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Journal articles on the topic 'Nuclear 28S rRNA gene'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Labidi, B., F. Broders, J. L. Meyer, and D. Hernandez-Verdun. "Distribution of rDNA and 28S, 18S, and 5S rRNA in micronuclei containing a single chromosome." Biochemistry and Cell Biology 68, no. 6 (1990): 957–64. http://dx.doi.org/10.1139/o90-141.

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Investigating genes and their transcription products in nuclear compartments corresponding to one mammalian chromosome, the ribosomal genes 18S–28S and 5S were localized in PtK1 micronucleated cells and rRNA was characterized in sorted micronuclei containing single identified chromosomes. In situ hybridization revealed the presence of 18S–28S rRNA genes in two micronuclei per cell and 5S rRNA genes in four micronuclei per cell. Flow cytometry histograms of isolated micronuclei stained with Hoechst 33342 exhibited five peaks (a–e) in which peaks b and c, respectively, corresponded to chromosome

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2

Meemongkolkiat, Thitipan, Atsalek Rattanawannee, and Chanpen Chanchao. "Genetic Diversity of Apis spp. in Thailand Inferred from 28S rRNA Nuclear and Cytochrome b Mitochondrial Gene Sequences." Psyche: A Journal of Entomology 2019 (February 20, 2019): 1–11. http://dx.doi.org/10.1155/2019/5823219.

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Knowledge of the genetic diversity of Apis spp. is important in order to provide a better understanding of breeding strategies that relate to the conservation of wild species and colony survival of farmed species. Here, honeybees of five Apis species were collected from 12 provinces throughout Thailand. After DNA extraction, 28S rRNA nuclear (710 bp) and cytochrome b (cytb) mitochondrial (520 bp) gene fragments were sequenced. Homologous sequences (nucleotide identity of over 95%) were obtained from GeneBank using the BLASTn algorithm, aligned, and analysed by maximum likelihood and Bayesian i

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BORGES, LUÍSA M. S., and LUCAS M. MERCKELBACH. "Lyrodus mersinensis sp. nov. (Bivalvia: Teredinidae) another cryptic species in the Lyrodus pedicellatus (Quatrefages, 1849) complex." Zootaxa 4442, no. 3 (2018): 441. http://dx.doi.org/10.11646/zootaxa.4442.3.6.

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New data from barcode index numbers (BINs) and 28S rRNA gene sequences confirm a cryptic species pair in Lyrodus pedicellatus from the eastern Mediterranean and European Atlantic coasts. Therefore, it is paramount to associate the new species to a scientific name for a reliable reference system of biological information. To this end, we describe Lyrodus mersinensis sp. nov., another cryptic species in the L. pedicellatus complex, and redescribe the `true´ L. pedicellatus. Both the description and redescription are based on molecular diagnostic characters obtained from sequences of the mitochon

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4

Hadjiolova, K. V., A. Normann, J. Cavaillé, et al. "Processing of truncated mouse or human rRNA transcribed from ribosomal minigenes transfected into mouse cells." Molecular and Cellular Biology 14, no. 6 (1994): 4044–56. http://dx.doi.org/10.1128/mcb.14.6.4044-4056.1994.

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The processing of pre-rRNA in eukaryotic cells involves a complex pattern of nucleolytic reactions taking place in preribosomes with the participation of several nonribosomal proteins and small nuclear RNAs. The mechanism of these reactions remains largely unknown, mainly because of the absence of faithful in vitro assays for most processing steps. We have developed a pre-rRNA processing system using the transient expression of ribosomal minigenes transfected into cultured mouse cells. Truncated mouse or human rRNA genes are faithfully transcribed under the control of mouse promoter and termin

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Hadjiolova, K. V., A. Normann, J. Cavaillé, et al. "Processing of truncated mouse or human rRNA transcribed from ribosomal minigenes transfected into mouse cells." Molecular and Cellular Biology 14, no. 6 (1994): 4044–56. http://dx.doi.org/10.1128/mcb.14.6.4044.

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The processing of pre-rRNA in eukaryotic cells involves a complex pattern of nucleolytic reactions taking place in preribosomes with the participation of several nonribosomal proteins and small nuclear RNAs. The mechanism of these reactions remains largely unknown, mainly because of the absence of faithful in vitro assays for most processing steps. We have developed a pre-rRNA processing system using the transient expression of ribosomal minigenes transfected into cultured mouse cells. Truncated mouse or human rRNA genes are faithfully transcribed under the control of mouse promoter and termin

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6

Xu, Haowen, Zhailin Chu, Jing Zhang, Meidong Jing, and Ling Huang. "Genetic Diversity and Population Structure of Acanthochiton rubrolineatus (Polyplacophora) Based on Mitochondrial and Nuclear Gene Markers." Diversity 12, no. 4 (2020): 159. http://dx.doi.org/10.3390/d12040159.

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Acanthochiton rubrolineatus (Cryptoplacidae, Neoloricata, Polyplacophora) has a narrow distribution range along the seacoasts of China, the Korean Peninsula and Japan. We collected 238 samples from eight localities along the Chinese coast, and analyzed the genetic diversity and population structure with COI, 16S-rRNA and 28S-rRNA gene sequences. All analyses based on combined sequences of COI and 16S-rRNA suggested that there was evident genetic differentiation between the northern populations (YT, WH, DL, QD, LYG) and southern populations (ZS, YH, XM) of A. rubrolineatus. The haplotype distri

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7

Noblezada, Mary Mar, Hiroomi Miyamoto, Wilfredo L. Campos, Fatimah MD Yusoff, and Shuhei Nishida. "Phylogeography of the planktonic shrimp Lucifer hanseni Nobili 1905 in the Indo-Malayan Archipelago." Journal of the Marine Biological Association of the United Kingdom 97, no. 1 (2016): 129–40. http://dx.doi.org/10.1017/s0025315416000163.

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Using partial sequences of two mitochondrial genes, cytochrome c oxidase subunit I (COI) and 12S ribosomal RNA (12S rRNA), and one nuclear gene, 28S ribosomal RNA (28S rRNA), we investigated population genetics of the holoplanktonic shrimp Lucifer hanseni Nobili, 1905 in the Indo-Malayan Archipelago (IMA), encompassing Andaman Sea, Malacca Strait, Gulf of Thailand, Borneo Island, Philippines (hereafter collectively referred to as the Thailand-Malaysia-Philippine area: TMP), Celebes Sea (CS), and the waters near islands in the Western Pacific (WP) including Palau, Papua New Guinea and Solomon I

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8

Kim, Sangjin, Željko Tomanović, Andjeljko Petrović, et al. "Toxares koreanus sp. nov. – a new Toxares species from South Korea (Hymenoptera, Braconidae, Aphidiinae)." Journal of Hymenoptera Research 92 (August 31, 2022): 185–98. http://dx.doi.org/10.3897/jhr.92.84146.

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The genus Toxares Haliday, 1840 is a small taxon of Aphidiinae, consisting four valid species in the world. One Toxares species is recorded as new to science from South Korea, in this study. Descriptions and illustrations of the new species, T. koreanussp. nov., are provided, together with their mitochondrial cytochrome c oxidase subunit I (COI) and D2 region of the nuclear gene for 28S rRNA (28S) sequences. The phylogenetic tree reconstructed using a combination of COI and 28S revealed the phylogenetic position of the genus Toxares within Aphidiinae.

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Kim, Sangjin, Željko Tomanović, Andjeljko Petrović, et al. "Toxares koreanus sp. nov. – a new Toxares species from South Korea (Hymenoptera, Braconidae, Aphidiinae)." Journal of Hymenoptera Research 92 (August 31, 2022): 185–98. https://doi.org/10.3897/jhr.92.84146.

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The genus Toxares Haliday, 1840 is a small taxon of Aphidiinae, consisting four valid species in the world. One Toxares species is recorded as new to science from South Korea, in this study. Descriptions and illustrations of the new species, T. koreanus sp. nov., are provided, together with their mitochondrial cytochrome c oxidase subunit I (COI) and D2 region of the nuclear gene for 28S rRNA (28S) sequences. The phylogenetic tree reconstructed using a combination of COI and 28S revealed the phylogenetic position of the genus Toxares within Aphidiinae.

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LIU, HUI, PING ZHAO, SHUJUAN LI, and WANZHI CAI. "Taxonomic status of Velinoides Matsumura (Hemiptera: Reduviidae: Harpactorinae) inferred from mitochondrial and nuclear genes." Zootaxa 2080, no. 1 (2009): 55–68. http://dx.doi.org/10.11646/zootaxa.2080.1.5.

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In this study, we used three mitochondrial genes (cyt b, COI, and 16S rRNA) and one nuclear gene (28S rRNA) to evaluate the current taxonomic status of the genus Velinoides erected by Matsumura. Phylogenetic analyses of genes using maximum parsimony, maximum likelihood, and minimum evolution resulted in different phylogenetic trees. However, the combined dataset analysis revealed better phylogenetic relationships. The constructed phylogenies appeared to be largely congruent with morphological studies. Results based on the molecular data strongly supported that the C. dilatatus, the type specie

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Aitken, Stuart, and Colin A. Semple. "The circadian dynamics of small nucleolar RNA in the mouse liver." Journal of The Royal Society Interface 14, no. 130 (2017): 20170034. http://dx.doi.org/10.1098/rsif.2017.0034.

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The circadian regulation of gene expression allows plants and animals to anticipate predictable environmental changes. While the influence of the circadian clock has recently been shown to extend to ribosome biogenesis, the dynamics and regulation of the many small nucleolar RNA that are required in pre-ribosomal RNA folding and modification are unknown. Using a novel computational method, we show that 18S and 28S pre-rRNA are subject to circadian regulation in a nuclear RNA sequencing time course. A population of snoRNA with circadian expression is identified that is functionally associated w

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Pérez-Ponce de León, G., and D. I. Hernández-Mena. "Testing the higher-level phylogenetic classification of Digenea (Platyhelminthes, Trematoda) based on nuclear rDNA sequences before entering the age of the ‘next-generation’ Tree of Life." Journal of Helminthology 93, no. 3 (2019): 260–76. http://dx.doi.org/10.1017/s0022149x19000191.

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AbstractDigenea Carus, 1863 represent a highly diverse group of parasitic platyhelminths that infect all major vertebrate groups as definitive hosts. Morphology is the cornerstone of digenean systematics, but molecular markers have been instrumental in searching for a stable classification system of the subclass and in establishing more accurate species limits. The first comprehensive molecular phylogenetic tree of Digenea published in 2003 used two nuclear rRNA genes (ssrDNA = 18S rDNA and lsrDNA = 28S rDNA) and was based on 163 taxa representing 77 nominal families, resulting in a widely acc

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13

Kartavtsev, Yu Ph, S. N. Sharina, A. Yu Chichvarkhin, et al. "Genetic Divergence of Mussels (Mollusca, Mytilidae) Based on the 28S rRNA, 18S rRNA, and H3 Nuclear Gene Sequences." Russian Journal of Genetics 54, no. 6 (2018): 652–69. http://dx.doi.org/10.1134/s1022795418060078.

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14

Matumba, Tshifhiwa G., Jody Oliver, Nigel P. Barker, Christopher D. McQuaid, and Peter R. Teske. "Intraspecific mitochondrial gene variation can be as low as that of nuclear rRNA." F1000Research 9 (May 7, 2020): 339. http://dx.doi.org/10.12688/f1000research.23635.1.

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Background: Mitochondrial DNA (mtDNA) has long been used to date historical demographic events. The idea that it is useful for molecular dating rests on the premise that its evolution is neutral. Even though this idea has long been challenged, the evidence against clock-like evolution of mtDNA is often ignored. Here, we present a particularly clear and simple example to illustrate the implications of violations of the assumption of selective neutrality. Methods: DNA sequences were generated for the mtDNA COI gene and the nuclear 28S rRNA of two closely related rocky shore snails, and species-l

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Matumba, Tshifhiwa G., Jody Oliver, Nigel P. Barker, Christopher D. McQuaid, and Peter R. Teske. "Intraspecific mitochondrial gene variation can be as low as that of nuclear rRNA." F1000Research 9 (August 28, 2020): 339. http://dx.doi.org/10.12688/f1000research.23635.2.

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Background: Mitochondrial DNA (mtDNA) has long been used to date historical demographic events. The idea that it is useful for molecular dating rests on the premise that its evolution is neutral. Even though this idea has long been challenged, the evidence against clock-like evolution of mtDNA is often ignored. Here, we present a particularly clear and simple example to illustrate the implications of violations of the assumption of selective neutrality. Methods: DNA sequences were generated for the mtDNA COI gene and the nuclear 28S rRNA of two closely related rocky shore snails, and species-l

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16

Hao, Ke-Xin, Parisa Lotfollahi, and Xiao-Feng Xue. "Three New Eriophyid Mite Species from China (Acari: Eriophyidae)." Insects 14, no. 2 (2023): 159. http://dx.doi.org/10.3390/insects14020159.

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Eriophyid mites (Eriophyidae) are strictly phytophagous and are concentrated in Europe, Eastern Asia, Southeast Asia, Western and Eastern North America, Southern India, and New Zealand. South and southwest China are hot spots for eriophyid mite species diversity and endemism. In this study, we describe two new species, Scolotosus ehretussp. nov. on Ehretia acuminata (Boraginaceae) and Neotegonotus ulmchangussp. nov. on Ulmus changii (Ulmaceae), from south and southwest China (the Oriental Region), and one new eriophyid mite, Leipothrix ventricosissp. nov. on Hosta ventricosa (Asparagaceae), fr

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17

MENGUAL, XIMO, and F. CHRISTIAN THOMPSON. "A taxonomic review of the Palpada ruficeps species group, with the description of a new flower fly from Colombia (Diptera: Syrphidae)." Zootaxa 1741, no. 1 (2008): 31. http://dx.doi.org/10.11646/zootaxa.1741.1.3.

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A new species of flower flies, Palpada prietorum Mengual spec. nov. (Diptera: Syrphidae: Eristalinae) is described from Cali, Colombia. The new species belongs to the ruficeps subgroup in the vinetorum species group. An identification key is provided for the species of the ruficeps subgroup, as well as a comparison of morphological diagnostic characters to separate these taxa. Fragments of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the D2-3 region of the nuclear 28S rRNA gene and the nuclear internal transcribed spacer (ITS2) are supplied for two species, Palpada prietorum sp

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18

Carr, Martin, Peter Mayhew, and J. Peter Young. "Phylogeny of bethylid wasps (Hymenoptera: Bethylidae) inferred from 28S and 16S rRNA genes." Insect Systematics & Evolution 41, no. 1 (2010): 55–73. http://dx.doi.org/10.1163/187631210x486995.

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AbstractWe conduct the first molecular studies of the higher level phylogeny of bethylid wasps (Hymenoptera: Bethylidae). Sequences of the 500-bp D2/D3 expansion regions of the nuclear 28S rRNA gene are obtained for 33 bethylid species in 18 genera covering all four commonly recognized subfamilies, and an additional 13 outgroup species. A 450-bp sequence of the mitochondrial 16S rRNA gene is obtained for a smaller subset of those species. Strong support is found for the monophyly of Bethylidae, and for Bethylinae as the sister to the other bethylid subfamilies. Pristocerinae are supported as m

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Zamoroka, A. "Іs clytini monophyletic? The evidence from five-gene phylogenetic analysis". Proceedings of the State Natural History Museum, № 37 (1 січня 2022): 191–214. http://dx.doi.org/10.36885/nzdpm.2021.37.191-214.

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Tribe Clytini (Coleoptera: Cerambycidae) is one of the largest within the long horn beetles, having over 1500 species. Until now, the tribe was considered monophyletic, despite the fact that it combines several different morphological groups. Morphological data alone could not shed enough light on the taxonomy and phylogeny of Clytini. The data for the last decade on molecular phylogenetics have challenged the Clytini monophyletic hypothesis. In this study, I conducted a comprehensive phylogenetic analysis of Clytini based on the three mitochondrial genes 12S rRNA 16S rRNA COI and two nuclear

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20

Sun, Xiaoyan, and Jinhui Cheng. "Phylogenetic Signal Dissection of Heterogeneous 28S and 16S rRNA Genes in Spinicaudata (Branchiopoda, Diplostraca)." Genes 12, no. 11 (2021): 1705. http://dx.doi.org/10.3390/genes12111705.

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It is still a challenge to reconstruct the deep phylogenetic relationships within spinicaudatans, and there are several different competing hypotheses regarding the interrelationships among Eocyzicidae, Cyzicidae s. s., Leptestheriidae, and Limnadiidae of the Suborder Spinicaudata. In order to explore the source of the inconsistencies, we focus on the sequence variation and the structure model of two rRNA genes based on extensive taxa sampling. The comparative sequence analysis revealed heterogeneity across species and the existence of conserved motifs in all spinicaudatan species. The level o

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Wyngaard, G. A., I. A. McLaren, M. M. White, and J. M. Sévigny. "Unusually high numbers of ribosomal RNA genes in copepods (Arthropoda: Crustacea) and their relationship to genome size." Genome 38, no. 1 (1995): 97–104. http://dx.doi.org/10.1139/g95-012.

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We report on copy numbers of 18S ribosomal RNA genes in three species of copepods (Crustacea: Copepoda), two of which possess an unusual arrangement in which 5S genes are included within the 18S–5.8S–28S repeat unit. Slot blots of genomic and standard DNA were hybridized with an 18S rRNA gene probe constructed from one of the marine species and hybridization was quantified using chemiluminescence. Diploid 18S rRNA gene copy numbers are estimated as ca. 15 300 and 33 500 in the marine species Calanus finmarchicus (13.0 pg DNA in 2C adult nuclei) and C. glacialis (24.2 pg DNA), respectively, and

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Patwa, Nisha, Christopher Ranger, Maximilian Lehenberger, Peter Biedermann, and Michael Reding. "Stability of Nuclear and Mitochondrial Reference Genes in Selected Tissues of the Ambrosia Beetle Xylosandrus germanus." Insects 12, no. 12 (2021): 1125. http://dx.doi.org/10.3390/insects12121125.

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The fungus-farming ambrosia beetle Xylosandrus germanus (Blandford) uses a pouch-like structure (i.e., mycangium) to transport spores of its nutritional fungal mutualist. Our current study sought to identify reference genes necessary for future transcriptome analyses aimed at characterizing gene expression within the mycangium. Complementary DNA was synthesized using selected tissue types from laboratory-reared and field-collected X. germanus consisting of the whole body, head + thorax, deflated or inflated mycangium + scutellum, inflated mycangium, and thorax + abdomen. Quantitative reverse-t

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Xu, Biyang, Lu Qi, Lingfeng Kong, and Qi Li. "Description of Alvania wangi Xu, Qi & Kong, sp. nov. (Mollusca, Gastropoda, Littorinimorpha, Rissoidae) from the East China Sea." ZooKeys 1110 (July 6, 2022): 201–17. https://doi.org/10.3897/zookeys.1110.82173.

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Alvania wangi Xu, Qi & Kong, sp. nov. (Mollusca, Gastropoda, Littorinimorpha, Rissoidae) was discovered within the intertidal zone in the Nanji Islands and Zhoushan Islands, Zhejiang Province, China. It has a radula characteristic of Alvania Risso, 1826, a protoconch sculptured with micro pits and lamellae between spiral lirae, and a teleoconch with growth lines and subobsolete cords. Specimens were examined using an integrative taxonomic approach incorporating morphological observations and phylogenetic analyses of concatenated mitochondrial 16S rRNA and nuclear 28S rRNA gene sequences. T

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Zhu, Chengming, Qi Yan, Chenchun Weng, et al. "Erroneous ribosomal RNAs promote the generation of antisense ribosomal siRNA." Proceedings of the National Academy of Sciences 115, no. 40 (2018): 10082–87. http://dx.doi.org/10.1073/pnas.1800974115.

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Ribosome biogenesis is a multistep process, during which mistakes can occur at any step of pre-rRNA processing, modification, and ribosome assembly. Misprocessed rRNAs are usually detected and degraded by surveillance machineries. Recently, we identified a class of antisense ribosomal siRNAs (risiRNAs) that down-regulate pre-rRNAs through the nuclear RNAi pathway. To further understand the biological roles of risiRNAs, we conducted both forward and reverse genetic screens to search for more suppressor of siRNA (susi) mutants. We isolated a number of genes that are broadly conserved from yeast

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Wongsa, Kanyanat, Ekgachai Jeratthitikul, Pisit Poolprasert, Orawan Duangphakdee, and Atsalek Rattanawannee. "Genetic structure of the commercial stingless bee Heterotrigona itama (Apidae: Meliponini) in Thailand." PLOS ONE 19, no. 12 (2024): e0312386. https://doi.org/10.1371/journal.pone.0312386.

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Stingless beekeeping, also known as meliponiculture, has gained increasing popularity in many tropical and subtropical countries for its use in commercial pollination and high-value honey and propolis production. However, this rising interest in stingless beekeeping has led to significant geographical displacements of bee colonies by beekeepers, occasionally surpassing their native ranges. Consequently, this affects local bee populations by disrupting gene flow across unnaturally large geographic scales. For Heterotrigona itama, one of the most common stingless bee species in Southeast Asian c

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Enghoff, Henrik, Gitte Petersen, and Ole Seberg. "The aberrant millipede genus Pteridoiulus and its position in a revised molecular phylogeny of the family Julidae (Diplopoda : Julida)." Invertebrate Systematics 27, no. 5 (2013): 515. http://dx.doi.org/10.1071/is13016.

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A phylogenetic analysis of 62 species (32 genera) of the Palaearctic millipede family Julidae, including the aberrant alpine genus Pteridoiulus Verhoeff, 1913, was made based on partial sequences of the mitochondrial 16S rRNA (16S) gene and the nuclear 28S rRNA (28S) gene, respectively. The two datasets (16S rDNA and 28S rDNA) were analysed in combination but treated as different partitions using direct optimisation as implemented in POY. The 16S rDNA and the 28S rDNA sequences vary from 410 to 449 bp and from 467 to 525 bp in length, respectively. All searches were performed under six differe

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Ni, Gang, Qi Li, Lingfeng Kong, and Hong Yu. "The Clam Cyclina sinensis (Gmelin) Phylogeography Study with 28S rRNA Gene and Potential of Nuclear rRNA Genes in Genetic Assessments of Molluscs." Journal of Ocean University of China 21, no. 2 (2022): 395–99. http://dx.doi.org/10.1007/s11802-022-4845-1.

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Presswell, B., S. Evans, R. Poulin, and F. Jorge. "Morphological and molecular characterization of Mermisnigrescens Dujardin, (Nematoda: Mermithidae) parasitizing the introduced European earwig (Dermaptera: Forficulidae) in New Zealand." Journal of Helminthology 89, no. 3 (2014): 267–76. http://dx.doi.org/10.1017/s0022149x14000017.

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AbstractParasitic nematodes of the family Mermithidae were found to be infecting the introduced European earwig Forficula auricularia (Dermaptera: Forficulidae) in Dunedin, South Island, New Zealand. Adult females were later collected from various garden plants while depositing eggs. These mermithid specimens were identified morphologically as Mermis nigrescens Dujardin, 1842. A genetic distance of 0.7% between these specimens and a M. nigrescens isolate from Canada (18S rRNA gene), suggests that they have diverged genetically, but there are currently no available comparable sequences for the

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Zeng, Jia-Lu, Hui-Xia Chen, Hong-Ru Xu, and Liang Li. "Morphology, complete mitochondrial genome, and molecular phylogeny of Rhabdias macrocephalum n. sp. (Nematoda: Rhabdiasidae) from Diploderma splendidum (Reptilia: Agamidae)." Parasite 31 (2024): 48. http://dx.doi.org/10.1051/parasite/2024046.

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Species of the genus Rhabdias Stiles & Hassall, 1905 are common parasitic nematodes occurring in the lungs of amphibians and reptiles worldwide. In the present study, Rhabdias macrocephalum n. sp. is described using integrated morphological methods (light and scanning electron microscopy) and molecular approaches (sequencing of the nuclear 28S and ITS regions, and mitochondrial cox1, cox2, and 12S genes) based on specimens collected from the green striped tree dragon Diploderma splendidum (Barbour & Dunn) (Reptilia: Agamidae) in China. The complete mitochondrial genome of R. macrocepha

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Thomson, Robin E., Paul B. Frandsen, and Ralph W. Holzenthal. "A preliminary molecular phylogeny of the family Hydroptilidae (Trichoptera): an exploration of combined targeted enrichment data and legacy sequence data." ZooKeys 1111 (July 11, 2022): 467–88. http://dx.doi.org/10.3897/zookeys.1111.85361.

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Hydroptilidae is an extremely diverse family within Trichoptera, containing over 2,600 known species, that displays a wide array of ecological, morphological, and habitat diversity. However, exploration into the evolutionary history of microcaddisflies based on current phylogenetic methods is mostly lacking. The purpose of this study is to provide a proof-of-concept that the use of molecular data, particularly targeted enrichment data, and statistically supported methods of analysis can result in the construction of a stable phylogenetic framework for the microcaddisflies. Here, a preliminary

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Thomson, Robin E., Paul B. Frandsen, and Ralph W. Holzenthal. "A preliminary molecular phylogeny of the family Hydroptilidae (Trichoptera): an exploration of combined targeted enrichment data and legacy sequence data." ZooKeys 1111 (July 11, 2022): 467–88. https://doi.org/10.3897/zookeys.1111.85361.

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Hydroptilidae is an extremely diverse family within Trichoptera, containing over 2,600 known species, that displays a wide array of ecological, morphological, and habitat diversity. However, exploration into the evolutionary history of microcaddisflies based on current phylogenetic methods is mostly lacking. The purpose of this study is to provide a proof-of-concept that the use of molecular data, particularly targeted enrichment data, and statistically supported methods of analysis can result in the construction of a stable phylogenetic framework for the microcaddisflies. Here, a preliminary

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Torres-Vila, L. M., and R. Bonal. "DNA barcoding of large oak-living cerambycids: diagnostic tool, phylogenetic insights and natural hybridization between Cerambyx cerdo and Cerambyx welensii (Coleoptera: Cerambycidae)." Bulletin of Entomological Research 109, no. 05 (2018): 583–94. http://dx.doi.org/10.1017/s0007485318000925.

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AbstractThree large saproxylic cerambycids with different pest/legal status co-occur in the Iberian oak woodlands, Cerambyx welensii (Cw), Cerambyx cerdo (Cc) and Prinobius myardi (Pm): Cw is an emerging pest, Cc is a protected but sometimes harmful species and Pm is a secondary/minor pest. A precise taxonomic diagnosis is necessary for research, management or protection purposes, but may be problematic mainly because Cw and Cc larvae are morphologically indistinguishable. To resolve this constraint, we genotyped adults, larvae and eggs collected over a wide geographical range using the mitoch

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Mengual, Ximo, and Anatolij V. Barkalov. "Two new species of Rohdendorfia (Diptera: Syrphidae) from Central Asia." Acta Entomologica Musei Nationalis Pragae 59, no. 1 (2019): 325–35. http://dx.doi.org/10.2478/aemnp-2019-0025.

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Abstract Two new species of the flower fly genus Rohdendorfia Smirnov, 1924 (Diptera: Syrphidae) are described, Rohdendorfia bella Mengual, sp. nov. and Rohdendorfia khakimovi Barkalov, sp. nov. A full description and images of both new species are given, as well as an identification key for the species of this genus. Molecular sequences from mitochondrial cytochrome c oxidase I (COI) gene and nuclear ribosomal 28S and 18S rRNA genes are also provided for the new species. The studied specimens of Rohdendorfia bella Mengual, sp. nov. are the first record of the genus Rohdendorfia from India and

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McCartin, Luke, Emma Saso, Samuel A. Vohsen, et al. "Nuclear eDNA metabarcoding primers for anthozoan coral biodiversity assessment." PeerJ 12 (November 26, 2024): e18607. http://dx.doi.org/10.7717/peerj.18607.

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The distributions of anthozoan corals are undercharacterized due to their wide bathymetric ranges, occurrences in remote locales, and difficulties of identification from morphology alone. Environmental DNA (eDNA) sequencing promises to be a noninvasive strategy to complement conventional approaches for mapping and monitoring the distribution and biodiversity of coral communities. Primers for eDNA metabarcoding have been designed to amplify nuclear and mitochondrial DNA barcodes in shallow scleractinians and mitochondrial MutS in deep-sea octocorals. However, a comprehensive method for eDNA met

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Do, Thinh Dinh, Dae-Wui Jung, Hyun-Jong Kil, and Chang-Bae Kim. "A report of a new species and new record of Cadlina (Nudibranchia, Cadlinidae) from South Korea." ZooKeys 996 (November 24, 2020): 1–18. http://dx.doi.org/10.3897/zookeys.996.54602.

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Of the four species in the genus Cadlina present in the northwestern Pacific region, C. japonica has been the only species recorded from South Korea. For the purpose of investigating Cadlina in Korean waters, specimens were collected from the Korean East Sea (Sea of Japan) by scuba diving. The radula and morphology of these specimens were examined by stereoscopic and scanning electron microscopy. Based on morphology, three species were identified in Korean waters, including the new species, Cadlina koreanasp. nov., C. umiushi (first record in South Korea), and C. japonica. Cadlina koreanasp. n

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Decker, Peter. "Integrative taxonomic revision of the polymorphic flat-millipede genera Oncocladosoma and Somethus in South Australia (Diplopoda : Polydesmida : Paradoxosomatidae)." Invertebrate Systematics 30, no. 3 (2016): 201. http://dx.doi.org/10.1071/is15047.

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The South Australian members of the flat-millipede genera Oncocladosoma Jeekel, 1985 and Somethus Chamberlin, 1920 are revised using an integrative approach incorporating sequence data and morphology. The partial mitochondrial cytochrome c oxidase subunit I (COI) barcoding gene and partial nuclear ribosomal 28S rRNA were amplified and sequenced for 15 Oncocladosoma specimens and 10 Somethus specimens and the datasets were used for molecular phylogenetic analysis and genetic distance determination. Both morphology and molecular data indicate that all species of Oncocladosoma fall within Somethu

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Untereiner, Wendy A., Valérie Débois, and Françoise A. Naveau. "Molecular systematics of the ascomycete genus Farrowia (Chaetomiaceae)." Canadian Journal of Botany 79, no. 3 (2001): 321–33. http://dx.doi.org/10.1139/b01-009.

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The genus Farrowia D. Hawksworth was established for members of the Chaetomiaceae with Botryotrichum-like anamorphs and long-necked ascomata, but the separation of Farrowia from Chaetomium Kunze has been questioned by mycologists who did not consider these characters to be significant at the generic level. The recent description of a species of Chaetomium with an aleurioconidial anamorph and long-necked ascomata prompted us to explore this question employing molecular characters. We sequenced a portion of the nuclear large ribosomal subunit rRNA gene (28S) of members of the Sordariales includi

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Fajfer, Monika, and Praveen Karanth. "New Morphological and Molecular Data Reveal an Underestimation of Species Diversity of Mites of the Genus Geckobia (Acariformes: Pterygosomatidae) in India." Diversity 14, no. 12 (2022): 1064. http://dx.doi.org/10.3390/d14121064.

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Mites of the genus Geckobia (Acariformes: Pterygosomatidae) are permanent and highly specialised ectoparasites of geckos (Gekkota). We conducted a local study on Geckobia mites associated with the geckos of the family Gekkonidae found mainly in the territory of the Indian Institute of Science’s campus (Bangalore, India). In total, we examined 208 lizards belonging to two genera: Hemidactylus and Cnemaspis. We assessed the prevalence of the mites and identified the preferred site for their infestation. We extended the standard morphological identification of the mite species by using DNA barcod

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Hookabe, Natsumi, Yuma Fujino, Naoto Jimi, and Rei Ueshima. "At the edge of the sea: the supralittoral nemertean, Acteonemertes orientalis sp. nov. (Nemertea: Eumonostilifera: Plectonemertidae) from Japan." Invertebrate Systematics 37, no. 6 (2023): 444–56. http://dx.doi.org/10.1071/is22066.

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Ribbon worms (phylum Nemertea) are found in oceans worldwide; however, only a few inhabit terrestrial, semiterrestrial and freshwater environments. In our study, we describe Acteonemertes orientalis sp. nov., a new species of Plectonemertidae Gibson, 1990 and the first plectonemertid discovered in Japan. The species was found in the supralittoral zone, from which nemerteans have rarely been reported and inhabits areas under rocks, wave-dissipating concrete blocks, and fallen leaves and stocks, along the coast of the Sea of Japan. Multi-locus phylogenetic analyses based on two mitochondrial (16

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McDONAGH, LAURA M., та JAMIE R. STEVENS. "The molecular systematics of blowflies and screwworm flies (Diptera: Calliphoridae) using 28S rRNA, COX1 and EF-1α: insights into the evolution of dipteran parasitism". Parasitology 138, № 13 (2011): 1760–77. http://dx.doi.org/10.1017/s0031182011001089.

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SUMMARYThe Calliphoridae include some of the most economically significant myiasis-causing flies in the world – blowflies and screwworm flies – with many being notorious for their parasitism of livestock. However, despite more than 50 years of research, key taxonomic relationships within the family remain unresolved. This study utilizes nucleotide sequence data from the protein-coding genes COX1 (mitochondrial) and EF1α (nuclear), and the 28S rRNA (nuclear) gene, from 57 blowfly taxa to improve resolution of key evolutionary relationships within the family Calliphoridae. Bayesian phylogenetic

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Li, H. D., J. Zagorski, and M. J. Fournier. "Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 3 (1990): 1145–52. http://dx.doi.org/10.1128/mcb.10.3.1145-1152.1990.

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Repression of an essential nucleolar small nuclear RNA (snRNA) gene of Saccharomyces cerevisiae was shown to result in impaired production of 18S rRNA. The effect, observed for an snRNA species of 128 nucleotides (snR128), was evident within one generation after the onset of SNR128 gene repression and correlated well with depletion of the snRNA. The steady-state mass ratio of 18S RNA to 25S RNA decreased eightfold over the course of the analysis. Results from pulse-chase assays revealed the basis of the imbalance to be underaccumulation of 18S RNA and its 20S precursor. This effect appears to

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Li, H. D., J. Zagorski, and M. J. Fournier. "Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 3 (1990): 1145–52. http://dx.doi.org/10.1128/mcb.10.3.1145.

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Repression of an essential nucleolar small nuclear RNA (snRNA) gene of Saccharomyces cerevisiae was shown to result in impaired production of 18S rRNA. The effect, observed for an snRNA species of 128 nucleotides (snR128), was evident within one generation after the onset of SNR128 gene repression and correlated well with depletion of the snRNA. The steady-state mass ratio of 18S RNA to 25S RNA decreased eightfold over the course of the analysis. Results from pulse-chase assays revealed the basis of the imbalance to be underaccumulation of 18S RNA and its 20S precursor. This effect appears to

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Kou, Qi, Xinzheng Li, Tin-Yam Chan, Ka Hou Chu, and Zhibin Gan. "Molecular phylogeny of the superfamily Palaemonoidea (Crustacea : Decapoda : Caridea) based on mitochondrial and nuclear DNA reveals discrepancies with the current classification." Invertebrate Systematics 27, no. 5 (2013): 502. http://dx.doi.org/10.1071/is13005.

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Palaemonoidea is one of the most speciose superfamilies of Caridea. Since it was established, several classification schemes of Palaemonoidea have been proposed and modified. However, the current classification of Palaemonoidea is still in dispute. In this study, one mitochondrial gene (16S rRNA) and three nuclear genes (histone 3, 18S rRNA and 28S rRNA) were used to explore the phylogenetic relationships among the subgroups of the superfamily Palaemonoidea, including seven families with 25 affiliated genera. Based on the combined data with both maximum likelihood and Bayesian inference analys

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LEE, CHI-WOO, and GI-SIK MIN. "Two new species of Pseudocrangonyx (Amphipoda: Pseudocrangonyctidae) from the hyporheic zones in South Korea." Zootaxa 5433, no. 2 (2024): 249–65. http://dx.doi.org/10.11646/zootaxa.5433.2.5.

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Pseudocrangonyx Akatsuka & Komai, 1922, is the most diverse group of subterranean amphipods in the groundwater communities of Far East Asia and is related to the biogeographic origin and evolution of subterranean amphipod fauna in the region. In this study, we describe two new subterranean species of pseudocrangonyctid amphipods, P. seomjinensis sp. nov. and P. danyangensis sp. nov., from the hyporheic zones in South Korea. These new species can be distinguished from their congeners by the length of the uropod 2 outer ramus, the number of accessory flagella of antenna 1, and telson shape.

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JI, XIAO-HONG, GANG HE, QI-BIAO SUN, and YE CHEN. "Fulvifomes parviungulatus (Hymenochaetales, Basidiomycota), a new species from China based on morphological and molecular evidence." Phytotaxa 641, no. 3 (2024): 219–27. http://dx.doi.org/10.11646/phytotaxa.641.3.4.

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Fulvifomes parviungulatus sp. nov. is described and illustrated from China based on morphological characters and phylogenetic analyses. The sequences of the internal transcribed spacer (ITS) region and nuclear-encoded large subunit rRNA gene (28S) were employed. Fulvifomes parviungulatus is characterized by the perennial, small and ungulate basidiocarps, crusted, smooth pileal surface, 4–5 pores per mm with thick dissepiments, a dimitic hyphal system with interwoven skeletal hyphae, absence of ventricose cystidioles, collapsed hymenium in the studied material and ellipsoid, yellowish brown, th

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Do, Thinh Dinh, Dae-Wui Jung, Hyun-Jong Kil, and Chang-Bae Kim. "A report of a new species and new record of Cadlina (Nudibranchia, Cadlinidae) from South Korea." ZooKeys 996 (November 24, 2020): 1–18. https://doi.org/10.3897/zookeys.996.54602.

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Of the four species in the genus Cadlina present in the northwestern Pacific region, C. japonica has been the only species recorded from South Korea. For the purpose of investigating Cadlina in Korean waters, specimens were collected from the Korean East Sea (Sea of Japan) by scuba diving. The radula and morphology of these specimens were examined by stereoscopic and scanning electron microscopy. Based on morphology, three species were identified in Korean waters, including the new species, Cadlina koreana sp. nov., C. umiushi (first record in South Korea), and C. japonica. Cadlina koreana sp.

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Wang, Q., S. Li, R. Wang, and P. Paquin. "Phylogeographic analysis of Pimoidae (Arachnida: Araneae) inferred from mitochondrial cytochrome c oxidase subunit I and nuclear 28S rRNA gene regions." Journal of Zoological Systematics and Evolutionary Research 46, no. 2 (2008): 96–104. http://dx.doi.org/10.1111/j.1439-0469.2007.00441.x.

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Zagorski, J., D. Tollervey, and M. J. Fournier. "Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs." Molecular and Cellular Biology 8, no. 8 (1988): 3282–90. http://dx.doi.org/10.1128/mcb.8.8.3282-3290.1988.

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A genetic locus is described that specifies two Saccharomyces cerevisiae small nuclear RNAs (snRNAs). The genes specifying the two snRNAs are separated by only 67 base pairs and are transcribed in the same direction. The product RNAs contain 128 and 190 nucleotides and are designated snR128 and snR190, respectively. These RNAs resemble snRNAs of other eucaryotes in nuclear localization and possession of a 5' trimethylguanosine cap. Neither snRNA is related in sequence to previously described vertebrate or yeast snRNAs. Both RNAs exhibit properties consistent with nucleolar organization and hyd

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Zagorski, J., D. Tollervey, and M. J. Fournier. "Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs." Molecular and Cellular Biology 8, no. 8 (1988): 3282–90. http://dx.doi.org/10.1128/mcb.8.8.3282.

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A genetic locus is described that specifies two Saccharomyces cerevisiae small nuclear RNAs (snRNAs). The genes specifying the two snRNAs are separated by only 67 base pairs and are transcribed in the same direction. The product RNAs contain 128 and 190 nucleotides and are designated snR128 and snR190, respectively. These RNAs resemble snRNAs of other eucaryotes in nuclear localization and possession of a 5' trimethylguanosine cap. Neither snRNA is related in sequence to previously described vertebrate or yeast snRNAs. Both RNAs exhibit properties consistent with nucleolar organization and hyd

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Alojayri, G., S. Al-Quraishy, O. B. Mohammed, E. Al-Shaebi, and R. Abdel-Gaber. "Morphomolecular identification of Polylabris lingaoensis Tingbao, Kritsky, and Jun, 2007 infecting the twobar Seabream (Acanthopagrus bifasciatus)." Helminthologia 61, no. 4 (2024): 317–26. https://doi.org/10.2478/helm-2024-0031.

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Summary A total of 40 fish samples of the twobar seabream, Acanthopagrus bifasciatus (Sparidae), were collected from January to June 2023 in the Arabian Gulf (Saudi Arabia). Fish samples were surveyed for ectoparasitic taxa. This study revealed the presence of one monogenetic species in A. bifasciatus gills. Parasitic prevalence and the mean value of intensity were 32.5% and 10, respectively. This parasite species has all the morphological characteristics of Polylabris, a genus erected by Euzet and Cauwet, 1967 (Microcotylidae), and mostly resembles Polylabris lingaoensis Tingbao, Kritsky, and

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