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Relevant bibliographies by topics / Nuclear Reprogramming

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Academic literature on the topic 'Nuclear Reprogramming'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

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Journal articles on the topic "Nuclear Reprogramming"

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Tada, Takashi, Hironobu Kimura, and Masako Tada. ""Nuclear Reprogramming" and "Epigenetic Reprogramming"." Journal of Mammalian Ova Research 21, no. 3 (2004): 97–104. http://dx.doi.org/10.1274/jmor.21.97.

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Smallridge, Rachel. "Nuclear reprogramming." Nature Reviews Molecular Cell Biology 5, no. 11 (November 2004): 870. http://dx.doi.org/10.1038/nrm1537.

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Halley-Stott, R. P., V. Pasque, and J. B. Gurdon. "Nuclear reprogramming." Development 140, no. 12 (May 28, 2013): 2468–71. http://dx.doi.org/10.1242/dev.092049.

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Ooi, Jolene, and Pentao Liu. "Delineating nuclear reprogramming." Protein & Cell 3, no. 5 (March 31, 2012): 329–45. http://dx.doi.org/10.1007/s13238-012-2920-x.

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Kono, T. "Nuclear transfer and reprogramming." Reviews of Reproduction 2, no. 2 (May 1, 1997): 74–80. http://dx.doi.org/10.1530/revreprod/2.2.74.

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Kono, T. "Nuclear transfer and reprogramming." Reviews of Reproduction 2, no. 2 (May 1, 1997): 74–80. http://dx.doi.org/10.1530/ror.0.0020074.

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Goding, Colin R., Duanqing Pei, and Xin Lu. "Cancer: pathological nuclear reprogramming?" Nature Reviews Cancer 14, no. 8 (July 17, 2014): 568–73. http://dx.doi.org/10.1038/nrc3781.

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Yamanaka, Shinya. "Pluripotency and nuclear reprogramming." Philosophical Transactions of the Royal Society B: Biological Sciences 363, no. 1500 (March 28, 2008): 2079–87. http://dx.doi.org/10.1098/rstb.2008.2261.

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Embryonic stem cells are promising donor cell sources for cell transplantation therapy, which may in the future be used to treat various diseases and injuries. However, as is the case for organ transplantation, immune rejection after transplantation is a potential problem with this type of therapy. Moreover, the use of human embryos presents serious ethical difficulties. These issues may be overcome if pluripotent stem cells are generated from patients' somatic cells. Here, we review the molecular mechanisms underlying pluripotency and the currently known methods of inducing pluripotency in somatic cells.

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Lorthongpanich, Chanchao, Davor Solter, and Chin Yan Lim. "Nuclear reprogramming in zygotes." International Journal of Developmental Biology 54, no. 11-12 (2010): 1631–40. http://dx.doi.org/10.1387/ijdb.103201cl.

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Hochedlinger, Konrad, and Rudolf Jaenisch. "Nuclear reprogramming and pluripotency." Nature 441, no. 7097 (June 2006): 1061–67. http://dx.doi.org/10.1038/nature04955.

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Dissertations / Theses on the topic "Nuclear Reprogramming"

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Koziol, Magdalena Justyna. "Identification of nuclear reprogramming factors." Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613159.

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McClellan, Michael J. "Cellular reprogramming by Epstein-Barr virus nuclear antigens." Thesis, University of Sussex, 2015. http://sro.sussex.ac.uk/id/eprint/54308/.

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Epstein-Barr virus (EBV) is a widespread human B cell virus that is linked to many malignancies. EBV modulates the transcriptome of B lymphocytes to drive immortalisation and viral persistence. This is primarily coordinated by the EBV nuclear antigens (EBNA) 2 and the EBNA 3 family (3A, 3B and 3C), which regulate overlapping sets of cellular genes. Using Chromatin immunoprecipitation (ChIP) coupled to next generation sequencing we found >21000 EBNA 2 and >7000 EBNA 3 binding sites in the human genome, providing the first evidence of EBNA 3 association with the human genome in vivo. Binding sites were predominantly distal to transcription start sites (TSS) indicating a key role in long-range gene control. This was especially pronounced for EBNA 3 proteins (84% of sites over 4kb from any TSS). 56% of genes previously reported to be regulated by these EBNA proteins in micro array experiments were bound by an EBNA. Using ChIP-QPCR we confirmed EBNA 3C bound to and promoted epigenetic silencing of a subset of integrin receptor signalling genes (ITGA4, ITGB1, ADAM28, ADAMDEC1). Indirect silencing of CXCL10 and CXCL11 chemokines by EBNA 3C was also demonstrated. 75% of sites bound by EBNA 3 were also bound by EBNA 2 implicating extensive interplay between EBNA proteins in gene regulation. By examining novel (WEE1, CTBP2) and known (BCL2L11, ITGAL) targets of EBNA 3 proteins bound at promoter-proximal or distal binding sites, we found both cell-type and locus-specific binding and transcriptional regulation. Importantly, genes differentially regulated by a subset EBNA 3 proteins were bound by the same subset, providing a mechanism for selective regulation of host genes by EBNA 3 proteins. In summary, this research demonstrates that EBNA proteins primarily act through long-range enhancer elements and regulate gene expression in a locus and gene-specific manner through differential binding.

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Greggains, Gareth David. "Cell cycle regulation and nuclear reprogramming in mammalian oocytes." Thesis, University of Newcastle Upon Tyne, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538926.

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Eggan, Kevin C. (Kevin Carl) 1974. "Cloning, stem cells and epigenetic reprogramming after nuclear transfer." Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/29931.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2003.
Includes bibliographical references (leaves 128-146).
The process by which a single totipotent cell becomes a complex organism is a unidirectional program, with each mitotic division generating new cells that gradually differentiate towards more specified fates and specialized functions. Nuclear transfer (NT) experiments have demonstrated the epigenetic nature of development and showed, that although differentiated cells have a very limited developmental potential, the nuclei of these cells retain the potency to direct embryogenesis after reintroduction into the unfertilized oocyte. Herein, we have used the mouse as a model system for understanding both the nature of epigenetic reprogramming that occurs after NT as well as the ramifications it has for the development of cloned animals. Specifically, we investigated how epigenetic states are reprogrammed after NT and demonstrated that the inactive X chromosome is reactivated in NT embryos, resulting in normal X inactivation in female clones. Additionally, investigations into the factors that influence the survival of cloned animals, indicate that there are considerable genetic influences on the cloning process. These genetic factors modify the survival of mice cloned from ES cells by influencing the developmental potential of the donor ES cells rather then the reprogramming process itself. This realization has subsequently led to the development of novel methods for the expedited production of complex mutant mice, which are also described. Finally, we have created cloned embryos by NT from both cortical and mature olfactory sensory neurons to address question of nuclear equivalence in the brain and to investigate whether generation of synaptic diversity or odorant receptor choice, are mediated by genetic as well as epigenetic events.
by Kevin C. Eggan.
Ph.D.

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Sullivan, Stephen. "Assessment of nuclear reprogramming activity in mammalian ES cells." Thesis, University of Edinburgh, 2004. http://hdl.handle.net/1842/27486.

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A murine cell hybrid system was established and optimised to study nuclear reprogramming of somatic cells via fusion with embryonic stem cells. The system generated hybrid clones which displayed an ES phenotype and in which a somatically derived transgenic Oct-4 promoter had been reactivated. Methods were also developed to quantify heterokaryon formation, so that effects on the fusion process could be distinguished from effects on nuclear reprogramming per se. Hybrid cell lines displayed high endogenous alkaline phosphatase activity and expressed undifferentiated cell marker SSEA-1 but not markers associated with differentiated cells (SSEA-4 and CD90). These lines were pluripotent, demonstrating the ability to form the three embryonic lineages both in vivo and in vitro. This system was used to investigate whether several treatments (all either known or expected to perturb global gene expression patterns) affected nuclear reprogramming. Moderate heat shocking of thymocytes prior to fusion with murine ES cells resulted in increased hybridisation frequencies but, as fusion was also increased, it was impossible to verify whether an increase in nuclear reprogramming was partly responsible. Serum starvation of primary embryonic fibroblasts significantly increased nuclear reprogramming, as did ES cell confluence. Murine ES cells were found to lose their capacity to reprogram as they reached high passage numbers. Constitutive or transient expression of nucleoplasmin in murine ES cells did not increase their capacity to reprogram but instead led to increased cell death. Attempts were made to generate hybrids from human ES cells, but no hybrids were successfully generated. This was at least partly due to human ES cells being more difficult to fuse with other cells even using a range of different fusagens. Finally it was found that treating human ES cells with hyaluronidase prior to electropulsing resulting in five times more heterokaryon formation indicating that the extracellular matrix of these cells had prevented fusion. The methods developed here provide the basis for further study of the mechanisms underpinning reprogramming.

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Byrne, J. A. "Nuclear transfer, nuclear reprogramming and the delivery of exogenous macromolecules into living amphibian cells." Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597205.

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This thesis describes a number of foundation studies regarding Xenopus nuclear transfer, inter-species transcriptional reprogramming and a method for delivering exogenous macromolecules into living amphibian cells. I divided my research into four stages. First, I demonstrated that morphologically and reproductively normal cloned animals could be obtained following Xenopus nuclear transfer using streptolysin permeabilised donor cells. The use of streptolysin, as opposed to the traditional cell-squashing method, permits more consistent, more controlled and gentler donor cell permeabilisation, and makes nuclear transfer quicker and technically easier to perform. Second, I demonstrated that epigenetically aberrant and developmentally defective cloned Xenopus embryos possess cells that retain the capacity to differentiate into multiple cell types, survive for an extended period of time and exhibit a normal growth morphology. Third, I demonstrated that Xenopus oocytes could transcriptionally reprogram mammalian somatic cell nuclei to express Oct-4, a mammalian stem cell/pluripotency marker. Finally, I demonstrated that the reversible streptolysin permeabilisation technique, previously described for mammalian cell lines, could be modified slightly and then used with an amphibian cell line to deliver exogenous macromolecules into living amphibian cells. This modified cell delivery technique has a wide variety of potential reprogramming, transgenesis and differentiation applications for researchers working with amphibian cells. In my opinion, the long-term objectives of the reprogramming/cloning field are to identify the reprogramming molecular mechanisms, to improve the efficiency of nuclear transfer and to obtain therapeutically useful isogenic human embryonic stem cells. My research provides a number of foundation result on which these larger problems can be addressed in the future. First, my result with developmentally defective cloned Xenopus embryos provides a strong case for future research to investigate whether isogenic embryonic stem cells can be obtained from developmentally defective cloned primate/human embryos; stem cells that could potentially be used to treat various degenerative diseases.

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Pérez, Camps Mireia. "Epigenetic reprogramming of somatic cells by nuclear transplant in zebrafish." Doctoral thesis, Universitat Politècnica de València, 2010. http://hdl.handle.net/10251/6902.

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El estudio de los mecanismos de reprogramación nuclear tiene actualmente una notable importancia, dado que el dominio de estos procesos constituyen la clave para actuar eficazmente en cuestiones tan dispares como el cáncer o la medicina regenerativa. También resulta muy importante este tipo de estudios sobre reprogramación cuando se pretende la obtención de animales transgénicos múltiples y orientados. Aunque para ello se pueden utilizar muy diversos modelos animales, en nuestro caso, se ha optado por el pez cebra, por sus características en el desarrollo, como la brevedad en la embriogénesis y transparencia de los embriones, su capacidad de regeneración y el conocimiento de su genoma, entre otras. Bien es cierto que a estas ventajas le acompañan cierto inconvenientes tales como no disponer hasta este momento de técnicas tales como el transplante nuclear y, a otro nivel, el quimerismo. Técnicas cuyos desarrollo se pretende en esta tesis, lo que justifica los objetivos aquí planteados. Para ello se han realizado diferentes trabajos experimentales titulados: "Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish", "Evaluation of presumptive caudal fin blastema cells as candidate donors in intraspecies zebrafish (Danio rerio) chimaeras", "Definition of three somatic adult cell nuclear transplant methods in zebrafish (Danio rerio): before, during and after egg activation by sperm fertilization", "Transplant of adult fibroblast into the central region of metaphase II eggs resulted in mid blastula transition (MBT) embryos", "Electroactivation of zebrafish (Danio rerio) eggs", "Comparison of different activating stimuli efficiency in zebrafish nuclear transplant", "Reconstruction of heteroparental gynogenetic diploid condition by nuclear transplant in zebrafish". Los dos primeros relativos al quimerismo, su eficiencia final se optimizó mediante la penalización con radiación UV del embrión receptor.
Pérez Camps, M. (2009). Epigenetic reprogramming of somatic cells by nuclear transplant in zebrafish [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/6902
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8

Kuo, Yen-Hsi. "Using Xenopus oocyte as a model system for nuclear reprogramming studies." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612393.

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Köhler, Daniela. "Cloning in cattle : nuclear architecture and epigenetic status of chromatin during reprogramming of donor cell nuclei." kostenfrei, 2008. http://edoc.ub.uni-muenchen.de/9915/.

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Pawlak, Mathias. "Stem cells, differentiation and nuclear reprogramming : the roles of Klf4 and geminin /." Heidelberg, 2008. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000259539.

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Books on the topic "Nuclear Reprogramming"

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Hu, Kejin, ed. Nuclear Reprogramming. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1084-8.

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Beaujean, Nathalie, Hélène Jammes, and Alice Jouneau, eds. Nuclear Reprogramming. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-1594-1.

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Steve, Pells. Nuclear Reprogramming. New Jersey: Humana Press, 2005. http://dx.doi.org/10.1385/1597450057.

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Ainscough, Justin. Nuclear Reprogramming and Stem Cells. Totowa, NJ: Springer Science+Business Media, LLC, 2011.

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Beaujean, Nathalie, Hélène Jammes, and Alice Jouneau. Nuclear reprogramming: Methods and protocols. New York: Humana Press, 2014.

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Ainscough, Justin, Shinya Yamanaka, and Takashi Tada, eds. Nuclear Reprogramming and Stem Cells. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-225-0.

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Bock, Gregory, and Jamie Goode, eds. Stem Cells: Nuclear Reprogramming and Therapeutic Applications. Chichester, UK: John Wiley & Sons, Ltd, 2005. http://dx.doi.org/10.1002/0470091452.

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Steve, Pells, ed. Nuclear reprogramming: Methods and protocols. Totowa, N.J: Humana Press, 2006.

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Tada, Takashi, Justin Ainscough, and Shinya Yamanaka. Nuclear Reprogramming and Stem Cells. Humana, 2014.

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Nuclear reprogramming: Methods and protocols. Totowa, NJ: Humana Press, 2006.

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Book chapters on the topic "Nuclear Reprogramming"

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Tada, Takashi. "Nuclear Reprogramming." In Methods in Molecular Biology, 229–36. Totowa, NJ: Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-154-3_15.

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Ross, Pablo J., and Jose B. Cibelli. "Bovine Somatic Cell Nuclear Transfer." In Cellular Programming and Reprogramming, 155–77. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-691-7_10.

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Docherty, Kevin. "Reprogramming Towards Pancreatic β-Cells." In Nuclear Reprogramming and Stem Cells, 177–91. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-225-0_14.

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Alberio, Ramiro, and Andrew D. Johnson. "Epigenetic Reprogramming with Oocyte Molecules." In Nuclear Reprogramming and Stem Cells, 45–57. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-225-0_5.

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Gurdon, J. B., J. A. Byrne, and S. Simonsson. "Nuclear Reprogramming by Xenopus Oocytes." In Stem Cells: Nuclear Reprogramming and Therapeutic Applications, 129–41. Chichester, UK: John Wiley & Sons, Ltd, 2008. http://dx.doi.org/10.1002/0470091452.ch11.

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Mitalipov, Shoukhrat, and Don Wolf. "Totipotency, Pluripotency and Nuclear Reprogramming." In Engineering of Stem Cells, 185–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/10_2008_45.

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Eminli, S., R. Jaenisch, and K. Hochedlinger. "Strategies to Induce Nuclear Reprogramming." In Cancer Stem Cells, 83–98. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/2789_2007_045.

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Prather, Randall S. "Nuclear Modifications and Reprogramming After Nuclear Transfer." In Assisted Fertilization and Nuclear Transfer in Mammals, 227–38. Totowa, NJ: Humana Press, 2001. http://dx.doi.org/10.1007/978-1-59259-369-9_14.

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Lim, Ai Khim, Barbara B. Knowles, Toshie Kai, and Daniel M. Messerschmidt. "Inherent Nuclear Reprogramming in Mammalian Embryos." In Nuclear Reprogramming and Stem Cells, 7–24. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-225-0_3.

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Gurdon, John, and Azim Surani. "Introduction." In Nuclear Reprogramming and Stem Cells, 1–2. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-225-0_1.

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Conference papers on the topic "Nuclear Reprogramming"

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Cai, Guang-bo, Ya-dong Zhang, Yu-xiang Han, and Jia-pei Yu. "Analyzing the Impact of Solutes on PKA Spectrum for Simulation of Neutron Induced-Radiation Damage in Zr-Based Metals." In 2018 26th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/icone26-82132.

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Radiation-induced damage and degradation to shielding and structure materials in nuclear reactors is one of the key limiting factors that affect the safety considerations and the lifetime. Neutron radiation damages materials mainly by exciting a number of Primary Knock-on Atom (PKAs). PKAs induce displacement cascades, causing microstructure changes and mechanical degradations in materials. Computer simulations are used to model this complex process. Knowing the PKA spectrum accurately is important because PKA spectrum and the input of computer simulation are coupled. In this work, we aim to obtain the PKA spectrum in Zr-based alloys with relatively low Zr concentration by using the Geant4 software. Some new functions were added by reprogramming in Geant4. We found that the energy spectra of PKA in Zr2Cu and Zr2Ni are mainly caused by Zr atom, and the shape of the average energy spectra are similar with pure Zr. The number of PKA distributions and the energy deposition in these two Zr-based metals are similar with pure Zr but different than those in pure Ni. These finding indicate that the metallic elements of Cu and Ni have small impact on PKA spectrum in Zr-based alloys, which illustrate that simplified simulation models are feasible when using computer for simulating. Moreover, it has great significant for the calculation of irradiation damage and the computer simulation for the process of collision cascade after one PKA is formed.

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Sengupta, Surojeet, Shuait Nair, Lu Jin, Catherine M. Sevigny, Brandon Jones, and Robert Clarke. "Abstract PS17-50: Nuclear expression of acetyl-CoA producing enzymes and their roles in epigenetic reprogramming in breast cancer cells." In Abstracts: 2020 San Antonio Breast Cancer Virtual Symposium; December 8-11, 2020; San Antonio, Texas. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.sabcs20-ps17-50.

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Konno, Masamitsu. "Abstract 5323: Innovative bridged nucleic acid (BNA)-based cellular reprogramming medicine towards extermination of gastrointestinal cancer." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-5323.

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Hwang, William L., Karthik A. Jagadeesh, Jimmy A. Guo, Hannah I. Hoffman, Eugene Drokhlyansky, Nicholas Van Wittenberghe, Samouil Farhi, et al. "Abstract PR-007: Single-nucleus and spatial transcriptomics of archival pancreatic ductal adenocarcinoma reveals multi-compartment reprogramming after neoadjuvant treatment." In Abstracts: AACR Virtual Special Conference on Pancreatic Cancer; September 29-30, 2020. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.panca20-pr-007.

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