Academic literature on the topic 'Nuclear 28S rRNA gene'

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Journal articles on the topic "Nuclear 28S rRNA gene"

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Labidi, B., F. Broders, J. L. Meyer, and D. Hernandez-Verdun. "Distribution of rDNA and 28S, 18S, and 5S rRNA in micronuclei containing a single chromosome." Biochemistry and Cell Biology 68, no. 6 (1990): 957–64. http://dx.doi.org/10.1139/o90-141.

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Investigating genes and their transcription products in nuclear compartments corresponding to one mammalian chromosome, the ribosomal genes 18S–28S and 5S were localized in PtK1 micronucleated cells and rRNA was characterized in sorted micronuclei containing single identified chromosomes. In situ hybridization revealed the presence of 18S–28S rRNA genes in two micronuclei per cell and 5S rRNA genes in four micronuclei per cell. Flow cytometry histograms of isolated micronuclei stained with Hoechst 33342 exhibited five peaks (a–e) in which peaks b and c, respectively, corresponded to chromosome
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Meemongkolkiat, Thitipan, Atsalek Rattanawannee, and Chanpen Chanchao. "Genetic Diversity of Apis spp. in Thailand Inferred from 28S rRNA Nuclear and Cytochrome b Mitochondrial Gene Sequences." Psyche: A Journal of Entomology 2019 (February 20, 2019): 1–11. http://dx.doi.org/10.1155/2019/5823219.

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Knowledge of the genetic diversity of Apis spp. is important in order to provide a better understanding of breeding strategies that relate to the conservation of wild species and colony survival of farmed species. Here, honeybees of five Apis species were collected from 12 provinces throughout Thailand. After DNA extraction, 28S rRNA nuclear (710 bp) and cytochrome b (cytb) mitochondrial (520 bp) gene fragments were sequenced. Homologous sequences (nucleotide identity of over 95%) were obtained from GeneBank using the BLASTn algorithm, aligned, and analysed by maximum likelihood and Bayesian i
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BORGES, LUÍSA M. S., and LUCAS M. MERCKELBACH. "Lyrodus mersinensis sp. nov. (Bivalvia: Teredinidae) another cryptic species in the Lyrodus pedicellatus (Quatrefages, 1849) complex." Zootaxa 4442, no. 3 (2018): 441. http://dx.doi.org/10.11646/zootaxa.4442.3.6.

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New data from barcode index numbers (BINs) and 28S rRNA gene sequences confirm a cryptic species pair in Lyrodus pedicellatus from the eastern Mediterranean and European Atlantic coasts. Therefore, it is paramount to associate the new species to a scientific name for a reliable reference system of biological information. To this end, we describe Lyrodus mersinensis sp. nov., another cryptic species in the L. pedicellatus complex, and redescribe the `true´ L. pedicellatus. Both the description and redescription are based on molecular diagnostic characters obtained from sequences of the mitochon
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Hadjiolova, K. V., A. Normann, J. Cavaillé, et al. "Processing of truncated mouse or human rRNA transcribed from ribosomal minigenes transfected into mouse cells." Molecular and Cellular Biology 14, no. 6 (1994): 4044–56. http://dx.doi.org/10.1128/mcb.14.6.4044-4056.1994.

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The processing of pre-rRNA in eukaryotic cells involves a complex pattern of nucleolytic reactions taking place in preribosomes with the participation of several nonribosomal proteins and small nuclear RNAs. The mechanism of these reactions remains largely unknown, mainly because of the absence of faithful in vitro assays for most processing steps. We have developed a pre-rRNA processing system using the transient expression of ribosomal minigenes transfected into cultured mouse cells. Truncated mouse or human rRNA genes are faithfully transcribed under the control of mouse promoter and termin
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Hadjiolova, K. V., A. Normann, J. Cavaillé, et al. "Processing of truncated mouse or human rRNA transcribed from ribosomal minigenes transfected into mouse cells." Molecular and Cellular Biology 14, no. 6 (1994): 4044–56. http://dx.doi.org/10.1128/mcb.14.6.4044.

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The processing of pre-rRNA in eukaryotic cells involves a complex pattern of nucleolytic reactions taking place in preribosomes with the participation of several nonribosomal proteins and small nuclear RNAs. The mechanism of these reactions remains largely unknown, mainly because of the absence of faithful in vitro assays for most processing steps. We have developed a pre-rRNA processing system using the transient expression of ribosomal minigenes transfected into cultured mouse cells. Truncated mouse or human rRNA genes are faithfully transcribed under the control of mouse promoter and termin
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Xu, Haowen, Zhailin Chu, Jing Zhang, Meidong Jing, and Ling Huang. "Genetic Diversity and Population Structure of Acanthochiton rubrolineatus (Polyplacophora) Based on Mitochondrial and Nuclear Gene Markers." Diversity 12, no. 4 (2020): 159. http://dx.doi.org/10.3390/d12040159.

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Acanthochiton rubrolineatus (Cryptoplacidae, Neoloricata, Polyplacophora) has a narrow distribution range along the seacoasts of China, the Korean Peninsula and Japan. We collected 238 samples from eight localities along the Chinese coast, and analyzed the genetic diversity and population structure with COI, 16S-rRNA and 28S-rRNA gene sequences. All analyses based on combined sequences of COI and 16S-rRNA suggested that there was evident genetic differentiation between the northern populations (YT, WH, DL, QD, LYG) and southern populations (ZS, YH, XM) of A. rubrolineatus. The haplotype distri
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Noblezada, Mary Mar, Hiroomi Miyamoto, Wilfredo L. Campos, Fatimah MD Yusoff, and Shuhei Nishida. "Phylogeography of the planktonic shrimp Lucifer hanseni Nobili 1905 in the Indo-Malayan Archipelago." Journal of the Marine Biological Association of the United Kingdom 97, no. 1 (2016): 129–40. http://dx.doi.org/10.1017/s0025315416000163.

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Using partial sequences of two mitochondrial genes, cytochrome c oxidase subunit I (COI) and 12S ribosomal RNA (12S rRNA), and one nuclear gene, 28S ribosomal RNA (28S rRNA), we investigated population genetics of the holoplanktonic shrimp Lucifer hanseni Nobili, 1905 in the Indo-Malayan Archipelago (IMA), encompassing Andaman Sea, Malacca Strait, Gulf of Thailand, Borneo Island, Philippines (hereafter collectively referred to as the Thailand-Malaysia-Philippine area: TMP), Celebes Sea (CS), and the waters near islands in the Western Pacific (WP) including Palau, Papua New Guinea and Solomon I
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Kim, Sangjin, Željko Tomanović, Andjeljko Petrović, et al. "Toxares koreanus sp. nov. – a new Toxares species from South Korea (Hymenoptera, Braconidae, Aphidiinae)." Journal of Hymenoptera Research 92 (August 31, 2022): 185–98. http://dx.doi.org/10.3897/jhr.92.84146.

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The genus Toxares Haliday, 1840 is a small taxon of Aphidiinae, consisting four valid species in the world. One Toxares species is recorded as new to science from South Korea, in this study. Descriptions and illustrations of the new species, T. koreanussp. nov., are provided, together with their mitochondrial cytochrome c oxidase subunit I (COI) and D2 region of the nuclear gene for 28S rRNA (28S) sequences. The phylogenetic tree reconstructed using a combination of COI and 28S revealed the phylogenetic position of the genus Toxares within Aphidiinae.
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Kim, Sangjin, Željko Tomanović, Andjeljko Petrović, et al. "Toxares koreanus sp. nov. – a new Toxares species from South Korea (Hymenoptera, Braconidae, Aphidiinae)." Journal of Hymenoptera Research 92 (August 31, 2022): 185–98. https://doi.org/10.3897/jhr.92.84146.

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The genus Toxares Haliday, 1840 is a small taxon of Aphidiinae, consisting four valid species in the world. One Toxares species is recorded as new to science from South Korea, in this study. Descriptions and illustrations of the new species, T. koreanus sp. nov., are provided, together with their mitochondrial cytochrome c oxidase subunit I (COI) and D2 region of the nuclear gene for 28S rRNA (28S) sequences. The phylogenetic tree reconstructed using a combination of COI and 28S revealed the phylogenetic position of the genus Toxares within Aphidiinae.
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LIU, HUI, PING ZHAO, SHUJUAN LI, and WANZHI CAI. "Taxonomic status of Velinoides Matsumura (Hemiptera: Reduviidae: Harpactorinae) inferred from mitochondrial and nuclear genes." Zootaxa 2080, no. 1 (2009): 55–68. http://dx.doi.org/10.11646/zootaxa.2080.1.5.

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In this study, we used three mitochondrial genes (cyt b, COI, and 16S rRNA) and one nuclear gene (28S rRNA) to evaluate the current taxonomic status of the genus Velinoides erected by Matsumura. Phylogenetic analyses of genes using maximum parsimony, maximum likelihood, and minimum evolution resulted in different phylogenetic trees. However, the combined dataset analysis revealed better phylogenetic relationships. The constructed phylogenies appeared to be largely congruent with morphological studies. Results based on the molecular data strongly supported that the C. dilatatus, the type specie
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Dissertations / Theses on the topic "Nuclear 28S rRNA gene"

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Wakeman, Jane A. "Human ribosomal RNA : primary structure analysis of the 28S rRNA gene and preliminary studies on the distribution of pseudouridine residues in the 18S and 28S rRNA molecules." Thesis, University of Liverpool, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317264.

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Ljiljana, Šašić Zorić. "Молекуларни диверзитет Merodon aureus групе (Diptera: Syrphidae)". Phd thesis, Univerzitet u Novom Sadu, Prirodno-matematički fakultet u Novom Sadu, 2018. https://www.cris.uns.ac.rs/record.jsf?recordId=107246&source=NDLTD&language=en.

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Род <em>Merodon Meigen</em> (Diptera: Syrphidae) се одликује великм бројем врста које имају&nbsp; функцију у опрашивању&nbsp; биљака. У оквиру овог рода својом разноврсношћу издваја се <em>Merodon aureus</em>&nbsp; група&nbsp; врста коју поред фенотипски различитих одликује и присуство већег броја криптичних&nbsp; врста. Због морфолошке сличности криптичне врсте представљају изазов за таксономе, те су молекуларне методе од посебног значаја. У том светлу примарни циљ овог истраживања је био утврђивање молекуларног диверзитета групе и могућности његове примене у таксономији. Истраживање је било
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Li, Fei. "Nuclear genes that promote splicing of the chloroplast group-I 23S rRNA intron and an organelle intron database, FUGOID." 2002. http://wwwlib.umi.com/cr/utexas/fullcit?p3099478.

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Kwaśnik, Aleksandra. "DXO1 links nuclear gene expression and chloroplast function in Arabidopsis thaliana." Doctoral thesis, 2019. https://depotuw.ceon.pl/handle/item/3530.

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Białka z rodziny DXO/Rai1 są zaangażowane w kontrolę jakości końca 5' mRNA (5'QC), usuwanie niekanonicznego kapu NAD+ (deNADding) oraz dojrzewanie rybosomalnego RNA (rRNA) w komórkach drożdżowych. Poniższa praca opisuje aktywność biochemiczną i znaczenie fizjologiczne białka DXO1 z Arabidopsis thaliana. Analizy enzymatyczne wykazały, że DXO1 bardzo skutecznie usuwa kap NAD+ oraz posiada słabą aktywność egzorybonukleazy 5'-3'. Pozostałe właściwości biochemiczne charakterystyczne dla homologów DXO/Rai1 są zahamowane w roślinnym DXO1 na skutek obecności pojedynczej substytucji aminokwasu w obrębi
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Chuan-PinYang and 楊顓賓. "Characterization of MSP58 as a regulator of rRNA gene transcription and mapping of the nuclear/nucleolar localization signals." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/v5y465.

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碩士<br>國立成功大學<br>生物資訊與訊息傳遞研究所<br>102<br>The 58-kDa microspherule protein (MSP58; MCRS1) is a nucleolar protein controlling the cell proliferation, transformation and senescence, It had been found that there is a typical nuclear localization signal (NLS) and a bipartite nucleolar localization signal (NoLS) within MCRS2. Additionally, using the yeast two-hybrid system, we identified importin α1 as a MSP58-interacting protein. Therefore we tried to determine the presence of NLS and NoLS within the MSP58. Using site-specific mutagenesis and immunofluorescence analysis, we have identified two NLSs s
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Book chapters on the topic "Nuclear 28S rRNA gene"

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Schächner, Christopher, Philipp E. Merkl, Michael Pilsl, et al. "Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes." In Ribosome Biogenesis. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2501-9_2.

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AbstractIn growing eukaryotic cells, nuclear ribosomal (r)RNA synthesis by RNA polymerase (RNAP) I accounts for the vast majority of cellular transcription. This high output is achieved by the presence of multiple copies of rRNA genes in eukaryotic genomes transcribed at a high rate. In contrast to most of the other transcribed genomic loci, actively transcribed rRNA genes are largely devoid of nucleosomes adapting a characteristic “open” chromatin state, whereas a significant fraction of rRNA genes resides in a transcriptionally inactive nucleosomal “closed” chromatin state. Here, we review o
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Christen, R., and J. C. Braconnot. "Molecular phylogeny of tunicates. A preliminary study using 28S ribosomal RNA partial sequences: implications in terms of evolution and ecology." In The Biology of Pelagic Tunicates. Oxford University PressOxford, 1998. http://dx.doi.org/10.1093/oso/9780198540243.003.0016.

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Abstract Phylogenetic information can now be derived from the comparison of nucleic acid sequences that are strictly homologous in different taxa. Among available gene sequences, ribosomal RNAs (rRNA) have provided some of the most valuable phylogenetic information. Three rRNA molecules have been generally used, namely the 5S rRNA, 16-18S rRNA and 26-28S rRNA. Phylogenies derived from the 5S molecule are now thought to be less reliable because of an inappropriate rate of evolution (Halanych, 1991). In contrast, both the 16-18S rRNA and the 23-28S rRNA are now widely used (Sogin et al., 1986; B
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"Molecular Markers for Phylogenetic Studies and Germplasm Conservation." In Advances in Environmental Engineering and Green Technologies. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-4312-2.ch005.

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Application of molecular markers in phylogenetic studies has become increasingly important in recent times. Availability of fast DNA sequencing techniques and robust statistical analysis methods provided new momentum to this field. Different nuclear encoded genes (16S rRNA, 5S rRNA, 28S rRNA), mitochondrial encoded genes (cytochrome oxidase, mitochondrial 12S, cytochrome b, control region), and few chloroplast encoded genes (rbcL, matK, rpi16) have been used as molecular markers. This method allows researchers to obtain new evidence concerning their phylogeny and biodiversity. Measurement of g
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Neara, Thomas J., and Masaki Miyab. "Ray-finned fishes (Actinopterygii)." In The Timetree of Life. Oxford University PressOxford, 2009. http://dx.doi.org/10.1093/oso/9780199535033.003.0042.

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Abstract Actinopterygii, or ray-Anned Ashes, are one of the two major lineages of osteichthyan vertebrates, the other being Sarcopterygii (1). 7ere are more than 26,890 species of actinopterygian Ashes and the group has diversiAed into a wide range of marine and freshwater habitats (2). Typically Ave major clades are recognized in Actinopterygii: Polypteriformes (bichirs), Acipenseriformes (sturgeons and paddleAshes), Lepisosteiformes (gars), Amiiformes (BowAn), and Teleostei. In this account, we review the evidence presented for the monophyly of Actinopterygii, the phylogenetic hypotheses of
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Lucchesi, John C. "The nucleolus." In Epigenetics, Nuclear Organization & Gene Function. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198831204.003.0012.

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The nucleolus forms at nucleolus organizer regions (NORs) that consist of clusters of repeated rRNA genes. Transcription of the rRNA genes and processing of the transcripts yields the three types of RNAs necessary for the biogenesis of ribosomes. Only subsets of the rRNA genes present in cells are transcribed. The linker histone H1 plays a specific role in the repression of inactive rRNA genes and in many of the other functions of the nucleolus. One of these functions is gene silencing—the nucleolus is surrounded by a zone of heterochromatin consisting of silenced rRNA gene arrays, DNA repeats
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Lucchesi, John C. "Nuclear bodies." In Epigenetics, Nuclear Organization & Gene Function. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198831204.003.0013.

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The nucleus is subdivided into a number of compartments that are not enclosed by membranes and whose main functions are transcriptional regulation and RNA processing. Many of the same proteins are found in different compartments, highlighting the dynamic exchange of components. The perinuclear compartment (PNC) is a hallmark of a number of different cancers. Cajal bodies (CBs) are sites of assembly of small nuclear ribonucleic particles (snRNPs) that function in messenger RNA (mRNA) or ribosomal RNA (rRNA) processing and in the biogenesis of telomerase. Nuclear speckles contain pre-mRNA splici
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Lucchesi, John C. "Regulation of domains and whole chromosomes." In Epigenetics, Nuclear Organization & Gene Function. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198831204.003.0009.

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Clusters of genes that encode similar products, such as the β‎-globin, the ribosomal RNA (rRNA) and the histone genes, are regulated in a coordinated fashion. An extreme case of coordinate regulation—dosage compensation—involves the genes present on the sex chromosomes. In Drosophila males, a complex (MSL) associates with the X chromosome where it enhances the activity of most X-linked genes. In Caenorhabditis, a complex (DCC) decreases the level of transcription of both X chromosomes in the XX hermaphrodite. In mammals, dosage compensation is achieved by the inactivation, early during develop
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Lucchesi, John C. "The role of non-coding RNAs." In Epigenetics, Nuclear Organization & Gene Function. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198831204.003.0006.

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Most of the genome is transcribed into non-coding transcripts that far exceed in number the transcripts of protein-coding genes. These RNAs are subdivided into different classes. Long non-coding RNAs (lncRNAs) are at least 200 nucleotides in length and are transcribed from promoter, coding, intergenic or enhancer regions (eRNAs). These RNAs repress or enhance the transcription of target genes by facilitating the interaction between promoters and enhancers or by interacting with transcription factors and targeting histone-modifying enzymes. Short non-coding RNAs include a diverse group of funct
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SWANSON, MAURICE S., and JOHN P. ARIS. "Posttranscriptional Control: Nuclear RNA Processing." In Inborn Errors Of Development. Oxford University PressNew York, NY, 2008. http://dx.doi.org/10.1093/oso/9780195306910.003.0125.

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Abstract Normal development of human tissues requires the activation of specific genes during various embryonic stages so that stage-specific protein isoforms and other gene products appear in their proper developmental windows. While transcriptional initiation starts the gene expression cascade, nascent transcripts must be modified and the mature RNA products correctly localized within the cell. Most of the nuclear processing machinery is devoted toward producing the major RNAs required for the translational apparatus (Fig. 125–1). Pre-messenger RNAs (pre-mRNAs) are transcribed by RNA polymer
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O.M. Al-Dahmoshi, Hussein, and Hayder J. Al-Nayili. "Mitochondrial 16S rRNA Gene-Dependent Blood Typing as a Forensic Tool." In Forensic Analysis [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.98248.

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Mitochondrial DNA is an important tool for human identification and is used to differentiate between human and animal blood at the crime scene, because in extreme conditions nuclear DNA is severely destroyed while Mitochondrial DNA contains multiple copies (200–2000) per cell and resists harsh and more stable conditions. Seventy-two blood samples were collected from humans (Homo sapiens), sheep (Ovis aries), goats (Capra hircus), and cows (Bos taurus) (18 blood samples for each). All blood samples were withdrawn by a technician and 5 ml were aspirated using an aseptic technique and transferred
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Conference papers on the topic "Nuclear 28S rRNA gene"

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Grigoryan, Alexander, Gideon M. Wolfaardt, Simcha Stroes-Gascoyne, et al. "Baseline Distribution and Diversity of MIC-Relevant Microorganisms in Compacted Bentonites after Incubation for 1 Year." In CORROSION 2020. NACE International, 2020. https://doi.org/10.5006/c2020-15020.

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Abstract The Materials Corrosion Test (MaCoTe) project at the Grimsel Test Site (Switzerland) is being used to provide experimental data for modeling the effect of the bentonite buffer on microbially-influenced corrosion (MIC) in deep geological repositories for spent nuclear fuel. Using culture-based and molecular culture-independent methods, we evaluated bacterial characteristics in bentonites compacted to bulk densities of 1.25 g/cm3 and 1.5 g/cm3 after one-year in-situ incubation in MaCoTe borehole modules. Abundance of aerobic and anaerobic microorganisms in the uncompacted MX-80 bentonit
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Peck, Kayla, Robert Stedtfeld, Jordan RoseFigura, et al. "Abstract 1484: Microbial sequencing using a single-pool target enrichment of multiple variable regions of the 16S rRNA gene, the nuclear ribosomal internal transcribed spacer (ITS) region, and antimicrobial resistance genes." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-1484.

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Peck, Kayla, Robert Stedtfeld, Jordan RoseFigura, et al. "Abstract 1484: Microbial sequencing using a single-pool target enrichment of multiple variable regions of the 16S rRNA gene, the nuclear ribosomal internal transcribed spacer (ITS) region, and antimicrobial resistance genes." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-1484.

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Reports on the topic "Nuclear 28S rRNA gene"

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วิเวกแว่ว, อัมพร, та วิเชฏฐ์ คนซื่อ. การแปรผันทางพันธุกรรมและการประเมินความเป็นไปได้ในการผสมข้ามสายพันธุ์ระหว่างอึ่งน้ำเต้า (Microhyla fissipes) อึ่งข้างดำ (M. heymonsi) และอึ่งลายเลอะ (M. butleri) ในพื้นที่สวนสัตว์เปิดเขาเขียว จังหวัดชลบุรี โดยใช้นิวเคลียร์ยีนเป็นดีเอ็นเอเครื่องหมายในการตรวจ : รายงานผลการดำเนินงาน. จุฬาลงกรณ์มหาวิทยาลัย, 2015. https://doi.org/10.58837/chula.res.2015.67.

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อึ่งน้ำเต้า (Microhyla fissipes) อึ่งข้างดำ (M. heymonsi) และอึ่งลายเลอะ (M. butleri) จัดเป็นสัตว์ สะเทินน้ำสะเทินบกที่อยู่ในสกุลเดียวกัน มีขนาดตัวใกล้เคียงกันและมีการกระจายอยู่ในทุกภาคของประเทศไทย การศึกษาครั้งนี้สนใจตรวจสอบความหลากหลายทางพันธุกรรมของยีน 28S rRNA และประเมินความเป็นไปได้ใน การเกิดการผสมข้ามสายพันธุ์ระหว่างอึ่งทั้งสามชนิดในพื้นที่สวนสัตว์เปิดเขาเขียว จังหวัดชลบุรี โดยนำอึ่งน้ำเต้า จำนวน 19 ตัว อึ่งข้างดำจำนวน 10 ตัว และอึ่งลายเลอะจำนวน 6 ตัว มาสกัดดีเอ็นเอ เพิ่มปริมาณยีน 28S rRNA ในนิวเคลียร์ดีเอ็นเอโดยใช้เทคนิคพีซีอาร์และหาลำดับนิวคลีโอไทด์ ผลการศึกษาพบว่าผลิตภัณฑ์พีซีอาร์ของอ
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