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Journal articles on the topic 'Nuclear run-off analysis; Termination'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

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1

Kerppola, T. K., and C. M. Kane. "Intrinsic sites of transcription termination and pausing in the c-myc gene." Molecular and Cellular Biology 8, no. 10 (1988): 4389–94. http://dx.doi.org/10.1128/mcb.8.10.4389.

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We have studied transcription elongation and termination in the human c-myc gene. Transcription of c-myc gene sequences with purified mammalian RNA polymerase II revealed several sites of transcription termination and pausing in the vicinity of the exon 1-intron 1 junction. This region previously has been shown to block transcription elongation in vivo by nuclear run-on analysis (D. Bentley and M. Groudine, Nature [London] 321:702-706, 1986). These sites were recognized by purified RNA polymerase II, and we therefore designated them intrinsic sites of termination and pausing. Two of these site

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2

Kerppola, T. K., and C. M. Kane. "Intrinsic sites of transcription termination and pausing in the c-myc gene." Molecular and Cellular Biology 8, no. 10 (1988): 4389–94. http://dx.doi.org/10.1128/mcb.8.10.4389-4394.1988.

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We have studied transcription elongation and termination in the human c-myc gene. Transcription of c-myc gene sequences with purified mammalian RNA polymerase II revealed several sites of transcription termination and pausing in the vicinity of the exon 1-intron 1 junction. This region previously has been shown to block transcription elongation in vivo by nuclear run-on analysis (D. Bentley and M. Groudine, Nature [London] 321:702-706, 1986). These sites were recognized by purified RNA polymerase II, and we therefore designated them intrinsic sites of termination and pausing. Two of these site

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3

Birkenheuer, Claire H., Charles G. Danko, and Joel D. Baines. "Herpes Simplex Virus 1 Dramatically Alters Loading and Positioning of RNA Polymerase II on Host Genes Early in Infection." Journal of Virology 92, no. 8 (2018): e02184-17. http://dx.doi.org/10.1128/jvi.02184-17.

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ABSTRACTHerpes simplex virus 1 (HSV-1) transcription is mediated by cellular RNA polymerase II (Pol II). Recent studies investigating how Pol II transcription of host genes is altered after HSV-1 are conflicting. Chromatin immunoprecipitation sequencing (ChIP-seq) studies suggest that Pol II is almost completely removed from host genes at 4 h postinfection (hpi), while 4-thiouridine (4SU) labeling experiments show that host transcription termination is extended at 7 hpi, implying that a significant amount of Pol II remains associated with host genes in infected cells. To address this discrepan

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Miller, H., C. Asselin, D. Dufort, et al. "A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block." Molecular and Cellular Biology 9, no. 12 (1989): 5340–49. http://dx.doi.org/10.1128/mcb.9.12.5340.

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A block to elongation of transcription has been shown to occur within the first exon of the human and murine c-myc genes. The extent of this block was found to vary with the physiological state of cells, indicating that modulation of the transcriptional block can serve to control the expression of this gene. To determine which sequences are required in cis for the transcriptional block, we generated a series of constructs containing various portions of murine c-myc 5'-flanking and exon 1 sequences. We established populations of HeLa and CV-1 cells stably transfected with these constructs. The

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Miller, H., C. Asselin, D. Dufort, et al. "A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block." Molecular and Cellular Biology 9, no. 12 (1989): 5340–49. http://dx.doi.org/10.1128/mcb.9.12.5340-5349.1989.

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A block to elongation of transcription has been shown to occur within the first exon of the human and murine c-myc genes. The extent of this block was found to vary with the physiological state of cells, indicating that modulation of the transcriptional block can serve to control the expression of this gene. To determine which sequences are required in cis for the transcriptional block, we generated a series of constructs containing various portions of murine c-myc 5'-flanking and exon 1 sequences. We established populations of HeLa and CV-1 cells stably transfected with these constructs. The

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Napp, Adriane E., Torsten Diekhoff, Olf Stoiber, et al. "Audio-guided self-hypnosis for reduction of claustrophobia during MR imaging: results of an observational 2-group study." European Radiology 31, no. 7 (2021): 4483–91. http://dx.doi.org/10.1007/s00330-021-07887-w.

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Abstract Objectives To evaluate the influence of audio-guided self-hypnosis on claustrophobia in a high-risk cohort undergoing magnetic resonance (MR) imaging. Methods In this prospective observational 2-group study, 55 patients (69% female, mean age 53.6 ± 13.9) used self-hypnosis directly before imaging. Claustrophobia included premature termination, sedation, and coping actions. The claustrophobia questionnaire (CLQ) was completed before self-hypnosis and after MR imaging. Results were compared to a control cohort of 89 patients examined on the same open MR scanner using logistic regression

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Wu, Yanping, Jennifer H. McQuiston, Andrew Cox, Todd D. Pack, and Thomas J. Inzana. "Molecular Cloning and Mutagenesis of a DNA Locus Involved in Lipooligosaccharide Biosynthesis in Haemophilus somnus." Infection and Immunity 68, no. 1 (2000): 310–19. http://dx.doi.org/10.1128/iai.68.1.310-319.2000.

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ABSTRACT Haemophilus somnus undergoes antigenic and structural phase variation in its lipooligosaccharide (LOS). A gene (lob-1) containing repetitive 5′-CAAT-3′ sequences that may, in part, contribute to phase variation was cloned and sequenced (T. J. Inzana et al., Infect. Immun. 65:4675–4681, 1997). We have now identified another putative gene (lob-2A) immediately upstream from lob-1. Lob-2A contained homology to several LOS biosynthesis proteins of the family Pasteurellaceae and the LgtB and LgtE galactosyltransferases of Neisseria meningitidis and N. gonorrhoeae. Unlikelob-1, lob-2A contai

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8

Martínez-Calvillo, Santiago, Dan Nguyen, Kenneth Stuart, and Peter J. Myler. "Transcription Initiation and Termination on Leishmania major Chromosome 3." Eukaryotic Cell 3, no. 2 (2004): 506–17. http://dx.doi.org/10.1128/ec.3.2.506-517.2004.

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ABSTRACT Genome projects involving Leishmania and other trypanosomatids have revealed that most genes in these organisms are organized into large clusters of genes on the same DNA strand. We have previously shown that transcription of the entire Leishmania major Friedlin (LmjF) chromosome 1 (chr1) initiates bidirectionally between two divergent gene clusters. Here, we analyze transcription of LmjF chr3, which contains two convergent clusters of 67 and 30 genes, separated by a tRNA gene, with a single divergent protein-coding gene located close to the “left” telomere. Nuclear run-on analyses in

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9

Shiota, Chiyo, Kazuhiro Ikenaka, and Katsuhiko Mikoshiba. "Developmental Expression of Myelin Protein Genes in Dysmyelinating Mutant Mice: Analysis by Nuclear Run-Off Transcription Assay, In Situ Hybridization, and Immunohistochemistry." Journal of Neurochemistry 56, no. 3 (1991): 818–26. http://dx.doi.org/10.1111/j.1471-4159.1991.tb01997.x.

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10

Falk, G., and J. E. Walker. "Transcription of Rhodospirillum rubrum atp operon." Biochemical Journal 229, no. 3 (1985): 663–68. http://dx.doi.org/10.1042/bj2290663.

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The photosynthetic non-sulphur bacterium Rhodospirillum rubrum contains a cluster of five genes encoding the subunits of F1-ATPase [Falk, Hampe & Walker (1985) Biochem. J. 228, 391-407]. Transcription of these genes has been studied by two methods, transcriptional mapping with S1 nuclease and primer extension analysis. Thereby a 5'-end in RNA derived from this region has been demonstrated at a guanine residue 236 bases before the initiation codon of the gene for the delta-subunit, the first in this cluster. DNA sequences on the 5' side of this nucleotide show some similarity to promoters i

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11

Linn, S. C., P. J. Morelli, I. Edry, S. E. Cottongim, C. Szabo, and A. L. Salzman. "Transcriptional regulation of human inducible nitric oxide synthase gene in an intestinal epithelial cell line." American Journal of Physiology-Gastrointestinal and Liver Physiology 272, no. 6 (1997): G1499—G1508. http://dx.doi.org/10.1152/ajpgi.1997.272.6.g1499.

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The inducible form of nitric oxide synthase (iNOS) is expressed during inflammation of the intestine and may contribute to tissue injury. We have examined iNOS transcriptional regulation in DLD-1 cells, a human intestinal epithelial line that produces large amounts of nitric oxide and iNOS mRNA in response to a combination of the proinflammatory cytokines interleukin-1 beta (IL-1 beta) and interferon-gamma (IFN-gamma). Levels of iNOS mRNA are extremely low in unstimulated DLD-1 cells but increase dramatically after cytokine treatment. Nuclear run-on analyses demonstrated that transcriptional a

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12

Hampson, IN, MA Cross, CM Heyworth, et al. "Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3- dependent murine stem cell lines." Blood 80, no. 12 (1992): 3097–105. http://dx.doi.org/10.1182/blood.v80.12.3097.3097.

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Abstract Using the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme ‘B’), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate l

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Hampson, IN, MA Cross, CM Heyworth, et al. "Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3- dependent murine stem cell lines." Blood 80, no. 12 (1992): 3097–105. http://dx.doi.org/10.1182/blood.v80.12.3097.bloodjournal80123097.

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Using the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme ‘B’), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate leukocytes

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14

HAVERKAMP, SILKE, FRANCOISE HAESELEER, and ANITA HENDRICKSON. "A comparison of immunocytochemical markers to identify bipolar cell types in human and monkey retina." Visual Neuroscience 20, no. 6 (2003): 589–600. http://dx.doi.org/10.1017/s0952523803206015.

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As more human retinas affected with genetic or immune-based diseases become available for morphological analysis, it is important to identify immunocytochemical markers for specific subtypes of retinal neurons. In this study, we have focused on bipolar cell markers in central retina. We have done single and double labeling using several antisera previously utilized in macaque monkey or human retinal studies and two new antisera (1) to correlate combinations of antisera labeling with morphological types of bipolar cells in human retina, and (2) to compare human labeling patterns with those in m

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15

Flowers, M. A., Y. Wang, R. J. Stewart, B. Patel, and P. A. Marsden. "Reciprocal regulation of endothelin-1 and endothelial constitutive NOS in proliferating endothelial cells." American Journal of Physiology-Heart and Circulatory Physiology 269, no. 6 (1995): H1988—H1997. http://dx.doi.org/10.1152/ajpheart.1995.269.6.h1988.

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The expression of endothelin-1 (ET-1) and endothelial constitutive nitric oxide synthase (ecNOS) was assessed in two independent in vitro models: asynchronously differentially proliferating cultures and wounded endothelial cell monolayers. Northern blot analysis of RNA isolated from preconfluent, confluent, and postconfluent cells revealed a fourfold rise in ET-1 mRNA transcripts, whereas levels of ecNOS mRNA transcripts were reduced twofold in proliferating cells. Nuclear run-off analysis demonstrated that increased steady-state ET-1 mRNA content in proliferating cells was mediated, in part,

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16

Batistatou, A., C. Volonté, and L. A. Greene. "Nerve growth factor employs multiple pathways to induce primary response genes in PC12 cells." Molecular Biology of the Cell 3, no. 3 (1992): 363–71. http://dx.doi.org/10.1091/mbc.3.3.363.

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Nerve growth factor (NGF) leads to neuronal differentiation of PC12 cells and promotes their survival in serum-free medium. Past studies have shown that purine analogues block some of the effects of NGF but not others and thus that they can be used to dissect the mechanistic pathways of its action. In the present work we used 2-aminopurine (2-AP) and 6-thioguanine (6-TG) to examine whether NGF causes activation of primary response genes through a single signaling pathway or via multiple pathways. Northern blot analysis and nuclear run-off transcription assays were used to assess the activation

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17

Granneman, J. G., and K. N. Lahners. "Regulation of mouse beta 3-adrenergic receptor gene expression and mRNA splice variants in adipocytes." American Journal of Physiology-Cell Physiology 268, no. 4 (1995): C1040—C1044. http://dx.doi.org/10.1152/ajpcell.1995.268.4.c1040.

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This study examined the regulation of murine beta 3-receptor mRNA and determined whether the recently described mRNA splice variants are differentially regulated by agents that alter total beta 3-receptor mRNA levels. In vivo treatment of mice with the beta 3-receptor agonist BRL-26830 reduced total beta 3-transcripts by 64% in white adipose tissue but did not alter the mRNA splicing pattern. Further analysis in cultured 3T3-F442A adipocytes showed that isoproterenol, dexamethasone, or phorbol 12-myristate 13-acetate also greatly reduced beta 3-receptor mRNA levels without selectively altering

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18

Schulte, L., J. C. Peña, F. Carvalho, et al. "A 2600 year history of floods in the Bernese Alps, Switzerland: frequencies, mechanisms and climate forcing." Hydrology and Earth System Sciences Discussions 12, no. 3 (2015): 3391–448. http://dx.doi.org/10.5194/hessd-12-3391-2015.

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Abstract. A 2600 yr long composite palaeoflood record is reconstructed from high-resolution delta plain sediments of the Hasli–Aare floodplain on the northern slope of the Swiss Alps. Natural proxies compiled from sedimentary, geochemical and geomorphological data were calibrated by textual and factual sources and instrumental data. No fewer than 12 of the 14 historically recorded extreme events between 1480 and the termination of the Hasli–Aare Correction in 1875 were also identified by coarse-grained flood layers, log(Zr/Ti) peaks and Factor 1 anomalies. Geomorphological, historical and inst

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19

Yamasaki, Toshiki, George Klein, Hans-Gustaf Ljunggren, et al. "Role of histocompatibility antigen gene and proto-oncogene expressions in intracerebral tumorigenicity of mouse neuroblastoma." Journal of Neurosurgery 78, no. 4 (1993): 619–29. http://dx.doi.org/10.3171/jns.1993.78.4.0619.

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✓ The role of N-myc, c-src, and major histocompatibility complex (MHC, H-2 in the mouse) class I antigen gene expressions in dimethyl sulfoxide (DMSO)-induced differentiation and intracerebral tumorigenicity was examined using a mouse MNB85 neuroblastoma cell line. A fluorescence-activated cell sorter disclosed cell-surface MHC enhancement by DMSO, causing an increase in cytotoxic T-lymphocyte sensitivity. Southern blot analysis verified a single copy of the proto-oncogenes and MHC deoxyribonucleic acids in both untreated and DMSO-treated MNB85 cells. Northern blot analysis indicated that DMSO

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20

Aklilu, F., M. Park, D. Goltzman, and S. A. Rabbani. "Increased PTHRP production by a tyrosine kinase oncogene, Tpr-Met: role of the Ras signaling pathway." American Journal of Physiology-Endocrinology and Metabolism 271, no. 2 (1996): E277—E283. http://dx.doi.org/10.1152/ajpendo.1996.271.2.e277.

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We have used the Tpr-Met oncogene as a model to examine signaling pathways of growth factors and tyrosine kinase oncogenes that can increase parathyroid hormone-related peptide (PTHRP) production. PTHRP production in Tpr-Met transfected cells, when assessed by Northern blot analysis and radioimmunoassay, was increased four- to eightfold. Treatment of these cells with the transcriptional inhibitor actinomycin D and nuclear run-off assays showed that the major cause of increased PTHRP mRNA was enhanced gene transcription. To analyze the intracellular signaling molecules involved in PTHRP product

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21

Rupprecht, H. D., V. P. Sukhatme, J. Lacy, R. B. Sterzel, and D. L. Coleman. "PDGF-induced Egr-1 expression in rat mesangial cells is mediated through upstream serum response elements." American Journal of Physiology-Renal Physiology 265, no. 3 (1993): F351—F360. http://dx.doi.org/10.1152/ajprenal.1993.265.3.f351.

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Platelet-derived growth factor (PDGF) has been implicated in the process of mesangial cell (MC) proliferation in vitro and in vivo. To investigate early changes in gene expression that couple biochemical events with changes in phenotype in PDGF-stimulated cultured MC, we studied expression of the early growth response gene 1 (Egr-1), a member of the family of immediate early genes. Our findings show that protein tyrosine phosphorylation is required for induction of Egr-1 mRNA and proliferation by PDGF in MC. Nuclear run-off assays show that Egr-1 induction occurs at the transcriptional level.

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Mueller, Xavier M., Monique Augstburger, Yves Boone, and Ludwig K. von Segesser. "Hemocompatibility of a coaxial pump catheter for less invasive heart surgery." Perfusion 17, no. 1 (2002): 3–7. http://dx.doi.org/10.1191/0267659102pf529oa.

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Off-pump coronary artery bypass (OPCAB) requires heart manipulation during exposure of the lateral and posterior walls of the heart, which may cause hemodynamic instability, mainly through right ventricular dysfunction. A coaxial atrial cannula connected to a minicentrifugal pump was developed to bypass the right heart. This study was designed to test the hemocompatibility of this pump ongoing for 6 h. In five calves (bodyweight, 70.3± 4.2 kg), the pump was inserted and set to its maximal motor speed of 7000 rpm. Blood samples were taken for blood gas analyses, hematology and chemistry on an h

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23

Naora, H., and IG Young. "Mechanisms regulating the mRNA levels of interleukin-5 and two other coordinately expressed lymphokines in the murine T lymphoma EL4.23." Blood 83, no. 12 (1994): 3620–28. http://dx.doi.org/10.1182/blood.v83.12.3620.3620.

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Abstract The mechanisms that regulate the mRNA levels of interleukin-5 (IL-5) were compared with those regulating the mRNA levels of two other coordinately expressed lymphokines in the murine T lymphoma EL4.23. Our results indicate that IL-5 mRNA levels are independently regulated from those of IL-2 and granulocyte-macrophage colony-stimulating factor (GM- CSF) mRNAs. The induction of IL-5 mRNA by phorbol 12-myristate 13- acetate (PMA) stimulation was found to be cyclosporin A-resistant, in contrast to the induction of IL-2 and GM-CSF mRNAs. Although the three lymphokine mRNAs were not detecte

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Naora, H., and IG Young. "Mechanisms regulating the mRNA levels of interleukin-5 and two other coordinately expressed lymphokines in the murine T lymphoma EL4.23." Blood 83, no. 12 (1994): 3620–28. http://dx.doi.org/10.1182/blood.v83.12.3620.bloodjournal83123620.

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The mechanisms that regulate the mRNA levels of interleukin-5 (IL-5) were compared with those regulating the mRNA levels of two other coordinately expressed lymphokines in the murine T lymphoma EL4.23. Our results indicate that IL-5 mRNA levels are independently regulated from those of IL-2 and granulocyte-macrophage colony-stimulating factor (GM- CSF) mRNAs. The induction of IL-5 mRNA by phorbol 12-myristate 13- acetate (PMA) stimulation was found to be cyclosporin A-resistant, in contrast to the induction of IL-2 and GM-CSF mRNAs. Although the three lymphokine mRNAs were not detected in unst

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Fincato, G., N. Polentarutti, A. Sica, A. Mantovani, and F. Colotta. "Expression of a heat-inducible gene of the HSP70 family in human myelomonocytic cells: regulation by bacterial products and cytokines." Blood 77, no. 3 (1991): 579–86. http://dx.doi.org/10.1182/blood.v77.3.579.579.

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Abstract In this study we have examined the expression of a heat-shock protein (HSP) 70 gene in normal human peripheral blood leukocytes. Northern blot analysis showed that appreciable levels of hsp70 mRNA are present in monocytes and granulocytes, whereas transcript levels were barely detectable or absent in lymphocytes. Monocytes functionally activated by bacterial lipopolysaccharide (LPS) showed an early (15 minutes) increase of hsp70 transcripts that was shown, by actinomycin D blocking and nuclear run-off experiments, to be dependent on transcriptional activation of the gene. LPS did not

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26

Fincato, G., N. Polentarutti, A. Sica, A. Mantovani, and F. Colotta. "Expression of a heat-inducible gene of the HSP70 family in human myelomonocytic cells: regulation by bacterial products and cytokines." Blood 77, no. 3 (1991): 579–86. http://dx.doi.org/10.1182/blood.v77.3.579.bloodjournal773579.

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In this study we have examined the expression of a heat-shock protein (HSP) 70 gene in normal human peripheral blood leukocytes. Northern blot analysis showed that appreciable levels of hsp70 mRNA are present in monocytes and granulocytes, whereas transcript levels were barely detectable or absent in lymphocytes. Monocytes functionally activated by bacterial lipopolysaccharide (LPS) showed an early (15 minutes) increase of hsp70 transcripts that was shown, by actinomycin D blocking and nuclear run-off experiments, to be dependent on transcriptional activation of the gene. LPS did not appreciab

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27

Kaisar, Jeremy, Aaron Chen, Mathew Cheung, Elias Kfoury, Carlos F. Bechara, and Peter H. Lin. "Comparison of propaten heparin-bonded vascular graft with distal anastomotic patch versus autogenous saphenous vein graft in tibial artery bypass." Vascular 26, no. 2 (2017): 117–25. http://dx.doi.org/10.1177/1708538117717141.

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Introduction Heparin-bonded expanded polytetrafluoroethylene grafts (Propaten, WL Gore, Flagstaff, AZ, USA) have been shown to have superior patency compared to standard prosthetic grafts in leg bypass. This study analyzed the outcomes of Propaten grafts with distal anastomotic patch versus autogenous saphenous vein grafts in tibial artery bypass. Methods A retrospective analysis of prospective collected data was performed during a recent 15-year period. Sixty-two Propaten bypass grafts with distal anastomotic patch (Propaten group) were compared with 46 saphenous vein graft (vein group). Pert

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Hahnemann, B., P. Salonpää, M. Pasanen, et al. "Effect of pyrazole, cobalt and phenobarbital on mouse liver cytochrome P-450 2a-4/5 (Cyp2a-4/5) expression." Biochemical Journal 286, no. 1 (1992): 289–94. http://dx.doi.org/10.1042/bj2860289.

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Pyrazole, cobalt and phenobarbital increase the activity of coumarin 7-hydroxylase (COH) in mouse liver. To study the mechanism of this increase, we measured the expression of the cytochrome P-450 2a-4/5 (Cyp2a-4/5) complex, which mediates testosterone 15 alpha-hydroxylase and COH activities, as a function of dose and time after the treatment of C57BL/6 (B6) and DBA/2 (D2) male mice with the inducers. COH activity and Cyp2a-4/5 steady-state mRNA levels were increased in both strains in response to the inducers. No marked effect occurred with testosterone 15 alpha-hydroxylase or activities asso

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Mitani, K., H. Fujita, Y. Fukuda, A. Kappas, and S. Sassa. "The role of inorganic metals and metalloporphyrins in the induction of haem oxygenase and heat-shock protein 70 in human hepatoma cells." Biochemical Journal 290, no. 3 (1993): 819–25. http://dx.doi.org/10.1042/bj2900819.

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The role of inorganic metals and metalloporphyrins in the induction of mRNAs for haem oxygenase and heat-shock protein 70 (hsp70), the two heat-shock proteins, was examined in human HepG2 and Hep3B hepatoma cells. SnCl2, but not Sn-protoporphyrin, was found to be a potent inducer of both haem oxygenase and hsp70 mRNAs. In contrast, CoCl2, ZnCl2 and FeCl2 caused little induction of haem oxygenase and hsp70 mRNAs, whereas the porphyrin complexes of these metals strongly induced haem oxygenase mRNA, without influencing the level of hsp70 mRNA. The induction process was largely transcriptional, as

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30

Ohlsson, M., G. Leonardsson, X. C. Jia, P. Feng, and T. Ny. "Transcriptional regulation of the rat tissue type plasminogen activator gene: localization of DNA elements and nuclear factors mediating constitutive and cyclic AMP-induced expression." Molecular and Cellular Biology 13, no. 1 (1993): 266–75. http://dx.doi.org/10.1128/mcb.13.1.266.

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We have characterized tissue type plasminogen activator (tPA) promoter elements and nuclear factors required for follicle-stimulating hormone (FSH)-induced transcription of the rat tPA gene in granulosa cells and constitutive expression of the gene in the rat neuroblastoma cell line B103. Run-on transcription analysis of isolated nuclei revealed that B103 cells transcribe the tPA gene at a high and constitutive level, while FSH was found to induce tPA gene transcription in a rapid and transient manner in granulosa cells. The maximal FSH-induced transcription rate was obtained after 20 min and

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Ohlsson, M., G. Leonardsson, X. C. Jia, P. Feng, and T. Ny. "Transcriptional regulation of the rat tissue type plasminogen activator gene: localization of DNA elements and nuclear factors mediating constitutive and cyclic AMP-induced expression." Molecular and Cellular Biology 13, no. 1 (1993): 266–75. http://dx.doi.org/10.1128/mcb.13.1.266-275.1993.

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We have characterized tissue type plasminogen activator (tPA) promoter elements and nuclear factors required for follicle-stimulating hormone (FSH)-induced transcription of the rat tPA gene in granulosa cells and constitutive expression of the gene in the rat neuroblastoma cell line B103. Run-on transcription analysis of isolated nuclei revealed that B103 cells transcribe the tPA gene at a high and constitutive level, while FSH was found to induce tPA gene transcription in a rapid and transient manner in granulosa cells. The maximal FSH-induced transcription rate was obtained after 20 min and

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32

Hinneburg, Alexander, Björn Egert, and Andrea Porzel. "Duplicate detection of 2D-NMR Spectra." Journal of Integrative Bioinformatics 4, no. 1 (2007): 64–80. http://dx.doi.org/10.1515/jib-2007-53.

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Abstract 2D-Nuclear magnetic resonance (NMR) spectra are used in the (structural) analysis of small molecules. In contrast to 1D-NMR spectra, 2D-NMR spectra correlate the chemical shifts of 1H and 13C at the same time. A spectrum consists of several peaks in a two--dimensional space. The most important information of a peak is the location of its center, which captures the bonding relationships of hydrogen and carbon atoms. A spectrum contains much information about the chemical structure of a product, but in most cases the structure cannot be read off in a simple and straightforward manner. S

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Hurme, M., E. Serkkola, T. Ronni, and O. Silvennoinen. "Control of interleukin-1 beta expression by protein kinase C and cyclic adenosine monophosphate in myeloid leukemia cells." Blood 76, no. 11 (1990): 2198–203. http://dx.doi.org/10.1182/blood.v76.11.2198.2198.

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Abstract We have examined the signal transduction pathways leading to the expression of the interleukin-1 beta (IL-1 beta) gene in human myeloid leukemia cells lines. Two cell lines representing different stages of differentiation were used (HL-60, promyelocytic, and THP-1, mature monocytic). In accordance with previous studies, it was observed that a protein kinase C (PKC) activator, phorbol myristate acetate (PMA), was a sufficient stimulus for induction of the IL-1 beta messenger RNA (mRNA) expression and IL-1 beta protein production in both of these cell lines. A structural analog of cycli

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Hurme, M., E. Serkkola, T. Ronni, and O. Silvennoinen. "Control of interleukin-1 beta expression by protein kinase C and cyclic adenosine monophosphate in myeloid leukemia cells." Blood 76, no. 11 (1990): 2198–203. http://dx.doi.org/10.1182/blood.v76.11.2198.bloodjournal76112198.

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We have examined the signal transduction pathways leading to the expression of the interleukin-1 beta (IL-1 beta) gene in human myeloid leukemia cells lines. Two cell lines representing different stages of differentiation were used (HL-60, promyelocytic, and THP-1, mature monocytic). In accordance with previous studies, it was observed that a protein kinase C (PKC) activator, phorbol myristate acetate (PMA), was a sufficient stimulus for induction of the IL-1 beta messenger RNA (mRNA) expression and IL-1 beta protein production in both of these cell lines. A structural analog of cyclic adenosi

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Frigon, NL Jr, L. Shao, AL Young, L. Maderazo, and J. Yu. "Regulation of globin gene expression in human K562 cells by recombinant activin A." Blood 79, no. 3 (1992): 765–72. http://dx.doi.org/10.1182/blood.v79.3.765.765.

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Abstract Recent studies indicate that a purified protein, activin A, belongs to the transforming growth factor beta (TGF-beta) superfamily. Similar to TGF-beta, activin A can have different biologic activities, depending on the target tissues. We used recombinant activin A to demonstrate a possible regulatory role of this protein in modulating human erythroid differentiation in the human erythroid cell line, K562. Using genomic probes containing the second exon of alpha, beta, gamma, and epsilon globins, relative abundance of various types of globin transcripts in untreated and activin-treated

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Frigon, NL Jr, L. Shao, AL Young, L. Maderazo, and J. Yu. "Regulation of globin gene expression in human K562 cells by recombinant activin A." Blood 79, no. 3 (1992): 765–72. http://dx.doi.org/10.1182/blood.v79.3.765.bloodjournal793765.

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Recent studies indicate that a purified protein, activin A, belongs to the transforming growth factor beta (TGF-beta) superfamily. Similar to TGF-beta, activin A can have different biologic activities, depending on the target tissues. We used recombinant activin A to demonstrate a possible regulatory role of this protein in modulating human erythroid differentiation in the human erythroid cell line, K562. Using genomic probes containing the second exon of alpha, beta, gamma, and epsilon globins, relative abundance of various types of globin transcripts in untreated and activin-treated K562 cel

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SCHUPPIN, George T., та Christopher J. RHODES. "Specific co-ordinated regulation of PC3 and PC2 gene expression with that of preproinsulin in insulin-producing β TC3 cells". Biochemical Journal 313, № 1 (1996): 259–68. http://dx.doi.org/10.1042/bj3130259.

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Short-term (less than 2 h) glucose stimulation of isolated pancreatic islets specifically increases the biosynthesis of proinsulin and its converting enzymes PC2 and PC3 at the translation level. To determine whether gene expression of PC2 and PC3 was also regulated by longer-term (more than 6 h) glucose stimulation along with that of preproinsulin, studies were performed with the βTC3 insulin-producing cell line. By Northern blot analysis, glucose maintained PC2 and PC3 mRNA levels in parallel with those of preproinsulin. After 48 h, mRNA levels of preproinsulin, PC2 and PC3 were, respectivel

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Ramsey, Haley, Susu Zhang, Yue Zhao, et al. "The BET Inhibitor INCB054329 Primes AML Cells for Venetoclax-Induced Apoptosis." Blood 132, Supplement 1 (2018): 4074. http://dx.doi.org/10.1182/blood-2018-99-111393.

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Abstract Bromodomain and extra-terminal (BET) inhibitors may be efficacious for treatment of acute myeloid leukemia because they attenuate the expression of critical oncogenes including MYC and BCL2. These BET inhibitors (BETi) disrupt the transcriptional elongation process by displacing BET family members BRD2,3, and 4 off of chromatin, and causing RNA polymerase promoter-proximal pausing. We used precision nuclear run-on transcription sequencing (PROseq) to directly measure the effects of INCB054329, a potent BETi, on RNA polymerase II pausing and elongation. We found dramatic reductions on

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Rallidis, L., K. Papangelopoulou, A. Anthi, et al. "P1790 Is there any role of exercise Doppler echocardiography to unmask pulmonary hypertension (PH) in patients with systemic sclerosis and baseline echocardiographic measurements for PH in the gray zone?" European Heart Journal - Cardiovascular Imaging 21, Supplement_1 (2020). http://dx.doi.org/10.1093/ehjci/jez319.1146.

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Abstract Background The usefulness of exercise Doppler echocardiography (EDE) is uncertain in identifying patients with pulmonary hypertension (PH). Recently the cut off value for the definition of PH was changed and was set at the level of mean pulmonary arterial pressure (PAP) &gt;20 mmHg, measured by right heart catheterization (RHC). Purpose We examined whether EDE can unmask the presence of PH in patients with systemic sclerosis (SSc) whose baseline echocardiographic assessment for PH is non-diagnostic. Methods Forty-one patients with SSc (2 men and 39 women; mean age 61.2 ± 10 years)

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Okuno, Taishi, Jonas Lanz, Stefan Stortecky, et al. "Clinical impact of left atrial appendage filling defects in patients undergoing transcatheter aortic valve implantation." European Heart Journal - Cardiovascular Imaging, August 31, 2021. http://dx.doi.org/10.1093/ehjci/jeab142.

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Abstract Aims Incidental detection of left atrial appendage (LAA) filling defects is a common finding on multi-detector computed tomography in aortic stenosis patients under evaluation for transcatheter aortic valve implantation (TAVI). We aimed to investigate the incidence of LAA filling defects before TAVI and its impact on clinical outcomes. Methods and results In a prospective registry, LAA filling defects were retrospectively evaluated and categorized into one of four sub-types: thrombus-like, heterogeneous, horizontal, and Hounsfield Unit (HU)-run-off. The primary endpoint was the compos

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Bakhti, Pouya, Yasaman Farzan та Silvia Pascoli. "Discovery potential of FASERν with contained vertex and through-going events". Journal of High Energy Physics 2021, № 4 (2021). http://dx.doi.org/10.1007/jhep04(2021)075.

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Abstract FASERν is a newly proposed detector whose main mission is to detect the neutrino flux from the collision of the proton beams at the ATLAS Interaction Point (IP) during the run III of the LHC in 2022–2024. We show that this detector can also test certain beyond standard model scenarios, especially the ones in which the neutrino interaction with matter fields can produce new unstable particles decaying back into charged leptons. Models of this kind are motivated by the MiniBooNE anomaly. We show that, if the new physics involves multi-muon production by neutrinos scattering off matter f

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d'Ambrosio, Stefano, Alessandro Ferrari, and Ezio Spessa. "Analysis of the Exhaust Gas Recirculation System Performance in Modern Diesel Engines." Journal of Engineering for Gas Turbines and Power 135, no. 8 (2013). http://dx.doi.org/10.1115/1.4024089.

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Exhaust gas recirculation (EGR) is extensively employed in diesel combustion engines to achieve nitrogen oxides emission targets. The EGR is often cooled in order to increase the effectiveness of the strategy, even though this leads to a further undesired impact on particulate matter and hydrocarbons. Experimental tests were carried out on a diesel engine at a dynamometer rig under steady-state speed and load working conditions that were considered relevant for the New European Driving Cycle. Two different shell and tube-type EGR coolers were compared, in terms of the pressure and temperature

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Normahani, Pasha, Ismail Yusuf Anwar, Alona Courtney, et al. "Factors associated with infrainguinal bypass graft patency at 1-year; a retrospective analysis of a single centre experience." Perfusion, February 26, 2021, 026765912199576. http://dx.doi.org/10.1177/0267659121995760.

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Introduction: The aim of this study was to identify factors associated with primary graft patency 1 year following open lower limb revascularisation (LLR) at a tertiary referral vascular service. Methods: A retrospective analysis of patients undergoing infra-inguinal bypass surgery between January 2016 and May 2017 at a tertiary vascular centre (St Mary’s Hospital, London) was performed. Data regarding patient demographics, comorbidities, type of operation and post-operative anti-thrombotic strategy were collected. Quality of run-off score was assessed from pre-operative imaging. Results: Seve

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Mezzetto, Luca, Davide Mastrorilli, Lorenzo Scorsone, et al. "Early and midterm outcomes of hybrid first line treatment in patients with chronic limb threatening ischemia." Vascular, August 20, 2021, 170853812110409. http://dx.doi.org/10.1177/17085381211040986.

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Objective The aim of the study was to evaluate the outcome of first line hybrid treatment in patients with chronic limb threatening ischemia (CLTI) and to evaluate possible predictors of primary patency (PP) loss and limb clinical improvement (LCI). Methods This was a retrospective non-randomized study. All patients underwent one-stage hybrid common femoral artery (CFA) endarterectomy combined with an inflow and/or outflow endovascular revascularization procedure. Demographic, clinical, and lesion characteristics for each patient were reported. Primary patency analysis was performed using Kapl

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Adjei, Richard Amankwa, WeiZhe Wang, and YingZheng Liu. "Aerodynamic Design Optimization of an Axial Flow Compressor Stator Using Parameterized Free-Form Deformation." Journal of Engineering for Gas Turbines and Power 141, no. 10 (2019). http://dx.doi.org/10.1115/1.4044692.

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AbstractThis paper describes an aerodynamic design optimization of a highly loaded compressor stator blade using parameterized free-form deformation (FFD). The optimization methodology presented utilizes a B-spline-based FFD control volume to map the blade from the object space to the parametric space via transformation operations in order to perturb the blade surface. Coupled with a multi-objective genetic algorithm (MOGA) and a Gaussian process-based response surface method (RSM), a fully automated iterative loop was used to run the optimization on a fitted correlation function. A weighted a

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Keogh, Luke. "The First Four Wells: Unconventional Gas in Australia." M/C Journal 16, no. 2 (2013). http://dx.doi.org/10.5204/mcj.617.

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Unconventional energy sources have become increasingly important to the global energy mix. These include coal seam gas, shale gas and shale oil. The unconventional gas industry was pioneered in the United States and embraced following the first oil shock in 1973 (Rogers). As has been the case with many global resources (Hiscock), many of the same companies that worked in the USA carried their experience in this industry to early Australian explorations. Recently the USA has secured significant energy security with the development of unconventional energy deposits such as the Marcellus shale ga

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Stooksbury, Kara E., Lori Maxwell, and Cynthia S. Brown. ""Spin Zones" in American Presidential Elections." M/C Journal 14, no. 5 (2011). http://dx.doi.org/10.5204/mcj.410.

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If one morning I walked on top of the water across the Potomac River, the headline that afternoon would read: "President Can't Swim". —Lyndon B. Johnson Introduction The term “spin” implies manipulating the truth, and this concept, along with “spin doctoring,” is now common in media and public discourse. The prevalence of “spin zones” in American politics is undeniable; media outlets themselves, such as Bill O’Reilly’s “No Spin Zone” on Fox News, now run segments on the topic. Despite this apparent media certainty about what constitutes “spin” there is a lack of conceptual clarity regarding th

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