Academic literature on the topic 'Nuclear rDNA'

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Journal articles on the topic "Nuclear rDNA"

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Muscarella, D. E., and V. M. Vogt. "A mobile group I intron from Physarum polycephalum can insert itself and induce point mutations in the nuclear ribosomal DNA of saccharomyces cerevisiae." Molecular and Cellular Biology 13, no. 2 (1993): 1023–33. http://dx.doi.org/10.1128/mcb.13.2.1023.

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Pp LSU3 is a mobile group I intron in the extrachromosomal nuclear ribosomal DNA (rDNA) of Physarum polycephalum. As found for other mobile introns, Pp LSU3 encodes a site-specific endonuclease, I-Ppo, which mediates "homing" to unoccupied target sites in Physarum rDNA. The recognition sequence for this enzyme is conserved in all eucaryotic nuclear rDNAs. We have introduced this intron into a heterologous species, Saccharomyces cerevisiae, in which nuclear group I introns have not been detected. The expression of Pp LSU3, under control of the inducible GAL10 promoter, was found to be lethal as
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Muscarella, D. E., and V. M. Vogt. "A mobile group I intron from Physarum polycephalum can insert itself and induce point mutations in the nuclear ribosomal DNA of saccharomyces cerevisiae." Molecular and Cellular Biology 13, no. 2 (1993): 1023–33. http://dx.doi.org/10.1128/mcb.13.2.1023-1033.1993.

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Pp LSU3 is a mobile group I intron in the extrachromosomal nuclear ribosomal DNA (rDNA) of Physarum polycephalum. As found for other mobile introns, Pp LSU3 encodes a site-specific endonuclease, I-Ppo, which mediates "homing" to unoccupied target sites in Physarum rDNA. The recognition sequence for this enzyme is conserved in all eucaryotic nuclear rDNAs. We have introduced this intron into a heterologous species, Saccharomyces cerevisiae, in which nuclear group I introns have not been detected. The expression of Pp LSU3, under control of the inducible GAL10 promoter, was found to be lethal as
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Holst-Jensen, Arne, Linda M. Kohn, and Trond Schumacher. "Nuclear rDNA Phylogeny of the Sclerotiniaceae." Mycologia 89, no. 6 (1997): 885. http://dx.doi.org/10.2307/3761109.

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Ravel-Chapuis, Patrick, Paul Nicolas, Victor Nigon, Odile Neyret, and Georges Freyssinet. "Extrachromosomal circular nuclear rDNA inEuglena gracilis." Nucleic Acids Research 13, no. 20 (1985): 7529–37. http://dx.doi.org/10.1093/nar/13.20.7529.

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Holst-Jensen, Arne, Linda M. Kohn, and Trond Schumacher. "Nuclear rDNA phylogeny of the Sclerotiniaceae." Mycologia 89, no. 6 (1997): 885–99. http://dx.doi.org/10.1080/00275514.1997.12026859.

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Horton, J. Stephen, and Paul A. Horgen. "Polymorphisms in the nuclear DNA of Achlya species: some taxonomic implications." Canadian Journal of Microbiology 35, no. 12 (1989): 1146–55. http://dx.doi.org/10.1139/m89-190.

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Within the genus Achyla, which belongs to the class of fungi known as the Oomycetes, taxonomic judgments have traditionally been made using a variety of sexual criteria. We have used restriction fragment length polymorphisms as a new taxonomic character to examine intra- and inter-specific variation within this genus. Using a cDNA clone coding for the Achlya 18S rRNA gene as a hybridization probe, a 10-kb fragment of ribosomal DNA (rDNA) from Achlya ambisexualis strain E87 was cloned and then mapped for selected restriction enzyme sites. In Southern blot hybridizations, both this rDNA fragment
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Yu, G. L., and E. H. Blackburn. "Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila." Molecular and Cellular Biology 10, no. 5 (1990): 2070–80. http://dx.doi.org/10.1128/mcb.10.5.2070.

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The macronuclear rRNA genes (rDNA) in the ciliate Tetrahymena thermophila are normally palindromic linear replicons, containing two copies of the replication origin region in inverted orientation. A circular plasmid containing a single Tetrahymena rRNA gene (one half palindrome) joined to a tandem repeat of a 1.9-kilobase (kb) rDNA segment encompassing the rDNA replication origin and known replication control elements was used to transform Tetrahymena macronuclei by microinjection. This plasmid was shown previously to have a replication advantage over the rDNA allele of the recipient cell stra
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Yu, G. L., and E. H. Blackburn. "Amplification of tandemly repeated origin control sequences confers a replication advantage on rDNA replicons in Tetrahymena thermophila." Molecular and Cellular Biology 10, no. 5 (1990): 2070–80. http://dx.doi.org/10.1128/mcb.10.5.2070-2080.1990.

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The macronuclear rRNA genes (rDNA) in the ciliate Tetrahymena thermophila are normally palindromic linear replicons, containing two copies of the replication origin region in inverted orientation. A circular plasmid containing a single Tetrahymena rRNA gene (one half palindrome) joined to a tandem repeat of a 1.9-kilobase (kb) rDNA segment encompassing the rDNA replication origin and known replication control elements was used to transform Tetrahymena macronuclei by microinjection. This plasmid was shown previously to have a replication advantage over the rDNA allele of the recipient cell stra
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Panigrahi, Sunil K., Gagan Deep Jhingan, Indrani Som, Alok Bhattacharya, William A. Petri, and Sudha Bhattacharya. "Promoter Analysis of Palindromic Transcription Units in the Ribosomal DNA Circle of Entamoeba histolytica." Eukaryotic Cell 8, no. 1 (2008): 69–76. http://dx.doi.org/10.1128/ec.00254-08.

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ABSTRACT rRNA genes of Entamoeba histolytica are organized as palindromic ribosomal DNA (rDNA) units (I and II) in a 24.5-kb circle. Although the two rDNAs are identical in sequence, their upstream spacers are completely different. Since the intergenic sequences (IGS) of all rDNA copies in other organisms are conserved and contain transcription regulatory sequences, the lack of sequence conservation in the IGS prompted the question of whether both rDNAs are indeed transcriptionally active. We mapped the transcriptional start points (tsp's) and promoters of the two rDNAs. A 51-bp sequence immed
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Zheng, Xu, Qiao-Cheng Chang, Yan Zhang, et al. "Characterization of the Complete Nuclear Ribosomal DNA Sequences ofParamphistomum cervi." Scientific World Journal 2014 (2014): 1–11. http://dx.doi.org/10.1155/2014/751907.

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Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individualParamphistomum cerviwere determined for the first time. The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2), the 28S rDNA, and the intergenic spacer (IGS) regions, had a length range of 8,493–10,221 bp. The lengths of the investigated 18S, ITS1, 5.8S, ITS2, and 28S rDNA sequences, which were 1,994 bp, 1,293 bp, 157 bp, 286 bp, and 4,186 bp, respectively, did not vary. However, the IGS rDNA sequences had a le
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Dissertations / Theses on the topic "Nuclear rDNA"

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Aime, Mary Catherine. "Generic concepts in the Crepidotaceae as inferred from nuclear large subunit ribosomal DNA sequences, morphology, and basidiospore dormancy patterns." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/32285.

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The Crepidotaceae (Imai) Singer (Basidiomycetes: Agaricales) represents a proposed family of saprophytic fungi containing five agaricoid (Crepidotus, Tubaria, Melanomphalia, Simocybe, Pleurotellus) and four cyphelloid (Episphaeria, Phaeosolenia, Pellidiscus, Chromocyphella) genera. Several contemporary classification systems exist that delegate some or all of these genera to other agaric families. Phylogenetic relationships for the most prevalent genera in the Crepidotaceae were investigated using nuclear large subunit ribosomal DNA (LSU rDNA) sequences. Parsimony analysis of the molecular dat
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Hansen, Mario Jens. "Genotyp-Identifizierung und Wechselwirkungen an zwei Populus-Chimären." Doctoral thesis, Humboldt-Universität zu Berlin, Landwirtschaftlich-Gärtnerische Fakultät, 2005. http://dx.doi.org/10.18452/15385.

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Zwei Populus-Pfropfchimären (MA und AI), die aus P. x canadensis ‘Marilandica’ (M), P. maximowizcii x P. trichocarpa ‘Androscoggin’ (A) and P. nigra L. ’Italica’ (I) aufgebaut sind, wurden für Untersuchungen zur Laub- und Blütenblattentwicklung genutzt. In MA bildet M die äußere Lage (L1) und ihr Derivat, die Epidermis, während die inneren Lagen (L2, L3 etc.) von A gebildet werden. Bei AI stammt die L1 von A und L2, L3 etc. werden von I gebildet. Die genotypisch andersartige Epidermis bedingt bei Periklinal-Chimären morphologische Effekte wie zum Beispiel einen Fruchtknoten in einigen MA-Blüt
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Chemudupati, Mahesh. "Investigating the effects of nuclear envelope proteins on nuclear structure and organization in Aspergillus nidulans." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu148009978216118.

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Oliveira, Mariana Cabral de. "Filogenia de Porphyra spp. (Rhodophyta): sequenciamento do gene nuclear para o RNA da subunidade pequena do ribossomo (rDNA 18S) e estudos morfológicos da fase Conchocelis." Universidade de São Paulo, 1993. http://www.teses.usp.br/teses/disponiveis/41/41132/tde-09082016-164600/.

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O gênero Porphyra (Rhodophyta) apresenta uma considerável importância econômica, sendo extensivamente cultivado e consumido como alimento. O gênero é representado por mais de 70 espécies e apresenta ampla distribuição geográfica, desde regiões tropicais até polares. Sua taxonomia, baseada em poucos caracteres da fase macroscópica do seu ciclo de vida, é ainda bastante problemática. Para tentar entender melhor a taxonomia e a história evolutiva de Porphyra foram utilizadas metodologias de biologia molecular e características da fase conchocelis do ciclo de vida. Verificou-se que caracteres da f
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Albert, Benjamin. "Étude de l'organisation spatiale de la transcription des gènes d'ADN ribosomiques." Toulouse 3, 2011. http://thesesups.ups-tlse.fr/1630/.

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La production des ARN ribosomaux (ARNr) est une étape fondamentale dans la biogenèse des ribosomes et la croissance cellulaire. Elle représente près de 60% de l'activité transcriptionnelle. Cette activité a lieu au sein du nucléole qui est une structure dynamique variant de volume selon la croissance, et conservée dans le monde eucaryote. La production des ARNr et la formation du nucléole sont entièrement dépendantes de l'activité d'un unique complexe multiprotéique : l'ARN polymérase I (ARN pol I). Au cours de ma thèse nous avons étudié le fonctionnement de l'ARN pol I chez la levure de bière
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Silva, Natalia de Sousa Teixeira e. "Dinâmica nucleolar e a herança epigenética dos genes ribossomais." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-11082014-174129/.

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O nucléolo é uma organela subnuclear formada pela atividade transcricional dos genes ribossomais 18S-5.8S-26S (rDNA 45S) e consequente biogênese dos ribossomos. A atividade destes genes resulta na região organizadora do nucléolo (NOR), na forma de uma constrição secundária em cromossomos metafásicos. As constrições secundárias se condensam progressivamente durante a mitose e se descondensam ao final da telófase quando a reestruturação do nucléolo se inicia. Genomas que apresentam mais de um locus de rDNA 45S deve apresentar, obrigatoriamente, pelo menos um par de NORs, enquanto os demais loci
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Fuchs, Maria Cecília Perantoni. "Controle genético e epigenético da expressão heteromórfica de regiões organizadoras do nucléolo em Crotalaria retusa L. (Leguminosae-Papilionoideae)." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-14102009-083128/.

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O presente trabalho teve por objetivo compreender e analisar os mecanismos genéticos e epigenéticos da expressão diferencial de regiões organizadoras do nucléolo - RONs através do estudo de dois acessos (CRT-1 e CRT-2) de Crotalaria retusa. O acesso CRT-1 é uma cultivar, enquanto que o acesso CRT-2 é proveniente de uma população periférica da orla marítima de Ilhéus BA. Por serem temporalmente e espacialmente separados, acredita-se que os acessos foram submetidos a pressões seletivas diferentes, resultando em alterações dos padrões epigenéticos, principalmente nas RONs. Para o desenvolvimento
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Vendrell, Arasa Alexandre. "SCF cdc4 regulates msn2 and msn4 dependent gene expression to counteract hog1 induced lethality." Doctoral thesis, Universitat Pompeu Fabra, 2009. http://hdl.handle.net/10803/7153.

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L'activació sostinguda de Hog1 porta a una inhibició del creixement cel·lular. En aquest treball, hem observat que el fenotip de letalitat causat per l'activació sostinguda de Hog1 és parcialment inhibida per la mutació del complexe SCFCDC4. La inhibició de la mort causada per l'activació sostinguda de Hog1 depèn de la via d'extensió de la vida. Quan Hog1 s'activa de manera sostinguda, la mutació al complexe SCFCDC4 fa que augmenti l'expressió gènica depenent de Msn2 i Msn4 que condueix a una sobreexpressió del gen PNC1 i a una hiperactivació de la deacetilassa Sir2. La hiperactivació de Sir2
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"Phylogenetic Placement of African Cornus: Evidence of Nuclear rDNA." TopSCHOLAR, 1997. http://digitalcommons.wku.edu/stu_hon_theses/25.

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Yi-HuanTsou and 鄒毅寰. "To study the role of nucleolar protein MSP58 in ribosomal DNA (rDNA) transcription." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/07774920202949337834.

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碩士<br>國立成功大學<br>藥理學研究所<br>100<br>MSP58/MCRS1 has been reported to interact with several proteins, such as nucleolar protein p120, Mi-2β; transcription factors Daxx, STRA13, Nrf1, SWI/SNF chromatin remodeling subunit BRG1, and also RNA-binding protein FMR. These findings imply that MSP58 proteins are crucial for transcriptional regulation in nuclei and nucleoli. In this study, we examined the role of MSP58 in the transcriptional modulation of the ribosomal genes. Initially, we generated and characterized the polyclonal antibodies against MSP58. The main localization of MSP58 was consistent in n
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Book chapters on the topic "Nuclear rDNA"

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Meissner, Bärbel, Michael F. Trendelenburg, and Ansgar Hofmann. "Parameters of Xenopus rDNA Transcription in Microinjected Oocytes." In Nuclear Structure and Function. Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0667-2_79.

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Sato, Seiichi, and Yasushi Sato. "Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy." In Immunoelectron Microscopy. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-783-9_23.

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Lyapunova, Nataliya A., Nataliya N. Veiko, and Lev N. Porokhovnik. "Human rDNA Genes: Identification of Four Fractions, Their Functions and Nucleolar Location." In Proteins of the Nucleolus. Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-5818-6_5.

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Hause, B., F. Baldauf, K. Stock, C. Wasternack, and M. Metzlaff. "Molecular Analysis of Mitochondrial DNA and Cloning of its rDNA Fragments from Tomato Cell Suspension Culture." In Metabolism and Enzymology of Nucleic Acids. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-0749-5_19.

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Hemleben, Vera, Roman A. Volkov, Ulrike Zentgraf, and Francisco J. Medina. "Molecular Cell Biology: Organization and Molecular Evolution of rDNA, Nucleolar Dominance, and Nucleolus Structure." In Progress in Botany. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18819-0_5.

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SOLTIS, DOUGLAS E., and PAMELA S. SOLTIS. "Chloroplast DNA and Nuclear rDNA Variation: Insights into Autopolyploid and Allopolyploid Evolution." In Biological Approaches and Evolutionary Trends in Plants. Elsevier, 1990. http://dx.doi.org/10.1016/b978-0-12-402960-6.50012-3.

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Conference papers on the topic "Nuclear rDNA"

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Jiang, B. T., J. W. Hines, and F. Y. Zhao. "Application of Fuzzy Weighted Support Vector Regression to Accidents Condition Monitoring of Nuclear Power Plant." In 2016 24th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/icone24-60983.

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Monitoring changes in important parameters has been suggested as a potentially useful condition monitoring (CM) method for the accidents occurred in nuclear power plants (NPPs). The reactor core power is believed to be an important parameter governing the performance of reactor during transient response. The accurate prediction of reactor behavior and power is very important for nuclear power plant operators, especially during the major severe accident scenarios following an initiating event such as rod ejection accident (REA) or rod drop accident (RDA). REA and RDA together are found to be th
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Heo, Yunseon, and Henry A. Sodano. "Thermo-Responsive Shape Memory Self-Healing Polyurethanes and Composites With Continuous Carbon Fiber Reinforcement." In ASME 2015 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/smasis2015-8916.

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Thermally responsive self-healing polyurethanes (1DA1T, 1.5DA1T, and 2DA1H) with shape memory property were developed and the fully reversible Diels-Alder (DA) and retro Diels-Alder (rDA) reactions were employed for the healing mechanism. The transition temperatures of the DA and rDA reactions were confirmed through a differential scanning calorimetry and the molecular level of analysis on the reversibility and the repeatability between the DA and rDA reactions were completed though a variable temperature proton nuclear magnetic resonance at the reaction temperatures. Also, compact tension spe
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Calabrese, R., and F. Vettraino. "Testing of TRANSURANUS Code for RIA Analysis: The FK-1 NSRR Case." In 18th International Conference on Nuclear Engineering. ASMEDC, 2010. http://dx.doi.org/10.1115/icone18-29098.

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Reactivity Initiated Accident (RIA) leads to an unwanted increase in fission rate and power in a region of the reactor core confined around the position of occurrence. The power excursion due to such events may cause fuel rods failures and a subsequent release of radioactive material into the primary coolant of reactor, in severe cases, this release could damage nearby fuel assemblies. In nuclear power plants, RIAs are due to control system faults, e. g. control elements ejection/insertion, or rapid changes in temperature or pressure of moderator. In Boiling Water Reactors (BWRs), the control
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