Relevant bibliographies by topics / Ns3

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
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Academic literature on the topic 'Ns3'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 March 2023

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Journal articles on the topic "Ns3"

1

VERMA, RAKESH KUMAR, YASHBIR SINGH SHIVAY, MUKESH CHOUDHARY, PRAKASH CHAND GHASAL, and RAGHAVENDRA MADAR. "Nutrient mobilization and crop assimilation as influenced by nutrient management strategies under direct seeded basmati rice (Oryza sativa) – based cropping systems." Indian Journal of Agricultural Sciences 90, no. 10 (December 4, 2020): 1894–901. http://dx.doi.org/10.56093/ijas.v90i10.107891.

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The field experiment was carried out for two consecutive years (2014–2016) in split-plot design to investigate the effect of integrated nutrient management and crop diversification through inclusion of legume and vegetable crops in direct seeded basmati rice (Oryza sativa L.)–based cropping systems (DSRB) on nutrient availability for crop uptake. The study involved four cropping systems (CS) in main plots (DSBR‒wheat‒fallow (CS1), DSBR‒wheat‒greengram (CS2), DSBR‒cabbage‒greengram (CS3) and DSBR‒cabbage‒onion (CS4) and four nutrient management strategies under subplots (unfertilized (NS0), 100% recommended dose of fertilizers (RDF) (NS1), 50% RDF + 25% recommended dose of nitrogen (RDN) through leaf compost (LC) + biofertilizer (NS2), 50% RDF + 25% RDN through vermicompost (VC) + biofertilizer (NS3)). The results revealed that diversification of rice–wheat system with legume (greengram) or vegetable (cabbage and onion) crops and integrated nutrient management strategies had positive effect on nutrient uptake and available nutrient status in the soil. Significantly higher uptake of N, P and K in all crops and Zn, Fe, Mn and Cu in rice and wheat were observed with NS2 and NS3 as compared to NS0. Available N, P and K status were significantly higher in NS2 and NS3 as against NS0 and NS1. Inclusion of cereal crops in the cropping systems showed a negative apparent N balance, but inclusion of vegetable crops in the cropping systems exhibited positive apparent N balance under different nutrient management strategies except NS0. The highest positive apparent N balance was observed in NS1 treatment. The apparent P balance was found to be positive in all the cropping systems with all the nutrients sources except NS0. Apparent K balance was found negative in all the cropping systems under different nutrient management strategies. Thus, cropping systems with summer greengram, cabbage and onion (CS2, CS3 and CS4) under integrated nutrient management practices (NS2 and NS3) were found more sustainable after two years of cropping cycle and can be advocated by the farmers of IGP.
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Isken, Olaf, Thomas Walther, Luis Wong-Dilworth, Dirk Rehders, Lars Redecke, and Norbert Tautz. "Identification of NS2 determinants stimulating intrinsic HCV NS2 protease activity." PLOS Pathogens 18, no. 6 (June 21, 2022): e1010644. http://dx.doi.org/10.1371/journal.ppat.1010644.

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Hepatitis C Virus NS2-NS3 cleavage is mediated by NS2 autoprotease (NS2pro) and this cleavage is important for genome replication and virus assembly. Efficient NS2-NS3 cleavage relies on the stimulation of an intrinsic NS2pro activity by the NS3 protease domain. NS2pro activation depends on conserved hydrophobic NS3 surface residues and yet unknown NS2-NS3 surface interactions. Guided by an in silico NS2-NS3 precursor model, we experimentally identified two NS2 surface residues, F103 and L144, that are important for NS2pro activation by NS3. When analyzed in the absence of NS3, a combination of defined amino acid exchanges, namely F103A and L144I, acts together to increase intrinsic NS2pro activity. This effect is conserved between different HCV genotypes. For mutation L144I its stimulatory effect on NS2pro could be also demonstrated for two other mammalian hepaciviruses, highlighting the functional significance of this finding. We hypothesize that the two exchanges stimulating the intrinsic NS2pro activity mimic structural changes occurring during NS3-mediated NS2pro activation. Introducing these activating NS2pro mutations into a NS2-NS5B replicon reduced NS2-NS3 cleavage and RNA replication, indicating their interference with NS2-NS3 surface interactions pivotal for NS2pro activation by NS3. Data from chimeric hepaciviral NS2-NS3 precursor constructs, suggest that NS2 F103 is involved in the reception or transfer of the NS3 stimulus by NS3 P115. Accordingly, fine-tuned NS2-NS3 surface interactions are a salient feature of HCV NS2-NS3 cleavage. Together, these novel insights provide an exciting basis to dissect molecular mechanisms of NS2pro activation by NS3.
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Anderson, Jenna, Emmanuel Bréard, Karin Lövgren Bengtsson, Kjell-Olov Grönvik, Stéphan Zientara, Jean-Francois Valarcher, and Sara Hägglund. "Purification, Stability, and Immunogenicity Analyses of Five Bluetongue Virus Proteins for Use in Development of a Subunit Vaccine That Allows Differentiation of Infected from Vaccinated Animals." Clinical and Vaccine Immunology 21, no. 3 (January 22, 2014): 443–52. http://dx.doi.org/10.1128/cvi.00776-13.

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ABSTRACTBluetongue virus (BTV) causes bluetongue disease, a vector-borne disease of ruminants. The recent northerly spread of BTV serotype 8 in Europe resulted in outbreaks characterized by clinical signs in cattle, including unusual teratogenic effects. Vaccination has been shown to be crucial for controlling the spread of vector-borne diseases such as BTV. With the aim of developing a novel subunit vaccine targeting BTV-8 that allows differentiation of infected from vaccinated animals, five His-tagged recombinant proteins, VP2 and VP5 of BTV-8 and NS1, NS2, and NS3 of BTV-2, were expressed in baculovirus orEscherichia coliexpression systems for further study. Optimized purification protocols were determined for VP2, NS1, NS2, and NS3, which remained stable for detection for at least 560 to 610 days of storage at +4°C or −80°C, and Western blotting using sera from vaccinated or experimentally infected cattle indicated that VP2 and NS2 were recognized by BTV-specific antibodies. To characterize murine immune responses to the four proteins, mice were subcutaneously immunized twice at a 4-week interval with one of three protein combinations plus immunostimulating complex ISCOM-Matrix adjuvant or with ISCOM-Matrix alone (n= 6 per group). Significantly higher serum IgG antibody titers specific for VP2 and NS2 were detected in immunized mice than were detected in controls. VP2, NS1, and NS2 but not NS3 induced specific lymphocyte proliferative responses upon restimulation of spleen cells from immunized mice. The data suggest that these recombinant purified proteins, VP2, NS1, and NS2, could be an important part of a novel vaccine design against BTV-8.
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Phan, Tung, Rudolf K. F. Beran, Christopher Peters, Ivo C. Lorenz, and Brett D. Lindenbach. "Hepatitis C Virus NS2 Protein Contributes to Virus Particle Assembly via Opposing Epistatic Interactions with the E1-E2 Glycoprotein and NS3-NS4A Enzyme Complexes." Journal of Virology 83, no. 17 (June 10, 2009): 8379–95. http://dx.doi.org/10.1128/jvi.00891-09.

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ABSTRACT The hepatitis C virus NS2 protein has been recently implicated in virus particle assembly. To further understand the role of NS2 in this process, we conducted a reverse genetic analysis of NS2 in the context of a chimeric genotype 2a infectious cell culture system. Of 32 mutants tested, all were capable of RNA replication and 25 had moderate-to-severe defects in virus assembly. Through forward genetic selection for variants capable of virus spread, we identified second-site mutations in E1, E2, NS2, NS3, and NS4A that suppressed NS2 defects in assembly. Two suppressor mutations, E1 A78T and NS3 Q221L, were further characterized by additional genetic and biochemical experiments. Both mutations were shown to suppress other NS2 defects, often with mutual exclusivity. Thus, several NS2 mutants were enhanced by NS3 Q221L and inhibited by E1 A78T, while others were enhanced by E1 A78T and inhibited by NS3 Q221L. Furthermore, we show that the NS3 Q221L mutation lowers the affinity of native, full-length NS3-NS4A for functional RNA binding. These data reveal a complex network of interactions involving NS2 and other viral structural and nonstructural proteins during virus assembly.
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Ilyas, Fizza, Muhammad Jamsahid, Irfan Bashir, Rabia Aslam, Tooba Mehboob, Naila Tabassam, and Muhammad Nadeem Alvi. "Solvent Diffusion Method: An Effective Approach to Formulate Nanosponges Loaded with Naproxen Sodium." RADS Journal of Pharmacy and Pharmaceutical Sciences 8, no. 2 (November 11, 2020): 74–80. http://dx.doi.org/10.37962/jpps.v8i2.338.

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Objective: Solubility of naproxen sodium is limited. In conventional dosage form it causes different gastro intestinal problems. To overcome these difficulties naproxen sodium loaded nano sponges were designed. Methodology: Nanosponges were formulated by using emulsion solvent evaporation technique. To obtain dispersion of nanosponges, homogenization of active drug, with specified quantities of polyvinyl alcohol, dichloromethane, ethyl cellulose and distilled, water was done. Compatibility among excipients and active drug was checked by FTIR and results didn’t show any interaction between them. 11 trial formulations were tested for poly dispersity, zeta potential, particle size and viscosity. Results: Results showed all formulations except NS9, NS10 and NS11 were in nano range. Formulation NS1 to NS6 fall in category of “mid poly dispersity” and formulation NS7 to NS11 were in the category of “very poly dispersity”. Values of Zeta potential of all formulations were in negative range -0.106 to -9.75 mV. The value of viscosity of all formulations were 0.8872. NS2 and NS3 were selected for further testing like Franz cell diffusion study, stability testing and drug loading efficiency. In Franz cell diffusion study, drug release for NS2= 89.62%, for NS3= 89.10% at 50 minutes’ time. Stability studies performed for the 21 days, NS2 and NS3 revealed slight change in percentage drug content at 4°C and 25°C, and major changes were observed at 45°C temperature. Drug loading efficiency was found in NS2= 97.659 % and for NS3= 98.901%. Conclusion: Nanosponges formulations loaded with naproxen sodium have successfully been prepared.
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Yi, MinKyung, Yinghong Ma, Jeremy Yates, and Stanley M. Lemon. "Compensatory Mutations in E1, p7, NS2, and NS3 Enhance Yields of Cell Culture-Infectious Intergenotypic Chimeric Hepatitis C Virus." Journal of Virology 81, no. 2 (November 1, 2006): 629–38. http://dx.doi.org/10.1128/jvi.01890-06.

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ABSTRACT There is little understanding of mechanisms underlying the assembly and release of infectious hepatitis C virus (HCV) from cultured cells. Cells transfected with synthetic genomic RNA from a unique genotype 2a virus (JFH1) produce high titers of virus, while virus yields are much lower with a prototype genotype 1a RNA containing multiple cell culture-adaptive mutations (H77S). To characterize the basis for this difference in infectious particle production, we constructed chimeric genomes encoding the structural proteins of H77S within the background of JFH1. RNAs encoding polyproteins fused at the NS2/NS3 junction (“H-NS2/NS3-J”) and at a site of natural, intergenotypic recombination within NS2 [“H-(NS2)-J”] produced infectious virus. In contrast, no virus was produced by a chimera fused at the p7-NS2 junction. Chimera H-NS2/NS3-J virus (vH-NS2/NS3-J) recovered from transfected cultures contained compensatory mutations in E1 and NS3 that were essential for the production of infectious virus, while yields of infectious vH-(NS2)-J were enhanced by mutations within p7 and NS2. These compensatory mutations were chimera specific and did not enhance viral RNA replication or polyprotein processing; thus, they likely compensate for incompatibilities between proteins of different genotypes at sites of interactions essential for virus assembly and/or release. Mutations in p7 and NS2 acted additively and increased the specific infectivity of vH-(NS2)-J particles, while having less impact on the numbers of particles released. We conclude that interactions between NS2 and E1 and p7 as well as between NS2 and NS3 are essential for virus assembly and/or release and that each of these viral proteins plays an important role in this process.
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Juhász, Evelin Kármen, and Andrea Balláné Kovács. "The effect of sulphur and nitrogen supply on the growth and nutrient content of spring wheat (Triticum aestivum L.)." Acta Agraria Debreceniensis, no. 74 (June 30, 2018): 65–70. http://dx.doi.org/10.34101/actaagrar/74/1666.

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Sulphur is an essential element for plants. Decreasing sulphur deposition from the air, and the use of more concentrated phosphate fertilizers, which contain no sulphur, has led to reports of sulphur deficiencies for wheat. Sulphur deficiency significantly affects yield and also the quality of wheat. The pot experiment was set up on calcareous chernozem soil at Látókép, Hungary, test plant was spring wheat (Triticum aestivum L). Seven treatments were used where nitrogen and sulphur were supplied as soil fertilizers in increasing rates (NS1, NS2, NS3) and in foliar fertilizer as well (NS1+fol., NS2+fol., NS3+fol.). Plant aboveground biomass production was determined in samples taken in the stages of development BBCH 29-30, 51-59, 61-69, 89. The nitrogen and sulphur content of straw and grain were measured. N/S ratios of grain and straw were calculated. The weights of grain were ranging between 8.6–16.1 g/pot. NS2 and NS2+fol. treatments produced the highest values. Foliar fertilizer had no further effect on grain. Analysing the values of the straw, it was observed that tendencies were similar to values of grain. The NS2 treatment produced the highest weight of straw and the NS3 rate already decreased that amount. The obtained results show the unfavourable effect of excessively high rate applied in NS3 treatment. The supplementary foliar fertilizer had no significant influence on the weight of straw. Both N and S-uptake of plant was very intensive at the stem elongation stage, then the N and S-content of plant continuously decreased in time in all treatments. The N-content of grain ranged between 2.215–2.838%. The N-content of grain slightly increased with increasing of nitrogen doses. In the higher doses (NS2, NS3) foliar fertilization slightly increased the nitrogen content of grain, although this effect was not statistically proved. The N-content of straw varied from 0.361 to 0.605%. The growing dose of soil fertilizer also considerably increased the nitrogen content of straw. Foliar fertilization further increased the nitrogen content of straw. The S-content of grain ranged between 0.174–0.266%. The lowest fertilizer dose (NS1) significantly increased the sulphur content of grain. The further increasing fertilizer doses (NS2, NS3) did not cause additional enhance in sulphur content of grain. The foliar fertilizer also did not change the sulphur value of plant. The increasing amount of soil fertilizer and the supplementary foliar fertilizer had no effect on the sulphur content of straw. The treatments influenced the N/S ratios of grain and straw. On the basis of experimental results it can be concluded that the examined nitrogen and sulphur containing soil fertilizer had positive effect on the growth and yield of spring wheat grown on the calcareous chernozem soil. The soil fertilizer application enhanced the grain nitrogen and sulphur content. The highest rate of fertilizer (600 kg ha-1) proved to have decreasing effect on the yield. The sulphur and nitrogen containing foliar fertilizer did not have significant effect on the yield parameters but slightly increased the nitrogen content of plant.
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Klemens, O., D. Dubrau, and N. Tautz. "Characterization of the Determinants of NS2-3-Independent Virion Morphogenesis of Pestiviruses." Journal of Virology 89, no. 22 (September 9, 2015): 11668–80. http://dx.doi.org/10.1128/jvi.01646-15.

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ABSTRACTA peculiarity of theFlaviviridaeis the critical function of nonstructural (NS) proteins for virus particle formation. For pestiviruses, like bovine viral diarrhea virus (BVDV), uncleaved NS2-3 represents an essential factor for virion morphogenesis, while NS3 is an essential component of the viral replicase. Accordingly, in natural pestivirus isolates, processing at the NS2-3 cleavage site is not complete, to allow for virion morphogenesis. Virion morphogenesis of the related hepatitis C virus (HCV) shows a major deviation from that of pestiviruses: while RNA replication also requires free NS3, virion formation does not depend on uncleaved NS2-NS3. Recently, we described a BVDV-1 chimera based on strain NCP7 encompassing the NS2-4B*-coding region of strain Osloss (E. Lattwein, O. Klemens, S. Schwindt, P. Becher, and N. Tautz, J Virol 86:427–437, 2012, doi:10.1128/JVI.06133-11). This chimera allowed for the production of infectious virus particles in the absence of uncleaved NS2-3. The Osloss sequence deviates in the NS2-4B* part from NCP7 in 48 amino acids and also has a ubiquitin insertion between NS2 and NS3. The present study demonstrates that in the NCP7 backbone, only two amino acid exchanges in NS2 (E1576V) and NS3 (V1721A) are sufficient and necessary to allow for efficient NS2-3-independent virion morphogenesis. The adaptation of a bicistronic virus encompassing an internal ribosomal entry site element between the NS2 and NS3 coding sequences to efficient virion morphogenesis led to the identification of additional amino acids in E2, NS2, and NS5B that are critically involved in this process. The surprisingly small requirements for approximating the packaging schemes of pestiviruses and HCV with respect to the NS2-3 region is in favor of a common mechanism in an ancestral virus.IMPORTANCEFor positive-strand RNA viruses, the processing products of the viral polyprotein serve in RNA replication as well as virion morphogenesis. For bovine viral diarrhea virus, nonstructural protein NS2-3 is of critical importance to switch between these processes. While free NS3 is essential for RNA replication, uncleaved NS2-3, which accumulates over time in the infected cell, is required for virion morphogenesis. In contrast, the virion morphogenesis of the related hepatitis C virus is independent from uncleaved NS2-NS3. Here, we demonstrate that pestiviruses can adapt to virion morphogenesis in the absence of uncleaved NS2-3 by just two amino acid exchanges. While the mechanism behind this gain of function remains elusive, the fact that it can be achieved by such minor changes is in line with the assumption that an ancestral virus already used this mechanism but lost it in the course of adapting to a new host/infection strategy.
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Yen, Yu-Ting, and Betty Wu-Hsieh. "Dengue viral component triggering reactive nitrogen and oxygen species production leads to endothelial cell apoptosis (45.33)." Journal of Immunology 182, no. 1_Supplement (April 1, 2009): 45.33. http://dx.doi.org/10.4049/jimmunol.182.supp.45.33.

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Abstract Vascular leakage is one of the life-threatening complications occurring in dengue patients, however, the pathogenic mechanism is not well understood. In dissecting the complex interplay between the virus and the host, we found that dengue virus productively infects the endothelial cells and causes apoptotic cell death through triggering the production of reactive nitrogen (RNS) and oxygen radicals (ROS). To determine the dengue virus component(s) that is responsible for the cause of cell death, we transfected human microvascular endothelial cell HMEC with pCR3.1 plasmids encoding Flag-tagged viral components Core, PrM, NS1, NS2A, NS2B, NS3, NS2B-NS3, NS4A, NS4B, and NS5, separately. Apoptotsis in cells transfected with NS2B-NS3 was 10-fold higher and that in cells transfected with NS1, NS3 and NS5 or core was only between 4- to 6-fold higher than cells transfected with vector alone. Confocal microscopy revealed that while NS2B expression remained in the cytosol, NS3 and NS2B-NS3 in both the cytosol and the nucleus at 24 h after transfection. Interestingly, the effect of NS2B-NS3 on endothelial cells was reversed in the presence of NO and ROS inhibitors. These results demonstrate that NS2B-NS3, being the major component that induces endothelial cell apoptosis, interacts with subcellular components in both the cytosol and the nucleus, and induces the production of RNS and ROS to cause apoptotic outcome.
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Kümmerer, Beate M., Dieter Stoll, and Gregor Meyers. "Bovine Viral Diarrhea Virus Strain Oregon: a Novel Mechanism for Processing of NS2-3 Based on Point Mutations." Journal of Virology 72, no. 5 (May 1, 1998): 4127–38. http://dx.doi.org/10.1128/jvi.72.5.4127-4138.1998.

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ABSTRACT Bovine viral diarrhea virus (BVDV) isolates can either be cytopathogenic (cp) or noncytopathogenic (noncp). While both biotypes express the nonstructural protein NS2-3, generation of NS3 strictly correlates with the cp phenotype. The production of NS3 is usually caused by cp specific genome alterations, which were found to be due to RNA recombination. Molecular analyses of the cp BVDV strain Oregon revealed that it does not possess such genome alterations but nevertheless is able to generate NS3 via processing of NS2-3. The NS3 serine protease is not involved in this cleavage, which, according to protein sequencing, occurs between amino acids 1589 and 1590 of the BVDV Oregon polyprotein. Transient-expression studies indicated that important information for the cleavage of NS2-3 is located within NS2. This was verified by expression of chimeric constructs containing cDNA fragments derived from BVDV Oregon and a noncp BVDV. It could be shown that the C-terminal part of NS2 plays a crucial role in NS2-3 cleavage. These data, together with results obtained by site-specific exchanges in this region, revealed a new mechanism for NS2-3 processing which is based on point mutations within NS2.
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Dissertations / Theses on the topic "Ns3"

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Oliveira, Anibal Silva de. "Clonagem e expressão das proteínas recombinantes NS1 e NS3 do vírus da dengue tipo 3." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-21062013-141504/.

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A dengue é uma doença infecciosa com grandes taxas de morbimortalidade, causada pelo vírus da dengue (DENV). Segundo a Organização Mundial de Saúde, cerca de 50 a 100 milhões de pessoas são infectadas anualmente em mais de 100 países tropicais e subtropicais de todos os continentes. O espectro clínico da infecção pelo DENV pode incluir formas assintomáticas ou sintomaticas que variam desde uma febre indeterminada e autolimitada, passando pela febre clássica da dengue (FD) até quadros graves denominados febre hemorrágica da dengue/síndrome do choque da dengue (FHD/SCD). Recentemente, ocorreu um dramático aumento do número de casos de FHD/SCD nas Américas, e este aumento coincidiu com a introdução do dengue sorotipo 3, genótipo III. No presente trabalho, objetivou-se a clonagem e a expressão das proteínas NS1 e NS3 do vírus da dengue tipo 3. As proteínas NS1 e NS3 do DENV-3 foram clonadas e expressas com sucesso em sistema procarioto. A amplificação dos genes das proteínas NS1 e NS3 foi realizada por RT-PCR, o qual gerou amplicons de cerca de 1050 e 1850 pb, respectivamente. Em seguida, os genes foram clonados por inserção dos amplicons no vetor plasmidial pCR-XL. Os genes de NS1 e NS3 foram subclonados no vetor de expressão pQE-30 através de sítios de restrição para as enzimas BamHI e HindIII. A expressão proteica foi obtida em sistema procarioto utilizando a cepa BL21(DE3) de E. coli, resultando em proteínas de 45 e 70 kDa as quais foram confirmadas por análises em Western blot utilizando como anticorpo primário fluido ascítico imune de camundongos e soro de pacientes com dengue. Estas proteínas virais podem ser utilizadas para estudos relacionados à patogênese, replicação e mecanismos de escape do sistema imune do DENV, além disso, podem ser potencias antígenos em métodos de diagnóstico.
Dengue is an infectious disease with high morbidity and mortality rates caused by dengue virus (DENV). According to the World Health Organization, about 50 to 100 million people are infected annually in more than 100 tropical and subtropical countries from all continents. The clinical spectrum of DENV infection can includes asymptomatic or symptomatic forms ranging from undetermined and self-limited fever, through dengue fever (DF) to severe disease called dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). Recently, there has been a dramatic increase in the number of cases of DHF/DSS in the Americas, and this increase coincided with the introduction of dengue virus type 3 (DENV-3), genotype III. The present study aimed to clone and express NS1 and NS3 proteins of DENV-3. The NS1 and NS3 proteins of DENV-3 was successfully cloned and expressed in a prokaryotic system. Amplification of NS1 and NS3 genes was carried out by RT-PCR, which yielded amplicons of approximately 1050 and 1850 bp, respectively. Then, the genes were cloned by inserting the amplicons into the plasmid vector pCR-XL. NS1 and NS3 genes were subcloned into the expression vector pQE-30 through the restriction sites for BamHI and HindIII enzymes. The protein expression was obtained in a prokaryotic system using the strain BL21 (DE3) of E. coli, resulting in 45 and 70 kDa proteins, which were confirmed by Western blot analysis using immune mouse ascitic fluid and serum of patients with dengue as primary antibody. These viral proteins can be used to study the pathogenesis, mechanisms of replication and immune escape of DENV, moreover, can be potential antigens in diagnostic methods.
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Gallivan, John-Paul. "Biochemical characteristics of Powassan virus NS3 and analysis of antibody-based inhibitors of HCV NS3." Thesis, Imperial College London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.409695.

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Bureš, František. "Nové laboratorní úlohy v prostředí NS3." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-361719.

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Cílem bylo navrhnout dva simulační scénáře v prostředí NS-3. První scénář obsahuje ARQ (Automatic Repeat Request) metody v TCP (Transmission Control Protocol). Je v něm porovnání Stop-and-Wait, Go-Back-N a Selective-Repeat metod. Teoretická část obsahuje TCP a ARQ. Druhý scénář je o způsobech přenosu zpráv. Vytvořený scénář převážně s komutací paketů a buněk a teoretické základy jsou obsaženy v práci. Je v něm porovnání metod komutací s různou velikostí paketu/buňky, počtem uzlů a důsledek zpoždění v každé metodě. Scénáře jsou ve formě laboratorní úlohy s instrukcemi k vypracování.
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Brand, Carolin. "In-depth characterization of the NS3:NS5 interaction within the West Nile virus replicase complex during positive strand RNA synthesis." Mémoire, Université de Sherbrooke, 2017. http://hdl.handle.net/11143/10487.

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Les Flavivirus transmis par les moustiques comme le virus du Nil occidental, le virus de la dengue, le virus de la fièvre jaune, le virus de l’encéphalite japonaise et le virus Zika constituent des préoccupations croissantes de santé publique. Ils se sont répandus dans le monde au cours des dernières décennies, et les épidémies sont devenues plus fréquentes et plus sévères. Chaque année, des millions de personnes sont infectées et environ 50 000 patients décèdent d’infections à Flavivirus. Malgré les nombreux efforts de recherche, il n’y a actuellement aucun médicament antiviral spécifique disponible, et des nouvelles stratégies antivirales sont indispensables. Comprendre comment les Flavivirus fonctionnent au niveau moléculaire aidera à découvrir des nouvelles cibles pour l'intervention thérapeutique. Les Flavivirus ont un génome d'ARN simple brin de polarité positive qui code pour trois protéines structurales et huit protéines non structurales. Seules deux des huit protéines non structurales ont des activités enzymatiques. NS3 possède un domaine protéase et un domaine hélicase, et NS5 a un domaine méthyl- et guanylyltransférase et un domaine ARN polymérase ARN-dépendante. Ensemble, ils répliquent le génome viral. Ici, nous caractérisons l'interaction entre NS3 et NS5 dans le complexe de réplication du virus du Nil occidental pendant la synthèse d’ARN de polarité positive. Un modèle d'interaction comprenant NS3, NS5 et l’ARN viral a été développé basé sur des structures cristallines connues ainsi que des activités enzymatiques des deux protéines individuelles, et ce modèle a été soumis à des simulations de dynamique moléculaire. Les interactions potentielles entre les protéines NS3 et NS5 ont été identifiées. Les résidus impliqués dans ces interactions ont été mutés dans un réplicon du virus du Nil occidental et les effets de ces mutations sur la réplication virale ont été évalués. Une région particulière à la surface de la protéine NS3 a été identifiée comme étant cruciale pour la réplication virale, très probablement parce qu'elle interagit avec NS5. Cette région pourrait être une cible attrayante pour la recherche de composés qui pourraient interférer avec l'interaction entre NS3 et NS5 et donc posséder un potentiel antiviral intéressant.
Abstract : Mosquito-borne Flaviviruses like West Nile virus, Dengue virus, Yellow Fever virus, Japanese encephalitis virus, and Zika virus are increasing public health concerns. They have spread globally during the past decades, and outbreaks have recently become more frequent and more severe. Every year, millions of people are infected, and approximately 50,000 patients die from Flavivirus infections. Despite extensive research efforts, there are currently no specific antiviral drugs available, and new antiviral strategies are greatly needed. Understanding how Flaviviruses work on a molecular level will help in uncovering new points for therapeutic intervention. Flaviviruses have a single-stranded RNA genome of positive polarity that encodes three structural and eight non-structural proteins. Only two of the eight non-structural proteins have enzymatic activities. NS3 has an N-terminal protease domain and a C-terminal helicase domain, and NS5 has an N-terminal capping enzyme domain and a C-terminal RNA-dependent RNA polymerase domain. Together, they replicate the viral genome. Here we characterize the NS3:NS5 interaction within the West Nile virus RNA replicase complex during positive strand synthesis. An interaction model including NS3, NS5 and viral RNA was developed based on the known crystal structures as well as enzymatic activities of the two individual proteins, and this model was subjected to molecular dynamics simulations. Potential interactions between the NS3 and NS5 proteins were identified. Residues involved in these interactions were mutated in a West Nile virus replicon, and the effects of these mutations on viral replication were evaluated. One particular region on the surface of the NS3 protein was identified to be crucial for viral replication, most likely because it mediates the interaction with NS5. This region might be an attractive target for the search of compounds that could interfere with the NS3:NS5 interaction and therefore possess an interesting antiviral potential.
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Evans, Julianna Marie. "Spacecraft-ns3: Spacecraft Discrete-Event Network Simulation." Thesis, Virginia Tech, 2020. http://hdl.handle.net/10919/99102.

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As near-Earth space becomes more populated with large constellations of satellites and research into spacecraft autonomy and disaggregation becomes more prevalent, it will be increasingly important to design effective communication procedures between satellites to efficiently share resources and avoid collisions. Though there have been several space networking simulation tools created in recent years, they all lack rigorous astrodynamics models or use high-fidelity but bulky and computationally taxing commercial software. This research presents Spacecraft-ns3, an extension to the ns-3 network simulator. Using a modular approach, Spacecraft-ns3 propagates orbit state, plans discrete events, and analyzes network metrics and flows. A case study using Spacecraft-ns3 is presented for exploratory space network analysis.
Master of Science
Near-Earth space has become more crowded in recent years due to the increasing number of large constellations of satellites in this region. Autonomous vehicle research has been applied to Earth satellites primarily to share power and computing resources between satellites, or to prevent collisions between satellites. Both of these factors require effective communication procedures between satellites, which can be inexpensively simulated with network simulators. However, network simulators are primarily designed for ground-based use, and must be combined with an astrodynamics simulator to effectively simulate satellite networks. This research presents Spacecraft-ns3, an integrated simulator that defines spacecraft orbits and attitude, and analyzes network activity. This simulator improves upon prior simulation efforts by extending the ns-3 network simulator with efficient and high-fidelity astrodynamics models. The Spacecraft-ns3 simulator is demonstrated in an exploratory case study.
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Štefančík, Matej. "Návrh laboratorních úloh v simulačním prostředí NS3." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-220382.

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Diploma thesis provides an introduction to the ns-3 network simulator. It describes the basic abstactions and components of the instrument. This work further discusses the theory, which was applied during simulation creation. The concept of unicast, the issue of IP addressing and routing in networks are specificaly analyzed. The practical part is focused on the creation of four laboratory tasks within the scope of Advanced Communication Techniques. The first task is dedicated to the the issue of static unicast routing in the network. It clarifies the impact of metrics on the process of routing. The following task compares the different types of ICMPv4 and ICMPv6 messages. The third task discusses additional functions of the ICMPv6. Specifically, it focuses on five different types of ICMPv6 messages defined by NDP (Neighbor Discovery Protocol) and their role in the network traffic. The last task focuses on ensuring the quality of services (QoS) in wireless networks, by setting priorities and thereby dividing the traffic into different classes. Attention is focused on parameters such as throughput, delay and jitter.
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Kolář, Jan. "Simulátor přenosových funkcí silnoproudého vedení v NS3." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-220401.

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This thesis deals with the powerline communication focusing on the simulations of channel transfer functions. The introduction summarizes basic information about PLC followed by the analysis of the available PLC Software simulator (implemented in NS-3) and the analysis of implemented noises and interferences and its extensions. One chapter is devoted to possibilities of link capacity calculation. Within the thesis, various methods of calculation of primary parameters of transmission line were implemented and the simulator was compared with other freely available simulators. Subsequently, simulations were carried out focused on channel transfer functions in different topologies, on the impedance changes of branch, on the lengths of branch, and changes in the direct path between communicating nodes.
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Costa, Simone Morais da. "Vacinas de DNA contra o vírus da dengue utilizando como antígenos as proteínas NS1 e NS3." reponame:Repositório Institucional da FIOCRUZ, 2008. https://www.arca.fiocruz.br/handle/icict/12179.

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Made available in DSpace on 2015-11-11T12:09:49Z (GMT). No. of bitstreams: 2 simone_costa_ioc_dout_2008.pdf: 34704570 bytes, checksum: e34eaf901d373dd2f63e3ffbecbb7e50 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2015-04-14
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
O vírus da dengue (DENV) consiste de quatro sorotipos antigenicamente relacionados: DENV-1, DENV-2, DENV-3 e DENV-4. Apesar dos diversos esforços para o desenvolvimento de uma vacina contra dengue, ainda não há nenhuma comercialmente disponível. As proteínas não estruturais 1 e 3 (NS1 e NS3) são indicadas como antígenos promissores para o desenvolvimento de uma vacina contra DENV. Segundo alguns estudos, a proteína NS1 é capaz de induzir uma resposta protetora de anticorpos com atividade de fixação do complemento. A proteína NS3, que realiza reações enzimáticas essenciais para a replicação viral, parece ser imunogênica, contendo um predomínio de epítopos para linfócitos T CD4+ e CD8+. No presente trabalho nós avaliamos o potencial de vacinas de DNA baseadas nas proteínas NS1 e NS3 de DENV-2. Foram construídos cinco plasmídeos, pcTPANS3, pcTPANS3H, pcTPANS3P, pcTPANS3N e pcTPANS3C, contendo a seqüência que codifica o peptídeo sinal do ativador de plasminogênio de tecido humano (t-PA) fusionado ao gene NS3 inteiro ou partes destes. Todos estes plasmídeos mediaram a expressão das proteínas recombinantes in vitro em células eucarióticas Camundongos foram inoculados com estes plasmídeos e desafiados com DENV-2 por via intracerebral (i.c.). Nenhuma destas construções induziu níveis satisfatórios de proteção. Além dos plasmídeos com NS3, foram construídas quatro vacinas de DNA baseadas no gene NS1: 1 - pcENS1, que codifica a região C-terminal da proteína E fusionada à NS1, 2 - pcENS1ANC, similar ao pcENS1 com a adição da porção N-terminal da NS2A (ANC), 3 - pcTPANS1, que codifica o peptídeo sinal t-PA fusionado à NS1 e 4 - pcTPANS1ANC, semelhante ao pcTPANS1 com a adição da seqüência ANC. A proteína NS1 recombinante foi detectada nos extratos celulares e sobrenadante das culturas de células BHK transfectadas com pcTPANS1, pcENS1 e pcENS1ANC. Tais resultados indicam que as seqüências sinais t-PA e E direcionaram a NS1 para secreção. A proteína NS1 também foi observada associada à membrana plasmática de células transfectadas com pcENS1ANC, demonstrando a importância da seqüência ANC para o seu ancoramento. Todos os camundongos imunizados com pcTPANS1 ou pcENS1 produziram altos níveis de anticorpos, direcionados principalmente para epítopos conformacionais da NS1, enquanto que somente metade dos animais inoculados com pcENS1ANC apresentaram níveis detectáveis de anticorpos A resposta de anticorpos se mostrou duradoura (até 56 semanas após a primeira dose das vacinas), e os animais apresentaram uma rápida resposta secundária após um reforço de DNA. Camundongos imunizados com os plasmídeos pcTPANS1 e pcENS1 se mostraram protegidos contra desafios com DENV-2 por via i.c., sendo o pcTPANS1 levemente mais protetor. Estes dois plasmídeos ativaram a produção de diferentes subclasses de IgG específicas contra NS1. Não foi observada proteção interespecífica quando camundongos imunizados com pcTPANS1 foram desafiados por via i.c. com DENV-1. Os animais imunizados com o pcTPANS1 foram desafiados com DENV-2 por via intraperitoneal e também se mostraram protegidos. Neste modelo de desafio, foi observada uma diminuição dos efeitos histopatológicos do vírus no fígado dos animais vacinados. Resultados preliminares sugerem à lise de células infectadas com DENV-2, dependente do complemento, na presença dos anticorpos direcionados contra NS1
Dengue virus (DENV) consists of four antigenically related serotypes: DENV-1, DENV-2, DENV-3 and DENV-4. Although considerable research has been conducted towards the development of a DENV vaccine, no vaccine is yet commercially available. The non-structural proteins 1 and 3 (NS1 and NS3) have been identified as promising antigens for the development of vaccines against DENV. According to some reports, NS1 can elicit a protective antibody response with complement-fixing activities. NS3, a protein that carries out enzymatic reactions essential for viral replication, appears to be immunogenic, presenting a preponderance of the CD4+ and CD8+ T cell epitopes. In the present work we investigate the potential of DNA vaccines based on the DENV-2 NS1 and NS3 proteins. We constructed five recombinant plasmids, pcTPANS3, pcTPANS3H, pcTPANS3P, pcTPANS3N and pcTPANS3C, which contain the sequence that codes the signal peptide derived from the human tissue plasminogen activator (t-PA) fused to the full or partial length of the DENV-2 NS3 gene. Results indicated that these plasmids promoted the expression of recombinant proteins in eukaryotic cells. Mice were inoculated with these plasmids and challenged by the intracerebral (i.c.) route with DENV-2. None of these constructs induced acceptable protection. Moreover, we constructed four DNA vaccines based on the DENV-2 NS1 gene: 1 - pcENS1, coding the C-terminal of the E protein fused to NS1, 2 - pcENS1ANC, similar to pcENS1 with the addition of the N-terminal of NS2A (ANC), 3 - pcTPANS1, coding the t-PA signal sequence fused to NS1 and 4 - pcTPANS1ANC, similar to pcTPANS1 with the addition of the ANC sequence. The recombinant NS1 protein was detected in cell extracts and culture supernatants from pcTPANS1-, pcENS1- and pcENS1ANC-transfected BHK cells. Such results indicated that the E and t-PA sequences targeted NS1 to secretion. NS1 was also observed in association with plasma membrane of pcENS1ANC-transfected cells, which demonstrated the importance of the ANC sequence for cell anchoring. High levels of antibodies, mainly recognizing surface-exposed conformational epitopes of NS1, were induced in all mice immunized with pcTPANS1 and pcENS1, while only half of pcENS1ANC-inoculated animals presented detectable antibody levels. Long-term antibody response was observed in pcTPANS1 and pcENS1 immunized animals (56 weeks after the first vaccine inoculation) and there was a rapid secondary response after a DNA booster. Protection was elicited in pcTPANS1- and pcENS1-immunized mice challenged with DENV- 2 by the i.c. route and the pcTPANS1 seemed to generate a slightly higher protection. Moreover, these two plasmids induced different NS1-specific IgG subclasses. No protection was displayed when pcTPANS1-immunized animals were i.c. challenged with DENV-1. Animals inoculated with pcTPANS1 were also protected when they were challenged with DENV-2 by the intraperitoneal route. Liver tissue from vaccinated animals presented a remarkable decrease of hepatic damages in this challenge mouse model. Preliminary results suggested the complement-mediated lyses of DENV-2 infected cells in the presence of the NS1-specific antibody.
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Milhas, Sabine. "Développement d'outils pour l'étude des interactions protéine-protéine." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4020.

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Au cours de ma thèse je me suis intéressée aux interactions protéine-protéine (PPI’s). Les PPI’s jouent un rôle majeur dans une grande diversité de processus cellulaires et sont maintenant considérées comme une cible majeure dans le but de développer de nouveaux médicaments. Cependant, cibler ce type d’interactions requiert le développement de chimiothèques dédiées, permettant d’accélérer la découverte de molécules « touches ». Pour surmonter ce problème, une chimiothèque orientée PPI (2P2I3D) a été conçu au laboratoire. Dans un premier temps, j’ai donc évalué cette chimiothèque sur différents complexes possédant des interfaces variées. Les résultats obtenus ont révélé des taux de touches supérieurs à ceux obtenus avec des chimiothèques non orientées, de 0,2 à 1,6% contre 0,01 à 0,1%, respectivement. Cette étude a permis d’établir une preuve de concept de la faisabilité de créer une chimiothèque orientée PPI, permettant ainsi une accélération de la découverte de composés biologiquement actifs.Dans un deuxième temps, je me suis intéressée à l’interaction entre deux protéines majeures du virus de la dengue : les protéines NS3 et NS5. J’ai tout d’abord identifié et caractérisé un nouveau site d’interaction, ce qui m’a permis de mettre en évidence que cette interaction avait pour conséquence d’augmenter l’activité enzymatique du domaine hélicase. J’ai par la suite recherché et identifié des petites molécules chimiques capable d’inhiber cette interaction. Les différentes caractérisations effectuées ont permis de mettre en évidence un effet antiviral. Ces inhibiteurs constituent un excellent point de départ afin d’étudier plus en détail le rôle biologique de ce complexe
In my thesis I became interested in protein-protein interactions (PPI's). PPI's play a major role in a variety of cellular processes and are now considered a major target in order to develop new drugs. However, targeting such interactions requires the development of dedicated libraries, to accelerate the discovery of “hits”molecules .To overcome this issue, a focused chemical library PPI (2P2I3D) was designed in the laboratory.At first, I evaluated this chemical library on different complexes with diverse interfaces. The results showed higher hit rate to those obtained with non-oriented libraries, from 0.2 to 1.6% against 0.01 to 0.1%, respectively. This study has established a proof of concept of the feasibility of creating a focused chemical library PPI, thus accelerating the discovery of biologically active compounds.Secondly, I am interested in the interaction between two major proteins of dengue virus: the NS3 and NS5 proteins. I initially identified and characterized a novel interaction site, which allowed me to demonstrate that this interaction had the effect of increasing the enzymatic activity of the helicase domain. I searched and identified small molecules able to inhibit this interaction. The different characterizations helped to highlight an antiviral effect. These inhibitors are an excellent starting point to further explore the biological role of this complex
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Motyčka, Jan. "Implementace mechanismů zajišťujících “RAN Slicing” v simulačním nástroji Network Simulator 3." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2021. http://www.nusl.cz/ntk/nusl-442360.

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This thesis deals with the topic of network slicing technology in 5G networks, mainly on the RAN part. In the theoretical part, basic principles of 5G network slicing in core network part and RAN part are presented. Practical part contains a simulation scenario created in NS3 simulator with LENA 5G module. Results of this simulation are presented and discussed with the emphasis on RAN slicing.
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Books on the topic "Ns3"

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Pusat Informatik Untuk Pengelolaan Pendidikan dan Kebudayaan (Indonesia), ed. Memantapkan nomor statistik sekolah (NSS) dan menyusun nomor statistik bangunan (NSB) sekolah dasar, 1983. Jakarta: Departemen Pendidikan dan Kebudayaan, Badan Penelitian dan Pengembangan Pendidikan dan Kebudayaan, Pusat Informatik Untuk Pengelolaan Pendidikan dan Kebudayaan, 1987.

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NH3. London: Rickshaw, 2013.

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Evans, Johanna. Characterisation of the NS1 and the NS2 non-structural protein genes of human respiratory syncytial virus (HRSV). [s.l.]: typescript, 1994.

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Pusat Informatik Untuk Pengelolaan Pendidikan dan Kebudayaan (Indonesia), ed. Laporan tahap II NSS-NSB 1983 untuk persiapan wajib belajar, perhitungan alokasi ruang kelas baru sekolah dasar. [Jakarta]: Departemen Pendidikan dan Kebudayaan, Badan Penelitian dan Pengembangan Pendidikan dan Kebudayaan, Pusat Informatik untuk Pengelolaan Pendidikan dan Kebudayaan, 1987.

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Laul, Ulev. Kodumaast: NSV Liidu Ja Eesti NSV Riigihumnist. Tallinn: Eesti Raamat, 1988.

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Mastering NSS physics. Hong Kong: Radian Publishing Co., 2010.

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Heinsalu, Ülo. Eesti NSV koopad. Tallinn: "Valgus", 1987.

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Flint, Wendy. NSF development framework. Leicester: National Youth Agency, 2003.

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Vello, Tarmisto, ed. NSV liit: Teatmik. Tallinn: "Eesti Raamat", 1987.

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Vanatoa, Endel. Eesti NSV, teatmik. Tallinn: Perioodika, 1988.

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Book chapters on the topic "Ns3"

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Tay, Moon Y. F., and Subhash G. Vasudevan. "The Transactions of NS3 and NS5 in Flaviviral RNA Replication." In Advances in Experimental Medicine and Biology, 147–63. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8727-1_11.

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Liverton, Nigel J. "Evolution of HCV NS3/4a Protease Inhibitors." In Topics in Medicinal Chemistry, 231–59. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/7355_2018_39.

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van Staden, V., C. C. Smit, M. A. Stoltz, F. F. Maree, and H. Huismans. "Characterization of two African horse sickness virus nonstructural proteins, NS1 and NS3." In African Horse Sickness, 251–58. Vienna: Springer Vienna, 1998. http://dx.doi.org/10.1007/978-3-7091-6823-3_22.

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He, Yu, Jiang Wu, Jiaxin Li, and Zhanbo Xu. "NS3 Based Simulation Framework for 5G-IoV Networks." In Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering, 233–42. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-92511-6_15.

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Krawczyk, Mariusz, Anna Stankiewicz-Drogoń, Anne-Lise Haenni, and Anna Boguszewska-Chachulska. "Fluorometric Assay of Hepatitis C Virus NS3 Helicase Activity." In Methods in Molecular Biology, 211–21. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-355-8_15.

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Cheng, Wei. "Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers." In Methods in Molecular Biology, 229–55. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2214-7_15.

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Kwong, A. D., J. L. Kim, and C. Lin. "Structure and Function of Hepatitis C Virus NS3 Helicase." In Current Topics in Microbiology and Immunology, 171–96. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59605-6_9.

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Pathan, Mahabub Subhani, and K. Annapurna. "Simulation of Topology Based VANET Routing Protocols Using NS3." In Pervasive Computing and Social Networking, 633–42. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-2840-6_48.

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Abdelhakim, Boudhir Anouar, Ben Ahmed Mohamed, Abbadi Aya, Achahbar Salma, and Soufiani Assia. "Performance Evaluation of NS2 and NS3 Simulators Using Routing Protocols in Mobile Ad Hoc Networks." In Lecture Notes on Data Engineering and Communications Technologies, 372–85. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-15191-0_36.

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McCauley, John A., and Michael T. Rudd. "The Invention of Grazoprevir: An HCV NS3/4a Protease Inhibitor." In Topics in Medicinal Chemistry, 355–87. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/7355_2018_41.

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Conference papers on the topic "Ns3"

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Yin, Hao, Pengyu Liu, Keshu Liu, Liu Cao, Lytianyang Zhang, Yayu Gao, and Xiaojun Hei. "ns3-ai." In WNS3 2020: 2020 Workshop on ns-3. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3389400.3389404.

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Zubow, Anatolij, Christos Laskos, and Falko Dressler. "FTM-ns3: WiFi Fine Time Measurements for NS3." In 2022 17th Wireless On-Demand Network Systems and Services Conference (WONS). IEEE, 2022. http://dx.doi.org/10.23919/wons54113.2022.9764460.

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Hiremath, Mrutyunjay G., Jayalakshmi G. Naragund, Nitya N. Kulkarni, and R. M. Banakar. "Enhancing NS3 for MRMC WMN." In 2015 International Conference on Emerging Research in Electronics, Computer Science and Technology (ICERECT). IEEE, 2015. http://dx.doi.org/10.1109/erect.2015.7499005.

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Fonseca, António, Camões André, and Teresa Vazão. "Geographical routing implementation in NS3." In Fifth International Conference on Simulation Tools and Techniques. ACM, 2012. http://dx.doi.org/10.4108/icst.simutools.2012.247688.

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Cadiz, Jewel Mae S., Christine Joy U. Susana, Marla A. Endriga, and Enrique Jose L. Frio. "Molecular Docking of Traditional Chinese Medicinal Compounds Against Dengue Virus NS3 Protease and NS3 Helicase." In 2020 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2020. http://dx.doi.org/10.1109/bibm49941.2020.9312980.

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Halder, Rishav, Sridhar Mundra, Uttaran Dey, Sreejita Ghosh, Sreeja Karmakar, and Raja Karmakar. "NS3TCG: NS3 Topology and Code Generator." In 2018 International Conference on Recent Innovations in Electrical, Electronics & Communication Engineering (ICRIEECE). IEEE, 2018. http://dx.doi.org/10.1109/icrieece44171.2018.9008653.

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Barnes, Jr., Peter, James Brase, Thomas Canales, Matthew Damante, Matthew Horsley, David Jefferson, and Ron Soltz. "A Benchmark Model for Parallel ns3." In Fifth International Conference on Simulation Tools and Techniques. ACM, 2012. http://dx.doi.org/10.4108/icst.simutools.2012.247778.

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Addie, Ronald, and Joshua Natarajan. "Netml-ns3-click: Modeling of Routers in Netml/ns3 by means of the Click Modular Router." In Eighth EAI International Conference on Simulation Tools and Techniques. ACM, 2015. http://dx.doi.org/10.4108/eai.24-8-2015.2260965.

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Rajankumar, Patel, Patel Nimisha, and Pariza Kamboj. "A comparative study and simulation of AODV MANET routing protocol in NS2 & NS3." In 2014 International Conference on Computing for Sustainable Global Development (INDIACom). IEEE, 2014. http://dx.doi.org/10.1109/indiacom.2014.6828091.

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Bhattacharya, Hindol, Samiran Chattopadhyay, and Matangini Chattopadhyay. "NS3 Based HDFS Data Placement Algorithm Evaluation Framework." In 2017 International Conference on Computer, Electrical & Communication Engineering (ICCECE). IEEE, 2017. http://dx.doi.org/10.1109/iccece.2017.8526204.

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Reports on the topic "Ns3"

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Ehrlich, Marcelo, John S. Parker, and Terence S. Dermody. Development of a Plasmid-Based Reverse Genetics System for the Bluetongue and Epizootic Hemorrhagic Disease Viruses to Allow a Comparative Characterization of the Function of the NS3 Viroporin in Viral Egress. United States Department of Agriculture, September 2013. http://dx.doi.org/10.32747/2013.7699840.bard.

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Project Title: "Development of a plasmid-based reverse genetics system for the Bluetongue and Epizootic Hemorrhagic Disease viruses to allow comparative characterization of the function of the NS3 viroporin in viral egress". Project details: No - IS-4192-09; Participants – Ehrlich M. (Tel Aviv University), Parker J.S. (Cornell University), DermodyT.S. (Vanderbilt University); Period - 2009-2013. Orbiviruses are insect-borne infectious agents of ruminants that cause diseases with considerable economical impact in Israel and the United States. The recent outbreaks of BTV in Europe and of Epizootic Hemorrhagic Disease Virus (EHDV) in Israel, underscore the need for: (i) a better comprehension of the infection process of orbiviruses, (ii) the identification of unique vs. common traits among different orbiviruses, (iii) the development of novel diagnosis and treatment techniques and approaches; all aimed at the achievement of more effective control and treatment measures. It is the context of these broad goals that the present project was carried out. To fulfill our long-term goal of identifying specific viral determinants of virulence, growth, and transmission of the orbiviruses, we proposed to: (i) develop reverse genetics systems for BTV and EHDV2-Ibaraki; and (ii) identify the molecular determinants of the NS3 nonstructural protein related to viroporin/viral egress activities. The first objective was pursued with a two-pronged approach: (i) development of a plasmid-based reverse genetics system for BTV-17, and (ii) development of an "in-vitro" transcription-based reverse genetics system for EHDV2-Ibaraki. Both approaches encountered technical problems that hampered their achievement. However, dissection of the possible causes of the failure to achieve viral spread of EHDV2-Ibaraki, following the transfection of in-vitro transcribed genomic segments of the virus, revealed a novel characteristic of EHDV2-Ibaraki infection: an uncharacteristically low fold increase in titer upon infection of different cell models. To address the function and regulation of NS3 we employed the following approaches: (i) development (together with Anima Cell Metrology) of a novel technique (based on the transfection of fluorescently-labeledtRNAs) that allows for the detection of the levels of synthesis of individual viral proteins (i.e. NS3) in single cells; (ii) development of a siRNA-mediated knockdown approach for the reduction in levels of expression of NS3 in EHDV2-Ibaraki infected cells; (iii) biochemical and microscopy-based analysis of the localization, levels and post-translational modifications of NS3 in infected cells. In addition, we identified the altered regulation and spatial compartmentalization of protein synthesis in cells infected with EHDV2-Ibaraki or the mammalian reovirus. In EHDV2-Ibaraki-infected cells such altered regulation in protein synthesis occurs in the context of a cell stress reponse that includes the induction of apoptosis, autophagy and activation of the stressrelated kinase c-Jun N-terminal Kinase (JNK). Interestingly, inhibition of such stress-related cellular processes diminishes the production of infectious virions, suggesting that EHDV usurps these responses for the benefit of efficient infection. Taken together, while the present project fell short of the generation of novel reverse genetics systems for orbiviruses, the development of novel experimental approaches and techniques, and their employment in the analysis of EHDV-infected cells, yielded novel insights in the interactions of orbiviruses with mammalian cells.
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Perk, Shimon, Maricarmen Garcia, Alexander Panshin, Caroline Banet-Noach, Irina Gissin, Mark W. Jackwood, and David Stallknecht. Avian Influenza Virus H9N2: Characterization and Control Strategies. United States Department of Agriculture, June 2007. http://dx.doi.org/10.32747/2007.7709882.bard.

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Control of Avian Influenza (AI) infection is a highly topical subject of major economicimportance for the worldwide poultry industry at the national level and for international trade.H9N2 viruses are endemic in poultry throughout Asia and the Middle East, causing major losses inproduction. Moreover, these viruses pose wider threats since they have been isolated from bothswine and humans. At the same time, study of the AI viruses affords an opportunity to explore anumber of problems of intriguing scientific interest. The overall goal of this project was to developa sound control strategy for avian influenza subtype H9N2 viruses (AI H9N2) in commercialpoultry in Israel. The one-year feasibility study focused on two main goals, namely: to study themolecular characteristics of AI H9N2 circulating during the last seven years in Israel and todevelop tools enabling differentiation between the immune response to vaccination and infectionwith H9N2.Genetic and phylogenetic characterization of 29 selected AI H9N2 isolates (2000-2006)was performed by complete sequencing of hemagglutinin (HA), neuraminidase (NA), and all sixinternal genes [nucleoprotein (NP), polymerase basic 1 (PB1), polymerase basic 2 (PB2),polymerase acid (PA), matrix (M), and nonstructural (NS) genes]; comparative phylogenetic andgenetic analyses of these sequences; and comparative genetic analyses of deduced amino acidsequences of the HA, NA, NS1, and NS2 proteins. The major conclusions of the molecularanalyses were: (1) Israeli isolates, together with other H9N2 viruses isolated in Middle Eastcountries, comprise a single regional sublineage related to the G1-lineage. In addition, Israeliisolates subdivided into three different subgroups. Genetic analysis of these viruses suggests thatthey underwent divergent evolution paths.
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Agrimson, Erick Paul, Gordon McIntosh, James Flaten, Kaye Smith, Bernhard Beck-Winchatz, Hank D. Voss, Donald Takehara, and Stacy A. Wenzel. NSF IUSE Workshop. Ames (Iowa): Iowa State University. Library. Digital Press, January 2015. http://dx.doi.org/10.31274/ahac.9774.

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Perk, Simon, Egbert Mundt, Alexander Panshin, Irit Davidson, Irina Shkoda, Ameera AlTori, and Maricarmen Garcia. Characterization and Control Strategies of Low Pathogenic Avian Influenza Virus H9N2. United States Department of Agriculture, November 2012. http://dx.doi.org/10.32747/2012.7697117.bard.

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The avian influenza virus, subtype H9N2 subtype, defined as having a low pathogenicity, causes extensive economical losses in commercial flocks, probably due to management and synergism with other pathogens. AIV H9N2 was first identified in Israel in the year 2000, and since then it became endemic and widespread in Israel. Control by vaccination of commercial flocks with an inactivated vaccine has been introduced since 2007. In face of the continuous H9N2 outbreaks, and the application of the vaccination policy, we aimed in the present study to provide a method of differentiating naturally infected from vaccinated animals (DIVA). The aim of the assay would be detect only antibodies created by a de-novo infection, since the inactivated vaccine virus is not reproducing, and might provide a simple tool for mass detection of novel infections of commercial flocks. To fulfill the overall aim, the project was designed to include four operational objectives: 1. Evaluation of the genetic evolution of AIV in Israel; 2. Assessment of the diagnostic value of an NS1 ELISA; 3. NS1 ELISA as evaluation criteria for measuring the efficacy of vaccination against H9N2 AIV; 4. Development of an AIV H9 subtype specific ELISA systems. Major conclusion and implications drawn from the project were: 1. A continuous genetic change occurred in the collection of H9N2 isolates, and new introductions were identified. It was shown thatthe differences between the HA proteins of viruses used for vaccine productionand local fieldisolatesincreasedin parallelwith the durationand intensity ofvaccine use, therefore, developing a differential assay for the vaccine and the wild type viruses was the project main aim. 2. To assess the diagnostic value of an NS1 ELISA we first performed experimental infection trials using representative viruses of all introductions, and used the sera and recombinant NS1 antigens of the same viruses in homologous and heterologous NS1 ELISA combination. The NS1 ELISA was evidently reactive in all combinations, and did not discriminate significantly between different groups. 3. However, several major drawbacks of the NS1 ELISA were recognized: a) The evaluation of the vaccination effect in challenged birds, showed that the level of the NS1 antibodies dropped due to the vaccination-dependent virus level drop; b) the applicability of the NS1-ELISA was verified on sera of commercial flocks and found to be unusable due to physico-chemical composition of the sera and the recombinant antigen, c) commercial sera showed non-reactivity that might be caused by many factors, including vaccination, uncertainty regarding the infection time, and possibly low antigen avidity, d) NS1 elevated antibody levels for less than 2 months in SPF chicks. Due to the above mentioned reasons we do not recommend the application of the DIVA NS1 ELISA assay for monitoring and differentiation AIV H9N2 naturally-infected from vaccinated commercial birds.
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Liu, Edgar, Malgorzata Lagisz, Evelyne de Leeuw, and Hyungmo Yang. Place-based Health Interventions in NSW - A rapid review of evidence. SPHERE HUE Collaboratory, November 2022. http://dx.doi.org/10.52708/pbhi-el.

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This report describes a rapid review exercise on the place-based intervention approaches to improving the health and wellbeing outcomes of residents in the Australian state of New South Wales (NSW). The aim of this exercise is to inform the Cancer Institute NSW on their future policy and program developments in cancer prevention and screening. Specifically, it seeks to answer the following research questions: 1. What place-based interventions for health promotion and risk prevention and screening currently exist in NSW? 2. How effective have these interventions been in achieving their stated objectives?
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Beck, James B. NSO News October 2013. Office of Scientific and Technical Information (OSTI), November 2013. http://dx.doi.org/10.2172/1104903.

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Beck, James B. NSO News September 2013. Office of Scientific and Technical Information (OSTI), November 2013. http://dx.doi.org/10.2172/1104904.

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Beck, James B. NSO News January 2014. Office of Scientific and Technical Information (OSTI), February 2014. http://dx.doi.org/10.2172/1119587.

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Beck, James B. NSO News - February 2014. Office of Scientific and Technical Information (OSTI), March 2014. http://dx.doi.org/10.2172/1122052.

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Sparks, Valerie, Frederick M. Helsel, Daniel A. Lucero, and Darielle Dexheimer. ARM/NSA Monthly Report. Office of Scientific and Technical Information (OSTI), November 2016. http://dx.doi.org/10.2172/1331868.

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