR system simultaneously amplifying cry1Ac transgene, Cauliflower Mosaic Virus (CaMV) 35S promoter, nopaline synthase (nos) terminator, aminoglycoside adenyltransferase (aadA) marker gene, and a taxon-specific β

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Waluyo, Seagames, Sustiprijatno Sustiprijatno, and Suharsono Suharsono. "Transformasi Genetik Tembakau dengan Gen Cold Shock Protein melalui Perantara Agrobacterium tumefaciens." Jurnal AgroBiogen 9, no. 2 (2016): 58. http://dx.doi.org/10.21082/jbio.v9n2.2013.p58-65.

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<p>Cold<br />shock protein (Csp) essential for organisms to survive in<br />abiotic stress condition. CspB gene has been fused to<br />ubiquitin promoter in the T-DNA region of pCambia 1300int,<br />and introduced into Agrobacterium tumefaciens LBA4404.<br />This research had an objective to transform genetically<br />Nicotiana tabacum cv. Samsun by CspB gene under the<br />control of Ubiquitin promoter and NOS terminator mediated<br />by A. tumefaciens. Leaf discs were co-cultivated with A.<br />tumefaciens LBA 4404. Based on the num

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Plchová, Helena, Tomáš Moravec, Noemi Čeřovská, et al. "A GoldenBraid-Compatible Virus-Based Vector System for Transient Expression of Heterologous Proteins in Plants." Viruses 14, no. 5 (2022): 1099. http://dx.doi.org/10.3390/v14051099.

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We have developed a Potato virus X (PVX)-based vector system compatible with the GoldenBraid 2.0 (GB) cloning strategy to transiently express heterologous proteins or peptides in plants for biotechnological purposes. This vector system consists of three domestication vectors carrying three GB parts—the cauliflower mosaic virus (CaMV) 35S promoter with PVX upstream of the second subgenomic promoter of the PVX coat protein (PVX CP SGP), nopaline synthase (NOS) terminator with PVX downstream of the first PVX CP SGP and the gene of interest (GOI). The full-length PVX clone carrying the sequence en

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Danial, G. H., D. A. Ibrahim, and G. Q. Song. "AGROBACTERIUM-MEDIATED TRANSFORMATION OF TWO TOMATO CULTIVARS (LYCOPERSICON ESCULENTUM MILL.) CV. SANDRA AND ROCKY." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 52, no. 3 (2021): 745–55. http://dx.doi.org/10.36103/ijas.v52i3.1366.

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An efficient protocol for Agrobacterium-mediated transformation of tomato cultivars Sandra and Rocky was conducted to examine the possibility of producing transgenic tomato plants cultivars harbouring the nptII gene, conferring kanamycin resistance. To achieve this aim, tomato cotyledon explants were transformed using EHA105 Agrobacterium tumefaciens strain harboring the binary vectors pBI121 which contains Gus gene, and neomycin phosphotransferase II (nptII) as selectable marker gene under the control of a CaMV35S promoter and nopaline synthase (nos) Terminator. Transformant detection was car

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Li, Liang, Mei Dong, Na An, Lixia Liang, Yusong Wan, and Wujun Jin. "A Novel Reference Plasmid for the Qualitative Detection of Genetically Modified Rice in Food and Feed." BioMed Research International 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/948297.

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Rice is one of the most important food crops in the world. Genetically modified (GM) technology has been used in rice to confer herbicide tolerance and pathogen or insect resistance. China invests heavily in research on GM rice. By the end of 2014, at least 250 transgenic rice lines had been developed in China. To monitor the presence of GM rice in food and feed, we collected information on foreign elements from 250 transgenic rice lines and found 5 elements, including theAgrobacterium tumefaciensnopaline synthase terminator (T-NOS), the cauliflower mosaic virus 35S promoter (CaMV35S), the ubi

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Liu, Guang-Ming, Hui-Nong Cai, Min-Jie Cao, and Wen-Jin Su. "Fluorophore double stranded probe-multiplex quantitative PCR method for detecting transgenic component of promoter derived from Cauliflower Mosaic Virus and nos terminator derived from Agrobacterium tumefaciens simultaneously." Food Control 18, no. 6 (2007): 615–22. http://dx.doi.org/10.1016/j.foodcont.2005.07.012.

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Kavishe, Faith F., Percy Chimwamurombe, and Jeya Kennedy. "A Survey of Genetically Modified Foods and Feed in Namibian Supermarkets." Cognizance Journal of Multidisciplinary Studies 2, no. 8 (2022): 85–92. http://dx.doi.org/10.47760/cognizance.2022.v02i08.003.

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The current study was carried out to survey if genetically modified organisms (GMOs) are present in feeds and foods found in local supermarkets in Namibia. Little is known of the presence and prevalence of GMOs in the local supermarkets or information available on the labelling provided on some. The aim was to prove the absence or existence of GMOs in the foods and feeds by means of molecular extraction and screening procedures. Samples purposefully selected were nine (6 food and 3 chicken feed samples). DNA was extracted from them, and they were screened by means of polymerase chain reaction

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Bobrownyzky, Juri. "TRANSGENIC CULTIVARS: CREATION, CULTIVATION, AND DIAGNOSTICS." Interdepartmental thematic scientific collection "Potato research", no. 47 (December 30, 2022): 64–73. https://doi.org/10.52087/0130-2094.2022.47.05.

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This mini-review is devoted to the issues of creation, cultivation and diagnostics of cultivars originating from modern technology of genetic modification (GM cultivars). A GM cultivar is defined as follows: it should be created by means of recombinant DNA methods or cell fusion technology. These technologies arose in the 70th years of 20th century, and as soon as in 1990 the first commercial cultivar (tobacco with resistance to viruses) was registered. Nowadays the area under GM crops is estimated to be around 160 million hectares. Sceptical attitude towards GM technology resulted in regulati

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