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Relevant bibliographies by topics / Nuclear run-off analysis; Termination

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Academic literature on the topic 'Nuclear run-off analysis; Termination'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

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Journal articles on the topic "Nuclear run-off analysis; Termination"

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Kerppola, T. K., and C. M. Kane. "Intrinsic sites of transcription termination and pausing in the c-myc gene." Molecular and Cellular Biology 8, no. 10 (1988): 4389–94. http://dx.doi.org/10.1128/mcb.8.10.4389.

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We have studied transcription elongation and termination in the human c-myc gene. Transcription of c-myc gene sequences with purified mammalian RNA polymerase II revealed several sites of transcription termination and pausing in the vicinity of the exon 1-intron 1 junction. This region previously has been shown to block transcription elongation in vivo by nuclear run-on analysis (D. Bentley and M. Groudine, Nature [London] 321:702-706, 1986). These sites were recognized by purified RNA polymerase II, and we therefore designated them intrinsic sites of termination and pausing. Two of these site

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Kerppola, T. K., and C. M. Kane. "Intrinsic sites of transcription termination and pausing in the c-myc gene." Molecular and Cellular Biology 8, no. 10 (1988): 4389–94. http://dx.doi.org/10.1128/mcb.8.10.4389-4394.1988.

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We have studied transcription elongation and termination in the human c-myc gene. Transcription of c-myc gene sequences with purified mammalian RNA polymerase II revealed several sites of transcription termination and pausing in the vicinity of the exon 1-intron 1 junction. This region previously has been shown to block transcription elongation in vivo by nuclear run-on analysis (D. Bentley and M. Groudine, Nature [London] 321:702-706, 1986). These sites were recognized by purified RNA polymerase II, and we therefore designated them intrinsic sites of termination and pausing. Two of these site

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Birkenheuer, Claire H., Charles G. Danko, and Joel D. Baines. "Herpes Simplex Virus 1 Dramatically Alters Loading and Positioning of RNA Polymerase II on Host Genes Early in Infection." Journal of Virology 92, no. 8 (2018): e02184-17. http://dx.doi.org/10.1128/jvi.02184-17.

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ABSTRACTHerpes simplex virus 1 (HSV-1) transcription is mediated by cellular RNA polymerase II (Pol II). Recent studies investigating how Pol II transcription of host genes is altered after HSV-1 are conflicting. Chromatin immunoprecipitation sequencing (ChIP-seq) studies suggest that Pol II is almost completely removed from host genes at 4 h postinfection (hpi), while 4-thiouridine (4SU) labeling experiments show that host transcription termination is extended at 7 hpi, implying that a significant amount of Pol II remains associated with host genes in infected cells. To address this discrepan

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Miller, H., C. Asselin, D. Dufort, et al. "A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block." Molecular and Cellular Biology 9, no. 12 (1989): 5340–49. http://dx.doi.org/10.1128/mcb.9.12.5340.

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A block to elongation of transcription has been shown to occur within the first exon of the human and murine c-myc genes. The extent of this block was found to vary with the physiological state of cells, indicating that modulation of the transcriptional block can serve to control the expression of this gene. To determine which sequences are required in cis for the transcriptional block, we generated a series of constructs containing various portions of murine c-myc 5'-flanking and exon 1 sequences. We established populations of HeLa and CV-1 cells stably transfected with these constructs. The

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Miller, H., C. Asselin, D. Dufort, et al. "A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block." Molecular and Cellular Biology 9, no. 12 (1989): 5340–49. http://dx.doi.org/10.1128/mcb.9.12.5340-5349.1989.

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A block to elongation of transcription has been shown to occur within the first exon of the human and murine c-myc genes. The extent of this block was found to vary with the physiological state of cells, indicating that modulation of the transcriptional block can serve to control the expression of this gene. To determine which sequences are required in cis for the transcriptional block, we generated a series of constructs containing various portions of murine c-myc 5'-flanking and exon 1 sequences. We established populations of HeLa and CV-1 cells stably transfected with these constructs. The

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Napp, Adriane E., Torsten Diekhoff, Olf Stoiber, et al. "Audio-guided self-hypnosis for reduction of claustrophobia during MR imaging: results of an observational 2-group study." European Radiology 31, no. 7 (2021): 4483–91. http://dx.doi.org/10.1007/s00330-021-07887-w.

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Abstract Objectives To evaluate the influence of audio-guided self-hypnosis on claustrophobia in a high-risk cohort undergoing magnetic resonance (MR) imaging. Methods In this prospective observational 2-group study, 55 patients (69% female, mean age 53.6 ± 13.9) used self-hypnosis directly before imaging. Claustrophobia included premature termination, sedation, and coping actions. The claustrophobia questionnaire (CLQ) was completed before self-hypnosis and after MR imaging. Results were compared to a control cohort of 89 patients examined on the same open MR scanner using logistic regression

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Wu, Yanping, Jennifer H. McQuiston, Andrew Cox, Todd D. Pack, and Thomas J. Inzana. "Molecular Cloning and Mutagenesis of a DNA Locus Involved in Lipooligosaccharide Biosynthesis in Haemophilus somnus." Infection and Immunity 68, no. 1 (2000): 310–19. http://dx.doi.org/10.1128/iai.68.1.310-319.2000.

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ABSTRACT Haemophilus somnus undergoes antigenic and structural phase variation in its lipooligosaccharide (LOS). A gene (lob-1) containing repetitive 5′-CAAT-3′ sequences that may, in part, contribute to phase variation was cloned and sequenced (T. J. Inzana et al., Infect. Immun. 65:4675–4681, 1997). We have now identified another putative gene (lob-2A) immediately upstream from lob-1. Lob-2A contained homology to several LOS biosynthesis proteins of the family Pasteurellaceae and the LgtB and LgtE galactosyltransferases of Neisseria meningitidis and N. gonorrhoeae. Unlikelob-1, lob-2A contai

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Martínez-Calvillo, Santiago, Dan Nguyen, Kenneth Stuart, and Peter J. Myler. "Transcription Initiation and Termination on Leishmania major Chromosome 3." Eukaryotic Cell 3, no. 2 (2004): 506–17. http://dx.doi.org/10.1128/ec.3.2.506-517.2004.

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ABSTRACT Genome projects involving Leishmania and other trypanosomatids have revealed that most genes in these organisms are organized into large clusters of genes on the same DNA strand. We have previously shown that transcription of the entire Leishmania major Friedlin (LmjF) chromosome 1 (chr1) initiates bidirectionally between two divergent gene clusters. Here, we analyze transcription of LmjF chr3, which contains two convergent clusters of 67 and 30 genes, separated by a tRNA gene, with a single divergent protein-coding gene located close to the “left” telomere. Nuclear run-on analyses in

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Shiota, Chiyo, Kazuhiro Ikenaka, and Katsuhiko Mikoshiba. "Developmental Expression of Myelin Protein Genes in Dysmyelinating Mutant Mice: Analysis by Nuclear Run-Off Transcription Assay, In Situ Hybridization, and Immunohistochemistry." Journal of Neurochemistry 56, no. 3 (1991): 818–26. http://dx.doi.org/10.1111/j.1471-4159.1991.tb01997.x.

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Falk, G., and J. E. Walker. "Transcription of Rhodospirillum rubrum atp operon." Biochemical Journal 229, no. 3 (1985): 663–68. http://dx.doi.org/10.1042/bj2290663.

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The photosynthetic non-sulphur bacterium Rhodospirillum rubrum contains a cluster of five genes encoding the subunits of F1-ATPase [Falk, Hampe & Walker (1985) Biochem. J. 228, 391-407]. Transcription of these genes has been studied by two methods, transcriptional mapping with S1 nuclease and primer extension analysis. Thereby a 5'-end in RNA derived from this region has been demonstrated at a guanine residue 236 bases before the initiation codon of the gene for the delta-subunit, the first in this cluster. DNA sequences on the 5' side of this nucleotide show some similarity to promoters i

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Dissertations / Theses on the topic "Nuclear run-off analysis; Termination"

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White, Hilary Louise. "Transcription in the human #beta# globin locus." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337833.

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Book chapters on the topic "Nuclear run-off analysis; Termination"

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George, Laurent, and Pierre Courbin. "Reconfiguration of Uniprocessor Sporadic Real-Time Systems." In Reconfigurable Embedded Control Systems. IGI Global, 2011. http://dx.doi.org/10.4018/978-1-60960-086-0.ch007.

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In this chapter the authors focus on the problem of reconfiguring embedded real-time systems. Such reconfiguration can be decided either off-line to determine if a given application can be run on a different platform, while preserving the timeliness constraints imposed by the application, or on-line, where a reconfiguration should be done to adapt the system to the context of execution or to handle hardware or software faults. The task model considered in this chapter is the classical sporadic task model defined by a Worst Case Execution Time (WCET), a minimum inter-arrival time (also denoted the minimum Period) and a late termination deadline. The authors consider two preemptive scheduling strategies: Fixed Priority highest priority first (FP) and Earliest Deadline First (EDF). They propose a sensitivity analysis to handle reconfiguration issues. Sensitivity analysis aims at determining acceptable deviations from the specifications of a problem due to evolutions in system characteristics (reconfiguration or performance tuning). They present a state of the art for sensitivity analysis in the case of WCETs, Periods and Deadlines reconfigurations and study to what extent sensitivity analysis can be used to decide on the possibility of reconfiguring a system.

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Conference papers on the topic "Nuclear run-off analysis; Termination"

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Leung, W. H. "On the MEGAPIE Target Thermal Hydraulics: A RELAP5 Analysis." In 12th International Conference on Nuclear Engineering. ASMEDC, 2004. http://dx.doi.org/10.1115/icone12-49378.

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A new type of liquid-metal target is designed for the Spallation Neutron Source in PSI. LBE is selected to be the target material and the primary coolant as well. RELAP5/MOD 3.2 is used to analyze the system thermal hydraulics. The nominal conditions are chosen based on temperature constraints from the design assessments. The steady state results are in the proximity of the design specifications and the heat removal capacity is adequately deployed. The normal thermal hydraulic transients, namely the proton beam and beam interrupt, are studied. A basic PID (Proportional, Integral, Derivative) c

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Duan, Tianying, Peide Zhou, Bin Long, et al. "Preliminary Research and Design in the Nuclear Island Systems of 1500MWt Standing-Wave Reactor." In 2013 21st International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/icone21-16134.

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As one kind of fast reactor, the Traveling-Wave Reactor (TWR) utilizes depleted uranium with a small amount of enriched uranium/ plutonium which is used to kick off the chain reaction. The TWR can run for decades without refueling or removing any used fuel from the reactor. The most challenging issues on TWR are fuel design, structural material for fuel cladding, core physics process analysis and core physics design. Based on the present technology of fuel and structural material, a new concept named Standing-Wave Reactor (SWR) which is the preliminary stage of the TWR is proposed. The wave of

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Colón Rodríguez, José, Nor Farida Harun, Nana Zhou, Edward Sabolsky, and David Tucker. "System Analysis of a 100kW Internal Combustion Engine (ICE) Solid Oxide Fuel Cell (SOFC) Hybrid Configuration." In ASME 2020 Power Conference collocated with the 2020 International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/power2020-16620.

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Abstract Due to the intermittent nature of the renewable power plants and the rigid operation of existing plans, the need for flexible power production is eminent. Hybrid energy systems have shown potential for flexible power production capable to fulfill the power demands and maintain the efficiency. This work studies different design cases of a 100kW Internal Combustion Engine (ICE) and Solid Oxide Fuel Cell (SOFC) hybrid system. Anode off-gas from the fuel cell stack provided the chemical energy to run the ICE. Heat management of the anode exhaust was considered to attain the operational li

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Yamamoto, Shinji, and Toshiro Kitamura. "Best Practices in Japan of Human Resource Development for NPP O&M: Roles and Lessons From Training Centers." In 16th International Conference on Nuclear Engineering. ASMEDC, 2008. http://dx.doi.org/10.1115/icone16-48137.

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The use of best practices and their lateral expansion as a benchmark is one of effective methods of “knowledge management (NK)”. Best practices of human resources development were collected (selected examples are listed below) from all 11 training centers annexed to the nuclear power plants in Japan and lessons were learned for possible lateral development for improving other stakeholders’ NK. Such best practices will provide productive information for designing their own human resources development strategies. Examples of collected good practices: • Exhibition of troubles and negative legacie

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Hawila, Mohammad A., and Karen Vierow Kirkland. "RCIC Turbo-Pump Scaling Through CFD and Model Testing for the Texas A&M University NHTS Facility." In 2018 26th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/icone26-81119.

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One of the requirements for licensing a nuclear power plant in the U.S is the capability to survive and recover from a station blackout according to the U.S Nuclear Regulatory Commission (USNRC). Station blackout is the loss of all off-site and onsite power simultaneously. Therefore, experimental test facilities are being constructed and operated to test the performance of the related safety systems in a nuclear power plant. Design and construction of a test facility creates the need to perform scaling analysis to ensure proper representation of key components and phenomena of interest. One of

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Virgen, Matthew Miguel, and Fletcher Miller. "Analysis of a Combined Cycle Plant Using a Small Particle Receiver to Drive a Primary Brayton Cycle." In ASME 2015 9th International Conference on Energy Sustainability collocated with the ASME 2015 Power Conference, the ASME 2015 13th International Conference on Fuel Cell Science, Engineering and Technology, and the ASME 2015 Nuclear Forum. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/es2015-49317.

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All current commercial CSP plants operate at relatively low thermodynamic efficiency due to lower temperatures than similar conventional plants and due to the fact that they all employ Rankine conversion cycles. We present here an investigation on the effects of adding a bottoming steam power cycle to a hybrid CSP plant based on a Small Particle Heat Exchange Receiver (SPHER) driving a gas turbine as the primary cycle. Due to the high operating temperature of the SPHER being considered (over 1000 Celsius), the exhaust air from the primary Brayton cycle still contains a tremendous amount of exe

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Cumpston, J., and John D. Pye. "Optimisation of Paraboloidal Dish Fields for Direct-Steam Generation." In ASME 2015 9th International Conference on Energy Sustainability collocated with the ASME 2015 Power Conference, the ASME 2015 13th International Conference on Fuel Cell Science, Engineering and Technology, and the ASME 2015 Nuclear Forum. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/es2015-49712.

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We investigate losses and costs associated with direct steam generation via parabolidal dish concentrators and steam transport to a central steam Rankine power cycle for electricity generation. This study presents a power plant model that accounts for the effects of shading, steam transport, energy conversion at the power block, and capital costs of land and pipework. The pipe network topology used was optimised using a genetic algorithm based on evolution of minimal spanning trees connecting all dishes to a central power block. Optimal pipe sizing of the network is determined by considering t

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Iino, Kenji, Ritsuo Yoshioka, Masao Fuchigami, and Masayuki Nakao. "What Could Have Saved Fukushima From Its Severe Accident." In ASME 2016 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/imece2016-65069.

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The Great East Japan Earthquake on March 11, 2011 triggered huge tsunami waves that devastated the northeast region of Japan along the Pacific coastline. The Tokyo Electric Power Company (TEPCO) owned Fukushima Daiichi Nuclear Power Plant (Fukushima-1) survived the earthquake, however, not the tsunami that followed. Four of the 6 reactor units underwent Station Blackout. Unit 5 lost all its own AC power, however, it shared AC power with Unit 6. Units 1, 3, and 4 had hydrogen explosions that destroyed their reactor buildings, and even worse, 1, 2, and 3 had core meltdowns to release a large amo

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